Restriction map for
pF1KSDA0608
(Circular) (Six-base) MAPSORT of: pF1KSDA0608.seq Check: 6421 from: 1 to: 5446
pF1KSDA0608 5446 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 980 980
Size: 5446
AccI GT'mk_AC
Cuts at: 2482 2482
Size: 5446
Acc65I G'GTAC_C
Cuts at: 2460 2460
Size: 5446
AclI AA'CG_TT
Cuts at: 5286 5286
Size: 5446
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 1159 3269 3701 4450 4718 301
Size: 858 2110 432 749 268 1029
Fragments arranged by size:
2110 1029 858 749 432 268
AflIII A'CryG_T
Cuts at: 932 2796 3902 4879 932
Size: 1864 1106 977 1499
Fragments arranged by size:
1864 1499 1106 977
AgeI A'CCGG_T
Cuts at: 3834 3834
Size: 5446
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1037 1860 1037
Size: 823 4623
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2296 2328 2296
Size: 32 5414
AlwNI CAG_nnn'CTG
Cuts at: 785 2749 4318 785
Size: 1964 1569 1913
Fragments arranged by size:
1964 1913 1569
ApaLI G'TGCA_C
Cuts at: 4216 4216
Size: 5446
ApoI r'AATT_y
Cuts at: 1263 1270 2188 2448 4799 5055 1263
Size: 7 918 260 2351 256 1654
Fragments arranged by size:
2351 1654 918 260 256 7
AscI GG'CGCG_CC
Cuts at: 845 845
Size: 5446
AseI AT'TA_AT
Cuts at: 20 1744 20
Size: 1724 3722
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5446
AvaI C'yCGr_G
Cuts at: 156 444 519 742 762 835 1037 2464 156
Size: 288 75 223 20 73 202 1427 3138
Fragments arranged by size:
3138 1427 288 223 202 75 73 20
BamHI G'GATC_C
Cuts at: 2469 2469
Size: 5446
BanI G'GyrC_C
Cuts at: 183 394 823 993 2460 2874 3122 3157 183
Size: 211 429 170 1467 414 248 35 2472
Fragments arranged by size:
2472 1467 429 414 248 211 170 35
BanII G_rGCy'C
Cuts at: 362 445 451 743 1044 2458 3488 362
Size: 83 6 292 301 1414 1030 2320
Fragments arranged by size:
2320 1414 1030 301 292 83 6
BbeI G_GCGC'C
Cuts at: 398 827 2878 3126 398
Size: 429 2051 248 2718
Fragments arranged by size:
2718 2051 429 248
BbsI GAAGACnn'nnnn_
Cuts at: 1241 1241
Size: 5446
BbvCI CC'TCA_GC
Cuts at: 1385 1385
Size: 5446
BciVI GTATCCnnnnn_n'
Cuts at: 3342 4105 5446 3342
Size: 763 1341 3342
Fragments arranged by size:
3342 1341 763
BclI T'GATC_A
Cuts at: 2964 2964
Size: 5446
BglI GCCn_nnn'nGGC
Cuts at: 306 392 440 614 862 306
Size: 86 48 174 248 4890
Fragments arranged by size:
4890 248 174 86 48
BglII A'GATC_T
Cuts at: 2959 4998 2959
Size: 2039 3407
BlpI GC'TnA_GC
Cuts at: 2556 2556
Size: 5446
Bme1580I G_kGCm'C
Cuts at: 998 3069 3162 4220 998
Size: 2071 93 1058 2224
Fragments arranged by size:
2224 2071 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 2656 2737 3072 5218 2656
Size: 81 335 2146 2884
Fragments arranged by size:
2884 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3811 3811
Size: 5446
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 649 370
Size: 279 5167
Bpu10I CC'TnA_GC
Cuts at: 1385 1385
Size: 5446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1324 1632 2620 3499 3993 4291 712
Size: 612 308 988 879 494 298 1867
Fragments arranged by size:
1867 988 879 612 494 308 298
BsaI GGTCTCn'nnnn_
Cuts at: 5150 5150
Size: 5446
BsaAI yAC'GTr
Cuts at: 2436 2797 2436
Size: 361 5085
BsaBI GATnn'nnATC
