Restriction map for
pF1KSDA0544
(Circular) (Six-base) MAPSORT of: pF1KSDA0544.seq Check: 3258 from: 1 to: 4795
pF1KSDA0544 4795 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1731 1731
Size: 4795
AccI GT'mk_AC
Cuts at: 569 1831 569
Size: 1262 3533
Acc65I G'GTAC_C
Cuts at: 1809 1809
Size: 4795
AclI AA'CG_TT
Cuts at: 4635 4635
Size: 4795
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 1131 2618 3050 3799 4067 293
Size: 838 1487 432 749 268 1021
Fragments arranged by size:
1487 1021 838 749 432 268
AflIII A'CryG_T
Cuts at: 2145 3251 4228 2145
Size: 1106 977 2712
Fragments arranged by size:
2712 1106 977
AgeI A'CCGG_T
Cuts at: 3183 3183
Size: 4795
AhdI GACnn_n'nnGTC
Cuts at: 1392 1392
Size: 4795
AlwNI CAG_nnn'CTG
Cuts at: 1170 2098 3667 1170
Size: 928 1569 2298
Fragments arranged by size:
2298 1569 928
ApaI G_GGCC'C
Cuts at: 1092 1754 1092
Size: 662 4133
ApaLI G'TGCA_C
Cuts at: 3565 3565
Size: 4795
ApoI r'AATT_y
Cuts at: 253 1797 4148 4404 253
Size: 1544 2351 256 644
Fragments arranged by size:
2351 1544 644 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4795
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4795
AvaI C'yCGr_G
Cuts at: 467 1300 1658 1699 1813 467
Size: 833 358 41 114 3449
Fragments arranged by size:
3449 833 358 114 41
BamHI G'GATC_C
Cuts at: 1818 1818
Size: 4795
BanI G'GyrC_C
Cuts at: 1809 2223 2471 2506 1809
Size: 414 248 35 4098
Fragments arranged by size:
4098 414 248 35
BanII G_rGCy'C
Cuts at: 133 365 586 1092 1131 1289 1754 1807 2837
Size: 232 221 506 39 158 465 53 1030
Cuts at: 2837 133
Size: 2091
Fragments arranged by size:
2091 1030 506 465 232 221 158 53
39
BbeI G_GCGC'C
Cuts at: 2227 2475 2227
Size: 248 4547
BbsI GAAGACnn'nnnn_
Cuts at: 376 1493 376
Size: 1117 3678
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 487 521 487
Size: 34 4761
BciVI GTATCCnnnnn_n'
Cuts at: 2691 3454 4795 2691
Size: 763 1341 2691
Fragments arranged by size:
2691 1341 763
BclI T'GATC_A
Cuts at: 2313 2313
Size: 4795
BglI GCCn_nnn'nGGC
Cuts at: 514 1228 1454 514
Size: 714 226 3855
Fragments arranged by size:
3855 714 226
BglII A'GATC_T
Cuts at: 881 2308 4347 881
Size: 1427 2039 1329
Fragments arranged by size:
2039 1427 1329
BlpI GC'TnA_GC
Cuts at: 1905 1905
Size: 4795
Bme1580I G_kGCm'C
Cuts at: 1092 1754 2418 2511 3569 1092
Size: 662 664 93 1058 2318
Fragments arranged by size:
2318 1058 664 662 93
BmgBI CAC'GTC
Cuts at: 1545 1545
Size: 4795
BmrI ACTGGGnnnn_n'
Cuts at: 1573 2005 2086 2421 4567 1573
Size: 432 81 335 2146 1801
Fragments arranged by size:
2146 1801 432 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1308 1551 3160 1308
Size: 243 1609 2943
Fragments arranged by size:
2943 1609 243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 1192 505
Size: 687 4108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1969 2848 3342 3640 1969
Size: 879 494 298 3124
Fragments arranged by size:
3124 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 595 907 4499 595
Size: 312 3592 891
Fragments arranged by size:
3592 891 312
BsaAI yAC'GTr
Cuts at: 780 1785 2146 780
Size: 1005 361 3429
Fragments arranged by size:
3429 1005 361
BsaBI GATnn'nnATC
Cuts at: 6 2307 4346 6
Size: 2301 2039 455
Fragments arranged by size:
2301 2039 455
BsaHI Gr'CG_yC
Cuts at: 500 1550 2224 2472 3176 4672 500
Size: 1050 674 248 704 1496 623
Fragments arranged by size:
1496 1050 704 674 623 248
BsaWI w'CCGG_w
Cuts at: 211 1982 2503 3183 3457 3604 4015 211
Size: 1771 521 680 274 147 411 991
Fragments arranged by size:
1771 991 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 740 770 1207 1237 740
Size: 30 437 30 4298
Fragments arranged by size:
4298 437 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 260 559 1540 260
Size: 299 981 3515
Fragments arranged by size:
3515 981 299
BseYI C'CCAG_C
Cuts at: 2218 2976 3555 4051 2218
Size: 758 579 496 2962
