Restriction map for
pF1KSDA0642
(Circular) (Six-base) MAPSORT of: pF1KSDA0642.seq Check: 1876 from: 1 to: 7097
pF1KSDA0642 7097 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2069 2069
Size: 7097
AatII G_ACGT'C
Cuts at: 948 948
Size: 7097
AccI GT'mk_AC
Cuts at: 1440 4135 1440
Size: 2695 4402
Acc65I G'GTAC_C
Cuts at: 369 3178 4113 369
Size: 2809 935 3353
Fragments arranged by size:
3353 2809 935
AclI AA'CG_TT
Cuts at: 6937 6937
Size: 7097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 1091 1322 1433 1496 2254 2350 3097 3158
Size: 804 231 111 63 758 96 747 61
Cuts at: 3158 3574 4922 5354 6101 6369 287
Size: 416 1348 432 747 268 1015
Fragments arranged by size:
1348 1015 804 758 747 747 432 416
268 231 111 96 63 61
AflIII A'CryG_T
Cuts at: 4449 5553 6530 4449
Size: 1104 977 5016
Fragments arranged by size:
5016 1104 977
AgeI A'CCGG_T
Cuts at: 5485 5485
Size: 7097
AhdI GACnn_n'nnGTC
Cuts at: 1347 1347
Size: 7097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 604 2609 604
Size: 2005 5092
AlwNI CAG_nnn'CTG
Cuts at: 147 1497 1633 2061 2251 2350 3813 4402 5969
Size: 1350 136 428 190 99 1463 589 1567
Cuts at: 5969 147
Size: 1275
Fragments arranged by size:
1567 1463 1350 1275 589 428 190 136
99
ApaI G_GGCC'C
Cuts at: 2742 2742
Size: 7097
ApaLI G'TGCA_C
Cuts at: 1703 5867 1703
Size: 4164 2933
ApoI r'AATT_y
Cuts at: 322 732 775 1820 2489 3315 3456 4101 6450
Size: 410 43 1045 669 826 141 645 2349
Cuts at: 6450 6706 322
Size: 256 713
Fragments arranged by size:
2349 1045 826 713 669 645 410 256
141 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 7097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7097
AvaI C'yCGr_G
Cuts at: 2741 4117 2741
Size: 1376 5721
AvrII C'CTAG_G
Cuts at: 3451 3451
Size: 7097
BamHI G'GATC_C
Cuts at: 4122 4122
Size: 7097
BanI G'GyrC_C
Cuts at: 369 3083 3178 4113 4527 4775 4810 369
Size: 2714 95 935 414 248 35 2656
Fragments arranged by size:
2714 2656 935 414 248 95 35
BanII G_rGCy'C
Cuts at: 177 474 2742 3276 3331 4111 5141 177
Size: 297 2268 534 55 780 1030 2133
Fragments arranged by size:
2268 2133 1030 780 534 297 55
BbeI G_GCGC'C
Cuts at: 3087 4531 4779 3087
Size: 1444 248 5405
Fragments arranged by size:
5405 1444 248
BbsI GAAGACnn'nnnn_
Cuts at: 2126 2481 2658 2126
Size: 355 177 6565
Fragments arranged by size:
6565 355 177
BciVI GTATCCnnnnn_n'
Cuts at: 4995 5756 7097 4995
Size: 761 1341 4995
Fragments arranged by size:
4995 1341 761
BclI T'GATC_A
Cuts at: 2202 4617 2202
Size: 2415 4682
BfrBI ATG'CAT
Cuts at: 591 1776 4036 591
Size: 1185 2260 3652
Fragments arranged by size:
3652 2260 1185
BglI GCCn_nnn'nGGC
Cuts at: 165 165
Size: 7097
BglII A'GATC_T
Cuts at: 4612 6649 4612
Size: 2037 5060
BlpI GC'TnA_GC
Cuts at: 4209 4209
Size: 7097
Bme1580I G_kGCm'C
Cuts at: 1457 1707 2742 4722 4815 5871 1457
Size: 250 1035 1980 93 1056 2683
Fragments arranged by size:
2683 1980 1056 1035 250 93
BmrI ACTGGGnnnn_n'
Cuts at: 4004 4309 4390 4725 6869 4004
Size: 305 81 335 2144 4232
Fragments arranged by size:
4232 2144 335 305 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1209 5464 1209
Size: 4255 2842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 322 1121 1952 2869 3217 200
Size: 122 799 831 917 348 4080
Fragments arranged by size:
4080 917 831 799 348 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3784 4273 5152 5644 5942 3784
Size: 489 879 492 298 4939
Fragments arranged by size:
4939 879 492 489 298
BsaI GGTCTCn'nnnn_
Cuts at: 720 1654 6801 720
Size: 934 5147 1016
