Restriction map for
pF1KSDA0550
(Circular) (Six-base) MAPSORT of: pF1KSDA0550.seq Check: 1501 from: 1 to: 7703
pF1KSDA0550 7703 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 724 1050 1888 2142 724
Size: 326 838 254 6285
Fragments arranged by size:
6285 838 326 254
AccI GT'mk_AC
Cuts at: 703 3701 4741 703
Size: 2998 1040 3665
Fragments arranged by size:
3665 2998 1040
Acc65I G'GTAC_C
Cuts at: 4719 4719
Size: 7703
AclI AA'CG_TT
Cuts at: 7543 7543
Size: 7703
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2741 3842 5528 5960 6707 6975 2741
Size: 1101 1686 432 747 268 3469
Fragments arranged by size:
3469 1686 1101 747 432 268
AflIII A'CryG_T
Cuts at: 1030 1204 5055 6159 7136 1030
Size: 174 3851 1104 977 1597
Fragments arranged by size:
3851 1597 1104 977 174
AgeI A'CCGG_T
Cuts at: 6091 6091
Size: 7703
AhdI GACnn_n'nnGTC
Cuts at: 753 753
Size: 7703
AleI CACnn'nnGTG
Cuts at: 2368 4273 2368
Size: 1905 5798
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1424 3618 1424
Size: 2194 5509
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1017 1049 1179 1211 1509 1541 1017
Size: 32 130 32 298 32 7179
Fragments arranged by size:
7179 298 130 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 759 825 1769 2263 3217 5008 6575 759
Size: 66 944 494 954 1791 1567 1887
Fragments arranged by size:
1887 1791 1567 954 944 494 66
ApaLI G'TGCA_C
Cuts at: 1404 6473 1404
Size: 5069 2634
ApoI r'AATT_y
Cuts at: 340 501 883 976 1953 2205 4168 4248 4281
Size: 161 382 93 977 252 1963 80 33
Cuts at: 4281 4456 4647 4707 7056 7312 340
Size: 175 191 60 2349 256 731
Fragments arranged by size:
2349 1963 977 731 382 256 252 191
175 161 93 80 60 33
AseI AT'TA_AT
Cuts at: 20 2474 20
Size: 2454 5249
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7703
AvaI C'yCGr_G
Cuts at: 4723 4723
Size: 7703
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1064 1097 1064
Size: 33 7670
BamHI G'GATC_C
Cuts at: 3624 4728 3624
Size: 1104 6599
BanI G'GyrC_C
Cuts at: 1753 4719 5133 5381 5416 1753
Size: 2966 414 248 35 4040
Fragments arranged by size:
4040 2966 414 248 35
BanII G_rGCy'C
Cuts at: 3430 4717 5747 3430
Size: 1287 1030 5386
Fragments arranged by size:
5386 1287 1030
BbeI G_GCGC'C
Cuts at: 5137 5385 5137
Size: 248 7455
BbsI GAAGACnn'nnnn_
Cuts at: 1774 1774
Size: 7703
BbvCI CC'TCA_GC
Cuts at: 722 722
Size: 7703
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3626 3660 3626
Size: 34 7669
BciVI GTATCCnnnnn_n'
Cuts at: 5601 6362 7703 5601
Size: 761 1341 5601
Fragments arranged by size:
5601 1341 761
BclI T'GATC_A
Cuts at: 396 3153 5223 396
Size: 2757 2070 2876
Fragments arranged by size:
2876 2757 2070
BfrBI ATG'CAT
Cuts at: 1826 2662 3595 4410 1826
Size: 836 933 815 5119
Fragments arranged by size:
5119 933 836 815
BglI GCCn_nnn'nGGC
Cuts at: 2710 2710
Size: 7703
BglII A'GATC_T
Cuts at: 2436 5218 7255 2436
Size: 2782 2037 2884
Fragments arranged by size:
2884 2782 2037
BlpI GC'TnA_GC
Cuts at: 2714 3829 3919 4815 2714
Size: 1115 90 896 5602
