Restriction map for pF1KSDA0578
 (Circular) (Six-base) MAPSORT of: pF1KSDA0578.seq  Check: 7883  from: 1  to: 7625



pF1KSDA0578  7625 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:33  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2599    2714    3771    2599 
   Size:        115    1057    6453

  Fragments arranged by size: 

                6453    1057     115
 
AccI GT'mk_AC

Cuts at:   4613    4663    4613 
   Size:         50    7575
 
Acc65I G'GTAC_C

Cuts at:   1861    4641    1861 
   Size:       2780    4845
 
AclI AA'CG_TT

Cuts at:   7465    7465 
   Size:       7625
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1054    2102    2781    3399    3426    3599    5450    5882    6629 
   Size:       1048     679     618      27     173    1851     432     747

Cuts at:   6629    6897    1054 
   Size:        268    1782

  Fragments arranged by size: 

                1851    1782    1048     747     679     618     432     268
                 173      27
 
AfeI AGC'GCT

Cuts at:   3808    3808 
   Size:       7625
 
AflIII A'CryG_T

Cuts at:   1877    2424    2993    4977    6081    7058    1877 
   Size:        547     569    1984    1104     977    2444

  Fragments arranged by size: 

                2444    1984    1104     977     569     547
 
AgeI A'CCGG_T

Cuts at:   6013    6013 
   Size:       7625
 
AhdI GACnn_n'nnGTC

Cuts at:   1770    1854    1770 
   Size:         84    7541
 
AleI CACnn'nnGTG

Cuts at:   1990    1990 
   Size:       7625
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2154    2154 
   Size:       7625
 
AlwNI CAG_nnn'CTG

Cuts at:    708    2234    2818    4123    4930    6497     708 
   Size:       1526     584    1305     807    1567    1836

  Fragments arranged by size: 

                1836    1567    1526    1305     807     584
 
ApaI G_GGCC'C

Cuts at:   3350    3350 
   Size:       7625
 
ApaLI G'TGCA_C

Cuts at:   6395    6395 
   Size:       7625
 
ApoI r'AATT_y

Cuts at:   2285    3860    4629    6978    7234    2285 
   Size:       1575     769    2349     256    2676

  Fragments arranged by size: 

                2676    2349    1575     769     256
 
AseI AT'TA_AT

Cuts at:     20    1157    2923    3722      20 
   Size:       1137    1766     799    3923

  Fragments arranged by size: 

                3923    1766    1137     799
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7625
 
AvaI C'yCGr_G

Cuts at:   2485    4319    4645    2485 
   Size:       1834     326    5465

  Fragments arranged by size: 

                5465    1834     326
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2653    2686    2653 
   Size:         33    7592
 
BamHI G'GATC_C

Cuts at:   4650    4650 
   Size:       7625
 
BanI G'GyrC_C

Cuts at:    229     460    1861    4641    5055    5303    5338     229 
   Size:        231    1401    2780     414     248      35    2516

  Fragments arranged by size: 

                2780    2516    1401     414     248     231      35
 
BanII G_rGCy'C

Cuts at:    662    3350    4306    4639    5669     662 
   Size:       2688     956     333    1030    2618

  Fragments arranged by size: 

                2688    2618    1030     956     333
 
BbeI G_GCGC'C

Cuts at:    233     464    5059    5307     233 
   Size:        231    4595     248    2551

  Fragments arranged by size: 

                4595    2551     248     231
 
BbsI GAAGACnn'nnnn_

Cuts at:    905    1971     905 
   Size:       1066    6559
 
BbvCI CC'TCA_GC

Cuts at:   2318    4254    2318 
   Size:       1936    5689
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   4027    4061    4027 
   Size:         34    7591
 
BciVI GTATCCnnnnn_n'

Cuts at:   4352    5523    6284    7625    4352 
   Size:       1171     761    1341    4352

  Fragments arranged by size: 

