Restriction map for
pF1KSDA0535
(Circular) (Six-base) MAPSORT of: pF1KSDA0535.seq Check: 1473 from: 1 to: 6280
pF1KSDA0535 6280 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 3316 3316
Size: 6280
Acc65I G'GTAC_C
Cuts at: 3294 3294
Size: 6280
AclI AA'CG_TT
Cuts at: 6120 6120
Size: 6280
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 272 860 2864 2959 4103 4535 5284 5552
Size: 111 588 2004 95 1144 432 749 268
Cuts at: 5552 161
Size: 889
Fragments arranged by size:
2004 1144 889 749 588 432 268 111
95
AflII C'TTAA_G
Cuts at: 1971 2422 1971
Size: 451 5829
AflIII A'CryG_T
Cuts at: 1257 3630 4736 5713 1257
Size: 2373 1106 977 1824
Fragments arranged by size:
2373 1824 1106 977
AgeI A'CCGG_T
Cuts at: 4668 4668
Size: 6280
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 639 1070 639
Size: 431 5849
AlwNI CAG_nnn'CTG
Cuts at: 139 1849 1852 1920 2638 3583 5152 139
Size: 1710 3 68 718 945 1569 1267
Fragments arranged by size:
1710 1569 1267 945 718 68 3
ApaLI G'TGCA_C
Cuts at: 5050 5050
Size: 6280
ApoI r'AATT_y
Cuts at: 529 567 850 1029 1328 1549 1744 2202 3147
Size: 38 283 179 299 221 195 458 945
Cuts at: 3147 3282 5633 5889 529
Size: 135 2351 256 920
Fragments arranged by size:
2351 945 920 458 299 283 256 221
195 179 135 38
AseI AT'TA_AT
Cuts at: 20 20
Size: 6280
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6280
AvaI C'yCGr_G
Cuts at: 2451 3298 2451
Size: 847 5433
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 471 504 471
Size: 33 6247
BamHI G'GATC_C
Cuts at: 3303 3303
Size: 6280
BanI G'GyrC_C
Cuts at: 180 1298 1771 3294 3708 3956 3991 180
Size: 1118 473 1523 414 248 35 2469
Fragments arranged by size:
2469 1523 1118 473 414 248 35
BanII G_rGCy'C
Cuts at: 209 500 1271 1789 2194 3292 4322 209
Size: 291 771 518 405 1098 1030 2167
Fragments arranged by size:
2167 1098 1030 771 518 405 291
BbeI G_GCGC'C
Cuts at: 1775 3712 3960 1775
Size: 1937 248 4095
Fragments arranged by size:
4095 1937 248
BbsI GAAGACnn'nnnn_
Cuts at: 347 427 479 1179 1835 2164 2561 347
Size: 80 52 700 656 329 397 4066
Fragments arranged by size:
4066 700 656 397 329 80 52
BciVI GTATCCnnnnn_n'
Cuts at: 3268 4176 4939 6280 3268
Size: 908 763 1341 3268
Fragments arranged by size:
3268 1341 908 763
BclI T'GATC_A
Cuts at: 3798 3798
Size: 6280
BfrBI ATG'CAT
Cuts at: 1341 2344 1341
Size: 1003 5277
BglII A'GATC_T
Cuts at: 2271 3793 5832 2271
Size: 1522 2039 2719
Fragments arranged by size:
2719 2039 1522
BlpI GC'TnA_GC
Cuts at: 1067 3390 1067
Size: 2323 3957
Bme1580I G_kGCm'C
Cuts at: 1231 1303 2459 3903 3996 5054 1231
Size: 72 1156 1444 93 1058 2457
Fragments arranged by size:
2457 1444 1156 1058 93 72
BmrI ACTGGGnnnn_n'
Cuts at: 1508 2179 2885 3490 3571 3906 6052 1508
Size: 671 706 605 81 335 2146 1736
Fragments arranged by size:
2146 1736 706 671 605 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2710 4645 2710
Size: 1935 4345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 444 1063 1141 2246 444
Size: 619 78 1105 4478
Fragments arranged by size:
4478 1105 619 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1339 3454 4333 4827 5125 1339
Size: 2115 879 494 298 2494
Fragments arranged by size:
2494 2115 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1213 5984 1213
Size: 4771 1509
BsaAI yAC'GTr
Cuts at: 1260 2328 2460 3270 3631 1260
Size: 1068 132 810 361 3909
Fragments arranged by size:
3909 1068 810 361 132
BsaBI GATnn'nnATC
