Restriction map for
pF1KSDA0638
(Circular) (Six-base) MAPSORT of: pF1KSDA0638.seq Check: 866 from: 1 to: 7270
pF1KSDA0638 7270 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3416 4076 3416
Size: 660 6610
AccI GT'mk_AC
Cuts at: 2888 4306 2888
Size: 1418 5852
Acc65I G'GTAC_C
Cuts at: 978 2903 4284 978
Size: 1925 1381 3964
Fragments arranged by size:
3964 1925 1381
AclI AA'CG_TT
Cuts at: 7110 7110
Size: 7270
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 1710 3310 3850 4039 5093 5525 6274 6542
Size: 1156 1600 540 189 1054 432 749 268
Cuts at: 6542 554
Size: 1282
Fragments arranged by size:
1600 1282 1156 1054 749 540 432 268
189
AfeI AGC'GCT
Cuts at: 2167 3147 3952 2167
Size: 980 805 5485
Fragments arranged by size:
5485 980 805
AflII C'TTAA_G
Cuts at: 1503 1503
Size: 7270
AflIII A'CryG_T
Cuts at: 3533 4620 5726 6703 3533
Size: 1087 1106 977 4100
Fragments arranged by size:
4100 1106 1087 977
AgeI A'CCGG_T
Cuts at: 5658 5658
Size: 7270
AhdI GACnn_n'nnGTC
Cuts at: 3534 3534
Size: 7270
AleI CACnn'nnGTG
Cuts at: 4108 4108
Size: 7270
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2249 2249
Size: 7270
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2536 2568 2536
Size: 32 7238
AlwNI CAG_nnn'CTG
Cuts at: 1138 1231 1291 1965 2295 2346 2613 2763 2913
Size: 93 60 674 330 51 267 150 150
Cuts at: 2913 3663 3735 4231 4573 6142 1138
Size: 750 72 496 342 1569 2266
Fragments arranged by size:
2266 1569 750 674 496 342 330 267
150 150 93 72 60 51
ApaI G_GGCC'C
Cuts at: 358 587 3364 358
Size: 229 2777 4264
Fragments arranged by size:
4264 2777 229
ApaLI G'TGCA_C
Cuts at: 6040 6040
Size: 7270
ApoI r'AATT_y
Cuts at: 4272 6623 6879 4272
Size: 2351 256 4663
Fragments arranged by size:
4663 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7270
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7270
AvaI C'yCGr_G
Cuts at: 2360 2735 3366 4288 2360
Size: 375 631 922 5342
Fragments arranged by size:
5342 922 631 375
BamHI G'GATC_C
Cuts at: 4293 4293
Size: 7270
BanI G'GyrC_C
Cuts at: 400 844 978 1707 1830 2903 4284 4698 4946
Size: 444 134 729 123 1073 1381 414 248
Cuts at: 4946 4981 400
Size: 35 2689
Fragments arranged by size:
2689 1381 1073 729 444 414 248 134
123 35
BanII G_rGCy'C
Cuts at: 358 449 579 587 700 862 1174 1530 1681
Size: 91 130 8 113 162 312 356 151
Cuts at: 1681 1744 1762 2289 2706 3364 3475 4123 4282
Size: 63 18 527 417 658 111 648 159
Cuts at: 4282 5312 358
Size: 1030 2316
Fragments arranged by size:
2316 1030 658 648 527 417 356 312
162 159 151 130 113 111 91 63
18 8
BbeI G_GCGC'C
Cuts at: 848 4702 4950 848
Size: 3854 248 3168
Fragments arranged by size:
3854 3168 248
BbsI GAAGACnn'nnnn_
Cuts at: 2845 3838 3954 2845
Size: 993 116 6161
Fragments arranged by size:
6161 993 116
BbvCI CC'TCA_GC
Cuts at: 3308 4232 3308
Size: 924 6346
BciVI GTATCCnnnnn_n'
Cuts at: 5166 5929 7270 5166
Size: 763 1341 5166
Fragments arranged by size:
5166 1341 763
BclI T'GATC_A
Cuts at: 4788 4788