Fragments arranged by size:
2962 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 2335 2381 3591 104
Size: 2231 46 1210 1308
Fragments arranged by size:
2231 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 476 785 1187 1706 1807 2585 2775 3569 476
Size: 309 402 519 101 778 190 794 1702
Fragments arranged by size:
1702 794 778 519 402 309 190 101
Bsp1286I G_dGCh'C
Cuts at: 133 365 476 586 785 1092 1131 1187 1289
Size: 232 111 110 199 307 39 56 102
Cuts at: 1289 1706 1754 1807 2418 2511 2585 2775 2837
Size: 417 48 53 611 93 74 190 62
Cuts at: 2837 3569 133
Size: 732 1359
Fragments arranged by size:
1359 732 611 417 307 232 199 190
111 110 102 93 74 62 56 53
48 39
BspEI T'CCGG_A
Cuts at: 211 4015 211
Size: 3804 991
BspHI T'CATG_A
Cuts at: 3971 3971
Size: 4795
BspMI ACCTGCnnnn'nnnn_
Cuts at: 986 1731 1745 1843 2057 2359 2740 986
Size: 745 14 98 214 302 381 3041
Fragments arranged by size:
3041 745 381 302 214 98 14
BsrBI CCG'CTC
Cuts at: 50 3085 3139 4625 4790 50
Size: 3035 54 1486 165 55
Fragments arranged by size:
3035 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2705 2705
Size: 4795
BsrFI r'CCGG_y
Cuts at: 1073 2791 2972 3183 1073
Size: 1718 181 211 2685
Fragments arranged by size:
2685 1718 211 181
BssSI C'ACGA_G
Cuts at: 358 3064 3424 358
Size: 2706 360 1729
Fragments arranged by size:
2706 1729 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2170 2170
Size: 4795
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4795
BstEII G'GTnAC_C
Cuts at: 277 277
Size: 4795
BstYI r'GATC_y
Cuts at: 1 300 881 1100 1818 2285 2308 2643 2889
Size: 299 581 219 718 467 23 335 246
Cuts at: 2889 3903 3989 4001 4347 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 718 581 467 449 346 335 299
246 219 86 23 12
BstZ17I GTA'TAC
Cuts at: 570 570
Size: 4795
BtgI C'CryG_G
Cuts at: 125 125
Size: 4795
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 135 2743 2924 3087 135
Size: 2608 181 163 1843
Fragments arranged by size:
2608 1843 181 163
BtsI GCAGTG_nn'
Cuts at: 2036 2036
Size: 4795
DraI TTT'AAA
Cuts at: 836 1200 1792 836
Size: 364 592 3839
Fragments arranged by size:
3839 592 364
DraIII CAC_nnn'GTG
Cuts at: 1567 1567
Size: 4795
DrdI GACnn_nn'nnGTC
Cuts at: 2499 3359 2499
Size: 860 3935
EaeI y'GGCC_r
Cuts at: 515 768 1229 1334 1710 2378 2552 2943 2970
Size: 253 461 105 376 668 174 391 27
Cuts at: 2970 4317 515
Size: 1347 993
Fragments arranged by size:
1347 993 668 461 391 376 253 174
105 27
EagI C'GGCC_G
Cuts at: 2378 2378
Size: 4795
EarI CTCTTCn'nnn_
Cuts at: 1511 1616 2816 3026 1511
Size: 105 1200 210 3280
Fragments arranged by size:
3280 1200 210 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3001 3313 3459 4605 3001
Size: 312 146 1146 3191
Fragments arranged by size:
3191 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1805 1805
Size: 4795
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 505 1131 1192 2618 3050 3799 4067 293
Size: 212 626 61 1426 432 749 268 1021
Fragments arranged by size:
1426 1021 749 626 432 268 212 61
EcoNI CCTnn'n_nnAGG
Cuts at: 874 1396 874
Size: 522 4273
EcoO109I rG'GnC_Cy
Cuts at: 1089 1473 1655 1750 1932 1089
Size: 384 182 95 182 3952
Fragments arranged by size:
3952 384 182 182 95
EcoRI G'AATT_C
Cuts at: 1797 1797
Size: 4795
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 678 2812 678
Size: 2134 2661
FspI TGC'GCA
Cuts at: 2574 2574
Size: 4795
HaeII r_GCGC'y
Cuts at: 1309 2227 2475 3499 4478 1309
Size: 918 248 1024 979 1626
Fragments arranged by size:
1626 1024 979 918 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1171 1203 1378 1410 1621 1624 1653 1656 1747
Size: 32 175 32 211 3 29 3 91
Cuts at: 1747 1779 2635 2665 2667 2697 1171
Size: 32 856 30 2 30 3269
Fragments arranged by size:
3269 856 211 175 91 32 32 32
30 30 29 3 3 2
HincII GTy'rAC
Cuts at: 1832 1832
Size: 4795
KasI G'GCGC_C
Cuts at: 2223 2471 2223
