Restriction map for
pF1KSDA0558
(Circular) (Six-base) MAPSORT of: pF1KSDA0558.seq Check: 5510 from: 1 to: 6572
pF1KSDA0558 6572 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1560 1560
Size: 6572
AatII G_ACGT'C
Cuts at: 238 1355 1430 238
Size: 1117 75 5380
Fragments arranged by size:
5380 1117 75
AccI GT'mk_AC
Cuts at: 368 3610 368
Size: 3242 3330
Acc65I G'GTAC_C
Cuts at: 3588 3588
Size: 6572
AclI AA'CG_TT
Cuts at: 1948 6412 1948
Size: 4464 2108
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 1066 2165 2832 4397 4829 5576 5844 547
Size: 519 1099 667 1565 432 747 268 1275
Fragments arranged by size:
1565 1275 1099 747 667 519 432 268
AflIII A'CryG_T
Cuts at: 1586 1796 2151 2357 3924 5028 6005 1586
Size: 210 355 206 1567 1104 977 2153
Fragments arranged by size:
2153 1567 1104 977 355 210 206
AgeI A'CCGG_T
Cuts at: 4960 4960
Size: 6572
AhdI GACnn_n'nnGTC
Cuts at: 1086 1086
Size: 6572
AleI CACnn'nnGTG
Cuts at: 861 2005 2584 861
Size: 1144 579 4849
Fragments arranged by size:
4849 1144 579
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 811 843 811
Size: 32 6540
AlwNI CAG_nnn'CTG
Cuts at: 525 2097 2322 2732 3243 3877 5444 525
Size: 1572 225 410 511 634 1567 1653
Fragments arranged by size:
1653 1572 1567 634 511 410 225
ApaI G_GGCC'C
Cuts at: 245 348 245
Size: 103 6469
ApaLI G'TGCA_C
Cuts at: 5342 5342
Size: 6572
ApoI r'AATT_y
Cuts at: 3576 5925 6181 3576
Size: 2349 256 3967
Fragments arranged by size:
3967 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6572
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6572
AvaI C'yCGr_G
Cuts at: 1289 2405 2972 3121 3592 1289
Size: 1116 567 149 471 4269
Fragments arranged by size:
4269 1116 567 471 149
AvrII C'CTAG_G
Cuts at: 2598 2598
Size: 6572
BamHI G'GATC_C
Cuts at: 3597 3597
Size: 6572
BanI G'GyrC_C
Cuts at: 132 151 220 227 298 1225 2007 3588 4002
Size: 19 69 7 71 927 782 1581 414
Cuts at: 4002 4250 4285 132
Size: 248 35 2419
Fragments arranged by size:
2419 1581 927 782 414 248 71 69
35 19 7
BanII G_rGCy'C
Cuts at: 245 348 800 927 2237 2456 2594 3121 3586
Size: 103 452 127 1310 219 138 527 465
Cuts at: 3586 4616 245
Size: 1030 2201
Fragments arranged by size:
2201 1310 1030 527 465 452 219 138
127 103
BbeI G_GCGC'C
Cuts at: 155 231 302 4006 4254 155
Size: 76 71 3704 248 2473
Fragments arranged by size:
3704 2473 248 76 71
BbsI GAAGACnn'nnnn_
Cuts at: 602 864 1063 2403 2496 602
Size: 262 199 1340 93 4678
Fragments arranged by size:
4678 1340 262 199 93
BbvCI CC'TCA_GC
Cuts at: 2429 2942 2429
Size: 513 6059
BciVI GTATCCnnnnn_n'
Cuts at: 4470 5231 6572 4470
Size: 761 1341 4470
Fragments arranged by size:
4470 1341 761
BclI T'GATC_A
Cuts at: 1310 4092 1310
Size: 2782 3790
BglI GCCn_nnn'nGGC
Cuts at: 3519 3519
Size: 6572
BglII A'GATC_T
Cuts at: 4087 6124 4087
Size: 2037 4535
BlpI GC'TnA_GC
Cuts at: 629 2593 3684 629
Size: 1964 1091 3517
Fragments arranged by size:
3517 1964 1091
Bme1580I G_kGCm'C
Cuts at: 245 348 1228 2940 2990 4197 4290 5346 245
Size: 103 880 1712 50 1207 93 1056 1471
Fragments arranged by size:
1712 1471 1207 1056 880 103 93 50
BmgBI CAC'GTC
Cuts at: 1710 1710
Size: 6572
BmrI ACTGGGnnnn_n'