Size: 7270
BglI GCCn_nnn'nGGC
Cuts at: 468 1472 1621 2365 3368 3417 468
Size: 1004 149 744 1003 49 4321
Fragments arranged by size:
4321 1004 1003 744 149 49
BglII A'GATC_T
Cuts at: 4783 6822 4783
Size: 2039 5231
BlpI GC'TnA_GC
Cuts at: 1063 1738 2630 3106 3284 3886 4380 1063
Size: 675 892 476 178 602 494 3953
Fragments arranged by size:
3953 892 675 602 494 476 178
Bme1580I G_kGCm'C
Cuts at: 358 403 587 971 1659 2303 2385 2418 3364
Size: 45 184 384 688 644 82 33 946
Cuts at: 3364 3723 4893 4986 6044 358
Size: 359 1170 93 1058 1584
Fragments arranged by size:
1584 1170 1058 946 688 644 384 359
184 93 82 45 33
BmrI ACTGGGnnnn_n'
Cuts at: 935 1702 1778 4480 4561 4896 7042 935
Size: 767 76 2702 81 335 2146 1163
Fragments arranged by size:
2702 2146 1163 767 335 81 76
BmtI G_CTAG'C
Cuts at: 2269 2269
Size: 7270
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 894 1656 5635 894
Size: 762 3979 2529
Fragments arranged by size:
3979 2529 762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 353 773 846 866 978 1268 1303 1337
Size: 31 420 73 20 112 290 35 34
Cuts at: 1337 1797 1915 1987 2236 2539 3487 3685 322
Size: 460 118 72 249 303 948 198 3907
Fragments arranged by size:
3907 948 460 420 303 290 249 198
118 112 73 72 35 34 31 20
Bpu10I CC'TnA_GC
Cuts at: 2288 2705 3308 4232 2288
Size: 417 603 924 5326
Fragments arranged by size:
5326 924 603 417
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2170 2299 3958 4444 5323 5817 6115 2170
Size: 129 1659 486 879 494 298 3325
Fragments arranged by size:
3325 1659 879 494 486 298 129
BsaI GGTCTCn'nnnn_
Cuts at: 1963 3208 3481 6974 1963
Size: 1245 273 3493 2259
Fragments arranged by size:
3493 2259 1245 273
BsaAI yAC'GTr
Cuts at: 2304 3879 4260 4621 2304
Size: 1575 381 361 4953
Fragments arranged by size:
4953 1575 381 361
BsaBI GATnn'nnATC
Cuts at: 6 4782 6821 6
Size: 4776 2039 455
Fragments arranged by size:
4776 2039 455
BsaHI Gr'CG_yC
Cuts at: 845 4699 4947 5651 7147 845
Size: 3854 248 704 1496 968
Fragments arranged by size:
3854 1496 968 704 248
BsaWI w'CCGG_w
Cuts at: 2618 3809 4457 4978 5658 5932 6079 6490 2618
Size: 1191 648 521 680 274 147 411 3398
Fragments arranged by size:
3398 1191 680 648 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 760 2137 2206 2599 2602 3063 3066 3081 3084
Size: 1377 69 393 3 461 3 15 3
Cuts at: 3084 3087 3448 3586 3682 3685 760
Size: 3 361 138 96 3 4345
Fragments arranged by size:
4345 1377 461 393 361 138 96 69
15 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 1093 1179 1224 1333 1607 1650 1743 2023 2035
Size: 86 45 109 274 43 93 280 12
Cuts at: 2035 2216 2295 2689 2925 3101 3354 4128 4693
Size: 181 79 394 236 176 253 774 565
Cuts at: 4693 5451 6030 6526 1093
Size: 758 579 496 1837
Fragments arranged by size:
1837 774 758 579 565 496 394 280
274 253 236 181 176 109 93 86
79 45 43 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 417 2217 2734 3859 4097 202
Size: 215 1800 517 1125 238 3375
