Restriction map for
pF1KSDA0644
(Circular) (Six-base) MAPSORT of: pF1KSDA0644.seq Check: 8386 from: 1 to: 5572
pF1KSDA0644 5572 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 887 1461 887
Size: 574 4998
AccI GT'mk_AC
Cuts at: 2036 2204 2608 2036
Size: 168 404 5000
Fragments arranged by size:
5000 404 168
Acc65I G'GTAC_C
Cuts at: 2586 2586
Size: 5572
AclI AA'CG_TT
Cuts at: 5412 5412
Size: 5572
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1255 3395 3827 4576 4844 1255
Size: 2140 432 749 268 1983
Fragments arranged by size:
2140 1983 749 432 268
AfeI AGC'GCT
Cuts at: 214 1240 1566 214
Size: 1026 326 4220
Fragments arranged by size:
4220 1026 326
AflIII A'CryG_T
Cuts at: 2345 2922 4028 5005 2345
Size: 577 1106 977 2912
Fragments arranged by size:
2912 1106 977 577
AgeI A'CCGG_T
Cuts at: 3960 3960
Size: 5572
AleI CACnn'nnGTG
Cuts at: 2386 2434 2386
Size: 48 5524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2567 2567
Size: 5572
AlwNI CAG_nnn'CTG
Cuts at: 879 1350 2217 2875 4444 879
Size: 471 867 658 1569 2007
Fragments arranged by size:
2007 1569 867 658 471
ApaI G_GGCC'C
Cuts at: 1591 1636 2328 1591
Size: 45 692 4835
Fragments arranged by size:
4835 692 45
ApaLI G'TGCA_C
Cuts at: 246 4342 246
Size: 4096 1476
ApoI r'AATT_y
Cuts at: 2467 2574 4925 5181 2467
Size: 107 2351 256 2858
Fragments arranged by size:
2858 2351 256 107
AseI AT'TA_AT
Cuts at: 20 20
Size: 5572
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5572
AvaI C'yCGr_G
Cuts at: 495 856 1590 1688 2147 2403 2590 495
Size: 361 734 98 459 256 187 3477
Fragments arranged by size:
3477 734 459 361 256 187 98
BamHI G'GATC_C
Cuts at: 695 1360 1371 2595 695
Size: 665 11 1224 3672
Fragments arranged by size:
3672 1224 665 11
BanI G'GyrC_C
Cuts at: 2380 2586 3000 3248 3283 2380
Size: 206 414 248 35 4669
Fragments arranged by size:
4669 414 248 206 35
BanII G_rGCy'C
Cuts at: 200 263 298 1115 1591 1636 1715 2328 2584
Size: 63 35 817 476 45 79 613 256
Cuts at: 2584 3614 200
Size: 1030 2158
Fragments arranged by size:
2158 1030 817 613 476 256 79 63
45 35
BbeI G_GCGC'C
Cuts at: 3004 3252 3004
Size: 248 5324
BbsI GAAGACnn'nnnn_
Cuts at: 639 1275 639
Size: 636 4936
BbvCI CC'TCA_GC
Cuts at: 1383 1383
Size: 5572
BciVI GTATCCnnnnn_n'
Cuts at: 3468 4231 5572 3468
Size: 763 1341 3468
Fragments arranged by size:
3468 1341 763
BclI T'GATC_A
Cuts at: 3090 3090
Size: 5572
BglI GCCn_nnn'nGGC
Cuts at: 326 628 1097 2198 2269 326
Size: 302 469 1101 71 3629
Fragments arranged by size:
3629 1101 469 302 71
BglII A'GATC_T
Cuts at: 3085 5124 3085
Size: 2039 3533
BlpI GC'TnA_GC
Cuts at: 991 1114 1135 2682 991
Size: 123 21 1547 3881
Fragments arranged by size:
3881 1547 123 21
Bme1580I G_kGCm'C
Cuts at: 208 250 275 502 1591 1636 1795 2328 2383
Size: 42 25 227 1089 45 159 533 55
Cuts at: 2383 3195 3288 4346 208
Size: 812 93 1058 1434
Fragments arranged by size:
1434 1089 1058 812 533 227 159 93