Cuts at: 351 1612 1618 3153 3784 3865 4200 6344 351
Size: 1261 6 1535 631 81 335 2144 579
Fragments arranged by size:
2144 1535 1261 631 579 335 81 6
BmtI G_CTAG'C
Cuts at: 2680 2680
Size: 6572
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4939 4939
Size: 6572
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 838 2237 2563 2569 3204 376
Size: 462 1399 326 6 635 3744
Fragments arranged by size:
3744 1399 635 462 326 6
Bpu10I CC'TnA_GC
Cuts at: 2429 2450 2942 2429
Size: 21 492 6059
Fragments arranged by size:
6059 492 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1801 3515 3748 4627 5119 5417 1801
Size: 1714 233 879 492 298 2956
Fragments arranged by size:
2956 1714 879 492 298 233
BsaI GGTCTCn'nnnn_
Cuts at: 954 3366 3486 6276 954
Size: 2412 120 2790 1250
Fragments arranged by size:
2790 2412 1250 120
BsaAI yAC'GTr
Cuts at: 1797 2360 3564 3925 1797
Size: 563 1204 361 4444
Fragments arranged by size:
4444 1204 563 361
BsaBI GATnn'nnATC
Cuts at: 6 4086 6123 6
Size: 4080 2037 455
Fragments arranged by size:
4080 2037 455
BsaHI Gr'CG_yC
Cuts at: 152 228 235 299 351 395 614 1352 1427
Size: 76 7 64 52 44 219 738 75
Cuts at: 1427 2633 3473 4003 4251 4953 6449 152
Size: 1206 840 530 248 702 1496 275
Fragments arranged by size:
1496 1206 840 738 702 530 275 248
219 76 75 64 52 44 7
BsaWI w'CCGG_w
Cuts at: 428 1379 3383 3761 4282 4960 5234 5381 5792
Size: 951 2004 378 521 678 274 147 411
Cuts at: 5792 428
Size: 1208
Fragments arranged by size:
2004 1208 951 678 521 411 378 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 760 790 811 841 2875 2905 3482 3512 760
Size: 30 21 30 2034 30 577 30 3820
Fragments arranged by size:
3820 2034 577 30 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 3490 3490
Size: 6572
BseYI C'CCAG_C
Cuts at: 168 320 2128 3336 3468 3997 4755 5332 5828
Size: 152 1808 1208 132 529 758 577 496
Cuts at: 5828 168
Size: 912
Fragments arranged by size:
1808 1208 912 758 577 529 496 152
132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 1183 1225 1366 1594 1789 2269 2951 3309
Size: 645 42 141 228 195 480 682 358
Cuts at: 3309 3388 538
Size: 79 3722
Fragments arranged by size:
3722 682 645 480 358 228 195 141
79 42
BsiEI CG_ry'CG
Cuts at: 104 3523 4114 4160 5368 104
Size: 3419 591 46 1208 1308
Fragments arranged by size:
3419 1308 1208 591 46
BsiHKAI G_wGCw'C
Cuts at: 800 2023 2048 2237 2594 3586 4364 4554 5346
Size: 1223 25 189 357 992 778 190 792
Cuts at: 5346 800
Size: 2026
Fragments arranged by size:
2026 1223 992 792 778 357 190 189
25
BsmI GAATG_Cn'
Cuts at: 2196 2196
Size: 6572
BsmBI CGTCTCn'nnnn_
Cuts at: 3118 3319 3118
Size: 201 6371
Bsp1286I G_dGCh'C
Cuts at: 245 348 800 927 1228 2023 2048 2237 2456
Size: 103 452 127 301 795 25 189 219
Cuts at: 2456 2594 2940 2990 3121 3586 4197 4290 4364
Size: 138 346 50 131 465 611 93 74
Cuts at: 4364 4554 4616 5346 245
Size: 190 62 730 1471
Fragments arranged by size:
1471 795 730 611 465 452 346 301
219 190 189 138 131 127 103 93
74 62 50 25
BspEI T'CCGG_A
Cuts at: 5792 5792
Size: 6572
BspHI T'CATG_A
Cuts at: 1313 3146 5748 1313
Size: 1833 2602 2137
Fragments arranged by size:
