Restriction map for
pF1KSDA0517
(Circular) (Six-base) MAPSORT of: pF1KSDA0517.seq Check: 8875 from: 1 to: 5452
pF1KSDA0517 5452 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 2488 2488
Size: 5452
Acc65I G'GTAC_C
Cuts at: 1636 2466 1636
Size: 830 4622
AclI AA'CG_TT
Cuts at: 5292 5292
Size: 5452
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 1150 1657 2357 3275 3707 4456 4724 739
Size: 411 507 700 918 432 749 268 1467
Fragments arranged by size:
1467 918 749 700 507 432 411 268
AflIII A'CryG_T
Cuts at: 2802 3908 4885 2802
Size: 1106 977 3369
Fragments arranged by size:
3369 1106 977
AgeI A'CCGG_T
Cuts at: 3840 3840
Size: 5452
AleI CACnn'nnGTG
Cuts at: 134 532 1819 134
Size: 398 1287 3767
Fragments arranged by size:
3767 1287 398
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 179 883 2231 179
Size: 704 1348 3400
Fragments arranged by size:
3400 1348 704
AlwNI CAG_nnn'CTG
Cuts at: 267 529 2401 2755 4324 267
Size: 262 1872 354 1569 1395
Fragments arranged by size:
1872 1569 1395 354 262
ApaLI G'TGCA_C
Cuts at: 627 4222 627
Size: 3595 1857
ApoI r'AATT_y
Cuts at: 586 1900 2167 2454 4805 5061 586
Size: 1314 267 287 2351 256 977
Fragments arranged by size:
2351 1314 977 287 267 256
AseI AT'TA_AT
Cuts at: 20 1708 20
Size: 1688 3764
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5452
AvaI C'yCGr_G
Cuts at: 415 1150 1527 2470 415
Size: 735 377 943 3397
Fragments arranged by size:
3397 943 735 377
BamHI G'GATC_C
Cuts at: 2282 2475 2282
Size: 193 5259
BanI G'GyrC_C
Cuts at: 220 341 1366 1636 2466 2880 3128 3163 220
Size: 121 1025 270 830 414 248 35 2509
Fragments arranged by size:
2509 1025 830 414 270 248 121 35
BanII G_rGCy'C
Cuts at: 839 2464 3494 839
Size: 1625 1030 2797
Fragments arranged by size:
2797 1625 1030
BbeI G_GCGC'C
Cuts at: 2884 3132 2884
Size: 248 5204
BbsI GAAGACnn'nnnn_
Cuts at: 500 850 1619 500
Size: 350 769 4333
Fragments arranged by size:
4333 769 350
BbvCI CC'TCA_GC
Cuts at: 500 500
Size: 5452
BciVI GTATCCnnnnn_n'
Cuts at: 924 3348 4111 5452 924
Size: 2424 763 1341 924
Fragments arranged by size:
2424 1341 924 763
BclI T'GATC_A
Cuts at: 2970 2970
Size: 5452
BglI GCCn_nnn'nGGC
Cuts at: 2357 2357
Size: 5452
BglII A'GATC_T
Cuts at: 2965 5004 2965
Size: 2039 3413
BlpI GC'TnA_GC
Cuts at: 2562 2562
Size: 5452
Bme1580I G_kGCm'C
Cuts at: 631 1970 3075 3168 4226 631
Size: 1339 1105 93 1058 1857
Fragments arranged by size:
1857 1339 1105 1058 93
BmgBI CAC'GTC
Cuts at: 471 1542 471
Size: 1071 4381
BmrI ACTGGGnnnn_n'
Cuts at: 382 1829 2277 2662 2743 3078 5224 382
Size: 1447 448 385 81 335 2146 610
Fragments arranged by size:
2146 1447 610 448 385 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3817 3817
Size: 5452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 470 538 685 823 901 925 421
Size: 49 68 147 138 78 24 4948
Fragments arranged by size:
4948 147 138 78 68 49 24
Bpu10I CC'TnA_GC
Cuts at: 500 500
Size: 5452
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 2626 3505 3999 4297 652
Size: 1974 879 494 298 1807
Fragments arranged by size:
1974 1807 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1000 5156 1000
Size: 4156 1296
BsaAI yAC'GTr
Cuts at: 2442 2803 2442
Size: 361 5091
BsaBI GATnn'nnATC
Cuts at: 6 2964 5003 6
Size: 2958 2039 455
Fragments arranged by size:
2958 2039 455
BsaHI Gr'CG_yC
Cuts at: 2881 3129 3833 5329 2881
Size: 248 704 1496 3004
Fragments arranged by size:
3004 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2639 3160 3840 4114 4261 4672 2639
Size: 521 680 274 147 411 3419
Fragments arranged by size:
3419 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 126 156 1811 1841 1937 1967 126
Size: 30 1655 30 96 30 3611
Fragments arranged by size:
3611 1655 96 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 517 1878 517
Size: 1361 4091
BseYI C'CCAG_C
Cuts at: 2875 3633 4212 4708 2875
Size: 758 579 496 3619
Fragments arranged by size:
3619 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 307 1756 185
Size: 122 1449 3881
Fragments arranged by size:
3881 1449 122
BsiEI CG_ry'CG
Cuts at: 104 2992 3038 4248 104
Size: 2888 46 1210 1308
Fragments arranged by size:
2888 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 631 644 653 839 1318 2464 3242 3432 4226
Size: 13 9 186 479 1146 778 190 794
Cuts at: 4226 631
Size: 1857
Fragments arranged by size:
1857 1146 794 778 479 190 186 13
9
Bsp1286I G_dGCh'C
Cuts at: 631 644 653 839 1318 1970 2464 3075 3168
Size: 13 9 186 479 652 494 611 93
Cuts at: 3168 3242 3432 3494 4226 631
Size: 74 190 62 732 1857
Fragments arranged by size:
1857 732 652 611 494 479 190 186
93 74 62 13 9
BspEI T'CCGG_A
Cuts at: 4672 4672
Size: 5452
BspHI T'CATG_A
Cuts at: 4628 4628
Size: 5452
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2065 2500 2714 3016 3397 2065
Size: 435 214 302 381 4120
Fragments arranged by size:
4120 435 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1535 3742 3796 5282 5447 50
Size: 1485 2207 54 1486 165 55
Fragments arranged by size:
2207 1486 1485 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1850 1968 3362 1850
Size: 118 1394 3940
Fragments arranged by size:
3940 1394 118
BsrFI r'CCGG_y
Cuts at: 181 1285 3448 3629 3840 181
Size: 1104 2163 181 211 1793
Fragments arranged by size:
2163 1793 1104 211 181
BsrGI T'GTAC_A
Cuts at: 1382 1577 1827 1382
Size: 195 250 5007
Fragments arranged by size:
5007 250 195
BssSI C'ACGA_G
Cuts at: 3721 4081 3721
Size: 360 5092
BstAPI GCAn_nnn'nTGC
Cuts at: 281 2827 281
Size: 2546 2906
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5452
BstXI CCAn_nnnn'nTGG
Cuts at: 197 807 1821 197
Size: 610 1014 3828
Fragments arranged by size:
3828 1014 610
BstYI r'GATC_y
Cuts at: 1 1346 2282 2475 2942 2965 3300 3546 4560
Size: 1345 936 193 467 23 335 246 1014
Cuts at: 4560 4646 4658 5004 1
Size: 86 12 346 449
Fragments arranged by size:
1345 1014 936 467 449 346 335 246
193 86 23 12
BtgI C'CryG_G
Cuts at: 996 1084 1196 996
Size: 88 112 5252
Fragments arranged by size:
5252 112 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3400 3581 3744 3400
Size: 181 163 5108
Fragments arranged by size:
5108 181 163
BtsI GCAGTG_nn'
Cuts at: 527 2240 2693 527
Size: 1713 453 3286
