Restriction map for
pF1KSDA0659
(Circular) (Six-base) MAPSORT of: pF1KSDA0659.seq Check: 3932 from: 1 to: 6263
pF1KSDA0659 6263 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2084 2084
Size: 6263
AccI GT'mk_AC
Cuts at: 2407 3301 2407
Size: 894 5369
Acc65I G'GTAC_C
Cuts at: 3279 3279
Size: 6263
AclI AA'CG_TT
Cuts at: 6103 6103
Size: 6263
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 4088 4520 5267 5535 4088
Size: 432 747 268 4816
Fragments arranged by size:
4816 747 432 268
AflIII A'CryG_T
Cuts at: 3615 4719 5696 3615
Size: 1104 977 4182
Fragments arranged by size:
4182 1104 977
AgeI A'CCGG_T
Cuts at: 4651 4651
Size: 6263
AhdI GACnn_n'nnGTC
Cuts at: 756 2484 2661 756
Size: 1728 177 4358
Fragments arranged by size:
4358 1728 177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 901 1989 2659 2953 901
Size: 1088 670 294 4211
Fragments arranged by size:
4211 1088 670 294
AlwNI CAG_nnn'CTG
Cuts at: 1749 2691 2856 3568 5135 1749
Size: 942 165 712 1567 2877
Fragments arranged by size:
2877 1567 942 712 165
ApaI G_GGCC'C
Cuts at: 2974 2974
Size: 6263
ApaLI G'TGCA_C
Cuts at: 5033 5033
Size: 6263
ApoI r'AATT_y
Cuts at: 969 2860 3267 5616 5872 969
Size: 1891 407 2349 256 1360
Fragments arranged by size:
2349 1891 1360 407 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6263
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6263
AvaI C'yCGr_G
Cuts at: 130 2608 2941 3075 3114 3283 130
Size: 2478 333 134 39 169 3110
Fragments arranged by size:
3110 2478 333 169 134 39
BamHI G'GATC_C
Cuts at: 3288 3288
Size: 6263
BanI G'GyrC_C
Cuts at: 674 1190 1388 1396 2448 2825 2937 3030 3279
Size: 516 198 8 1052 377 112 93 249
Cuts at: 3279 3693 3941 3976 674
Size: 414 248 35 2961
Fragments arranged by size:
2961 1052 516 414 377 249 248 198
112 93 35 8
BanII G_rGCy'C
Cuts at: 395 2000 2102 2173 2201 2542 2632 2950 2974
Size: 1605 102 71 28 341 90 318 24
Cuts at: 2974 3023 3125 3277 4307 395
Size: 49 102 152 1030 2351
Fragments arranged by size:
2351 1605 1030 341 318 152 102 102
90 71 49 28 24
BbeI G_GCGC'C
Cuts at: 1194 2829 3697 3945 1194
Size: 1635 868 248 3512
Fragments arranged by size:
3512 1635 868 248
BbsI GAAGACnn'nnnn_
Cuts at: 585 1160 2140 585
Size: 575 980 4708
Fragments arranged by size:
4708 980 575
BbvCI CC'TCA_GC
Cuts at: 2966 2966
Size: 6263
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 496 530 817 851 1680 1714 496
Size: 34 287 34 829 34 5045
Fragments arranged by size:
5045 829 287 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1324 1601 4161 4922 6263 1324
Size: 277 2560 761 1341 1324
Fragments arranged by size:
2560 1341 1324 761 277
BclI T'GATC_A
Cuts at: 1307 2045 3783 1307
Size: 738 1738 3787
Fragments arranged by size:
3787 1738 738
BfrBI ATG'CAT
Cuts at: 1811 1811
Size: 6263
BglI GCCn_nnn'nGGC
Cuts at: 1782 2327 2446 3045 1782
Size: 545 119 599 5000
Fragments arranged by size:
5000 599 545 119
BglII A'GATC_T
Cuts at: 3778 5815 3778
Size: 2037 4226
BlpI GC'TnA_GC
Cuts at: 325 361 1367 3060 3106 3375 325
Size: 36 1006 1693 46 269 3213
Fragments arranged by size:
3213 1693 1006 269 46 36
Bme1580I G_kGCm'C
Cuts at: 1391 1555 2451 2942 2974 3012 3035 3888 3981
