Restriction map for
pF1KSDA0632
(Circular) (Six-base) MAPSORT of: pF1KSDA0632.seq Check: 4470 from: 1 to: 5239
pF1KSDA0632 5239 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1839 2275 1839
Size: 436 4803
Acc65I G'GTAC_C
Cuts at: 2045 2253 2045
Size: 208 5031
AclI AA'CG_TT
Cuts at: 5079 5079
Size: 5239
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 637 712 841 907 963 1171 1180 1438
Size: 391 75 129 66 56 208 9 258
Cuts at: 1438 3062 3494 4243 4511 246
Size: 1624 432 749 268 974
Fragments arranged by size:
1624 974 749 432 391 268 258 208
129 75 66 56 9
AflIII A'CryG_T
Cuts at: 2589 3695 4672 2589
Size: 1106 977 3156
Fragments arranged by size:
3156 1106 977
AgeI A'CCGG_T
Cuts at: 2042 3627 2042
Size: 1585 3654
AleI CACnn'nnGTG
Cuts at: 1408 1702 1408
Size: 294 4945
AlwNI CAG_nnn'CTG
Cuts at: 615 837 2109 2542 4111 615
Size: 222 1272 433 1569 1743
Fragments arranged by size:
1743 1569 1272 433 222
ApaI G_GGCC'C
Cuts at: 165 165
Size: 5239
ApaLI G'TGCA_C
Cuts at: 4009 4009
Size: 5239
ApoI r'AATT_y
Cuts at: 2241 4592 4848 2241
Size: 2351 256 2632
Fragments arranged by size:
2632 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5239
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5239
AvaI C'yCGr_G
Cuts at: 333 1112 1459 2257 333
Size: 779 347 798 3315
Fragments arranged by size:
3315 798 779 347
AvrII C'CTAG_G
Cuts at: 1125 1125
Size: 5239
BamHI G'GATC_C
Cuts at: 2262 2262
Size: 5239
BanI G'GyrC_C
Cuts at: 255 1817 2045 2253 2667 2915 2950 255
Size: 1562 228 208 414 248 35 2544
Fragments arranged by size:
2544 1562 414 248 228 208 35
BanII G_rGCy'C
Cuts at: 165 328 503 743 860 1385 2251 3281 165
Size: 163 175 240 117 525 866 1030 2123
Fragments arranged by size:
2123 1030 866 525 240 175 163 117
BbeI G_GCGC'C
Cuts at: 259 2671 2919 259
Size: 2412 248 2579
Fragments arranged by size:
2579 2412 248
BbsI GAAGACnn'nnnn_
Cuts at: 374 1021 374
Size: 647 4592
BciVI GTATCCnnnnn_n'
Cuts at: 1362 3135 3898 5239 1362
Size: 1773 763 1341 1362
Fragments arranged by size:
1773 1362 1341 763
BclI T'GATC_A
Cuts at: 563 2757 563
Size: 2194 3045
BglI GCCn_nnn'nGGC
Cuts at: 217 1541 217
Size: 1324 3915
BglII A'GATC_T
Cuts at: 2752 4791 2752
Size: 2039 3200
BlpI GC'TnA_GC
Cuts at: 2349 2349
Size: 5239
Bme1580I G_kGCm'C
Cuts at: 165 959 1346 1902 2214 2862 2955 4013 165
Size: 794 387 556 312 648 93 1058 1391
Fragments arranged by size:
1391 1058 794 648 556 387 312 93
BmrI ACTGGGnnnn_n'
Cuts at: 441 1189 2016 2449 2530 2865 5011 441
Size: 748 827 433 81 335 2146 669
Fragments arranged by size:
2146 827 748 669 433 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 161 3604 161
Size: 3443 1796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 1021 1297 1304 1558 1753 2029 2089 757
Size: 264 276 7 254 195 276 60 3907
Fragments arranged by size:
3907 276 276 264 254 195 60 7
Bpu10I CC'TnA_GC
Cuts at: 1549 1549
Size: 5239
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2413 3292 3786 4084 2413
Size: 879 494 298 3568
Fragments arranged by size:
3568 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 967 1488 1721 4943 967
Size: 521 233 3222 1263
Fragments arranged by size:
3222 1263 521 233
BsaAI yAC'GTr
Cuts at: 960 2229 2590 960
Size: 1269 361 3609