Fragments arranged by size:
3375 1800 1125 517 238 215
BsiEI CG_ry'CG
Cuts at: 104 3969 4810 4856 6066 104
Size: 3865 841 46 1210 1308
Fragments arranged by size:
3865 1308 1210 841 46
BsiHKAI G_wGCw'C
Cuts at: 377 700 1044 1239 1530 2215 2228 3446 3475
Size: 323 344 195 291 685 13 1218 29
Cuts at: 3475 3887 4282 5060 5250 6044 377
Size: 412 395 778 190 794 1603
Fragments arranged by size:
1603 1218 794 778 685 412 395 344
323 291 195 190 29 13
BsmI GAATG_Cn'
Cuts at: 1256 2751 1256
Size: 1495 5775
BsmBI CGTCTCn'nnnn_
Cuts at: 4003 4003
Size: 7270
Bsp1286I G_dGCh'C
Cuts at: 358 377 403 449 579 587 700 862 971
Size: 19 26 46 130 8 113 162 109
Cuts at: 971 1044 1174 1239 1530 1659 1681 1744 1762
Size: 73 130 65 291 129 22 63 18
Cuts at: 1762 2215 2228 2289 2303 2385 2418 2706 3364
Size: 453 13 61 14 82 33 288 658
Cuts at: 3364 3446 3475 3723 3887 4123 4282 4893 4986
Size: 82 29 248 164 236 159 611 93
Cuts at: 4986 5060 5250 5312 6044 358
Size: 74 190 62 732 1584
Fragments arranged by size:
1584 732 658 611 453 291 288 248
236 190 164 162 159 130 130 129
113 109 93 82 82 74 73 65
63 62 61 46 33 29 26 22
19 18 14 13 8
BspEI T'CCGG_A
Cuts at: 2618 3809 6490 2618
Size: 1191 2681 3398
Fragments arranged by size:
3398 2681 1191
BspHI T'CATG_A
Cuts at: 4250 6446 4250
Size: 2196 5074
BspMI ACCTGCnnnn'nnnn_
Cuts at: 401 722 827 990 2991 3416 3681 3809 3877
Size: 321 105 163 2001 425 265 128 68
Cuts at: 3877 4076 4318 4532 4834 5215 401
Size: 199 242 214 302 381 2456
Fragments arranged by size:
2456 2001 425 381 321 302 265 242
214 199 163 128 105 68
BsrBI CCG'CTC
Cuts at: 50 1365 1848 1923 2283 2379 2412 2568 3011
Size: 1315 483 75 360 96 33 156 443
Cuts at: 3011 3636 3675 5560 5614 7100 7265 50
Size: 625 39 1885 54 1486 165 55
Fragments arranged by size:
1885 1486 1315 625 483 443 360 165
156 96 75 55 54 39 33
BsrDI GCAATG_nn'
Cuts at: 432 5180 432
Size: 4748 2522
BsrFI r'CCGG_y
Cuts at: 1466 1904 2602 3614 3968 4160 5266 5447 5658
Size: 438 698 1012 354 192 1106 181 211
Cuts at: 5658 1466
Size: 3078
Fragments arranged by size:
3078 1106 1012 698 438 354 211 192
181
BssSI C'ACGA_G
Cuts at: 2087 5539 5899 2087
Size: 3452 360 3458
Fragments arranged by size:
3458 3452 360
BstAPI GCAn_nnn'nTGC
Cuts at: 431 491 2346 3882 3899 4645 431
Size: 60 1855 1536 17 746 3056
Fragments arranged by size:
3056 1855 1536 746 60 17
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7270
BstXI CCAn_nnnn'nTGG
Cuts at: 855 855
Size: 7270
BstYI r'GATC_y
Cuts at: 1 1560 2696 4293 4760 4783 5118 5364 6378
Size: 1559 1136 1597 467 23 335 246 1014
Cuts at: 6378 6464 6476 6822 1
Size: 86 12 346 449
Fragments arranged by size:
1597 1559 1136 1014 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2018 2018
Size: 7270
BtgI C'CryG_G
Cuts at: 125 176 1617 3855 3903 125
Size: 51 1441 2238 48 3492
Fragments arranged by size:
3492 2238 1441 51 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2631 5218 5399 5562 2631
Size: 2587 181 163 4339