55 45 42 25
BmgBI CAC'GTC
Cuts at: 306 886 306
Size: 580 4992
BmrI ACTGGGnnnn_n'
Cuts at: 1812 2782 2863 3198 5344 1812
Size: 970 81 335 2146 2040
Fragments arranged by size:
2146 2040 970 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 459 3937 459
Size: 3478 2094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 703 997 1036 1054 1477 1582 607
Size: 96 294 39 18 423 105 4597
Fragments arranged by size:
4597 423 294 105 96 39 18
Bpu10I CC'TnA_GC
Cuts at: 562 904 1048 1383 562
Size: 342 144 335 4751
Fragments arranged by size:
4751 342 335 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2746 3625 4119 4417 2746
Size: 879 494 298 3901
Fragments arranged by size:
3901 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1465 5276 1465
Size: 3811 1761
BsaAI yAC'GTr
Cuts at: 2562 2923 2562
Size: 361 5211
BsaBI GATnn'nnATC
Cuts at: 6 3084 5123 6
Size: 3078 2039 455
Fragments arranged by size:
3078 2039 455
BsaHI Gr'CG_yC
Cuts at: 1447 2201 2278 3001 3249 3953 5449 1447
Size: 754 77 723 248 704 1496 1570
Fragments arranged by size:
1570 1496 754 723 704 248 77
BsaWI w'CCGG_w
Cuts at: 692 2178 2759 3280 3960 4234 4381 4792 692
Size: 1486 581 521 680 274 147 411 1472
Fragments arranged by size:
1486 1472 680 581 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1713 1743 2335 2365 1713
Size: 30 592 30 4920
Fragments arranged by size:
4920 592 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 810 1264 1453 1483 810
Size: 454 189 30 4899
Fragments arranged by size:
4899 454 189 30
BseYI C'CCAG_C
Cuts at: 233 725 1170 1516 1606 1670 1934 1968 2995
Size: 492 445 346 90 64 264 34 1027
Cuts at: 2995 3753 4332 4828 233
Size: 758 579 496 977
Fragments arranged by size:
1027 977 758 579 496 492 445 346
264 90 64 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 1009 1057 1309 1642 403
Size: 606 48 252 333 4333
Fragments arranged by size:
4333 606 333 252 48
BsiEI CG_ry'CG
Cuts at: 104 1430 1966 2481 3112 3158 4368 104
Size: 1326 536 515 631 46 1210 1308
Fragments arranged by size:
1326 1308 1210 631 536 515 46
BsiHKAI G_wGCw'C
Cuts at: 250 311 1115 1802 1952 2258 2584 3362 3552
Size: 61 804 687 150 306 326 778 190
Cuts at: 3552 4346 250
Size: 794 1476
Fragments arranged by size:
1476 804 794 778 687 326 306 190
150 61
BsmBI CGTCTCn'nnnn_
Cuts at: 892 2285 2517 2542 892
Size: 1393 232 25 3922
Fragments arranged by size:
3922 1393 232 25
Bsp1286I G_dGCh'C
Cuts at: 200 208 250 263 275 298 311 502 1115
Size: 8 42 13 12 23 13 191 613
Cuts at: 1115 1591 1636 1715 1795 1802 1952 2258 2328
Size: 476 45 79 80 7 150 306 70
Cuts at: 2328 2383 2584 3195 3288 3362 3552 3614 4346
Size: 55 201 611 93 74 190 62 732
Cuts at: 4346 200
Size: 1426
Fragments arranged by size:
1426 732 613 611 476 306 201 191
190 150 93 80 79 74 70 62
55 45 42 23 13 13 12 8
7
BspEI T'CCGG_A
Cuts at: 4792 4792
Size: 5572
BspHI T'CATG_A
Cuts at: 4748 4748
