Restriction map for
pF1KSDA0620
(Circular) (Six-base) MAPSORT of: pF1KSDA0620.seq Check: 967 from: 1 to: 8912
pF1KSDA0620 8912 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1481 1626 2794 3502 5841 1481
Size: 145 1168 708 2339 4552
Fragments arranged by size:
4552 2339 1168 708 145
AatII G_ACGT'C
Cuts at: 1738 4943 1738
Size: 3205 5707
AccI GT'mk_AC
Cuts at: 4943 5950 4943
Size: 1007 7905
Acc65I G'GTAC_C
Cuts at: 5928 5928
Size: 8912
AclI AA'CG_TT
Cuts at: 8752 8752
Size: 8912
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1525 5081 5473 6737 7169 7916 8184 1525
Size: 3556 392 1264 432 747 268 2253
Fragments arranged by size:
3556 2253 1264 747 432 392 268
AfeI AGC'GCT
Cuts at: 5635 5635
Size: 8912
AflIII A'CryG_T
Cuts at: 689 1013 4802 6264 7368 8345 689
Size: 324 3789 1462 1104 977 1256
Fragments arranged by size:
3789 1462 1256 1104 977 324
AgeI A'CCGG_T
Cuts at: 766 7300 766
Size: 6534 2378
AleI CACnn'nnGTG
Cuts at: 1516 1543 2321 5005 5737 1516
Size: 27 778 2684 732 4691
Fragments arranged by size:
4691 2684 778 732 27
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 760 1108 2002 2131 3985 4924 5233 760
Size: 348 894 129 1854 939 309 4439
Fragments arranged by size:
4439 1854 939 894 348 309 129
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 114 146 5272 5304 114
Size: 32 5126 32 3722
Fragments arranged by size:
5126 3722 32 32
AlwNI CAG_nnn'CTG
Cuts at: 531 1503 1922 2080 2703 3168 3394 4588 6217
Size: 972 419 158 623 465 226 1194 1629
Cuts at: 6217 7784 531
Size: 1567 1659
Fragments arranged by size:
1659 1629 1567 1194 972 623 465 419
226 158
ApaI G_GGCC'C
Cuts at: 415 1915 3100 3246 3513 415
Size: 1500 1185 146 267 5814
Fragments arranged by size:
5814 1500 1185 267 146
ApaLI G'TGCA_C
Cuts at: 1001 1681 1777 1848 3295 7682 1001
Size: 680 96 71 1447 4387 2231
Fragments arranged by size:
4387 2231 1447 680 96 71
ApoI r'AATT_y
Cuts at: 5916 8265 8521 5916
Size: 2349 256 6307
Fragments arranged by size:
6307 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8912
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8912
AvaI C'yCGr_G
Cuts at: 164 832 1217 1530 1995 2066 2797 3041 3505
Size: 668 385 313 465 71 731 244 464
Cuts at: 3505 3601 3697 5932 164
Size: 96 96 2235 3144
Fragments arranged by size:
3144 2235 731 668 465 464 385 313
244 96 96 71
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2233 2266 2233
Size: 33 8879
BamHI G'GATC_C
Cuts at: 5937 5937
Size: 8912
BanI G'GyrC_C
Cuts at: 209 216 259 376 417 479 578 754 934
Size: 7 43 117 41 62 99 176 180
Cuts at: 934 1102 1359 2406 2442 2782 3112 3258 5065
Size: 168 257 1047 36 340 330 146 1807
Cuts at: 5065 5928 6342 6590 6625 209
Size: 863 414 248 35 2496
Fragments arranged by size:
2496 1807 1047 863 414 340 330 257
248 180 176 168 146 117 99 62
43 41 36 35 7
BanII G_rGCy'C
