Restriction map for
pF1KSDA0613
(Circular) (Six-base) MAPSORT of: pF1KSDA0613.seq Check: 9905 from: 1 to: 5320
pF1KSDA0613 5320 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1104 1266 1527 2029 2212 1104
Size: 162 261 502 183 4212
Fragments arranged by size:
4212 502 261 183 162
AccI GT'mk_AC
Cuts at: 842 860 1346 2356 842
Size: 18 486 1010 3806
Fragments arranged by size:
3806 1010 486 18
Acc65I G'GTAC_C
Cuts at: 2334 2334
Size: 5320
AclI AA'CG_TT
Cuts at: 1910 5160 1910
Size: 3250 2070
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1235 1862 3143 3575 4324 4592 1235
Size: 627 1281 432 749 268 1963
Fragments arranged by size:
1963 1281 749 627 432 268
AflIII A'CryG_T
Cuts at: 2670 3776 4753 2670
Size: 1106 977 3237
Fragments arranged by size:
3237 1106 977
AgeI A'CCGG_T
Cuts at: 3708 3708
Size: 5320
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1902 1958 1902
Size: 56 5264
AlwNI CAG_nnn'CTG
Cuts at: 1144 1183 1258 1360 1665 2623 4192 1144
Size: 39 75 102 305 958 1569 2272
Fragments arranged by size:
2272 1569 958 305 102 75 39
ApaI G_GGCC'C
Cuts at: 155 161 657 1288 1567 1807 155
Size: 6 496 631 279 240 3668
Fragments arranged by size:
3668 631 496 279 240 6
ApaLI G'TGCA_C
Cuts at: 1047 4090 1047
Size: 3043 2277
ApoI r'AATT_y
Cuts at: 979 2322 4673 4929 979
Size: 1343 2351 256 1370
Fragments arranged by size:
2351 1370 1343 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5320
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5320
AvaI C'yCGr_G
Cuts at: 154 656 690 792 1310 1629 1670 2338 154
Size: 502 34 102 518 319 41 668 3136
Fragments arranged by size:
3136 668 518 502 319 102 41 34
BamHI G'GATC_C
Cuts at: 2343 2343
Size: 5320
BanI G'GyrC_C
Cuts at: 480 517 526 1707 1835 2334 2748 2996 3031
Size: 37 9 1181 128 499 414 248 35
Cuts at: 3031 480
Size: 2769
Fragments arranged by size:
2769 1181 499 414 248 128 37 35
9
BanII G_rGCy'C
Cuts at: 155 161 539 657 691 1288 1567 1807 2090
Size: 6 378 118 34 597 279 240 283
Cuts at: 2090 2332 3362 155
Size: 242 1030 2113
Fragments arranged by size:
2113 1030 597 378 283 279 242 240
118 34 6
BbeI G_GCGC'C
Cuts at: 2752 3000 2752
Size: 248 5072
BbsI GAAGACnn'nnnn_
Cuts at: 2084 2084
Size: 5320
BciVI GTATCCnnnnn_n'
Cuts at: 3216 3979 5320 3216
Size: 763 1341 3216
Fragments arranged by size:
3216 1341 763
BclI T'GATC_A
Cuts at: 2838 2838
Size: 5320
BfrBI ATG'CAT
Cuts at: 1992 1992
Size: 5320
BglII A'GATC_T
Cuts at: 2833 4872 2833
Size: 2039 3281
BlpI GC'TnA_GC
Cuts at: 253 1742 2430 253
Size: 1489 688 3143
Fragments arranged by size:
3143 1489 688
Bme1580I G_kGCm'C
Cuts at: 155 161 657 918 1051 1288 1567 1807 2943
Size: 6 496 261 133 237 279 240 1136
Cuts at: 2943 3036 4094 155
Size: 93 1058 1381
Fragments arranged by size:
1381 1136 1058 496 279 261 240 237
133 93 6
BmrI ACTGGGnnnn_n'
Cuts at: 158 237 1106 2530 2611 2946 5092 158
Size: 79 869 1424 81 335 2146 386
Fragments arranged by size:
2146 1424 869 386 335 81 79
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 835 3685 835
Size: 2850 2470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 392 2257 392
Size: 1865 3455
Bpu10I CC'TnA_GC
Cuts at: 538 538
Size: 5320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 372 2019 2494 3373 3867 4165 372
Size: 1647 475 879 494 298 1527
Fragments arranged by size:
1647 1527 879 494 475 298
BsaI GGTCTCn'nnnn_
Cuts at: 733 5024 733
Size: 4291 1029
BsaAI yAC'GTr
Cuts at: 2310 2671 2310
Size: 361 4959
BsaBI GATnn'nnATC
Cuts at: 6 2832 4871 6
Size: 2826 2039 455
Fragments arranged by size:
2826 2039 455
BsaHI Gr'CG_yC
Cuts at: 287 2749 2997 3701 5197 287
Size: 2462 248 704 1496 410
Fragments arranged by size:
2462 1496 704 410 248
BsaWI w'CCGG_w
Cuts at: 2507 3028 3708 3982 4129 4540 2507
Size: 521 680 274 147 411 3287
Fragments arranged by size:
3287 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 390 420 1402 1426 1432 1456 1470 1500 390
Size: 30 982 24 6 24 14 30 4210
Fragments arranged by size:
4210 982 30 30 24 24 14 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 573 573
Size: 5320
BseYI C'CCAG_C
Cuts at: 248 484 490 1088 1494 1680 1692 1752 2743
Size: 236 6 598 406 186 12 60 991
Cuts at: 2743 3501 4080 4576 248
Size: 758 579 496 992
Fragments arranged by size:
992 991 758 598 579 496 406 236
186 60 12 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1283 1523 1283
Size: 240 5080
BsiEI CG_ry'CG
Cuts at: 104 2860 2906 4116 104
Size: 2756 46 1210 1308
Fragments arranged by size:
2756 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 539 1051 1642 2332 3110 3300 4094 539
Size: 512 591 690 778 190 794 1765
Fragments arranged by size:
1765 794 778 690 591 512 190
Bsp1286I G_dGCh'C
Cuts at: 155 161 539 657 691 918 1051 1288 1567
Size: 6 378 118 34 227 133 237 279
Cuts at: 1567 1642 1807 2090 2332 2943 3036 3110 3300
Size: 75 165 283 242 611 93 74 190
Cuts at: 3300 3362 4094 155
Size: 62 732 1381
Fragments arranged by size:
1381 732 611 378 283 279 242 237
227 190 165 133 118 93 75 74
62 34 6
BspEI T'CCGG_A
Cuts at: 4540 4540
Size: 5320
BspHI T'CATG_A
Cuts at: 4496 4496
Size: 5320
BspMI ACCTGCnnnn'nnnn_
Cuts at: 941 1104 1266 1527 2029 2212 2368 2582 2884
Size: 163 162 261 502 183 156 214 302
Cuts at: 2884 3265 941
Size: 381 2996
Fragments arranged by size:
2996 502 381 302 261 214 183 163
162 156
BsrBI CCG'CTC
Cuts at: 50 454 3610 3664 5150 5315 50
Size: 404 3156 54 1486 165 55
Fragments arranged by size:
3156 1486 404 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3230 3230
Size: 5320
BsrFI r'CCGG_y
Cuts at: 426 1041 3316 3497 3708 426
Size: 615 2275 181 211 2038
Fragments arranged by size:
2275 2038 615 211 181
BssSI C'ACGA_G
Cuts at: 268 3589 3949 268
Size: 3321 360 1639
Fragments arranged by size:
3321 1639 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1665 2129 2695 1665
Size: 464 566 4290
Fragments arranged by size:
4290 566 464
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5320
BstEII G'GTnAC_C
Cuts at: 262 262
Size: 5320
BstXI CCAn_nnnn'nTGG
Cuts at: 1977 1977
Size: 5320
BstYI r'GATC_y
Cuts at: 1 2343 2810 2833 3168 3414 4428 4514 4526
Size: 2342 467 23 335 246 1014 86 12
Cuts at: 4526 4872 1
Size: 346 449
Fragments arranged by size:
2342 1014 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 497 628 745 1703 497
Size: 131 117 958 4114
Fragments arranged by size:
4114 958 131 117
BtgI C'CryG_G
Cuts at: 616 1820 2133 2150 616
Size: 1204 313 17 3786
Fragments arranged by size:
3786 1204 313 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1143 1938 3268 3449 3612 1143
Size: 795 1330 181 163 2851
Fragments arranged by size:
2851 1330 795 181 163
BtsI GCAGTG_nn'
Cuts at: 1151 1244 1322 1782 2561 1151
Size: 93 78 460 779 3910
Fragments arranged by size:
3910 779 460 93 78
DraI TTT'AAA
Cuts at: 2317 2317
Size: 5320
DraIII CAC_nnn'GTG
Cuts at: 2129 2129
Size: 5320
DrdI GACnn_nn'nnGTC
Cuts at: 3024 3884 3024
Size: 860 4460
EaeI y'GGCC_r
Cuts at: 275 1133 1241 2903 3077 3468 3495 4842 275
Size: 858 108 1662 174 391 27 1347 753
Fragments arranged by size:
1662 1347 858 753 391 174 108 27
EagI C'GGCC_G
Cuts at: 2903 2903
Size: 5320
EarI CTCTTCn'nnn_
Cuts at: 1227 1254 1838 1892 2071 3341 3551 1227
Size: 27 584 54 179 1270 210 2996
Fragments arranged by size:
2996 1270 584 210 179 54 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 564 1155 3526 3838 3984 5130 564
Size: 591 2371 312 146 1146 754
Fragments arranged by size:
2371 1146 754 591 312 146
EcoICRI GAG'CTC
Cuts at: 537 2330 537
Size: 1793 3527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 392 1235 1862 2257 3143 3575 4324 4592 392
Size: 843 627 395 886 432 749 268 1120
Fragments arranged by size:
1120 886 843 749 627 432 395 268
EcoO109I rG'GnC_Cy
Cuts at: 158 447 653 660 831 1211 1284 1285 1563
Size: 289 206 7 171 380 73 1 278
Cuts at: 1563 1564 1699 1803 2457 158
Size: 1 135 104 654 3021
Fragments arranged by size:
3021 654 380 289 278 206 171 135
104 73 7 1 1
EcoRI G'AATT_C
Cuts at: 979 2322 979
Size: 1343 3977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3337 3337
Size: 5320
FspI TGC'GCA
Cuts at: 2217 3099 2217
Size: 882 4438
HaeII r_GCGC'y
Cuts at: 851 2752 3000 4024 5003 851
Size: 1901 248 1024 979 1168
Fragments arranged by size:
1901 1168 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 390 422 835 867 1597 1629 3160 3190 3192
Size: 32 413 32 730 32 1531 30 2
Cuts at: 3192 3222 390
Size: 30 2488
Fragments arranged by size:
2488 1531 730 413 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 291 2357 291
Size: 2066 3254
KasI G'GCGC_C
Cuts at: 2748 2996 2748
Size: 248 5072
KpnI G_GTAC'C
Cuts at: 2338 2338
Size: 5320
MluI A'CGCG_T
Cuts at: 4753 4753
Size: 5320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 780 953 3991 4175 780
Size: 173 3038 184 1925
Fragments arranged by size:
3038 1925 184 173
MscI TGG'CCA
Cuts at: 277 1135 3079 277
Size: 858 1944 2518
Fragments arranged by size:
2518 1944 858
MslI CAynn'nnrTG
Cuts at: 3307 3434 3307
Size: 127 5193
MspA1I CmG'CkG
Cuts at: 618 1071 1141 1559 1696 1950 2429 2743 3103
Size: 453 70 418 137 254 479 314 360
Cuts at: 3103 4118 4363 618
Size: 1015 245 1575
Fragments arranged by size:
1575 1015 479 453 418 360 314 254
245 137 70
NaeI GCC'GGC
Cuts at: 3499 3499
Size: 5320
NarI GG'CG_CC
Cuts at: 2749 2997 2749
Size: 248 5072
NcoI C'CATG_G
Cuts at: 1820 2133 1820
Size: 313 5007
NgoMIV G'CCGG_C
Cuts at: 3497 3497
Size: 5320
NruI TCG'CGA
Cuts at: 3681 3681
Size: 5320
NsiI A_TGCA'T
Cuts at: 1994 1994
Size: 5320
NspI r_CATG'y
Cuts at: 201 1039 1996 2371 2871 3780 201
Size: 838 957 375 500 909 1741
Fragments arranged by size:
1741 957 909 838 500 375
PasI CC'CwG_GG
Cuts at: 161 1384 1592 2181 161
Size: 1223 208 589 3300
Fragments arranged by size:
3300 1223 589 208
PciI A'CATG_T
Cuts at: 3776 3776
Size: 5320
PfoI T'CCnGG_A
Cuts at: 214 214
Size: 5320
PmeI GTTT'AAAC
Cuts at: 2317 2317
