Restriction map for
pF1KSDA0529
(Circular) (Six-base) MAPSORT of: pF1KSDA0529.seq Check: 3149 from: 1 to: 5995
pF1KSDA0529 5995 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 3031 3031
Size: 5995
Acc65I G'GTAC_C
Cuts at: 215 3009 215
Size: 2794 3201
AclI AA'CG_TT
Cuts at: 5835 5835
Size: 5995
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 615 1865 1931 1940 2099 2468 2807 2876
Size: 187 1250 66 9 159 369 339 69
Cuts at: 2876 3818 4250 4999 5267 428
Size: 942 432 749 268 1156
Fragments arranged by size:
1250 1156 942 749 432 369 339 268
187 159 69 66 9
AflII C'TTAA_G
Cuts at: 361 361
Size: 5995
AflIII A'CryG_T
Cuts at: 928 3345 4451 5428 928
Size: 2417 1106 977 1495
Fragments arranged by size:
2417 1495 1106 977
AgeI A'CCGG_T
Cuts at: 4383 4383
Size: 5995
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2670 2670
Size: 5995
AlwNI CAG_nnn'CTG
Cuts at: 3298 4867 3298
Size: 1569 4426
ApaLI G'TGCA_C
Cuts at: 4765 4765
Size: 5995
ApoI r'AATT_y
Cuts at: 1017 1759 2626 2997 5348 5604 1017
Size: 742 867 371 2351 256 1408
Fragments arranged by size:
2351 1408 867 742 371 256
AseI AT'TA_AT
Cuts at: 20 1955 2636 20
Size: 1935 681 3379
Fragments arranged by size:
3379 1935 681
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5995
AvaI C'yCGr_G
Cuts at: 1770 3013 1770
Size: 1243 4752
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2576 2609 2576
Size: 33 5962
BamHI G'GATC_C
Cuts at: 1488 1924 2298 3018 1488
Size: 436 374 720 4465
Fragments arranged by size:
4465 720 436 374
BanI G'GyrC_C
Cuts at: 215 3009 3423 3671 3706 215
Size: 2794 414 248 35 2504
Fragments arranged by size:
2794 2504 414 248 35
BanII G_rGCy'C
Cuts at: 3007 4037 3007
Size: 1030 4965
BbeI G_GCGC'C
Cuts at: 3427 3675 3427
Size: 248 5747
BbsI GAAGACnn'nnnn_
Cuts at: 823 1853 2795 823
Size: 1030 942 4023
Fragments arranged by size:
4023 1030 942
BbvCI CC'TCA_GC
Cuts at: 2339 2339
Size: 5995
BciVI GTATCCnnnnn_n'
Cuts at: 317 2124 2577 3891 4654 5995 317
Size: 1807 453 1314 763 1341 317
Fragments arranged by size:
1807 1341 1314 763 453 317
BclI T'GATC_A
Cuts at: 1073 3513 1073
Size: 2440 3555
BglI GCCn_nnn'nGGC
Cuts at: 905 1973 905
Size: 1068 4927
BglII A'GATC_T
Cuts at: 1548 2275 3508 5547 1548
Size: 727 1233 2039 1996
Fragments arranged by size:
2039 1996 1233 727
BlpI GC'TnA_GC
Cuts at: 3105 3105
Size: 5995
Bme1580I G_kGCm'C
Cuts at: 3618 3711 4769 3618
Size: 93 1058 4844
Fragments arranged by size:
4844 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 348 3205 3286 3621 5767 348
Size: 2857 81 335 2146 576
Fragments arranged by size:
2857 2146 576 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4360 4360
Size: 5995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2507 2507
Size: 5995
Bpu10I CC'TnA_GC
Cuts at: 2260 2339 2260
Size: 79 5916
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1893 2527 3169 4048 4542 4840 1893
Size: 634 642 879 494 298 3048
Fragments arranged by size:
3048 879 642 634 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 646 5699 646
