Restriction map for
pF1KSDA0479
(Circular) (Six-base) MAPSORT of: pF1KSDA0479.seq Check: 7535 from: 1 to: 4060
pF1KSDA0479 4060 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1096 1096
Size: 4060
Acc65I G'GTAC_C
Cuts at: 1074 1074
Size: 4060
AclI AA'CG_TT
Cuts at: 3900 3900
Size: 4060
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1883 2315 3064 3332 1883
Size: 432 749 268 2611
Fragments arranged by size:
2611 749 432 268
AflIII A'CryG_T
Cuts at: 1410 2516 3493 1410
Size: 1106 977 1977
Fragments arranged by size:
1977 1106 977
AgeI A'CCGG_T
Cuts at: 2448 2448
Size: 4060
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 697 697
Size: 4060
AlwNI CAG_nnn'CTG
Cuts at: 1363 2932 1363
Size: 1569 2491
ApaLI G'TGCA_C
Cuts at: 2830 2830
Size: 4060
ApoI r'AATT_y
Cuts at: 351 1062 3413 3669 351
Size: 711 2351 256 742
Fragments arranged by size:
2351 742 711 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4060
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4060
AvaI C'yCGr_G
Cuts at: 818 1078 818
Size: 260 3800
BamHI G'GATC_C
Cuts at: 707 1083 707
Size: 376 3684
BanI G'GyrC_C
Cuts at: 317 813 1074 1488 1736 1771 317
Size: 496 261 414 248 35 2606
Fragments arranged by size:
2606 496 414 261 248 35
BanII G_rGCy'C
Cuts at: 761 1072 2102 761
Size: 311 1030 2719
Fragments arranged by size:
2719 1030 311
BbeI G_GCGC'C
Cuts at: 1492 1740 1492
Size: 248 3812
BbvCI CC'TCA_GC
Cuts at: 604 604
Size: 4060
BciVI GTATCCnnnnn_n'
Cuts at: 1956 2719 4060 1956
Size: 763 1341 1956
Fragments arranged by size:
1956 1341 763
BclI T'GATC_A
Cuts at: 1578 1578
Size: 4060
BglII A'GATC_T
Cuts at: 578 1573 3612 578
Size: 995 2039 1026
Fragments arranged by size:
2039 1026 995
BlpI GC'TnA_GC
Cuts at: 1170 1170
Size: 4060
Bme1580I G_kGCm'C
Cuts at: 197 676 818 1683 1776 2834 197
Size: 479 142 865 93 1058 1423
Fragments arranged by size:
1423 1058 865 479 142 93
BmrI ACTGGGnnnn_n'
Cuts at: 1270 1351 1686 3832 1270
Size: 81 335 2146 1498
Fragments arranged by size:
2146 1498 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 619 2425 619
Size: 1806 2254
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 757
Size: 4060
Bpu10I CC'TnA_GC
Cuts at: 604 604
Size: 4060
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1234 2113 2607 2905 1234
Size: 879 494 298 2389
Fragments arranged by size:
2389 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 127 514 3764 127
Size: 387 3250 423
Fragments arranged by size:
3250 423 387
BsaAI yAC'GTr
Cuts at: 1050 1411 1050
Size: 361 3699
BsaBI GATnn'nnATC
Cuts at: 6 1572 3611 6
Size: 1566 2039 455
Fragments arranged by size:
2039 1566 455
BsaHI Gr'CG_yC
Cuts at: 1489 1737 2441 3937 1489
Size: 248 704 1496 1612
Fragments arranged by size:
1612 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1247 1768 2448 2722 2869 3280 1247
Size: 521 680 274 147 411 2027
Fragments arranged by size:
2027 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 846 846
Size: 4060
BseYI C'CCAG_C
Cuts at: 527 992 1483 2241 2820 3316 527
Size: 465 491 758 579 496 1271
Fragments arranged by size:
1271 758 579 496 491 465
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 214
Size: 4060
BsiEI CG_ry'CG
Cuts at: 104 1600 1646 2856 104
Size: 1496 46 1210 1308
Fragments arranged by size:
1496 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1072 1850 2040 2834 1072
Size: 778 190 794 2298
Fragments arranged by size:
2298 794 778 190
BsmI GAATG_Cn'
Cuts at: 666 666
Size: 4060
BsmBI CGTCTCn'nnnn_
Cuts at: 328 328
Size: 4060
Bsp1286I G_dGCh'C
Cuts at: 197 676 761 818 1072 1683 1776 1850 2040
Size: 479 85 57 254 611 93 74 190
Cuts at: 2040 2102 2834 197
Size: 62 732 1423
Fragments arranged by size:
1423 732 611 479 254 190 93 85
74 62 57
BspEI T'CCGG_A
Cuts at: 3280 3280
Size: 4060
BspHI T'CATG_A
Cuts at: 440 845 3236 440
Size: 405 2391 1264
Fragments arranged by size:
2391 1264 405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 418 740 1108 1322 1624 2005 418
Size: 322 368 214 302 381 2473
Fragments arranged by size:
2473 381 368 322 302 214
BsrBI CCG'CTC
Cuts at: 50 2350 2404 3890 4055 50
Size: 2300 54 1486 165 55
Fragments arranged by size:
2300 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1970 1970
Size: 4060
BsrFI r'CCGG_y
Cuts at: 314 630 996 2056 2237 2448 314
Size: 316 366 1060 181 211 1926
Fragments arranged by size:
1926 1060 366 316 211 181
BsrGI T'GTAC_A
Cuts at: 1028 1028
Size: 4060
BssSI C'ACGA_G
Cuts at: 304 2329 2689 304
Size: 2025 360 1675
Fragments arranged by size:
2025 1675 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1435 1435
Size: 4060
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4060
BstXI CCAn_nnnn'nTGG
Cuts at: 582 761 582
Size: 179 3881
BstYI r'GATC_y
Cuts at: 1 578 611 707 1083 1550 1573 1908 2154
Size: 577 33 96 376 467 23 335 246
Cuts at: 2154 3168 3254 3266 3612 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 577 467 449 376 346 335 246
96 86 33 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2008 2189 2352 2008
Size: 181 163 3716
Fragments arranged by size:
3716 181 163
BtsI GCAGTG_nn'
Cuts at: 566 1301 566
Size: 735 3325
DraI TTT'AAA
Cuts at: 1057 1057
Size: 4060
DrdI GACnn_nn'nnGTC
Cuts at: 1764 2624 1764
Size: 860 3200
EaeI y'GGCC_r
Cuts at: 341 554 969 1643 1817 2208 2235 3582 341
Size: 213 415 674 174 391 27 1347 819
Fragments arranged by size:
1347 819 674 415 391 213 174 27
EagI C'GGCC_G
Cuts at: 1643 1643
Size: 4060
EarI CTCTTCn'nnn_
Cuts at: 439 686 2081 2291 439
Size: 247 1395 210 2208
Fragments arranged by size:
2208 1395 247 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 613 2266 2578 2724 3870 613
Size: 1653 312 146 1146 803
Fragments arranged by size:
1653 1146 803 312 146
EcoICRI GAG'CTC
Cuts at: 1070 1070
Size: 4060
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 1883 2315 3064 3332 757
Size: 1126 432 749 268 1485
Fragments arranged by size:
1485 1126 749 432 268
EcoO109I rG'GnC_Cy
Cuts at: 300 435 1197 300
Size: 135 762 3163
Fragments arranged by size:
3163 762 135
EcoRI G'AATT_C
Cuts at: 351 1062 351
Size: 711 3349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 4060
FspI TGC'GCA
Cuts at: 1839 1839
Size: 4060
HaeII r_GCGC'y
Cuts at: 1492 1740 2764 3743 1492
Size: 248 1024 979 1809
Fragments arranged by size:
1809 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 973 1005 1900 1930 1932 1962 973
Size: 32 895 30 2 30 3071
Fragments arranged by size:
3071 895 32 30 30 2
HincII GTy'rAC
Cuts at: 477 1097 477
Size: 620 3440
KasI G'GCGC_C
Cuts at: 1488 1736 1488
Size: 248 3812
KpnI G_GTAC'C
Cuts at: 1078 1078
Size: 4060
MluI A'CGCG_T
Cuts at: 3493 3493
