Restriction map for
pF1KSDA0658
(Circular) (Six-base) MAPSORT of: pF1KSDA0658.seq Check: 5222 from: 1 to: 4918
pF1KSDA0658 4918 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1677 1954 1677
Size: 277 4641
Acc65I G'GTAC_C
Cuts at: 1932 1932
Size: 4918
AclI AA'CG_TT
Cuts at: 4758 4758
Size: 4918
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 809 2741 3173 3922 4190 334
Size: 475 1932 432 749 268 1062
Fragments arranged by size:
1932 1062 749 475 432 268
AfeI AGC'GCT
Cuts at: 637 637
Size: 4918
AflIII A'CryG_T
Cuts at: 428 2268 3374 4351 428
Size: 1840 1106 977 995
Fragments arranged by size:
1840 1106 995 977
AgeI A'CCGG_T
Cuts at: 3306 3306
Size: 4918
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 235 682 1575 235
Size: 447 893 3578
Fragments arranged by size:
3578 893 447
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 763 795 892 924 763
Size: 32 97 32 4757
Fragments arranged by size:
4757 97 32 32
AlwNI CAG_nnn'CTG
Cuts at: 357 2221 3790 357
Size: 1864 1569 1485
Fragments arranged by size:
1864 1569 1485
ApaI G_GGCC'C
Cuts at: 1860 1860
Size: 4918
ApaLI G'TGCA_C
Cuts at: 199 3688 199
Size: 3489 1429
ApoI r'AATT_y
Cuts at: 564 1920 4271 4527 564
Size: 1356 2351 256 955
Fragments arranged by size:
2351 1356 955 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4918
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4918
AvaI C'yCGr_G
Cuts at: 1737 1859 1936 1737
Size: 122 77 4719
Fragments arranged by size:
4719 122 77
BamHI G'GATC_C
Cuts at: 1238 1941 1238
Size: 703 4215
BanI G'GyrC_C
Cuts at: 165 1932 2346 2594 2629 165
Size: 1767 414 248 35 2454
Fragments arranged by size:
2454 1767 414 248 35
BanII G_rGCy'C
Cuts at: 1523 1733 1860 1930 2960 1523
Size: 210 127 70 1030 3481
Fragments arranged by size:
3481 1030 210 127 70
BbeI G_GCGC'C
Cuts at: 169 2350 2598 169
Size: 2181 248 2489
Fragments arranged by size:
2489 2181 248
BbsI GAAGACnn'nnnn_
Cuts at: 1712 1712
Size: 4918
BbvCI CC'TCA_GC
Cuts at: 934 1175 934
Size: 241 4677
BciVI GTATCCnnnnn_n'
Cuts at: 2814 3577 4918 2814
Size: 763 1341 2814
Fragments arranged by size:
2814 1341 763
BclI T'GATC_A
Cuts at: 2436 2436
Size: 4918
BglI GCCn_nnn'nGGC
Cuts at: 538 929 1171 1430 1748 1864 538
Size: 391 242 259 318 116 3592
Fragments arranged by size:
3592 391 318 259 242 116
BglII A'GATC_T
Cuts at: 2431 4470 2431
Size: 2039 2879
BlpI GC'TnA_GC
Cuts at: 2028 2028
Size: 4918
Bme1580I G_kGCm'C
Cuts at: 203 761 1860 2541 2634 3692 203
Size: 558 1099 681 93 1058 1429
Fragments arranged by size:
1429 1099 1058 681 558 93
BmgBI CAC'GTC
Cuts at: 429 429
Size: 4918
BmrI ACTGGGnnnn_n'
Cuts at: 1448 1778 2128 2209 2544 4690 1448
Size: 330 350 81 335 2146 1676
Fragments arranged by size:
2146 1676 350 335 330 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1752 3283 1752
Size: 1531 3387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 787
Size: 4918
Bpu10I CC'TnA_GC
Cuts at: 934 1175 934
Size: 241 4677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2092 2971 3465 3763 2092
Size: 879 494 298 3247
Fragments arranged by size:
3247 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 739 882 1600 4622 739
Size: 143 718 3022 1035
Fragments arranged by size:
3022 1035 718 143
BsaAI yAC'GTr
Cuts at: 1908 2269 1908
Size: 361 4557
BsaBI GATnn'nnATC
Cuts at: 6 2430 4469 6
Size: 2424 2039 455
Fragments arranged by size:
2424 2039 455
BsaHI Gr'CG_yC
Cuts at: 166 2347 2595 3299 4795 166
Size: 2181 248 704 1496 289
Fragments arranged by size:
2181 1496 704 289 248
BsaWI w'CCGG_w
Cuts at: 2105 2626 3306 3580 3727 4138 2105
Size: 521 680 274 147 411 2885
Fragments arranged by size:
2885 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 763 793 892 922 763
Size: 30 99 30 4759
Fragments arranged by size:
4759 99 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 303 306 784 833 303
Size: 3 478 49 4388
Fragments arranged by size:
4388 478 49 3
BseYI C'CCAG_C
Cuts at: 774 1301 1732 1791 2341 3099 3678 4174 774
Size: 527 431 59 550 758 579 496 1518
Fragments arranged by size:
1518 758 579 550 527 496 431 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1784 1784
Size: 4918
BsiEI CG_ry'CG
Cuts at: 104 254 2458 2504 3714 104
Size: 150 2204 46 1210 1308
Fragments arranged by size:
2204 1308 1210 150 46
BsiHKAI G_wGCw'C
Cuts at: 203 1930 2708 2898 3692 203
Size: 1727 778 190 794 1429
Fragments arranged by size:
1727 1429 794 778 190
BsmI GAATG_Cn'
Cuts at: 1533 1533
Size: 4918
Bsp1286I G_dGCh'C
Cuts at: 203 761 1523 1733 1860 1930 2541 2634 2708
Size: 558 762 210 127 70 611 93 74
Cuts at: 2708 2898 2960 3692 203
Size: 190 62 732 1429
Fragments arranged by size:
1429 762 732 611 558 210 190 127
93 74 70 62
BspEI T'CCGG_A
Cuts at: 4138 4138
Size: 4918
BspHI T'CATG_A
Cuts at: 521 1211 4094 521
Size: 690 2883 1345
Fragments arranged by size:
2883 1345 690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 953 1487 1966 2180 2482 2863 953
Size: 534 479 214 302 381 3008
Fragments arranged by size:
3008 534 479 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1310 3208 3262 4748 4913 50
Size: 1260 1898 54 1486 165 55
Fragments arranged by size:
1898 1486 1260 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2828 2828
Size: 4918
BsrFI r'CCGG_y
Cuts at: 1613 2914 3095 3306 1613
Size: 1301 181 211 3225
Fragments arranged by size:
3225 1301 211 181
BssHII G'CGCG_C
Cuts at: 265 265
Size: 4918
BssSI C'ACGA_G
Cuts at: 3187 3547 3187
Size: 360 4558
BstAPI GCAn_nnn'nTGC
Cuts at: 2293 2293
Size: 4918
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4918
BstEII G'GTnAC_C
Cuts at: 690 690
Size: 4918
BstXI CCAn_nnnn'nTGG
Cuts at: 809 809
Size: 4918
BstYI r'GATC_y
Cuts at: 1 725 1133 1238 1941 2408 2431 2766 3012
Size: 724 408 105 703 467 23 335 246
Cuts at: 3012 4026 4112 4124 4470 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 724 703 467 449 408 346 335
246 105 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1154 1154
Size: 4918
BtgI C'CryG_G
Cuts at: 125 298 1262 1281 1582 125
Size: 173 964 19 301 3461
Fragments arranged by size:
3461 964 301 173 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2866 3047 3210 2866
Size: 181 163 4574
