Restriction map for
pF1KSDA0645
(Circular) (Six-base) MAPSORT of: pF1KSDA0645.seq Check: 9955 from: 1 to: 7855
pF1KSDA0645 7855 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1573 1751 1573
Size: 178 7677
AccI GT'mk_AC
Cuts at: 143 395 4891 143
Size: 252 4496 3107
Fragments arranged by size:
4496 3107 252
Acc65I G'GTAC_C
Cuts at: 4869 4869
Size: 7855
AclI AA'CG_TT
Cuts at: 2674 7695 2674
Size: 5021 2834
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2163 2713 2880 2909 2935 4771 5678 6110 6859
Size: 550 167 29 26 1836 907 432 749
Cuts at: 6859 7127 2163
Size: 268 2891
Fragments arranged by size:
2891 1836 907 749 550 432 268 167
29 26
AflII C'TTAA_G
Cuts at: 298 298
Size: 7855
AflIII A'CryG_T
Cuts at: 496 4643 5205 6311 7288 496
Size: 4147 562 1106 977 1063
Fragments arranged by size:
4147 1106 1063 977 562
AgeI A'CCGG_T
Cuts at: 6243 6243
Size: 7855
AhdI GACnn_n'nnGTC
Cuts at: 2163 2331 2163
Size: 168 7687
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1591 4850 1591
Size: 3259 4596
AlwNI CAG_nnn'CTG
Cuts at: 1616 2394 2774 3528 5158 6727 1616
Size: 778 380 754 1630 1569 2744
Fragments arranged by size:
2744 1630 1569 778 754 380
ApaI G_GGCC'C
Cuts at: 1551 3158 1551
Size: 1607 6248
ApaLI G'TGCA_C
Cuts at: 1835 3943 6625 1835
Size: 2108 2682 3065
Fragments arranged by size:
3065 2682 2108
ApoI r'AATT_y
Cuts at: 823 2818 4857 7208 7464 823
Size: 1995 2039 2351 256 1214
Fragments arranged by size:
2351 2039 1995 1214 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7855
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7855
AvaI C'yCGr_G
Cuts at: 1138 1453 1544 1787 4873 1138
Size: 315 91 243 3086 4120
Fragments arranged by size:
4120 3086 315 243 91
AvrII C'CTAG_G
Cuts at: 1701 2239 1701
Size: 538 7317
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 593 626 3655 3688 593
Size: 33 3029 33 4760
Fragments arranged by size:
4760 3029 33 33
BamHI G'GATC_C
Cuts at: 2073 3692 4878 2073
Size: 1619 1186 5050
Fragments arranged by size:
5050 1619 1186
BanI G'GyrC_C
Cuts at: 3400 4869 5283 5531 5566 3400
Size: 1469 414 248 35 5689
Fragments arranged by size:
5689 1469 414 248 35
BanII G_rGCy'C
Cuts at: 1551 3158 3298 3830 3941 4867 5897 1551
Size: 1607 140 532 111 926 1030 3509
Fragments arranged by size:
3509 1607 1030 926 532 140 111
BbeI G_GCGC'C
Cuts at: 5287 5535 5287
Size: 248 7607
BbsI GAAGACnn'nnnn_
Cuts at: 887 2897 887
Size: 2010 5845
BbvCI CC'TCA_GC
Cuts at: 1026 3213 1026
Size: 2187 5668
BciVI GTATCCnnnnn_n'
Cuts at: 977 1768 1888 2744 5751 6514 7855 977
Size: 791 120 856 3007 763 1341 977
Fragments arranged by size:
3007 1341 977 856 791 763 120
BclI T'GATC_A
Cuts at: 1130 2705 5373 1130
Size: 1575 2668 3612
Fragments arranged by size:
3612 2668 1575
BfrBI ATG'CAT
Cuts at: 3760 4827 3760
Size: 1067 6788
BglI GCCn_nnn'nGGC
Cuts at: 3928 3987 3928
Size: 59 7796
BglII A'GATC_T
Cuts at: 1570 5368 7407 1570
Size: 3798 2039 2018
Fragments arranged by size:
3798 2039 2018
BlpI GC'TnA_GC
Cuts at: 2212 2587 4965 2212
Size: 375 2378 5102
Fragments arranged by size:
5102 2378 375
Bme1580I G_kGCm'C
Cuts at: 1551 1839 3158 3947 4344 4839 5478 5571 6629
Size: 288 1319 789 397 495 639 93 1058
Cuts at: 6629 1551
Size: 2777
Fragments arranged by size:
2777 1319 1058 789 639 495 397 288
93
BmgBI CAC'GTC
Cuts at: 4110 4110
