Restriction map for
pF1KSDA0654
(Circular) (Six-base) MAPSORT of: pF1KSDA0654.seq Check: 9255 from: 1 to: 6721
pF1KSDA0654 6721 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 3144 3757 3144
Size: 613 6108
Acc65I G'GTAC_C
Cuts at: 3735 3735
Size: 6721
AclI AA'CG_TT
Cuts at: 6561 6561
Size: 6721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 1525 1927 3310 4544 4976 5725 5993 463
Size: 1062 402 1383 1234 432 749 268 1191
Fragments arranged by size:
1383 1234 1191 1062 749 432 402 268
AfeI AGC'GCT
Cuts at: 1057 1456 1057
Size: 399 6322
AflIII A'CryG_T
Cuts at: 2882 4071 5177 6154 2882
Size: 1189 1106 977 3449
Fragments arranged by size:
3449 1189 1106 977
AgeI A'CCGG_T
Cuts at: 5109 5109
Size: 6721
AleI CACnn'nnGTG
Cuts at: 2653 2653
Size: 6721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 373 803 2806 373
Size: 430 2003 4288
Fragments arranged by size:
4288 2003 430
AlwNI CAG_nnn'CTG
Cuts at: 414 1206 1218 1929 2081 2964 3423 4024 5593
Size: 792 12 711 152 883 459 601 1569
Cuts at: 5593 414
Size: 1542
Fragments arranged by size:
1569 1542 883 792 711 601 459 152
12
ApaI G_GGCC'C
Cuts at: 187 1374 2648 187
Size: 1187 1274 4260
Fragments arranged by size:
4260 1274 1187
ApaLI G'TGCA_C
Cuts at: 5491 5491
Size: 6721
ApoI r'AATT_y
Cuts at: 1305 3295 3433 3723 6074 6330 1305
Size: 1990 138 290 2351 256 1696
Fragments arranged by size:
2351 1990 1696 290 256 138
AseI AT'TA_AT
Cuts at: 20 20
Size: 6721
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6721
AvaI C'yCGr_G
Cuts at: 552 676 1240 2932 3671 3739 552
Size: 124 564 1692 739 68 3534
Fragments arranged by size:
3534 1692 739 564 124 68
BamHI G'GATC_C
Cuts at: 1716 3744 1716
Size: 2028 4693
BanI G'GyrC_C
Cuts at: 937 1686 1705 2163 2188 2371 2728 3735 4149
Size: 749 19 458 25 183 357 1007 414
Cuts at: 4149 4397 4432 937
Size: 248 35 3226
Fragments arranged by size:
3226 1007 749 458 414 357 248 183
35 25 19
BanII G_rGCy'C
Cuts at: 175 187 314 1374 1673 1784 1819 2232 2648
Size: 12 127 1060 299 111 35 413 416
Cuts at: 2648 3101 3609 3616 3626 3733 4763 175
Size: 453 508 7 10 107 1030 2133
Fragments arranged by size:
2133 1060 1030 508 453 416 413 299
127 111 107 35 12 10 7
BbeI G_GCGC'C
Cuts at: 4153 4401 4153
Size: 248 6473
BbvCI CC'TCA_GC
Cuts at: 2847 2847
Size: 6721
BciVI GTATCCnnnnn_n'
Cuts at: 4617 5380 6721 4617
Size: 763 1341 4617
Fragments arranged by size:
4617 1341 763
BclI T'GATC_A
Cuts at: 4239 4239
Size: 6721
BglI GCCn_nnn'nGGC
Cuts at: 170 2618 2710 2737 170
Size: 2448 92 27 4154
Fragments arranged by size:
4154 2448 92 27
BglII A'GATC_T
Cuts at: 4234 6273 4234
Size: 2039 4682
BlpI GC'TnA_GC
Cuts at: 346 1438 3831 346
Size: 1092 2393 3236
Fragments arranged by size:
3236 2393 1092
Bme1580I G_kGCm'C
Cuts at: 187 940 1374 1665 1710 2191 2374 2648 3336
Size: 753 434 291 45 481 183 274 688
Cuts at: 3336 4344 4437 5495 187
