Restriction map for
pF1KSDA0573
(Circular) (Six-base) MAPSORT of: pF1KSDA0573.seq Check: 1971 from: 1 to: 4357
pF1KSDA0573 4357 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1315 1393 1315
Size: 78 4279
Acc65I G'GTAC_C
Cuts at: 1371 1371
Size: 4357
AclI AA'CG_TT
Cuts at: 4197 4197
Size: 4357
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2180 2612 3361 3629 2180
Size: 432 749 268 2908
Fragments arranged by size:
2908 749 432 268
AflIII A'CryG_T
Cuts at: 1707 2813 3790 1707
Size: 1106 977 2274
Fragments arranged by size:
2274 1106 977
AgeI A'CCGG_T
Cuts at: 2745 2745
Size: 4357
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 114 146 114
Size: 32 4325
AlwNI CAG_nnn'CTG
Cuts at: 1660 3229 1660
Size: 1569 2788
ApaLI G'TGCA_C
Cuts at: 3127 3127
Size: 4357
ApoI r'AATT_y
Cuts at: 248 279 358 563 810 1033 1201 1359 3710
Size: 31 79 205 247 223 168 158 2351
Cuts at: 3710 3966 248
Size: 256 639
Fragments arranged by size:
2351 639 256 247 223 205 168 158
79 31
AseI AT'TA_AT
Cuts at: 20 985 1141 20
Size: 965 156 3236
Fragments arranged by size:
3236 965 156
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4357
AvaI C'yCGr_G
Cuts at: 1375 1375
Size: 4357
BamHI G'GATC_C
Cuts at: 1380 1380
Size: 4357
BanI G'GyrC_C
Cuts at: 1371 1785 2033 2068 1371
Size: 414 248 35 3660
Fragments arranged by size:
3660 414 248 35
BanII G_rGCy'C
Cuts at: 1282 1369 2399 1282
Size: 87 1030 3240
Fragments arranged by size:
3240 1030 87
BbeI G_GCGC'C
Cuts at: 1789 2037 1789
Size: 248 4109
BbsI GAAGACnn'nnnn_
Cuts at: 132 132
Size: 4357
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 660 694 660
Size: 34 4323
BciVI GTATCCnnnnn_n'
Cuts at: 2253 3016 4357 2253
Size: 763 1341 2253
Fragments arranged by size:
2253 1341 763
BclI T'GATC_A
Cuts at: 402 1875 402
Size: 1473 2884
BfrBI ATG'CAT
Cuts at: 129 129
Size: 4357
BglII A'GATC_T
Cuts at: 1870 3909 1870
Size: 2039 2318
BlpI GC'TnA_GC
Cuts at: 1467 1467
Size: 4357
Bme1580I G_kGCm'C
Cuts at: 1980 2073 3131 1980
Size: 93 1058 3206
Fragments arranged by size:
3206 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1295 1567 1648 1983 4129 1295
Size: 272 81 335 2146 1523
Fragments arranged by size:
2146 1523 335 272 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 4357
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1052 1052
Size: 4357
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 1531 2410 2904 3202 1141
Size: 390 879 494 298 2296
Fragments arranged by size:
2296 879 494 390 298
BsaI GGTCTCn'nnnn_
Cuts at: 4061 4061
Size: 4357
BsaAI yAC'GTr
Cuts at: 1347 1708 1347
Size: 361 3996
BsaBI GATnn'nnATC
Cuts at: 6 401 1869 3908 6
Size: 395 1468 2039 455
Fragments arranged by size:
2039 1468 455 395
BsaHI Gr'CG_yC
Cuts at: 1786 2034 2738 4234 1786
Size: 248 704 1496 1909
Fragments arranged by size:
1909 1496 704 248
BsaWI w'CCGG_w
Cuts at: 726 780 1544 2065 2745 3019 3166 3577 726
Size: 54 764 521 680 274 147 411 1506
Fragments arranged by size:
1506 764 680 521 411 274 147 54
BseYI C'CCAG_C
Cuts at: 1780 2538 3117 3613 1780
Size: 758 579 496 2524
Fragments arranged by size:
2524 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1036 1177 1036
