Restriction map for
pF1KSDA0513
(Circular) (Six-base) MAPSORT of: pF1KSDA0513.seq Check: 538 from: 1 to: 4372
pF1KSDA0513 4372 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 382 1408 382
Size: 1026 3346
Acc65I G'GTAC_C
Cuts at: 1386 1386
Size: 4372
AclI AA'CG_TT
Cuts at: 4212 4212
Size: 4372
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 901 1030 1297 2195 2627 3376 3644 838
Size: 63 129 267 898 432 749 268 1566
Fragments arranged by size:
1566 898 749 432 268 267 129 63
AfeI AGC'GCT
Cuts at: 556 556
Size: 4372
AflIII A'CryG_T
Cuts at: 1722 2828 3805 1722
Size: 1106 977 2289
Fragments arranged by size:
2289 1106 977
AgeI A'CCGG_T
Cuts at: 2760 2760
Size: 4372
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 626 626
Size: 4372
AlwNI CAG_nnn'CTG
Cuts at: 714 1675 3244 714
Size: 961 1569 1842
Fragments arranged by size:
1842 1569 961
ApaI G_GGCC'C
Cuts at: 890 890
Size: 4372
ApaLI G'TGCA_C
Cuts at: 3142 3142
Size: 4372
ApoI r'AATT_y
Cuts at: 1374 3725 3981 1374
Size: 2351 256 1765
Fragments arranged by size:
2351 1765 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4372
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4372
AvaI C'yCGr_G
Cuts at: 728 964 1390 728
Size: 236 426 3710
Fragments arranged by size:
3710 426 236
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 531 564 531
Size: 33 4339
BamHI G'GATC_C
Cuts at: 1395 1395
Size: 4372
BanI G'GyrC_C
Cuts at: 177 201 1145 1386 1800 2048 2083 177
Size: 24 944 241 414 248 35 2466
Fragments arranged by size:
2466 944 414 248 241 35 24
BanII G_rGCy'C
Cuts at: 154 890 1384 2414 154
Size: 736 494 1030 2112
Fragments arranged by size:
2112 1030 736 494
BbeI G_GCGC'C
Cuts at: 1804 2052 1804
Size: 248 4124
BbsI GAAGACnn'nnnn_
Cuts at: 917 917
Size: 4372
BciVI GTATCCnnnnn_n'
Cuts at: 2268 3031 4372 2268
Size: 763 1341 2268
Fragments arranged by size:
2268 1341 763
BclI T'GATC_A
Cuts at: 1890 1890
Size: 4372
BglI GCCn_nnn'nGGC
Cuts at: 1026 1026
Size: 4372
BglII A'GATC_T
Cuts at: 440 989 1885 3924 440
Size: 549 896 2039 888
Fragments arranged by size:
2039 896 888 549
BlpI GC'TnA_GC
Cuts at: 1482 1482
Size: 4372
Bme1580I G_kGCm'C
Cuts at: 552 890 1219 1995 2088 3146 552
Size: 338 329 776 93 1058 1778
Fragments arranged by size:
1778 1058 776 338 329 93
BmrI ACTGGGnnnn_n'
Cuts at: 1582 1663 1998 4144 1582
Size: 81 335 2146 1810
Fragments arranged by size:
2146 1810 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 324 375 2737 324
Size: 51 2362 1959
Fragments arranged by size:
2362 1959 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 470 889 1136 217
Size: 253 419 247 3453
Fragments arranged by size:
3453 419 253 247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1546 2425 2919 3217 1546
Size: 879 494 298 2701
Fragments arranged by size:
2701 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 124 256 391 865 4076 124
Size: 132 135 474 3211 420
Fragments arranged by size:
3211 474 420 135 132
BsaAI yAC'GTr
Cuts at: 432 543 1362 1723 432
Size: 111 819 361 3081
Fragments arranged by size:
3081 819 361 111
BsaBI GATnn'nnATC
Cuts at: 6 1884 3923 6
Size: 1878 2039 455
Fragments arranged by size:
2039 1878 455
BsaHI Gr'CG_yC
Cuts at: 1801 2049 2753 4249 1801
Size: 248 704 1496 1924
Fragments arranged by size:
1924 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1181 1559 2080 2760 3034 3181 3592 1181
Size: 378 521 680 274 147 411 1961
Fragments arranged by size:
1961 680 521 411 378 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 174 204 1129 1159 174
Size: 30 925 30 3387
Fragments arranged by size:
3387 925 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 333 351 487 1135 1138 1141 1177 333
Size: 18 136 648 3 3 36 3528
Fragments arranged by size:
3528 648 136 36 18 3 3
BseYI C'CCAG_C
Cuts at: 551 1212 1795 2553 3132 3628 551
Size: 661 583 758 579 496 1295
Fragments arranged by size:
1295 758 661 583 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 622
Size: 4372
BsiEI CG_ry'CG
Cuts at: 104 1912 1958 3168 104
Size: 1808 46 1210 1308
Fragments arranged by size:
1808 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 376 1384 2162 2352 3146 376
Size: 1008 778 190 794 1602
Fragments arranged by size:
1602 1008 794 778 190
BsmI GAATG_Cn'
Cuts at: 1053 1053
Size: 4372
Bsp1286I G_dGCh'C
Cuts at: 154 376 552 890 1219 1384 1995 2088 2162
Size: 222 176 338 329 165 611 93 74
Cuts at: 2162 2352 2414 3146 154
Size: 190 62 732 1380
Fragments arranged by size:
1380 732 611 338 329 222 190 176
165 93 74 62
BspEI T'CCGG_A
Cuts at: 3592 3592
Size: 4372
BspHI T'CATG_A
Cuts at: 668 3548 668
Size: 2880 1492
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1420 1634 1936 2317 1420
Size: 214 302 381 3475
Fragments arranged by size:
3475 381 302 214
BsrBI CCG'CTC
Cuts at: 50 810 2662 2716 4202 4367 50
Size: 760 1852 54 1486 165 55
Fragments arranged by size:
1852 1486 760 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1272 2282 1272
Size: 1010 3362
BsrFI r'CCGG_y
Cuts at: 2368 2549 2760 2368
Size: 181 211 3980
Fragments arranged by size:
3980 211 181
BsrGI T'GTAC_A
Cuts at: 998 998
Size: 4372
BssSI C'ACGA_G
Cuts at: 2641 3001 2641
Size: 360 4012
BstAPI GCAn_nnn'nTGC
Cuts at: 1233 1747 1233
Size: 514 3858
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4372
BstYI r'GATC_y
Cuts at: 1 440 989 1395 1862 1885 2220 2466 3480
Size: 439 549 406 467 23 335 246 1014
Cuts at: 3480 3566 3578 3924 1
Size: 86 12 346 449
Fragments arranged by size:
1014 549 467 449 439 406 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 173 173
Size: 4372
BtgI C'CryG_G
Cuts at: 125 146 740 125
Size: 21 594 3757
Fragments arranged by size:
3757 594 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 242 786 2320 2501 2664 242
Size: 544 1534 181 163 1950
Fragments arranged by size:
1950 1534 544 181 163
BtsI GCAGTG_nn'
Cuts at: 510 620 1613 510
Size: 110 993 3269
Fragments arranged by size:
3269 993 110
DraI TTT'AAA
Cuts at: 1369 1369
Size: 4372
DrdI GACnn_nn'nnGTC
Cuts at: 270 2076 2936 270
Size: 1806 860 1706
Fragments arranged by size:
1806 1706 860
EaeI y'GGCC_r
Cuts at: 785 830 893 1349 1955 2129 2520 2547 3894
Size: 45 63 456 606 174 391 27 1347
Cuts at: 3894 785
Size: 1263
Fragments arranged by size:
1347 1263 606 456 391 174 63 45
27
EagI C'GGCC_G
Cuts at: 1955 1955
Size: 4372
EarI CTCTTCn'nnn_
Cuts at: 192 389 1126 1304 2393 2603 192
Size: 197 737 178 1089 210 1961
Fragments arranged by size:
1961 1089 737 210 197 178
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2578 2890 3036 4182 2578
Size: 312 146 1146 2768
Fragments arranged by size:
2768 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1382 1382
Size: 4372
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 470 838 889 901 1030 1136 1297 2195
Size: 253 368 51 12 129 106 161 898
Cuts at: 2195 2627 3376 3644 217
Size: 432 749 268 945
Fragments arranged by size:
945 898 749 432 368 268 253 161
129 106 51 12
EcoNI CCTnn'n_nnAGG
Cuts at: 831 831
Size: 4372
EcoO109I rG'GnC_Cy
Cuts at: 141 886 887 1509 141
Size: 745 1 622 3004
Fragments arranged by size:
3004 745 622 1
EcoRI G'AATT_C
Cuts at: 1374 1374
Size: 4372
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 873 2389 873
Size: 1516 2856
FspI TGC'GCA
Cuts at: 2151 2151
Size: 4372
HaeII r_GCGC'y
Cuts at: 558 1804 2052 3076 4055 558
Size: 1246 248 1024 979 875
Fragments arranged by size:
1246 1024 979 875 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 235 267 2212 2242 2244 2274 235
