Restriction map for
pF1KSDA0615
(Circular) (Six-base) MAPSORT of: pF1KSDA0615.seq Check: 4127 from: 1 to: 5827
pF1KSDA0615 5827 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 2863 2863
Size: 5827
Acc65I G'GTAC_C
Cuts at: 2841 2841
Size: 5827
AclI AA'CG_TT
Cuts at: 1758 5667 1758
Size: 3909 1918
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1016 2422 2499 2731 3650 4082 4831 5099 1016
Size: 1406 77 232 919 432 749 268 1744
Fragments arranged by size:
1744 1406 919 749 432 268 232 77
AflIII A'CryG_T
Cuts at: 2363 3177 4283 5260 2363
Size: 814 1106 977 2930
Fragments arranged by size:
2930 1106 977 814
AgeI A'CCGG_T
Cuts at: 4215 4215
Size: 5827
AleI CACnn'nnGTG
Cuts at: 2368 2368
Size: 5827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 298 298
Size: 5827
AlwNI CAG_nnn'CTG
Cuts at: 1585 1897 2652 3130 4699 1585
Size: 312 755 478 1569 2713
Fragments arranged by size:
2713 1569 755 478 312
ApaLI G'TGCA_C
Cuts at: 4597 4597
Size: 5827
ApoI r'AATT_y
Cuts at: 601 965 1122 1312 1447 2305 2829 5180 5436
Size: 364 157 190 135 858 524 2351 256
Cuts at: 5436 601
Size: 992
Fragments arranged by size:
2351 992 858 524 364 256 190 157
135
AseI AT'TA_AT
Cuts at: 20 1505 20
Size: 1485 4342
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5827
AvaI C'yCGr_G
Cuts at: 134 2845 134
Size: 2711 3116
BamHI G'GATC_C
Cuts at: 2850 2850
Size: 5827
BanI G'GyrC_C
Cuts at: 2527 2841 3255 3503 3538 2527
Size: 314 414 248 35 4816
Fragments arranged by size:
4816 414 314 248 35
BanII G_rGCy'C
Cuts at: 2177 2580 2839 3869 2177
Size: 403 259 1030 4135
Fragments arranged by size:
4135 1030 403 259
BbeI G_GCGC'C
Cuts at: 3259 3507 3259
Size: 248 5579
BbsI GAAGACnn'nnnn_
Cuts at: 1310 1310
Size: 5827
BbvCI CC'TCA_GC
Cuts at: 254 497 2600 2695 254
Size: 243 2103 95 3386
Fragments arranged by size:
3386 2103 243 95
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 139 173 139
Size: 34 5793
BciVI GTATCCnnnnn_n'
Cuts at: 432 1343 3723 4486 5827 432
Size: 911 2380 763 1341 432
Fragments arranged by size:
2380 1341 911 763 432
BclI T'GATC_A
Cuts at: 1875 3345 1875
Size: 1470 4357
BglI GCCn_nnn'nGGC
Cuts at: 218 479 218
Size: 261 5566
BglII A'GATC_T
Cuts at: 2671 2714 2753 2781 3340 5379 2671
Size: 43 39 28 559 2039 3119
Fragments arranged by size:
3119 2039 559 43 39 28
BlpI GC'TnA_GC
Cuts at: 2937 2937
Size: 5827
Bme1580I G_kGCm'C
Cuts at: 2484 3450 3543 4601 2484
Size: 966 93 1058 3710
Fragments arranged by size:
3710 1058 966 93
BmrI ACTGGGnnnn_n'
Cuts at: 563 1907 2630 3037 3118 3453 5599 563
Size: 1344 723 407 81 335 2146 791
Fragments arranged by size:
2146 1344 791 723 407 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 770 2340 4192 770
Size: 1570 1852 2405
Fragments arranged by size:
2405 1852 1570
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 2152 2626 205
Size: 1947 474 3406
Fragments arranged by size:
3406 1947 474
Bpu10I CC'TnA_GC
Cuts at: 254 497 2600 2695 254
Size: 243 2103 95 3386