Fragments arranged by size:
5602 1115 896 90
Bme1580I G_kGCm'C
Cuts at: 1408 5328 5421 6477 1408
Size: 3920 93 1056 2634
Fragments arranged by size:
3920 2634 1056 93
BmgBI CAC'GTC
Cuts at: 3461 3461
Size: 7703
BmrI ACTGGGnnnn_n'
Cuts at: 1014 1312 2325 4547 4915 4996 5331 7475 1014
Size: 298 1013 2222 368 81 335 2144 1242
Fragments arranged by size:
2222 2144 1242 1013 368 335 298 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3205 3344 6070 3205
Size: 139 2726 4838
Fragments arranged by size:
4838 2726 139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1691 2554 1691
Size: 863 6840
Bpu10I CC'TnA_GC
Cuts at: 358 722 1877 358
Size: 364 1155 6184
Fragments arranged by size:
6184 1155 364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4007 4879 5758 6250 6548 4007
Size: 872 879 492 298 5162
Fragments arranged by size:
5162 879 872 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 7407 7407
Size: 7703
BsaAI yAC'GTr
Cuts at: 4695 5056 4695
Size: 361 7342
BsaBI GATnn'nnATC
Cuts at: 6 1827 5217 7254 6
Size: 1821 3390 2037 455
Fragments arranged by size:
3390 2037 1821 455
BsaHI Gr'CG_yC
Cuts at: 3415 5134 5382 6084 7580 3415
Size: 1719 248 702 1496 3538
Fragments arranged by size:
3538 1719 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2367 4892 5413 6091 6365 6512 6923 2367
Size: 2525 521 678 274 147 411 3147
Fragments arranged by size:
3147 2525 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1017 1047 1162 1179 1192 1209 1509 1539 3417
Size: 30 115 17 13 17 300 30 1878
Cuts at: 3417 3447 1017
Size: 30 5273
Fragments arranged by size:
5273 1878 300 115 30 30 30 17
17 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1238 1238
Size: 7703
BseYI C'CCAG_C
Cuts at: 1547 5128 5886 6463 6959 1547
Size: 3581 758 577 496 2291
Fragments arranged by size:
3581 2291 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 1598 1634 2931 273
Size: 1325 36 1297 5045
Fragments arranged by size:
5045 1325 1297 36
BsiEI CG_ry'CG
Cuts at: 104 5245 5291 6499 104
Size: 5141 46 1208 1308
Fragments arranged by size:
5141 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1030 1363 1375 1408 1871 3430 4717 5495 5685
Size: 333 12 33 463 1559 1287 778 190
Cuts at: 5685 6477 1030
Size: 792 2256
Fragments arranged by size:
2256 1559 1287 792 778 463 333 190
33 12
BsmI GAATG_Cn'
Cuts at: 477 1438 1723 1749 1854 1895 3569 477
Size: 961 285 26 105 41 1674 4611
Fragments arranged by size:
4611 1674 961 285 105 41 26
BsmBI CGTCTCn'nnnn_
Cuts at: 2695 3422 2695
Size: 727 6976
Bsp1286I G_dGCh'C
Cuts at: 1030 1363 1375 1408 1871 3430 4717 5328 5421
Size: 333 12 33 463 1559 1287 611 93
Cuts at: 5421 5495 5685 5747 6477 1030
Size: 74 190 62 730 2256
Fragments arranged by size:
2256 1559 1287 730 611 463 333 190
93 74 62 33 12
BspEI T'CCGG_A
Cuts at: 6923 6923
Size: 7703
BspHI T'CATG_A
Cuts at: 936 3677 6879 936
Size: 2741 3202 1760
Fragments arranged by size:
3202 2741 1760
BspMI ACCTGCnnnn'nnnn_