                4352    1341    1171     761
 
BclI T'GATC_A

Cuts at:   2613    5145    2613 
   Size:       2532    5093
 
BfrBI ATG'CAT

Cuts at:   2307    2883    2307 
   Size:        576    7049
 
BglII A'GATC_T

Cuts at:   5140    7177    5140 
   Size:       2037    5588
 
BlpI GC'TnA_GC

Cuts at:    397     419    4305    4350    4737     397 
   Size:         22    3886      45     387    3285

  Fragments arranged by size: 

                3886    3285     387      45      22
 
Bme1580I G_kGCm'C

Cuts at:   3350    5250    5343    6399    3350 
   Size:       1900      93    1056    4576

  Fragments arranged by size: 

                4576    1900    1056      93
 
BmgBI CAC'GTC

Cuts at:    684    2425     684 
   Size:       1741    5884
 
BmrI ACTGGGnnnn_n'

Cuts at:   1106    3530    4837    4918    5253    7397    1106 
   Size:       2424    1307      81     335    2144    1334

  Fragments arranged by size: 

                2424    2144    1334    1307     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4320    5992    4320 
   Size:       1672    5953
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    127     238     382    2120    2428    2647     127 
   Size:        111     144    1738     308     219    5105

  Fragments arranged by size: 

                5105    1738     308     219     144     111
 
Bpu10I CC'TnA_GC

Cuts at:   2143    2318    2853    4254    2143 
   Size:        175     535    1401    5514

  Fragments arranged by size: 

                5514    1401     535     175
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    283     610    3151    4801    5680    6172    6470     283 
   Size:        327    2541    1650     879     492     298    1438

  Fragments arranged by size: 

                2541    1650    1438     879     492     327     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   7329    7329 
   Size:       7625
 
BsaAI yAC'GTr

Cuts at:   2022    2994    3509    4617    4978    2022 
   Size:        972     515    1108     361    4669

  Fragments arranged by size: 

                4669    1108     972     515     361
 
BsaBI GATnn'nnATC

Cuts at:      6    1492    5139    7176       6 
   Size:       1486    3647    2037     455

  Fragments arranged by size: 

                3647    2037    1486     455
 
BsaHI Gr'CG_yC

Cuts at:    230     461    3912    5056    5304    6006    7502     230 
   Size:        231    3451    1144     248     702    1496     353

  Fragments arranged by size: 

                3451    1496    1144     702     353     248     231
 
BsaWI w'CCGG_w

Cuts at:   2209    3288    4814    5335    6013    6287    6434    6845    2209 
   Size:       1079    1526     521     678     274     147     411    2989

  Fragments arranged by size: 

                2989    1526    1079     678     521     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    517     532     547     562    3283    3313     517 
   Size:         15      15      15    2721      30    4829

  Fragments arranged by size: 

                4829    2721      30      15      15      15
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    687     802    1747    3203    3771    4426     687 
   Size:        115     945    1456     568     655    3886

  Fragments arranged by size: 

                3886    1456     945     655     568     115
 
BseYI C'CCAG_C

Cuts at:    194     204    1323    1360    2345    3349    4267    4555    5050 
   Size:         10    1119      37     985    1004     918     288     495

Cuts at:   5050    5808    6385    6881     194 
   Size:        758     577     496     938

  Fragments arranged by size: 

                1119    1004     985     938     918     758     577     496
                 495     288      37      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   2193    2193 
   Size:       7625
 
BsiEI CG_ry'CG

Cuts at:    104     387    5167    5213    6421     104 
   Size:        283    4780      46    1208    1308

  Fragments arranged by size: 

                4780    1308    1208     283      46
 
BsiHKAI G_wGCw'C

Cuts at:    329     829    4306    4639    5417    5607    6399     329 
   Size:        500    3477     333     778     190     792    1555

  Fragments arranged by size: 

                3477    1555     792     778     500     333     190
 
BsmI GAATG_Cn'