Fragments arranged by size:
3386 2103 243 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3001 3880 4374 4672 3001
Size: 879 494 298 4156
Fragments arranged by size:
4156 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1333 5531 1333
Size: 4198 1629
BsaAI yAC'GTr
Cuts at: 2817 3178 2817
Size: 361 5466
BsaBI GATnn'nnATC
Cuts at: 6 3339 5378 6
Size: 3333 2039 455
Fragments arranged by size:
3333 2039 455
BsaHI Gr'CG_yC
Cuts at: 3256 3504 4208 5704 3256
Size: 248 704 1496 3379
Fragments arranged by size:
3379 1496 704 248
BsaWI w'CCGG_w
Cuts at: 3014 3535 4215 4489 4636 5047 3014
Size: 521 680 274 147 411 3794
Fragments arranged by size:
3794 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1794 1824 1794
Size: 30 5797
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 271 718 1769 2443 271
Size: 447 1051 674 3655
Fragments arranged by size:
3655 1051 674 447
BseYI C'CCAG_C
Cuts at: 844 1829 2162 2509 3250 4008 4587 5083 844
Size: 985 333 347 741 758 579 496 1588
Fragments arranged by size:
1588 985 758 741 579 496 347 333
BsiEI CG_ry'CG
Cuts at: 104 201 3367 3413 4623 104
Size: 97 3166 46 1210 1308
Fragments arranged by size:
3166 1308 1210 97 46
BsiHKAI G_wGCw'C
Cuts at: 239 314 2177 2839 3617 3807 4601 239
Size: 75 1863 662 778 190 794 1465
Fragments arranged by size:
1863 1465 794 778 662 190 75
BsmI GAATG_Cn'
Cuts at: 458 783 458
Size: 325 5502
Bsp1286I G_dGCh'C
Cuts at: 239 314 2177 2484 2580 2839 3450 3543 3617
Size: 75 1863 307 96 259 611 93 74
Cuts at: 3617 3807 3869 4601 239
Size: 190 62 732 1465
Fragments arranged by size:
1863 1465 732 611 307 259 190 96
93 75 74 62
BspEI T'CCGG_A
Cuts at: 5047 5047
Size: 5827
BspHI T'CATG_A
Cuts at: 2232 5003 2232
Size: 2771 3056
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2660 2875 3089 3391 3772 2660
Size: 215 214 302 381 4715
Fragments arranged by size:
4715 381 302 215 214
BsrBI CCG'CTC
Cuts at: 50 4117 4171 5657 5822 50
Size: 4067 54 1486 165 55
Fragments arranged by size:
4067 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2007 3737 2007
Size: 1730 4097
BsrFI r'CCGG_y
Cuts at: 3823 4004 4215 3823
Size: 181 211 5435
Fragments arranged by size:
5435 211 181
BssHII G'CGCG_C
Cuts at: 242 271 242
Size: 29 5798
BssSI C'ACGA_G
Cuts at: 4096 4456 4096
Size: 360 5467
BstAPI GCAn_nnn'nTGC
Cuts at: 2652 3202 2652
Size: 550 5277
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5827
BstXI CCAn_nnnn'nTGG
Cuts at: 2808 2808
Size: 5827
BstYI r'GATC_y
Cuts at: 1 2671 2714 2753 2781 2850 3317 3340 3675
Size: 2670 43 39 28 69 467 23 335
Cuts at: 3675 3921 4935 5021 5033 5379 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
2670 1014 467 449 346 335 246 86
69 43 39 28 23 12
BtgI C'CryG_G
Cuts at: 125 195 1193 2016 2801 125
Size: 70 998 823 785 3151
Fragments arranged by size:
3151 998 823 785 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3775 3956 4119 3775
Size: 181 163 5483
Fragments arranged by size:
5483 181 163