Size: 1008 93 1058 1413
Fragments arranged by size:
1413 1058 1008 753 688 481 434 291
274 183 93 45
BmgBI CAC'GTC
Cuts at: 1085 1920 3237 1085
Size: 835 1317 4569
Fragments arranged by size:
4569 1317 835
BmrI ACTGGGnnnn_n'
Cuts at: 540 1898 3931 4012 4347 6493 540
Size: 1358 2033 81 335 2146 768
Fragments arranged by size:
2146 2033 1358 768 335 81
BmtI G_CTAG'C
Cuts at: 1130 2542 1130
Size: 1412 5309
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 345 1521 1800 2847 5086 345
Size: 1176 279 1047 2239 1980
Fragments arranged by size:
2239 1980 1176 1047 279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 274 436 505 679 859 871 943 1069
Size: 45 162 69 174 180 12 72 126
Cuts at: 1069 1132 1351 1513 1626 1687 1975 2047 2246
Size: 63 219 162 113 61 288 72 199
Cuts at: 2246 2620 2686 3217 3445 229
Size: 374 66 531 228 3505
Fragments arranged by size:
3505 531 374 288 228 219 199 180
174 162 162 126 113 72 72 69
66 63 61 45 12
Bpu10I CC'TnA_GC
Cuts at: 1063 1510 2847 1063
Size: 447 1337 4937
Fragments arranged by size:
4937 1337 447
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1456 2317 2785 3097 3895 4774 5268 5566
Size: 474 861 468 312 798 879 494 298
Cuts at: 5566 982
Size: 2137
Fragments arranged by size:
2137 879 861 798 494 474 468 312
298
BsaI GGTCTCn'nnnn_
Cuts at: 567 1225 1652 2835 2929 3346 6425 567
Size: 658 427 1183 94 417 3079 863
Fragments arranged by size:
3079 1183 863 658 427 417 94
BsaAI yAC'GTr
Cuts at: 3711 4072 3711
Size: 361 6360
BsaBI GATnn'nnATC
Cuts at: 6 1603 2776 4233 6272 6
Size: 1597 1173 1457 2039 455
Fragments arranged by size:
2039 1597 1457 1173 455
BsaHI Gr'CG_yC
Cuts at: 1097 1193 4150 4398 5102 6598 1097
Size: 96 2957 248 704 1496 1220
Fragments arranged by size:
2957 1496 1220 704 248 96
BsaWI w'CCGG_w
Cuts at: 3185 3692 3908 4429 5109 5383 5530 5941 3185
Size: 507 216 521 680 274 147 411 3965
Fragments arranged by size:
3965 680 521 507 411 274 216 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 916 946 1069 1099 1105 1126 1135 1156 2893
Size: 30 123 30 6 21 9 21 1737
Cuts at: 2893 2923 3433 3463 916
Size: 30 510 30 4174
Fragments arranged by size:
4174 1737 510 123 30 30 30 30
21 21 9 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 555 694 697 856 940 943 1045 1327 1510
Size: 139 3 159 84 3 102 282 183
Cuts at: 1510 1531 1792 1876 1879 2005 2035 2617 2850
Size: 21 261 84 3 126 30 582 233
Cuts at: 2850 2914 555
Size: 64 4362
Fragments arranged by size:
4362 582 282 261 233 183 159 139
126 102 84 84 64 30 21 3
3 3
BseYI C'CCAG_C
Cuts at: 180 954 1070 1337 2134 2951 3013 3282 4144
Size: 774 116 267 797 817 62 269 862
Cuts at: 4144 4902 5481 5977 180
Size: 758 579 496 924
Fragments arranged by size:
924 862 817 797 774 758 579 496
269 267 116 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1942 2371 2776 1075
Size: 867 429 405 5020