Cuts at:   1596    2360    1596 
   Size:        764    6861
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1841    1841 
   Size:       7625
 
Bsp1286I G_dGCh'C

Cuts at:    329     662     829    3350    4306    4639    5250    5343    5417 
   Size:        333     167    2521     956     333     611      93      74

Cuts at:   5417    5607    5669    6399     329 
   Size:        190      62     730    1555

  Fragments arranged by size: 

                2521    1555     956     730     611     333     333     190
                 167      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2209    3288    6845    2209 
   Size:       1079    3557    2989

  Fragments arranged by size: 

                3557    2989    1079
 
BspHI T'CATG_A

Cuts at:   6801    6801 
   Size:       7625
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    693    2219    2599    2714    3367    3771    4675    4889    5191 
   Size:       1526     380     115     653     404     904     214     302

Cuts at:   5191    5572     693 
   Size:        381    2746

  Fragments arranged by size: 

                2746    1526     904     653     404     381     380     302
                 214     115
 
BsrBI CCG'CTC

Cuts at:     50     654    5917    5971    7455    7620      50 
   Size:        604    5263      54    1484     165      55

  Fragments arranged by size: 

                5263    1484     604     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   3465    3774    5537    3465 
   Size:        309    1763    5553

  Fragments arranged by size: 

                5553    1763     309
 
BsrFI r'CCGG_y

Cuts at:    447     868    5623    5804    6013     447 
   Size:        421    4755     181     209    2059

  Fragments arranged by size: 

                4755    2059     421     209     181
 
BsrGI T'GTAC_A

Cuts at:   3228    4448    3228 
   Size:       1220    6405
 
BssHII G'CGCG_C

Cuts at:    612     612 
   Size:       7625
 
BssSI C'ACGA_G

Cuts at:    322    3542    3753    5896    6254     322 
   Size:       3220     211    2143     358    1693

  Fragments arranged by size: 

                3220    2143    1693     358     211
 
BstAPI GCAn_nnn'nTGC

Cuts at:    474     708     887    2153    2535    3359    5002     474 
   Size:        234     179    1266     382     824    1643    3097

  Fragments arranged by size: 

                3097    1643    1266     824     382     234     179
 
BstBI TT'CG_AA

Cuts at:    110    5986     110 
   Size:       5876    1749
 
BstEII G'GTnAC_C

Cuts at:   1382    1382 
   Size:       7625
 
BstXI CCAn_nnnn'nTGG

Cuts at:    537    3994    4173    4389     537 
   Size:       3457     179     216    3773

  Fragments arranged by size: 

                3773    3457     216     179
 
BstYI r'GATC_y

Cuts at:      1     988    1479    1493    2814    4650    5117    5140    5475 
   Size:        987     491      14    1321    1836     467      23     335

Cuts at:   5475    5721    6733    6819    6831    7177       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1836    1321    1012     987     491     467     449     346
                 335     246      86      23      14      12
 
BtgI C'CryG_G

Cuts at:    125     315     617     713     830     876    1497    1662    4382 
   Size:        190     302      96     117      46     621     165    2720

Cuts at:   4382     125 
   Size:       3368

  Fragments arranged by size: 

                3368    2720     621     302     190     165     117      96
                  46
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3678    4038    4286    5575    5756    5919    3678 
   Size:        360     248    1289     181     163    5384

  Fragments arranged by size: 

                5384    1289     360     248     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1044    1895    4121    4566    4868    1044 
   Size:        851    2226     445     302    3801

  Fragments arranged by size: 

                3801    2226     851     445     302
 
ClaI AT'CG_AT

Cuts at:   2048    2048 
   Size:       7625
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1253    1288    3477    3512    1253 
   Size:         35    2189      35    5366

  Fragments arranged by size: 

                5366    2189      35      35
 
DraI TTT'AAA

Cuts at:   1062    1515    2564    3275    4624    1062 
   Size:        453    1049     711    1349    4063

  Fragments arranged by size: 