Cuts at: 415 641 1679 1915 2371 2648 2819 3100 3161
Size: 226 1038 236 456 277 171 281 61
Cuts at: 3161 3246 3458 3513 3749 4711 5183 5926 6956
Size: 85 212 55 236 962 472 743 1030
Cuts at: 6956 415
Size: 2371
Fragments arranged by size:
2371 1038 1030 962 743 472 456 281
277 236 236 226 212 171 85 61
55
BbeI G_GCGC'C
Cuts at: 263 380 483 582 758 938 1106 1363 6346
Size: 117 103 99 176 180 168 257 4983
Cuts at: 6346 6594 263
Size: 248 2581
Fragments arranged by size:
4983 2581 257 248 180 176 168 117
103 99
BbsI GAAGACnn'nnnn_
Cuts at: 1200 1230 1572 2720 3993 4048 5021 5321 5419
Size: 30 342 1148 1273 55 973 300 98
Cuts at: 5419 1200
Size: 4693
Fragments arranged by size:
4693 1273 1148 973 342 300 98 55
30
BbvCI CC'TCA_GC
Cuts at: 3584 3584
Size: 8912
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4083 4117 5188 5222 4083
Size: 34 1071 34 7773
Fragments arranged by size:
7773 1071 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5317 6810 7571 8912 5317
Size: 1493 761 1341 5317
Fragments arranged by size:
5317 1493 1341 761
BclI T'GATC_A
Cuts at: 6432 6432
Size: 8912
BglI GCCn_nnn'nGGC
Cuts at: 403 2018 3250 5074 5695 403
Size: 1615 1232 1824 621 3620
Fragments arranged by size:
3620 1824 1615 1232 621
BglII A'GATC_T
Cuts at: 1991 2507 4128 6427 8464 1991
Size: 516 1621 2299 2037 2439
Fragments arranged by size:
2439 2299 2037 1621 516
BlpI GC'TnA_GC
Cuts at: 2596 4025 4106 4834 5668 6024 2596
Size: 1429 81 728 834 356 5484
Fragments arranged by size:
5484 1429 834 728 356 81
Bme1580I G_kGCm'C
Cuts at: 214 415 422 1005 1402 1591 1685 1781 1836
Size: 201 7 583 397 189 94 96 55
Cuts at: 1836 1852 1915 3100 3115 3246 3299 3513 4913
Size: 16 63 1185 15 131 53 214 1400
Cuts at: 4913 5263 6537 6630 7686 214
Size: 350 1274 93 1056 1440
Fragments arranged by size:
1440 1400 1274 1185 1056 583 397 350
214 201 189 131 96 94 93 63
55 53 16 15 7
BmgBI CAC'GTC
Cuts at: 1311 1368 2892 4021 1311
Size: 57 1524 1129 6202
Fragments arranged by size:
6202 1524 1129 57
BmrI ACTGGGnnnn_n'
Cuts at: 2691 4914 5119 5457 6124 6205 6540 8684 2691
Size: 2223 205 338 667 81 335 2144 2919
Fragments arranged by size:
2919 2223 2144 667 338 335 205 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7279 7279
Size: 8912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 412 826 1423 1858 2503 2521 3103 3145
Size: 126 414 597 435 645 18 582 42
Cuts at: 3145 3733 4192 4435 4513 4909 4947 5377 5821
Size: 588 459 243 78 396 38 430 444
Cuts at: 5821 286
Size: 3377
Fragments arranged by size:
3377 645 597 588 582 459 444 435
430 414 396 243 126 78 42 38
18
Bpu10I CC'TnA_GC
Cuts at: 157 385 2642 3514 3584 157
Size: 228 2257 872 70 5485
Fragments arranged by size:
5485 2257 872 228 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 1594 4969 6088 6967 7459 7757 904
Size: 690 3375 1119 879 492 298 2059
