(Circular) (Six-base) MAPSORT of: pF1KSDA0578.seq Check: 7883 from: 1 to: 7625 pF1KSDA0578 7625 bp With 182 enzymes: SgfI * June 20, 2008 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2599 2714 3771 2599 Size: 115 1057 6453 Fragments arranged by size: 6453 1057 115 AccI GT'mk_AC Cuts at: 4613 4663 4613 Size: 50 7575 Acc65I G'GTAC_C Cuts at: 1861 4641 1861 Size: 2780 4845 AclI AA'CG_TT Cuts at: 7465 7465 Size: 7625 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1054 2102 2781 3399 3426 3599 5450 5882 6629 Size: 1048 679 618 27 173 1851 432 747 Cuts at: 6629 6897 1054 Size: 268 1782 Fragments arranged by size: 1851 1782 1048 747 679 618 432 268 173 27 AfeI AGC'GCT Cuts at: 3808 3808 Size: 7625 AflIII A'CryG_T Cuts at: 1877 2424 2993 4977 6081 7058 1877 Size: 547 569 1984 1104 977 2444 Fragments arranged by size: 2444 1984 1104 977 569 547 AgeI A'CCGG_T Cuts at: 6013 6013 Size: 7625 AhdI GACnn_n'nnGTC Cuts at: 1770 1854 1770 Size: 84 7541 AleI CACnn'nnGTG Cuts at: 1990 1990 Size: 7625 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2154 2154 Size: 7625 AlwNI CAG_nnn'CTG Cuts at: 708 2234 2818 4123 4930 6497 708 Size: 1526 584 1305 807 1567 1836 Fragments arranged by size: 1836 1567 1526 1305 807 584 ApaI G_GGCC'C Cuts at: 3350 3350 Size: 7625 ApaLI G'TGCA_C Cuts at: 6395 6395 Size: 7625 ApoI r'AATT_y Cuts at: 2285 3860 4629 6978 7234 2285 Size: 1575 769 2349 256 2676 Fragments arranged by size: 2676 2349 1575 769 256 AseI AT'TA_AT Cuts at: 20 1157 2923 3722 20 Size: 1137 1766 799 3923 Fragments arranged by size: 3923 1766 1137 799 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7625 AvaI C'yCGr_G Cuts at: 2485 4319 4645 2485 Size: 1834 326 5465 Fragments arranged by size: 5465 1834 326 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2653 2686 2653 Size: 33 7592 BamHI G'GATC_C Cuts at: 4650 4650 Size: 7625 BanI G'GyrC_C Cuts at: 229 460 1861 4641 5055 5303 5338 229 Size: 231 1401 2780 414 248 35 2516 Fragments arranged by size: 2780 2516 1401 414 248 231 35 BanII G_rGCy'C Cuts at: 662 3350 4306 4639 5669 662 Size: 2688 956 333 1030 2618 Fragments arranged by size: 2688 2618 1030 956 333 BbeI G_GCGC'C Cuts at: 233 464 5059 5307 233 Size: 231 4595 248 2551 Fragments arranged by size: 4595 2551 248 231 BbsI GAAGACnn'nnnn_ Cuts at: 905 1971 905 Size: 1066 6559 BbvCI CC'TCA_GC Cuts at: 2318 4254 2318 Size: 1936 5689 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 4027 4061 4027 Size: 34 7591 BciVI GTATCCnnnnn_n' Cuts at: 4352 5523 6284 7625 4352 Size: 1171 761 1341 4352 Fragments arranged by size: 4352 1341 1171 761 BclI T'GATC_A Cuts at: 2613 5145 2613 Size: 2532 5093 BfrBI ATG'CAT Cuts at: 2307 2883 2307 Size: 576 7049 BglII A'GATC_T Cuts at: 5140 7177 5140 Size: 2037 5588 BlpI GC'TnA_GC Cuts at: 397 419 4305 4350 4737 397 Size: 22 3886 45 387 3285 Fragments arranged by size: 3886 3285 387 45 22 Bme1580I G_kGCm'C Cuts at: 3350 5250 5343 6399 3350 Size: 1900 93 1056 4576 Fragments arranged by size: 4576 1900 1056 93 BmgBI CAC'GTC Cuts at: 684 2425 684 Size: 1741 5884 BmrI ACTGGGnnnn_n' Cuts at: 1106 3530 4837 4918 5253 7397 1106 Size: 2424 1307 81 335 