Fragments arranged by size:
5020 867 429 405
BsiEI CG_ry'CG
Cuts at: 104 4261 4307 5517 104
Size: 4157 46 1210 1308
Fragments arranged by size:
4157 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 314 584 611 677 1673 2232 3101 3733 4511
Size: 270 27 66 996 559 869 632 778
Cuts at: 4511 4701 5495 314
Size: 190 794 1540
Fragments arranged by size:
1540 996 869 794 778 632 559 270
190 66 27
BsmI GAATG_Cn'
Cuts at: 3408 3408
Size: 6721
BsmBI CGTCTCn'nnnn_
Cuts at: 250 1091 1414 2437 3227 250
Size: 841 323 1023 790 3744
Fragments arranged by size:
3744 1023 841 790 323
Bsp1286I G_dGCh'C
Cuts at: 175 187 314 584 611 677 940 1374 1665
Size: 12 127 270 27 66 263 434 291
Cuts at: 1665 1673 1710 1784 1819 2191 2232 2374 2648
Size: 8 37 74 35 372 41 142 274
Cuts at: 2648 3101 3336 3609 3616 3626 3733 4344 4437
Size: 453 235 273 7 10 107 611 93
Cuts at: 4437 4511 4701 4763 5495 175
Size: 74 190 62 732 1401
Fragments arranged by size:
1401 732 611 453 434 372 291 274
273 270 263 235 190 142 127 107
93 74 74 66 62 41 37 35
27 12 10 8 7
BspEI T'CCGG_A
Cuts at: 3692 5941 3692
Size: 2249 4472
BspHI T'CATG_A
Cuts at: 5897 5897
Size: 6721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3769 3983 4285 4666 3769
Size: 214 302 381 5824
Fragments arranged by size:
5824 381 302 214
BsrBI CCG'CTC
Cuts at: 50 642 855 1035 1317 1590 1800 2285 2726
Size: 592 213 180 282 273 210 485 441
Cuts at: 2726 3201 3258 3320 3503 5011 5065 6551 6716
Size: 475 57 62 183 1508 54 1486 165
Cuts at: 6716 50
Size: 55
Fragments arranged by size:
1508 1486 592 485 475 441 282 273
213 210 183 180 165 62 57 55
54
BsrDI GCAATG_nn'
Cuts at: 4631 4631
Size: 6721
BsrFI r'CCGG_y
Cuts at: 164 821 2247 4717 4898 5109 164
Size: 657 1426 2470 181 211 1776
Fragments arranged by size:
2470 1776 1426 657 211 181
BssHII G'CGCG_C
Cuts at: 861 861
Size: 6721
BssSI C'ACGA_G
Cuts at: 319 505 2234 2896 2986 4990 5350 319
Size: 186 1729 662 90 2004 360 1690
Fragments arranged by size:
2004 1729 1690 662 360 186 90
BstAPI GCAn_nnn'nTGC
Cuts at: 1206 1218 2081 4096 1206
Size: 12 863 2015 3831
Fragments arranged by size:
3831 2015 863 12
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6721
BstXI CCAn_nnnn'nTGG
Cuts at: 798 3661 798
Size: 2863 3858
BstYI r'GATC_y
Cuts at: 1 1716 2759 2960 3227 3389 3744 4211 4234
Size: 1715 1043 201 267 162 355 467 23
Cuts at: 4234 4569 4815 5829 5915 5927 6273 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1715 1043 1014 467 449 355 346 335
267 246 201 162 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2213 3453 2213
Size: 1240 5481
BtgI C'CryG_G
Cuts at: 527 2356 2651 527
Size: 1829 295 4597
Fragments arranged by size:
4597 1829 295
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 336 4669 4850 5013 336
Size: 4333 181 163 2044
Fragments arranged by size:
4333 2044 181 163
BtsI GCAGTG_nn'