Fragments arranged by size:
3375 2059 1119 879 690 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 8616 8616
Size: 8912
BsaAI yAC'GTr
Cuts at: 761 2160 3060 4239 5904 6265 761
Size: 1399 900 1179 1665 361 3408
Fragments arranged by size:
3408 1665 1399 1179 900 361
BsaBI GATnn'nnATC
Cuts at: 6 841 3124 4621 6426 8463 6
Size: 835 2283 1497 1805 2037 455
Fragments arranged by size:
2283 2037 1805 1497 835 455
BsaHI Gr'CG_yC
Cuts at: 199 260 377 480 579 755 935 1103 1115
Size: 61 117 103 99 176 180 168 12
Cuts at: 1115 1360 1735 1814 4541 4718 4940 5501 5537
Size: 245 375 79 2727 177 222 561 36
Cuts at: 5537 5662 6343 6591 7293 8789 199
Size: 125 681 248 702 1496 322
Fragments arranged by size:
2727 1496 702 681 561 375 322 248
245 222 180 177 176 168 125 117
103 99 79 61 36 12
BsaWI w'CCGG_w
Cuts at: 766 3255 5432 5618 6101 6622 7300 7574 7721
Size: 2489 2177 186 483 521 678 274 147
Cuts at: 7721 8132 766
Size: 411 1546
Fragments arranged by size:
2489 2177 1546 678 521 483 411 274
186 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 114 144 763 793 1765 1795 1831 1861 2953
Size: 30 619 30 972 30 36 30 1092
Cuts at: 2953 2983 3022 3037 3052 3067 4693 4723 5527
Size: 30 39 15 15 15 1626 30 804
Cuts at: 5527 5557 114
Size: 30 3469
Fragments arranged by size:
3469 1626 1092 972 804 619 39 36
30 30 30 30 30 30 30 15
15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 122 2518 2974 3601 3619 4075 4356 4768 5374
Size: 2396 456 627 18 456 281 412 606
Cuts at: 5374 5581 5716 122
Size: 207 135 3318
Fragments arranged by size:
3318 2396 627 606 456 456 412 281
207 135 18
BseYI C'CCAG_C
Cuts at: 655 954 1212 1704 1915 2008 3918 4302 5562
Size: 299 258 492 211 93 1910 384 1260
Cuts at: 5562 6337 7095 7672 8168 655
Size: 775 758 577 496 1399
Fragments arranged by size:
1910 1399 1260 775 758 577 496 492
384 299 258 211 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 1414 1851 2225 2325 2495 2548 3174 3210
Size: 453 437 374 100 170 53 626 36
Cuts at: 3210 3436 4465 4470 4952 5650 961
Size: 226 1029 5 482 698 4223
Fragments arranged by size:
4223 1029 698 626 482 453 437 374
226 170 100 53 36 5
BsiEI CG_ry'CG
Cuts at: 104 350 407 599 1283 3854 4550 5022 5782
Size: 246 57 192 684 2571 696 472 760
Cuts at: 5782 6454 6500 7708 104
Size: 672 46 1208 1308
Fragments arranged by size:
2571 1308 1208 760 696 684 672 472
246 192 57 46
BsiHKAI G_wGCw'C
Cuts at: 473 1005 1382 1685 1781 1852 3161 3299 5222
Size: 532 377 303 96 71 1309 138 1923
Cuts at: 5222 5926 6704 6894 7686 473
Size: 704 778 190 792 1699
Fragments arranged by size:
1923 1699 1309 792 778 704 532 377
303 190 138 96 71
BsmI GAATG_Cn'
Cuts at: 5691 5691
Size: 8912
BsmBI CGTCTCn'nnnn_
Cuts at: 1834 3925 4642 1834
Size: 2091 717 6104
Fragments arranged by size:
6104 2091 717
Bsp1286I G_dGCh'C
Cuts at: 214 415 422 473 641 1005 1382 1402 1591