Cuts at: 6 1475 3792 5831 6
Size: 1469 2317 2039 455
Fragments arranged by size:
2317 2039 1469 455
BsaHI Gr'CG_yC
Cuts at: 1772 3709 3957 4661 6157 1772
Size: 1937 248 704 1496 1895
Fragments arranged by size:
1937 1895 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2494 3467 3988 4668 4942 5089 5500 2494
Size: 973 521 680 274 147 411 3274
Fragments arranged by size:
3274 973 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 727 757 1210 1240 1885 1915 2410 2440 727
Size: 30 453 30 645 30 495 30 4567
Fragments arranged by size:
4567 645 495 453 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 678 976 2179 2182 3034 3037 678
Size: 298 1203 3 852 3 3921
Fragments arranged by size:
3921 1203 852 298 3 3
BseYI C'CCAG_C
Cuts at: 731 938 2120 2754 3703 4461 5040 5536 731
Size: 207 1182 634 949 758 579 496 1475
Fragments arranged by size:
1475 1182 949 758 634 579 496 207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 740 1787 1991 431
Size: 309 1047 204 4720
Fragments arranged by size:
4720 1047 309 204
BsiEI CG_ry'CG
Cuts at: 104 3820 3866 5076 104
Size: 3716 46 1210 1308
Fragments arranged by size:
3716 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 209 500 1127 1355 2194 3292 4070 4260 5054
Size: 291 627 228 839 1098 778 190 794
Cuts at: 5054 209
Size: 1435
Fragments arranged by size:
1435 1098 839 794 778 627 291 228
190
BsmI GAATG_Cn'
Cuts at: 912 2072 2089 3220 912
Size: 1160 17 1131 3972
Fragments arranged by size:
3972 1160 1131 17
Bsp1286I G_dGCh'C
Cuts at: 209 500 1127 1231 1271 1303 1355 1789 2194
Size: 291 627 104 40 32 52 434 405
Cuts at: 2194 2459 3292 3903 3996 4070 4260 4322 5054
Size: 265 833 611 93 74 190 62 732
Cuts at: 5054 209
Size: 1435
Fragments arranged by size:
1435 833 732 627 611 434 405 291
265 190 104 93 74 62 52 40
32
BspEI T'CCGG_A
Cuts at: 2494 5500 2494
Size: 3006 3274
BspHI T'CATG_A
Cuts at: 410 2852 5456 410
Size: 2442 2604 1234
Fragments arranged by size:
2604 2442 1234
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3064 3328 3542 3844 4225 3064
Size: 264 214 302 381 5119
Fragments arranged by size:
5119 381 302 264 214
BsrBI CCG'CTC
Cuts at: 50 4570 4624 6110 6275 50
Size: 4520 54 1486 165 55
Fragments arranged by size:
4520 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 939 1466 2754 4190 939
Size: 527 1288 1436 3029
Fragments arranged by size:
3029 1436 1288 527
BsrFI r'CCGG_y
Cuts at: 4276 4457 4668 4276
Size: 181 211 5888
Fragments arranged by size:
5888 211 181
BsrGI T'GTAC_A
Cuts at: 3182 3182
Size: 6280
BssSI C'ACGA_G
Cuts at: 2504 4549 4909 2504
Size: 2045 360 3875
Fragments arranged by size:
3875 2045 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1849 1852 3655 1849
Size: 3 1803 4474
Fragments arranged by size:
4474 1803 3
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6280
BstXI CCAn_nnnn'nTGG
Cuts at: 1789 1789
Size: 6280
BstYI r'GATC_y
Cuts at: 1 2271 3303 3770 3793 4128 4374 5388 5474
Size: 2270 1032 467 23 335 246 1014 86
Cuts at: 5474 5486 5832 1
Size: 12 346 449
Fragments arranged by size:
2270 1032 1014 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 230 125
Size: 105 6175
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 623 2335 2459 4228 4409 4572 623
Size: 1712 124 1769 181 163 2331
Fragments arranged by size:
2331 1769 1712 181 163 124
BtsI GCAGTG_nn'
Cuts at: 354 1510 2361 3254 3521 354
Size: 1156 851 893 267 3113
Fragments arranged by size:
3113 1156 893 851 267
DraI TTT'AAA
Cuts at: 572 596 3277 572
Size: 24 2681 3575
Fragments arranged by size:
3575 2681 24
DraIII CAC_nnn'GTG
Cuts at: 1311 1311
Size: 6280
DrdI GACnn_nn'nnGTC
Cuts at: 3984 4844 3984
Size: 860 5420
EaeI y'GGCC_r
Cuts at: 1791 2322 2592 3863 4037 4428 4455 5802 1791
Size: 531 270 1271 174 391 27 1347 2269
Fragments arranged by size:
2269 1347 1271 531 391 270 174 27
EagI C'GGCC_G
Cuts at: 3863 3863
Size: 6280
EarI CTCTTCn'nnn_
Cuts at: 235 645 992 2055 4301 4511 235
Size: 410 347 1063 2246 210 2004
Fragments arranged by size:
2246 2004 1063 410 347 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4486 4798 4944 6090 4486
Size: 312 146 1146 4676
Fragments arranged by size:
4676 1146 312 146
EcoICRI GAG'CTC
Cuts at: 207 498 2192 3290 207
Size: 291 1694 1098 3197
Fragments arranged by size:
3197 1694 1098 291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 272 444 860 1063 1141 2246 2864 2959
Size: 111 172 416 203 78 1105 618 95
Cuts at: 2959 4103 4535 5284 5552 161
Size: 1144 432 749 268 889
Fragments arranged by size:
1144 1105 889 749 618 432 416 268
203 172 111 95 78
EcoNI CCTnn'n_nnAGG
Cuts at: 1015 1766 2579 1015
Size: 751 813 4716
Fragments arranged by size:
4716 813 751
EcoO109I rG'GnC_Cy
Cuts at: 3130 3417 3130
Size: 287 5993
EcoRI G'AATT_C
Cuts at: 1549 3282 1549
Size: 1733 4547
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4297 4297
Size: 6280
FspI TGC'GCA
Cuts at: 4059 4059
Size: 6280
HaeII r_GCGC'y
Cuts at: 1775 3712 3960 4984 5963 1775
Size: 1937 248 1024 979 2092
Fragments arranged by size:
2092 1937 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 466 498 727 759 1885 1917 2792 2824 4120
Size: 32 229 32 1126 32 875 32 1296
Cuts at: 4120 4150 4152 4182 466
Size: 30 2 30 2564
Fragments arranged by size:
2564 1296 1126 875 229 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 279 3317 279
Size: 3038 3242
HindIII A'AGCT_T
Cuts at: 2257 2487 2790 2257
Size: 230 303 5747
Fragments arranged by size:
5747 303 230
KasI G'GCGC_C
Cuts at: 1771 3708 3956 1771
Size: 1937 248 4095
Fragments arranged by size:
4095 1937 248
KpnI G_GTAC'C
Cuts at: 3298 3298
Size: 6280
MfeI C'AATT_G
Cuts at: 1052 1436 1052
Size: 384 5896
MluI A'CGCG_T
Cuts at: 5713 5713
Size: 6280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 770 1928 2062 2323 2455 2966 4951 5135 770
Size: 1158 134 261 132 511 1985 184 1915
Fragments arranged by size:
1985 1915 1158 511 261 184 134 132
MscI TGG'CCA
Cuts at: 2324 2594 4039 2324
Size: 270 1445 4565
Fragments arranged by size:
4565 1445 270
MslI CAynn'nnrTG
Cuts at: 641 1346 2307 2354 2912 2992 3178 4267 4394
Size: 705 961 47 558 80 186 1089 127
Cuts at: 4394 641
Size: 2527
Fragments arranged by size:
2527 1089 961 705 558 186 127 80
47
MspA1I CmG'CkG
Cuts at: 250 1447 1849 1877 3389 3703 4063 5078 5323
Size: 1197 402 28 1512 314 360 1015 245
Cuts at: 5323 250
Size: 1207
Fragments arranged by size:
1512 1207 1197 1015 402 360 314 245
28
NaeI GCC'GGC
Cuts at: 4459 4459
Size: 6280
NarI GG'CG_CC
Cuts at: 1772 3709 3957 1772
Size: 1937 248 4095
Fragments arranged by size:
4095 1937 248
NcoI C'CATG_G
Cuts at: 125 230 125
Size: 105 6175
NdeI CA'TA_TG
Cuts at: 3159 3159
Size: 6280
NgoMIV G'CCGG_C
Cuts at: 4457 4457
Size: 6280
NruI TCG'CGA
Cuts at: 4641 4641
Size: 6280
NsiI A_TGCA'T
Cuts at: 1343 2346 1343
Size: 1003 5277
NspI r_CATG'y
Cuts at: 1927 3331 3831 4740 1927