Size: 5572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 401 674 887 1219 1447 1461 1664 2048 2620
Size: 273 213 332 228 14 203 384 572
Cuts at: 2620 2834 3136 3517 401
Size: 214 302 381 2456
Fragments arranged by size:
2456 572 384 381 332 302 273 228
214 213 203 14
BsrBI CCG'CTC
Cuts at: 50 413 1052 3862 3916 5402 5567 50
Size: 363 639 2810 54 1486 165 55
Fragments arranged by size:
2810 1486 639 363 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1062 3482 1062
Size: 2420 3152
BsrFI r'CCGG_y
Cuts at: 1100 1220 1268 1753 2383 2420 3568 3749 3960
Size: 120 48 485 630 37 1148 181 211
Cuts at: 3960 1100
Size: 2712
Fragments arranged by size:
2712 1148 630 485 211 181 120 48
37
BssHII G'CGCG_C
Cuts at: 1770 1976 1978 2441 1770
Size: 206 2 463 4901
Fragments arranged by size:
4901 463 206 2
BssSI C'ACGA_G
Cuts at: 157 3841 4201 157
Size: 3684 360 1528
Fragments arranged by size:
3684 1528 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2947 2947
Size: 5572
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5572
BstEII G'GTnAC_C
Cuts at: 2169 2169
Size: 5572
BstXI CCAn_nnnn'nTGG
Cuts at: 1548 1548
Size: 5572
BstYI r'GATC_y
Cuts at: 1 407 695 1188 1360 1371 1695 2595 3062
Size: 406 288 493 172 11 324 900 467
Cuts at: 3062 3085 3420 3666 4680 4766 4778 5124 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
1014 900 493 467 449 406 346 335
324 288 246 172 86 23 12 11
Bsu36I CC'TnA_GG
Cuts at: 935 935
Size: 5572
BtgI C'CryG_G
Cuts at: 125 567 1424 1628 2375 125
Size: 442 857 204 747 3322
Fragments arranged by size:
3322 857 747 442 204
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1730 3520 3701 3864 1730
Size: 1790 181 163 3438
Fragments arranged by size:
3438 1790 181 163
BtsI GCAGTG_nn'
Cuts at: 1297 2154 2813 1297
Size: 857 659 4056
Fragments arranged by size:
4056 857 659
DraI TTT'AAA
Cuts at: 2569 2569
Size: 5572
DraIII CAC_nnn'GTG
Cuts at: 306 2438 306
Size: 2132 3440
DrdI GACnn_nn'nnGTC
Cuts at: 3276 4136 3276
Size: 860 4712
EaeI y'GGCC_r
Cuts at: 191 791 836 1079 1089 1427 1963 2007 2060
Size: 600 45 243 10 338 536 44 53
Cuts at: 2060 2422 3155 3329 3720 3747 5094 191
Size: 362 733 174 391 27 1347 669
Fragments arranged by size:
1347 733 669 600 536 391 362 338
243 174 53 45 44 27 10
EagI C'GGCC_G
Cuts at: 1427 1963 3155 1427
Size: 536 1192 3844
Fragments arranged by size:
3844 1192 536
EarI CTCTTCn'nnn_
Cuts at: 449 3593 3803 449
Size: 3144 210 2218
Fragments arranged by size:
3144 2218 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1056 1975 2328 2382 2470 3778 4090 4236 5382
Size: 919 353 54 88 1308 312 146 1146
Cuts at: 5382 1056
Size: 1246
Fragments arranged by size:
1308 1246 1146 919 353 312 146 88
54
EcoICRI GAG'CTC
Cuts at: 1113 2582 1113
Size: 1469 4103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 703 997 1036 1054 1255 1477 1582 3395
Size: 96 294 39 18 201 222 105 1813
Cuts at: 3395 3827 4576 4844 607