Size: 201 7 51 168 364 377 20 189
Cuts at: 1591 1679 1685 1781 1836 1852 1915 2371 2648
Size: 88 6 96 55 16 63 456 277
Cuts at: 2648 2819 3100 3115 3161 3246 3299 3458 3513
Size: 171 281 15 46 85 53 159 55
Cuts at: 3513 3749 4711 4913 5183 5222 5263 5926 6537
Size: 236 962 202 270 39 41 663 611
Cuts at: 6537 6630 6704 6894 6956 7686 214
Size: 93 74 190 62 730 1440
Fragments arranged by size:
1440 962 730 663 611 456 377 364
281 277 270 236 202 201 190 189
171 168 159 96 93 88 85 74
63 62 55 55 53 51 46 41
39 20 16 15 7 6
BspEI T'CCGG_A
Cuts at: 8132 8132
Size: 8912
BspHI T'CATG_A
Cuts at: 4511 8088 4511
Size: 3577 5335
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1481 1626 2794 3502 3614 5564 5841 5962 6176
Size: 145 1168 708 112 1950 277 121 214
Cuts at: 6176 6478 6859 1481
Size: 302 381 3534
Fragments arranged by size:
3534 1950 1168 708 381 302 277 214
145 121 112
BsrBI CCG'CTC
Cuts at: 50 848 1224 1245 2595 5667 7204 7258 8742
Size: 798 376 21 1350 3072 1537 54 1484
Cuts at: 8742 8907 50
Size: 165 55
Fragments arranged by size:
3072 1537 1484 1350 798 376 165 55
54 21
BsrDI GCAATG_nn'
Cuts at: 6824 6824
Size: 8912
BsrFI r'CCGG_y
Cuts at: 256 766 1105 1356 1407 2876 3249 4331 5195
Size: 510 339 251 51 1469 373 1082 864
Cuts at: 5195 6910 7091 7300 256
Size: 1715 181 209 1868
Fragments arranged by size:
1868 1715 1469 1082 864 510 373 339
251 209 181 51
BsrGI T'GTAC_A
Cuts at: 4631 4631
Size: 8912
BssHII G'CGCG_C
Cuts at: 1039 1041 1081 1333 1039
Size: 2 40 252 8618
Fragments arranged by size:
8618 252 40 2
BssSI C'ACGA_G
Cuts at: 462 748 2364 3757 7183 7541 462
Size: 286 1616 1393 3426 358 1833
Fragments arranged by size:
3426 1833 1616 1393 358 286
BstAPI GCAn_nnn'nTGC
Cuts at: 1343 3224 6289 1343
Size: 1881 3065 3966
Fragments arranged by size:
3966 3065 1881
BstBI TT'CG_AA
Cuts at: 110 7273 110
Size: 7163 1749
BstEII G'GTnAC_C
Cuts at: 2089 2722 3009 2089
Size: 633 287 7992
Fragments arranged by size:
7992 633 287
BstXI CCAn_nnnn'nTGG
Cuts at: 3390 3445 4819 3390
Size: 55 1374 7483
Fragments arranged by size:
7483 1374 55
BstYI r'GATC_y
Cuts at: 1 269 503 1991 2507 2801 4076 4128 4883
Size: 268 234 1488 516 294 1275 52 755
Cuts at: 4883 5054 5648 5937 6404 6427 6762 7008 8020
Size: 171 594 289 467 23 335 246 1012
Cuts at: 8020 8106 8118 8464 1
Size: 86 12 346 449
Fragments arranged by size:
1488 1275 1012 755 594 516 467 449
346 335 294 289 268 246 234 171
86 52 23 12
Bsu36I CC'TnA_GG
Cuts at: 2372 3236 2372
Size: 864 8048
BtgI C'CryG_G
Cuts at: 125 140 205 407 475 599 698 722 1098
Size: 15 65 202 68 124 99 24 376
Cuts at: 1098 1176 1262 1544 1550 2675 2774 2898 3092
Size: 78 86 282 6 1125 99 124 194
Cuts at: 3092 3129 3428 3854 3983 4847 5744 5813 125
Size: 37 299 426 129 864 897 69 3224
Fragments arranged by size:
3224 1125 897 864 426 376 299 282