Size: 141 4216
BsiEI CG_ry'CG
Cuts at: 104 1897 1943 3153 104
Size: 1793 46 1210 1308
Fragments arranged by size:
1793 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1282 1369 2147 2337 3131 1282
Size: 87 778 190 794 2508
Fragments arranged by size:
2508 794 778 190 87
BsmI GAATG_Cn'
Cuts at: 771 771
Size: 4357
Bsp1286I G_dGCh'C
Cuts at: 1282 1369 1980 2073 2147 2337 2399 3131 1282
Size: 87 611 93 74 190 62 732 2508
Fragments arranged by size:
2508 732 611 190 93 87 74 62
BspEI T'CCGG_A
Cuts at: 780 3577 780
Size: 2797 1560
BspHI T'CATG_A
Cuts at: 3533 3533
Size: 4357
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1405 1619 1921 2302 1405
Size: 214 302 381 3460
Fragments arranged by size:
3460 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2647 2701 4187 4352 50
Size: 2597 54 1486 165 55
Fragments arranged by size:
2597 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2267 2267
Size: 4357
BsrFI r'CCGG_y
Cuts at: 2353 2534 2745 2353
Size: 181 211 3965
Fragments arranged by size:
3965 211 181
BssSI C'ACGA_G
Cuts at: 2626 2986 2626
Size: 360 3997
BstAPI GCAn_nnn'nTGC
Cuts at: 1732 1732
Size: 4357
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4357
BstYI r'GATC_y
Cuts at: 1 1380 1847 1870 2205 2451 3465 3551 3563
Size: 1379 467 23 335 246 1014 86 12
Cuts at: 3563 3909 1
Size: 346 449
Fragments arranged by size:
1379 1014 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1316 1316
Size: 4357
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2305 2486 2649 2305
Size: 181 163 4013
Fragments arranged by size:
4013 181 163
BtsI GCAGTG_nn'
Cuts at: 1598 1598
Size: 4357
DraI TTT'AAA
Cuts at: 254 1354 254
Size: 1100 3257
DraIII CAC_nnn'GTG
Cuts at: 1303 1303
Size: 4357
DrdI GACnn_nn'nnGTC
Cuts at: 2061 2921 2061
Size: 860 3497
EaeI y'GGCC_r
Cuts at: 1940 2114 2505 2532 3879 1940
Size: 174 391 27 1347 2418
Fragments arranged by size:
2418 1347 391 174 27
EagI C'GGCC_G
Cuts at: 1940 1940
Size: 4357
EarI CTCTTCn'nnn_
Cuts at: 478 1052 2378 2588 478
Size: 574 1326 210 2247
Fragments arranged by size:
2247 1326 574 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2563 2875 3021 4167 2563
Size: 312 146 1146 2753
Fragments arranged by size:
2753 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1280 1367 1280
Size: 87 4270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1052 2180 2612 3361 3629 1052
Size: 1128 432 749 268 1780
Fragments arranged by size:
1780 1128 749 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1494 1494
Size: 4357
EcoRI G'AATT_C
Cuts at: 1201 1359 1201
Size: 158 4199
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 4357
FspI TGC'GCA
Cuts at: 2136 2136
Size: 4357
HaeII r_GCGC'y
Cuts at: 1789 2037 3061 4040 1789
Size: 248 1024 979 2106
Fragments arranged by size:
2106 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 303 335 394 426 2197 2227 2229 2259 303
Size: 32 59 32 1771 30 2 30 2401
Fragments arranged by size:
2401 1771 59 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1394 1394
Size: 4357
HindIII A'AGCT_T
Cuts at: 335 335
Size: 4357
KasI G'GCGC_C
Cuts at: 1785 2033 1785
Size: 248 4109
KpnI G_GTAC'C
Cuts at: 1375 1375
Size: 4357