Cuts at: 6 2958 4997 6
Size: 2952 2039 455
Fragments arranged by size:
2952 2039 455
BsaHI Gr'CG_yC
Cuts at: 251 298 395 428 731 824 2875 3123 3827
Size: 47 97 33 303 93 2051 248 704
Cuts at: 3827 5323 251
Size: 1496 374
Fragments arranged by size:
2051 1496 704 374 303 248 97 93
47 33
BsaWI w'CCGG_w
Cuts at: 135 2633 3154 3834 4108 4255 4666 135
Size: 2498 521 680 274 147 411 915
Fragments arranged by size:
2498 915 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1215 1245 1215
Size: 30 5416
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 301 304 307 853 289
Size: 12 3 3 546 4882
Fragments arranged by size:
4882 546 12 3 3
BseYI C'CCAG_C
Cuts at: 438 2869 3627 4206 4702 438
Size: 2431 758 579 496 1182
Fragments arranged by size:
2431 1182 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 338 424 588 618 866 2986 3032 4242
Size: 234 86 164 30 248 2120 46 1210
Cuts at: 4242 104
Size: 1308
Fragments arranged by size:
2120 1308 1210 248 234 164 86 46
30
BsiHKAI G_wGCw'C
Cuts at: 1131 2458 3236 3426 4220 1131
Size: 1327 778 190 794 2357
Fragments arranged by size:
2357 1327 794 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 289 289
Size: 5446
Bsp1286I G_dGCh'C
Cuts at: 362 445 451 743 998 1044 1131 2458 3069
Size: 83 6 292 255 46 87 1327 611
Cuts at: 3069 3162 3236 3426 3488 4220 362
Size: 93 74 190 62 732 1588
Fragments arranged by size:
1588 1327 732 611 292 255 190 93
87 83 74 62 46 6
BspEI T'CCGG_A
Cuts at: 135 4666 135
Size: 4531 915
BspHI T'CATG_A
Cuts at: 1433 1809 4622 1433
Size: 376 2813 2257
Fragments arranged by size:
2813 2257 376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 980 2494 2708 3010 3391 980
Size: 1514 214 302 381 3035
Fragments arranged by size:
3035 1514 381 302 214
BsrBI CCG'CTC
Cuts at: 50 375 3736 3790 5276 5441 50
Size: 325 3361 54 1486 165 55
Fragments arranged by size:
3361 1486 325 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1481 1886 3356 1481
Size: 405 1470 3571
Fragments arranged by size:
3571 1470 405
BsrFI r'CCGG_y
Cuts at: 391 580 990 3442 3623 3834 391
Size: 189 410 2452 181 211 2003
Fragments arranged by size:
2452 2003 410 211 189 181
BssHII G'CGCG_C
Cuts at: 485 574 576 845 485
Size: 89 2 269 5086
Fragments arranged by size:
5086 269 89 2
BssSI C'ACGA_G
Cuts at: 3715 4075 3715
Size: 360 5086
BstAPI GCAn_nnn'nTGC
Cuts at: 1560 2821 1560
Size: 1261 4185
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5446
BstXI CCAn_nnnn'nTGG
Cuts at: 1338 1338
Size: 5446
BstYI r'GATC_y
Cuts at: 1 2108 2469 2936 2959 3294 3540 4554 4640
Size: 2107 361 467 23 335 246 1014 86
Cuts at: 4640 4652 4998 1
Size: 12 346 449
Fragments arranged by size:
2107 1014 467 449 361 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 200 230 459 490 725 948 1007 1575
Size: 75 30 229 31 235 223 59 568
Cuts at: 1575 125
Size: 3996
Fragments arranged by size:
3996 568 235 229 223 75 59 31
30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3394 3575 3738 3394
Size: 181 163 5102
Fragments arranged by size:
5102 181 163
BtsI GCAGTG_nn'
Cuts at: 1289 2687 1289
Size: 1398 4048
DraI TTT'AAA
Cuts at: 2443 2443
Size: 5446
DrdI GACnn_nn'nnGTC