Fragments arranged by size:
4574 181 163
BtsI GCAGTG_nn'
Cuts at: 1395 1413 1767 2159 1395
Size: 18 354 392 4154
Fragments arranged by size:
4154 392 354 18
DraI TTT'AAA
Cuts at: 1915 1915
Size: 4918
DraIII CAC_nnn'GTG
Cuts at: 1722 1837 1722
Size: 115 4803
DrdI GACnn_nn'nnGTC
Cuts at: 1294 2622 3482 1294
Size: 1328 860 2730
Fragments arranged by size:
2730 1328 860
EaeI y'GGCC_r
Cuts at: 251 530 917 1163 1437 2501 2675 3066 3093
Size: 279 387 246 274 1064 174 391 27
Cuts at: 3093 4440 251
Size: 1347 729
Fragments arranged by size:
1347 1064 729 391 387 279 274 246
174 27
EagI C'GGCC_G
Cuts at: 251 2501 251
Size: 2250 2668
EarI CTCTTCn'nnn_
Cuts at: 198 979 2939 3149 198
Size: 781 1960 210 1967
Fragments arranged by size:
1967 1960 781 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3124 3436 3582 4728 3124
Size: 312 146 1146 3314
Fragments arranged by size:
3314 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1928 1928
Size: 4918
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 787 809 2741 3173 3922 4190 334
Size: 453 22 1932 432 749 268 1062
Fragments arranged by size:
1932 1062 749 453 432 268 22
EcoO109I rG'GnC_Cy
Cuts at: 993 1158 1287 2055 993
Size: 165 129 768 3856
Fragments arranged by size:
3856 768 165 129
EcoRI G'AATT_C
Cuts at: 564 1920 564
Size: 1356 3562
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1206 2935 1206
Size: 1729 3189
FspI TGC'GCA
Cuts at: 2697 2697
Size: 4918
HaeII r_GCGC'y
Cuts at: 169 175 191 639 2350 2598 3622 4601 169
Size: 6 16 448 1711 248 1024 979 486
Fragments arranged by size:
1711 1024 979 486 448 248 16 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 175 207 816 848 2758 2788 2790 2820 175
Size: 32 609 32 1910 30 2 30 2273
Fragments arranged by size:
2273 1910 609 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1596 1955 1596
Size: 359 4559
KasI G'GCGC_C
Cuts at: 165 2346 2594 165
Size: 2181 248 2489
Fragments arranged by size:
2489 2181 248
KpnI G_GTAC'C
Cuts at: 1936 1936
Size: 4918
MluI A'CGCG_T
Cuts at: 4351 4351
Size: 4918
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3589 3773 3589
Size: 184 4734
MscI TGG'CCA
Cuts at: 532 919 1165 2677 532
Size: 387 246 1512 2773
Fragments arranged by size:
2773 1512 387 246
MslI CAynn'nnrTG
Cuts at: 526 1570 2905 3032 526
Size: 1044 1335 127 2412
Fragments arranged by size:
2412 1335 1044 127
MspA1I CmG'CkG
Cuts at: 154 805 1283 1301 1370 2027 2341 2701 3716
Size: 651 478 18 69 657 314 360 1015
Cuts at: 3716 3961 154
Size: 245 1111
Fragments arranged by size:
1111 1015 657 651 478 360 314 245
69 18
NaeI GCC'GGC
Cuts at: 1615 3097 1615
Size: 1482 3436
NarI GG'CG_CC
Cuts at: 166 2347 2595 166
Size: 2181 248 2489
Fragments arranged by size:
2489 2181 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4918
NgoMIV G'CCGG_C
Cuts at: 1613 3095 1613
Size: 1482 3436
NruI TCG'CGA
Cuts at: 3279 3279
Size: 4918
NspI r_CATG'y
Cuts at: 1261 1969 2469 3378 1261
Size: 708 500 909 2801
Fragments arranged by size:
2801 909 708 500
PasI CC'CwG_GG
Cuts at: 1139 1139
Size: 4918
PciI A'CATG_T
Cuts at: 3374 3374
