Restriction map for
pF1KSDA0537
(Circular) (Six-base) MAPSORT of: pF1KSDA0537.seq Check: 9587 from: 1 to: 5122
pF1KSDA0537 5122 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 2072 2158 2072
Size: 86 5036
Acc65I G'GTAC_C
Cuts at: 2006 2136 2006
Size: 130 4992
AclI AA'CG_TT
Cuts at: 757 4962 757
Size: 4205 917
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1249 1348 2020 2945 3377 4126 4394 1249
Size: 99 672 925 432 749 268 1977
Fragments arranged by size:
1977 925 749 672 432 268 99
AfeI AGC'GCT
Cuts at: 2084 2084
Size: 5122
AflIII A'CryG_T
Cuts at: 2472 3578 4555 2472
Size: 1106 977 3039
Fragments arranged by size:
3039 1106 977
AgeI A'CCGG_T
Cuts at: 3510 3510
Size: 5122
AhdI GACnn_n'nnGTC
Cuts at: 1095 1095
Size: 5122
AlwNI CAG_nnn'CTG
Cuts at: 1282 1786 1998 2425 3994 1282
Size: 504 212 427 1569 2410
Fragments arranged by size:
2410 1569 504 427 212
ApaI G_GGCC'C
Cuts at: 840 840
Size: 5122
ApaLI G'TGCA_C
Cuts at: 622 3892 622
Size: 3270 1852
ApoI r'AATT_y
Cuts at: 2124 4475 4731 2124
Size: 2351 256 2515
Fragments arranged by size:
2515 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5122
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5122
AvaI C'yCGr_G
Cuts at: 194 962 2140 194
Size: 768 1178 3176
Fragments arranged by size:
3176 1178 768
BamHI G'GATC_C
Cuts at: 1322 2145 1322
Size: 823 4299
BanI G'GyrC_C
Cuts at: 180 316 1127 1473 1726 2006 2136 2550 2798
Size: 136 811 346 253 280 130 414 248
Cuts at: 2798 2833 180
Size: 35 2469
Fragments arranged by size:
2469 811 414 346 280 253 248 136
130 35
BanII G_rGCy'C
Cuts at: 190 233 840 1973 2134 3164 190
Size: 43 607 1133 161 1030 2148
Fragments arranged by size:
2148 1133 1030 607 161 43
BbeI G_GCGC'C
Cuts at: 184 2554 2802 184
Size: 2370 248 2504
Fragments arranged by size:
2504 2370 248
BbsI GAAGACnn'nnnn_
Cuts at: 1577 1577
Size: 5122
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1879 1913 1879
Size: 34 5088
BciVI GTATCCnnnnn_n'
Cuts at: 3018 3781 5122 3018
Size: 763 1341 3018
Fragments arranged by size:
3018 1341 763
BclI T'GATC_A
Cuts at: 512 2640 512
Size: 2128 2994
BfrBI ATG'CAT
Cuts at: 781 781
Size: 5122
BglII A'GATC_T
Cuts at: 2090 2635 4674 2090
Size: 545 2039 2538
Fragments arranged by size:
2538 2039 545
BlpI GC'TnA_GC
Cuts at: 2232 2232
Size: 5122
Bme1580I G_kGCm'C
Cuts at: 626 840 1729 2745 2838 3896 626
Size: 214 889 1016 93 1058 1852
Fragments arranged by size:
1852 1058 1016 889 214 93
BmrI ACTGGGnnnn_n'
Cuts at: 1056 1986 2332 2413 2748 4894 1056
Size: 930 346 81 335 2146 1284
Fragments arranged by size:
2146 1284 930 346 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1371 1824 3487 1371
Size: 453 1663 3006
Fragments arranged by size:
3006 1663 453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1933 1957 2107 247
Size: 1686 24 150 3262
Fragments arranged by size:
3262 1686 150 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2296 3175 3669 3967 2296