Size: 432 749 268 1335
Fragments arranged by size:
1813 1335 749 432 294 268 222 201
105 96 39 18
EcoNI CCTnn'n_nnAGG
Cuts at: 481 2179 481
Size: 1698 3874
EcoO109I rG'GnC_Cy
Cuts at: 582 957 1587 2324 2325 2709 582
Size: 375 630 737 1 384 3445
Fragments arranged by size:
3445 737 630 384 375 1
EcoRI G'AATT_C
Cuts at: 2467 2574 2467
Size: 107 5465
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 735 759 3589 735
Size: 24 2830 2718
Fragments arranged by size:
2830 2718 24
FspI TGC'GCA
Cuts at: 517 526 1123 3351 517
Size: 9 597 2228 2738
Fragments arranged by size:
2738 2228 597 9
HaeII r_GCGC'y
Cuts at: 216 490 634 1042 1142 1242 1568 1925 3004
Size: 274 144 408 100 100 326 357 1079
Cuts at: 3004 3252 4276 5255 216
Size: 248 1024 979 533
Fragments arranged by size:
1079 1024 979 533 408 357 326 274
248 144 100 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 399 431 1243 1275 1767 1799 1909 1941 3412
Size: 32 812 32 492 32 110 32 1471
Cuts at: 3412 3442 3444 3474 399
Size: 30 2 30 2497
Fragments arranged by size:
2497 1471 812 492 110 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2205 2241 2609 2205
Size: 36 368 5168
Fragments arranged by size:
5168 368 36
KasI G'GCGC_C
Cuts at: 3000 3248 3000
Size: 248 5324
KpnI G_GTAC'C
Cuts at: 2590 2590
Size: 5572
MluI A'CGCG_T
Cuts at: 5005 5005
Size: 5572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 713 1708 1847 1880 4243 4427 713
Size: 995 139 33 2363 184 1858
Fragments arranged by size:
2363 1858 995 184 139 33
MscI TGG'CCA
Cuts at: 2009 3331 2009
Size: 1322 4250
MslI CAynn'nnrTG
Cuts at: 2386 2434 3559 3686 2386
Size: 48 1125 127 4272
Fragments arranged by size:
4272 1125 127 48
MspA1I CmG'CkG
Cuts at: 189 513 569 807 999 1350 1399 1630 1674
Size: 324 56 238 192 351 49 231 44
Cuts at: 1674 1848 1968 2214 2681 2995 3355 4370 4615
Size: 174 120 246 467 314 360 1015 245
Cuts at: 4615 189
Size: 1146
Fragments arranged by size:
1146 1015 467 360 351 324 314 246
245 238 231 192 174 120 56 49
44
NaeI GCC'GGC
Cuts at: 1102 1222 1270 1755 3751 1102
Size: 120 48 485 1996 2923
Fragments arranged by size:
2923 1996 485 120 48
NarI GG'CG_CC
Cuts at: 3001 3249 3001
Size: 248 5324
NcoI C'CATG_G
Cuts at: 125 2375 125
Size: 2250 3322
NgoMIV G'CCGG_C
Cuts at: 1100 1220 1268 1753 3749 1100
Size: 120 48 485 1996 2923
Fragments arranged by size:
2923 1996 485 120 48
NruI TCG'CGA
Cuts at: 3933 3933
Size: 5572
NspI r_CATG'y
Cuts at: 2349 2623 3123 4032 2349
Size: 274 500 909 3889
Fragments arranged by size:
3889 909 500 274
PasI CC'CwG_GG
Cuts at: 624 624
Size: 5572
PciI A'CATG_T
Cuts at: 2345 4028 2345
Size: 1683 3889
PflMI CCAn_nnn'nTGG
Cuts at: 369 369
Size: 5572
PmeI GTTT'AAAC
Cuts at: 2569 2569
Size: 5572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4711 4743 4711
Size: 32 5540
PspOMI G'GGCC_C
Cuts at: 1587 1632 2324 1587
Size: 45 692 4835
Fragments arranged by size:
4835 692 45