Size: 164 896 491 32 38 23 853 93
Cuts at: 3981 5037 1391
Size: 1056 2617
Fragments arranged by size:
2617 1056 896 853 491 164 93 38
32 23
BmrI ACTGGGnnnn_n'
Cuts at: 561 750 2301 2732 2837 3006 3475 3556 3891
Size: 189 1551 431 105 169 469 81 335
Cuts at: 3891 6035 561
Size: 2144 789
Fragments arranged by size:
2144 1551 789 469 431 335 189 169
105 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 294 2349 2490 4630 294
Size: 2055 141 2140 1927
Fragments arranged by size:
2140 2055 1927 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1157 1474 2134 2278 2344 2584 2713 1157
Size: 317 660 144 66 240 129 4707
Fragments arranged by size:
4707 660 317 240 144 129 66
Bpu10I CC'TnA_GC
Cuts at: 2195 2626 2726 2966 2195
Size: 431 100 240 5492
Fragments arranged by size:
5492 431 240 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 1585 3439 4318 4810 5108 949
Size: 636 1854 879 492 298 2104
Fragments arranged by size:
2104 1854 879 636 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1484 5967 1484
Size: 4483 1780
BsaAI yAC'GTr
Cuts at: 414 423 1275 1947 3255 3616 414
Size: 9 852 672 1308 361 3061
Fragments arranged by size:
3061 1308 852 672 361 9
BsaBI GATnn'nnATC
Cuts at: 6 349 1312 3777 5814 6
Size: 343 963 2465 2037 455
Fragments arranged by size:
2465 2037 963 455 343
BsaHI Gr'CG_yC
Cuts at: 1191 2717 2826 3136 3694 3942 4644 6140 1191
Size: 1526 109 310 558 248 702 1496 1314
Fragments arranged by size:
1526 1496 1314 702 558 310 248 109
BsaWI w'CCGG_w
Cuts at: 3182 3452 3973 4651 4925 5072 5483 3182
Size: 270 521 678 274 147 411 3962
Fragments arranged by size:
3962 678 521 411 274 270 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 481 511 633 663 481
Size: 30 122 30 6081
Fragments arranged by size:
6081 122 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 654 700 1849 2008 2505 2773 3063 3102 654
Size: 46 1149 159 497 268 290 39 3815
Fragments arranged by size:
3815 1149 497 290 268 159 46 39
BseYI C'CCAG_C
Cuts at: 1404 1882 2545 2983 3688 4446 5023 5519 1404
Size: 478 663 438 705 758 577 496 2148
Fragments arranged by size:
2148 758 705 663 577 496 478 438
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 720 2800 720
Size: 2080 4183
BsiEI CG_ry'CG
Cuts at: 104 303 1935 2654 3805 3851 5059 104
Size: 199 1632 719 1151 46 1208 1308
Fragments arranged by size:
1632 1308 1208 1151 719 199 46
BsiHKAI G_wGCw'C
Cuts at: 245 419 2075 2201 2632 3125 3277 4055 4245
Size: 174 1656 126 431 493 152 778 190
Cuts at: 4245 5037 245
Size: 792 1471
Fragments arranged by size:
1656 1471 792 778 493 431 190 174
152 126
BsmI GAATG_Cn'
Cuts at: 3166 3166
Size: 6263
Bsp1286I G_dGCh'C
Cuts at: 245 395 419 1391 1555 2000 2075 2102 2173
Size: 150 24 972 164 445 75 27 71
Cuts at: 2173 2201 2451 2542 2632 2942 2950 2974 3012
Size: 28 250 91 90 310 8 24 38
Cuts at: 3012 3023 3035 3125 3277 3888 3981 4055 4245
Size: 11 12 90 152 611 93 74 190
Cuts at: 4245 4307 5037 245
Size: 62 730 1471
Fragments arranged by size:
1471 972 730 611 445 310 250 190
164 152 150 93 91 90 90 75
74 71 62 38 28 27 24 24
12 11 8
BspEI T'CCGG_A
Cuts at: 3182 5483 3182
Size: 2301 3962
BspHI T'CATG_A