BtsI GCAGTG_nn'
Cuts at: 155 387 1822 2617 3068 155
Size: 232 1435 795 451 2914
Fragments arranged by size:
2914 1435 795 451 232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2253 2288 2253
Size: 35 5792
DraI TTT'AAA
Cuts at: 1188 2824 1188
Size: 1636 4191
DraIII CAC_nnn'GTG
Cuts at: 700 700
Size: 5827
DrdI GACnn_nn'nnGTC
Cuts at: 3531 4391 3531
Size: 860 4967
EaeI y'GGCC_r
Cuts at: 198 3410 3584 3975 4002 5349 198
Size: 3212 174 391 27 1347 676
Fragments arranged by size:
3212 1347 676 391 174 27
EagI C'GGCC_G
Cuts at: 198 3410 198
Size: 3212 2615
EarI CTCTTCn'nnn_
Cuts at: 821 938 3848 4058 821
Size: 117 2910 210 2590
Fragments arranged by size:
2910 2590 210 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 190 322 4033 4345 4491 5637 190
Size: 132 3711 312 146 1146 380
Fragments arranged by size:
3711 1146 380 312 146 132
EcoICRI GAG'CTC
Cuts at: 2175 2837 2175
Size: 662 5165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 1016 2152 2422 2499 2626 2731 3650 4082
Size: 811 1136 270 77 127 105 919 432
Cuts at: 4082 4831 5099 205
Size: 749 268 933
Fragments arranged by size:
1136 933 919 811 749 432 270 268
127 105 77
EcoO109I rG'GnC_Cy
Cuts at: 210 1867 2964 210
Size: 1657 1097 3073
Fragments arranged by size:
3073 1657 1097
EcoRI G'AATT_C
Cuts at: 601 2829 601
Size: 2228 3599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 967 1673 2243 2470 3844 967
Size: 706 570 227 1374 2950
Fragments arranged by size:
2950 1374 706 570 227
FspI TGC'GCA
Cuts at: 3606 3606
Size: 5827
HaeII r_GCGC'y
Cuts at: 320 3259 3507 4531 5510 320
Size: 2939 248 1024 979 637
Fragments arranged by size:
2939 1024 979 637 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 954 986 1843 1875 3667 3697 3699 3729 954
Size: 32 857 32 1792 30 2 30 3052
Fragments arranged by size:
3052 1792 857 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2864 2864
Size: 5827
HindIII A'AGCT_T
Cuts at: 426 2077 426
Size: 1651 4176
KasI G'GCGC_C
Cuts at: 3255 3503 3255
Size: 248 5579
KpnI G_GTAC'C
Cuts at: 2845 2845
Size: 5827
MfeI C'AATT_G
Cuts at: 2054 2054
Size: 5827
MluI A'CGCG_T
Cuts at: 5260 5260
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2584 2789 4498 4682 2584
Size: 205 1709 184 3729
Fragments arranged by size:
3729 1709 205 184
MscI TGG'CCA
Cuts at: 3586 3586
Size: 5827
MslI CAynn'nnrTG
Cuts at: 1231 2281 2368 2383 3814 3941 1231
Size: 1050 87 15 1431 127 3117
Fragments arranged by size:
3117 1431 1050 127 87 15
MspA1I CmG'CkG
Cuts at: 166 197 264 1321 1833 2936 3250 3610 4625
Size: 31 67 1057 512 1103 314 360 1015
Cuts at: 4625 4870 166
Size: 245 1123
Fragments arranged by size:
1123 1103 1057 1015 512 360 314 245
67 31
NaeI GCC'GGC
Cuts at: 4006 4006
Size: 5827
NarI GG'CG_CC
Cuts at: 3256 3504 3256
Size: 248 5579
NcoI C'CATG_G
Cuts at: 125 1193 2016 2801 125
Size: 1068 823 785 3151
Fragments arranged by size:
3151 1068 823 785
NgoMIV G'CCGG_C
Cuts at: 4004 4004
Size: 5827
NruI TCG'CGA