Cuts at: 514 677 1665 1740 2320 3334 3962 514
Size: 163 988 75 580 1014 628 3273
Fragments arranged by size:
3273 1014 988 628 580 163 75
DraI TTT'AAA
Cuts at: 3718 3718
Size: 6721
DraIII CAC_nnn'GTG
Cuts at: 3138 3138
Size: 6721
DrdI GACnn_nn'nnGTC
Cuts at: 4425 5285 4425
Size: 860 5861
EaeI y'GGCC_r
Cuts at: 162 315 1175 1577 1931 2142 2249 2519 2699
Size: 153 860 402 354 211 107 270 180
Cuts at: 2699 2738 2918 4304 4478 4869 4896 6243 162
Size: 39 180 1386 174 391 27 1347 640
Fragments arranged by size:
1386 1347 860 640 402 391 354 270
211 180 180 174 153 107 39 27
EagI C'GGCC_G
Cuts at: 4304 4304
Size: 6721
EarI CTCTTCn'nnn_
Cuts at: 422 607 749 1151 1156 1340 2401 2490 4742
Size: 185 142 402 5 184 1061 89 2252
Cuts at: 4742 4952 422
Size: 210 2191
Fragments arranged by size:
2252 2191 1061 402 210 185 184 142
89 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 463 3531 4927 5239 5385 6531 463
Size: 3068 1396 312 146 1146 653
Fragments arranged by size:
3068 1396 1146 653 312 146
EcoICRI GAG'CTC
Cuts at: 312 1671 2230 3099 3731 312
Size: 1359 559 869 632 3302
Fragments arranged by size:
3302 1359 869 632 559
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 274 436 463 505 679 859 871 943
Size: 45 162 27 42 174 180 12 72
Cuts at: 943 1069 1132 1351 1513 1525 1626 1687 1927
Size: 126 63 219 162 12 101 61 240
Cuts at: 1927 1975 2047 2246 2620 2686 3217 3310 3445
Size: 48 72 199 374 66 531 93 135
Cuts at: 3445 4544 4976 5725 5993 229
Size: 1099 432 749 268 957
Fragments arranged by size:
1099 957 749 531 432 374 268 240
219 199 180 174 162 162 135 126
101 93 72 72 66 63 61 48
45 42 27 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1000 1513 1641 2602 1000
Size: 513 128 961 5119
Fragments arranged by size:
5119 961 513 128
EcoO109I rG'GnC_Cy
Cuts at: 618 802 846 2333 2350 2464 2619 3192 3537
Size: 184 44 1487 17 114 155 573 345
Cuts at: 3537 3858 618
Size: 321 3481
Fragments arranged by size:
3481 1487 573 345 321 184 155 114
44 17
EcoRI G'AATT_C
Cuts at: 3433 3723 3433
Size: 290 6431
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 618 4738 618
Size: 4120 2601
FseI GG_CCGG'CC
Cuts at: 2251 2251
Size: 6721
FspI TGC'GCA
Cuts at: 520 4500 520
Size: 3980 2741
HaeII r_GCGC'y
Cuts at: 181 216 249 342 540 658 891 1009 1059
Size: 35 33 93 198 118 233 118 50
Cuts at: 1059 1070 1279 1458 1492 3488 4153 4401 5425
Size: 11 209 179 34 1996 665 248 1024
Cuts at: 5425 6404 181
Size: 979 498
Fragments arranged by size:
1996 1024 979 665 498 248 233 209
198 179 118 118 93 50 35 34
33 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1069 1101 1882 1914 2893 2925 4561 4591 4593
Size: 32 781 32 979 32 1636 30 2
Cuts at: 4593 4623 1069
Size: 30 3167
Fragments arranged by size:
3167 1636 979 781 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3758 3758
Size: 6721
HindIII A'AGCT_T
Cuts at: 1537 1537
