Restriction map for
pF1KSDA0557
(Circular) (Six-base) MAPSORT of: pF1KSDA0557.seq Check: 119 from: 1 to: 4577
pF1KSDA0557 4577 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1111 1447 1111
Size: 336 4241
AatII G_ACGT'C
Cuts at: 1138 1138
Size: 4577
AccI GT'mk_AC
Cuts at: 818 1615 818
Size: 797 3780
Acc65I G'GTAC_C
Cuts at: 950 1593 950
Size: 643 3934
AclI AA'CG_TT
Cuts at: 4417 4417
Size: 4577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1110 1212 1278 1380 2402 2834 3581 3849 1110
Size: 102 66 102 1022 432 747 268 1838
Fragments arranged by size:
1838 1022 747 432 268 102 102 66
AfeI AGC'GCT
Cuts at: 1291 1375 1291
Size: 84 4493
AflIII A'CryG_T
Cuts at: 1929 3033 4010 1929
Size: 1104 977 2496
Fragments arranged by size:
2496 1104 977
AgeI A'CCGG_T
Cuts at: 2965 2965
Size: 4577
AleI CACnn'nnGTG
Cuts at: 1421 1421
Size: 4577
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1495 1495
Size: 4577
AlwNI CAG_nnn'CTG
Cuts at: 153 174 393 420 1882 3449 153
Size: 21 219 27 1462 1567 1281
Fragments arranged by size:
1567 1462 1281 219 27 21
ApaI G_GGCC'C
Cuts at: 317 703 317
Size: 386 4191
ApaLI G'TGCA_C
Cuts at: 1246 3347 1246
Size: 2101 2476
ApoI r'AATT_y
Cuts at: 1581 3930 4186 1581
Size: 2349 256 1972
Fragments arranged by size:
2349 1972 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4577
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
AvaI C'yCGr_G
Cuts at: 317 449 574 1276 1597 317
Size: 132 125 702 321 3297
Fragments arranged by size:
3297 702 321 132 125
BamHI G'GATC_C
Cuts at: 893 1602 893
Size: 709 3868
BanI G'GyrC_C
Cuts at: 567 789 950 1471 1593 2007 2255 2290 567
Size: 222 161 521 122 414 248 35 2854
Fragments arranged by size:
2854 521 414 248 222 161 122 35
BanII G_rGCy'C
Cuts at: 236 317 544 703 1306 1591 2621 236
Size: 81 227 159 603 285 1030 2192
Fragments arranged by size:
2192 1030 603 285 227 159 81
BbeI G_GCGC'C
Cuts at: 2011 2259 2011
Size: 248 4329
BbsI GAAGACnn'nnnn_
Cuts at: 1074 1074
Size: 4577
BciVI GTATCCnnnnn_n'
Cuts at: 2475 3236 4577 2475
Size: 761 1341 2475
Fragments arranged by size:
2475 1341 761
BclI T'GATC_A
Cuts at: 1038 2097 1038
Size: 1059 3518
BfrBI ATG'CAT
Cuts at: 845 845
Size: 4577
BglII A'GATC_T
Cuts at: 2092 4129 2092
Size: 2037 2540
BlpI GC'TnA_GC
Cuts at: 614 679 857 1689 614
Size: 65 178 832 3502
Fragments arranged by size:
3502 832 178 65
Bme1580I G_kGCm'C
Cuts at: 317 572 703 794 1250 1276 1474 2202 2295
Size: 255 131 91 456 26 198 728 93
Cuts at: 2295 3351 317
Size: 1056 1543
Fragments arranged by size:
1543 1056 728 456 255 198 131 93
91 26
BmgBI CAC'GTC
Cuts at: 810 810
Size: 4577
BmrI ACTGGGnnnn_n'
Cuts at: 1009 1356 1789 1870 2205 4349 1009
Size: 347 433 81 335 2144 1237
Fragments arranged by size:
2144 1237 433 347 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 252 743 2944 252
Size: 491 2201 1885
Fragments arranged by size:
2201 1885 491
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 415 430 639 655 713 895 1290 1513
Size: 285 15 209 16 58 182 395 223
Cuts at: 1513 1565 130
Size: 52 3142