Fragments arranged by size:
4339 2587 181 163
BtsI GCAGTG_nn'
Cuts at: 270 375 921 1365 2383 2416 2447 2905 3199
Size: 105 546 444 1018 33 31 458 294
Cuts at: 3199 4080 4511 270
Size: 881 431 3029
Fragments arranged by size:
3029 1018 881 546 458 444 431 294
105 33 31
DraI TTT'AAA
Cuts at: 4267 4267
Size: 7270
DraIII CAC_nnn'GTG
Cuts at: 252 943 1696 3882 252
Size: 691 753 2186 3640
Fragments arranged by size:
3640 2186 753 691
DrdI GACnn_nn'nnGTC
Cuts at: 4974 5834 4974
Size: 860 6410
EaeI y'GGCC_r
Cuts at: 1202 2600 2604 2669 2918 4853 5027 5418 5445
Size: 1398 4 65 249 1935 174 391 27
Cuts at: 5445 6792 1202
Size: 1347 1680
Fragments arranged by size:
1935 1680 1398 1347 391 249 174 65
27 4
EagI C'GGCC_G
Cuts at: 4853 4853
Size: 7270
EarI CTCTTCn'nnn_
Cuts at: 906 2273 2964 3090 3257 4081 5291 5501 906
Size: 1367 691 126 167 824 1210 210 2675
Fragments arranged by size:
2675 1367 1210 824 691 210 167 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1162 2127 3693 5476 5788 5934 7080 1162
Size: 965 1566 1783 312 146 1146 1352
Fragments arranged by size:
1783 1566 1352 1146 965 312 146
EcoICRI GAG'CTC
Cuts at: 698 1528 3473 4280 698
Size: 830 1945 807 3688
Fragments arranged by size:
3688 1945 830 807
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 353 554 773 846 866 978 1268 1303
Size: 31 201 219 73 20 112 290 35
Cuts at: 1303 1337 1710 1797 1915 1987 2236 2539 3310
Size: 34 373 87 118 72 249 303 771
Cuts at: 3310 3487 3685 3850 4039 5093 5525 6274 6542
Size: 177 198 165 189 1054 432 749 268
Cuts at: 6542 322
Size: 1050
Fragments arranged by size:
1054 1050 771 749 432 373 303 290
268 249 219 201 198 189 177 165
118 112 87 73 72 35 34 31
20
EcoNI CCTnn'n_nnAGG
Cuts at: 3667 3667
Size: 7270
EcoO109I rG'GnC_Cy
Cuts at: 154 190 354 584 661 711 958 1473 2014
Size: 36 164 230 77 50 247 515 541
Cuts at: 2014 2081 2102 2514 3361 3660 4407 154
Size: 67 21 412 847 299 747 3017
Fragments arranged by size:
3017 847 747 541 515 412 299 247
230 164 77 67 50 36 21
EcoRI G'AATT_C
Cuts at: 4272 4272
Size: 7270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1740 3837 5287 1740
Size: 2097 1450 3723
Fragments arranged by size:
3723 2097 1450
FseI GG_CCGG'CC
Cuts at: 2606 2606
Size: 7270
FspI TGC'GCA
Cuts at: 4072 5049 4072
Size: 977 6293
HaeII r_GCGC'y
Cuts at: 816 848 1608 1917 1965 2169 2667 3149 3225
Size: 32 760 309 48 204 498 482 76
Cuts at: 3225 3954 4702 4950 5974 6953 816
Size: 729 748 248 1024 979 1133
Fragments arranged by size:
1133 1024 979 760 748 729 498 482
309 248 204 76 48 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2688 2720 3520 3552 5110 5140 5142 5172 2688
Size: 32 800 32 1558 30 2 30 4786
Fragments arranged by size:
4786 1558 800 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1246 4307 1246
Size: 3061 4209
HindIII A'AGCT_T
Cuts at: 2984 2984
Size: 7270
KasI G'GCGC_C
Cuts at: 844 4698 4946 844
Size: 3854 248 3168
Fragments arranged by size:
3854 3168 248
KpnI G_GTAC'C