MfeI C'AATT_G
Cuts at: 828 828
Size: 4357
MluI A'CGCG_T
Cuts at: 3790 3790
Size: 4357
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3028 3212 3028
Size: 184 4173
MscI TGG'CCA
Cuts at: 2116 2116
Size: 4357
MslI CAynn'nnrTG
Cuts at: 1112 2344 2471 1112
Size: 1232 127 2998
Fragments arranged by size:
2998 1232 127
MspA1I CmG'CkG
Cuts at: 1466 1780 2140 3155 3400 1466
Size: 314 360 1015 245 2423
Fragments arranged by size:
2423 1015 360 314 245
NaeI GCC'GGC
Cuts at: 2536 2536
Size: 4357
NarI GG'CG_CC
Cuts at: 1786 2034 1786
Size: 248 4109
NdeI CA'TA_TG
Cuts at: 1109 1109
Size: 4357
NgoMIV G'CCGG_C
Cuts at: 2534 2534
Size: 4357
NruI TCG'CGA
Cuts at: 2718 2718
Size: 4357
NsiI A_TGCA'T
Cuts at: 131 131
Size: 4357
NspI r_CATG'y
Cuts at: 1021 1408 1908 2817 1021
Size: 387 500 909 2561
Fragments arranged by size:
2561 909 500 387
PciI A'CATG_T
Cuts at: 2813 2813
Size: 4357
PfoI T'CCnGG_A
Cuts at: 1146 1146
Size: 4357
PmeI GTTT'AAAC
Cuts at: 1354 1354
Size: 4357
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3496 3528 3496
Size: 32 4325
PshAI GACnn'nnGTC
Cuts at: 296 296
Size: 4357
PstI C_TGCA'G
Cuts at: 1402 2087 1402
Size: 685 3672
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4357
PvuII CAG'CTG
Cuts at: 1780 2140 1780
Size: 360 3997
RsrII CG'GwC_CG
Cuts at: 2550 2550
Size: 4357
SacI G_AGCT'C
Cuts at: 1282 1369 1282
Size: 87 4270
SalI G'TCGA_C
Cuts at: 1392 1392
Size: 4357
SapI GCTCTTCn'nnn_
Cuts at: 2378 2588 2378
Size: 210 4147
SbfI CC_TGCA'GG
Cuts at: 1402 1402
Size: 4357
ScaI AGT'ACT
Cuts at: 3874 3874
Size: 4357
SfcI C'TryA_G
Cuts at: 33 648 1398 2083 3078 3269 33
Size: 615 750 685 995 191 1121
Fragments arranged by size:
1121 995 750 685 615 191
SfoI GGC'GCC
Cuts at: 1787 2035 1787
Size: 248 4109
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4357
SmaI CCC'GGG
Cuts at: 1377 1377
Size: 4357
SmlI C'TyrA_G
Cuts at: 1156 1510 2425 2919 3181 1156
Size: 354 915 494 262 2332
Fragments arranged by size:
2332 915 494 354 262
SnaBI TAC'GTA
Cuts at: 1347 1347
Size: 4357
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4357
SphI G_CATG'C
Cuts at: 1408 1908 1408
Size: 500 3857
SspI AAT'ATT
Cuts at: 675 959 675
Size: 284 4073
StyI C'CwwG_G
Cuts at: 1489 1843 1489
Size: 354 4003
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1314 1938 2071 2530 2741 1314
Size: 624 133 459 211 2930
Fragments arranged by size:
2930 624 459 211 133
TatI w'GTAC_w
Cuts at: 791 3872 791
Size: 3081 1276
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3874 3906 3874
Size: 32 4325
Tth111I GACn'n_nGTC
Cuts at: 2152 2152
Size: 4357
XbaI T'CTAG_A
Cuts at: 1386 1386
Size: 4357
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1850 1850
Size: 4357
XmaI C'CCGG_G
Cuts at: 1375 1375
Size: 4357
XmnI GAAnn'nnTTC
Cuts at: 359 359
Size: 4357
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NruI NsiI NspI PciI PfoI PmeI
PpiI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I
BsaXI BseRI BsiWI BsmBI BsrGI BssHII BstEII BstXI
Bsu36I BtgI ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI NcoI NheI NotI PacI PasI PflMI PmlI
PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI StuI SwaI XhoI ZraI