2144 1334 Fragments arranged by size: 2424 2144 1334 1307 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4320 5992 4320 Size: 1672 5953 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 127 238 382 2120 2428 2647 127 Size: 111 144 1738 308 219 5105 Fragments arranged by size: 5105 1738 308 219 144 111 Bpu10I CC'TnA_GC Cuts at: 2143 2318 2853 4254 2143 Size: 175 535 1401 5514 Fragments arranged by size: 5514 1401 535 175 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 283 610 3151 4801 5680 6172 6470 283 Size: 327 2541 1650 879 492 298 1438 Fragments arranged by size: 2541 1650 1438 879 492 327 298 BsaI GGTCTCn'nnnn_ Cuts at: 7329 7329 Size: 7625 BsaAI yAC'GTr Cuts at: 2022 2994 3509 4617 4978 2022 Size: 972 515 1108 361 4669 Fragments arranged by size: 4669 1108 972 515 361 BsaBI GATnn'nnATC Cuts at: 6 1492 5139 7176 6 Size: 1486 3647 2037 455 Fragments arranged by size: 3647 2037 1486 455 BsaHI Gr'CG_yC Cuts at: 230 461 3912 5056 5304 6006 7502 230 Size: 231 3451 1144 248 702 1496 353 Fragments arranged by size: 3451 1496 1144 702 353 248 231 BsaWI w'CCGG_w Cuts at: 2209 3288 4814 5335 6013 6287 6434 6845 2209 Size: 1079 1526 521 678 274 147 411 2989 Fragments arranged by size: 2989 1526 1079 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 517 532 547 562 3283 3313 517 Size: 15 15 15 2721 30 4829 Fragments arranged by size: 4829 2721 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 687 802 1747 3203 3771 4426 687 Size: 115 945 1456 568 655 3886 Fragments arranged by size: 3886 1456 945 655 568 115 BseYI C'CCAG_C Cuts at: 194 204 1323 1360 2345 3349 4267 4555 5050 Size: 10 1119 37 985 1004 918 288 495 Cuts at: 5050 5808 6385 6881 194 Size: 758 577 496 938 Fragments arranged by size: 1119 1004 985 938 918 758 577 496 495 288 37 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 2193 2193 Size: 7625 BsiEI CG_ry'CG Cuts at: 104 387 5167 5213 6421 104 Size: 283 4780 46 1208 1308 Fragments arranged by size: 4780 1308 1208 283 46 BsiHKAI G_wGCw'C Cuts at: 329 829 4306 4639 5417 5607 6399 329 Size: 500 3477 333 778 190 792 1555 Fragments arranged by size: 3477 1555 792 778 500 333 190 BsmI GAATG_Cn' Cuts at: 1596 2360 1596 Size: 764 6861 BsmBI CGTCTCn'nnnn_ Cuts at: 1841 1841 Size: 7625 Bsp1286I G_dGCh'C Cuts at: 329 662 829 3350 4306 4639 5250 5343 5417 Size: 333 167 2521 956 333 611 93 74 Cuts at: 5417 5607 5669 6399 329 Size: 190 62 730 1555 Fragments arranged by size: 2521 1555 956 730 611 333 333 190 167 93 74 62 BspEI T'CCGG_A Cuts at: 2209 3288 6845 2209 Size: 1079 3557 2989 Fragments arranged by size: 3557 2989 1079 BspHI T'CATG_A Cuts at: 6801 6801 Size: 7625 BspMI ACCTGCnnnn'nnnn_ Cuts at: 693 2219 2599 2714 3367 3771 4675 4889 5191 Size: 1526 380 115 653 404 904 214 302 Cuts at: 5191 5572 693 Size: 381 2746 Fragments arranged by size: 2746 1526 904 653 404 381 380 302 214 115 BsrBI CCG'CTC Cuts at: 50 654 5917 5971 7455 7620 50 Size: 604 5263 54 1484 165 55 Fragments arranged by size: 5263 1484 604 165 55 54 BsrDI GCAATG_nn' Cuts at: 3465 3774 5537 3465 Size: 309 1763 5553 Fragments arranged by size: 5553 1763 309 BsrFI r'CCGG_y Cuts at: 447 