Size: 5053 942
BsaAI yAC'GTr
Cuts at: 929 1731 2985 3346 929
Size: 802 1254 361 3578
Fragments arranged by size:
3578 1254 802 361
BsaBI GATnn'nnATC
Cuts at: 6 1001 2297 3507 5546 6
Size: 995 1296 1210 2039 455
Fragments arranged by size:
2039 1296 1210 995 455
BsaHI Gr'CG_yC
Cuts at: 3424 3672 4376 5872 3424
Size: 248 704 1496 3547
Fragments arranged by size:
3547 1496 704 248
BsaWI w'CCGG_w
Cuts at: 197 3182 3703 4383 4657 4804 5215 197
Size: 2985 521 680 274 147 411 977
Fragments arranged by size:
2985 977 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2108 2717 2108
Size: 609 5386
BseYI C'CCAG_C
Cuts at: 158 3418 4176 4755 5251 158
Size: 3260 758 579 496 902
Fragments arranged by size:
3260 902 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 3535 3581 4791 104
Size: 3431 46 1210 1308
Fragments arranged by size:
3431 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1730 3007 3785 3975 4769 1730
Size: 1277 778 190 794 2956
Fragments arranged by size:
2956 1277 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 1730 3007 3618 3711 3785 3975 4037 4769 1730
Size: 1277 611 93 74 190 62 732 2956
Fragments arranged by size:
2956 1277 732 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 197 5215 197
Size: 5018 977
BspHI T'CATG_A
Cuts at: 935 5171 935
Size: 4236 1759
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1593 2638 3043 3257 3559 3940 1593
Size: 1045 405 214 302 381 3648
Fragments arranged by size:
3648 1045 405 381 302 214
BsrBI CCG'CTC
Cuts at: 50 142 4285 4339 5825 5990 50
Size: 92 4143 54 1486 165 55
Fragments arranged by size:
4143 1486 165 92 55 54
BsrDI GCAATG_nn'
Cuts at: 1438 2940 3905 1438
Size: 1502 965 3528
Fragments arranged by size:
3528 1502 965
BsrFI r'CCGG_y
Cuts at: 3991 4172 4383 3991
Size: 181 211 5603
Fragments arranged by size:
5603 211 181
BsrGI T'GTAC_A
Cuts at: 1555 1555
Size: 5995
BssSI C'ACGA_G
Cuts at: 4264 4624 4264
Size: 360 5635
BstAPI GCAn_nnn'nTGC
Cuts at: 1567 3370 1567
Size: 1803 4192
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5995
BstXI CCAn_nnnn'nTGG
Cuts at: 1789 1789
Size: 5995
BstYI r'GATC_y
Cuts at: 1 147 1488 1548 1924 2275 2298 2451 3018
Size: 146 1341 60 376 351 23 153 567
Cuts at: 3018 3485 3508 3843 4089 5103 5189 5201 5547
Size: 467 23 335 246 1014 86 12 346
Cuts at: 5547 1
Size: 449
Fragments arranged by size:
1341 1014 567 467 449 376 351 346
335 246 153 146 86 60 23 23
12
Bsu36I CC'TnA_GG
Cuts at: 1032 1032
Size: 5995
BtgI C'CryG_G
Cuts at: 125 125
Size: 5995
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 156 3943 4124 4287 156
Size: 3787 181 163 1864
Fragments arranged by size:
3787 1864 181 163
BtsI GCAGTG_nn'
Cuts at: 501 1932 2777 3236 501
Size: 1431 845 459 3260
Fragments arranged by size:
3260 1431 845 459
DraI TTT'AAA
Cuts at: 1319 2992 1319
Size: 1673 4322
DraIII CAC_nnn'GTG
Cuts at: 1999 1999
Size: 5995
DrdI GACnn_nn'nnGTC
Cuts at: 2770 3699 4559 2770
Size: 929 860 4206