Size: 879 494 298 3451
Fragments arranged by size:
3451 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 295 580 798 4826 295
Size: 285 218 4028 591
Fragments arranged by size:
4028 591 285 218
BsaAI yAC'GTr
Cuts at: 2112 2473 2112
Size: 361 4761
BsaBI GATnn'nnATC
Cuts at: 6 439 511 2634 4673 6
Size: 433 72 2123 2039 455
Fragments arranged by size:
2123 2039 455 433 72
BsaHI Gr'CG_yC
Cuts at: 181 570 2551 2799 3503 4999 181
Size: 389 1981 248 704 1496 304
Fragments arranged by size:
1981 1496 704 389 304 248
BsaWI w'CCGG_w
Cuts at: 2009 2309 2830 3510 3784 3931 4342 2009
Size: 300 521 680 274 147 411 2789
Fragments arranged by size:
2789 680 521 411 300 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1660 1690 1660
Size: 30 5092
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1438 2032 1438
Size: 594 4528
BseYI C'CCAG_C
Cuts at: 174 833 1538 1636 1650 1737 2545 3303 3882
Size: 659 705 98 14 87 808 758 579
Cuts at: 3882 4378 174
Size: 496 918
Fragments arranged by size:
918 808 758 705 659 579 496 98
87 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1452 1452
Size: 5122
BsiEI CG_ry'CG
Cuts at: 104 1002 1225 2662 2708 3918 104
Size: 898 223 1437 46 1210 1308
Fragments arranged by size:
1437 1308 1210 898 223 46
BsiHKAI G_wGCw'C
Cuts at: 626 1448 2134 2912 3102 3896 626
Size: 822 686 778 190 794 1852
Fragments arranged by size:
1852 822 794 778 686 190
BsmBI CGTCTCn'nnnn_
Cuts at: 618 1935 618
Size: 1317 3805
Bsp1286I G_dGCh'C
Cuts at: 190 233 626 840 1448 1729 1973 2134 2745
Size: 43 393 214 608 281 244 161 611
Cuts at: 2745 2838 2912 3102 3164 3896 190
Size: 93 74 190 62 732 1416
Fragments arranged by size:
1416 732 611 608 393 281 244 214
190 161 93 74 62 43
BspEI T'CCGG_A
Cuts at: 4342 4342
Size: 5122
BspHI T'CATG_A
Cuts at: 4298 4298
Size: 5122
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2170 2384 2686 3067 2170
Size: 214 302 381 4225
Fragments arranged by size:
4225 381 302 214
BsrBI CCG'CTC
Cuts at: 50 564 1215 3412 3466 4952 5117 50
Size: 514 651 2197 54 1486 165 55
Fragments arranged by size:
2197 1486 651 514 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1022 3032 1022
Size: 2010 3112
BsrFI r'CCGG_y
Cuts at: 1979 2015 3118 3299 3510 1979
Size: 36 1103 181 211 3591
Fragments arranged by size:
3591 1103 211 181 36
BssSI C'ACGA_G
Cuts at: 3391 3751 3391
Size: 360 4762
BstAPI GCAn_nnn'nTGC
Cuts at: 2497 2497
Size: 5122
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5122
BstEII G'GTnAC_C
Cuts at: 1659 1659
Size: 5122
BstYI r'GATC_y
Cuts at: 1 1322 1952 2090 2145 2612 2635 2970 3216
Size: 1321 630 138 55 467 23 335 246
Cuts at: 3216 4230 4316 4328 4674 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1321 1014 630 467 449 346 335 246
138 86 55 23 12
BtgI C'CryG_G
Cuts at: 125 1730 125
Size: 1605 3517
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1336 1859 3070 3251 3414 1336
Size: 523 1211 181 163 3044
