Restriction map for
pF1KSDA0478
(Circular) (Six-base) MAPSORT of: pF1KSDA0478.seq Check: 8164 from: 1 to: 6856
pF1KSDA0478 6856 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2488 3639 3678 3717 2488
Size: 1151 39 39 5627
Fragments arranged by size:
5627 1151 39 39
AccI GT'mk_AC
Cuts at: 423 1130 3892 423
Size: 707 2762 3387
Fragments arranged by size:
3387 2762 707
Acc65I G'GTAC_C
Cuts at: 217 3191 3870 217
Size: 2974 679 3203
Fragments arranged by size:
3203 2974 679
AclI AA'CG_TT
Cuts at: 6696 6696
Size: 6856
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 599 776 1382 2012 2285 2698 2995 3069
Size: 386 177 606 630 273 413 297 74
Cuts at: 3069 3126 4679 5111 5860 6128 213
Size: 57 1553 432 749 268 941
Fragments arranged by size:
1553 941 749 630 606 432 413 386
297 273 268 177 74 57
AfeI AGC'GCT
Cuts at: 2395 2395
Size: 6856
AflIII A'CryG_T
Cuts at: 2548 3070 4206 5312 6289 2548
Size: 522 1136 1106 977 3115
Fragments arranged by size:
3115 1136 1106 977 522
AgeI A'CCGG_T
Cuts at: 5244 5244
Size: 6856
AleI CACnn'nnGTG
Cuts at: 3264 3264
Size: 6856
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2437 2437
Size: 6856
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 175 207 2698 2730 175
Size: 32 2491 32 4301
Fragments arranged by size:
4301 2491 32 32
AlwNI CAG_nnn'CTG
Cuts at: 156 165 1948 2317 2900 3275 4159 5728 156
Size: 9 1783 369 583 375 884 1569 1284
Fragments arranged by size:
1783 1569 1284 884 583 375 369 9
ApaI G_GGCC'C
Cuts at: 634 634
Size: 6856
ApaLI G'TGCA_C
Cuts at: 2383 3040 5626 2383
Size: 657 2586 3613
Fragments arranged by size:
3613 2586 657
ApoI r'AATT_y
Cuts at: 690 849 1267 1341 2179 2569 3858 6209 6465
Size: 159 418 74 838 390 1289 2351 256
Cuts at: 6465 690
Size: 1081
Fragments arranged by size:
2351 1289 1081 838 418 390 256 159
74
AseI AT'TA_AT
Cuts at: 20 20
Size: 6856
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6856
AvaI C'yCGr_G
Cuts at: 328 3874 328
Size: 3546 3310
BamHI G'GATC_C
Cuts at: 3879 3879
Size: 6856
BanI G'GyrC_C
Cuts at: 217 1801 3191 3870 4284 4532 4567 217
Size: 1584 1390 679 414 248 35 2506
Fragments arranged by size:
2506 1584 1390 679 414 248 35
BanII G_rGCy'C
Cuts at: 134 237 328 634 2510 3374 3542 3671 3779
Size: 103 91 306 1876 864 168 129 108
Cuts at: 3779 3868 4898 134
Size: 89 1030 2092
Fragments arranged by size:
2092 1876 1030 864 306 168 129 108
103 91 89
BbeI G_GCGC'C
Cuts at: 4288 4536 4288
Size: 248 6608
BbsI GAAGACnn'nnnn_
Cuts at: 811 867 1375 1420 2234 811
Size: 56 508 45 814 5433
Fragments arranged by size:
5433 814 508 56 45
BbvCI CC'TCA_GC
Cuts at: 1382 1382
Size: 6856
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 319 353 319
Size: 34 6822
BciVI GTATCCnnnnn_n'
Cuts at: 4752 5515 6856 4752
Size: 763 1341 4752
Fragments arranged by size:
4752 1341 763
BclI T'GATC_A
Cuts at: 2253 3692 4374 2253
Size: 1439 682 4735
Fragments arranged by size:
4735 1439 682
BfrBI ATG'CAT
Cuts at: 313 2806 313
Size: 2493 4363
BglII A'GATC_T
Cuts at: 3080 4369 6408 3080
Size: 1289 2039 3528
Fragments arranged by size:
3528 2039 1289
BlpI GC'TnA_GC
Cuts at: 2014 3966 2014
