Restriction map for
pF1KSDA0640
(Circular) (Six-base) MAPSORT of: pF1KSDA0640.seq Check: 5451 from: 1 to: 4894
pF1KSDA0640 4894 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1930 1930
Size: 4894
Acc65I G'GTAC_C
Cuts at: 1908 1908
Size: 4894
AclI AA'CG_TT
Cuts at: 4734 4734
Size: 4894
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 276 886 974 1240 1258 1456 1618 2717
Size: 2 610 88 266 18 198 162 1099
Cuts at: 2717 3149 3898 4166 274
Size: 432 749 268 1002
Fragments arranged by size:
1099 1002 749 610 432 268 266 198
162 88 18 2
AflII C'TTAA_G
Cuts at: 553 553
Size: 4894
AflIII A'CryG_T
Cuts at: 1397 2244 3350 4327 1397
Size: 847 1106 977 1964
Fragments arranged by size:
1964 1106 977 847
AgeI A'CCGG_T
Cuts at: 3282 3282
Size: 4894
AlwNI CAG_nnn'CTG
Cuts at: 1188 1611 2197 3766 1188
Size: 423 586 1569 2316
Fragments arranged by size:
2316 1569 586 423
ApaLI G'TGCA_C
Cuts at: 243 3664 243
Size: 3421 1473
ApoI r'AATT_y
Cuts at: 391 1896 4247 4503 391
Size: 1505 2351 256 782
Fragments arranged by size:
2351 1505 782 256
AseI AT'TA_AT
Cuts at: 20 520 725 1756 20
Size: 500 205 1031 3158
Fragments arranged by size:
3158 1031 500 205
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4894
AvaI C'yCGr_G
Cuts at: 1912 1912
Size: 4894
BamHI G'GATC_C
Cuts at: 1917 1917
Size: 4894
BanI G'GyrC_C
Cuts at: 1908 2322 2570 2605 1908
Size: 414 248 35 4197
Fragments arranged by size:
4197 414 248 35
BanII G_rGCy'C
Cuts at: 1906 2936 1906
Size: 1030 3864
BbeI G_GCGC'C
Cuts at: 2326 2574 2326
Size: 248 4646
BbsI GAAGACnn'nnnn_
Cuts at: 1400 1490 1400
Size: 90 4804
BciVI GTATCCnnnnn_n'
Cuts at: 524 2790 3553 4894 524
Size: 2266 763 1341 524
Fragments arranged by size:
2266 1341 763 524
BclI T'GATC_A
Cuts at: 1796 2412 1796
Size: 616 4278
BfrBI ATG'CAT
Cuts at: 469 469
Size: 4894
BglII A'GATC_T
Cuts at: 2407 4446 2407
Size: 2039 2855
BlpI GC'TnA_GC
Cuts at: 2004 2004
Size: 4894
Bme1580I G_kGCm'C
Cuts at: 247 2517 2610 3668 247
Size: 2270 93 1058 1473
Fragments arranged by size:
2270 1473 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 265 1815 2104 2185 2520 4666 265
Size: 1550 289 81 335 2146 493
Fragments arranged by size:
2146 1550 493 335 289 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 904 3259 904
Size: 2355 2539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 1156 1210 1231 1654 1717 1141
Size: 15 54 21 423 63 4318
Fragments arranged by size:
4318 423 63 54 21 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 1247 1663 1687 2068 2947 3441 3739 205
Size: 1042 416 24 381 879 494 298 1360
Fragments arranged by size:
1360 1042 879 494 416 381 298 24
BsaI GGTCTCn'nnnn_
Cuts at: 210 4598 210
Size: 4388 506
BsaAI yAC'GTr
Cuts at: 1884 2245 1884
Size: 361 4533
BsaBI GATnn'nnATC
Cuts at: 6 2406 4445 6
Size: 2400 2039 455
Fragments arranged by size:
2400 2039 455
BsaHI Gr'CG_yC
Cuts at: 2323 2571 3275 4771 2323
Size: 248 704 1496 2446
Fragments arranged by size:
2446 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1103 2081 2602 3282 3556 3703 4114 1103
Size: 978 521 680 274 147 411 1883
Fragments arranged by size:
1883 978 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1480 1510 1630 1660 1480
Size: 30 120 30 4714
Fragments arranged by size:
4714 120 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 593 1651 157
Size: 436 1058 3400
Fragments arranged by size:
3400 1058 436
BseYI C'CCAG_C
Cuts at: 215 1053 2317 3075 3654 4150 215
Size: 838 1264 758 579 496 959
Fragments arranged by size:
1264 959 838 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1250 1250
Size: 4894
BsiEI CG_ry'CG
Cuts at: 104 2434 2480 3690 104
Size: 2330 46 1210 1308
Fragments arranged by size:
2330 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 247 290 1906 2684 2874 3668 247
Size: 43 1616 778 190 794 1473
Fragments arranged by size:
1616 1473 794 778 190 43
Bsp1286I G_dGCh'C
Cuts at: 247 290 1906 2517 2610 2684 2874 2936 3668
Size: 43 1616 611 93 74 190 62 732
Cuts at: 3668 247
Size: 1473
Fragments arranged by size:
1616 1473 732 611 190 93 74 62
43
BspEI T'CCGG_A
Cuts at: 1103 4114 1103
Size: 3011 1883
BspHI T'CATG_A
Cuts at: 264 1746 4070 264
Size: 1482 2324 1088
Fragments arranged by size:
2324 1482 1088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 557 1821 1942 2156 2458 2839 557
Size: 1264 121 214 302 381 2612
Fragments arranged by size:
2612 1264 381 302 214 121
BsrBI CCG'CTC
Cuts at: 50 3184 3238 4724 4889 50
Size: 3134 54 1486 165 55
Fragments arranged by size:
3134 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2804 2804
Size: 4894
BsrFI r'CCGG_y
Cuts at: 701 2890 3071 3282 701
Size: 2189 181 211 2313
Fragments arranged by size:
2313 2189 211 181
BssSI C'ACGA_G
Cuts at: 1226 3163 3523 1226
Size: 1937 360 2597
Fragments arranged by size:
2597 1937 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1228 2269 1228
Size: 1041 3853
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4894
BstXI CCAn_nnnn'nTGG
Cuts at: 1485 1485
Size: 4894
BstYI r'GATC_y
Cuts at: 1 861 1917 2384 2407 2742 2988 4002 4088
Size: 860 1056 467 23 335 246 1014 86
Cuts at: 4088 4100 4446 1
Size: 12 346 449
Fragments arranged by size:
1056 1014 860 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 1622 1874 125
Size: 1497 252 3145
Fragments arranged by size:
3145 1497 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2842 3023 3186 2842
Size: 181 163 4550
Fragments arranged by size:
4550 181 163
BtsI GCAGTG_nn'
Cuts at: 2135 2135
Size: 4894
DraI TTT'AAA
Cuts at: 344 474 1891 344
Size: 130 1417 3347
Fragments arranged by size:
3347 1417 130
DrdI GACnn_nn'nnGTC
Cuts at: 2598 3458 2598
Size: 860 4034
EaeI y'GGCC_r
Cuts at: 2477 2651 3042 3069 4416 2477
Size: 174 391 27 1347 2955
Fragments arranged by size:
2955 1347 391 174 27
EagI C'GGCC_G
Cuts at: 2477 2477
Size: 4894
EarI CTCTTCn'nnn_
Cuts at: 278 530 1232 1448 1487 1835 2915 3125 278
Size: 252 702 216 39 348 1080 210 2047
Fragments arranged by size:
2047 1080 702 348 252 216 210 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1579 3100 3412 3558 4704 1579
Size: 1521 312 146 1146 1769
Fragments arranged by size:
1769 1521 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1904 1904
Size: 4894
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 276 886 974 1141 1156 1210 1231 1240
Size: 2 610 88 167 15 54 21 9
Cuts at: 1240 1258 1456 1618 1654 1717 2717 3149 3898
Size: 18 198 162 36 63 1000 432 749
Cuts at: 3898 4166 274
Size: 268 1002
Fragments arranged by size:
1002 1000 749 610 432 268 198 167
162 88 63 54 36 21 18 15
9 2
EcoNI CCTnn'n_nnAGG
Cuts at: 1615 1615
Size: 4894
EcoO109I rG'GnC_Cy
Cuts at: 225 1608 1810 2031 225
Size: 1383 202 221 3088
Fragments arranged by size:
3088 1383 221 202
EcoRI G'AATT_C
Cuts at: 391 1896 391
Size: 1505 3389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1288 1545 2911 1288
Size: 257 1366 3271
Fragments arranged by size:
3271 1366 257
FspI TGC'GCA
Cuts at: 2673 2673
Size: 4894
HaeII r_GCGC'y
Cuts at: 2326 2574 3598 4577 2326
Size: 248 1024 979 2643
Fragments arranged by size:
2643 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 846 878 1480 1512 2734 2764 2766 2796 846
Size: 32 602 32 1222 30 2 30 2944
Fragments arranged by size:
2944 1222 602 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1931 1931
Size: 4894
HindIII A'AGCT_T
Cuts at: 559 1309 1468 559
Size: 750 159 3985
Fragments arranged by size:
3985 750 159
KasI G'GCGC_C