Cuts at: 5439 5439
Size: 6263
BspMI ACCTGCnnnn'nnnn_
Cuts at: 662 677 2084 3313 3527 3829 4210 662
Size: 15 1407 1229 214 302 381 2715
Fragments arranged by size:
2715 1407 1229 381 302 214 15
BsrBI CCG'CTC
Cuts at: 50 2140 2418 2513 2730 3105 4555 4609 6093
Size: 2090 278 95 217 375 1450 54 1484
Cuts at: 6093 6258 50
Size: 165 55
Fragments arranged by size:
2090 1484 1450 375 278 217 165 95
55 54
BsrDI GCAATG_nn'
Cuts at: 833 1178 1655 4175 833
Size: 345 477 2520 2921
Fragments arranged by size:
2921 2520 477 345
BsrFI r'CCGG_y
Cuts at: 713 933 2790 3055 4261 4442 4651 713
Size: 220 1857 265 1206 181 209 2325
Fragments arranged by size:
2325 1857 1206 265 220 209 181
BsrGI T'GTAC_A
Cuts at: 2492 2492
Size: 6263
BssSI C'ACGA_G
Cuts at: 268 1098 4534 4892 268
Size: 830 3436 358 1639
Fragments arranged by size:
3436 1639 830 358
BstAPI GCAn_nnn'nTGC
Cuts at: 414 3640 414
Size: 3226 3037
BstBI TT'CG_AA
Cuts at: 110 4624 110
Size: 4514 1749
BstEII G'GTnAC_C
Cuts at: 936 1845 936
Size: 909 5354
BstXI CCAn_nnnn'nTGG
Cuts at: 1483 2163 1483
Size: 680 5583
BstYI r'GATC_y
Cuts at: 1 2996 3179 3288 3755 3778 4113 4359 5371
Size: 2995 183 109 467 23 335 246 1012
Cuts at: 5371 5457 5469 5815 1
Size: 86 12 346 449
Fragments arranged by size:
2995 1012 467 449 346 335 246 183
109 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1832 2517 2849 1832
Size: 685 332 5246
Fragments arranged by size:
5246 685 332
BtgI C'CryG_G
Cuts at: 236 297 303 602 1123 1379 1392 1500 2225
Size: 61 6 299 521 256 13 108 725
Cuts at: 2225 2462 2799 3043 3140 3204 236
Size: 237 337 244 97 64 3295
Fragments arranged by size:
3295 725 521 337 299 256 244 237
108 97 64 61 13 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4213 4394 4557 4213
Size: 181 163 5919
Fragments arranged by size:
5919 181 163
BtsI GCAGTG_nn'
Cuts at: 174 512 1553 1978 2715 3506 174
Size: 338 1041 425 737 791 2931
Fragments arranged by size:
2931 1041 791 737 425 338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 204 239 1146 1181 1989 2024 2232 2267 204
Size: 35 907 35 808 35 208 35 4200
Fragments arranged by size:
4200 907 808 208 35 35 35 35
DraI TTT'AAA
Cuts at: 3262 3262
Size: 6263
DraIII CAC_nnn'GTG
Cuts at: 2559 2559
Size: 6263
DrdI GACnn_nn'nnGTC
Cuts at: 3969 4827 3969
Size: 858 5405
EaeI y'GGCC_r
Cuts at: 300 347 431 1856 1932 2456 2651 2747 3201
Size: 47 84 1425 76 524 195 96 454
Cuts at: 3201 3848 4022 4413 4440 5785 300
Size: 647 174 391 27 1345 778
Fragments arranged by size:
1425 1345 778 647 524 454 391 195
174 96 84 76 47 27
EagI C'GGCC_G
Cuts at: 300 1932 2651 3848 300
Size: 1632 719 1197 2715
Fragments arranged by size:
2715 1632 1197 719
EarI CTCTTCn'nnn_
Cuts at: 250 2590 2759 2890 4286 4496 250
Size: 2340 169 131 1396 210 2017
Fragments arranged by size:
2340 2017 1396 210 169 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1800 2443 4471 4781 4927 6073 1800
Size: 643 2028 310 146 1146 1990
Fragments arranged by size:
2028 1990 1146 643 310 146
EcoICRI GAG'CTC
Cuts at: 2199 2630 3123 3275 2199
Size: 431 493 152 5187
Fragments arranged by size:
5187 493 431 152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1157 1474 2134 2278 2344 2584 2713 4088 4520