                4063    1349    1049     711     453
 
DraIII CAC_nnn'GTG

Cuts at:    474    2535    3072     474 
   Size:       2061     537    5027

  Fragments arranged by size: 

                5027    2061     537
 
DrdI GACnn_nn'nnGTC

Cuts at:    688    4110    5331    6189     688 
   Size:       3422    1221     858    2124

  Fragments arranged by size: 

                3422    2124    1221     858
 
EaeI y'GGCC_r

Cuts at:    384     437    1743    2052    3557    3793    5210    5384    5775 
   Size:         53    1306     309    1505     236    1417     174     391

Cuts at:   5775    5802    7147     384 
   Size:         27    1345     862

  Fragments arranged by size: 

                1505    1417    1345    1306     862     391     309     236
                 174      53      27
 
EagI C'GGCC_G

Cuts at:    384    5210     384 
   Size:       4826    2799
 
EarI CTCTTCn'nnn_

Cuts at:    514    4188    5648    5858     514 
   Size:       3674    1460     210    2281

  Fragments arranged by size: 

                3674    2281    1460     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    214     472    2392    5833    6143    6289    7435     214 
   Size:        258    1920    3441     310     146    1146     404

  Fragments arranged by size: 

                3441    1920    1146     404     310     258     146
 
EcoICRI GAG'CTC

Cuts at:   4304    4637    4304 
   Size:        333    7292
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    127     238     382    1054    2102    2120    2428    2647    2781 
   Size:        111     144     672    1048      18     308     219     134

Cuts at:   2781    3399    3426    3599    5450    5882    6629    6897     127 
   Size:        618      27     173    1851     432     747     268     855

  Fragments arranged by size: 

                1851    1048     855     747     672     618     432     308
                 268     219     173     144     134     111      27      18
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    645     645 
   Size:       7625
 
EcoO109I rG'GnC_Cy

Cuts at:    705    1172    2231    2482    3346    4764     705 
   Size:        467    1059     251     864    1418    3566

  Fragments arranged by size: 

                3566    1418    1059     864     467     251
 
EcoRI G'AATT_C

Cuts at:   3860    4629    3860 
   Size:        769    6856
 
EcoRV GAT'ATC

Cuts at:   2067    3080    2067 
   Size:       1013    6612
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1734    1990    3192    5644    1734 
   Size:        256    1202    2452    3715

  Fragments arranged by size: 

                3715    2452    1202     256
 
FspI TGC'GCA

Cuts at:    478    5406     478 
   Size:       4928    2697
 
FspAI rTGC'GCAy

Cuts at:    478     478 
   Size:       7625
 
HaeII r_GCGC'y

Cuts at:    139     233     314     464     625    3810    3840    5059    5307 
   Size:         94      81     150     161    3185      30    1219     248

Cuts at:   5307    6329    7308     139 
   Size:       1022     979     456

  Fragments arranged by size: 

                3185    1219    1022     979     456     248     161     150
                  94      81      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    532     564    1756    1788    1840    1872    2427    2459    3283 
   Size:         32    1192      32      52      32     555      32     824

Cuts at:   3283    3315    4459    4491    5467    5497    5499    5529     532 
   Size:         32    1144      32     976      30       2      30    2628

  Fragments arranged by size: 

                2628    1192    1144     976     824     555      52      32
                  32      32      32      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    453     693    1875    2570    4115    4664     453 
   Size:        240    1182     695    1545     549    3414

  Fragments arranged by size: 

                3414    1545    1182     695     549     240
 
HpaI GTT'AAC

Cuts at:    453    2570     453 
   Size:       2117    5508
 
KasI G'GCGC_C

Cuts at:    229     460    5055    5303     229 
   Size:        231    4595     248    2551

  Fragments arranged by size: 

                4595    2551     248     231
 
KpnI G_GTAC'C

Cuts at:   1865    4645    1865 
   Size:       2780    4845
 
MluI A'CGCG_T

Cuts at:   7058    7058 
   Size:       7625
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1329    2714    4598    6296    6480    1329 
   Size:       1385    1884    1698     184    2474