202 194 129 124 124 99 99 86
78 69 68 65 37 24 15 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3204 3888 4458 5191 5502 6862 7043 7206 3204
Size: 684 570 733 311 1360 181 163 4910
Fragments arranged by size:
4910 1360 733 684 570 311 181 163
BtsI GCAGTG_nn'
Cuts at: 1162 2237 2456 2932 3822 3910 4297 6155 1162
Size: 1075 219 476 890 88 387 1858 3919
Fragments arranged by size:
3919 1858 1075 890 476 387 219 88
ClaI AT'CG_AT
Cuts at: 1955 1955
Size: 8912
DraI TTT'AAA
Cuts at: 5911 5911
Size: 8912
DraIII CAC_nnn'GTG
Cuts at: 2904 3414 4188 2904
Size: 510 774 7628
Fragments arranged by size:
7628 774 510
DrdI GACnn_nn'nnGTC
Cuts at: 6618 7476 6618
Size: 858 8054
EaeI y'GGCC_r
Cuts at: 347 404 522 596 602 674 872 1280 1547
Size: 57 118 74 6 72 198 408 267
Cuts at: 1547 1553 1748 2450 2617 3341 3431 3447 3665
Size: 6 195 702 167 724 90 16 218
Cuts at: 3665 3851 3935 4547 4924 5019 5075 5696 5741
Size: 186 84 612 377 95 56 621 45
Cuts at: 5741 5779 5792 6497 6671 7062 7089 8434 347
Size: 38 13 705 174 391 27 1345 825
Fragments arranged by size:
1345 825 724 705 702 621 612 408
391 377 267 218 198 195 186 174
167 118 95 90 84 74 72 57
56 45 38 27 16 13 6 6
EagI C'GGCC_G
Cuts at: 347 404 596 1280 3851 4547 5019 5779 6497
Size: 57 192 684 2571 696 472 760 718
Cuts at: 6497 347
Size: 2762
Fragments arranged by size:
2762 2571 760 718 696 684 472 192
57
EarI CTCTTCn'nnn_
Cuts at: 886 2572 4223 4340 5368 6935 7145 886
Size: 1686 1651 117 1028 1567 210 2653
Fragments arranged by size:
2653 1686 1651 1567 1028 210 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1825 2123 2208 3619 5191 7120 7430 7576 8722
Size: 298 85 1411 1572 1929 310 146 1146
Cuts at: 8722 1825
Size: 2015
Fragments arranged by size:
2015 1929 1572 1411 1146 310 298 146
85
EcoICRI GAG'CTC
Cuts at: 3159 5924 3159
Size: 2765 6147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 412 826 1423 1525 1858 2503 2521 3103
Size: 126 414 597 102 333 645 18 582
Cuts at: 3103 3145 3733 4192 4435 4513 4909 4947 5081
Size: 42 588 459 243 78 396 38 134
Cuts at: 5081 5377 5473 5821 6737 7169 7916 8184 286
Size: 296 96 348 916 432 747 268 1014
Fragments arranged by size:
1014 916 747 645 597 588 582 459
432 414 396 348 333 296 268 243
134 126 102 96 78 42 38 18
EcoNI CCTnn'n_nnAGG
Cuts at: 5290 5290
Size: 8912
EcoO109I rG'GnC_Cy
Cuts at: 528 1256 1911 1912 2070 2077 2761 2843 3097
Size: 728 655 1 158 7 684 82 254
Cuts at: 3097 3165 3242 3243 3509 3510 4989 6051 528
Size: 68 77 1 266 1 1479 1062 3389
Fragments arranged by size:
3389 1479 1062 728 684 655 266 254
158 82 77 68 7 1 1 1
EcoRI G'AATT_C
Cuts at: 5916 5916
Size: 8912
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 975 6931 975
Size: 5956 2956
FspI TGC'GCA
Cuts at: 3205 6693 3205
Size: 3488 5424
FspAI rTGC'GCAy
Cuts at: 3205 3205
Size: 8912
HaeII r_GCGC'y