Size: 5320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4459 4491 4459
Size: 32 5288
PpuMI rG'GwC_Cy
Cuts at: 447 660 831 1699 447
Size: 213 171 868 4068
Fragments arranged by size:
4068 868 213 171
PspOMI G'GGCC_C
Cuts at: 151 157 653 1284 1563 1803 151
Size: 6 496 631 279 240 3668
Fragments arranged by size:
3668 631 496 279 240 6
PspXI vC'TCGA_Gb
Cuts at: 690 690
Size: 5320
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1897 1929 1897
Size: 32 5288
PstI C_TGCA'G
Cuts at: 179 368 938 2365 3050 179
Size: 189 570 1427 685 2449
Fragments arranged by size:
2449 1427 685 570 189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5320
PvuII CAG'CTG
Cuts at: 1141 2743 3103 1141
Size: 1602 360 3358
Fragments arranged by size:
3358 1602 360
RsrII CG'GwC_CG
Cuts at: 3513 3513
Size: 5320
SacI G_AGCT'C
Cuts at: 539 2332 539
Size: 1793 3527
SacII CC_GC'GG
Cuts at: 619 619
Size: 5320
SalI G'TCGA_C
Cuts at: 2355 2355
Size: 5320
SapI GCTCTTCn'nnn_
Cuts at: 1892 3341 3551 1892
Size: 1449 210 3661
Fragments arranged by size:
3661 1449 210
SbfI CC_TGCA'GG
Cuts at: 2365 2365
Size: 5320
ScaI AGT'ACT
Cuts at: 4837 4837
Size: 5320
SfcI C'TryA_G
Cuts at: 33 175 364 934 1227 1275 1371 1554 2361
Size: 142 189 570 293 48 96 183 807
Cuts at: 2361 3046 4041 4232 33
Size: 685 995 191 1121
Fragments arranged by size:
1121 995 807 685 570 293 191 189
183 142 96 48
SfoI GGC'GCC
Cuts at: 2750 2998 2750
Size: 248 5072
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5320
SgrAI Cr'CCGG_yG
Cuts at: 1041 1041
Size: 5320
SmaI CCC'GGG
Cuts at: 156 658 1312 1631 1672 2340 156
Size: 502 654 319 41 668 3136
Fragments arranged by size:
3136 668 654 502 319 41
SmlI C'TyrA_G
Cuts at: 351 690 1998 2473 3388 3882 4144 351
Size: 339 1308 475 915 494 262 1527
Fragments arranged by size:
1527 1308 915 494 475 339 262
SnaBI TAC'GTA
Cuts at: 2310 2310
Size: 5320
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5320
SphI G_CATG'C
Cuts at: 1039 1996 2371 2871 1039
Size: 957 375 500 3488
Fragments arranged by size:
3488 957 500 375
SrfI GCCC'GGGC
Cuts at: 156 156
Size: 5320
StuI AGG'CCT
Cuts at: 598 790 1219 598
Size: 192 429 4699
Fragments arranged by size:
4699 429 192
StyI C'CwwG_G
Cuts at: 1207 1820 2133 2452 2806 1207
Size: 613 313 319 354 3721
Fragments arranged by size:
3721 613 354 319 313
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 498 1279 1317 1584 2901 3034 3493 3704 498
Size: 781 38 267 1317 133 459 211 2114
Fragments arranged by size:
2114 1317 781 459 267 211 133 38
TatI w'GTAC_w
Cuts at: 728 4835 728
Size: 4107 1213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 395 427 1470 1502 4837 4869 395
Size: 32 1043 32 3335 32 846
Fragments arranged by size:
3335 1043 846 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 286 3115 286
Size: 2829 2491
XbaI T'CTAG_A
Cuts at: 2349 2349
Size: 5320
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 309 2813 309
Size: 2504 2816
XhoI C'TCGA_G
Cuts at: 690 690
Size: 5320
XmaI C'CCGG_G
Cuts at: 154 656 1310 1629 1670 2338 154
Size: 502 654 319 41 668 3136
Fragments arranged by size:
3136 668 654 502 319 41
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI
NspI PasI PciI PfoI PmeI PpiI PpuMI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
PacI PflMI PmlI PshAI PsiI SanDI SexAI SfiI
SspI SwaI XmnI ZraI