2602 2137 1833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 154 858 1560 2091 2831 3622 3836 4138 4519
Size: 704 702 531 740 791 214 302 381
Cuts at: 4519 154
Size: 2207
Fragments arranged by size:
2207 791 740 704 702 531 381 302
214
BsrBI CCG'CTC
Cuts at: 50 1034 4864 4918 6402 6567 50
Size: 984 3830 54 1484 165 55
Fragments arranged by size:
3830 1484 984 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2159 4484 2159
Size: 2325 4247
BsrFI r'CCGG_y
Cuts at: 135 1243 3308 4570 4751 4960 135
Size: 1108 2065 1262 181 209 1747
Fragments arranged by size:
2065 1747 1262 1108 209 181
BsrGI T'GTAC_A
Cuts at: 747 747
Size: 6572
BssHII G'CGCG_C
Cuts at: 181 181
Size: 6572
BssSI C'ACGA_G
Cuts at: 4843 5201 4843
Size: 358 6214
BstAPI GCAn_nnn'nTGC
Cuts at: 333 2935 3296 3495 3949 333
Size: 2602 361 199 454 2956
Fragments arranged by size:
2956 2602 454 361 199
BstBI TT'CG_AA
Cuts at: 110 4933 110
Size: 4823 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 999 1548 999
Size: 549 6023
BstYI r'GATC_y
Cuts at: 1 752 1478 1898 2093 2349 3597 4064 4087
Size: 751 726 420 195 256 1248 467 23
Cuts at: 4087 4422 4668 5680 5766 5778 6124 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1248 1012 751 726 467 449 420 346
335 256 246 195 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 685 685
Size: 6572
BtgI C'CryG_G
Cuts at: 125 916 1406 1541 2493 3101 3444 125
Size: 791 490 135 952 608 343 3253
Fragments arranged by size:
3253 952 791 608 490 343 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4522 4703 4866 4522
Size: 181 163 6228
Fragments arranged by size:
6228 181 163
BtsI GCAGTG_nn'
Cuts at: 856 1032 1447 1582 2272 2630 3330 3361 3815
Size: 176 415 135 690 358 700 31 454
Cuts at: 3815 856
Size: 3613
Fragments arranged by size:
3613 700 690 454 415 358 176 135
31
DraI TTT'AAA
Cuts at: 3571 3571
Size: 6572
DraIII CAC_nnn'GTG
Cuts at: 917 1161 917
Size: 244 6328
DrdI GACnn_nn'nnGTC
Cuts at: 4278 5136 4278
Size: 858 5714
EaeI y'GGCC_r
Cuts at: 908 1217 1372 2372 3272 3306 3441 3520 4157
Size: 309 155 1000 900 34 135 79 637
Cuts at: 4157 4331 4722 4749 6094 908
Size: 174 391 27 1345 1386
Fragments arranged by size:
1386 1345 1000 900 637 391 309 174
155 135 79 34 27
EagI C'GGCC_G
Cuts at: 3520 4157 3520
Size: 637 5935
EarI CTCTTCn'nnn_
Cuts at: 523 1889 4595 4805 523
Size: 1366 2706 210 2290
Fragments arranged by size:
2706 2290 1366 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4780 5090 5236 6382 4780
Size: 310 146 1146 4970
Fragments arranged by size:
4970 1146 310 146
EcoICRI GAG'CTC
Cuts at: 798 2235 2592 3584 798
Size: 1437 357 992 3786
Fragments arranged by size:
3786 1437 992 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 547 838 1066 2165 2237 2563 2569 2832
Size: 171 291 228 1099 72 326 6 263
Cuts at: 2832 3204 4397 4829 5576 5844 376
Size: 372 1193 432 747 268 1104
Fragments arranged by size:
1193 1104 1099 747 432 372 326 291
268 263 228 171 72 6
EcoNI CCTnn'n_nnAGG
Cuts at: 439 1162 1651 439
Size: 723 489 5360
Fragments arranged by size:
5360 723 489
EcoO109I rG'GnC_Cy
Cuts at: 242 522 705 813 980 1628 2184 3449 3711
Size: 280 183 108 167 648 556 1265 262
