Restriction map for
pF1KSDA0652
(Circular) (Six-base) MAPSORT of: pF1KSDA0652.seq Check: 1000 from: 1 to: 4690
pF1KSDA0652 4690 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 715 1726 715
Size: 1011 3679
Acc65I G'GTAC_C
Cuts at: 1053 1084 1704 1053
Size: 31 620 4039
Fragments arranged by size:
4039 620 31
AclI AA'CG_TT
Cuts at: 4530 4530
Size: 4690
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 1613 2513 2945 3694 3962 520
Size: 1093 900 432 749 268 1248
Fragments arranged by size:
1248 1093 900 749 432 268
AflII C'TTAA_G
Cuts at: 382 382
Size: 4690
AflIII A'CryG_T
Cuts at: 2040 3146 4123 2040
Size: 1106 977 2607
Fragments arranged by size:
2607 1106 977
AgeI A'CCGG_T
Cuts at: 3078 3078
Size: 4690
AleI CACnn'nnGTG
Cuts at: 661 661
Size: 4690
AlwNI CAG_nnn'CTG
Cuts at: 1381 1993 3562 1381
Size: 612 1569 2509
Fragments arranged by size:
2509 1569 612
ApaLI G'TGCA_C
Cuts at: 3460 3460
Size: 4690
ApoI r'AATT_y
Cuts at: 178 1326 1692 4043 4299 178
Size: 1148 366 2351 256 569
Fragments arranged by size:
2351 1148 569 366 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4690
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4690
AvaI C'yCGr_G
Cuts at: 1708 1708
Size: 4690
BamHI G'GATC_C
Cuts at: 1713 1713
Size: 4690
BanI G'GyrC_C
Cuts at: 655 667 1053 1084 1704 2118 2366 2401 655
Size: 12 386 31 620 414 248 35 2944
Fragments arranged by size:
2944 620 414 386 248 35 31 12
BanII G_rGCy'C
Cuts at: 1702 2732 1702
Size: 1030 3660
BbeI G_GCGC'C
Cuts at: 2122 2370 2122
Size: 248 4442
BbsI GAAGACnn'nnnn_
Cuts at: 869 1601 869
Size: 732 3958
BbvCI CC'TCA_GC
Cuts at: 1547 1547
Size: 4690
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1636 1670 1636
Size: 34 4656
BciVI GTATCCnnnnn_n'
Cuts at: 1161 1335 2586 3349 4690 1161
Size: 174 1251 763 1341 1161
Fragments arranged by size:
1341 1251 1161 763 174
BclI T'GATC_A
Cuts at: 762 2208 762
Size: 1446 3244
BglII A'GATC_T
Cuts at: 2203 4242 2203
Size: 2039 2651
BlpI GC'TnA_GC
Cuts at: 1552 1800 1552
Size: 248 4442
Bme1580I G_kGCm'C
Cuts at: 658 670 2313 2406 3464 658
Size: 12 1643 93 1058 1884
Fragments arranged by size:
1884 1643 1058 93 12
BmrI ACTGGGnnnn_n'
Cuts at: 1900 1981 2316 4462 1900
Size: 81 335 2146 2128
Fragments arranged by size:
2146 2128 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3055 3055
Size: 4690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 531 860 1140 1349 1357 1378 1404 531
Size: 329 280 209 8 21 26 3817
Fragments arranged by size:
3817 329 280 209 26 21 8
Bpu10I CC'TnA_GC
Cuts at: 615 1547 615
Size: 932 3758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 854 903 1864 2743 3237 3535 175
Size: 679 49 961 879 494 298 1330
Fragments arranged by size:
1330 961 879 679 494 298 49
BsaI GGTCTCn'nnnn_
Cuts at: 1219 4394 1219
Size: 3175 1515
BsaAI yAC'GTr
Cuts at: 1680 2041 1680
Size: 361 4329
BsaBI GATnn'nnATC
Cuts at: 6 1346 2202 4241 6
Size: 1340 856 2039 455
Fragments arranged by size:
2039 1340 856 455
BsaHI Gr'CG_yC
Cuts at: 2119 2367 3071 4567 2119
Size: 248 704 1496 2242
Fragments arranged by size:
2242 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1877 2398 3078 3352 3499 3910 1877
Size: 521 680 274 147 411 2657
Fragments arranged by size:
2657 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1371 1401 1371
Size: 30 4660
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 783 1379 783
Size: 596 4094
BseYI C'CCAG_C
Cuts at: 787 911 2113 2871 3450 3946 787
Size: 124 1202 758 579 496 1531
Fragments arranged by size:
1531 1202 758 579 496 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1101 1390 1101
Size: 289 4401
BsiEI CG_ry'CG
Cuts at: 104 2230 2276 3486 104
Size: 2126 46 1210 1308