Size: 1952 4904
Bme1580I G_kGCm'C
Cuts at: 634 2387 3044 3093 4479 4572 5630 634
Size: 1753 657 49 1386 93 1058 1860
Fragments arranged by size:
1860 1753 1386 1058 657 93 49
BmrI ACTGGGnnnn_n'
Cuts at: 2829 4066 4147 4482 6628 2829
Size: 1237 81 335 2146 3057
Fragments arranged by size:
3057 2146 1237 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3060 5221 3060
Size: 2161 4695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 608 1387 1631 2503 3124 3247 3494 608
Size: 779 244 872 621 123 247 3970
Fragments arranged by size:
3970 872 779 621 247 244 123
Bpu10I CC'TnA_GC
Cuts at: 411 1382 411
Size: 971 5885
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 4030 4909 5403 5701 542
Size: 3488 879 494 298 1697
Fragments arranged by size:
3488 1697 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1224 2437 3514 3700 6560 1224
Size: 1213 1077 186 2860 1520
Fragments arranged by size:
2860 1520 1213 1077 186
BsaAI yAC'GTr
Cuts at: 2859 3071 3846 4207 2859
Size: 212 775 361 5508
Fragments arranged by size:
5508 775 361 212
BsaBI GATnn'nnATC
Cuts at: 6 4368 6407 6
Size: 4362 2039 455
Fragments arranged by size:
4362 2039 455
BsaHI Gr'CG_yC
Cuts at: 4285 4533 5237 6733 4285
Size: 248 704 1496 4408
Fragments arranged by size:
4408 1496 704 248
BsaWI w'CCGG_w
Cuts at: 3020 3437 4043 4564 5244 5518 5665 6076 3020
Size: 417 606 521 680 274 147 411 3800
Fragments arranged by size:
3800 680 606 521 417 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 175 205 379 409 175
Size: 30 174 30 6622
Fragments arranged by size:
6622 174 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 346 1174 1390 1942 1945 3718 346
Size: 828 216 552 3 1773 3484
Fragments arranged by size:
3484 1773 828 552 216 3
BseYI C'CCAG_C
Cuts at: 732 3428 3569 3710 4279 5037 5616 6112 732
Size: 2696 141 141 569 758 579 496 1476
Fragments arranged by size:
2696 1476 758 579 569 496 141 141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 2088 2443 2457 2516 2929 3009 3288 1159
Size: 929 355 14 59 413 80 279 4727
Fragments arranged by size:
4727 929 413 355 279 80 59 14
BsiEI CG_ry'CG
Cuts at: 104 4396 4442 5652 104
Size: 4292 46 1210 1308
Fragments arranged by size:
4292 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 134 1156 1937 2387 2615 3044 3308 3374 3506
Size: 1022 781 450 228 429 264 66 132
Cuts at: 3506 3542 3599 3616 3779 3868 4646 4836 5630
Size: 36 57 17 163 89 778 190 794
Cuts at: 5630 134
Size: 1360
Fragments arranged by size:
1360 1022 794 781 778 450 429 264
228 190 163 132 89 66 57 36
17
BsmI GAATG_Cn'
Cuts at: 726 997 1967 3417 726
Size: 271 970 1450 4165
Fragments arranged by size:
4165 1450 970 271
BsmBI CGTCTCn'nnnn_
Cuts at: 1518 1518
Size: 6856
Bsp1286I G_dGCh'C
Cuts at: 134 237 328 634 1156 1937 2387 2510 2615
Size: 103 91 306 522 781 450 123 105
Cuts at: 2615 3044 3093 3308 3374 3506 3542 3599 3616
Size: 429 49 215 66 132 36 57 17
Cuts at: 3616 3671 3779 3868 4479 4572 4646 4836 4898
Size: 55 108 89 611 93 74 190 62
Cuts at: 4898 5630 134
Size: 732 1360
Fragments arranged by size:
1360 781 732 611 522 450 429 306
215 190 132 123 108 105 103 93