Fragments arranged by size:
3142 395 285 223 209 182 58 52
16 15
Bpu10I CC'TnA_GC
Cuts at: 328 328
Size: 4577
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1753 2632 3124 3422 1753
Size: 879 492 298 2908
Fragments arranged by size:
2908 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4281 4281
Size: 4577
BsaAI yAC'GTr
Cuts at: 1569 1930 1569
Size: 361 4216
BsaBI GATnn'nnATC
Cuts at: 6 2091 4128 6
Size: 2085 2037 455
Fragments arranged by size:
2085 2037 455
BsaHI Gr'CG_yC
Cuts at: 754 1135 2008 2256 2958 4454 754
Size: 381 873 248 702 1496 877
Fragments arranged by size:
1496 877 873 702 381 248
BsaWI w'CCGG_w
Cuts at: 1766 2287 2965 3239 3386 3797 1766
Size: 521 678 274 147 411 2546
Fragments arranged by size:
2546 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 739 769 739
Size: 30 4547
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 244 496 847 223
Size: 21 252 351 3953
Fragments arranged by size:
3953 351 252 21
BseYI C'CCAG_C
Cuts at: 256 310 669 674 852 2002 2760 3337 3833
Size: 54 359 5 178 1150 758 577 496
Cuts at: 3833 256
Size: 1000
Fragments arranged by size:
1150 1000 758 577 496 359 178 54
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1341 1465 1341
Size: 124 4453
BsiEI CG_ry'CG
Cuts at: 104 367 1218 2119 2165 3373 104
Size: 263 851 901 46 1208 1308
Fragments arranged by size:
1308 1208 901 851 263 46
BsiHKAI G_wGCw'C
Cuts at: 962 1250 1306 1591 2369 2559 3351 962
Size: 288 56 285 778 190 792 2188
Fragments arranged by size:
2188 792 778 288 285 190 56
BsmBI CGTCTCn'nnnn_
Cuts at: 238 238
Size: 4577
Bsp1286I G_dGCh'C
Cuts at: 236 317 544 572 703 794 962 1250 1276
Size: 81 227 28 131 91 168 288 26
Cuts at: 1276 1306 1474 1591 2202 2295 2369 2559 2621
Size: 30 168 117 611 93 74 190 62
Cuts at: 2621 3351 236
Size: 730 1462
Fragments arranged by size:
1462 730 611 288 227 190 168 168
131 117 93 91 81 74 62 30
28 26
BspEI T'CCGG_A
Cuts at: 3797 3797
Size: 4577
BspHI T'CATG_A
Cuts at: 3753 3753
Size: 4577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1111 1447 1487 1627 1841 2143 2524 1111
Size: 336 40 140 214 302 381 3164
Fragments arranged by size:
3164 381 336 302 214 140 40
BsrBI CCG'CTC
Cuts at: 50 476 993 1176 1220 1344 2869 2923 4407
Size: 426 517 183 44 124 1525 54 1484
Cuts at: 4407 4572 50
Size: 165 55
Fragments arranged by size:
1525 1484 517 426 183 165 124 55
54 44
BsrDI GCAATG_nn'
Cuts at: 2489 2489
Size: 4577
BsrFI r'CCGG_y
Cuts at: 1533 2575 2756 2965 1533
Size: 1042 181 209 3145
Fragments arranged by size:
3145 1042 209 181
BssSI C'ACGA_G
Cuts at: 496 2848 3206 496
Size: 2352 358 1867
Fragments arranged by size:
2352 1867 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1364 1954 1364
Size: 590 3987
BstBI TT'CG_AA
Cuts at: 110 2938 110
Size: 2828 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 982 982
Size: 4577
BstYI r'GATC_y
Cuts at: 1 188 389 440 624 893 1602 2069 2092
Size: 187 201 51 184 269 709 467 23
Cuts at: 2092 2427 2673 3685 3771 3783 4129 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 709 467 449 346 335 269 246