Fragments arranged by size:
4206 929 860
EaeI y'GGCC_r
Cuts at: 784 3578 3752 4143 4170 5517 784
Size: 2794 174 391 27 1347 1262
Fragments arranged by size:
2794 1347 1262 391 174 27
EagI C'GGCC_G
Cuts at: 3578 3578
Size: 5995
EarI CTCTTCn'nnn_
Cuts at: 2827 4016 4226 2827
Size: 1189 210 4596
Fragments arranged by size:
4596 1189 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 152 160 4201 4513 4659 5805 145
Size: 7 8 4041 312 146 1146 335
Fragments arranged by size:
4041 1146 335 312 146 8 7
EcoICRI GAG'CTC
Cuts at: 3005 3005
Size: 5995
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 615 1865 1931 1940 2099 2468 2507 2807
Size: 187 1250 66 9 159 369 39 300
Cuts at: 2807 2876 3818 4250 4999 5267 428
Size: 69 942 432 749 268 1156
Fragments arranged by size:
1250 1156 942 749 432 369 300 268
187 159 69 66 39 9
EcoO109I rG'GnC_Cy
Cuts at: 793 2650 3132 793
Size: 1857 482 3656
Fragments arranged by size:
3656 1857 482
EcoRI G'AATT_C
Cuts at: 2626 2997 2626
Size: 371 5624
EcoRV GAT'ATC
Cuts at: 1165 2208 1165
Size: 1043 4952
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 812 1870 4012 812
Size: 1058 2142 2795
Fragments arranged by size:
2795 2142 1058
FspI TGC'GCA
Cuts at: 3774 3774
Size: 5995
HaeII r_GCGC'y
Cuts at: 3427 3675 4699 5678 3427
Size: 248 1024 979 3744
Fragments arranged by size:
3744 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 279 311 340 372 435 467 2014 2046 2260
Size: 32 29 32 63 32 1547 32 214
Cuts at: 2260 2292 2497 2529 3835 3865 3867 3897 279
Size: 32 205 32 1306 30 2 30 2377
Fragments arranged by size:
2377 1547 1306 214 205 63 32 32
32 32 32 32 30 30 29 2
HincII GTy'rAC
Cuts at: 2146 3032 2146
Size: 886 5109
KasI G'GCGC_C
Cuts at: 3423 3671 3423
Size: 248 5747
KpnI G_GTAC'C
Cuts at: 219 3013 219
Size: 2794 3201
MfeI C'AATT_G
Cuts at: 605 605
Size: 5995
MluI A'CGCG_T
Cuts at: 5428 5428
Size: 5995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 394 2070 2561 2603 2705 4666 4850 394
Size: 1676 491 42 102 1961 184 1539
Fragments arranged by size:
1961 1676 1539 491 184 102 42
MscI TGG'CCA
Cuts at: 786 3754 786
Size: 2968 3027
MslI CAynn'nnrTG
Cuts at: 442 2360 3982 4109 442
Size: 1918 1622 127 2328
Fragments arranged by size:
2328 1918 1622 127
MspA1I CmG'CkG
Cuts at: 162 431 2896 3104 3418 3778 4793 5038 162
Size: 269 2465 208 314 360 1015 245 1119
Fragments arranged by size:
2465 1119 1015 360 314 269 245 208
NaeI GCC'GGC
Cuts at: 4174 4174
Size: 5995
NarI GG'CG_CC
Cuts at: 3424 3672 3424
Size: 248 5747
NcoI C'CATG_G
Cuts at: 125 125
Size: 5995
NdeI CA'TA_TG
Cuts at: 2814 2814
Size: 5995
NgoMIV G'CCGG_C
Cuts at: 4172 4172
Size: 5995
NruI TCG'CGA
Cuts at: 175 4356 175
Size: 4181 1814
NspI r_CATG'y
Cuts at: 3046 3546 4455 3046
Size: 500 909 4586
Fragments arranged by size:
4586 909 500
PacI TTA_AT'TAA
Cuts at: 2636 2636
Size: 5995
PciI A'CATG_T
Cuts at: 4451 4451
Size: 5995
PflMI CCAn_nnn'nTGG
Cuts at: 662 662
Size: 5995
PfoI T'CCnGG_A
Cuts at: 309 309
Size: 5995
PmeI GTTT'AAAC