Size: 4918
PflMI CCAn_nnn'nTGG
Cuts at: 705 1248 705
Size: 543 4375
PfoI T'CCnGG_A
Cuts at: 728 1337 728
Size: 609 4309
PmeI GTTT'AAAC
Cuts at: 1915 1915
Size: 4918
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 763 795 892 924 4057 4089 763
Size: 32 97 32 3133 32 1592
Fragments arranged by size:
3133 1592 97 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 993 1287 993
Size: 294 4624
PshAI GACnn'nnGTC
Cuts at: 1712 1712
Size: 4918
PspOMI G'GGCC_C
Cuts at: 1856 1856
Size: 4918
PspXI vC'TCGA_Gb
Cuts at: 1737 1737
Size: 4918
PstI C_TGCA'G
Cuts at: 718 1390 1963 2648 718
Size: 672 573 685 2988
Fragments arranged by size:
2988 685 672 573
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4918
PvuII CAG'CTG
Cuts at: 154 805 1301 1370 2341 2701 154
Size: 651 496 69 971 360 2371
Fragments arranged by size:
2371 971 651 496 360 69
RsrII CG'GwC_CG
Cuts at: 3111 3111
Size: 4918
SacI G_AGCT'C
Cuts at: 1930 1930
Size: 4918
SacII CC_GC'GG
Cuts at: 1284 1284
Size: 4918
SalI G'TCGA_C
Cuts at: 1953 1953
Size: 4918
SapI GCTCTTCn'nnn_
Cuts at: 2939 3149 2939
Size: 210 4708
SbfI CC_TGCA'GG
Cuts at: 1963 1963
Size: 4918
ScaI AGT'ACT
Cuts at: 4435 4435
Size: 4918
SexAI A'CCwGG_T
Cuts at: 1833 1833
Size: 4918
SfcI C'TryA_G
Cuts at: 33 714 1386 1959 2644 3639 3830 33
Size: 681 672 573 685 995 191 1121
Fragments arranged by size:
1121 995 685 681 672 573 191
SfoI GGC'GCC
Cuts at: 167 2348 2596 167
Size: 2181 248 2489
Fragments arranged by size:
2489 2181 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4918
SmaI CCC'GGG
Cuts at: 1861 1938 1861
Size: 77 4841
SmlI C'TyrA_G
Cuts at: 1737 2071 2986 3480 3742 1737
Size: 334 915 494 262 2913
Fragments arranged by size:
2913 915 494 334 262
SnaBI TAC'GTA
Cuts at: 1908 1908
Size: 4918
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4918
SphI G_CATG'C
Cuts at: 1261 1969 2469 1261
Size: 708 500 3710
Fragments arranged by size:
3710 708 500
StuI AGG'CCT
Cuts at: 868 932 868
Size: 64 4854
StyI C'CwwG_G
Cuts at: 125 436 533 1195 2050 2404 125
Size: 311 97 662 855 354 2639
Fragments arranged by size:
2639 855 662 354 311 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2499 2632 3091 3302 2499
Size: 133 459 211 4115
Fragments arranged by size:
4115 459 211 133
TatI w'GTAC_w
Cuts at: 4433 4433
Size: 4918
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4435 4467 4435
Size: 32 4886
Tth111I GACn'n_nGTC
Cuts at: 797 1673 2713 797
Size: 876 1040 3002
Fragments arranged by size:
3002 1040 876
XbaI T'CTAG_A
Cuts at: 1947 1947
Size: 4918
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 525 678 702 912 1809 2411 525
Size: 153 24 210 897 602 3032
Fragments arranged by size:
3032 897 602 210 153 24
XhoI C'TCGA_G
Cuts at: 1737 1737
Size: 4918
XmaI C'CCGG_G
Cuts at: 1859 1936 1859
Size: 77 4841
XmnI GAAnn'nnTTC
Cuts at: 1495 1495
Size: 4918
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AscI AvrII BaeI
BcgI BfrBI BmtI BsiWI BsmBI BsrGI BstZ17I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NsiI PacI PmlI PsiI PsrI
SanDI SfiI SgrAI SrfI SspI SwaI ZraI