201 187 184 86 51 23 12
BstZ17I GTA'TAC
Cuts at: 819 819
Size: 4577
BtgI C'CryG_G
Cuts at: 125 239 488 1080 125
Size: 114 249 592 3622
Fragments arranged by size:
3622 592 249 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2527 2708 2871 2527
Size: 181 163 4233
Fragments arranged by size:
4233 181 163
BtsI GCAGTG_nn'
Cuts at: 1820 1820
Size: 4577
DraI TTT'AAA
Cuts at: 1576 1576
Size: 4577
DraIII CAC_nnn'GTG
Cuts at: 1364 1364
Size: 4577
DrdI GACnn_nn'nnGTC
Cuts at: 2283 3141 2283
Size: 858 3719
EaeI y'GGCC_r
Cuts at: 128 364 712 984 1452 2162 2336 2727 2754
Size: 236 348 272 468 710 174 391 27
Cuts at: 2754 4099 128
Size: 1345 606
Fragments arranged by size:
1345 710 606 468 391 348 272 236
174 27
EagI C'GGCC_G
Cuts at: 364 2162 364
Size: 1798 2779
EarI CTCTTCn'nnn_
Cuts at: 179 221 286 295 635 2600 2810 179
Size: 42 65 9 340 1965 210 1946
Fragments arranged by size:
1965 1946 340 210 65 42 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 262 1453 2785 3095 3241 4387 262
Size: 1191 1332 310 146 1146 452
Fragments arranged by size:
1332 1191 1146 452 310 146
EcoICRI GAG'CTC
Cuts at: 1304 1589 1304
Size: 285 4292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 415 430 639 655 713 895 1110 1212
Size: 285 15 209 16 58 182 215 102
Cuts at: 1212 1278 1290 1380 1513 1565 2402 2834 3581
Size: 66 12 90 133 52 837 432 747
Cuts at: 3581 3849 130
Size: 268 858
Fragments arranged by size:
858 837 747 432 285 268 215 209
182 133 102 90 66 58 52 16
15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 178 178
Size: 4577
EcoO109I rG'GnC_Cy
Cuts at: 171 252 314 324 699 724 886 979 1549
Size: 81 62 10 375 25 162 93 570
Cuts at: 1549 1716 171
Size: 167 3032
Fragments arranged by size:
3032 570 375 167 162 93 81 62
25 10
EcoRI G'AATT_C
Cuts at: 1581 1581
Size: 4577
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1091 2596 1091
Size: 1505 3072
FspI TGC'GCA
Cuts at: 376 2358 376
Size: 1982 2595
HaeII r_GCGC'y
Cuts at: 1293 1377 1403 2011 2259 3281 4260 1293
Size: 84 26 608 248 1022 979 1610
Fragments arranged by size:
1610 1022 979 608 248 84 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 432 464 613 645 739 771 878 910 2419
Size: 32 149 32 94 32 107 32 1509
Cuts at: 2419 2449 2451 2481 432
Size: 30 2 30 2528
Fragments arranged by size:
2528 1509 149 107 94 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1616 1616
Size: 4577
KasI G'GCGC_C
Cuts at: 2007 2255 2007
Size: 248 4329
KpnI G_GTAC'C
Cuts at: 954 1597 954
Size: 643 3934
MluI A'CGCG_T
Cuts at: 4010 4010
Size: 4577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 881 1247 3248 3432 881
Size: 366 2001 184 2026
Fragments arranged by size:
2026 2001 366 184
MscI TGG'CCA
Cuts at: 130 714 986 2338 130
Size: 584 272 1352 2369
Fragments arranged by size:
2369 1352 584 272
MslI CAynn'nnrTG
Cuts at: 1421 2566 2693 1421
Size: 1145 127 3305
Fragments arranged by size:
3305 1145 127
MspA1I CmG'CkG
Cuts at: 356 513 537 678 705 861 873 1337 1494
Size: 157 24 141 27 156 12 464 157
Cuts at: 1494 1688 2002 2362 3375 3620 356
