Restriction map for
pF1KSDA0647
(Circular) (Six-base) MAPSORT of: pF1KSDA0647.seq Check: 203 from: 1 to: 6724
pF1KSDA0647 6724 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 597 1313 3246 3760 597
Size: 716 1933 514 3561
Fragments arranged by size:
3561 1933 716 514
Acc65I G'GTAC_C
Cuts at: 3738 3738
Size: 6724
AclI AA'CG_TT
Cuts at: 6564 6564
Size: 6724
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 518 3247 3595 3703 4547 4979 5728 5996
Size: 319 2729 348 108 844 432 749 268
Cuts at: 5996 199
Size: 927
Fragments arranged by size:
2729 927 844 749 432 348 319 268
108
AflII C'TTAA_G
Cuts at: 1567 1567
Size: 6724
AflIII A'CryG_T
Cuts at: 3337 4074 5180 6157 3337
Size: 737 1106 977 3904
Fragments arranged by size:
3904 1106 977 737
AgeI A'CCGG_T
Cuts at: 5112 5112
Size: 6724
AleI CACnn'nnGTG
Cuts at: 3181 3290 3181
Size: 109 6615
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3498 3498
Size: 6724
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 733 765 733
Size: 32 6692
AlwNI CAG_nnn'CTG
Cuts at: 702 2404 2644 4027 5596 702
Size: 1702 240 1383 1569 1830
Fragments arranged by size:
1830 1702 1569 1383 240
ApaI G_GGCC'C
Cuts at: 143 1812 2966 143
Size: 1669 1154 3901
Fragments arranged by size:
3901 1669 1154
ApaLI G'TGCA_C
Cuts at: 2411 5494 2411
Size: 3083 3641
ApoI r'AATT_y
Cuts at: 313 1591 3502 3726 6077 6333 313
Size: 1278 1911 224 2351 256 704
Fragments arranged by size:
2351 1911 1278 704 256 224
AseI AT'TA_AT
Cuts at: 20 20
Size: 6724
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6724
AvaI C'yCGr_G
Cuts at: 912 2255 3742 912
Size: 1343 1487 3894
Fragments arranged by size:
3894 1487 1343
BamHI G'GATC_C
Cuts at: 1218 3747 1218
Size: 2529 4195
BanI G'GyrC_C
Cuts at: 419 530 1648 3738 4152 4400 4435 419
Size: 111 1118 2090 414 248 35 2708
Fragments arranged by size:
2708 2090 1118 414 248 111 35
BanII G_rGCy'C
Cuts at: 143 937 1812 2063 2966 2988 3311 3736 4766
Size: 794 875 251 903 22 323 425 1030
Cuts at: 4766 143
Size: 2101
Fragments arranged by size:
2101 1030 903 875 794 425 323 251
22
BbeI G_GCGC'C
Cuts at: 4156 4404 4156
Size: 248 6476
BbsI GAAGACnn'nnnn_
Cuts at: 506 2140 2459 2714 3401 3528 506
Size: 1634 319 255 687 127 3702
Fragments arranged by size:
3702 1634 687 319 255 127
BbvCI CC'TCA_GC
Cuts at: 940 2123 2557 940
Size: 1183 434 5107
Fragments arranged by size:
5107 1183 434
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3226 3260 3226
Size: 34 6690
BciVI GTATCCnnnnn_n'
Cuts at: 4620 5383 6724 4620
Size: 763 1341 4620
Fragments arranged by size:
4620 1341 763
BclI T'GATC_A
Cuts at: 2547 3384 3591 4242 2547
Size: 837 207 651 5029
Fragments arranged by size:
5029 837 651 207
BglI GCCn_nnn'nGGC
Cuts at: 1087 1646 1087
Size: 559 6165
BglII A'GATC_T
Cuts at: 4237 6276 4237
Size: 2039 4685
BlpI GC'TnA_GC
Cuts at: 466 3834 466
Size: 3368 3356
Bme1580I G_kGCm'C
Cuts at: 143 685 906 1469 1812 2415 2531 2966 3297