Size: 1404 500 909 3467
Fragments arranged by size:
3467 1404 909 500
PciI A'CATG_T
Cuts at: 4736 4736
Size: 6280
PfoI T'CCnGG_A
Cuts at: 200 1994 2223 2698 200
Size: 1794 229 475 3782
Fragments arranged by size:
3782 1794 475 229
PmeI GTTT'AAAC
Cuts at: 3277 3277
Size: 6280
PmlI CAC'GTG
Cuts at: 1260 2328 2460 1260
Size: 1068 132 5080
Fragments arranged by size:
5080 1068 132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5419 5451 5419
Size: 32 6248
PpuMI rG'GwC_Cy
Cuts at: 3130 3130
Size: 6280
PstI C_TGCA'G
Cuts at: 1064 1446 1882 3325 4010 1064
Size: 382 436 1443 685 3334
Fragments arranged by size:
3334 1443 685 436 382
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6280
PvuII CAG'CTG
Cuts at: 250 1447 1849 3703 4063 250
Size: 1197 402 1854 360 2467
Fragments arranged by size:
2467 1854 1197 402 360
RsrII CG'GwC_CG
Cuts at: 4473 4473
Size: 6280
SacI G_AGCT'C
Cuts at: 209 500 2194 3292 209
Size: 291 1694 1098 3197
Fragments arranged by size:
3197 1694 1098 291
SalI G'TCGA_C
Cuts at: 3315 3315
Size: 6280
SapI GCTCTTCn'nnn_
Cuts at: 4301 4511 4301
Size: 210 6070
SbfI CC_TGCA'GG
Cuts at: 3325 3325
Size: 6280
ScaI AGT'ACT
Cuts at: 5797 5797
Size: 6280
SfcI C'TryA_G
Cuts at: 33 315 1060 1442 1878 2139 3321 4006 5001
Size: 282 745 382 436 261 1182 685 995
Cuts at: 5001 5192 33
Size: 191 1121
Fragments arranged by size:
1182 1121 995 745 685 436 382 282
261 191
SfoI GGC'GCC
Cuts at: 1773 3710 3958 1773
Size: 1937 248 4095
Fragments arranged by size:
4095 1937 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6280
SmaI CCC'GGG
Cuts at: 3300 3300
Size: 6280
SmlI C'TyrA_G
Cuts at: 1354 1971 2422 3433 4348 4842 5104 1354
Size: 617 451 1011 915 494 262 2530
Fragments arranged by size:
2530 1011 915 617 494 451 262
SnaBI TAC'GTA
Cuts at: 3270 3270
Size: 6280
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6280
SphI G_CATG'C
Cuts at: 1927 3331 3831 1927
Size: 1404 500 4376
Fragments arranged by size:
4376 1404 500
StuI AGG'CCT
Cuts at: 1206 2233 1206
Size: 1027 5253
StyI C'CwwG_G
Cuts at: 125 230 374 1022 1201 1928 2540 2595 3412
Size: 105 144 648 179 727 612 55 817
Cuts at: 3412 3766 125
Size: 354 2639
Fragments arranged by size:
2639 817 727 648 612 354 179 144
105 55
SwaI ATTT'AAAT
Cuts at: 572 572
Size: 6280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1156 1426 1795 1849 2797 3861 3994 4453 4664
Size: 270 369 54 948 1064 133 459 211
Cuts at: 4664 1156
Size: 2772
Fragments arranged by size:
2772 1064 948 459 369 270 211 133
54
TatI w'GTAC_w
Cuts at: 3182 5795 3182
Size: 2613 3667
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 164 196 761 793 1210 1242 2019 2051 2410
Size: 32 565 32 417 32 777 32 359
Cuts at: 2410 2442 2505 2537 5797 5829 164
Size: 32 63 32 3260 32 615
Fragments arranged by size:
3260 777 615 565 417 359 63 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4075 4075
Size: 6280
XbaI T'CTAG_A
Cuts at: 2196 3309 2196
Size: 1113 5167
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3773 3773
Size: 6280
XmaI C'CCGG_G
Cuts at: 3298 3298
Size: 6280
XmnI GAAnn'nnTTC
Cuts at: 434 434
Size: 6280
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NruI NsiI NspI PciI PfoI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BbvCI BcgI BglI BmgBI BmtI Bpu10I BsiWI
BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HpaI NheI NotI PacI PasI PflMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI XhoI ZraI