  Fragments arranged by size: 

                2474    1884    1698    1385     184
 
MscI TGG'CCA

Cuts at:   1745    2054    3559    3795    5386    1745 
   Size:        309    1505     236    1591    3984

  Fragments arranged by size: 

                3984    1591    1505     309     236
 
MslI CAynn'nnrTG

Cuts at:    568    1515    1990    2278    2365    2878    4126    4246    4387 
   Size:        947     475     288      87     513    1248     120     141

Cuts at:   4387    5614    5741     568 
   Size:       1227     127    2452

  Fragments arranged by size: 

                2452    1248    1227     947     513     475     288     141
                 127     120      87
 
MspA1I CmG'CkG

Cuts at:    212     317     578     619     722     758     878    3419    3791 
   Size:        105     261      41     103      36     120    2541     372

Cuts at:   3791    4411    4736    5050    5410    6423    6668     212 
   Size:        620     325     314     360    1013     245    1169

  Fragments arranged by size: 

                2541    1169    1013     620     372     360     325     314
                 261     245     120     105     103      41      36
 
NaeI GCC'GGC

Cuts at:   5806    5806 
   Size:       7625
 
NarI GG'CG_CC

Cuts at:    230     461    5056    5304     230 
   Size:        231    4595     248    2551

  Fragments arranged by size: 

                4595    2551     248     231
 
NcoI C'CATG_G

Cuts at:    125    1497    1662     125 
   Size:       1372     165    6088

  Fragments arranged by size: 

                6088    1372     165
 
NdeI CA'TA_TG

Cuts at:   2355    2355 
   Size:       7625
 
NgoMIV G'CCGG_C

Cuts at:   5804    5804 
   Size:       7625
 
NotI GC'GGCC_GC

Cuts at:    384     384 
   Size:       7625
 
NruI TCG'CGA

Cuts at:    864     864 
   Size:       7625
 
NsiI A_TGCA'T

Cuts at:   2309    2885    2309 
   Size:        576    7049
 
NspI r_CATG'y

Cuts at:   2172    2277    3235    4678    5178    6085    2172 
   Size:        105     958    1443     500     907    3712

  Fragments arranged by size: 

                3712    1443     958     907     500     105
 
PasI CC'CwG_GG

Cuts at:   3466    3466 
   Size:       7625
 
PciI A'CATG_T

Cuts at:   6081    6081 
   Size:       7625
 
PflMI CCAn_nnn'nTGG

Cuts at:   1503    1503 
   Size:       7625
 
PfoI T'CCnGG_A

Cuts at:    359    1760    4364     359 
   Size:       1401    2604    3620

  Fragments arranged by size: 

                3620    2604    1401
 
PmeI GTTT'AAAC

Cuts at:   4624    4624 
   Size:       7625
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   6764    6796    6764 
   Size:         32    7593
 
PpuMI rG'GwC_Cy

Cuts at:    705    2231    2482     705 
   Size:       1526     251    5848

  Fragments arranged by size: 

                5848    1526     251
 
PshAI GACnn'nnGTC

Cuts at:   4493    4493 
   Size:       7625
 
PspOMI G'GGCC_C

Cuts at:   3346    3346 
   Size:       7625
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   3629    3661    3629 
   Size:         32    7593
 
PstI C_TGCA'G

Cuts at:    395     892    2711    3819    4672    5357     395 
   Size:        497    1819    1108     853     685    2663

  Fragments arranged by size: 

                2663    1819    1108     853     685     497
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       7625
 
PvuII CAG'CTG

Cuts at:   3419    3791    5050    5410    3419 
   Size:        372    1259     360    5634

  Fragments arranged by size: 