Fragments arranged by size:
3286 1713 453
ClaI AT'CG_AT
Cuts at: 2427 2427
Size: 5452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 221 256 221
Size: 35 5417
DraI TTT'AAA
Cuts at: 851 2449 851
Size: 1598 3854
DrdI GACnn_nn'nnGTC
Cuts at: 3156 4016 3156
Size: 860 4592
EaeI y'GGCC_r
Cuts at: 138 209 428 1058 1193 1536 1755 2261 3035
Size: 71 219 630 135 343 219 506 774
Cuts at: 3035 3209 3600 3627 4974 138
Size: 174 391 27 1347 616
Fragments arranged by size:
1347 774 630 616 506 391 343 219
219 174 135 71 27
EagI C'GGCC_G
Cuts at: 3035 3035
Size: 5452
EarI CTCTTCn'nnn_
Cuts at: 758 1504 3473 3683 758
Size: 746 1969 210 2527
Fragments arranged by size:
2527 1969 746 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3658 3970 4116 5262 3658
Size: 312 146 1146 3848
Fragments arranged by size:
3848 1146 312 146
EcoICRI GAG'CTC
Cuts at: 837 2462 837
Size: 1625 3827
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 470 538 685 739 823 901 925 1150
Size: 49 68 147 54 84 78 24 225
Cuts at: 1150 1657 2357 3275 3707 4456 4724 421
Size: 507 700 918 432 749 268 1149
Fragments arranged by size:
1149 918 749 700 507 432 268 225
147 84 78 68 54 49 24
EcoO109I rG'GnC_Cy
Cuts at: 987 1014 1936 2077 2589 987
Size: 27 922 141 512 3850
Fragments arranged by size:
3850 922 512 141 27
EcoRI G'AATT_C
Cuts at: 2167 2454 2167
Size: 287 5165
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3469 3469
Size: 5452
FspI TGC'GCA
Cuts at: 859 3231 859
Size: 2372 3080
HaeII r_GCGC'y
Cuts at: 2884 3132 4156 5135 2884
Size: 248 1024 979 3201
Fragments arranged by size:
3201 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3292 3322 3324 3354 3292
Size: 30 2 30 5390
Fragments arranged by size:
5390 30 30 2
HincII GTy'rAC
Cuts at: 723 785 891 2489 723
Size: 62 106 1598 3686
Fragments arranged by size:
3686 1598 106 62
HpaI GTT'AAC
Cuts at: 785 785
Size: 5452
KasI G'GCGC_C
Cuts at: 2880 3128 2880
Size: 248 5204
KpnI G_GTAC'C
Cuts at: 1640 2470 1640
Size: 830 4622
MluI A'CGCG_T
Cuts at: 4885 4885
Size: 5452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2245 4123 4307 2245
Size: 1878 184 3390
Fragments arranged by size:
3390 1878 184
MscI TGG'CCA
Cuts at: 211 1195 1757 3211 211
Size: 984 562 1454 2452
Fragments arranged by size:
2452 1454 984 562
MslI CAynn'nnrTG
Cuts at: 134 195 532 1819 1835 2257 3439 3566 134
Size: 61 337 1287 16 422 1182 127 2020
Fragments arranged by size:
2020 1287 1182 422 337 127 61 16
MspA1I CmG'CkG
Cuts at: 711 927 1086 1101 1480 1803 1809 2092 2561
Size: 216 159 15 379 323 6 283 469
Cuts at: 2561 2875 3235 4250 4495 711
Size: 314 360 1015 245 1668
Fragments arranged by size:
1668 1015 469 379 360 323 314 283
245 216 159 15 6
NaeI GCC'GGC
Cuts at: 183 3631 183
Size: 3448 2004
NarI GG'CG_CC
Cuts at: 2881 3129 2881
Size: 248 5204
NcoI C'CATG_G
Cuts at: 996 996
Size: 5452
NgoMIV G'CCGG_C
Cuts at: 181 3629 181
Size: 3448 2004
NruI TCG'CGA
Cuts at: 3813 3813
Size: 5452
NspI r_CATG'y
Cuts at: 1578 2503 3003 3912 1578
Size: 925 500 909 3118
Fragments arranged by size:
3118 925 909 500
PciI A'CATG_T