868 5623 5804 6013 447 Size: 421 4755 181 209 2059 Fragments arranged by size: 4755 2059 421 209 181 BsrGI T'GTAC_A Cuts at: 3228 4448 3228 Size: 1220 6405 BssHII G'CGCG_C Cuts at: 612 612 Size: 7625 BssSI C'ACGA_G Cuts at: 322 3542 3753 5896 6254 322 Size: 3220 211 2143 358 1693 Fragments arranged by size: 3220 2143 1693 358 211 BstAPI GCAn_nnn'nTGC Cuts at: 474 708 887 2153 2535 3359 5002 474 Size: 234 179 1266 382 824 1643 3097 Fragments arranged by size: 3097 1643 1266 824 382 234 179 BstBI TT'CG_AA Cuts at: 110 5986 110 Size: 5876 1749 BstEII G'GTnAC_C Cuts at: 1382 1382 Size: 7625 BstXI CCAn_nnnn'nTGG Cuts at: 537 3994 4173 4389 537 Size: 3457 179 216 3773 Fragments arranged by size: 3773 3457 216 179 BstYI r'GATC_y Cuts at: 1 988 1479 1493 2814 4650 5117 5140 5475 Size: 987 491 14 1321 1836 467 23 335 Cuts at: 5475 5721 6733 6819 6831 7177 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1836 1321 1012 987 491 467 449 346 335 246 86 23 14 12 BtgI C'CryG_G Cuts at: 125 315 617 713 830 876 1497 1662 4382 Size: 190 302 96 117 46 621 165 2720 Cuts at: 4382 125 Size: 3368 Fragments arranged by size: 3368 2720 621 302 190 165 117 96 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3678 4038 4286 5575 5756 5919 3678 Size: 360 248 1289 181 163 5384 Fragments arranged by size: 5384 1289 360 248 181 163 BtsI GCAGTG_nn' Cuts at: 1044 1895 4121 4566 4868 1044 Size: 851 2226 445 302 3801 Fragments arranged by size: 3801 2226 851 445 302 ClaI AT'CG_AT Cuts at: 2048 2048 Size: 7625 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1253 1288 3477 3512 1253 Size: 35 2189 35 5366 Fragments arranged by size: 5366 2189 35 35 DraI TTT'AAA Cuts at: 1062 1515 2564 3275 4624 1062 Size: 453 1049 711 1349 4063 Fragments arranged by size: 4063 1349 1049 711 453 DraIII CAC_nnn'GTG Cuts at: 474 2535 3072 474 Size: 2061 537 5027 Fragments arranged by size: 5027 2061 537 DrdI GACnn_nn'nnGTC Cuts at: 688 4110 5331 6189 688 Size: 3422 1221 858 2124 Fragments arranged by size: 3422 2124 1221 858 EaeI y'GGCC_r Cuts at: 384 437 1743 2052 3557 3793 5210 5384 5775 Size: 53 1306 309 1505 236 1417 174 391 Cuts at: 5775 5802 7147 384 Size: 27 1345 862 Fragments arranged by size: 1505 1417 1345 1306 862 391 309 236 174 53 27 EagI C'GGCC_G Cuts at: 384 5210 384 Size: 4826 2799 EarI CTCTTCn'nnn_ Cuts at: 514 4188 5648 5858 514 Size: 3674 1460 210 2281 Fragments arranged by size: 3674 2281 1460 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 214 472 2392 5833 6143 6289 7435 214 Size: 258 1920 3441 310 146 1146 404 Fragments arranged by size: 3441 1920 1146 404 310 258 146 EcoICRI GAG'CTC Cuts at: 4304 4637 4304 Size: 333 7292 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 127 238 382 1054 2102 2120 2428 2647 2781 Size: 111 144 672 1048 18 308 219 134 Cuts at: 2781 3399 3426 3599 5450 5882 6629 6897 127 Size: 618 27 173 1851 432 747 268 855 Fragments arranged by size: 1851 1048 855 747 672 618 432 308 268 219 173 144 134 111 27 18 EcoNI CCTnn'n_nnAGG Cuts at: 645 645 Size: 7625 EcoO109I rG'GnC_Cy Cuts at: 705 1172 2231 2482 3346 4764 705 Size: 467 1059 251 864 1418 3566 Fragments arranged by