Cuts at: 3711 242
Size: 3103
Fragments arranged by size:
3103 1265 648 556 280 262 183 167
108
EcoRI G'AATT_C
Cuts at: 3576 3576
Size: 6572
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1263 4591 1263
Size: 3328 3244
FspI TGC'GCA
Cuts at: 1177 4353 1177
Size: 3176 3396
HaeII r_GCGC'y
Cuts at: 155 231 302 884 4006 4254 5276 6255 155
Size: 76 71 582 3122 248 1022 979 472
Fragments arranged by size:
3122 1022 979 582 472 248 76 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1072 1104 4414 4444 4446 4476 1072
Size: 32 3310 30 2 30 3168
Fragments arranged by size:
3310 3168 32 30 30 2
HincII GTy'rAC
Cuts at: 369 729 2754 3611 369
Size: 360 2025 857 3330
Fragments arranged by size:
3330 2025 857 360
HpaI GTT'AAC
Cuts at: 2754 2754
Size: 6572
KasI G'GCGC_C
Cuts at: 151 227 298 4002 4250 151
Size: 76 71 3704 248 2473
Fragments arranged by size:
3704 2473 248 76 71
KpnI G_GTAC'C
Cuts at: 3592 3592
Size: 6572
MfeI C'AATT_G
Cuts at: 1368 1368
Size: 6572
MluI A'CGCG_T
Cuts at: 6005 6005
Size: 6572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1304 1885 5243 5427 1304
Size: 581 3358 184 2449
Fragments arranged by size:
3358 2449 581 184
MscI TGG'CCA
Cuts at: 1219 1374 4333 1219
Size: 155 2959 3458
Fragments arranged by size:
3458 2959 155
MslI CAynn'nnrTG
Cuts at: 130 861 1009 1421 1708 2005 2498 2522 2584
Size: 731 148 412 287 297 493 24 62
Cuts at: 2584 4561 4688 130
Size: 1977 127 2014
Fragments arranged by size:
2014 1977 731 493 412 297 287 148
127 62 24
MspA1I CmG'CkG
Cuts at: 255 282 572 1269 1437 1683 2322 2428 2541
Size: 27 290 697 168 246 639 106 113
Cuts at: 2541 2584 3083 3103 3140 3285 3340 3446 3683
Size: 43 499 20 37 145 55 106 237
Cuts at: 3683 3997 4357 5370 5615 255
Size: 314 360 1013 245 1212
Fragments arranged by size:
1212 1013 697 639 499 360 314 290
246 245 237 168 145 113 106 106
55 43 37 27 20
NaeI GCC'GGC
Cuts at: 137 1245 3310 4753 137
Size: 1108 2065 1443 1956
Fragments arranged by size:
2065 1956 1443 1108
NarI GG'CG_CC
Cuts at: 152 228 299 4003 4251 152
Size: 76 71 3704 248 2473
Fragments arranged by size:
3704 2473 248 76 71
NcoI C'CATG_G
Cuts at: 125 1541 125
Size: 1416 5156
NgoMIV G'CCGG_C
Cuts at: 135 1243 3308 4751 135
Size: 1108 2065 1443 1956
Fragments arranged by size:
2065 1956 1443 1108
NheI G'CTAG_C
Cuts at: 2676 2676
Size: 6572
NspI r_CATG'y
Cuts at: 3625 4125 5032 3625
Size: 500 907 5165
Fragments arranged by size:
5165 907 500
PasI CC'CwG_GG
Cuts at: 1663 1663
Size: 6572
PciI A'CATG_T
Cuts at: 5028 5028
Size: 6572
PflMI CCAn_nnn'nTGG
Cuts at: 917 917
Size: 6572
PfoI T'CCnGG_A
Cuts at: 1838 1862 1838
Size: 24 6548
PmeI GTTT'AAAC
Cuts at: 3571 3571
Size: 6572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 811 843 5711 5743 811
Size: 32 4868 32 1640
Fragments arranged by size:
4868 1640 32 32
PpuMI rG'GwC_Cy
Cuts at: 705 1628 2184 705
Size: 923 556 5093
Fragments arranged by size:
5093 923 556
PshAI GACnn'nnGTC
Cuts at: 1063 1063
Size: 6572
PspOMI G'GGCC_C
Cuts at: 241 344 241
Size: 103 6469
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2101 2133 2101
Size: 32 6540
PstI C_TGCA'G