Cuts at: 164 263 380 483 582 758 938 971 1027
Size: 99 117 103 99 176 180 33 56
Cuts at: 1027 1106 1363 3532 5637 6346 6594 7616 8595
Size: 79 257 2169 2105 709 248 1022 979
Cuts at: 8595 164
Size: 481
Fragments arranged by size:
2169 2105 1022 979 709 481 257 248
180 176 117 103 99 99 79 56
33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 261 293 1307 1339 3022 3037 3054 3069 4054
Size: 32 1014 32 1683 15 17 15 985
Cuts at: 4054 4056 4073 4086 4088 4105 4987 5019 5527
Size: 2 17 13 2 17 882 32 508
Cuts at: 5527 5559 6754 6784 6786 6816 261
Size: 32 1195 30 2 30 2357
Fragments arranged by size:
2357 1683 1195 1014 985 882 508 32
32 32 32 30 30 17 17 17
15 15 13 2 2 2
HincII GTy'rAC
Cuts at: 354 1563 1596 3834 4944 5951 354
Size: 1209 33 2238 1110 1007 3315
Fragments arranged by size:
3315 2238 1209 1110 1007 33
HindIII A'AGCT_T
Cuts at: 5029 5029
Size: 8912
KasI G'GCGC_C
Cuts at: 259 376 479 578 754 934 1102 1359 6342
Size: 117 103 99 176 180 168 257 4983
Cuts at: 6342 6590 259
Size: 248 2581
Fragments arranged by size:
4983 2581 257 248 180 176 168 117
103 99
KpnI G_GTAC'C
Cuts at: 5932 5932
Size: 8912
MluI A'CGCG_T
Cuts at: 689 1013 8345 689
Size: 324 7332 1256
Fragments arranged by size:
7332 1256 324
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 929 1010 2354 2628 2813 2936 5411 7583 7767
Size: 81 1344 274 185 123 2475 2172 184
Cuts at: 7767 929
Size: 2074
Fragments arranged by size:
2475 2172 2074 1344 274 185 184 123
81
MscI TGG'CCA
Cuts at: 874 1555 1750 2452 5077 5743 6673 874
Size: 681 195 702 2625 666 930 3113
Fragments arranged by size:
3113 2625 930 702 681 666 195
MslI CAynn'nnrTG
Cuts at: 1516 1543 1636 2026 2321 2440 3134 3388 3943
Size: 27 93 390 295 119 694 254 555
Cuts at: 3943 4627 5005 5737 6901 7028 1516
Size: 684 378 732 1164 127 3400
Fragments arranged by size:
3400 1164 732 694 684 555 390 378
295 254 127 119 93 27
MspA1I CmG'CkG
Cuts at: 142 207 222 369 432 450 477 513 573
Size: 65 15 147 63 18 27 36 60
Cuts at: 573 724 1178 1264 1452 1485 1576 1922 2273
Size: 151 454 86 188 33 91 346 351
Cuts at: 2273 3615 3864 3918 4637 5562 6023 6337 6697
Size: 1342 249 54 719 925 461 314 360
Cuts at: 6697 7710 7955 142
Size: 1013 245 1099
Fragments arranged by size:
1342 1099 1013 925 719 461 454 360
351 346 314 249 245 188 151 147
91 86 65 63 60 54 36 33
27 18 15
NaeI GCC'GGC
Cuts at: 258 1107 2878 3251 4333 7093 258
Size: 849 1771 373 1082 2760 2077
Fragments arranged by size:
2760 2077 1771 1082 849 373
NarI GG'CG_CC
Cuts at: 260 377 480 579 755 935 1103 1360 6343
Size: 117 103 99 176 180 168 257 4983
Cuts at: 6343 6591 260
Size: 248 2581
Fragments arranged by size:
4983 2581 257 248 180 176 168 117
103 99
NcoI C'CATG_G
Cuts at: 125 2675 2774 3092 3129 3428 4847 5744 125
Size: 2550 99 318 37 299 1419 897 3293
Fragments arranged by size:
3293 2550 1419 897 318 299 99 37