Size: 6721
KasI G'GCGC_C
Cuts at: 4149 4397 4149
Size: 248 6473
KpnI G_GTAC'C
Cuts at: 3739 3739
Size: 6721
MluI A'CGCG_T
Cuts at: 6154 6154
Size: 6721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 842 1166 1472 3392 5392 5576 842
Size: 324 306 1920 2000 184 1987
Fragments arranged by size:
2000 1987 1920 324 306 184
MscI TGG'CCA
Cuts at: 1933 2144 2521 2740 4480 1933
Size: 211 377 219 1740 4174
Fragments arranged by size:
4174 1740 377 219 211
MslI CAynn'nnrTG
Cuts at: 526 2653 4708 4835 526
Size: 2127 2055 127 2412
Fragments arranged by size:
2412 2127 2055 127
MspA1I CmG'CkG
Cuts at: 297 411 729 879 921 1017 1074 1173 1257
Size: 114 318 150 42 96 57 99 84
Cuts at: 1257 1329 1341 1368 1380 1614 1760 1953 2081
Size: 72 12 27 12 234 146 193 128
Cuts at: 2081 2790 2951 3565 3601 3830 4144 4504 5519
Size: 709 161 614 36 229 314 360 1015
Cuts at: 5519 5764 297
Size: 245 1254
Fragments arranged by size:
1254 1015 709 614 360 318 314 245
234 229 193 161 150 146 128 114
99 96 84 72 57 42 36 27
12 12
NaeI GCC'GGC
Cuts at: 166 2249 4900 166
Size: 2083 2651 1987
Fragments arranged by size:
2651 2083 1987
NarI GG'CG_CC
Cuts at: 4150 4398 4150
Size: 248 6473
NdeI CA'TA_TG
Cuts at: 2970 2970
Size: 6721
NgoMIV G'CCGG_C
Cuts at: 164 2247 4898 164
Size: 2083 2651 1987
Fragments arranged by size:
2651 2083 1987
NheI G'CTAG_C
Cuts at: 1126 2538 1126
Size: 1412 5309
NruI TCG'CGA
Cuts at: 5082 5082
Size: 6721
NspI r_CATG'y
Cuts at: 2685 3772 4272 5181 2685
Size: 1087 500 909 4225
Fragments arranged by size:
4225 1087 909 500
PasI CC'CwG_GG
Cuts at: 1837 3609 1837
Size: 1772 4949
PciI A'CATG_T
Cuts at: 5177 5177
Size: 6721
PflMI CCAn_nnn'nTGG
Cuts at: 1793 1837 2316 2826 1793
Size: 44 479 510 5688
Fragments arranged by size:
5688 510 479 44
PfoI T'CCnGG_A
Cuts at: 473 635 2597 2819 3527 473
Size: 162 1962 222 708 3667
Fragments arranged by size:
3667 1962 708 222 162
PmeI GTTT'AAAC
Cuts at: 3718 3718
Size: 6721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5860 5892 5860
Size: 32 6689
PpuMI rG'GwC_Cy
Cuts at: 802 2333 2350 2464 3192 3537 802
Size: 1531 17 114 728 345 3986
Fragments arranged by size:
3986 1531 728 345 114 17
PspOMI G'GGCC_C
Cuts at: 183 1370 2644 183
Size: 1187 1274 4260
Fragments arranged by size:
4260 1274 1187
PspXI vC'TCGA_Gb
Cuts at: 676 676
Size: 6721
PstI C_TGCA'G
Cuts at: 338 989 1211 1223 1367 2327 3389 3600 3644
Size: 651 222 12 144 960 1062 211 44
Cuts at: 3644 3766 4451 338
Size: 122 685 2608
Fragments arranged by size:
2608 1062 960 685 651 222 211 144
122 44 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6721
PvuII CAG'CTG
Cuts at: 297 411 729 879 921 1173 1257 1329 1341
Size: 114 318 150 42 252 84 72 12
Cuts at: 1341 1614 1953 2081 2951 3565 4144 4504 297
Size: 273 339 128 870 614 579 360 2514
Fragments arranged by size:
2514 870 614 579 360 339 318 273
252 150 128 114 84 72 42 12