Cuts at: 3150 4010 3150
Size: 860 4586
EaeI y'GGCC_r
Cuts at: 335 384 480 585 615 722 863 3029 3203
Size: 49 96 105 30 107 141 2166 174
Cuts at: 3203 3594 3621 4968 335
Size: 391 27 1347 813
Fragments arranged by size:
2166 1347 813 391 174 141 107 105
96 49 30 27
EagI C'GGCC_G
Cuts at: 335 585 615 863 3029 335
Size: 250 30 248 2166 2752
Fragments arranged by size:
2752 2166 250 248 30
EarI CTCTTCn'nnn_
Cuts at: 551 1527 1910 3467 3677 551
Size: 976 383 1557 210 2320
Fragments arranged by size:
2320 1557 976 383 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 260 3652 3964 4110 5256 260
Size: 3392 312 146 1146 450
Fragments arranged by size:
3392 1146 450 312 146
EcoICRI GAG'CTC
Cuts at: 2456 2456
Size: 5446
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 370 649 1159 3269 3701 4450 4718 301
Size: 69 279 510 2110 432 749 268 1029
Fragments arranged by size:
2110 1029 749 510 432 279 268 69
EcoO109I rG'GnC_Cy
Cuts at: 634 696 832 1018 1226 2583 634
Size: 62 136 186 208 1357 3497
Fragments arranged by size:
3497 1357 208 186 136 62
EcoRI G'AATT_C
Cuts at: 2448 2448
Size: 5446
EcoRV GAT'ATC
Cuts at: 2295 2295
Size: 5446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 3463 2059
Size: 1404 4042
FspI TGC'GCA
Cuts at: 3225 3225
Size: 5446
HaeII r_GCGC'y
Cuts at: 260 398 827 962 2878 3126 4150 5129 260
Size: 138 429 135 1916 248 1024 979 577
Fragments arranged by size:
1916 1024 979 577 429 248 138 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 288 320 667 699 936 968 1300 1332 2198
Size: 32 347 32 237 32 332 32 866
Cuts at: 2198 2230 2284 2316 3286 3316 3318 3348 288
Size: 32 54 32 970 30 2 30 2386
Fragments arranged by size:
2386 970 866 347 332 237 54 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2483 2483
Size: 5446
HindIII A'AGCT_T
Cuts at: 1346 1346
Size: 5446
KasI G'GCGC_C
Cuts at: 394 823 2874 3122 394
Size: 429 2051 248 2718
Fragments arranged by size:
2718 2051 429 248
KpnI G_GTAC'C
Cuts at: 2464 2464
Size: 5446
MfeI C'AATT_G
Cuts at: 1506 1506
Size: 5446
MluI A'CGCG_T
Cuts at: 4879 4879
Size: 5446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1117 1733 4117 4301 1117
Size: 616 2384 184 2262
Fragments arranged by size:
2384 2262 616 184
MscI TGG'CCA
Cuts at: 386 3205 386
Size: 2819 2627
MslI CAynn'nnrTG
Cuts at: 1842 2335 3433 3560 1842
Size: 493 1098 127 3728
Fragments arranged by size:
3728 1098 493 127
MspA1I CmG'CkG
Cuts at: 348 382 461 492 702 727 785 813 950
Size: 34 79 31 210 25 58 28 137
Cuts at: 950 1009 2555 2869 3229 4244 4489 348
Size: 59 1546 314 360 1015 245 1305
Fragments arranged by size:
1546 1305 1015 360 314 245 210 137
79 59 58 34 31 28 25
NaeI GCC'GGC
Cuts at: 393 582 3625 393
Size: 189 3043 2214
Fragments arranged by size:
3043 2214 189
NarI GG'CG_CC
Cuts at: 395 824 2875 3123 395
Size: 429 2051 248 2718
Fragments arranged by size:
2718 2051 429 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 5446
NgoMIV G'CCGG_C
Cuts at: 391 580 3623 391
Size: 189 3043 2214
Fragments arranged by size:
3043 2214 189
NruI TCG'CGA
Cuts at: 548 3807 548
Size: 3259 2187
NspI r_CATG'y
Cuts at: 1847 2497 2997 3906 1847
Size: 650 500 909 3387