Cuts at: 152 724 1050 1888 2142 3216 4753 4967 5269
Size: 572 326 838 254 1074 1537 214 302
Cuts at: 5269 5650 152
Size: 381 2205
Fragments arranged by size:
2205 1537 1074 838 572 381 326 302
254 214
BsrBI CCG'CTC
Cuts at: 50 5995 6049 7533 7698 50
Size: 5945 54 1484 165 55
Fragments arranged by size:
5945 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1603 3535 3704 3841 5615 1603
Size: 1932 169 137 1774 3691
Fragments arranged by size:
3691 1932 1774 169 137
BsrFI r'CCGG_y
Cuts at: 1397 3310 5701 5882 6091 1397
Size: 1913 2391 181 209 3009
Fragments arranged by size:
3009 2391 1913 209 181
BsrGI T'GTAC_A
Cuts at: 1480 1480
Size: 7703
BssSI C'ACGA_G
Cuts at: 5974 6332 5974
Size: 358 7345
BstAPI GCAn_nnn'nTGC
Cuts at: 1463 1769 1890 4251 4606 5080 1463
Size: 306 121 2361 355 474 4086
Fragments arranged by size:
4086 2361 474 355 306 121
BstBI TT'CG_AA
Cuts at: 110 6064 110
Size: 5954 1749
BstEII G'GTnAC_C
Cuts at: 1178 1917 1178
Size: 739 6964
BstXI CCAn_nnnn'nTGG
Cuts at: 4631 4631
Size: 7703
BstYI r'GATC_y
Cuts at: 1 312 423 1988 2436 2854 3624 4728 5195
Size: 311 111 1565 448 418 770 1104 467
Cuts at: 5195 5218 5553 5799 6811 6897 6909 7255 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1565 1104 1012 770 467 449 448 418
346 335 311 246 111 86 23 12
BstZ17I GTA'TAC
Cuts at: 704 704
Size: 7703
Bsu36I CC'TnA_GG
Cuts at: 953 1670 953
Size: 717 6986
BtgI C'CryG_G
Cuts at: 1183 1419 1444 3410 4531 1183
Size: 236 25 1966 1121 4355
Fragments arranged by size:
4355 1966 1121 236 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1656 5653 5834 5997 1656
Size: 3997 181 163 3362
Fragments arranged by size:
3997 3362 181 163
BtsI GCAGTG_nn'
Cuts at: 1098 1139 1406 1408 1586 1638 2784 2957 3564
Size: 41 267 2 178 52 1146 173 607
Cuts at: 3564 4946 1098
Size: 1382 3855
Fragments arranged by size:
3855 1382 1146 607 267 178 173 52
41 2
DraI TTT'AAA
Cuts at: 2016 4702 2016
Size: 2686 5017
DrdI GACnn_nn'nnGTC
Cuts at: 3465 5409 6267 3465
Size: 1944 858 4901
Fragments arranged by size:
4901 1944 858
EaeI y'GGCC_r
Cuts at: 3110 3479 5288 5462 5853 5880 7225 3110
Size: 369 1809 174 391 27 1345 3588
Fragments arranged by size:
3588 1809 1345 391 369 174 27
EagI C'GGCC_G
Cuts at: 5288 5288
Size: 7703
EarI CTCTTCn'nnn_
Cuts at: 1004 4414 5726 5936 1004
Size: 3410 1312 210 2771
Fragments arranged by size:
3410 2771 1312 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5911 6221 6367 7513 5911
Size: 310 146 1146 6101
Fragments arranged by size:
6101 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3428 4715 3428
Size: 1287 6416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1691 2554 2741 3842 5528 5960 6707 6975 1691
Size: 863 187 1101 1686 432 747 268 2419
Fragments arranged by size:
2419 1686 1101 863 747 432 268 187
EcoNI CCTnn'n_nnAGG
Cuts at: 817 1876 817
Size: 1059 6644
EcoO109I rG'GnC_Cy
Cuts at: 354 948 1276 1565 3205 4842 354
Size: 594 328 289 1640 1637 3215
Fragments arranged by size:
3215 1640 1637 594 328 289