91 89 74 66 62 57 55 49
36 17
BspEI T'CCGG_A
Cuts at: 6076 6076
Size: 6856
BspHI T'CATG_A
Cuts at: 1533 2735 6032 1533
Size: 1202 3297 2357
Fragments arranged by size:
3297 2357 1202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1778 2488 3387 3639 3678 3717 3904 4118 4420
Size: 710 899 252 39 39 187 214 302
Cuts at: 4420 4801 1778
Size: 381 3833
Fragments arranged by size:
3833 899 710 381 302 252 214 187
39 39
BsrBI CCG'CTC
Cuts at: 50 3108 5146 5200 6686 6851 50
Size: 3058 2038 54 1486 165 55
Fragments arranged by size:
3058 2038 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3478 4766 3478
Size: 1288 5568
BsrFI r'CCGG_y
Cuts at: 146 4852 5033 5244 146
Size: 4706 181 211 1758
Fragments arranged by size:
4706 1758 211 181
BsrGI T'GTAC_A
Cuts at: 167 359 167
Size: 192 6664
BssSI C'ACGA_G
Cuts at: 3778 5125 5485 3778
Size: 1347 360 5149
Fragments arranged by size:
5149 1347 360
BstAPI GCAn_nnn'nTGC
Cuts at: 156 1731 2729 2987 3331 4231 156
Size: 1575 998 258 344 900 2781
Fragments arranged by size:
2781 1575 998 900 344 258
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6856
BstEII G'GTnAC_C
Cuts at: 894 3117 894
Size: 2223 4633
BstYI r'GATC_y
Cuts at: 1 2967 3080 3152 3879 4346 4369 4704 4950
Size: 2966 113 72 727 467 23 335 246
Cuts at: 4950 5964 6050 6062 6408 1
Size: 1014 86 12 346 449
Fragments arranged by size:
2966 1014 727 467 449 346 335 246
113 86 72 23 12
Bsu36I CC'TnA_GG
Cuts at: 1861 2583 1861
Size: 722 6134
BtgI C'CryG_G
Cuts at: 125 2024 2490 3497 3671 125
Size: 1899 466 1007 174 3310
Fragments arranged by size:
3310 1899 1007 466 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4804 4985 5148 4804
Size: 181 163 6512
Fragments arranged by size:
6512 181 163
BtsI GCAGTG_nn'
Cuts at: 1829 2015 2076 2431 2445 2727 4097 1829
Size: 186 61 355 14 282 1370 4588
Fragments arranged by size:
4588 1370 355 282 186 61 14
ClaI AT'CG_AT
Cuts at: 308 308
Size: 6856
DraI TTT'AAA
Cuts at: 911 3853 911
Size: 2942 3914
DraIII CAC_nnn'GTG
Cuts at: 176 3117 176
Size: 2941 3915
DrdI GACnn_nn'nnGTC
Cuts at: 4560 5420 4560
Size: 860 5996
EaeI y'GGCC_r
Cuts at: 2042 4439 4613 5004 5031 6378 2042
Size: 2397 174 391 27 1347 2520
Fragments arranged by size:
2520 2397 1347 391 174 27
EagI C'GGCC_G
Cuts at: 4439 4439
Size: 6856
EarI CTCTTCn'nnn_
Cuts at: 1358 2320 2527 2636 3014 3379 3559 4877 5087
Size: 962 207 109 378 365 180 1318 210
Cuts at: 5087 1358
Size: 3127
Fragments arranged by size:
3127 1318 962 378 365 210 207 180
109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2745 5062 5374 5520 6666 2745
Size: 2317 312 146 1146 2935
Fragments arranged by size:
2935 2317 1146 312 146
EcoICRI GAG'CTC
Cuts at: 132 3372 3540 3777 3866 132
Size: 3240 168 237 89 3122
Fragments arranged by size:
3240 3122 237 168 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 599 608 776 1382 1387 1631 2012 2285
Size: 386 9 168 606 5 244 381 273
Cuts at: 2285 2503 2698 2995 3069 3124 3126 3247 3494
Size: 218 195 297 74 55 2 121 247
Cuts at: 3494 4679 5111 5860 6128 213
Size: 1185 432 749 268 941
Fragments arranged by size:
1185 941 749 606 432 386 381 297