Fragments arranged by size:
3609 1269 361
BsaBI GATnn'nnATC
Cuts at: 6 2751 4790 6
Size: 2745 2039 455
Fragments arranged by size:
2745 2039 455
BsaHI Gr'CG_yC
Cuts at: 256 749 2668 2916 3620 5116 256
Size: 493 1919 248 704 1496 379
Fragments arranged by size:
1919 1496 704 493 379 248
BsaWI w'CCGG_w
Cuts at: 495 1025 1329 2042 2156 2426 2947 3627 3901
Size: 530 304 713 114 270 521 680 274
Cuts at: 3901 4048 4459 495
Size: 147 411 1275
Fragments arranged by size:
1275 713 680 530 521 411 304 274
270 147 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 331 361 397 427 331
Size: 30 36 30 5143
Fragments arranged by size:
5143 36 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 261 264 388 391 394 397 405 637 985
Size: 3 124 3 3 3 8 232 348
Cuts at: 985 1186 261
Size: 201 4314
Fragments arranged by size:
4314 348 232 201 124 8 3 3
3 3
BseYI C'CCAG_C
Cuts at: 1384 1506 1722 2662 3420 3999 4495 1384
Size: 122 216 940 758 579 496 2128
Fragments arranged by size:
2128 940 758 579 496 216 122
BsiEI CG_ry'CG
Cuts at: 104 2779 2825 4035 104
Size: 2675 46 1210 1308
Fragments arranged by size:
2675 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 860 1568 1667 2251 3029 3219 4013 860
Size: 708 99 584 778 190 794 2086
Fragments arranged by size:
2086 794 778 708 584 190 99
Bsp1286I G_dGCh'C
Cuts at: 165 328 503 743 860 959 1346 1385 1568
Size: 163 175 240 117 99 387 39 183
Cuts at: 1568 1667 1902 2214 2251 2862 2955 3029 3219
Size: 99 235 312 37 611 93 74 190
Cuts at: 3219 3281 4013 165
Size: 62 732 1391
Fragments arranged by size:
1391 732 611 387 312 240 235 190
183 175 163 117 99 99 93 74
62 39 37
BspEI T'CCGG_A
Cuts at: 1329 2156 4459 1329
Size: 827 2303 2109
Fragments arranged by size:
2303 2109 827
BspHI T'CATG_A
Cuts at: 4415 4415
Size: 5239
BspMI ACCTGCnnnn'nnnn_
Cuts at: 467 1103 1796 2213 2287 2501 2803 3184 467
Size: 636 693 417 74 214 302 381 2522
Fragments arranged by size:
2522 693 636 417 381 302 214 74
BsrBI CCG'CTC
Cuts at: 50 3529 3583 5069 5234 50
Size: 3479 54 1486 165 55
Fragments arranged by size:
3479 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3149 3149
Size: 5239
BsrFI r'CCGG_y
Cuts at: 1514 1755 2042 2185 3235 3416 3627 1514
Size: 241 287 143 1050 181 211 3126
Fragments arranged by size:
3126 1050 287 241 211 181 143
BssSI C'ACGA_G
Cuts at: 3508 3868 3508
Size: 360 4879
BstAPI GCAn_nnn'nTGC
Cuts at: 837 2614 837
Size: 1777 3462
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5239
BstEII G'GTnAC_C
Cuts at: 1497 1872 1497
Size: 375 4864
BstYI r'GATC_y
Cuts at: 1 2262 2729 2752 3087 3333 4347 4433 4445
Size: 2261 467 23 335 246 1014 86 12
Cuts at: 4445 4791 1
Size: 346 449
Fragments arranged by size:
2261 1014 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 913 913
Size: 5239
BtgI C'CryG_G
Cuts at: 125 170 350 638 1430 125
Size: 45 180 288 792 3934
Fragments arranged by size:
3934 792 288 180 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3187 3368 3531 3187
Size: 181 163 4895
Fragments arranged by size:
4895 181 163
BtsI GCAGTG_nn'
Cuts at: 556 1415 2110 2480 556
Size: 859 695 370 3315
Fragments arranged by size:
3315 859 695 370
DraI TTT'AAA
Cuts at: 2236 2236
Size: 5239
DrdI GACnn_nn'nnGTC
Cuts at: 2943 3803 2943