RsrII CG'GwC_CG
Cuts at: 3690 4914 3690
Size: 1224 5497
SacI G_AGCT'C
Cuts at: 314 1673 2232 3101 3733 314
Size: 1359 559 869 632 3302
Fragments arranged by size:
3302 1359 869 632 559
SalI G'TCGA_C
Cuts at: 3756 3756
Size: 6721
SanDI GG'GwC_CC
Cuts at: 2333 2350 2464 2333
Size: 17 114 6590
Fragments arranged by size:
6590 114 17
SapI GCTCTTCn'nnn_
Cuts at: 749 1156 2401 2490 4742 4952 749
Size: 407 1245 89 2252 210 2518
Fragments arranged by size:
2518 2252 1245 407 210 89
SbfI CC_TGCA'GG
Cuts at: 3766 3766
Size: 6721
ScaI AGT'ACT
Cuts at: 6238 6238
Size: 6721
SfcI C'TryA_G
Cuts at: 33 287 334 985 1207 1219 1363 2323 3385
Size: 254 47 651 222 12 144 960 1062
Cuts at: 3385 3596 3640 3762 4447 5442 5633 33
Size: 211 44 122 685 995 191 1121
Fragments arranged by size:
1121 1062 995 960 685 651 254 222
211 191 144 122 47 44 12
SfoI GGC'GCC
Cuts at: 4151 4399 4151
Size: 248 6473
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6721
SmaI CCC'GGG
Cuts at: 554 3673 3741 554
Size: 3119 68 3534
Fragments arranged by size:
3534 3119 68
SmlI C'TyrA_G
Cuts at: 676 997 1471 2296 2800 3112 3874 4789 5283
Size: 321 474 825 504 312 762 915 494
Cuts at: 5283 5545 676
Size: 262 1852
Fragments arranged by size:
1852 915 825 762 504 494 474 321
312 262
SnaBI TAC'GTA
Cuts at: 3711 3711
Size: 6721
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6721
SphI G_CATG'C
Cuts at: 2685 3772 4272 2685
Size: 1087 500 5134
Fragments arranged by size:
5134 1087 500
StuI AGG'CCT
Cuts at: 2314 2608 2314
Size: 294 6427
StyI C'CwwG_G
Cuts at: 1787 2315 2939 3853 4207 1787
Size: 528 624 914 354 4301
Fragments arranged by size:
4301 914 624 528 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1704 2289 2585 2664 2982 3261 3678 4302 4435
Size: 585 296 79 318 279 417 624 133
Cuts at: 4435 4894 5105 1704
Size: 459 211 3320
Fragments arranged by size:
3320 624 585 459 417 318 296 279
211 133 79
TatI w'GTAC_w
Cuts at: 6236 6236
Size: 6721
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1817 1849 6238 6270 1817
Size: 32 4389 32 2268
Fragments arranged by size:
4389 2268 32 32
Tth111I GACn'n_nGTC
Cuts at: 4516 4516
Size: 6721
XbaI T'CTAG_A
Cuts at: 3750 3750
Size: 6721
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 553 1107 2049 2529 2823 4214 553
Size: 554 942 480 294 1391 3060
Fragments arranged by size:
3060 1391 942 554 480 294
XhoI C'TCGA_G
Cuts at: 676 676
Size: 6721
XmaI C'CCGG_G
Cuts at: 552 3671 3739 552
Size: 3119 68 3534
Fragments arranged by size:
3534 3119 68
XmnI GAAnn'nnTTC
Cuts at: 3433 3523 3433
Size: 90 6631
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NheI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI AscI AvrII BaeI
BbsI BcgI BfrBI BsiWI BsrGI BstEII BstZ17I ClaI
CspCI EcoRV FspAI HpaI MfeI NcoI NotI NsiI
PacI PmlI PshAI PsiI PsrI SacII SexAI SfiI
SgrAI SrfI SspI SwaI ZraI