Fragments arranged by size:
5147 1016 934
BsaAI yAC'GTr
Cuts at: 4089 4450 4089
Size: 361 6736
BsaBI GATnn'nnATC
Cuts at: 6 1725 4611 6648 6
Size: 1719 2886 2037 455
Fragments arranged by size:
2886 2037 1719 455
BsaHI Gr'CG_yC
Cuts at: 945 3084 4528 4776 5478 6974 945
Size: 2139 1444 248 702 1496 1068
Fragments arranged by size:
2139 1496 1444 1068 702 248
BsaWI w'CCGG_w
Cuts at: 2906 4286 4807 5485 5759 5906 6317 2906
Size: 1380 521 678 274 147 411 3686
Fragments arranged by size:
3686 1380 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 189 219 446 476 1509 1539 3330 3360 189
Size: 30 227 30 1033 30 1791 30 3926
Fragments arranged by size:
3926 1791 1033 227 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 197 461 464 467 587 1201 1432 1517 1976
Size: 264 3 3 120 614 231 85 459
Cuts at: 1976 2679 2737 2788 2812 197
Size: 703 58 51 24 4482
Fragments arranged by size:
4482 703 614 459 264 231 120 85
58 51 24 3 3
BseYI C'CCAG_C
Cuts at: 605 702 1456 2138 2759 4522 5280 5857 6353
Size: 97 754 682 621 1763 758 577 496
Cuts at: 6353 605
Size: 1349
Fragments arranged by size:
1763 1349 758 754 682 621 577 496
97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3283 3982 3283
Size: 699 6398
BsiEI CG_ry'CG
Cuts at: 104 4639 4685 5893 104
Size: 4535 46 1208 1308
Fragments arranged by size:
4535 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 177 1195 1605 1707 1745 3276 4111 4889 5079
Size: 1018 410 102 38 1531 835 778 190
Cuts at: 5079 5871 177
Size: 792 1403
Fragments arranged by size:
1531 1403 1018 835 792 778 410 190
102 38
BsmI GAATG_Cn'
Cuts at: 1105 3288 1105
Size: 2183 4914
BsmBI CGTCTCn'nnnn_
Cuts at: 4063 4063
Size: 7097
Bsp1286I G_dGCh'C
Cuts at: 177 474 1195 1457 1605 1707 1745 2742 3276
Size: 297 721 262 148 102 38 997 534
Cuts at: 3276 3331 4111 4722 4815 4889 5079 5141 5871
Size: 55 780 611 93 74 190 62 730
Cuts at: 5871 177
Size: 1403
Fragments arranged by size:
1403 997 780 730 721 611 534 297
262 190 148 102 93 74 62 55
38
BspEI T'CCGG_A
Cuts at: 2906 6317 2906
Size: 3411 3686
BspHI T'CATG_A
Cuts at: 1937 6273 1937
Size: 4336 2761
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2069 3305 4147 4361 4663 5044 2069
Size: 1236 842 214 302 381 4122
Fragments arranged by size:
4122 1236 842 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5389 5443 6927 7092 50
Size: 5339 54 1484 165 55
Fragments arranged by size:
5339 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 220 1790 5009 220
Size: 1570 3219 2308
Fragments arranged by size:
3219 2308 1570
BsrFI r'CCGG_y
Cuts at: 2780 3515 5095 5276 5485 2780
Size: 735 1580 181 209 4392
Fragments arranged by size:
4392 1580 735 209 181
BssSI C'ACGA_G
Cuts at: 5368 5726 5368
Size: 358 6739
BstAPI GCAn_nnn'nTGC
Cuts at: 2061 3813 4474 2061
Size: 1752 661 4684
Fragments arranged by size:
4684 1752 661
BstBI TT'CG_AA
Cuts at: 110 5458 110
Size: 5348 1749
BstEII G'GTnAC_C
Cuts at: 991 991
Size: 7097
BstXI CCAn_nnnn'nTGG
Cuts at: 1927 1927
Size: 7097
BstYI r'GATC_y
Cuts at: 1 1806 2319 3200 4005 4122 4589 4612 4947
Size: 1805 513 881 805 117 467 23 335
Cuts at: 4947 5193 6205 6291 6303 6649 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1805 1012 881 805 513 467 449 346
335 246 117 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1811 1811
Size: 7097
BtgI C'CryG_G
Cuts at: 125 125
Size: 7097
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5047 5228 5391 5047
Size: 181 163 6753
Fragments arranged by size:
6753 181 163
BtsI GCAGTG_nn'