EcoRI G'AATT_C
Cuts at: 2205 4168 4707 2205
Size: 1963 539 5201
Fragments arranged by size:
5201 1963 539
EcoRV GAT'ATC
Cuts at: 4346 4346
Size: 7703
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1888 5722 1888
Size: 3834 3869
FspI TGC'GCA
Cuts at: 5484 5484
Size: 7703
HaeII r_GCGC'y
Cuts at: 5137 5385 6407 7386 5137
Size: 248 1022 979 5454
Fragments arranged by size:
5454 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 559 591 739 771 885 917 1162 1194 1894
Size: 32 148 32 114 32 245 32 700
Cuts at: 1894 1926 2380 2412 2677 2709 2717 2749 3266
Size: 32 454 32 265 32 8 32 517
Cuts at: 3266 3298 3481 3513 3603 3635 5545 5575 5577
Size: 32 183 32 90 32 1910 30 2
Cuts at: 5577 5607 559
Size: 30 2655
Fragments arranged by size:
2655 1910 700 517 454 265 245 183
148 114 90 32 32 32 32 32
32 32 32 32 32 32 30 30
8 2
HincII GTy'rAC
Cuts at: 2313 3231 4742 2313
Size: 918 1511 5274
Fragments arranged by size:
5274 1511 918
HindIII A'AGCT_T
Cuts at: 2248 2248
Size: 7703
KasI G'GCGC_C
Cuts at: 5133 5381 5133
Size: 248 7455
KpnI G_GTAC'C
Cuts at: 4723 4723
Size: 7703
MluI A'CGCG_T
Cuts at: 7136 7136
Size: 7703
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 340 553 629 1913 2160 2895 4280 6374 6558
Size: 213 76 1284 247 735 1385 2094 184
Cuts at: 6558 340
Size: 1485
Fragments arranged by size:
2094 1485 1385 1284 735 247 213 184
76
MscI TGG'CCA
Cuts at: 3112 3481 5464 3112
Size: 369 1983 5351
Fragments arranged by size:
5351 1983 369
MslI CAynn'nnrTG
Cuts at: 1203 1517 2368 3600 3901 3907 4273 5692 5819
Size: 314 851 1232 301 6 366 1419 127
Cuts at: 5819 1203
Size: 3087
Fragments arranged by size:
3087 1419 1232 851 366 314 301 127
6
MspA1I CmG'CkG
Cuts at: 1106 1414 3217 4273 4814 5128 5488 6501 6746
Size: 308 1803 1056 541 314 360 1013 245
Cuts at: 6746 1106
Size: 2063
Fragments arranged by size:
2063 1803 1056 1013 541 360 314 308
245
NaeI GCC'GGC
Cuts at: 1399 5884 1399
Size: 4485 3218
NarI GG'CG_CC
Cuts at: 5134 5382 5134
Size: 248 7455
NcoI C'CATG_G
Cuts at: 1183 1419 1444 3410 4531 1183
Size: 236 25 1966 1121 4355
Fragments arranged by size:
4355 1966 1121 236 25
NdeI CA'TA_TG
Cuts at: 4241 4427 4241
Size: 186 7517
NgoMIV G'CCGG_C
Cuts at: 1397 5882 1397
Size: 4485 3218
NsiI A_TGCA'T
Cuts at: 1828 2664 3597 4412 1828
Size: 836 933 815 5119
Fragments arranged by size:
5119 933 836 815
NspI r_CATG'y
Cuts at: 1034 1208 3670 3900 4756 5256 6163 1034
Size: 174 2462 230 856 500 907 2574
Fragments arranged by size:
2574 2462 907 856 500 230 174
PasI CC'CwG_GG
Cuts at: 2634 2634
Size: 7703
PciI A'CATG_T
Cuts at: 1030 1204 6159 1030
Size: 174 4955 2574
Fragments arranged by size:
4955 2574 174
PflMI CCAn_nnn'nTGG
Cuts at: 1324 1324
Size: 7703
PmeI GTTT'AAAC
Cuts at: 4702 4702
Size: 7703
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1017 1049 1179 1211 1509 1541 6842 6874 1017
Size: 32 130 32 298 32 5301 32 1846
Fragments arranged by size:
5301 1846 298 130 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 354 948 1276 1565 3205 354