Cuts at: 982 2907 4288 982
Size: 1925 1381 3964
Fragments arranged by size:
3964 1925 1381
MluI A'CGCG_T
Cuts at: 6703 6703
Size: 7270
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2543 5941 6125 2543
Size: 3398 184 3688
Fragments arranged by size:
3688 3398 184
MscI TGG'CCA
Cuts at: 1204 2671 5029 1204
Size: 1467 2358 3445
Fragments arranged by size:
3445 2358 1467
MslI CAynn'nnrTG
Cuts at: 853 2231 3449 4108 5257 5384 853
Size: 1378 1218 659 1149 127 2739
Fragments arranged by size:
2739 1378 1218 1149 659 127
MspA1I CmG'CkG
Cuts at: 300 975 1191 1539 1817 2346 2451 2760 2941
Size: 675 216 348 278 529 105 309 181
Cuts at: 2941 2997 3059 3270 3307 3358 3735 4379 4693
Size: 56 62 211 37 51 377 644 314
Cuts at: 4693 5053 6068 6313 300
Size: 360 1015 245 1257
Fragments arranged by size:
1257 1015 675 644 529 377 360 348
314 309 278 245 216 211 181 105
62 56 51 37
NaeI GCC'GGC
Cuts at: 1468 2604 5449 1468
Size: 1136 2845 3289
Fragments arranged by size:
3289 2845 1136
NarI GG'CG_CC
Cuts at: 845 4699 4947 845
Size: 3854 248 3168
Fragments arranged by size:
3854 3168 248
NcoI C'CATG_G
Cuts at: 125 176 1617 3855 125
Size: 51 1441 2238 3540
Fragments arranged by size:
3540 2238 1441 51
NgoMIV G'CCGG_C
Cuts at: 1466 2602 5447 1466
Size: 1136 2845 3289
Fragments arranged by size:
3289 2845 1136
NheI G'CTAG_C
Cuts at: 2265 2265
Size: 7270
NruI TCG'CGA
Cuts at: 5631 5631
Size: 7270
NspI r_CATG'y
Cuts at: 489 3537 4321 4821 5730 489
Size: 3048 784 500 909 2029
Fragments arranged by size:
3048 2029 909 784 500
PasI CC'CwG_GG
Cuts at: 580 3120 580
Size: 2540 4730
PciI A'CATG_T
Cuts at: 3533 5726 3533
Size: 2193 5077
PflMI CCAn_nnn'nTGG
Cuts at: 177 1231 1398 1965 2562 177
Size: 1054 167 567 597 4885
Fragments arranged by size:
4885 1054 597 567 167
PfoI T'CCnGG_A
Cuts at: 1319 1892 1928 2675 1319
Size: 573 36 747 5914
Fragments arranged by size:
5914 747 573 36
PmeI GTTT'AAAC
Cuts at: 4267 4267
Size: 7270
PmlI CAC'GTG
Cuts at: 2304 3879 2304
Size: 1575 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6409 6441 6409
Size: 32 7238
PpuMI rG'GwC_Cy
Cuts at: 190 661 711 958 2014 190
Size: 471 50 247 1056 5446
Fragments arranged by size:
5446 1056 471 247 50
PspOMI G'GGCC_C
Cuts at: 354 583 3360 354
Size: 229 2777 4264
Fragments arranged by size:
4264 2777 229
PstI C_TGCA'G
Cuts at: 333 609 1134 1442 2351 2393 2426 2456 2946
Size: 276 525 308 909 42 33 30 490
Cuts at: 2946 3002 3206 3239 3710 4191 4315 5000 333
Size: 56 204 33 471 481 124 685 2603
Fragments arranged by size:
2603 909 685 525 490 481 471 308
276 204 124 56 42 33 33 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7270
PvuII CAG'CTG
Cuts at: 1191 2346 2451 2760 2997 3307 3735 4693 5053
Size: 1155 105 309 237 310 428 958 360
Cuts at: 5053 1191
Size: 3408
Fragments arranged by size:
3408 1155 958 428 360 310 309 237
105
RsrII CG'GwC_CG
Cuts at: 5463 5463
Size: 7270
SacI G_AGCT'C
Cuts at: 700 1530 3475 4282 700
Size: 830 1945 807 3688