size: 3566 1418 1059 864 467 251 EcoRI G'AATT_C Cuts at: 3860 4629 3860 Size: 769 6856 EcoRV GAT'ATC Cuts at: 2067 3080 2067 Size: 1013 6612 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1734 1990 3192 5644 1734 Size: 256 1202 2452 3715 Fragments arranged by size: 3715 2452 1202 256 FspI TGC'GCA Cuts at: 478 5406 478 Size: 4928 2697 FspAI rTGC'GCAy Cuts at: 478 478 Size: 7625 HaeII r_GCGC'y Cuts at: 139 233 314 464 625 3810 3840 5059 5307 Size: 94 81 150 161 3185 30 1219 248 Cuts at: 5307 6329 7308 139 Size: 1022 979 456 Fragments arranged by size: 3185 1219 1022 979 456 248 161 150 94 81 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 532 564 1756 1788 1840 1872 2427 2459 3283 Size: 32 1192 32 52 32 555 32 824 Cuts at: 3283 3315 4459 4491 5467 5497 5499 5529 532 Size: 32 1144 32 976 30 2 30 2628 Fragments arranged by size: 2628 1192 1144 976 824 555 52 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 453 693 1875 2570 4115 4664 453 Size: 240 1182 695 1545 549 3414 Fragments arranged by size: 3414 1545 1182 695 549 240 HpaI GTT'AAC Cuts at: 453 2570 453 Size: 2117 5508 KasI G'GCGC_C Cuts at: 229 460 5055 5303 229 Size: 231 4595 248 2551 Fragments arranged by size: 4595 2551 248 231 KpnI G_GTAC'C Cuts at: 1865 4645 1865 Size: 2780 4845 MluI A'CGCG_T Cuts at: 7058 7058 Size: 7625 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1329 2714 4598 6296 6480 1329 Size: 1385 1884 1698 184 2474 Fragments arranged by size: 2474 1884 1698 1385 184 MscI TGG'CCA Cuts at: 1745 2054 3559 3795 5386 1745 Size: 309 1505 236 1591 3984 Fragments arranged by size: 3984 1591 1505 309 236 MslI CAynn'nnrTG Cuts at: 568 1515 1990 2278 2365 2878 4126 4246 4387 Size: 947 475 288 87 513 1248 120 141 Cuts at: 4387 5614 5741 568 Size: 1227 127 2452 Fragments arranged by size: 2452 1248 1227 947 513 475 288 141 127 120 87 MspA1I CmG'CkG Cuts at: 212 317 578 619 722 758 878 3419 3791 Size: 105 261 41 103 36 120 2541 372 Cuts at: 3791 4411 4736 5050 5410 6423 6668 212 Size: 620 325 314 360 1013 245 1169 Fragments arranged by size: 2541 1169 1013 620 372 360 325 314 261 245 120 105 103 41 36 NaeI GCC'GGC Cuts at: 5806 5806 Size: 7625 NarI GG'CG_CC Cuts at: 230 461 5056 5304 230 Size: 231 4595 248 2551 Fragments arranged by size: 4595 2551 248 231 NcoI C'CATG_G Cuts at: 125 1497 1662 125 Size: 1372 165 6088 Fragments arranged by size: 6088 1372 165 NdeI CA'TA_TG Cuts at: 2355 2355 Size: 7625 NgoMIV G'CCGG_C Cuts at: 5804 5804 Size: 7625 NotI GC'GGCC_GC Cuts at: 384 384 Size: 7625 NruI TCG'CGA Cuts at: 864 864 Size: 7625 NsiI A_TGCA'T Cuts at: 2309 2885 2309 Size: 576 7049 NspI r_CATG'y Cuts at: 2172 2277 3235 4678 5178 6085 2172 Size: 105 958 1443 500 907 3712 Fragments arranged by size: 3712 1443 958 907 500 105 PasI CC'CwG_GG Cuts at: 3466 3466 Size: 7625 PciI A'CATG_T Cuts at: 6081 6081 Size: 7625 PflMI CCAn_nnn'nTGG Cuts at: 1503 1503 Size: 7625 PfoI T'CCnGG_A Cuts at: 359 1760 4364 359 Size: 1401 2604 3620 Fragments arranged by size: 3620 2604 1401 PmeI GTTT'AAAC Cuts at: 4624 4624 Size: 7625 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6764 6796 6764 Size: 32 7593 PpuMI