Restriction map for
pF1KSDA0588
(Circular) (Six-base) MAPSORT of: pF1KSDA0588.seq Check: 155 from: 1 to: 5933
pF1KSDA0588 5933 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2866 2866
Size: 5933
AccI GT'mk_AC
Cuts at: 2810 2971 2810
Size: 161 5772
Acc65I G'GTAC_C
Cuts at: 2949 2949
Size: 5933
AclI AA'CG_TT
Cuts at: 5773 5773
Size: 5933
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2291 3758 4190 4937 5205 2291
Size: 1467 432 747 268 3019
Fragments arranged by size:
3019 1467 747 432 268
AflIII A'CryG_T
Cuts at: 1637 1760 3285 4389 5366 1637
Size: 123 1525 1104 977 2204
Fragments arranged by size:
2204 1525 1104 977 123
AgeI A'CCGG_T
Cuts at: 4321 4321
Size: 5933
AleI CACnn'nnGTG
Cuts at: 1846 1846
Size: 5933
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 922 922
Size: 5933
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 114 146 114
Size: 32 5901
AlwNI CAG_nnn'CTG
Cuts at: 568 3238 4805 568
Size: 2670 1567 1696
Fragments arranged by size:
2670 1696 1567
ApaLI G'TGCA_C
Cuts at: 4703 4703
Size: 5933
ApoI r'AATT_y
Cuts at: 2937 5286 5542 2937
Size: 2349 256 3328
Fragments arranged by size:
3328 2349 256
AseI AT'TA_AT
Cuts at: 20 1219 20
Size: 1199 4734
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5933
AvaI C'yCGr_G
Cuts at: 293 859 1804 2158 2953 293
Size: 566 945 354 795 3273
Fragments arranged by size:
3273 945 795 566 354
BamHI G'GATC_C
Cuts at: 600 2958 600
Size: 2358 3575
BanI G'GyrC_C
Cuts at: 796 1739 2599 2632 2731 2737 2949 3363 3611
Size: 943 860 33 99 6 212 414 248
Cuts at: 3611 3646 796
Size: 35 3083
Fragments arranged by size:
3083 943 860 414 248 212 99 35
33 6
BanII G_rGCy'C
Cuts at: 293 302 410 641 1681 1877 2019 2495 2716
Size: 9 108 231 1040 196 142 476 221
Cuts at: 2716 2947 3977 293
Size: 231 1030 2249
Fragments arranged by size:
2249 1040 1030 476 231 231 221 196
142 108 9
BbeI G_GCGC'C
Cuts at: 3367 3615 3367
Size: 248 5685
BbsI GAAGACnn'nnnn_
Cuts at: 1470 2226 1470
Size: 756 5177
BbvCI CC'TCA_GC
Cuts at: 1750 1750
Size: 5933
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1610 1644 1610
Size: 34 5899
BciVI GTATCCnnnnn_n'
Cuts at: 3831 4592 5933 3831
Size: 761 1341 3831
Fragments arranged by size:
3831 1341 761
BclI T'GATC_A
Cuts at: 1127 3453 1127
Size: 2326 3607
BglII A'GATC_T
Cuts at: 3448 5485 3448
Size: 2037 3896
BlpI GC'TnA_GC
Cuts at: 640 854 1675 3045 640
Size: 214 821 1370 3528
Fragments arranged by size:
3528 1370 821 214
Bme1580I G_kGCm'C
Cuts at: 901 1742 2792 3558 3651 4707 901
Size: 841 1050 766 93 1056 2127
Fragments arranged by size:
2127 1056 1050 841 766 93
BmgBI CAC'GTC
Cuts at: 1143 1143
Size: 5933
BmrI ACTGGGnnnn_n'
Cuts at: 1667 2763 3145 3226 3561 5705 1667
Size: 1096 382 81 335 2144 1895
Fragments arranged by size:
2144 1895 1096 382 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 267 1552 2053 4300 267
Size: 1285 501 2247 1900
Fragments arranged by size:
2247 1900 1285 501
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 262 307 2150 255
Size: 7 45 1843 4038
Fragments arranged by size:
4038 1843 45 7
Bpu10I CC'TnA_GC
Cuts at: 1750 2266 1750
Size: 516 5417
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1936 2023 3109 3988 4480 4778 1936
Size: 87 1086 879 492 298 3091
Fragments arranged by size:
3091 1086 879 492 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 193 2229 2586 5637 193
Size: 2036 357 3051 489
Fragments arranged by size:
3051 2036 489 357
BsaAI yAC'GTr
