Restriction map for
pF1KSDA0601
(Circular) (Six-base) MAPSORT of: pF1KSDA0601.seq Check: 7019 from: 1 to: 5695
pF1KSDA0601 5695 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 2626 2731 2626
Size: 105 5590
Acc65I G'GTAC_C
Cuts at: 2709 2709
Size: 5695
AclI AA'CG_TT
Cuts at: 5535 5535
Size: 5695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 1544 1633 1901 2035 3518 3950 4699 4967
Size: 894 89 268 134 1483 432 749 268
Cuts at: 4967 650
Size: 1378
Fragments arranged by size:
1483 1378 894 749 432 268 268 134
89
AflII C'TTAA_G
Cuts at: 1771 1771
Size: 5695
AflIII A'CryG_T
Cuts at: 3045 4151 5128 3045
Size: 1106 977 3612
Fragments arranged by size:
3612 1106 977
AgeI A'CCGG_T
Cuts at: 1274 4083 1274
Size: 2809 2886
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1217 1249 2519 2551 1217
Size: 32 1270 32 4361
Fragments arranged by size:
4361 1270 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2998 4567 2998
Size: 1569 4126
ApaI G_GGCC'C
Cuts at: 265 265
Size: 5695
ApaLI G'TGCA_C
Cuts at: 4465 4465
Size: 5695
ApoI r'AATT_y
Cuts at: 1473 1735 2697 5048 5304 1473
Size: 262 962 2351 256 1864
Fragments arranged by size:
2351 1864 962 262 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5695
AvaI C'yCGr_G
Cuts at: 337 2396 2713 337
Size: 2059 317 3319
Fragments arranged by size:
3319 2059 317
BamHI G'GATC_C
Cuts at: 2139 2718 2139
Size: 579 5116
BanI G'GyrC_C
Cuts at: 2488 2709 3123 3371 3406 2488
Size: 221 414 248 35 4777
Fragments arranged by size:
4777 414 248 221 35
BanII G_rGCy'C
Cuts at: 265 2198 2397 2404 2707 3737 265
Size: 1933 199 7 303 1030 2223
Fragments arranged by size:
2223 1933 1030 303 199 7
BbeI G_GCGC'C
Cuts at: 3127 3375 3127
Size: 248 5447
BbvCI CC'TCA_GC
Cuts at: 2348 2348
Size: 5695
BciVI GTATCCnnnnn_n'
Cuts at: 3591 4354 5695 3591
Size: 763 1341 3591
Fragments arranged by size:
3591 1341 763
BclI T'GATC_A
Cuts at: 3213 3213
Size: 5695
BglI GCCn_nnn'nGGC
Cuts at: 157 260 157
Size: 103 5592
BglII A'GATC_T
Cuts at: 3208 5247 3208
Size: 2039 3656
BlpI GC'TnA_GC
Cuts at: 2456 2805 2456
Size: 349 5346
Bme1580I G_kGCm'C
Cuts at: 265 2493 3318 3411 4469 265
Size: 2228 825 93 1058 1491
Fragments arranged by size:
2228 1491 1058 825 93
BmrI ACTGGGnnnn_n'
Cuts at: 1788 2905 2986 3321 5467 1788
Size: 1117 81 335 2146 2016
Fragments arranged by size:
2146 2016 1117 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4060 4060
Size: 5695
Bpu10I CC'TnA_GC
Cuts at: 2348 2348
Size: 5695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 680 2869 3748 4242 4540 578
Size: 102 2189 879 494 298 1733
Fragments arranged by size:
2189 1733 879 494 298 102
BsaI GGTCTCn'nnnn_
Cuts at: 993 5399 993
Size: 4406 1289
BsaAI yAC'GTr
Cuts at: 2685 3046 2685
Size: 361 5334
BsaBI GATnn'nnATC
Cuts at: 6 1682 3207 5246 6
Size: 1676 1525 2039 455
Fragments arranged by size:
2039 1676 1525 455
BsaHI Gr'CG_yC
Cuts at: 447 3124 3372 4076 5572 447
Size: 2677 248 704 1496 570
Fragments arranged by size:
2677 1496 704 570 248
BsaWI w'CCGG_w
Cuts at: 438 683 1274 2882 3403 4083 4357 4504 4915
Size: 245 591 1608 521 680 274 147 411
Cuts at: 4915 438
Size: 1218
Fragments arranged by size:
1608 1218 680 591 521 411 274 245
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1106 1136 1106
Size: 30 5665
BseYI C'CCAG_C
Cuts at: 264 2547 3118 3876 4455 4951 264
Size: 2283 571 758 579 496 1008
Fragments arranged by size:
2283 1008 758 579 571 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2360 2645 2360
Size: 285 5410
BsiEI CG_ry'CG
Cuts at: 104 3235 3281 4491 104
Size: 3131 46 1210 1308
Fragments arranged by size:
3131 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 2198 2568 2707 3485 3675 4469 2198
Size: 370 139 778 190 794 3424
Fragments arranged by size:
3424 794 778 370 190 139
Bsp1286I G_dGCh'C
Cuts at: 265 2198 2397 2404 2493 2568 2707 3318 3411
Size: 1933 199 7 89 75 139 611 93
Cuts at: 3411 3485 3675 3737 4469 265
Size: 74 190 62 732 1491
Fragments arranged by size:
1933 1491 732 611 199 190 139 93
89 75 74 62 7
BspEI T'CCGG_A
Cuts at: 438 4915 438
Size: 4477 1218
BspHI T'CATG_A
Cuts at: 678 4871 678
Size: 4193 1502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2743 2957 3259 3640 2743
Size: 214 302 381 4798
Fragments arranged by size:
4798 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1949 3985 4039 5525 5690 50
Size: 1899 2036 54 1486 165 55
Fragments arranged by size:
2036 1899 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3605 3605
Size: 5695
BsrFI r'CCGG_y
Cuts at: 379 458 1274 3691 3872 4083 379
Size: 79 816 2417 181 211 1991
Fragments arranged by size:
2417 1991 816 211 181 79
BssSI C'ACGA_G
Cuts at: 1839 1953 3964 4324 1839
Size: 114 2011 360 3210
Fragments arranged by size:
3210 2011 360 114
BstAPI GCAn_nnn'nTGC
Cuts at: 3070 3070
Size: 5695
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5695
BstYI r'GATC_y
Cuts at: 1 1581 2139 2718 3185 3208 3543 3789 4803
Size: 1580 558 579 467 23 335 246 1014
Cuts at: 4803 4889 4901 5247 1
Size: 86 12 346 449
Fragments arranged by size:
1580 1014 579 558 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 2627 2627
Size: 5695
Bsu36I CC'TnA_GG
Cuts at: 374 605 374
Size: 231 5464
BtgI C'CryG_G
Cuts at: 152 248 322 413 1118 152
Size: 96 74 91 705 4729
Fragments arranged by size:
4729 705 96 91 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3643 3824 3987 3643
Size: 181 163 5351
Fragments arranged by size:
5351 181 163
BtsI GCAGTG_nn'
Cuts at: 775 1898 2343 2936 775
Size: 1123 445 593 3534
Fragments arranged by size:
3534 1123 593 445
DraI TTT'AAA
Cuts at: 2692 2692
Size: 5695
DraIII CAC_nnn'GTG
Cuts at: 2653 2653
Size: 5695
DrdI GACnn_nn'nnGTC
Cuts at: 1321 3399 4259 1321
Size: 2078 860 2757
Fragments arranged by size:
2757 2078 860
EaeI y'GGCC_r
Cuts at: 236 506 1184 1350 2298 2309 3278 3452 3843
Size: 270 678 166 948 11 969 174 391
Cuts at: 3843 3870 5217 236
Size: 27 1347 714
Fragments arranged by size:
1347 969 948 714 678 391 270 174
166 27 11
EagI C'GGCC_G
Cuts at: 3278 3278
Size: 5695
EarI CTCTTCn'nnn_
Cuts at: 536 2203 2209 2518 3716 3926 536
Size: 1667 6 309 1198 210 2305
Fragments arranged by size:
2305 1667 1198 309 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 364 3901 4213 4359 5505 364
Size: 3537 312 146 1146 554
Fragments arranged by size:
3537 1146 554 312 146
EcoICRI GAG'CTC
Cuts at: 2196 2705 2196
Size: 509 5186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 1544 1633 1901 2035 3518 3950 4699 4967
Size: 894 89 268 134 1483 432 749 268
Cuts at: 4967 650
Size: 1378
Fragments arranged by size:
1483 1378 894 749 432 268 268 134
89
EcoNI CCTnn'n_nnAGG
Cuts at: 1351 1597 2104 1351
Size: 246 507 4942
Fragments arranged by size:
4942 507 246
EcoO109I rG'GnC_Cy
Cuts at: 342 370 2832 342
Size: 28 2462 3205
Fragments arranged by size:
3205 2462 28
EcoRI G'AATT_C
Cuts at: 2697 2697
Size: 5695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1474 1663 3712 1474
Size: 189 2049 3457
Fragments arranged by size:
3457 2049 189
FseI GG_CCGG'CC
Cuts at: 383 383
Size: 5695
FspI TGC'GCA
Cuts at: 3474 3474
Size: 5695
HaeII r_GCGC'y
Cuts at: 3127 3375 4399 5378 3127
Size: 248 1024 979 3444
Fragments arranged by size:
3444 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 211 243 678 710 3535 3565 3567 3597 211
Size: 32 435 32 2825 30 2 30 2309
Fragments arranged by size:
2825 2309 435 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2732 2732
Size: 5695
HindIII A'AGCT_T
Cuts at: 501 574 501
Size: 73 5622
KasI G'GCGC_C
Cuts at: 3123 3371 3123
Size: 248 5447
KpnI G_GTAC'C
Cuts at: 2713 2713
Size: 5695
MluI A'CGCG_T
Cuts at: 5128 5128
Size: 5695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 430 770 855 1637 4366 4550 430
Size: 340 85 782 2729 184 1575
Fragments arranged by size:
2729 1575 782 340 184 85
MscI TGG'CCA
Cuts at: 508 1186 1352 2311 3454 508
Size: 678 166 959 1143 2749
Fragments arranged by size:
2749 1143 959 678 166
MslI CAynn'nnrTG
Cuts at: 3682 3809 3682
Size: 127 5568
MspA1I CmG'CkG
Cuts at: 154 172 250 324 810 2804 3118 3478 4493
Size: 18 78 74 486 1994 314 360 1015
Cuts at: 4493 4738 154
Size: 245 1111
Fragments arranged by size:
1994 1111 1015 486 360 314 245 78
74 18
NaeI GCC'GGC
Cuts at: 381 460 3874 381
Size: 79 3414 2202
Fragments arranged by size:
3414 2202 79
NarI GG'CG_CC
Cuts at: 3124 3372 3124
Size: 248 5447
NcoI C'CATG_G
Cuts at: 413 1118 413
Size: 705 4990
NgoMIV G'CCGG_C
Cuts at: 379 458 3872 379
Size: 79 3414 2202
Fragments arranged by size:
3414 2202 79
NruI TCG'CGA
Cuts at: 4056 4056
Size: 5695
NspI r_CATG'y
Cuts at: 1393 2167 2682 2746 3246 4155 1393
Size: 774 515 64 500 909 2933
Fragments arranged by size:
2933 909 774 515 500 64
PasI CC'CwG_GG
Cuts at: 199 2004 2390 199
Size: 1805 386 3504
Fragments arranged by size:
3504 1805 386
PciI A'CATG_T
Cuts at: 4151 4151
Size: 5695
PflMI CCAn_nnn'nTGG
Cuts at: 1359 2085 2613 1359
Size: 726 528 4441
Fragments arranged by size:
4441 726 528
PmeI GTTT'AAAC
Cuts at: 2692 2692
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4834 4866 4834
Size: 32 5663
PsiI TTA'TAA
Cuts at: 851 1973 851
Size: 1122 4573
PspOMI G'GGCC_C
Cuts at: 261 261
Size: 5695
PstI C_TGCA'G
Cuts at: 171 782 2076 2424 2740 3425 171
Size: 611 1294 348 316 685 2441
Fragments arranged by size:
2441 1294 685 611 348 316
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5695
PvuII CAG'CTG
Cuts at: 810 3118 3478 810
Size: 2308 360 3027
Fragments arranged by size:
3027 2308 360
RsrII CG'GwC_CG
Cuts at: 3888 3888
Size: 5695
SacI G_AGCT'C
Cuts at: 2198 2707 2198
Size: 509 5186
SacII CC_GC'GG
Cuts at: 155 251 325 155
Size: 96 74 5525
Fragments arranged by size:
5525 96 74
SalI G'TCGA_C
Cuts at: 2730 2730
Size: 5695
SapI GCTCTTCn'nnn_
Cuts at: 2203 3716 3926 2203
Size: 1513 210 3972
Fragments arranged by size:
3972 1513 210
SbfI CC_TGCA'GG
Cuts at: 2740 2740
Size: 5695
ScaI AGT'ACT
Cuts at: 5212 5212
Size: 5695
SexAI A'CCwGG_T
Cuts at: 2649 2649
Size: 5695
SfcI C'TryA_G
Cuts at: 33 167 778 2072 2420 2736 3421 4416 4607
Size: 134 611 1294 348 316 685 995 191
Cuts at: 4607 33
Size: 1121
Fragments arranged by size:
1294 1121 995 685 611 348 316 191
134
SfiI GGCCn_nnn'nGGCC
Cuts at: 260 260
Size: 5695
SfoI GGC'GCC
Cuts at: 3125 3373 3125
Size: 248 5447
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5695
SmaI CCC'GGG
Cuts at: 339 2715 339
Size: 2376 3319
SmlI C'TyrA_G
Cuts at: 593 695 1771 2848 3763 4257 4519 593
Size: 102 1076 1077 915 494 262 1769
Fragments arranged by size:
1769 1077 1076 915 494 262 102
SnaBI TAC'GTA
Cuts at: 2685 2685
Size: 5695
SpeI A'CTAG_T
Cuts at: 67 2241 67
Size: 2174 3521
SphI G_CATG'C
Cuts at: 2746 3246 2746
Size: 500 5195
StuI AGG'CCT
Cuts at: 1868 1868
Size: 5695
StyI C'CwwG_G
Cuts at: 413 1118 1347 2827 3181 413
Size: 705 229 1480 354 2927
Fragments arranged by size:
2927 1480 705 354 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1054 1551 1996 3276 3409 3868 4079 1054
Size: 497 445 1280 133 459 211 2670
Fragments arranged by size:
2670 1280 497 459 445 211 133
TatI w'GTAC_w
Cuts at: 482 5210 482
Size: 4728 967
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1106 1138 1913 1945 5212 5244 1106
Size: 32 775 32 3267 32 1557
Fragments arranged by size:
3267 1557 775 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3490 3490
Size: 5695
XbaI T'CTAG_A
Cuts at: 2724 2724
Size: 5695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1059 2361 3188 1059
Size: 1302 827 3566
Fragments arranged by size:
3566 1302 827
XmaI C'CCGG_G
Cuts at: 337 2713 337
Size: 2376 3319
XmnI GAAnn'nnTTC
Cuts at: 1646 1646
Size: 5695
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PflMI PmeI PpiI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BpmI BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI ClaI
CspCI EcoRV FspAI HpaI MfeI NdeI NheI NotI
NsiI PacI PfoI PmlI PpuMI PshAI PspXI PsrI
SanDI SgrAI SrfI SspI SwaI XhoI ZraI