Fragments arranged by size:
2126 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1581 1702 2480 2670 3464 1581
Size: 121 778 190 794 2807
Fragments arranged by size:
2807 794 778 190 121
BsmI GAATG_Cn'
Cuts at: 706 706
Size: 4690
Bsp1286I G_dGCh'C
Cuts at: 658 670 1581 1702 2313 2406 2480 2670 2732
Size: 12 911 121 611 93 74 190 62
Cuts at: 2732 3464 658
Size: 732 1884
Fragments arranged by size:
1884 911 732 611 190 121 93 74
62 12
BspEI T'CCGG_A
Cuts at: 3910 3910
Size: 4690
BspHI T'CATG_A
Cuts at: 3866 3866
Size: 4690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1123 1738 1952 2254 2635 1123
Size: 615 214 302 381 3178
Fragments arranged by size:
3178 615 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2980 3034 4520 4685 50
Size: 2930 54 1486 165 55
Fragments arranged by size:
2930 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 490 2600 490
Size: 2110 2580
BsrFI r'CCGG_y
Cuts at: 2686 2867 3078 2686
Size: 181 211 4298
Fragments arranged by size:
4298 211 181
BsrGI T'GTAC_A
Cuts at: 476 476
Size: 4690
BssSI C'ACGA_G
Cuts at: 2959 3319 2959
Size: 360 4330
BstAPI GCAn_nnn'nTGC
Cuts at: 2065 2065
Size: 4690
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4690
BstEII G'GTnAC_C
Cuts at: 1278 1278
Size: 4690
BstXI CCAn_nnnn'nTGG
Cuts at: 369 411 1329 369
Size: 42 918 3730
Fragments arranged by size:
3730 918 42
BstYI r'GATC_y
Cuts at: 1 1713 2180 2203 2538 2784 3798 3884 3896
Size: 1712 467 23 335 246 1014 86 12
Cuts at: 3896 4242 1
Size: 346 449
Fragments arranged by size:
1712 1014 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 404 1080 1586 125
Size: 279 676 506 3229
Fragments arranged by size:
3229 676 506 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2638 2819 2982 2638
Size: 181 163 4346
Fragments arranged by size:
4346 181 163
BtsI GCAGTG_nn'
Cuts at: 1197 1440 1931 1197
Size: 243 491 3956
Fragments arranged by size:
3956 491 243
DraI TTT'AAA
Cuts at: 1473 1687 1473
Size: 214 4476
DraIII CAC_nnn'GTG
Cuts at: 771 771
Size: 4690
DrdI GACnn_nn'nnGTC
Cuts at: 2394 3254 2394
Size: 860 3830
EaeI y'GGCC_r
Cuts at: 2273 2447 2838 2865 4212 2273
Size: 174 391 27 1347 2751
Fragments arranged by size:
2751 1347 391 174 27
EagI C'GGCC_G
Cuts at: 2273 2273
Size: 4690
EarI CTCTTCn'nnn_
Cuts at: 2711 2921 2711
Size: 210 4480
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2896 3208 3354 4500 2896
Size: 312 146 1146 3086
Fragments arranged by size:
3086 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1700 1700
Size: 4690
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 531 860 1140 1349 1357 1378 1404 1613
Size: 11 329 280 209 8 21 26 209
Cuts at: 1613 2513 2945 3694 3962 520
Size: 900 432 749 268 1248
Fragments arranged by size:
1248 900 749 432 329 280 268 209
209 26 21 11 8
EcoO109I rG'GnC_Cy
Cuts at: 159 734 1517 1827 159
Size: 575 783 310 3022
Fragments arranged by size:
3022 783 575 310
EcoRI G'AATT_C
Cuts at: 1692 1692
Size: 4690
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2707 2707
Size: 4690
FspI TGC'GCA
Cuts at: 2469 2469
Size: 4690
HaeII r_GCGC'y
Cuts at: 2122 2370 3394 4373 2122
Size: 248 1024 979 2439
Fragments arranged by size:
2439 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 225 257 1369 1371 1401 1403 1509 1541 1588
Size: 32 1112 2 30 2 106 32 47
Cuts at: 1588 1620 2530 2560 2562 2592 225
Size: 32 910 30 2 30 2323
Fragments arranged by size:
2323 1112 910 106 47 32 32 32
30 30 30 2 2 2
HincII GTy'rAC
Cuts at: 1260 1727 1260
Size: 467 4223
KasI G'GCGC_C
Cuts at: 2118 2366 2118
Size: 248 4442
KpnI G_GTAC'C
Cuts at: 1057 1088 1708 1057
Size: 31 620 4039
Fragments arranged by size:
4039 620 31
MluI A'CGCG_T
Cuts at: 4123 4123
Size: 4690