Size: 860 4379
EaeI y'GGCC_r
Cuts at: 244 291 353 1210 1604 1808 1913 2822 2996
Size: 47 62 857 394 204 105 909 174
Cuts at: 2996 3387 3414 4761 244
Size: 391 27 1347 722
Fragments arranged by size:
1347 909 857 722 394 391 204 174
105 62 47 27
EagI C'GGCC_G
Cuts at: 2822 2822
Size: 5239
EarI CTCTTCn'nnn_
Cuts at: 1708 3260 3470 1708
Size: 1552 210 3477
Fragments arranged by size:
3477 1552 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1990 3445 3757 3903 5049 1990
Size: 1455 312 146 1146 2180
Fragments arranged by size:
2180 1455 1146 312 146
EcoICRI GAG'CTC
Cuts at: 858 2249 858
Size: 1391 3848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 637 712 757 841 907 963 1021 1171
Size: 391 75 45 84 66 56 58 150
Cuts at: 1171 1180 1297 1304 1438 1558 1753 2029 2089
Size: 9 117 7 134 120 195 276 60
Cuts at: 2089 3062 3494 4243 4511 246
Size: 973 432 749 268 974
Fragments arranged by size:
974 973 749 432 391 276 268 195
150 134 120 117 84 75 66 60
58 56 45 9 7
EcoNI CCTnn'n_nnAGG
Cuts at: 292 565 694 292
Size: 273 129 4837
Fragments arranged by size:
4837 273 129
EcoO109I rG'GnC_Cy
Cuts at: 161 395 732 1131 1272 1533 1589 1773 1977
Size: 234 337 399 141 261 56 184 204
Cuts at: 1977 2376 161
Size: 399 3024
Fragments arranged by size:
3024 399 399 337 261 234 204 184
141 56
EcoRI G'AATT_C
Cuts at: 2241 2241
Size: 5239
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 717 1038 3256 717
Size: 321 2218 2700
Fragments arranged by size:
2700 2218 321
FspI TGC'GCA
Cuts at: 906 3018 906
Size: 2112 3127
HaeII r_GCGC'y
Cuts at: 259 818 1750 2671 2919 3943 4922 259
Size: 559 932 921 248 1024 979 576
Fragments arranged by size:
1024 979 932 921 576 559 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 167 199 397 429 1044 1076 3079 3109 3111
Size: 32 198 32 615 32 2003 30 2
Cuts at: 3111 3141 167
Size: 30 2265
Fragments arranged by size:
2265 2003 615 198 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1446 2276 1446
Size: 830 4409
KasI G'GCGC_C
Cuts at: 255 2667 2915 255
Size: 2412 248 2579
Fragments arranged by size:
2579 2412 248
KpnI G_GTAC'C
Cuts at: 2049 2257 2049
Size: 208 5031
MluI A'CGCG_T
Cuts at: 4672 4672
Size: 5239
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 368 3910 4094 368
Size: 3542 184 1513
Fragments arranged by size:
3542 1513 184
MscI TGG'CCA
Cuts at: 1810 2998 1810
Size: 1188 4051
MslI CAynn'nnrTG
Cuts at: 1408 1702 1855 3226 3353 1408
Size: 294 153 1371 127 3294
Fragments arranged by size:
3294 1371 294 153 127
MspA1I CmG'CkG
Cuts at: 282 837 1209 1287 1510 2007 2348 2662 3022
Size: 555 372 78 223 497 341 314 360
Cuts at: 3022 4037 4282 282
Size: 1015 245 1239
Fragments arranged by size:
1239 1015 555 497 372 360 341 314
245 223 78
NaeI GCC'GGC
Cuts at: 3418 3418
Size: 5239
NarI GG'CG_CC
Cuts at: 256 2668 2916 256
Size: 2412 248 2579
Fragments arranged by size:
2579 2412 248
NcoI C'CATG_G
Cuts at: 125 170 638 125
Size: 45 468 4726
Fragments arranged by size:
4726 468 45
NgoMIV G'CCGG_C
Cuts at: 3416 3416
Size: 5239
NruI TCG'CGA
Cuts at: 3600 3600
Size: 5239
NspI r_CATG'y
Cuts at: 1267 2290 2790 3699 1267
Size: 1023 500 909 2807
Fragments arranged by size:
2807 1023 909 500
PasI CC'CwG_GG
Cuts at: 1166 2169 1166
Size: 1003 4236