Size: 7855
BmrI ACTGGGnnnn_n'
Cuts at: 1121 1545 3161 3187 3297 4279 4307 4437 4683
Size: 424 1616 26 110 982 28 130 246
Cuts at: 4683 5065 5146 5481 7627 1121
Size: 382 81 335 2146 1349
Fragments arranged by size:
2146 1616 1349 982 424 382 335 246
130 110 81 28 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2820 6220 2820
Size: 3400 4455
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1066 1815 1819 2122 2270 2857 2943 3196 3489
Size: 749 4 303 148 587 86 253 293
Cuts at: 3489 4153 4695 4837 1066
Size: 664 542 142 4084
Fragments arranged by size:
4084 749 664 587 542 303 293 253
148 142 86 4
Bpu10I CC'TnA_GC
Cuts at: 1026 3213 1026
Size: 2187 5668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 992 1502 4363 5029 5908 6402 6700 332
Size: 660 510 2861 666 879 494 298 1487
Fragments arranged by size:
2861 1487 879 666 660 510 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 357 7559 357
Size: 7202 653
BsaAI yAC'GTr
Cuts at: 3684 4845 5206 3684
Size: 1161 361 6333
Fragments arranged by size:
6333 1161 361
BsaBI GATnn'nnATC
Cuts at: 6 5367 7406 6
Size: 5361 2039 455
Fragments arranged by size:
5361 2039 455
BsaHI Gr'CG_yC
Cuts at: 1505 5284 5532 6236 7732 1505
Size: 3779 248 704 1496 1628
Fragments arranged by size:
3779 1628 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2002 5042 5563 6243 6517 6664 7075 2002
Size: 3040 521 680 274 147 411 2782
Fragments arranged by size:
3040 2782 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 224 254 1681 1711 2635 2665 2833 2863 3369
Size: 30 1427 30 924 30 168 30 506
Cuts at: 3369 3399 224
Size: 30 4680
Fragments arranged by size:
4680 1427 924 506 168 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1689 2076 2206 2653 2656 2854 3483 4669 1689
Size: 387 130 447 3 198 629 1186 4875
Fragments arranged by size:
4875 1186 629 447 387 198 130 3
BseYI C'CCAG_C
Cuts at: 2483 2810 4343 5278 6036 6615 7111 2483
Size: 327 1533 935 758 579 496 3227
Fragments arranged by size:
3227 1533 935 758 579 496 327
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1729 1883 2246 2491 2563 3041 3170 4161
Size: 807 154 363 245 72 478 129 991
Cuts at: 4161 4180 922
Size: 19 4597
Fragments arranged by size:
4597 991 807 478 363 245 154 129
72 19
BsiEI CG_ry'CG
Cuts at: 104 3834 5395 5441 6651 104
Size: 3730 1561 46 1210 1308
Fragments arranged by size:
3730 1561 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 543 1287 1839 1914 3298 3941 3947 4397 4867
Size: 744 552 75 1384 643 6 450 470
Cuts at: 4867 5645 5835 6629 543
Size: 778 190 794 1769
Fragments arranged by size:
1769 1384 794 778 744 643 552 470
450 190 75 6
BsiWI C'GTAC_G
Cuts at: 4841 4841
Size: 7855
BsmI GAATG_Cn'
Cuts at: 3083 3168 4179 4442 3083
Size: 85 1011 263 6496
Fragments arranged by size:
6496 1011 263 85
BsmBI CGTCTCn'nnnn_
Cuts at: 2521 2808 2521
Size: 287 7568
Bsp1286I G_dGCh'C
Cuts at: 543 1287 1551 1839 1914 3158 3298 3830 3941
Size: 744 264 288 75 1244 140 532 111
Cuts at: 3941 3947 4344 4397 4839 4867 5478 5571 5645
Size: 6 397 53 442 28 611 93 74
Cuts at: 5645 5835 5897 6629 543
Size: 190 62 732 1769
Fragments arranged by size:
1769 1244 744 732 611 532 442 397
288 264 190 140 111 93 75 74
62 53 28 6
BspEI T'CCGG_A
Cuts at: 2002 7075 2002
Size: 5073 2782
BspHI T'CATG_A
Cuts at: 765 1172 2115 7031 765