Cuts at: 2992 2992
Size: 5995
PmlI CAC'GTG
Cuts at: 1731 1731
Size: 5995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5134 5166 5134
Size: 32 5963
PpuMI rG'GwC_Cy
Cuts at: 793 2650 793
Size: 1857 4138
PsiI TTA'TAA
Cuts at: 851 1859 851
Size: 1008 4987
PspXI vC'TCGA_Gb
Cuts at: 1770 1770
Size: 5995
PstI C_TGCA'G
Cuts at: 1590 3040 3725 1590
Size: 1450 685 3860
Fragments arranged by size:
3860 1450 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5995
PvuII CAG'CTG
Cuts at: 431 2896 3418 3778 431
Size: 2465 522 360 2648
Fragments arranged by size:
2648 2465 522 360
RsrII CG'GwC_CG
Cuts at: 4188 4188
Size: 5995
SacI G_AGCT'C
Cuts at: 3007 3007
Size: 5995
SalI G'TCGA_C
Cuts at: 3030 3030
Size: 5995
SapI GCTCTTCn'nnn_
Cuts at: 4016 4226 4016
Size: 210 5785
SbfI CC_TGCA'GG
Cuts at: 3040 3040
Size: 5995
ScaI AGT'ACT
Cuts at: 2528 5512 2528
Size: 2984 3011
SexAI A'CCwGG_T
Cuts at: 211 211
Size: 5995
SfcI C'TryA_G
Cuts at: 33 1586 3036 3721 4716 4907 33
Size: 1553 1450 685 995 191 1121
Fragments arranged by size:
1553 1450 1121 995 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 905 905
Size: 5995
SfoI GGC'GCC
Cuts at: 3425 3673 3425
Size: 248 5747
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5995
SmaI CCC'GGG
Cuts at: 3015 3015
Size: 5995
SmlI C'TyrA_G
Cuts at: 361 1770 1872 2542 3148 4063 4557 4819 361
Size: 1409 102 670 606 915 494 262 1537
Fragments arranged by size:
1537 1409 915 670 606 494 262 102
SnaBI TAC'GTA
Cuts at: 2985 2985
Size: 5995
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5995
SphI G_CATG'C
Cuts at: 3046 3546 3046
Size: 500 5495
SspI AAT'ATT
Cuts at: 1637 1953 1637
Size: 316 5679
StuI AGG'CCT
Cuts at: 899 899
Size: 5995
StyI C'CwwG_G
Cuts at: 125 738 787 1463 3127 3481 125
Size: 613 49 676 1664 354 2639
Fragments arranged by size:
2639 1664 676 613 354 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 172 2888 3576 3709 4168 4379 172
Size: 2716 688 133 459 211 1788
Fragments arranged by size:
2716 1788 688 459 211 133
TatI w'GTAC_w
Cuts at: 1511 1555 2122 2526 2535 2817 5510 1511
Size: 44 567 404 9 282 2693 1996
Fragments arranged by size:
2693 1996 567 404 282 44 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5512 5544 5512
Size: 32 5963
Tth111I GACn'n_nGTC
Cuts at: 3790 3790
Size: 5995
XbaI T'CTAG_A
Cuts at: 3024 3024
Size: 5995
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 410 1954 2499 3488 410
Size: 1544 545 989 2917
Fragments arranged by size:
2917 1544 989 545
XhoI C'TCGA_G
Cuts at: 1770 1770
Size: 5995
XmaI C'CCGG_G
Cuts at: 3013 3013
Size: 5995
XmnI GAAnn'nnTTC
Cuts at: 1329 1329
Size: 5995
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NruI NspI PacI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsiI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BcgI BfrBI BmgBI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BssHII BstEII BstZ17I ClaI CspCI EcoNI
FseI FspAI HindIII HpaI NheI NotI NsiI PasI
PshAI PspOMI PsrI SacII SanDI SgrAI SrfI SwaI
ZraI