PstI C_TGCA'G
Cuts at: 398 851 1458 2546 2617 3302 398
Size: 453 607 1088 71 685 2668
Fragments arranged by size:
2668 1088 685 607 453 71
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5572
PvuII CAG'CTG
Cuts at: 999 1350 2214 2995 3355 999
Size: 351 864 781 360 3216
Fragments arranged by size:
3216 864 781 360 351
RsrII CG'GwC_CG
Cuts at: 3765 3765
Size: 5572
SacI G_AGCT'C
Cuts at: 1115 2584 1115
Size: 1469 4103
SacII CC_GC'GG
Cuts at: 570 1631 570
Size: 1061 4511
SalI G'TCGA_C
Cuts at: 2203 2607 2203
Size: 404 5168
SapI GCTCTTCn'nnn_
Cuts at: 449 3593 3803 449
Size: 3144 210 2218
Fragments arranged by size:
3144 2218 210
SbfI CC_TGCA'GG
Cuts at: 1458 2617 1458
Size: 1159 4413
ScaI AGT'ACT
Cuts at: 5089 5089
Size: 5572
SexAI A'CCwGG_T
Cuts at: 2096 2096
Size: 5572
SfcI C'TryA_G
Cuts at: 33 315 394 769 847 1454 2542 2613 3298
Size: 282 79 375 78 607 1088 71 685
Cuts at: 3298 4293 4484 33
Size: 995 191 1121
Fragments arranged by size:
1121 1088 995 685 607 375 282 191
79 78 71
SfiI GGCCn_nnn'nGGCC
Cuts at: 1097 2269 1097
Size: 1172 4400
SfoI GGC'GCC
Cuts at: 3002 3250 3002
Size: 248 5324
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5572
SgrAI Cr'CCGG_yG
Cuts at: 1753 2383 1753
Size: 630 4942
SmaI CCC'GGG
Cuts at: 497 1592 2149 2592 497
Size: 1095 557 443 3477
Fragments arranged by size:
3477 1095 557 443
SmlI C'TyrA_G
Cuts at: 2725 3640 4134 4396 2725
Size: 915 494 262 3901
Fragments arranged by size:
3901 915 494 262
SnaBI TAC'GTA
Cuts at: 2562 2562
Size: 5572
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5572
SphI G_CATG'C
Cuts at: 2623 3123 2623
Size: 500 5072
SrfI GCCC'GGGC
Cuts at: 1592 1592
Size: 5572
StuI AGG'CCT
Cuts at: 940 1232 940
Size: 292 5280
StyI C'CwwG_G
Cuts at: 125 656 2222 2264 2375 2704 3058 125
Size: 531 1566 42 111 329 354 2639
Fragments arranged by size:
2639 1566 531 354 329 111 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3153 3286 3745 3956 3153
Size: 133 459 211 4769
Fragments arranged by size:
4769 459 211 133
TatI w'GTAC_w
Cuts at: 398 2348 5087 398
Size: 1950 2739 883
Fragments arranged by size:
2739 1950 883
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2335 2367 5089 5121 2335
Size: 32 2722 32 2786
Fragments arranged by size:
2786 2722 32 32
Tth111I GACn'n_nGTC
Cuts at: 643 3367 643
Size: 2724 2848
XbaI T'CTAG_A
Cuts at: 1191 2601 1191
Size: 1410 4162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1825 3065 1825
Size: 1240 4332
XmaI C'CCGG_G
Cuts at: 495 1590 2147 2590 495
Size: 1095 557 443 3477
Fragments arranged by size:
3477 1095 557 443
XmnI GAAnn'nnTTC
Cuts at: 718 718
Size: 5572
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI
PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BcgI
BfrBI BmtI BsiWI BsmI BsrGI BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NsiI PacI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SanDI SspI SwaI XhoI ZraI