Cuts at: 1638 2787 2925 3286 1638
Size: 1149 138 361 4285
Fragments arranged by size:
4285 1149 361 138
BsaBI GATnn'nnATC
Cuts at: 6 3447 5484 6
Size: 3441 2037 455
Fragments arranged by size:
3441 2037 455
BsaHI Gr'CG_yC
Cuts at: 2335 2686 3364 3612 4314 5810 2335
Size: 351 678 248 702 1496 2458
Fragments arranged by size:
2458 1496 702 678 351 248
BsaWI w'CCGG_w
Cuts at: 202 231 603 879 968 3122 3643 4321 4595
Size: 29 372 276 89 2154 521 678 274
Cuts at: 4595 4742 5153 202
Size: 147 411 982
Fragments arranged by size:
2154 982 678 521 411 372 276 274
147 89 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 470 500 1900 1930 2905 2935 470
Size: 30 1400 30 975 30 3468
Fragments arranged by size:
3468 1400 975 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 418 1069 1541 418
Size: 651 472 4810
Fragments arranged by size:
4810 651 472
BseYI C'CCAG_C
Cuts at: 171 2438 2871 3358 4116 4693 5189 171
Size: 2267 433 487 758 577 496 915
Fragments arranged by size:
2267 915 758 577 496 487 433
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 1972 2383 127
Size: 1845 411 3677
Fragments arranged by size:
3677 1845 411
BsiEI CG_ry'CG
Cuts at: 104 3475 3521 4729 104
Size: 3371 46 1208 1308
Fragments arranged by size:
3371 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 302 410 641 1681 2716 2947 3725 3915 4707
Size: 108 231 1040 1035 231 778 190 792
Cuts at: 4707 302
Size: 1528
Fragments arranged by size:
1528 1040 1035 792 778 231 231 190
108
BsmBI CGTCTCn'nnnn_
Cuts at: 1545 2024 1545
Size: 479 5454
Bsp1286I G_dGCh'C
Cuts at: 293 302 410 641 901 1681 1742 1877 2019
Size: 9 108 231 260 780 61 135 142
Cuts at: 2019 2495 2716 2792 2947 3558 3651 3725 3915
Size: 476 221 76 155 611 93 74 190
Cuts at: 3915 3977 4707 293
Size: 62 730 1519
Fragments arranged by size:
1519 780 730 611 476 260 231 221
190 155 142 135 108 93 76 74
62 61 9
BspEI T'CCGG_A
Cuts at: 231 603 879 5153 231
Size: 372 276 4274 1011
Fragments arranged by size:
4274 1011 372 276
BspHI T'CATG_A
Cuts at: 5109 5109
Size: 5933
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2866 2983 3197 3499 3880 2866
Size: 117 214 302 381 4919
Fragments arranged by size:
4919 381 302 214 117
BsrBI CCG'CTC
Cuts at: 50 1865 4225 4279 5763 5928 50
Size: 1815 2360 54 1484 165 55
Fragments arranged by size:
2360 1815 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1094 2832 2889 3845 1094
Size: 1738 57 956 3182
Fragments arranged by size:
3182 1738 956 57
BsrFI r'CCGG_y
Cuts at: 1473 2331 2629 2734 3931 4112 4321 1473
Size: 858 298 105 1197 181 209 3085
Fragments arranged by size:
3085 1197 858 298 209 181 105
BssHII G'CGCG_C
Cuts at: 802 802
Size: 5933
BssSI C'ACGA_G
Cuts at: 1051 2389 4204 4562 1051
Size: 1338 1815 358 2422
Fragments arranged by size:
2422 1815 1338 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2787 3310 2787
Size: 523 5410
BstBI TT'CG_AA
Cuts at: 110 4294 110
Size: 4184 1749
BstEII G'GTnAC_C
Cuts at: 1887 1887
Size: 5933
BstXI CCAn_nnnn'nTGG
Cuts at: 279 1848 1899 2682 279
Size: 1569 51 783 3530
Fragments arranged by size:
3530 1569 783 51
BstYI r'GATC_y
Cuts at: 1 600 1808 2958 3425 3448 3783 4029 5041
Size: 599 1208 1150 467 23 335 246 1012
Cuts at: 5041 5127 5139 5485 1
Size: 86 12 346 449
Fragments arranged by size:
1208 1150 1012 599 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1484 1484
Size: 5933
BtgI C'CryG_G
Cuts at: 1424 1829 2070 2108 2114 2219 2405 2741 1424