Size: 407 943 4916 1589
Fragments arranged by size:
4916 1589 943 407
BspMI ACCTGCnnnn'nnnn_
Cuts at: 276 1573 1751 2218 2478 4903 5117 5419 5800
Size: 1297 178 467 260 2425 214 302 381
Cuts at: 5800 276
Size: 2331
Fragments arranged by size:
2425 2331 1297 467 381 302 260 214
178
BsrBI CCG'CTC
Cuts at: 50 4659 6145 6199 7685 7850 50
Size: 4609 1486 54 1486 165 55
Fragments arranged by size:
4609 1486 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4457 5765 4457
Size: 1308 6547
BsrFI r'CCGG_y
Cuts at: 2881 3986 4127 4787 5851 6032 6243 2881
Size: 1105 141 660 1064 181 211 4493
Fragments arranged by size:
4493 1105 1064 660 211 181 141
BsrGI T'GTAC_A
Cuts at: 4479 4479
Size: 7855
BssSI C'ACGA_G
Cuts at: 3940 6124 6484 3940
Size: 2184 360 5311
Fragments arranged by size:
5311 2184 360
BstAPI GCAn_nnn'nTGC
Cuts at: 3732 5230 3732
Size: 1498 6357
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7855
BstEII G'GTnAC_C
Cuts at: 4209 4209
Size: 7855
BstXI CCAn_nnnn'nTGG
Cuts at: 3345 3457 3345
Size: 112 7743
BstYI r'GATC_y
Cuts at: 1 1570 2018 2073 2753 3569 3692 4878 5345
Size: 1569 448 55 680 816 123 1186 467
Cuts at: 5345 5368 5703 5949 6963 7049 7061 7407 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
1569 1186 1014 816 680 467 449 448
346 335 246 123 86 55 23 12
Bsu36I CC'TnA_GG
Cuts at: 401 2636 401
Size: 2235 5620
BtgI C'CryG_G
Cuts at: 456 3431 4155 456
Size: 2975 724 4156
Fragments arranged by size:
4156 2975 724
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1322 3082 3721 5803 5984 6147 1322
Size: 1760 639 2082 181 163 3030
Fragments arranged by size:
3030 2082 1760 639 181 163
BtsI GCAGTG_nn'
Cuts at: 186 1654 1866 2991 4306 5096 186
Size: 1468 212 1125 1315 790 2945
Fragments arranged by size:
2945 1468 1315 1125 790 212
DraI TTT'AAA
Cuts at: 4852 4852
Size: 7855
DraIII CAC_nnn'GTG
Cuts at: 2685 2685
Size: 7855
DrdI GACnn_nn'nnGTC
Cuts at: 5559 6419 5559
Size: 860 6995
EaeI y'GGCC_r
Cuts at: 453 1437 3710 3839 3988 5438 5612 6003 6030
Size: 984 2273 129 149 1450 174 391 27
Cuts at: 6030 7377 453
Size: 1347 931
Fragments arranged by size:
2273 1450 1347 984 931 391 174 149
129 27
EagI C'GGCC_G
Cuts at: 5438 5438
Size: 7855
EarI CTCTTCn'nnn_
Cuts at: 909 961 2492 3286 3734 3929 4231 4354 4450
Size: 52 1531 794 448 195 302 123 96
Cuts at: 4450 5876 6086 909
Size: 1426 210 2678
Fragments arranged by size:
2678 1531 1426 794 448 302 210 195
123 96 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2062 3288 3792 4629 6061 6373 6519 7665 2062
Size: 1226 504 837 1432 312 146 1146 2252
Fragments arranged by size:
2252 1432 1226 1146 837 504 312 146
EcoICRI GAG'CTC
Cuts at: 3296 3939 4865 3296
Size: 643 926 6286
Fragments arranged by size:
6286 926 643
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1066 1815 1819 2122 2163 2270 2713 2857 2880
Size: 749 4 303 41 107 443 144 23
Cuts at: 2880 2909 2935 2943 3196 3489 4153 4695 4771
Size: 29 26 8 253 293 664 542 76
Cuts at: 4771 4837 5678 6110 6859 7127 1066
Size: 66 841 432 749 268 1794
Fragments arranged by size:
1794 841 749 749 664 542 443 432
303 293 268 253 144 107 76 66
41 29 26 23 8 4
EcoNI CCTnn'n_nnAGG
Cuts at: 4447 4447
Size: 7855
EcoO109I rG'GnC_Cy
Cuts at: 2243 2391 2927 3014 3627 4297 4992 2243
Size: 148 536 87 613 670 695 5106
Fragments arranged by size:
5106 695 670 613 536 148 87
EcoRI G'AATT_C
Cuts at: 2818 4857 2818
Size: 2039 5816
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 186 5872 186
Size: 5686 2169
FspI TGC'GCA
Cuts at: 5634 5634
Size: 7855
HaeII r_GCGC'y
Cuts at: 1974 4710 4718 5287 5535 6559 7538 1974
Size: 2736 8 569 248 1024 979 2291
Fragments arranged by size:
2736 2291 1024 979 569 248 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 330 354 362 386 796 828 1581 1612 1613
Size: 24 8 24 410 32 753 31 1
Cuts at: 1613 1644 1681 1713 1997 2029 2149 2181 2317
Size: 31 37 32 284 32 120 32 136
Cuts at: 2317 2349 3502 3534 5695 5725 5727 5757 330
Size: 32 1153 32 2161 30 2 30 2428
Fragments arranged by size:
2428 2161 1153 753 410 284 136 120
37 32 32 32 32 32 32 31
31 30 30 24 24 8 2 1
HincII GTy'rAC
Cuts at: 3182 4107 4892 3182
Size: 925 785 6145
Fragments arranged by size:
6145 925 785
HindIII A'AGCT_T
Cuts at: 226 1951 226
Size: 1725 6130
KasI G'GCGC_C
Cuts at: 5283 5531 5283
Size: 248 7607
KpnI G_GTAC'C
Cuts at: 4873 4873
Size: 7855
MluI A'CGCG_T
Cuts at: 7288 7288
Size: 7855
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1985 2101 2298 3212 3374 6526 6710 1985
Size: 116 197 914 162 3152 184 3130
Fragments arranged by size:
3152 3130 914 197 184 162 116
MscI TGG'CCA
Cuts at: 3712 3841 5614 3712
Size: 129 1773 5953
Fragments arranged by size:
5953 1773 129
MslI CAynn'nnrTG
Cuts at: 1723 1751 3343 3388 4832 5842 5969 1723
Size: 28 1592 45 1444 1010 127 3609
Fragments arranged by size:
3609 1592 1444 1010 127 45 28
MspA1I CmG'CkG
Cuts at: 1191 2063 2586 3277 3528 3609 4145 4370 4569
Size: 872 523 691 251 81 536 225 199
Cuts at: 4569 4605 4964 5278 5638 6653 6898 1191
Size: 36 359 314 360 1015 245 2148
Fragments arranged by size:
2148 1015 872 691 536 523 360 359
314 251 245 225 199 81 36
NaeI GCC'GGC
Cuts at: 2883 3988 6034 2883
Size: 1105 2046 4704
Fragments arranged by size:
4704 2046 1105
NarI GG'CG_CC
Cuts at: 5284 5532 5284
Size: 248 7607
NcoI C'CATG_G
Cuts at: 3431 4155 3431
Size: 724 7131
NdeI CA'TA_TG
Cuts at: 3415 3415
Size: 7855
NgoMIV G'CCGG_C
Cuts at: 2881 3986 6032 2881
Size: 1105 2046 4704
Fragments arranged by size:
4704 2046 1105
NruI TCG'CGA
Cuts at: 6216 6216
Size: 7855
NsiI A_TGCA'T
Cuts at: 3762 4829 3762
Size: 1067 6788
NspI r_CATG'y
Cuts at: 500 1252 1882 2166 3760 4647 4831 4906 5406
Size: 752 630 284 1594 887 184 75 500
Cuts at: 5406 6315 500
Size: 909 2040
Fragments arranged by size:
2040 1594 909 887 752 630 500 284
184 75
PciI A'CATG_T
Cuts at: 496 4643 6311 496
Size: 4147 1668 2040
Fragments arranged by size:
4147 2040 1668
PflMI CCAn_nnn'nTGG
Cuts at: 519 3432 3863 4703 519
Size: 2913 431 840 3671
Fragments arranged by size:
3671 2913 840 431
PfoI T'CCnGG_A
Cuts at: 1796 3044 3572 4448 1796
Size: 1248 528 876 5203
Fragments arranged by size:
5203 1248 876 528
PmeI GTTT'AAAC
Cuts at: 4852 4852
Size: 7855
PmlI CAC'GTG
Cuts at: 3684 3684
Size: 7855
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3201 3233 6994 7026 3201
Size: 32 3761 32 4030
Fragments arranged by size:
4030 3761 32 32
PpuMI rG'GwC_Cy
Cuts at: 2243 2927 2243
Size: 684 7171
PshAI GACnn'nnGTC
Cuts at: 778 3601 778
Size: 2823 5032
PspOMI G'GGCC_C
Cuts at: 1547 3154 1547
Size: 1607 6248
PstI C_TGCA'G
Cuts at: 1570 2094 2399 4568 4900 5585 1570
Size: 524 305 2169 332 685 3840