Fragments arranged by size:
3688 1945 830 807
SalI G'TCGA_C
Cuts at: 4305 4305
Size: 7270
SanDI GG'GwC_CC
Cuts at: 661 661
Size: 7270
SapI GCTCTTCn'nnn_
Cuts at: 2273 3090 3257 5291 5501 2273
Size: 817 167 2034 210 4042
Fragments arranged by size:
4042 2034 817 210 167
SbfI CC_TGCA'GG
Cuts at: 4315 4315
Size: 7270
ScaI AGT'ACT
Cuts at: 6787 6787
Size: 7270
SexAI A'CCwGG_T
Cuts at: 248 248
Size: 7270
SfcI C'TryA_G
Cuts at: 33 329 349 591 605 1012 1130 1438 1749
Size: 296 20 242 14 407 118 308 311
Cuts at: 1749 2347 2389 2422 2452 2885 2942 2998 3202
Size: 598 42 33 30 433 57 56 204
Cuts at: 3202 3235 3706 4187 4311 4996 5991 6182 33
Size: 33 471 481 124 685 995 191 1121
Fragments arranged by size:
1121 995 685 598 481 471 433 407
311 308 296 242 204 191 124 118
57 56 42 33 33 30 20 14
SfiI GGCCn_nnn'nGGCC
Cuts at: 1472 1472
Size: 7270
SfoI GGC'GCC
Cuts at: 846 4700 4948 846
Size: 3854 248 3168
Fragments arranged by size:
3854 3168 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7270
SgrAI Cr'CCGG_yG
Cuts at: 1904 1904
Size: 7270
SmaI CCC'GGG
Cuts at: 2737 4290 2737
Size: 1553 5717
SmlI C'TyrA_G
Cuts at: 1503 2185 2314 3973 4423 5338 5832 6094 1503
Size: 682 129 1659 450 915 494 262 2679
Fragments arranged by size:
2679 1659 915 682 494 450 262 129
SnaBI TAC'GTA
Cuts at: 4260 4260
Size: 7270
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7270
SphI G_CATG'C
Cuts at: 4321 4821 4321
Size: 500 6770
StuI AGG'CCT
Cuts at: 362 1089 2093 2829 362
Size: 727 1004 736 4803
Fragments arranged by size:
4803 1004 736 727
StyI C'CwwG_G
Cuts at: 125 176 194 1617 2471 3855 4402 4756 125
Size: 51 18 1423 854 1384 547 354 2639
Fragments arranged by size:
2639 1423 1384 854 547 354 51 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 954 1171 1684 1701 2155 2230 3172 4851 4984
Size: 217 513 17 454 75 942 1679 133
Cuts at: 4984 5443 5654 954
Size: 459 211 2570
Fragments arranged by size:
2570 1679 942 513 459 454 217 211
133 75 17
TatI w'GTAC_w
Cuts at: 4055 4166 6785 4055
Size: 111 2619 4540
Fragments arranged by size:
4540 2619 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 950 982 1242 1274 1545 1577 3239 3271 6787
Size: 32 260 32 271 32 1662 32 3516
Cuts at: 6787 6819 950
Size: 32 1401
Fragments arranged by size:
3516 1662 1401 271 260 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 5065 5065
Size: 7270
XbaI T'CTAG_A
Cuts at: 4299 4299
Size: 7270
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 154 1002 2058 4763 154
Size: 848 1056 2705 2661
Fragments arranged by size:
2705 2661 1056 848
XmaI C'CCGG_G
Cuts at: 2735 4288 2735
Size: 1553 5717
XmnI GAAnn'nnTTC
Cuts at: 1410 1410
Size: 7270
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NruI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AscI AvrII BaeI BcgI BfrBI BmgBI BsaXI
BsiWI BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoRV
FspAI HpaI MfeI NdeI NotI NsiI PacI PshAI
PsiI PspXI PsrI SacII SrfI SspI SwaI XhoI
ZraI