Fragments arranged by size:
3044 1211 523 181 163
BtsI GCAGTG_nn'
Cuts at: 1283 1584 2363 1283
Size: 301 779 4042
Fragments arranged by size:
4042 779 301
DraI TTT'AAA
Cuts at: 2119 2119
Size: 5122
DraIII CAC_nnn'GTG
Cuts at: 249 249
Size: 5122
DrdI GACnn_nn'nnGTC
Cuts at: 2826 3686 2826
Size: 860 4262
EaeI y'GGCC_r
Cuts at: 357 1181 1502 2705 2879 3270 3297 4644 357
Size: 824 321 1203 174 391 27 1347 835
Fragments arranged by size:
1347 1203 835 824 391 321 174 27
EagI C'GGCC_G
Cuts at: 2705 2705
Size: 5122
EarI CTCTTCn'nnn_
Cuts at: 1569 3143 3353 1569
Size: 1574 210 3338
Fragments arranged by size:
3338 1574 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3328 3640 3786 4932 3328
Size: 312 146 1146 3518
Fragments arranged by size:
3518 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2132 2132
Size: 5122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1249 1348 1933 1957 2020 2107 2945 3377
Size: 1002 99 585 24 63 87 838 432
Cuts at: 3377 4126 4394 247
Size: 749 268 975
Fragments arranged by size:
1002 975 838 749 585 432 268 99
87 63 24
EcoNI CCTnn'n_nnAGG
Cuts at: 812 1683 812
Size: 871 4251
EcoO109I rG'GnC_Cy
Cuts at: 837 1176 1803 2259 837
Size: 339 627 456 3700
Fragments arranged by size:
3700 627 456 339
EcoRI G'AATT_C
Cuts at: 2124 2124
Size: 5122
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 291 1713 3139 291
Size: 1422 1426 2274
Fragments arranged by size:
2274 1426 1422
FspI TGC'GCA
Cuts at: 2901 2901
Size: 5122
HaeII r_GCGC'y
Cuts at: 184 2086 2554 2802 3826 4805 184
Size: 1902 468 248 1024 979 501
Fragments arranged by size:
1902 1024 979 501 468 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 430 462 1081 1113 2962 2992 2994 3024 430
Size: 32 619 32 1849 30 2 30 2528
Fragments arranged by size:
2528 1849 619 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2159 2159
Size: 5122
KasI G'GCGC_C
Cuts at: 180 2550 2798 180
Size: 2370 248 2504
Fragments arranged by size:
2504 2370 248
KpnI G_GTAC'C
Cuts at: 2010 2140 2010
Size: 130 4992
MluI A'CGCG_T
Cuts at: 4555 4555
Size: 5122
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 749 1577 3793 3977 749
Size: 828 2216 184 1894
Fragments arranged by size:
2216 1894 828 184
MscI TGG'CCA
Cuts at: 1183 2881 1183
Size: 1698 3424
MslI CAynn'nnrTG
Cuts at: 2050 3109 3236 2050
Size: 1059 127 3936
Fragments arranged by size:
3936 1059 127
MspA1I CmG'CkG
Cuts at: 833 926 1221 1723 1925 2231 2545 2905 3920
Size: 93 295 502 202 306 314 360 1015
Cuts at: 3920 4165 833
Size: 245 1790
Fragments arranged by size:
1790 1015 502 360 314 306 295 245
202 93
NaeI GCC'GGC
Cuts at: 3301 3301
Size: 5122
NarI GG'CG_CC
Cuts at: 181 2551 2799 181
Size: 2370 248 2504
Fragments arranged by size:
2504 2370 248
NcoI C'CATG_G
Cuts at: 125 1730 125
Size: 1605 3517
NgoMIV G'CCGG_C
Cuts at: 3299 3299
Size: 5122
NruI TCG'CGA
Cuts at: 3483 3483
Size: 5122
NsiI A_TGCA'T
Cuts at: 783 783
Size: 5122
NspI r_CATG'y