rG'GwC_Cy Cuts at: 705 2231 2482 705 Size: 1526 251 5848 Fragments arranged by size: 5848 1526 251 PshAI GACnn'nnGTC Cuts at: 4493 4493 Size: 7625 PspOMI G'GGCC_C Cuts at: 3346 3346 Size: 7625 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3629 3661 3629 Size: 32 7593 PstI C_TGCA'G Cuts at: 395 892 2711 3819 4672 5357 395 Size: 497 1819 1108 853 685 2663 Fragments arranged by size: 2663 1819 1108 853 685 497 PvuI CG_AT'CG Cuts at: 104 104 Size: 7625 PvuII CAG'CTG Cuts at: 3419 3791 5050 5410 3419 Size: 372 1259 360 5634 Fragments arranged by size: 5634 1259 372 360 RsrII CG'GwC_CG Cuts at: 5820 5820 Size: 7625 SacI G_AGCT'C Cuts at: 4306 4639 4306 Size: 333 7292 SacII CC_GC'GG Cuts at: 318 620 879 318 Size: 302 259 7064 Fragments arranged by size: 7064 302 259 SalI G'TCGA_C Cuts at: 4662 4662 Size: 7625 SapI GCTCTTCn'nnn_ Cuts at: 514 5648 5858 514 Size: 5134 210 2281 Fragments arranged by size: 5134 2281 210 SbfI CC_TGCA'GG Cuts at: 3819 4672 3819 Size: 853 6772 ScaI AGT'ACT Cuts at: 2094 7142 2094 Size: 5048 2577 SexAI A'CCwGG_T Cuts at: 518 518 Size: 7625 SfcI C'TryA_G Cuts at: 33 391 888 2707 3426 3815 4668 5353 6346 Size: 358 497 1819 719 389 853 685 993 Cuts at: 6346 6537 33 Size: 191 1121 Fragments arranged by size: 1819 1121 993 853 719 685 497 389 358 191 SfoI GGC'GCC Cuts at: 231 462 5057 5305 231 Size: 231 4595 248 2551 Fragments arranged by size: 4595 2551 248 231 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7625 SmaI CCC'GGG Cuts at: 4647 4647 Size: 7625 SmlI C'TyrA_G Cuts at: 298 625 3166 4780 5695 6187 6449 298 Size: 327 2541 1614 915 492 262 1474 Fragments arranged by size: 2541 1614 1474 915 492 327 262 SnaBI TAC'GTA Cuts at: 4617 4617 Size: 7625 SpeI A'CTAG_T Cuts at: 67 1185 67 Size: 1118 6507 SphI G_CATG'C Cuts at: 4678 5178 4678 Size: 500 7125 StyI C'CwwG_G Cuts at: 125 942 1497 1662 4759 5113 125 Size: 817 555 165 3097 354 2637 Fragments arranged by size: 3097 2637 817 555 354 165 SwaI ATTT'AAAT Cuts at: 1515 3275 1515 Size: 1760 5865 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 525 1712 2171 3545 3613 5208 5341 5800 6009 Size: 1187 459 1374 68 1595 133 459 209 Cuts at: 6009 525 Size: 2141 Fragments arranged by size: 2141 1595 1374 1187 459 459 209 133 68 TatI w'GTAC_w Cuts at: 2092 3228 3538 4001 4448 4454 7140 2092 Size: 1136 310 463 447 6 2686 2577 Fragments arranged by size: 2686 2577 1136 463 447 310 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 517 549 7142 7174 517 Size: 32 6593 32 968 Fragments arranged by size: 6593 968 32 32 Tth111I GACn'n_nGTC Cuts at: 3875 5422 3875 Size: 1547 6078 XbaI T'CTAG_A Cuts at: 2380 2445 4656 2380 Size: 65 2211 5349 Fragments arranged by size: 5349 2211 65 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2353 3658 3780 4152 5120 2353 Size: 1305 122 372 968 4858 Fragments arranged by size: 4858 1305 968 372 122 XmaI C'CCGG_G Cuts at: 4645 4645 Size: 7625 XmnI GAAnn'nnTTC Cuts at: 3245 3245 Size: 7625 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AflII AloI AscI AvrII BglI BmtI BsiWI BstZ17I Bsu36I FseI HindIII MfeI NheI PacI PmlI PsiI PspXI SanDI SfiI SgrAI SrfI SspI StuI XhoI ZraI