Cuts at: 549 1274 1442 2102 3320 3619 4304 549
Size: 725 168 660 1218 299 685 2817
Fragments arranged by size:
2817 1218 725 685 660 299 168
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6572
PvuII CAG'CTG
Cuts at: 1269 1683 2322 2428 2584 3083 3140 3997 4357
Size: 414 639 106 156 499 57 857 360
Cuts at: 4357 1269
Size: 3484
Fragments arranged by size:
3484 857 639 499 414 360 156 106
57
RsrII CG'GwC_CG
Cuts at: 4767 4767
Size: 6572
SacI G_AGCT'C
Cuts at: 800 2237 2594 3586 800
Size: 1437 357 992 3786
Fragments arranged by size:
3786 1437 992 357
SacII CC_GC'GG
Cuts at: 3104 3447 3104
Size: 343 6229
SalI G'TCGA_C
Cuts at: 367 3609 367
Size: 3242 3330
SanDI GG'GwC_CC
Cuts at: 1628 1628
Size: 6572
SapI GCTCTTCn'nnn_
Cuts at: 4595 4805 4595
Size: 210 6362
SbfI CC_TGCA'GG
Cuts at: 2102 3619 2102
Size: 1517 5055
ScaI AGT'ACT
Cuts at: 2176 3163 6089 2176
Size: 987 2926 2659
Fragments arranged by size:
2926 2659 987
SexAI A'CCwGG_T
Cuts at: 1157 2831 1157
Size: 1674 4898
SfcI C'TryA_G
Cuts at: 33 309 545 1270 1438 2028 2055 2098 3316
Size: 276 236 725 168 590 27 43 1218
Cuts at: 3316 3615 4300 5293 5484 33
Size: 299 685 993 191 1121
Fragments arranged by size:
1218 1121 993 725 685 590 299 276
236 191 168 43 27
SfoI GGC'GCC
Cuts at: 153 229 300 4004 4252 153
Size: 76 71 3704 248 2473
Fragments arranged by size:
3704 2473 248 76 71
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6572
SmaI CCC'GGG
Cuts at: 2974 3594 2974
Size: 620 5952
SmlI C'TyrA_G
Cuts at: 1816 3530 3727 4642 5134 5396 1816
Size: 1714 197 915 492 262 2992
Fragments arranged by size:
2992 1714 915 492 262 197
SnaBI TAC'GTA
Cuts at: 3564 3564
Size: 6572
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6572
SphI G_CATG'C
Cuts at: 3625 4125 3625
Size: 500 6072
StyI C'CwwG_G
Cuts at: 125 617 1220 1541 2598 3706 4060 125
Size: 492 603 321 1057 1108 354 2637
Fragments arranged by size:
2637 1108 1057 603 492 354 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1005 1995 2950 4155 4288 4747 4956 1005
Size: 990 955 1205 133 459 209 2621
Fragments arranged by size:
2621 1205 990 955 459 209 133
TatI w'GTAC_w
Cuts at: 605 747 1364 2174 3161 6087 605
Size: 142 617 810 987 2926 1090
Fragments arranged by size:
2926 1090 987 810 617 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1437 1469 2786 2818 6089 6121 1437
Size: 32 1317 32 3271 32 1888
Fragments arranged by size:
3271 1888 1317 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1000 4369 1000
Size: 3369 3203
XbaI T'CTAG_A
Cuts at: 1521 3603 1521
Size: 2082 4490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2871 3301 4067 2871
Size: 430 766 5376
Fragments arranged by size:
5376 766 430
XmaI C'CCGG_G
Cuts at: 2972 3592 2972
Size: 620 5952
XmnI GAAnn'nnTTC
Cuts at: 436 826 1189 436
Size: 390 363 5819
Fragments arranged by size:
5819 390 363
ZraI GAC'GTC
Cuts at: 236 1353 1428 236
Size: 1117 75 5380
Fragments arranged by size:
5380 1117 75
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AlfI AscI BaeI BcgI BfrBI BsiWI
BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
NdeI NotI NruI NsiI PacI PmlI PsiI PspXI
SfiI SgrAI SrfI SspI StuI SwaI XhoI