Size: 594 328 289 1640 4852
Fragments arranged by size:
4852 1640 594 328 289
PsiI TTA'TAA
Cuts at: 3061 3061
Size: 7703
PstI C_TGCA'G
Cuts at: 1105 1413 2259 3213 4750 5435 1105
Size: 308 846 954 1537 685 3373
Fragments arranged by size:
3373 1537 954 846 685 308
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7703
PvuII CAG'CTG
Cuts at: 1414 4273 5128 5488 1414
Size: 2859 855 360 3629
Fragments arranged by size:
3629 2859 855 360
RsrII CG'GwC_CG
Cuts at: 5898 5898
Size: 7703
SacI G_AGCT'C
Cuts at: 3430 4717 3430
Size: 1287 6416
SalI G'TCGA_C
Cuts at: 4740 4740
Size: 7703
SanDI GG'GwC_CC
Cuts at: 948 948
Size: 7703
SapI GCTCTTCn'nnn_
Cuts at: 5726 5936 5726
Size: 210 7493
SbfI CC_TGCA'GG
Cuts at: 4750 4750
Size: 7703
ScaI AGT'ACT
Cuts at: 7220 7220
Size: 7703
SfcI C'TryA_G
Cuts at: 33 487 1101 1409 2255 3209 4746 5431 6424
Size: 454 614 308 846 954 1537 685 993
Cuts at: 6424 6615 33
Size: 191 1121
Fragments arranged by size:
1537 1121 993 954 846 685 614 454
308 191
SfoI GGC'GCC
Cuts at: 5135 5383 5135
Size: 248 7455
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7703
SmaI CCC'GGG
Cuts at: 4725 4725
Size: 7703
SmlI C'TyrA_G
Cuts at: 4022 4858 5773 6265 6527 4022
Size: 836 915 492 262 5198
Fragments arranged by size:
5198 915 836 492 262
SnaBI TAC'GTA
Cuts at: 4695 4695
Size: 7703
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7703
SphI G_CATG'C
Cuts at: 3670 4756 5256 3670
Size: 1086 500 6117
Fragments arranged by size:
6117 1086 500
SspI AAT'ATT
Cuts at: 1305 4113 1305
Size: 2808 4895
StuI AGG'CCT
Cuts at: 2505 2505
Size: 7703
StyI C'CwwG_G
Cuts at: 1183 1419 1444 3081 3410 4531 4837 5191 1183
Size: 236 25 1637 329 1121 306 354 3695
Fragments arranged by size:
3695 1637 1121 354 329 306 236 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 850 888 2647 5286 5419 5878 6087 850
Size: 38 1759 2639 133 459 209 2466
Fragments arranged by size:
2639 2466 1759 459 209 133 38
TatI w'GTAC_w
Cuts at: 640 1155 1480 1838 3968 7218 640
Size: 515 325 358 2130 3250 1125
Fragments arranged by size:
3250 2130 1125 515 358 325
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2216 2248 3417 3449 7220 7252 2216
Size: 32 1169 32 3771 32 2667
Fragments arranged by size:
3771 2667 1169 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4012 5500 4012
Size: 1488 6215
XbaI T'CTAG_A
Cuts at: 2082 4734 2082
Size: 2652 5051
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 171 1929 2487 3408 5198 171
Size: 1758 558 921 1790 2676
Fragments arranged by size:
2676 1790 1758 921 558
XmaI C'CCGG_G
Cuts at: 4723 4723
Size: 7703
XmnI GAAnn'nnTTC
Cuts at: 266 475 3771 4282 4436 266
Size: 209 3296 511 154 3533
Fragments arranged by size:
3533 3296 511 209 154
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII ApaI AscI AvrII BmtI BsiWI
BssHII ClaI CspCI DraIII FseI FspAI HpaI MfeI
NheI NotI NruI PacI PfoI PmlI PshAI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SrfI SwaI
XhoI ZraI