Size: 194 314 360 1013 245 1313
Fragments arranged by size:
1313 1013 464 360 314 245 194 157
157 156 141 27 24 12
NaeI GCC'GGC
Cuts at: 2758 2758
Size: 4577
NarI GG'CG_CC
Cuts at: 2008 2256 2008
Size: 248 4329
NcoI C'CATG_G
Cuts at: 125 1080 125
Size: 955 3622
NgoMIV G'CCGG_C
Cuts at: 2756 2756
Size: 4577
NsiI A_TGCA'T
Cuts at: 847 847
Size: 4577
NspI r_CATG'y
Cuts at: 1630 2130 3037 1630
Size: 500 907 3170
Fragments arranged by size:
3170 907 500
PciI A'CATG_T
Cuts at: 3033 3033
Size: 4577
PflMI CCAn_nnn'nTGG
Cuts at: 306 1536 306
Size: 1230 3347
PfoI T'CCnGG_A
Cuts at: 392 1542 392
Size: 1150 3427
PmeI GTTT'AAAC
Cuts at: 1576 1576
Size: 4577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 3716
Size: 32 4545
PpuMI rG'GwC_Cy
Cuts at: 171 252 886 171
Size: 81 634 3862
Fragments arranged by size:
3862 634 81
PspOMI G'GGCC_C
Cuts at: 313 699 313
Size: 386 4191
PstI C_TGCA'G
Cuts at: 512 1624 2309 512
Size: 1112 685 2780
Fragments arranged by size:
2780 1112 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4577
PvuII CAG'CTG
Cuts at: 678 2002 2362 678
Size: 1324 360 2893
Fragments arranged by size:
2893 1324 360
RsrII CG'GwC_CG
Cuts at: 2772 2772
Size: 4577
SacI G_AGCT'C
Cuts at: 1306 1591 1306
Size: 285 4292
SalI G'TCGA_C
Cuts at: 1614 1614
Size: 4577
SapI GCTCTTCn'nnn_
Cuts at: 286 295 2600 2810 286
Size: 9 2305 210 2053
Fragments arranged by size:
2305 2053 210 9
SbfI CC_TGCA'GG
Cuts at: 1624 1624
Size: 4577
ScaI AGT'ACT
Cuts at: 4094 4094
Size: 4577
SfcI C'TryA_G
Cuts at: 33 508 1620 2305 3298 3489 33
Size: 475 1112 685 993 191 1121
Fragments arranged by size:
1121 1112 993 685 475 191
SfoI GGC'GCC
Cuts at: 2009 2257 2009
Size: 248 4329
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
SmaI CCC'GGG
Cuts at: 319 1599 319
Size: 1280 3297
SmlI C'TyrA_G
Cuts at: 1732 2647 3139 3401 1732
Size: 915 492 262 2908
Fragments arranged by size:
2908 915 492 262
SnaBI TAC'GTA
Cuts at: 1569 1569
Size: 4577
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4577
SphI G_CATG'C
Cuts at: 1630 2130 1630
Size: 500 4077
StuI AGG'CCT
Cuts at: 1035 1035
Size: 4577
StyI C'CwwG_G
Cuts at: 125 161 380 1080 1711 2065 125
Size: 36 219 700 631 354 2637
Fragments arranged by size:
2637 700 631 354 219 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1375 2160 2293 2752 2961 1375
Size: 785 133 459 209 2991
Fragments arranged by size:
2991 785 459 209 133
TatI w'GTAC_w
Cuts at: 4092 4092
Size: 4577
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4094 4126 4094
Size: 32 4545
Tth111I GACn'n_nGTC
Cuts at: 2374 2374
Size: 4577
XbaI T'CTAG_A
Cuts at: 1608 1608
Size: 4577
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 307 2072 307
Size: 1765 2812
XmaI C'CCGG_G
Cuts at: 317 1597 317
Size: 1280 3297
XmnI GAAnn'nnTTC
Cuts at: 1291 1375 1291
Size: 84 4493
ZraI GAC'GTC
Cuts at: 1136 1136
Size: 4577
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI AvrII BaeI BbvCI BcgI
BglI BmtI BsiWI BsmI BsrGI BssHII BstEII Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SspI SwaI XhoI