Size: 405 241 38 6 105 186 336 4616
Fragments arranged by size:
4616 405 336 241 186 105 38 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2636 3883 4064 4227 2636
Size: 1247 181 163 4342
Fragments arranged by size:
4342 1247 181 163
BtsI GCAGTG_nn'
Cuts at: 3176 3176
Size: 5933
DraI TTT'AAA
Cuts at: 1232 1302 2932 1232
Size: 70 1630 4233
Fragments arranged by size:
4233 1630 70
DraIII CAC_nnn'GTG
Cuts at: 2346 2346
Size: 5933
DrdI GACnn_nn'nnGTC
Cuts at: 940 2461 3639 4497 940
Size: 1521 1178 858 2376
Fragments arranged by size:
2376 1521 1178 858
EaeI y'GGCC_r
Cuts at: 1920 2057 2073 2111 2117 2216 3518 3692 4083
Size: 137 16 38 6 99 1302 174 391
Cuts at: 4083 4110 5455 1920
Size: 27 1345 2398
Fragments arranged by size:
2398 1345 1302 391 174 137 99 38
27 16 6
EagI C'GGCC_G
Cuts at: 3518 3518
Size: 5933
EarI CTCTTCn'nnn_
Cuts at: 230 1565 1978 2413 3956 4166 230
Size: 1335 413 435 1543 210 1997
Fragments arranged by size:
1997 1543 1335 435 413 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2079 2158 2679 4141 4451 4597 5743 2079
Size: 79 521 1462 310 146 1146 2269
Fragments arranged by size:
2269 1462 1146 521 310 146 79
EcoICRI GAG'CTC
Cuts at: 300 408 639 1679 2714 2945 300
Size: 108 231 1040 1035 231 3288
Fragments arranged by size:
3288 1040 1035 231 231 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 262 307 2150 2291 3758 4190 4937 5205
Size: 7 45 1843 141 1467 432 747 268
Cuts at: 5205 255
Size: 983
Fragments arranged by size:
1843 1467 983 747 432 268 141 45
7
EcoNI CCTnn'n_nnAGG
Cuts at: 935 2299 935
Size: 1364 4569
EcoO109I rG'GnC_Cy
Cuts at: 276 2868 3072 276
Size: 2592 204 3137
Fragments arranged by size:
3137 2592 204
EcoRI G'AATT_C
Cuts at: 2937 2937
Size: 5933
EcoRV GAT'ATC
Cuts at: 609 609
Size: 5933
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3952 3952
Size: 5933
FspI TGC'GCA
Cuts at: 790 2005 3714 790
Size: 1215 1709 3009
Fragments arranged by size:
3009 1709 1215
FspAI rTGC'GCAy
Cuts at: 790 2005 790
Size: 1215 4718
HaeII r_GCGC'y
Cuts at: 2266 2280 2332 2756 3367 3615 4637 5616 2266
Size: 14 52 424 611 248 1022 979 2583
Fragments arranged by size:
2583 1022 979 611 424 248 52 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 305 337 1106 1138 1256 1288 1900 1932 2698
Size: 32 769 32 118 32 612 32 766
Cuts at: 2698 2730 3775 3805 3807 3837 305
Size: 32 1045 30 2 30 2401
Fragments arranged by size:
2401 1045 769 766 612 118 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 918 2972 918
Size: 2054 3879
KasI G'GCGC_C
Cuts at: 3363 3611 3363
Size: 248 5685
KpnI G_GTAC'C
Cuts at: 2953 2953
Size: 5933
MluI A'CGCG_T
Cuts at: 5366 5366
Size: 5933
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 794 1025 1676 4604 4788 794
Size: 231 651 2928 184 1939
Fragments arranged by size:
2928 1939 651 231 184
MscI TGG'CCA
Cuts at: 3694 3694
Size: 5933
MslI CAynn'nnrTG
Cuts at: 451 571 811 1387 1846 2662 3922 4049 451
Size: 120 240 576 459 816 1260 127 2335
Fragments arranged by size:
2335 1260 816 576 459 240 127 120
MspA1I CmG'CkG
Cuts at: 368 906 2221 2606 2848 3044 3358 3718 4731
Size: 538 1315 385 242 196 314 360 1013
Cuts at: 4731 4976 368
Size: 245 1325
Fragments arranged by size:
1325 1315 1013 538 385 360 314 245
242 196
NaeI GCC'GGC
Cuts at: 2333 2736 4114 2333
Size: 403 1378 4152
Fragments arranged by size:
4152 1378 403
NarI GG'CG_CC
Cuts at: 3364 3612 3364
Size: 248 5685
NcoI C'CATG_G
Cuts at: 2741 2741
Size: 5933
NgoMIV G'CCGG_C