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 283 939 3361 3545 283
Size: 656 2422 184 1428
Fragments arranged by size:
2422 1428 656 184
MscI TGG'CCA
Cuts at: 2449 2449
Size: 4690
MslI CAynn'nnrTG
Cuts at: 367 566 661 1412 2677 2804 367
Size: 199 95 751 1265 127 2253
Fragments arranged by size:
2253 1265 751 199 127 95
MspA1I CmG'CkG
Cuts at: 342 606 967 1026 1424 1551 1799 2113 2473
Size: 264 361 59 398 127 248 314 360
Cuts at: 2473 3488 3733 342
Size: 1015 245 1299
Fragments arranged by size:
1299 1015 398 361 360 314 264 248
245 127 59
NaeI GCC'GGC
Cuts at: 2869 2869
Size: 4690
NarI GG'CG_CC
Cuts at: 2119 2367 2119
Size: 248 4442
NcoI C'CATG_G
Cuts at: 125 404 1080 1586 125
Size: 279 676 506 3229
Fragments arranged by size:
3229 676 506 279
NgoMIV G'CCGG_C
Cuts at: 2867 2867
Size: 4690
NruI TCG'CGA
Cuts at: 3051 3051
Size: 4690
NspI r_CATG'y
Cuts at: 1741 2241 3150 1741
Size: 500 909 3281
Fragments arranged by size:
3281 909 500
PasI CC'CwG_GG
Cuts at: 951 951
Size: 4690
PciI A'CATG_T
Cuts at: 3146 3146
Size: 4690
PflMI CCAn_nnn'nTGG
Cuts at: 1029 1029
Size: 4690
PfoI T'CCnGG_A
Cuts at: 547 547
Size: 4690
PmeI GTTT'AAAC
Cuts at: 1687 1687
Size: 4690
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3829 3861 3829
Size: 32 4658
PpuMI rG'GwC_Cy
Cuts at: 159 734 1517 159
Size: 575 783 3332
Fragments arranged by size:
3332 783 575
PstI C_TGCA'G
Cuts at: 351 1676 1735 2420 351
Size: 1325 59 685 2621
Fragments arranged by size:
2621 1325 685 59
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4690
PvuII CAG'CTG
Cuts at: 342 606 967 1026 1551 2113 2473 342
Size: 264 361 59 525 562 360 2559
Fragments arranged by size:
2559 562 525 361 360 264 59
RsrII CG'GwC_CG
Cuts at: 2883 2883
Size: 4690
SacI G_AGCT'C
Cuts at: 1702 1702
Size: 4690
SalI G'TCGA_C
Cuts at: 1725 1725
Size: 4690
SapI GCTCTTCn'nnn_
Cuts at: 2711 2921 2711
Size: 210 4480
SbfI CC_TGCA'GG
Cuts at: 1735 1735
Size: 4690
ScaI AGT'ACT
Cuts at: 4207 4207
Size: 4690
SexAI A'CCwGG_T
Cuts at: 1274 1274
Size: 4690
SfcI C'TryA_G
Cuts at: 33 347 537 857 1672 1731 2416 3411 3602
Size: 314 190 320 815 59 685 995 191
Cuts at: 3602 33
Size: 1121
Fragments arranged by size:
1121 995 815 685 320 314 191 190
59
SfoI GGC'GCC
Cuts at: 2120 2368 2120
Size: 248 4442
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4690
SmaI CCC'GGG
Cuts at: 1710 1710
Size: 4690
SmlI C'TyrA_G
Cuts at: 190 382 869 918 1843 2758 3252 3514 190
Size: 192 487 49 925 915 494 262 1366
Fragments arranged by size:
1366 925 915 494 487 262 192 49
SnaBI TAC'GTA
Cuts at: 1680 1680
Size: 4690
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4690
SphI G_CATG'C
Cuts at: 1741 2241 1741
Size: 500 4190
StyI C'CwwG_G
Cuts at: 125 404 1080 1586 1822 2176 125
Size: 279 676 506 236 354 2639
Fragments arranged by size:
2639 676 506 354 279 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2271 2404 2863 3074 2271
Size: 133 459 211 3887
Fragments arranged by size:
3887 459 211 133
TatI w'GTAC_w
Cuts at: 476 4205 476
Size: 3729 961
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 406 438 1032 1064 4207 4239 406
Size: 32 594 32 3143 32 857
Fragments arranged by size:
3143 857 594 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2485 2485
Size: 4690
XbaI T'CTAG_A
Cuts at: 1719 1719
Size: 4690
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 220 1078 2183 220
Size: 858 1105 2727
Fragments arranged by size:
2727 1105 858
XmaI C'CCGG_G
Cuts at: 1708 1708
Size: 4690
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsiWI BsmBI
BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NsiI
PacI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SrfI SspI StuI SwaI XhoI
XmnI ZraI