Fragments arranged by size:
3840 2169 685 524 332 305
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7855
PvuII CAG'CTG
Cuts at: 3277 3528 3609 4145 4370 4569 5278 5638 3277
Size: 251 81 536 225 199 709 360 5494
Fragments arranged by size:
5494 709 536 360 251 225 199 81
RsrII CG'GwC_CG
Cuts at: 6048 6048
Size: 7855
SacI G_AGCT'C
Cuts at: 3298 3941 4867 3298
Size: 643 926 6286
Fragments arranged by size:
6286 926 643
SalI G'TCGA_C
Cuts at: 4890 4890
Size: 7855
SapI GCTCTTCn'nnn_
Cuts at: 3286 3734 3929 4354 5876 6086 3286
Size: 448 195 425 1522 210 5055
Fragments arranged by size:
5055 1522 448 425 210 195
SbfI CC_TGCA'GG
Cuts at: 4900 4900
Size: 7855
ScaI AGT'ACT
Cuts at: 4223 7372 4223
Size: 3149 4706
SexAI A'CCwGG_T
Cuts at: 1887 1887
Size: 7855
SfcI C'TryA_G
Cuts at: 33 759 1566 2090 2395 2604 2766 4564 4896
Size: 726 807 524 305 209 162 1798 332
Cuts at: 4896 5581 6576 6767 33
Size: 685 995 191 1121
Fragments arranged by size:
1798 1121 995 807 726 685 524 332
305 209 191 162
SfoI GGC'GCC
Cuts at: 5285 5533 5285
Size: 248 7607
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7855
SmaI CCC'GGG
Cuts at: 1140 1546 4875 1140
Size: 406 3329 4120
Fragments arranged by size:
4120 3329 406
SmlI C'TyrA_G
Cuts at: 298 347 1007 1517 1787 4378 5008 5923 6417
Size: 49 660 510 270 2591 630 915 494
Cuts at: 6417 6679 298
Size: 262 1474
Fragments arranged by size:
2591 1474 915 660 630 510 494 270
262 49
SnaBI TAC'GTA
Cuts at: 4845 4845
Size: 7855
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7855
SphI G_CATG'C
Cuts at: 4831 4906 5406 4831
Size: 75 500 7280
Fragments arranged by size:
7280 500 75
SspI AAT'ATT
Cuts at: 4492 4492
Size: 7855
StuI AGG'CCT
Cuts at: 2615 2615
Size: 7855
StyI C'CwwG_G
Cuts at: 1701 1769 2239 3431 4155 4239 4987 5341 1701
Size: 68 470 1192 724 84 748 354 4215
Fragments arranged by size:
4215 1192 748 724 470 354 84 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 526 1121 1135 2802 3848 5436 5569 6028 6239
Size: 595 14 1667 1046 1588 133 459 211
Cuts at: 6239 526
Size: 2142
Fragments arranged by size:
2142 1667 1588 1046 595 459 211 133
14
TatI w'GTAC_w
Cuts at: 2843 4221 4479 7370 2843
Size: 1378 258 2891 3328
Fragments arranged by size:
3328 2891 1378 258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1199 1210 1231 1242 2125 2157 2833 2865 7372
Size: 11 21 11 883 32 676 32 4507
Cuts at: 7372 7404 1199
Size: 32 1650
Fragments arranged by size:
4507 1650 883 676 32 32 32 21
11 11
Tth111I GACn'n_nGTC
Cuts at: 5650 5650
Size: 7855
XbaI T'CTAG_A
Cuts at: 790 2262 4884 790
Size: 1472 2622 3761
Fragments arranged by size:
3761 2622 1472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3171 3390 5348 3171
Size: 219 1958 5678
Fragments arranged by size:
5678 1958 219
XhoI C'TCGA_G
Cuts at: 1787 1787
Size: 7855
XmaI C'CCGG_G
Cuts at: 1138 1544 4873 1138
Size: 406 3329 4120
Fragments arranged by size:
4120 3329 406
XmnI GAAnn'nnTTC
Cuts at: 718 4645 4756 718
Size: 3927 111 3817
Fragments arranged by size:
3927 3817 111
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NruI NsiI NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AleI AloI AscI BcgI BmtI BssHII
BstZ17I ClaI CspCI EcoRV FseI FspAI HpaI MfeI
NheI NotI PacI PasI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SrfI SwaI ZraI