NgoMIV G'CCGG_C
Cuts at: 256 1105 2876 3249 4331 7091 256
Size: 849 1771 373 1082 2760 2077
Fragments arranged by size:
2760 2077 1771 1082 849 373
NspI r_CATG'y
Cuts at: 663 1635 2736 3324 4632 5965 6465 7372 663
Size: 972 1101 588 1308 1333 500 907 2203
Fragments arranged by size:
2203 1333 1308 1101 972 907 588 500
PasI CC'CwG_GG
Cuts at: 1251 3843 4266 1251
Size: 2592 423 5897
Fragments arranged by size:
5897 2592 423
PciI A'CATG_T
Cuts at: 7368 7368
Size: 8912
PflMI CCAn_nnn'nTGG
Cuts at: 531 1746 2703 3081 3168 4413 531
Size: 1215 957 378 87 1245 5030
Fragments arranged by size:
5030 1245 1215 957 378 87
PfoI T'CCnGG_A
Cuts at: 2630 4169 4886 5222 5354 5651 2630
Size: 1539 717 336 132 297 5891
Fragments arranged by size:
5891 1539 717 336 297 132
PmeI GTTT'AAAC
Cuts at: 5911 5911
Size: 8912
PmlI CAC'GTG
Cuts at: 2160 2160
Size: 8912
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 114 146 8051 8083 114
Size: 32 7905 32 943
Fragments arranged by size:
7905 943 32 32
PpuMI rG'GwC_Cy
Cuts at: 1256 2070 2843 3165 4989 1256
Size: 814 773 322 1824 5179
Fragments arranged by size:
5179 1824 814 773 322
PshAI GACnn'nnGTC
Cuts at: 2720 4870 2720
Size: 2150 6762
PsiI TTA'TAA
Cuts at: 5759 5759
Size: 8912
PspOMI G'GGCC_C
Cuts at: 411 1911 3096 3242 3509 411
Size: 1500 1185 146 267 5814
Fragments arranged by size:
5814 1500 1185 267 146
PstI C_TGCA'G
Cuts at: 512 1478 1499 1991 2233 2771 3917 3964 4055
Size: 966 21 492 242 538 1146 47 91
Cuts at: 4055 4115 5561 5837 5959 6644 512
Size: 60 1446 276 122 685 2780
Fragments arranged by size:
2780 1446 1146 966 685 538 492 276
242 122 91 60 47 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8912
PvuII CAG'CTG
Cuts at: 369 450 513 573 1452 2273 3864 3918 4637
Size: 81 63 60 879 821 1591 54 719
Cuts at: 4637 5562 6337 6697 369
Size: 925 775 360 2584
Fragments arranged by size:
2584 1591 925 879 821 775 719 360
81 63 60 54
RsrII CG'GwC_CG
Cuts at: 7107 7107
Size: 8912
SacI G_AGCT'C
Cuts at: 3161 5926 3161
Size: 2765 6147
SacII CC_GC'GG
Cuts at: 143 208 478 725 1179 1265 143
Size: 65 270 247 454 86 7790
Fragments arranged by size:
7790 454 270 247 86 65
SalI G'TCGA_C
Cuts at: 4942 5949 4942
Size: 1007 7905
SanDI GG'GwC_CC
Cuts at: 1256 2070 2843 1256
Size: 814 773 7325
Fragments arranged by size:
7325 814 773
SapI GCTCTTCn'nnn_
Cuts at: 886 2572 4223 6935 7145 886
Size: 1686 1651 2712 210 2653
Fragments arranged by size:
2712 2653 1686 1651 210
SbfI CC_TGCA'GG
Cuts at: 5959 5959
Size: 8912
ScaI AGT'ACT
Cuts at: 4498 5341 8429 4498
Size: 843 3088 4981
Fragments arranged by size:
4981 3088 843
SexAI A'CCwGG_T
Cuts at: 2552 2552
Size: 8912
SfcI C'TryA_G
Cuts at: 33 508 1188 1474 1495 1987 2229 2767 3913
Size: 475 680 286 21 492 242 538 1146
Cuts at: 3913 3960 4051 4111 5557 5833 5889 5955 6640
Size: 47 91 60 1446 276 56 66 685