Cuts at: 2322 2570 2322
Size: 248 4646
KpnI G_GTAC'C
Cuts at: 1912 1912
Size: 4894
MluI A'CGCG_T
Cuts at: 4327 4327
Size: 4894
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 344 388 1072 1466 1559 3565 3749 344
Size: 44 684 394 93 2006 184 1489
Fragments arranged by size:
2006 1489 684 394 184 93 44
MscI TGG'CCA
Cuts at: 2653 2653
Size: 4894
MslI CAynn'nnrTG
Cuts at: 2881 3008 2881
Size: 127 4767
MspA1I CmG'CkG
Cuts at: 197 1116 1632 2003 2317 2677 3692 3937 197
Size: 919 516 371 314 360 1015 245 1154
Fragments arranged by size:
1154 1015 919 516 371 360 314 245
NaeI GCC'GGC
Cuts at: 3073 3073
Size: 4894
NarI GG'CG_CC
Cuts at: 2323 2571 2323
Size: 248 4646
NcoI C'CATG_G
Cuts at: 125 1622 125
Size: 1497 3397
NgoMIV G'CCGG_C
Cuts at: 3071 3071
Size: 4894
NruI TCG'CGA
Cuts at: 3255 3255
Size: 4894
NsiI A_TGCA'T
Cuts at: 471 471
Size: 4894
NspI r_CATG'y
Cuts at: 336 1401 1945 2445 3354 336
Size: 1065 544 500 909 1876
Fragments arranged by size:
1876 1065 909 544 500
PciI A'CATG_T
Cuts at: 1397 3350 1397
Size: 1953 2941
PflMI CCAn_nnn'nTGG
Cuts at: 1724 1724
Size: 4894
PmeI GTTT'AAAC
Cuts at: 1891 1891
Size: 4894
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4033 4065 4033
Size: 32 4862
PpuMI rG'GwC_Cy
Cuts at: 225 1810 225
Size: 1585 3309
PsiI TTA'TAA
Cuts at: 611 611
Size: 4894
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1355 1387 1355
Size: 32 4862
PstI C_TGCA'G
Cuts at: 1415 1616 1818 1939 2624 1415
Size: 201 202 121 685 3685
Fragments arranged by size:
3685 685 202 201 121
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4894
PvuII CAG'CTG
Cuts at: 1116 1632 2317 2677 1116
Size: 516 685 360 3333
Fragments arranged by size:
3333 685 516 360
RsrII CG'GwC_CG
Cuts at: 3087 3087
Size: 4894
SacI G_AGCT'C
Cuts at: 1906 1906
Size: 4894
SalI G'TCGA_C
Cuts at: 1929 1929
Size: 4894
SapI GCTCTTCn'nnn_
Cuts at: 278 2915 3125 278
Size: 2637 210 2047
Fragments arranged by size:
2637 2047 210
SbfI CC_TGCA'GG
Cuts at: 1616 1939 1616
Size: 323 4571
ScaI AGT'ACT
Cuts at: 4411 4411
Size: 4894
SexAI A'CCwGG_T
Cuts at: 1773 1773
Size: 4894
SfcI C'TryA_G
Cuts at: 33 1411 1612 1814 1935 2620 3615 3806 33
Size: 1378 201 202 121 685 995 191 1121
Fragments arranged by size:
1378 1121 995 685 202 201 191 121
SfoI GGC'GCC
Cuts at: 2324 2572 2324
Size: 248 4646
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4894
SmaI CCC'GGG
Cuts at: 1914 1914
Size: 4894
SmlI C'TyrA_G
Cuts at: 220 553 1262 1642 1666 2047 2962 3456 3718
Size: 333 709 380 24 381 915 494 262
Cuts at: 3718 220
Size: 1396
Fragments arranged by size:
1396 915 709 494 381 380 333 262
24
SnaBI TAC'GTA
Cuts at: 1884 1884
Size: 4894
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4894
SphI G_CATG'C
Cuts at: 1945 2445 1945
Size: 500 4394
StyI C'CwwG_G
Cuts at: 125 1622 2026 2380 125
Size: 1497 404 354 2639
Fragments arranged by size:
2639 1497 404 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2475 2608 3067 3278 2475
Size: 133 459 211 4091
Fragments arranged by size:
4091 459 211 133
TatI w'GTAC_w
Cuts at: 822 4409 822
Size: 3587 1307
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 402 426 434 458 4411 4443 402
Size: 24 8 24 3953 32 853
Fragments arranged by size:
3953 853 32 24 24 8
Tth111I GACn'n_nGTC
Cuts at: 711 2689 711
Size: 1978 2916
XbaI T'CTAG_A
Cuts at: 1923 1923
Size: 4894
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1629 2387 1629
Size: 758 4136
XmaI C'CCGG_G
Cuts at: 1912 1912
Size: 4894
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NsiI NspI PciI PflMI PmeI
PpiI PpuMI PsiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI PacI PasI PfoI PmlI
PshAI PspOMI PspXI SacII SanDI SfiI SgrAI SrfI
SspI StuI SwaI XhoI XmnI ZraI