Cuts at: 2331 2734 4112 2331
Size: 403 1378 4152
Fragments arranged by size:
4152 1378 403
NspI r_CATG'y
Cuts at: 2460 2986 3486 4393 2460
Size: 526 500 907 4000
Fragments arranged by size:
4000 907 526 500
PasI CC'CwG_GG
Cuts at: 2721 2721
Size: 5933
PciI A'CATG_T
Cuts at: 4389 4389
Size: 5933
PflMI CCAn_nnn'nTGG
Cuts at: 2139 2878 2139
Size: 739 5194
PfoI T'CCnGG_A
Cuts at: 2063 2063
Size: 5933
PmeI GTTT'AAAC
Cuts at: 2932 2932
Size: 5933
PmlI CAC'GTG
Cuts at: 2787 2787
Size: 5933
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 562 594 5072 5104 562
Size: 32 4478 32 1391
Fragments arranged by size:
4478 1391 32 32
PpuMI rG'GwC_Cy
Cuts at: 276 276
Size: 5933
PspXI vC'TCGA_Gb
Cuts at: 2158 2158
Size: 5933
PstI C_TGCA'G
Cuts at: 629 2303 2378 2783 2980 3665 629
Size: 1674 75 405 197 685 2897
Fragments arranged by size:
2897 1674 685 405 197 75
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5933
PvuII CAG'CTG
Cuts at: 906 2848 3358 3718 906
Size: 1942 510 360 3121
Fragments arranged by size:
3121 1942 510 360
RsrII CG'GwC_CG
Cuts at: 4128 4128
Size: 5933
SacI G_AGCT'C
Cuts at: 302 410 641 1681 2716 2947 302
Size: 108 231 1040 1035 231 3288
Fragments arranged by size:
3288 1040 1035 231 231 108
SacII CC_GC'GG
Cuts at: 2222 2222
Size: 5933
SalI G'TCGA_C
Cuts at: 2970 2970
Size: 5933
SapI GCTCTTCn'nnn_
Cuts at: 230 3956 4166 230
Size: 3726 210 1997
Fragments arranged by size:
3726 1997 210
SbfI CC_TGCA'GG
Cuts at: 2980 2980
Size: 5933
ScaI AGT'ACT
Cuts at: 5450 5450
Size: 5933
SexAI A'CCwGG_T
Cuts at: 752 1883 2201 752
Size: 1131 318 4484
Fragments arranged by size:
4484 1131 318
SfcI C'TryA_G
Cuts at: 33 625 1329 2299 2374 2779 2976 3661 4654
Size: 592 704 970 75 405 197 685 993
Cuts at: 4654 4845 33
Size: 191 1121
Fragments arranged by size:
1121 993 970 704 685 592 405 197
191 75
SfoI GGC'GCC
Cuts at: 3365 3613 3365
Size: 248 5685
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5933
SgrAI Cr'CCGG_yG
Cuts at: 2331 2331
Size: 5933
SmaI CCC'GGG
Cuts at: 295 2955 295
Size: 2660 3273
SmlI C'TyrA_G
Cuts at: 1951 2038 2158 3088 4003 4495 4757 1951
Size: 87 120 930 915 492 262 3127
Fragments arranged by size:
3127 930 915 492 262 120 87
SnaBI TAC'GTA
Cuts at: 2925 2925
Size: 5933
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5933
SphI G_CATG'C
Cuts at: 2986 3486 2986
Size: 500 5433
StuI AGG'CCT
Cuts at: 1489 1489
Size: 5933
StyI C'CwwG_G
Cuts at: 893 1284 1614 1892 2741 3067 3421 893
Size: 391 330 278 849 326 354 3405
Fragments arranged by size:
3405 849 391 354 330 326 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 226 3516 3649 4108 4317 226
Size: 3290 133 459 209 1842
Fragments arranged by size:
3290 1842 459 209 133
TatI w'GTAC_w
Cuts at: 5448 5448
Size: 5933
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2380 2412 5450 5482 2380
Size: 32 3038 32 2831
Fragments arranged by size:
3038 2831 32 32
Tth111I GACn'n_nGTC
Cuts at: 1348 3730 1348
Size: 2382 3551
XbaI T'CTAG_A
Cuts at: 441 2964 441
Size: 2523 3410
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3428 3428
Size: 5933
XhoI C'TCGA_G
Cuts at: 2158 2158
Size: 5933
XmaI C'CCGG_G
Cuts at: 293 2953 293
Size: 2660 3273
XmnI GAAnn'nnTTC
Cuts at: 1367 1367
Size: 5933
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI ApaI AscI AvrII BaeI
BfrBI BglI BmtI BsiWI BsmI BsrGI BstZ17I ClaI
CspCI FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PshAI PsiI PspOMI PsrI SanDI
SfiI SrfI SspI SwaI ZraI