Size: 542 221 563 343 603 116 435 331
Cuts at: 3297 4347 4440 5498 143
Size: 1050 93 1058 1369
Fragments arranged by size:
1369 1058 1050 603 563 542 435 343
331 221 116 93
BmgBI CAC'GTC
Cuts at: 633 884 633
Size: 251 6473
BmrI ACTGGGnnnn_n'
Cuts at: 680 938 1888 2853 3604 3934 4015 4350 6496
Size: 258 950 965 751 330 81 335 2146
Cuts at: 6496 680
Size: 908
Fragments arranged by size:
2146 965 950 908 751 335 330 258
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 474 5089 474
Size: 4615 2109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 666 757 1031 1264 1522 2077 2306 3116
Size: 494 91 274 233 258 555 229 810
Cuts at: 3116 3284 172
Size: 168 3612
Fragments arranged by size:
3612 810 555 494 274 258 233 229
168 91
Bpu10I CC'TnA_GC
Cuts at: 940 2123 2557 940
Size: 1183 434 5107
Fragments arranged by size:
5107 1183 434
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2205 3898 4777 5271 5569 2205
Size: 1693 879 494 298 3360
Fragments arranged by size:
3360 1693 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 6428 6428
Size: 6724
BsaAI yAC'GTr
Cuts at: 3714 4075 3714
Size: 361 6363
BsaBI GATnn'nnATC
Cuts at: 6 4236 6275 6
Size: 4230 2039 455
Fragments arranged by size:
4230 2039 455
BsaHI Gr'CG_yC
Cuts at: 4153 4401 5105 6601 4153
Size: 248 704 1496 4276
Fragments arranged by size:
4276 1496 704 248
BsaWI w'CCGG_w
Cuts at: 344 1172 2789 3911 4432 5112 5386 5533 5944
Size: 828 1617 1122 521 680 274 147 411
Cuts at: 5944 344
Size: 1124
Fragments arranged by size:
1617 1124 1122 828 680 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1014 1044 1618 1648 1014
Size: 30 574 30 6090
Fragments arranged by size:
6090 574 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 562 1022 2144 2147 2588 3131 148
Size: 414 460 1122 3 441 543 3741
Fragments arranged by size:
3741 1122 543 460 441 414 3
BseYI C'CCAG_C
Cuts at: 145 1355 1768 2164 2346 2504 3416 3455 4147
Size: 1210 413 396 182 158 912 39 692
Cuts at: 4147 4905 5484 5980 145
Size: 758 579 496 889
Fragments arranged by size:
1210 912 889 758 692 579 496 413
396 182 158 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1798 655
Size: 1143 5581
BsiEI CG_ry'CG
Cuts at: 104 4264 4310 5520 104
Size: 4160 46 1210 1308
Fragments arranged by size:
4160 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 587 1009 1024 2415 3736 4514 4704 5498 587
Size: 422 15 1391 1321 778 190 794 1813
Fragments arranged by size:
1813 1391 1321 794 778 422 190 15
BsmI GAATG_Cn'
Cuts at: 1603 1603
Size: 6724
Bsp1286I G_dGCh'C
Cuts at: 143 587 685 906 937 1009 1024 1469 1812
Size: 444 98 221 31 72 15 445 343
Cuts at: 1812 2063 2415 2531 2966 2988 3297 3311 3736
Size: 251 352 116 435 22 309 14 425
Cuts at: 3736 4347 4440 4514 4704 4766 5498 143
Size: 611 93 74 190 62 732 1369
Fragments arranged by size:
1369 732 611 445 444 435 425 352
343 309 251 221 190 116 98 93
74 72 62 31 22 15 14
BspEI T'CCGG_A
Cuts at: 344 1172 2789 5944 344