Cuts at: 3908 3908
Size: 5452
PflMI CCAn_nnn'nTGG
Cuts at: 2347 2361 2347
Size: 14 5438
PfoI T'CCnGG_A
Cuts at: 515 1349 515
Size: 834 4618
PmeI GTTT'AAAC
Cuts at: 2449 2449
Size: 5452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4591 4623 4591
Size: 32 5420
PpuMI rG'GwC_Cy
Cuts at: 1014 1936 2077 1014
Size: 922 141 4389
Fragments arranged by size:
4389 922 141
PshAI GACnn'nnGTC
Cuts at: 1012 1012
Size: 5452
PstI C_TGCA'G
Cuts at: 350 479 970 1808 2497 3182 350
Size: 129 491 838 689 685 2620
Fragments arranged by size:
2620 838 689 685 491 129
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5452
PvuII CAG'CTG
Cuts at: 711 927 1101 1803 2092 2875 3235 711
Size: 216 174 702 289 783 360 2928
Fragments arranged by size:
2928 783 702 360 289 216 174
RsrII CG'GwC_CG
Cuts at: 3645 3645
Size: 5452
SacI G_AGCT'C
Cuts at: 839 2464 839
Size: 1625 3827
SacII CC_GC'GG
Cuts at: 1087 1087
Size: 5452
SalI G'TCGA_C
Cuts at: 2487 2487
Size: 5452
SanDI GG'GwC_CC
Cuts at: 1936 1936
Size: 5452
SapI GCTCTTCn'nnn_
Cuts at: 758 3473 3683 758
Size: 2715 210 2527
Fragments arranged by size:
2715 2527 210
SbfI CC_TGCA'GG
Cuts at: 2497 2497
Size: 5452
ScaI AGT'ACT
Cuts at: 4969 4969
Size: 5452
SexAI A'CCwGG_T
Cuts at: 2035 2035
Size: 5452
SfcI C'TryA_G
Cuts at: 33 346 475 966 1804 2493 3178 4173 4364
Size: 313 129 491 838 689 685 995 191
Cuts at: 4364 33
Size: 1121
Fragments arranged by size:
1121 995 838 689 685 491 313 191
129
SfiI GGCCn_nnn'nGGCC
Cuts at: 2357 2357
Size: 5452
SfoI GGC'GCC
Cuts at: 2882 3130 2882
Size: 248 5204
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5452
SmaI CCC'GGG
Cuts at: 1529 2472 1529
Size: 943 4509
SmlI C'TyrA_G
Cuts at: 667 2605 3520 4014 4276 667
Size: 1938 915 494 262 1843
Fragments arranged by size:
1938 1843 915 494 262
SnaBI TAC'GTA
Cuts at: 2442 2442
Size: 5452
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5452
SphI G_CATG'C
Cuts at: 2503 3003 2503
Size: 500 4952
StuI AGG'CCT
Cuts at: 694 2360 694
Size: 1666 3786
StyI C'CwwG_G
Cuts at: 305 996 1256 2355 2584 2938 305
Size: 691 260 1099 229 354 2819
Fragments arranged by size:
2819 1099 691 354 260 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1024 3033 3166 3625 3836 1024
Size: 2009 133 459 211 2640
Fragments arranged by size:
2640 2009 459 211 133
TatI w'GTAC_w
Cuts at: 1382 1577 1827 4967 1382
Size: 195 250 3140 1867
Fragments arranged by size:
3140 1867 250 195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 198 230 1937 1969 4969 5001 198
Size: 32 1707 32 3000 32 649
Fragments arranged by size:
3000 1707 649 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3247 3247
Size: 5452
XbaI T'CTAG_A
Cuts at: 2481 2481
Size: 5452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2293 2945 2293
Size: 652 4800
XmaI C'CCGG_G
Cuts at: 1527 2470 1527
Size: 943 4509
XmnI GAAnn'nnTTC
Cuts at: 445 773 445
Size: 328 5124
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmtI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI HindIII MfeI NdeI NheI NotI NsiI PacI
PasI PmlI PsiI PspOMI PspXI PsrI SgrAI SrfI
SspI SwaI XhoI ZraI