Cuts at: 4188 4188
Size: 5827
NspI r_CATG'y
Cuts at: 387 2502 2878 3378 4287 387
Size: 2115 376 500 909 1927
Fragments arranged by size:
2115 1927 909 500 376
PciI A'CATG_T
Cuts at: 4283 4283
Size: 5827
PflMI CCAn_nnn'nTGG
Cuts at: 925 2757 925
Size: 1832 3995
PfoI T'CCnGG_A
Cuts at: 2168 2168
Size: 5827
PmeI GTTT'AAAC
Cuts at: 2824 2824
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4966 4998 4966
Size: 32 5795
PpuMI rG'GwC_Cy
Cuts at: 1867 1867
Size: 5827
PstI C_TGCA'G
Cuts at: 287 2360 2682 2872 3557 287
Size: 2073 322 190 685 2557
Fragments arranged by size:
2557 2073 685 322 190
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5827
PvuII CAG'CTG
Cuts at: 166 1321 1833 3250 3610 166
Size: 1155 512 1417 360 2383
Fragments arranged by size:
2383 1417 1155 512 360
RsrII CG'GwC_CG
Cuts at: 4020 4020
Size: 5827
SacI G_AGCT'C
Cuts at: 2177 2839 2177
Size: 662 5165
SacII CC_GC'GG
Cuts at: 198 198
Size: 5827
SalI G'TCGA_C
Cuts at: 2862 2862
Size: 5827
SapI GCTCTTCn'nnn_
Cuts at: 3848 4058 3848
Size: 210 5617
SbfI CC_TGCA'GG
Cuts at: 2872 2872
Size: 5827
ScaI AGT'ACT
Cuts at: 5344 5344
Size: 5827
SfcI C'TryA_G
Cuts at: 33 283 2356 2678 2868 3553 4548 4739 33
Size: 250 2073 322 190 685 995 191 1121
Fragments arranged by size:
2073 1121 995 685 322 250 191 190
SfoI GGC'GCC
Cuts at: 3257 3505 3257
Size: 248 5579
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5827
SmaI CCC'GGG
Cuts at: 136 2847 136
Size: 2711 3116
SmlI C'TyrA_G
Cuts at: 2980 3895 4389 4651 2980
Size: 915 494 262 4156
Fragments arranged by size:
4156 915 494 262
SnaBI TAC'GTA
Cuts at: 2817 2817
Size: 5827
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5827
SphI G_CATG'C
Cuts at: 2878 3378 2878
Size: 500 5327
SrfI GCCC'GGGC
Cuts at: 136 136
Size: 5827
SspI AAT'ATT
Cuts at: 798 2068 2183 798
Size: 1270 115 4442
Fragments arranged by size:
4442 1270 115
StyI C'CwwG_G
Cuts at: 125 320 377 1193 2016 2801 2959 3313 125
Size: 195 57 816 823 785 158 354 2639
Fragments arranged by size:
2639 823 816 785 354 195 158 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2545 2783 3408 3541 4000 4211 2545
Size: 238 625 133 459 211 4161
Fragments arranged by size:
4161 625 459 238 211 133
TatI w'GTAC_w
Cuts at: 2360 5342 2360
Size: 2982 2845
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5344 5376 5344
Size: 32 5795
Tth111I GACn'n_nGTC
Cuts at: 883 3622 883
Size: 2739 3088
XbaI T'CTAG_A
Cuts at: 802 2856 802
Size: 2054 3773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3320 3320
Size: 5827
XmaI C'CCGG_G
Cuts at: 134 2845 134
Size: 2711 3116
XmnI GAAnn'nnTTC
Cuts at: 1034 2029 2226 2705 2716 1034
Size: 995 197 479 11 4145
Fragments arranged by size:
4145 995 479 197 11
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BfrBI BmgBI BmtI BsgI BsiWI BsmBI
BsrGI BstEII BstZ17I Bsu36I ClaI EcoNI EcoRV FseI
FspAI HpaI NdeI NheI NotI NsiI PacI PasI
PmlI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI StuI SwaI XhoI ZraI