Size: 828 1617 3155 1124
Fragments arranged by size:
3155 1617 1124 828
BspHI T'CATG_A
Cuts at: 3668 5900 3668
Size: 2232 4492
BspMI ACCTGCnnnn'nnnn_
Cuts at: 495 1714 3772 3986 4288 4669 495
Size: 1219 2058 214 302 381 2550
Fragments arranged by size:
2550 2058 1219 381 302 214
BsrBI CCG'CTC
Cuts at: 50 758 1925 5014 5068 6554 6719 50
Size: 708 1167 3089 54 1486 165 55
Fragments arranged by size:
3089 1486 1167 708 165 55 54
BsrDI GCAATG_nn'
Cuts at: 281 807 864 887 3528 3692 4634 281
Size: 526 57 23 2641 164 942 2371
Fragments arranged by size:
2641 2371 942 526 164 57 23
BsrFI r'CCGG_y
Cuts at: 299 1325 4720 4901 5112 299
Size: 1026 3395 181 211 1911
Fragments arranged by size:
3395 1911 1026 211 181
BssSI C'ACGA_G
Cuts at: 3653 4993 5353 3653
Size: 1340 360 5024
Fragments arranged by size:
5024 1340 360
BstAPI GCAn_nnn'nTGC
Cuts at: 274 487 2567 4099 274
Size: 213 2080 1532 2899
Fragments arranged by size:
2899 2080 1532 213
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6724
BstXI CCAn_nnnn'nTGG
Cuts at: 1956 1956
Size: 6724
BstYI r'GATC_y
Cuts at: 1 171 1218 1878 1951 2631 2688 3747 4214
Size: 170 1047 660 73 680 57 1059 467
Cuts at: 4214 4237 4572 4818 5832 5918 5930 6276 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
1059 1047 1014 680 660 467 449 346
335 246 170 86 73 57 23 12
Bsu36I CC'TnA_GG
Cuts at: 2078 2637 2078
Size: 559 6165
BtgI C'CryG_G
Cuts at: 125 776 1955 2697 3176 3314 125
Size: 651 1179 742 479 138 3535
Fragments arranged by size:
3535 1179 742 651 479 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 798 4672 4853 5016 798
Size: 3874 181 163 2506
Fragments arranged by size:
3874 2506 181 163
BtsI GCAGTG_nn'
Cuts at: 448 1082 1341 2255 3285 3366 3480 3965 448
Size: 634 259 914 1030 81 114 485 3207
Fragments arranged by size:
3207 1030 914 634 485 259 114 81
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2081 2116 2081
Size: 35 6689
DraI TTT'AAA
Cuts at: 3721 3721
Size: 6724
DraIII CAC_nnn'GTG
Cuts at: 2581 2581
Size: 6724
DrdI GACnn_nn'nnGTC
Cuts at: 4428 5288 4428
Size: 860 5864
EaeI y'GGCC_r
Cuts at: 1079 1154 1385 1488 1658 1920 3512 4307 4481
Size: 75 231 103 170 262 1592 795 174
Cuts at: 4481 4872 4899 6246 1079
Size: 391 27 1347 1557
Fragments arranged by size:
1592 1557 1347 795 391 262 231 174
170 103 75 27
EagI C'GGCC_G
Cuts at: 4307 4307
Size: 6724
EarI CTCTTCn'nnn_
Cuts at: 200 1109 2260 2297 4745 4955 200
Size: 909 1151 37 2448 210 1969
Fragments arranged by size:
2448 1969 1151 909 210 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 622 4930 5242 5388 6534 622
Size: 4308 312 146 1146 812
Fragments arranged by size:
4308 1146 812 312 146
EcoICRI GAG'CTC
Cuts at: 3734 3734
Size: 6724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 199 518 666 757 1031 1264 1522 2077
Size: 27 319 148 91 274 233 258 555
Cuts at: 2077 2306 3116 3247 3284 3595 3703 4547 4979
Size: 229 810 131 37 311 108 844 432
Cuts at: 4979 5728 5996 172