Cuts at: 6640 7633 7824 33
Size: 993 191 1121
Fragments arranged by size:
1446 1146 1121 993 685 680 538 492
475 286 276 242 191 91 66 60
56 47 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 403 403
Size: 8912
SfoI GGC'GCC
Cuts at: 261 378 481 580 756 936 1104 1361 6344
Size: 117 103 99 176 180 168 257 4983
Cuts at: 6344 6592 261
Size: 248 2581
Fragments arranged by size:
4983 2581 257 248 180 176 168 117
103 99
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8912
SgrAI Cr'CCGG_yG
Cuts at: 1105 1105
Size: 8912
SmaI CCC'GGG
Cuts at: 166 1219 1532 3043 3507 3699 5934 166
Size: 1053 313 1511 464 192 2235 3144
Fragments arranged by size:
3144 2235 1511 1053 464 313 192
SmlI C'TyrA_G
Cuts at: 919 1609 4948 6067 6982 7474 7736 919
Size: 690 3339 1119 915 492 262 2095
Fragments arranged by size:
3339 2095 1119 915 690 492 262
SnaBI TAC'GTA
Cuts at: 5904 5904
Size: 8912
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8912
SphI G_CATG'C
Cuts at: 663 1635 5965 6465 663
Size: 972 4330 500 3110
Fragments arranged by size:
4330 3110 972 500
SrfI GCCC'GGGC
Cuts at: 166 166
Size: 8912
StuI AGG'CCT
Cuts at: 460 2350 4894 5527 5899 460
Size: 1890 2544 633 372 3473
Fragments arranged by size:
3473 2544 1890 633 372
StyI C'CwwG_G
Cuts at: 125 938 2675 2774 3092 3101 3129 3428 4136
Size: 813 1737 99 318 9 28 299 708
Cuts at: 4136 4847 5255 5600 5744 6046 6400 125
Size: 711 408 345 144 302 354 2637
Fragments arranged by size:
2637 1737 813 711 708 408 354 345
318 302 299 144 99 28 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1194 1792 2514 3819 4310 5430 6495 6628 7087
Size: 598 722 1305 491 1120 1065 133 459
Cuts at: 7087 7296 1194
Size: 209 2810
Fragments arranged by size:
2810 1305 1120 1065 722 598 491 459
209 133
TatI w'GTAC_w
Cuts at: 4496 4631 5339 8427 4496
Size: 135 708 3088 4981
Fragments arranged by size:
4981 3088 708 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1471 1503 1831 1863 2901 2933 2953 2985 8429
Size: 32 328 32 1038 32 20 32 5444
Cuts at: 8429 8461 1471
Size: 32 1922
Fragments arranged by size:
5444 1922 1038 328 32 32 32 32
32 20
Tth111I GACn'n_nGTC
Cuts at: 6709 6709
Size: 8912
XbaI T'CTAG_A
Cuts at: 5943 5943
Size: 8912
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 387 849 2655 3915 5742 6407 387
Size: 462 1806 1260 1827 665 2892
Fragments arranged by size:
2892 1827 1806 1260 665 462
XmaI C'CCGG_G
Cuts at: 164 1217 1530 3041 3505 3697 5932 164
Size: 1053 313 1511 464 192 2235 3144
Fragments arranged by size:
3144 2235 1511 1053 464 313 192
XmnI GAAnn'nnTTC
Cuts at: 5143 5725 5143
Size: 582 8330
ZraI GAC'GTC
Cuts at: 1736 4941 1736
Size: 3205 5707
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AscI AvrII BfrBI BmtI BsiWI BstZ17I
CspCI EcoRV FseI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PspXI PsrI SspI SwaI XhoI