PciI A'CATG_T
Cuts at: 3695 3695
Size: 5239
PflMI CCAn_nnn'nTGG
Cuts at: 509 509
Size: 5239
PfoI T'CCnGG_A
Cuts at: 418 1621 1655 418
Size: 1203 34 4002
Fragments arranged by size:
4002 1203 34
PmeI GTTT'AAAC
Cuts at: 2236 2236
Size: 5239
PmlI CAC'GTG
Cuts at: 960 960
Size: 5239
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4378 4410 4378
Size: 32 5207
PpuMI rG'GwC_Cy
Cuts at: 395 732 1131 1589 1977 395
Size: 337 399 458 388 3657
Fragments arranged by size:
3657 458 399 388 337
PspOMI G'GGCC_C
Cuts at: 161 161
Size: 5239
PstI C_TGCA'G
Cuts at: 842 1635 1793 2284 2969 842
Size: 793 158 491 685 3112
Fragments arranged by size:
3112 793 685 491 158
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5239
PvuII CAG'CTG
Cuts at: 282 837 1287 1510 2007 2662 3022 282
Size: 555 450 223 497 655 360 2499
Fragments arranged by size:
2499 655 555 497 450 360 223
RsrII CG'GwC_CG
Cuts at: 3432 3432
Size: 5239
SacI G_AGCT'C
Cuts at: 860 2251 860
Size: 1391 3848
SalI G'TCGA_C
Cuts at: 2274 2274
Size: 5239
SanDI GG'GwC_CC
Cuts at: 395 1131 1977 395
Size: 736 846 3657
Fragments arranged by size:
3657 846 736
SapI GCTCTTCn'nnn_
Cuts at: 1708 3260 3470 1708
Size: 1552 210 3477
Fragments arranged by size:
3477 1552 210
SbfI CC_TGCA'GG
Cuts at: 2284 2284
Size: 5239
ScaI AGT'ACT
Cuts at: 4756 4756
Size: 5239
SexAI A'CCwGG_T
Cuts at: 1667 1667
Size: 5239
SfcI C'TryA_G
Cuts at: 33 204 808 838 1069 1475 1631 1789 1840
Size: 171 604 30 231 406 156 158 51
Cuts at: 1840 1893 2280 2965 3960 4151 33
Size: 53 387 685 995 191 1121
Fragments arranged by size:
1121 995 685 604 406 387 231 191
171 158 156 53 51 30
SfoI GGC'GCC
Cuts at: 257 2669 2917 257
Size: 2412 248 2579
Fragments arranged by size:
2579 2412 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5239
SmaI CCC'GGG
Cuts at: 1461 2259 1461
Size: 798 4441
SmlI C'TyrA_G
Cuts at: 2392 3307 3801 4063 2392
Size: 915 494 262 3568
Fragments arranged by size:
3568 915 494 262
SnaBI TAC'GTA
Cuts at: 2229 2229
Size: 5239
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5239
SphI G_CATG'C
Cuts at: 2290 2790 2290
Size: 500 4739
StuI AGG'CCT
Cuts at: 700 700
Size: 5239
StyI C'CwwG_G
Cuts at: 125 170 638 1125 1213 1232 1742 2049 2214
Size: 45 468 487 88 19 510 307 165
Cuts at: 2214 2371 2725 125
Size: 157 354 2639
Fragments arranged by size:
2639 510 487 468 354 307 165 157
88 45 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2820 2953 3412 3623 2820
Size: 133 459 211 4436
Fragments arranged by size:
4436 459 211 133
TatI w'GTAC_w
Cuts at: 4754 4754
Size: 5239
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 331 363 4756 4788 331
Size: 32 4393 32 782
Fragments arranged by size:
4393 782 32 32
Tth111I GACn'n_nGTC
Cuts at: 3034 3034
Size: 5239
XbaI T'CTAG_A
Cuts at: 2268 2268
Size: 5239
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1263 2732 1263
Size: 1469 3770
XmaI C'CCGG_G
Cuts at: 1459 2257 1459
Size: 798 4441
XmnI GAAnn'nnTTC
Cuts at: 700 700
Size: 5239
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
BaeI BbvCI BcgI BfrBI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstXI BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NsiI PacI PshAI PsiI PspXI PsrI
SacII SfiI SgrAI SrfI SspI SwaI XhoI ZraI