Fragments arranged by size:
3387 909 650 500
PacI TTA_AT'TAA
Cuts at: 1748 1748
Size: 5446
PciI A'CATG_T
Cuts at: 3902 3902
Size: 5446
PflMI CCAn_nnn'nTGG
Cuts at: 2357 2357
Size: 5446
PmeI GTTT'AAAC
Cuts at: 2443 2443
Size: 5446
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4585 4617 4585
Size: 32 5414
PpuMI rG'GwC_Cy
Cuts at: 696 832 1018 1226 696
Size: 136 186 208 4916
Fragments arranged by size:
4916 208 186 136
PshAI GACnn'nnGTC
Cuts at: 2365 2365
Size: 5446
PstI C_TGCA'G
Cuts at: 790 2246 2491 3176 790
Size: 1456 245 685 3060
Fragments arranged by size:
3060 1456 685 245
PvuI CG_AT'CG
Cuts at: 104 424 104
Size: 320 5126
PvuII CAG'CTG
Cuts at: 348 785 2869 3229 348
Size: 437 2084 360 2565
Fragments arranged by size:
2565 2084 437 360
RsrII CG'GwC_CG
Cuts at: 3639 3639
Size: 5446
SacI G_AGCT'C
Cuts at: 2458 2458
Size: 5446
SacII CC_GC'GG
Cuts at: 462 493 728 951 1010 462
Size: 31 235 223 59 4898
Fragments arranged by size:
4898 235 223 59 31
SalI G'TCGA_C
Cuts at: 2481 2481
Size: 5446
SanDI GG'GwC_CC
Cuts at: 832 832
Size: 5446
SapI GCTCTTCn'nnn_
Cuts at: 3467 3677 3467
Size: 210 5236
SbfI CC_TGCA'GG
Cuts at: 2491 2491
Size: 5446
ScaI AGT'ACT
Cuts at: 1898 4963 1898
Size: 3065 2381
SfcI C'TryA_G
Cuts at: 33 786 1598 1848 2242 2487 3172 4167 4358
Size: 753 812 250 394 245 685 995 191
Cuts at: 4358 33
Size: 1121
Fragments arranged by size:
1121 995 812 753 685 394 250 245
191
SfoI GGC'GCC
Cuts at: 396 825 2876 3124 396
Size: 429 2051 248 2718
Fragments arranged by size:
2718 2051 429 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5446
SgrAI Cr'CCGG_yG
Cuts at: 580 990 580
Size: 410 5036
SmaI CCC'GGG
Cuts at: 1039 2466 1039
Size: 1427 4019
SmlI C'TyrA_G
Cuts at: 691 1303 1647 2599 3514 4008 4270 691
Size: 612 344 952 915 494 262 1867
Fragments arranged by size:
1867 952 915 612 494 344 262
SnaBI TAC'GTA
Cuts at: 2436 2436
Size: 5446
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5446
SphI G_CATG'C
Cuts at: 2497 2997 2497
Size: 500 4946
SrfI GCCC'GGGC
Cuts at: 1039 1039
Size: 5446
SspI AAT'ATT
Cuts at: 1464 2008 1464
Size: 544 4902
StuI AGG'CCT
Cuts at: 1854 1854
Size: 5446
StyI C'CwwG_G
Cuts at: 125 1872 2578 2932 125
Size: 1747 706 354 2639
Fragments arranged by size:
2639 1747 706 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3027 3160 3619 3830 3027
Size: 133 459 211 4643
Fragments arranged by size:
4643 459 211 133
TatI w'GTAC_w
Cuts at: 1525 1896 4961 1525
Size: 371 3065 2010
Fragments arranged by size:
3065 2010 371
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4963 4995 4963
Size: 32 5414
Tth111I GACn'n_nGTC
Cuts at: 3241 3241
Size: 5446
XbaI T'CTAG_A
Cuts at: 2475 2475
Size: 5446
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1129 2939 1129
Size: 1810 3636
XmaI C'CCGG_G
Cuts at: 1037 2464 1037
Size: 1427 4019
XmnI GAAnn'nnTTC
Cuts at: 1672 2234 1672
Size: 562 4884
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PacI PciI PflMI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsgI BsiWI BsmI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HpaI NdeI NheI NotI NsiI PasI PfoI
PmlI PsiI PspOMI PspXI PsrI SexAI SfiI SwaI
XhoI ZraI