Size: 749 268 900
Fragments arranged by size:
900 844 810 749 555 432 319 311
274 268 258 233 229 148 131 108
91 37 27
EcoO109I rG'GnC_Cy
Cuts at: 139 140 219 1809 2119 2962 2963 3861 139
Size: 1 79 1590 310 843 1 898 3002
Fragments arranged by size:
3002 1590 898 843 310 79 1 1
EcoRI G'AATT_C
Cuts at: 3502 3726 3502
Size: 224 6500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 229 2164 3418 4741 229
Size: 1935 1254 1323 2212
Fragments arranged by size:
2212 1935 1323 1254
FseI GG_CCGG'CC
Cuts at: 1329 1329
Size: 6724
FspI TGC'GCA
Cuts at: 799 4503 799
Size: 3704 3020
HaeII r_GCGC'y
Cuts at: 1723 4156 4404 5428 6407 1723
Size: 2433 248 1024 979 2040
Fragments arranged by size:
2433 2040 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1045 1077 1203 1235 3364 3396 4564 4594 4596
Size: 32 126 32 2129 32 1168 30 2
Cuts at: 4596 4626 1045
Size: 30 3143
Fragments arranged by size:
3143 2129 1168 126 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 598 3247 3761 598
Size: 2649 514 3561
Fragments arranged by size:
3561 2649 514
HindIII A'AGCT_T
Cuts at: 2094 2094
Size: 6724
KasI G'GCGC_C
Cuts at: 4152 4400 4152
Size: 248 6476
KpnI G_GTAC'C
Cuts at: 3742 3742
Size: 6724
MluI A'CGCG_T
Cuts at: 6157 6157
Size: 6724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1400 2864 5395 5579 1400
Size: 1464 2531 184 2545
Fragments arranged by size:
2545 2531 1464 184
MscI TGG'CCA
Cuts at: 1081 1156 1387 1490 1660 3514 4483 1081
Size: 75 231 103 170 1854 969 3322
Fragments arranged by size:
3322 1854 969 231 170 103 75
MslI CAynn'nnrTG
Cuts at: 1150 1851 3029 3181 3290 4711 4838 1150
Size: 701 1178 152 109 1421 127 3036
Fragments arranged by size:
3036 1421 1178 701 152 127 109
MspA1I CmG'CkG
Cuts at: 821 842 1291 1828 3681 3833 4147 4507 5522
Size: 21 449 537 1853 152 314 360 1015
Cuts at: 5522 5767 821
Size: 245 1778
Fragments arranged by size:
1853 1778 1015 537 449 360 314 245
152 21
NaeI GCC'GGC
Cuts at: 1327 4903 1327
Size: 3576 3148
NarI GG'CG_CC
Cuts at: 4153 4401 4153
Size: 248 6476
NcoI C'CATG_G
Cuts at: 125 1955 2697 3314 125
Size: 1830 742 617 3535
Fragments arranged by size:
3535 1830 742 617
NdeI CA'TA_TG
Cuts at: 3470 3470
Size: 6724
NgoMIV G'CCGG_C
Cuts at: 1325 4901 1325
Size: 3576 3148
NruI TCG'CGA
Cuts at: 5085 5085
Size: 6724
NspI r_CATG'y
Cuts at: 3341 3775 4275 5184 3341
Size: 434 500 909 4881
Fragments arranged by size:
4881 909 500 434
PciI A'CATG_T
Cuts at: 3337 5180 3337
Size: 1843 4881
PflMI CCAn_nnn'nTGG
Cuts at: 2862 2862
Size: 6724
PfoI T'CCnGG_A
Cuts at: 760 1049 760
Size: 289 6435
PmeI GTTT'AAAC
Cuts at: 3721 3721
Size: 6724
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1126 1158 5863 5895 1126
Size: 32 4705 32 1955
Fragments arranged by size:
4705 1955 32 32
PpuMI rG'GwC_Cy
Cuts at: 219 219
Size: 6724
PshAI GACnn'nnGTC
Cuts at: 3281 3281
Size: 6724
PspOMI G'GGCC_C
Cuts at: 139 1808 2962 139
Size: 1669 1154 3901