Size: 317 660 144 66 240 129 1375 432
Cuts at: 4520 5267 5535 1157
Size: 747 268 1885
Fragments arranged by size:
1885 1375 747 660 432 317 268 240
144 129 66
EcoNI CCTnn'n_nnAGG
Cuts at: 1474 1474
Size: 6263
EcoO109I rG'GnC_Cy
Cuts at: 807 1322 1629 2382 2970 2971 3402 807
Size: 515 307 753 588 1 431 3668
Fragments arranged by size:
3668 753 588 515 431 307 1
EcoRI G'AATT_C
Cuts at: 969 2860 3267 969
Size: 1891 407 3965
Fragments arranged by size:
3965 1891 407
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4282 4282
Size: 6263
FseI GG_CCGG'CC
Cuts at: 2794 2794
Size: 6263
FspI TGC'GCA
Cuts at: 4044 4044
Size: 6263
HaeII r_GCGC'y
Cuts at: 156 1194 1374 2815 2829 3697 3945 4967 5946
Size: 1038 180 1441 14 868 248 1022 979
Cuts at: 5946 156
Size: 473
Fragments arranged by size:
1441 1038 1022 979 868 473 248 180
14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 742 754 774 786 1130 1162 2470 2502 2647
Size: 12 20 12 344 32 1308 32 145
Cuts at: 2647 2679 3058 3090 4105 4135 4137 4167 742
Size: 32 379 32 1015 30 2 30 2838
Fragments arranged by size:
2838 1308 1015 379 344 145 32 32
32 32 30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 3302 3302
Size: 6263
KasI G'GCGC_C
Cuts at: 1190 2825 3693 3941 1190
Size: 1635 868 248 3512
Fragments arranged by size:
3512 1635 868 248
KpnI G_GTAC'C
Cuts at: 3283 3283
Size: 6263
MluI A'CGCG_T
Cuts at: 5696 5696
Size: 6263
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 851 1618 1802 4934 5118 851
Size: 767 184 3132 184 1996
Fragments arranged by size:
3132 1996 767 184 184
MscI TGG'CCA
Cuts at: 349 433 1858 2458 3203 4024 349
Size: 84 1425 600 745 821 2588
Fragments arranged by size:
2588 1425 821 745 600 84
MslI CAynn'nnrTG
Cuts at: 439 1945 2935 4252 4379 439
Size: 1506 990 1317 127 2323
Fragments arranged by size:
2323 1506 1317 990 127
MspA1I CmG'CkG
Cuts at: 305 704 861 867 1125 1432 1502 1725 2801
Size: 399 157 6 258 307 70 223 1076
Cuts at: 2801 3374 3688 4048 5061 5306 305
Size: 573 314 360 1013 245 1262
Fragments arranged by size:
1262 1076 1013 573 399 360 314 307
258 245 223 157 70 6
NaeI GCC'GGC
Cuts at: 715 2792 3057 4444 715
Size: 2077 265 1387 2534
Fragments arranged by size:
2534 2077 1387 265
NarI GG'CG_CC
Cuts at: 1191 2826 3694 3942 1191
Size: 1635 868 248 3512
Fragments arranged by size:
3512 1635 868 248
NcoI C'CATG_G
Cuts at: 2462 2462
Size: 6263
NgoMIV G'CCGG_C
Cuts at: 713 2790 3055 4442 713
Size: 2077 265 1387 2534
Fragments arranged by size:
2534 2077 1387 265
NsiI A_TGCA'T
Cuts at: 1813 1813
Size: 6263
NspI r_CATG'y
Cuts at: 369 1014 3316 3816 4723 369
Size: 645 2302 500 907 1909
Fragments arranged by size:
2302 1909 907 645 500
PasI CC'CwG_GG
Cuts at: 1542 2703 1542
Size: 1161 5102
PciI A'CATG_T
Cuts at: 4719 4719
Size: 6263
PflMI CCAn_nnn'nTGG
Cuts at: 356 704 1200 1542 356
Size: 348 496 342 5077
Fragments arranged by size:
5077 496 348 342
PfoI T'CCnGG_A
Cuts at: 803 1199 1748 803
Size: 396 549 5318
Fragments arranged by size:
5318 549 396
PmeI GTTT'AAAC
Cuts at: 3262 3262
Size: 6263
PmlI CAC'GTG
Cuts at: 1947 1947
Size: 6263
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5402 5434 5402