Size: 248 4547
KpnI G_GTAC'C
Cuts at: 1813 1813
Size: 4795
MluI A'CGCG_T
Cuts at: 4228 4228
Size: 4795
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 998 3466 3650 998
Size: 2468 184 2143
Fragments arranged by size:
2468 2143 184
MscI TGG'CCA
Cuts at: 517 770 1231 2554 517
Size: 253 461 1323 2758
Fragments arranged by size:
2758 1323 461 253
MslI CAynn'nnrTG
Cuts at: 2782 2909 2782
Size: 127 4668
MspA1I CmG'CkG
Cuts at: 1406 1904 2218 2578 3593 3838 1406
Size: 498 314 360 1015 245 2363
Fragments arranged by size:
2363 1015 498 360 314 245
NaeI GCC'GGC
Cuts at: 1075 2974 1075
Size: 1899 2896
NarI GG'CG_CC
Cuts at: 2224 2472 2224
Size: 248 4547
NcoI C'CATG_G
Cuts at: 125 125
Size: 4795
NgoMIV G'CCGG_C
Cuts at: 1073 2972 1073
Size: 1899 2896
NruI TCG'CGA
Cuts at: 3156 3156
Size: 4795
NspI r_CATG'y
Cuts at: 1846 2346 3255 1846
Size: 500 909 3386
Fragments arranged by size:
3386 909 500
PasI CC'CwG_GG
Cuts at: 1746 1746
Size: 4795
PciI A'CATG_T
Cuts at: 3251 3251
Size: 4795
PflMI CCAn_nnn'nTGG
Cuts at: 735 1170 1434 1567 735
Size: 435 264 133 3963
Fragments arranged by size:
3963 435 264 133
PfoI T'CCnGG_A
Cuts at: 1433 1433
Size: 4795
PmeI GTTT'AAAC
Cuts at: 1792 1792
Size: 4795
PmlI CAC'GTG
Cuts at: 780 780
Size: 4795
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3934 3966 3934
Size: 32 4763
PpuMI rG'GwC_Cy
Cuts at: 1655 1655
Size: 4795
PspOMI G'GGCC_C
Cuts at: 1088 1750 1088
Size: 662 4133
PspXI vC'TCGA_Gb
Cuts at: 1300 1300
Size: 4795
PstI C_TGCA'G
Cuts at: 878 1100 1688 1840 2525 878
Size: 222 588 152 685 3148
Fragments arranged by size:
3148 685 588 222 152
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4795
PvuII CAG'CTG
Cuts at: 1406 2218 2578 1406
Size: 812 360 3623
Fragments arranged by size:
3623 812 360
RsrII CG'GwC_CG
Cuts at: 2988 2988
Size: 4795
SacI G_AGCT'C
Cuts at: 1807 1807
Size: 4795
SalI G'TCGA_C
Cuts at: 1830 1830
Size: 4795
SanDI GG'GwC_CC
Cuts at: 1655 1655
Size: 4795
SapI GCTCTTCn'nnn_
Cuts at: 2816 3026 2816
Size: 210 4585
SbfI CC_TGCA'GG
Cuts at: 878 1688 1840 878
Size: 810 152 3833
Fragments arranged by size:
3833 810 152
ScaI AGT'ACT
Cuts at: 4312 4312
Size: 4795
SfcI C'TryA_G
Cuts at: 33 477 592 874 1096 1684 1836 2521 3516
Size: 444 115 282 222 588 152 685 995
Cuts at: 3516 3707 33
Size: 191 1121
Fragments arranged by size:
1121 995 685 588 444 282 222 191
152 115
SfoI GGC'GCC
Cuts at: 2225 2473 2225
Size: 248 4547
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4795
SmaI CCC'GGG
Cuts at: 469 1815 469
Size: 1346 3449
SmlI C'TyrA_G
Cuts at: 1300 1948 2863 3357 3619 1300
Size: 648 915 494 262 2476
Fragments arranged by size:
2476 915 648 494 262
SnaBI TAC'GTA
Cuts at: 1785 1785
Size: 4795
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4795
SphI G_CATG'C
Cuts at: 1846 2346 1846
Size: 500 4295
StyI C'CwwG_G
Cuts at: 125 1927 2281 125
Size: 1802 354 2639
Fragments arranged by size:
2639 1802 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1555 2376 2509 2968 3179 1555
Size: 821 133 459 211 3171
Fragments arranged by size:
3171 821 459 211 133
TatI w'GTAC_w
Cuts at: 4310 4310
Size: 4795
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 740 772 1207 1239 4312 4344 740
Size: 32 435 32 3073 32 1191
Fragments arranged by size:
3073 1191 435 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 859 1267 2590 859
Size: 408 1323 3064
Fragments arranged by size:
3064 1323 408
XbaI T'CTAG_A
Cuts at: 1824 1824
Size: 4795
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2288 2288
Size: 4795
XhoI C'TCGA_G
Cuts at: 1300 1300
Size: 4795
XmaI C'CCGG_G
Cuts at: 467 1813 467
Size: 1346 3449
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmtI Bpu10I BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstXI Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PshAI PsiI PsrI SacII SexAI SfiI
SgrAI SrfI SspI StuI SwaI XmnI ZraI