273 268 247 244 218 195 168 121
74 55 9 5 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2137 2137
Size: 6856
EcoO109I rG'GnC_Cy
Cuts at: 630 631 1203 2864 3425 3993 630
Size: 1 572 1661 561 568 3493
Fragments arranged by size:
3493 1661 572 568 561 1
EcoRI G'AATT_C
Cuts at: 690 1267 3858 690
Size: 577 2591 3688
Fragments arranged by size:
3688 2591 577
EcoRV GAT'ATC
Cuts at: 1679 1679
Size: 6856
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 408 2346 3390 4873 408
Size: 1938 1044 1483 2391
Fragments arranged by size:
2391 1938 1483 1044
FspI TGC'GCA
Cuts at: 4635 4635
Size: 6856
HaeII r_GCGC'y
Cuts at: 2397 4288 4536 5560 6539 2397
Size: 1891 248 1024 979 2714
Fragments arranged by size:
2714 1891 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 286 318 1515 1547 4696 4726 4728 4758 286
Size: 32 1197 32 3149 30 2 30 2384
Fragments arranged by size:
3149 2384 1197 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3893 3893
Size: 6856
HindIII A'AGCT_T
Cuts at: 241 758 1633 1994 2355 241
Size: 517 875 361 361 4742
Fragments arranged by size:
4742 875 517 361 361
KasI G'GCGC_C
Cuts at: 4284 4532 4284
Size: 248 6608
KpnI G_GTAC'C
Cuts at: 221 3195 3874 221
Size: 2974 679 3203
Fragments arranged by size:
3203 2974 679
MfeI C'AATT_G
Cuts at: 3175 3175
Size: 6856
MluI A'CGCG_T
Cuts at: 6289 6289
Size: 6856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 329 1512 1917 3108 5527 5711 329
Size: 1183 405 1191 2419 184 1474
Fragments arranged by size:
2419 1474 1191 1183 405 184
MscI TGG'CCA
Cuts at: 4615 4615
Size: 6856
MslI CAynn'nnrTG
Cuts at: 2618 3032 3264 3353 3438 4843 4970 2618
Size: 414 232 89 85 1405 127 4504
Fragments arranged by size:
4504 1405 414 232 127 89 85
MspA1I CmG'CkG
Cuts at: 153 165 736 754 846 1227 2481 3146 3965
Size: 12 571 18 92 381 1254 665 819
Cuts at: 3965 4279 4639 5654 5899 153
Size: 314 360 1015 245 1110
Fragments arranged by size:
1254 1110 1015 819 665 571 381 360
314 245 92 18 12
NaeI GCC'GGC
Cuts at: 148 5035 148
Size: 4887 1969
NarI GG'CG_CC
Cuts at: 4285 4533 4285
Size: 248 6608
NcoI C'CATG_G
Cuts at: 125 2024 2490 125
Size: 1899 466 4491
Fragments arranged by size:
4491 1899 466
NgoMIV G'CCGG_C
Cuts at: 146 5033 146
Size: 4887 1969
NruI TCG'CGA
Cuts at: 5217 5217
Size: 6856
NsiI A_TGCA'T
Cuts at: 315 2808 315
Size: 2493 4363
NspI r_CATG'y
Cuts at: 1843 2552 2592 2810 3334 3907 4407 5316 1843
Size: 709 40 218 524 573 500 909 3383
Fragments arranged by size:
3383 909 709 573 524 500 218 40
PciI A'CATG_T
Cuts at: 2548 5312 2548
Size: 2764 4092
PfoI T'CCnGG_A
Cuts at: 3471 3471
Size: 6856
PmeI GTTT'AAAC
Cuts at: 3853 3853
Size: 6856
PmlI CAC'GTG
Cuts at: 2859 3071 2859
Size: 212 6644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 175 207 5995 6027 175
Size: 32 5788 32 1004
Fragments arranged by size:
5788 1004 32 32
PpuMI rG'GwC_Cy
Cuts at: 1203 2864 3425 1203
Size: 1661 561 4634
Fragments arranged by size:
4634 1661 561
PspOMI G'GGCC_C
Cuts at: 630 630
Size: 6856
PstI C_TGCA'G
Cuts at: 161 1092 2099 2896 3398 3901 4586 161
Size: 931 1007 797 502 503 685 2431
Fragments arranged by size:
2431 1007 931 797 685 503 502
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6856
PvuII CAG'CTG
Cuts at: 153 165 754 846 1227 2481 3146 4279 4639
Size: 12 589 92 381 1254 665 1133 360
Cuts at: 4639 153
Size: 2370
Fragments arranged by size:
2370 1254 1133 665 589 381 360 92
12
RsrII CG'GwC_CG
Cuts at: 5049 5049
Size: 6856
SacI G_AGCT'C
Cuts at: 134 3374 3542 3779 3868 134
Size: 3240 168 237 89 3122
Fragments arranged by size:
3240 3122 237 168 89
SalI G'TCGA_C
Cuts at: 3891 3891
Size: 6856
SanDI GG'GwC_CC
Cuts at: 3425 3425
Size: 6856
SapI GCTCTTCn'nnn_
Cuts at: 2320 3379 4877 5087 2320
Size: 1059 1498 210 4089
Fragments arranged by size:
4089 1498 1059 210
SbfI CC_TGCA'GG
Cuts at: 3901 3901
Size: 6856
ScaI AGT'ACT
Cuts at: 2815 6373 2815
Size: 3558 3298
SfcI C'TryA_G
Cuts at: 33 141 157 424 749 1088 2095 2892 3394
Size: 108 16 267 325 339 1007 797 502
Cuts at: 3394 3897 4582 5577 5768 33
Size: 503 685 995 191 1121
Fragments arranged by size:
1121 1007 995 797 685 503 502 339
325 267 191 108 16
SfoI GGC'GCC
Cuts at: 4286 4534 4286
Size: 248 6608
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6856
SmaI CCC'GGG
Cuts at: 3876 3876
Size: 6856
SmlI C'TyrA_G
Cuts at: 557 4009 4924 5418 5680 557
Size: 3452 915 494 262 1733
Fragments arranged by size:
3452 1733 915 494 262
SnaBI TAC'GTA
Cuts at: 3846 3846
Size: 6856
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6856
SphI G_CATG'C
Cuts at: 2592 2810 3907 4407 2592
Size: 218 1097 500 5041
Fragments arranged by size:
5041 1097 500 218
SspI AAT'ATT
Cuts at: 1078 1078
Size: 6856
StuI AGG'CCT
Cuts at: 2093 2344 2752 3166 2093
Size: 251 408 414 5783
Fragments arranged by size:
5783 414 408 251
StyI C'CwwG_G
Cuts at: 125 1259 1325 2024 2447 2490 3988 4342 125
Size: 1134 66 699 423 43 1498 354 2639
Fragments arranged by size:
2639 1498 1134 699 423 354 66 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1338 2761 3614 4437 4570 5029 5240 1338
Size: 1423 853 823 133 459 211 2954
Fragments arranged by size:
2954 1423 853 823 459 211 133
TatI w'GTAC_w
Cuts at: 167 359 2813 3446 6371 167
Size: 192 2454 633 2925 652
Fragments arranged by size:
2925 2454 652 633 192
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 379 411 3229 3261 6373 6405 379
Size: 32 2818 32 3112 32 830
Fragments arranged by size:
3112 2818 830 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1173 4651 1173
Size: 3478 3378
XbaI T'CTAG_A
Cuts at: 858 1689 3885 858
Size: 831 2196 3829
Fragments arranged by size:
3829 2196 831
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2497 3178 4349 2497
Size: 681 1171 5004
Fragments arranged by size:
5004 1171 681
XmaI C'CCGG_G
Cuts at: 3874 3874
Size: 6856
XmnI GAAnn'nnTTC
Cuts at: 970 2329 2629 3082 3139 970
Size: 1359 300 453 57 4687
Fragments arranged by size:
4687 1359 453 300 57
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NsiI NspI PciI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BaeI BglI BmgBI
BmtI BsiWI BssHII BstXI BstZ17I CspCI FseI FspAI
HpaI NdeI NheI NotI PacI PasI PflMI PshAI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SrfI
SwaI XhoI ZraI