Cuts at: 2173 2673 3582 2173
Size: 500 909 3713
Fragments arranged by size:
3713 909 500
PasI CC'CwG_GG
Cuts at: 306 840 306
Size: 534 4588
PciI A'CATG_T
Cuts at: 3578 3578
Size: 5122
PflMI CCAn_nnn'nTGG
Cuts at: 1034 1034
Size: 5122
PfoI T'CCnGG_A
Cuts at: 1810 1955 1810
Size: 145 4977
PmeI GTTT'AAAC
Cuts at: 2119 2119
Size: 5122
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4261 4293 4261
Size: 32 5090
PshAI GACnn'nnGTC
Cuts at: 2068 2068
Size: 5122
PspOMI G'GGCC_C
Cuts at: 836 836
Size: 5122
PspXI vC'TCGA_Gb
Cuts at: 962 962
Size: 5122
PstI C_TGCA'G
Cuts at: 222 299 1151 1938 2098 2167 2852 222
Size: 77 852 787 160 69 685 2492
Fragments arranged by size:
2492 852 787 685 160 77 69
PvuI CG_AT'CG
Cuts at: 104 1002 104
Size: 898 4224
PvuII CAG'CTG
Cuts at: 833 1925 2545 2905 833
Size: 1092 620 360 3050
Fragments arranged by size:
3050 1092 620 360
RsrII CG'GwC_CG
Cuts at: 3315 3315
Size: 5122
SacI G_AGCT'C
Cuts at: 2134 2134
Size: 5122
SalI G'TCGA_C
Cuts at: 2157 2157
Size: 5122
SapI GCTCTTCn'nnn_
Cuts at: 3143 3353 3143
Size: 210 4912
SbfI CC_TGCA'GG
Cuts at: 2167 2167
Size: 5122
ScaI AGT'ACT
Cuts at: 4639 4639
Size: 5122
SfcI C'TryA_G
Cuts at: 33 218 295 1147 1818 1934 2094 2163 2848
Size: 185 77 852 671 116 160 69 685
Cuts at: 2848 3843 4034 33
Size: 995 191 1121
Fragments arranged by size:
1121 995 852 685 671 191 185 160
116 77 69
SfoI GGC'GCC
Cuts at: 182 2552 2800 182
Size: 2370 248 2504
Fragments arranged by size:
2504 2370 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5122
SmaI CCC'GGG
Cuts at: 2142 2142
Size: 5122
SmlI C'TyrA_G
Cuts at: 962 2275 3190 3684 3946 962
Size: 1313 915 494 262 2138
Fragments arranged by size:
2138 1313 915 494 262
SnaBI TAC'GTA
Cuts at: 2112 2112
Size: 5122
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5122
SphI G_CATG'C
Cuts at: 2173 2673 2173
Size: 500 4622
SspI AAT'ATT
Cuts at: 699 699
Size: 5122
StyI C'CwwG_G
Cuts at: 125 1316 1730 2254 2608 125
Size: 1191 414 524 354 2639
Fragments arranged by size:
2639 1191 524 414 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 832 1029 2703 2836 3295 3506 832
Size: 197 1674 133 459 211 2448
Fragments arranged by size:
2448 1674 459 211 197 133
TatI w'GTAC_w
Cuts at: 4637 4637
Size: 5122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1120 1152 1660 1692 4639 4671 1120
Size: 32 508 32 2947 32 1571
Fragments arranged by size:
2947 1571 508 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2917 2917
Size: 5122
XbaI T'CTAG_A
Cuts at: 2151 2151
Size: 5122
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2615 2615
Size: 5122
XhoI C'TCGA_G
Cuts at: 962 962
Size: 5122
XmaI C'CCGG_G
Cuts at: 2140 2140
Size: 5122
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI AscI AvrII
BaeI BbvCI BglI BmgBI BmtI Bpu10I BsiWI BsmI
BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
PacI PmlI PpuMI PsiI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI StuI SwaI XmnI ZraI