Cuts at: 1020 1623 3440 3604 4340 1020
Size: 603 1817 164 736 3777
Fragments arranged by size:
3777 1817 736 603 164
DraI TTT'AAA
Cuts at: 4096 4096
Size: 7097
DrdI GACnn_nn'nnGTC
Cuts at: 896 4803 5661 896
Size: 3907 858 2332
Fragments arranged by size:
3907 2332 858
EaeI y'GGCC_r
Cuts at: 1576 4682 4856 5247 5274 6619 1576
Size: 3106 174 391 27 1345 2054
Fragments arranged by size:
3106 2054 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4682 4682
Size: 7097
EarI CTCTTCn'nnn_
Cuts at: 1142 1214 1409 1535 1891 2406 2435 2441 2555
Size: 72 195 126 356 515 29 6 114
Cuts at: 2555 2609 2654 2664 2669 2714 2789 2830 3068
Size: 54 45 10 5 45 75 41 238
Cuts at: 3068 3116 3197 3206 5120 5330 1142
Size: 48 81 9 1914 210 2909
Fragments arranged by size:
2909 1914 515 356 238 210 195 126
114 81 75 72 54 48 45 45
41 29 10 9 6 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2703 5305 5615 5761 6907 2703
Size: 2602 310 146 1146 2893
Fragments arranged by size:
2893 2602 1146 310 146
EcoICRI GAG'CTC
Cuts at: 175 3274 4109 175
Size: 3099 835 3163
Fragments arranged by size:
3163 3099 835
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 287 322 1091 1121 1322 1433 1496 1952
Size: 87 35 769 30 201 111 63 456
Cuts at: 1952 2254 2350 2869 3097 3158 3217 3574 4922
Size: 302 96 519 228 61 59 357 1348
Cuts at: 4922 5354 6101 6369 200
Size: 432 747 268 928
Fragments arranged by size:
1348 928 769 747 519 456 432 357
302 268 228 201 111 96 87 63
61 59 35 30
EcoNI CCTnn'n_nnAGG
Cuts at: 340 340
Size: 7097
EcoO109I rG'GnC_Cy
Cuts at: 1828 2347 3448 3713 4236 1828
Size: 519 1101 265 523 4689
Fragments arranged by size:
4689 1101 523 519 265
EcoRI G'AATT_C
Cuts at: 3315 3456 4101 3315
Size: 141 645 6311
Fragments arranged by size:
6311 645 141
EcoRV GAT'ATC
Cuts at: 1935 1935
Size: 7097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2557 2662 5116 2557
Size: 105 2454 4538
Fragments arranged by size:
4538 2454 105
FspI TGC'GCA
Cuts at: 4878 4878
Size: 7097
HaeII r_GCGC'y
Cuts at: 3087 3771 4531 4779 5801 6780 3087
Size: 684 760 248 1022 979 3404
Fragments arranged by size:
3404 1022 979 760 684 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1333 1365 1507 1539 2239 2271 3093 3125 4013
Size: 32 142 32 700 32 822 32 888
Cuts at: 4013 4045 4939 4969 4971 5001 1333
Size: 32 894 30 2 30 3429
Fragments arranged by size:
3429 894 888 822 700 142 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 4136 4136
Size: 7097
HindIII A'AGCT_T
Cuts at: 1914 2953 1914
Size: 1039 6058
KasI G'GCGC_C
Cuts at: 3083 4527 4775 3083
Size: 1444 248 5405
Fragments arranged by size:
5405 1444 248
KpnI G_GTAC'C
Cuts at: 373 3182 4117 373
Size: 2809 935 3353
Fragments arranged by size:
3353 2809 935
MluI A'CGCG_T
Cuts at: 6530 6530
Size: 7097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 928 1944 3317 3434 4055 5768 5952 928
Size: 1016 1373 117 621 1713 184 2073
Fragments arranged by size:
2073 1713 1373 1016 621 184 117
MscI TGG'CCA
Cuts at: 4858 4858
Size: 7097
MslI CAynn'nnrTG
Cuts at: 1942 5086 5213 1942
Size: 3144 127 3826
Fragments arranged by size:
3826 3144 127
MspA1I CmG'CkG
Cuts at: 133 2580 2859 2940 3813 4208 4522 4882 5895
Size: 2447 279 81 873 395 314 360 1013
Cuts at: 5895 6140 133
Size: 245 1090
Fragments arranged by size:
2447 1090 1013 873 395 360 314 279
245 81
NaeI GCC'GGC
Cuts at: 5278 5278
Size: 7097
NarI GG'CG_CC
Cuts at: 3084 4528 4776 3084
Size: 1444 248 5405
Fragments arranged by size:
5405 1444 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 7097
NdeI CA'TA_TG
Cuts at: 1991 1991
Size: 7097
NgoMIV G'CCGG_C
Cuts at: 5276 5276
Size: 7097
NsiI A_TGCA'T
Cuts at: 593 1778 4038 593
Size: 1185 2260 3652
Fragments arranged by size:
3652 2260 1185
NspI r_CATG'y
Cuts at: 684 3002 4150 4650 5557 684
Size: 2318 1148 500 907 2224
Fragments arranged by size:
2318 2224 1148 907 500
PciI A'CATG_T
Cuts at: 5553 5553
Size: 7097
PflMI CCAn_nnn'nTGG
Cuts at: 2251 2251
Size: 7097
PfoI T'CCnGG_A
Cuts at: 338 338
Size: 7097
PmeI GTTT'AAAC
Cuts at: 4096 4096
Size: 7097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6236 6268 6236
Size: 32 7065
PpuMI rG'GwC_Cy
Cuts at: 1828 3448 1828
Size: 1620 5477
PspOMI G'GGCC_C
Cuts at: 2738 2738
Size: 7097
PstI C_TGCA'G
Cuts at: 921 2066 4144 4829 921
Size: 1145 2078 685 3189
Fragments arranged by size:
3189 2078 1145 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7097
PvuII CAG'CTG
Cuts at: 2940 3813 4522 4882 2940
Size: 873 709 360 5155
Fragments arranged by size:
5155 873 709 360
RsrII CG'GwC_CG
Cuts at: 5292 5292
Size: 7097
SacI G_AGCT'C
Cuts at: 177 3276 4111 177
Size: 3099 835 3163
Fragments arranged by size:
3163 3099 835
SalI G'TCGA_C
Cuts at: 4134 4134
Size: 7097
SapI GCTCTTCn'nnn_
Cuts at: 1142 2441 3068 3116 5120 5330 1142
Size: 1299 627 48 2004 210 2909
Fragments arranged by size:
2909 2004 1299 627 210 48
SbfI CC_TGCA'GG
Cuts at: 4144 4144
Size: 7097
ScaI AGT'ACT
Cuts at: 6614 6614
Size: 7097
SexAI A'CCwGG_T
Cuts at: 1326 1326
Size: 7097
SfcI C'TryA_G
Cuts at: 33 917 2062 2291 3500 4140 4825 5818 6009
Size: 884 1145 229 1209 640 685 993 191
Cuts at: 6009 33
Size: 1121
Fragments arranged by size:
1209 1145 1121 993 884 685 640 229
191
SfoI GGC'GCC
Cuts at: 3085 4529 4777 3085
Size: 1444 248 5405
Fragments arranged by size:
5405 1444 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7097
SmaI CCC'GGG
Cuts at: 2743 4119 2743
Size: 1376 5721
SmlI C'TyrA_G
Cuts at: 3763 4252 5167 5659 5921 3763
Size: 489 915 492 262 4939
Fragments arranged by size:
4939 915 492 489 262
SnaBI TAC'GTA
Cuts at: 4089 4089
Size: 7097
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7097
SphI G_CATG'C
Cuts at: 4150 4650 4150
Size: 500 6597
StyI C'CwwG_G
Cuts at: 125 720 3451 3740 4231 4585 125
Size: 595 2731 289 491 354 2637
Fragments arranged by size:
2731 2637 595 491 354 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4048 4680 4813 5272 5481 4048
Size: 632 133 459 209 5664
Fragments arranged by size:
5664 632 459 209 133
TatI w'GTAC_w
Cuts at: 683 1125 3898 6612 683
Size: 442 2773 2714 1168
Fragments arranged by size:
2773 2714 1168 442
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 446 449 478 481 3865 3897 6614 6646 446
Size: 3 29 3 3384 32 2717 32 897
Fragments arranged by size:
3384 2717 897 32 32 29 3 3
Tth111I GACn'n_nGTC
Cuts at: 3370 4894 3370
Size: 1524 5573
XbaI T'CTAG_A
Cuts at: 4128 4128
Size: 7097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3021 4592 3021
Size: 1571 5526
XmaI C'CCGG_G
Cuts at: 2741 4117 2741
Size: 1376 5721
XmnI GAAnn'nnTTC
Cuts at: 350 399 489 1859 2363 2492 350
Size: 49 90 1370 504 129 4955
Fragments arranged by size:
4955 1370 504 129 90 49
ZraI GAC'GTC
Cuts at: 946 946
Size: 7097
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AleI AloI AscI BaeI BbvCI BcgI
BmgBI BmtI Bpu10I BsiWI BsrGI BssHII BstZ17I ClaI
CspCI DraIII FseI FspAI HpaI MfeI NheI NotI
NruI PacI PasI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SrfI SspI StuI SwaI
XhoI