Size: 32 1945 30 2 30 2333
Fragments arranged by size:
2333 1945 32 30 30 2
HincII GTy'rAC
Cuts at: 1409 1409
Size: 4372
KasI G'GCGC_C
Cuts at: 1800 2048 1800
Size: 248 4124
KpnI G_GTAC'C
Cuts at: 1390 1390
Size: 4372
MluI A'CGCG_T
Cuts at: 3805 3805
Size: 4372
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 371 389 3043 3227 371
Size: 18 2654 184 1516
Fragments arranged by size:
2654 1516 184 18
MscI TGG'CCA
Cuts at: 895 1351 2131 895
Size: 456 780 3136
Fragments arranged by size:
3136 780 456
MslI CAynn'nnrTG
Cuts at: 673 2359 2486 673
Size: 1686 127 2559
Fragments arranged by size:
2559 1686 127
MspA1I CmG'CkG
Cuts at: 711 742 779 1212 1481 1795 2155 3170 3415
Size: 31 37 433 269 314 360 1015 245
Cuts at: 3415 711
Size: 1668
Fragments arranged by size:
1668 1015 433 360 314 269 245 37
31
NaeI GCC'GGC
Cuts at: 2551 2551
Size: 4372
NarI GG'CG_CC
Cuts at: 1801 2049 1801
Size: 248 4124
NcoI C'CATG_G
Cuts at: 125 125
Size: 4372
NgoMIV G'CCGG_C
Cuts at: 2549 2549
Size: 4372
NruI TCG'CGA
Cuts at: 2733 2733
Size: 4372
NspI r_CATG'y
Cuts at: 1236 1423 1923 2832 1236
Size: 187 500 909 2776
Fragments arranged by size:
2776 909 500 187
PasI CC'CwG_GG
Cuts at: 237 941 237
Size: 704 3668
PciI A'CATG_T
Cuts at: 2828 2828
Size: 4372
PmeI GTTT'AAAC
Cuts at: 1369 1369
Size: 4372
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3511 3543 3511
Size: 32 4340
PpuMI rG'GwC_Cy
Cuts at: 141 141
Size: 4372
PspOMI G'GGCC_C
Cuts at: 886 886
Size: 4372
PstI C_TGCA'G
Cuts at: 221 502 1417 2102 221
Size: 281 915 685 2491
Fragments arranged by size:
2491 915 685 281
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4372
PvuII CAG'CTG
Cuts at: 711 779 1212 1795 2155 711
Size: 68 433 583 360 2928
Fragments arranged by size:
2928 583 433 360 68
RsrII CG'GwC_CG
Cuts at: 2565 2565
Size: 4372
SacI G_AGCT'C
Cuts at: 1384 1384
Size: 4372
SacII CC_GC'GG
Cuts at: 743 743
Size: 4372
SalI G'TCGA_C
Cuts at: 1407 1407
Size: 4372
SapI GCTCTTCn'nnn_
Cuts at: 1304 2393 2603 1304
Size: 1089 210 3073
Fragments arranged by size:
3073 1089 210
SbfI CC_TGCA'GG
Cuts at: 1417 1417
Size: 4372
ScaI AGT'ACT
Cuts at: 496 3889 496
Size: 3393 979
SfcI C'TryA_G
Cuts at: 33 217 498 1413 2098 3093 3284 33
Size: 184 281 915 685 995 191 1121
Fragments arranged by size:
1121 995 915 685 281 191 184
SfoI GGC'GCC
Cuts at: 1802 2050 1802
Size: 248 4124
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4372
SmaI CCC'GGG
Cuts at: 730 1392 730
Size: 662 3710
SmlI C'TyrA_G
Cuts at: 1525 2440 2934 3196 1525
Size: 915 494 262 2701
Fragments arranged by size:
2701 915 494 262
SnaBI TAC'GTA
Cuts at: 1362 1362
Size: 4372
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4372
SphI G_CATG'C
Cuts at: 1423 1923 1423
Size: 500 3872
StyI C'CwwG_G
Cuts at: 125 1504 1858 125
Size: 1379 354 2639
Fragments arranged by size:
2639 1379 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 195 1953 2086 2545 2756 195
Size: 1758 133 459 211 1811
Fragments arranged by size:
1811 1758 459 211 133
TatI w'GTAC_w
Cuts at: 494 998 3887 494
Size: 504 2889 979
Fragments arranged by size:
2889 979 504
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 174 206 1129 1161 3889 3921 174
Size: 32 923 32 2728 32 625
Fragments arranged by size:
2728 923 625 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1066 2167 1066
Size: 1101 3271
XbaI T'CTAG_A
Cuts at: 1401 1401
Size: 4372
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1865 1865
Size: 4372
XmaI C'CCGG_G
Cuts at: 728 1390 728
Size: 662 3710
XmnI GAAnn'nnTTC
Cuts at: 442 442
Size: 4372
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsmBI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SrfI SspI StuI
SwaI XhoI ZraI