                5634    1259     372     360
 
RsrII CG'GwC_CG

Cuts at:   5820    5820 
   Size:       7625
 
SacI G_AGCT'C

Cuts at:   4306    4639    4306 
   Size:        333    7292
 
SacII CC_GC'GG

Cuts at:    318     620     879     318 
   Size:        302     259    7064

  Fragments arranged by size: 

                7064     302     259
 
SalI G'TCGA_C

Cuts at:   4662    4662 
   Size:       7625
 
SapI GCTCTTCn'nnn_

Cuts at:    514    5648    5858     514 
   Size:       5134     210    2281

  Fragments arranged by size: 

                5134    2281     210
 
SbfI CC_TGCA'GG

Cuts at:   3819    4672    3819 
   Size:        853    6772
 
ScaI AGT'ACT

Cuts at:   2094    7142    2094 
   Size:       5048    2577
 
SexAI A'CCwGG_T

Cuts at:    518     518 
   Size:       7625
 
SfcI C'TryA_G

Cuts at:     33     391     888    2707    3426    3815    4668    5353    6346 
   Size:        358     497    1819     719     389     853     685     993

Cuts at:   6346    6537      33 
   Size:        191    1121

  Fragments arranged by size: 

                1819    1121     993     853     719     685     497     389
                 358     191
 
SfoI GGC'GCC

Cuts at:    231     462    5057    5305     231 
   Size:        231    4595     248    2551

  Fragments arranged by size: 

                4595    2551     248     231
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7625
 
SmaI CCC'GGG

Cuts at:   4647    4647 
   Size:       7625
 
SmlI C'TyrA_G

Cuts at:    298     625    3166    4780    5695    6187    6449     298 
   Size:        327    2541    1614     915     492     262    1474

  Fragments arranged by size: 

                2541    1614    1474     915     492     327     262
 
SnaBI TAC'GTA

Cuts at:   4617    4617 
   Size:       7625
 
SpeI A'CTAG_T

Cuts at:     67    1185      67 
   Size:       1118    6507
 
SphI G_CATG'C

Cuts at:   4678    5178    4678 
   Size:        500    7125
 
StyI C'CwwG_G

Cuts at:    125     942    1497    1662    4759    5113     125 
   Size:        817     555     165    3097     354    2637

  Fragments arranged by size: 

                3097    2637     817     555     354     165
 
SwaI ATTT'AAAT

Cuts at:   1515    3275    1515 
   Size:       1760    5865
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    525    1712    2171    3545    3613    5208    5341    5800    6009 
   Size:       1187     459    1374      68    1595     133     459     209

Cuts at:   6009     525 
   Size:       2141

  Fragments arranged by size: 

                2141    1595    1374    1187     459     459     209     133
                  68
 
TatI w'GTAC_w

Cuts at:   2092    3228    3538    4001    4448    4454    7140    2092 
   Size:       1136     310     463     447       6    2686    2577

  Fragments arranged by size: 

                2686    2577    1136     463     447     310       6
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    517     549    7142    7174     517 
   Size:         32    6593      32     968

  Fragments arranged by size: 

                6593     968      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3875    5422    3875 
   Size:       1547    6078
 
XbaI T'CTAG_A

Cuts at:   2380    2445    4656    2380 
   Size:         65    2211    5349

  Fragments arranged by size: 

                5349    2211      65
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2353    3658    3780    4152    5120    2353 
   Size:       1305     122     372     968    4858

  Fragments arranged by size: 

                4858    1305     968     372     122
 
XmaI C'CCGG_G

Cuts at:   4645    4645 
   Size:       7625
 
XmnI GAAnn'nnTTC

Cuts at:   3245    3245 
   Size:       7625
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI
     NruI     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI   PspOMI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfcI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AloI     AscI    AvrII     BglI     BmtI    BsiWI
  BstZ17I   Bsu36I     FseI  HindIII     MfeI     NheI     PacI     PmlI
     PsiI    PspXI    SanDI     SfiI    SgrAI     SrfI     SspI     StuI
     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com