Size: 32 6231
PpuMI rG'GwC_Cy
Cuts at: 807 1322 2382 807
Size: 515 1060 4688
Fragments arranged by size:
4688 1060 515
PspOMI G'GGCC_C
Cuts at: 2970 2970
Size: 6263
PstI C_TGCA'G
Cuts at: 1763 1968 2154 2274 2438 3310 3995 1763
Size: 205 186 120 164 872 685 4031
Fragments arranged by size:
4031 872 685 205 186 164 120
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6263
PvuII CAG'CTG
Cuts at: 704 1432 1725 3688 4048 704
Size: 728 293 1963 360 2919
Fragments arranged by size:
2919 1963 728 360 293
RsrII CG'GwC_CG
Cuts at: 4458 4458
Size: 6263
SacI G_AGCT'C
Cuts at: 2201 2632 3125 3277 2201
Size: 431 493 152 5187
Fragments arranged by size:
5187 493 431 152
SacII CC_GC'GG
Cuts at: 306 1126 1503 2802 306
Size: 820 377 1299 3767
Fragments arranged by size:
3767 1299 820 377
SalI G'TCGA_C
Cuts at: 3300 3300
Size: 6263
SanDI GG'GwC_CC
Cuts at: 1322 1322
Size: 6263
SapI GCTCTTCn'nnn_
Cuts at: 250 4286 4496 250
Size: 4036 210 2017
Fragments arranged by size:
4036 2017 210
SbfI CC_TGCA'GG
Cuts at: 3310 3310
Size: 6263
ScaI AGT'ACT
Cuts at: 490 5780 490
Size: 5290 973
SexAI A'CCwGG_T
Cuts at: 217 287 3026 217
Size: 70 2739 3454
Fragments arranged by size:
3454 2739 70
SfcI C'TryA_G
Cuts at: 33 1759 1895 1964 2150 2270 2434 3306 3991
Size: 1726 136 69 186 120 164 872 685
Cuts at: 3991 4984 5175 33
Size: 993 191 1121
Fragments arranged by size:
1726 1121 993 872 685 191 186 164
136 120 69
SfoI GGC'GCC
Cuts at: 1192 2827 3695 3943 1192
Size: 1635 868 248 3512
Fragments arranged by size:
3512 1635 868 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6263
SmaI CCC'GGG
Cuts at: 132 3285 132
Size: 3153 3110
SmlI C'TyrA_G
Cuts at: 964 1600 3418 4333 4825 5087 964
Size: 636 1818 915 492 262 2140
Fragments arranged by size:
2140 1818 915 636 492 262
SnaBI TAC'GTA
Cuts at: 3255 3255
Size: 6263
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6263
SphI G_CATG'C
Cuts at: 369 3316 3816 369
Size: 2947 500 2816
Fragments arranged by size:
2947 2816 500
StuI AGG'CCT
Cuts at: 274 1480 2032 274
Size: 1206 552 4505
Fragments arranged by size:
4505 1206 552
StyI C'CwwG_G
Cuts at: 1334 1661 2156 2462 3397 3751 1334
Size: 327 495 306 935 354 3846
Fragments arranged by size:
3846 935 495 354 327 306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1079 3846 3979 4438 4647 1079
Size: 2767 133 459 209 2695
Fragments arranged by size:
2767 2695 459 209 133
TatI w'GTAC_w
Cuts at: 488 1436 2492 5778 488
Size: 948 1056 3286 973
Fragments arranged by size:
3286 1056 973 948
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5780 5812 5780
Size: 32 6231
Tth111I GACn'n_nGTC
Cuts at: 178 1228 1326 1699 4060 178
Size: 1050 98 373 2361 2381
Fragments arranged by size:
2381 2361 1050 373 98
XbaI T'CTAG_A
Cuts at: 3294 3294
Size: 6263
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3758 3758
Size: 6263
XmaI C'CCGG_G
Cuts at: 130 3283 130
Size: 3153 3110
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AscI AvrII BaeI
BmgBI BmtI BsiWI BsmBI BssHII BstZ17I ClaI EcoRV
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PshAI PsiI PspXI PsrI SfiI SgrAI SrfI
SspI SwaI XhoI XmnI ZraI