Size: 4060
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 401 2731 2915 401
Size: 2330 184 1546
Fragments arranged by size:
2330 1546 184
MscI TGG'CCA
Cuts at: 556 1819 556
Size: 1263 2797
MslI CAynn'nnrTG
Cuts at: 202 679 889 892 2047 2174 202
Size: 477 210 3 1155 127 2088
Fragments arranged by size:
2088 1155 477 210 127 3
MspA1I CmG'CkG
Cuts at: 339 1026 1169 1483 1843 2858 3103 339
Size: 687 143 314 360 1015 245 1296
Fragments arranged by size:
1296 1015 687 360 314 245 143
NaeI GCC'GGC
Cuts at: 316 2239 316
Size: 1923 2137
NarI GG'CG_CC
Cuts at: 1489 1737 1489
Size: 248 3812
NgoMIV G'CCGG_C
Cuts at: 314 2237 314
Size: 1923 2137
NruI TCG'CGA
Cuts at: 2421 2421
Size: 4060
NspI r_CATG'y
Cuts at: 1111 1611 2520 1111
Size: 500 909 2651
Fragments arranged by size:
2651 909 500
PasI CC'CwG_GG
Cuts at: 754 754
Size: 4060
PciI A'CATG_T
Cuts at: 2516 2516
Size: 4060
PflMI CCAn_nnn'nTGG
Cuts at: 978 978
Size: 4060
PmeI GTTT'AAAC
Cuts at: 1057 1057
Size: 4060
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3199 3231 3199
Size: 32 4028
PpuMI rG'GwC_Cy
Cuts at: 435 435
Size: 4060
PstI C_TGCA'G
Cuts at: 415 573 959 1105 1790 415
Size: 158 386 146 685 2685
Fragments arranged by size:
2685 685 386 158 146
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4060
PvuII CAG'CTG
Cuts at: 339 1026 1483 1843 339
Size: 687 457 360 2556
Fragments arranged by size:
2556 687 457 360
RsrII CG'GwC_CG
Cuts at: 2253 2253
Size: 4060
SacI G_AGCT'C
Cuts at: 1072 1072
Size: 4060
SalI G'TCGA_C
Cuts at: 1095 1095
Size: 4060
SapI GCTCTTCn'nnn_
Cuts at: 2081 2291 2081
Size: 210 3850
SbfI CC_TGCA'GG
Cuts at: 1105 1105
Size: 4060
ScaI AGT'ACT
Cuts at: 3577 3577
Size: 4060
SfcI C'TryA_G
Cuts at: 33 411 569 955 1101 1786 2781 2972 33
Size: 378 158 386 146 685 995 191 1121
Fragments arranged by size:
1121 995 685 386 378 191 158 146
SfoI GGC'GCC
Cuts at: 1490 1738 1490
Size: 248 3812
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4060
SgrAI Cr'CCGG_yG
Cuts at: 630 630
Size: 4060
SmaI CCC'GGG
Cuts at: 820 1080 820
Size: 260 3800
SmlI C'TyrA_G
Cuts at: 1213 2128 2622 2884 1213
Size: 915 494 262 2389
Fragments arranged by size:
2389 915 494 262
SnaBI TAC'GTA
Cuts at: 1050 1050
Size: 4060
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4060
SphI G_CATG'C
Cuts at: 1111 1611 1111
Size: 500 3560
StyI C'CwwG_G
Cuts at: 376 1192 1546 376
Size: 816 354 2890
Fragments arranged by size:
2890 816 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 145 854 1641 1774 2233 2444 145
Size: 709 787 133 459 211 1761
Fragments arranged by size:
1761 787 709 459 211 133
TatI w'GTAC_w
Cuts at: 1028 3575 1028
Size: 2547 1513
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 603 635 3577 3609 603
Size: 32 2942 32 1054
Fragments arranged by size:
2942 1054 32 32
Tth111I GACn'n_nGTC
Cuts at: 1855 1855
Size: 4060
XbaI T'CTAG_A
Cuts at: 1089 1089
Size: 4060
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 286 945 1553 286
Size: 659 608 2793
Fragments arranged by size:
2793 659 608
XmaI C'CCGG_G
Cuts at: 818 1078 818
Size: 260 3800
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NruI NspI PasI PciI PflMI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NsiI PacI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SrfI SspI StuI SwaI XhoI
XmnI ZraI