Fragments arranged by size:
3901 1669 1154
PstI C_TGCA'G
Cuts at: 239 826 1265 3769 4454 239
Size: 587 439 2504 685 2509
Fragments arranged by size:
2509 2504 685 587 439
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6724
PvuII CAG'CTG
Cuts at: 842 1291 1828 3681 4147 4507 842
Size: 449 537 1853 466 360 3059
Fragments arranged by size:
3059 1853 537 466 449 360
RsrII CG'GwC_CG
Cuts at: 4917 4917
Size: 6724
SacI G_AGCT'C
Cuts at: 3736 3736
Size: 6724
SalI G'TCGA_C
Cuts at: 596 3245 3759 596
Size: 2649 514 3561
Fragments arranged by size:
3561 2649 514
SapI GCTCTTCn'nnn_
Cuts at: 2260 4745 4955 2260
Size: 2485 210 4029
Fragments arranged by size:
4029 2485 210
SbfI CC_TGCA'GG
Cuts at: 3769 3769
Size: 6724
ScaI AGT'ACT
Cuts at: 1120 6241 1120
Size: 5121 1603
SexAI A'CCwGG_T
Cuts at: 875 875
Size: 6724
SfcI C'TryA_G
Cuts at: 33 235 822 1261 2132 2483 3698 3765 4450
Size: 202 587 439 871 351 1215 67 685
Cuts at: 4450 5445 5636 33
Size: 995 191 1121
Fragments arranged by size:
1215 1121 995 871 685 587 439 351
202 191 67
SfiI GGCCn_nnn'nGGCC
Cuts at: 1087 1087
Size: 6724
SfoI GGC'GCC
Cuts at: 4154 4402 4154
Size: 248 6476
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6724
SmaI CCC'GGG
Cuts at: 914 3744 914
Size: 2830 3894
SmlI C'TyrA_G
Cuts at: 1567 2184 3877 4792 5286 5548 1567
Size: 617 1693 915 494 262 2743
Fragments arranged by size:
2743 1693 915 617 494 262
SnaBI TAC'GTA
Cuts at: 3714 3714
Size: 6724
SpeI A'CTAG_T
Cuts at: 67 192 67
Size: 125 6599
SphI G_CATG'C
Cuts at: 3775 4275 3775
Size: 500 6224
SspI AAT'ATT
Cuts at: 1394 1394
Size: 6724
StuI AGG'CCT
Cuts at: 2054 2054
Size: 6724
StyI C'CwwG_G
Cuts at: 125 167 185 1091 1955 2420 2697 2856 3314
Size: 42 18 906 864 465 277 159 458
Cuts at: 3314 3856 4210 125
Size: 542 354 2639
Fragments arranged by size:
2639 906 864 542 465 458 354 277
159 42 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 118 559 1782 4305 4438 4897 5108 118
Size: 441 1223 2523 133 459 211 1734
Fragments arranged by size:
2523 1734 1223 459 441 211 133
TatI w'GTAC_w
Cuts at: 155 1118 3040 6239 155
Size: 963 1922 3199 640
Fragments arranged by size:
3199 1922 963 640
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1014 1046 1618 1650 2265 2297 6241 6273 1014
Size: 32 572 32 615 32 3944 32 1465
Fragments arranged by size:
3944 1465 615 572 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 325 1780 4519 325
Size: 1455 2739 2530
Fragments arranged by size:
2739 2530 1455
XbaI T'CTAG_A
Cuts at: 2601 3753 2601
Size: 1152 5572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 839 1395 1776 1915 4217 839
Size: 556 381 139 2302 3346
Fragments arranged by size:
3346 2302 556 381 139
XmaI C'CCGG_G
Cuts at: 912 3742 912
Size: 2830 3894
XmnI GAAnn'nnTTC
Cuts at: 3641 3641
Size: 6724
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
EcoNI EcoRV FspAI HpaI MfeI NheI NotI NsiI
PacI PasI PmlI PsiI PspXI PsrI SacII SanDI
SgrAI SrfI SwaI XhoI ZraI