Restriction map for
pF1KSDA0661
(Circular) (Six-base) MAPSORT of: pF1KSDA0661.seq Check: 3840 from: 1 to: 6140
pF1KSDA0661 6140 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1403 2351 1403
Size: 948 5192
AccI GT'mk_AC
Cuts at: 2906 3178 2906
Size: 272 5868
Acc65I G'GTAC_C
Cuts at: 3156 3156
Size: 6140
AclI AA'CG_TT
Cuts at: 5980 5980
Size: 6140
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 467 1189 1835 1901 2764 3965 4397 5144
Size: 175 722 646 66 863 1201 432 747
Cuts at: 5144 5412 292
Size: 268 1020
Fragments arranged by size:
1201 1020 863 747 722 646 432 268
175 66
AflIII A'CryG_T
Cuts at: 520 3492 4596 5573 520
Size: 2972 1104 977 1087
Fragments arranged by size:
2972 1104 1087 977
AgeI A'CCGG_T
Cuts at: 4528 4528
Size: 6140
AleI CACnn'nnGTG
Cuts at: 1852 1852
Size: 6140
AlwNI CAG_nnn'CTG
Cuts at: 490 1158 1533 1786 1828 2601 2616 3445 5012
Size: 668 375 253 42 773 15 829 1567
Cuts at: 5012 490
Size: 1618
Fragments arranged by size:
1618 1567 829 773 668 375 253 42
15
ApaI G_GGCC'C
Cuts at: 176 423 1559 1686 1914 1921 176
Size: 247 1136 127 228 7 4395
Fragments arranged by size:
4395 1136 247 228 127 7
ApaLI G'TGCA_C
Cuts at: 4910 4910
Size: 6140
ApoI r'AATT_y
Cuts at: 3144 5493 5749 3144
Size: 2349 256 3535
Fragments arranged by size:
3535 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6140
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6140
AvaI C'yCGr_G
Cuts at: 815 1361 1928 2650 3160 815
Size: 546 567 722 510 3795
Fragments arranged by size:
3795 722 567 546 510
AvrII C'CTAG_G
Cuts at: 1989 1989
Size: 6140
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 268 301 268
Size: 33 6107
BamHI G'GATC_C
Cuts at: 3165 3165
Size: 6140
BanI G'GyrC_C
Cuts at: 495 634 1831 2359 3097 3156 3570 3818 3853
Size: 139 1197 528 738 59 414 248 35
Cuts at: 3853 495
Size: 2782
Fragments arranged by size:
2782 1197 738 528 414 248 139 59
35
BanII G_rGCy'C
Cuts at: 169 176 230 423 788 908 1075 1559 1686
Size: 7 54 193 365 120 167 484 127
Cuts at: 1686 1914 1921 2102 2699 2850 3154 4184 169
Size: 228 7 181 597 151 304 1030 2125
Fragments arranged by size:
2125 1030 597 484 365 304 228 193
181 167 151 127 120 54 7 7
BbeI G_GCGC'C
Cuts at: 3574 3822 3574
Size: 248 5892
BbsI GAAGACnn'nnnn_
Cuts at: 211 473 1348 1585 211
Size: 262 875 237 4766
Fragments arranged by size:
4766 875 262 237
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2268 2302 2268
Size: 34 6106
BciVI GTATCCnnnnn_n'
Cuts at: 4038 4799 6140 4038
Size: 761 1341 4038
Fragments arranged by size:
4038 1341 761
BclI T'GATC_A
Cuts at: 3660 3660
Size: 6140
BglI GCCn_nnn'nGGC
Cuts at: 2731 2731
Size: 6140
BglII A'GATC_T
Cuts at: 3655 5692 3655
Size: 2037 4103
BlpI GC'TnA_GC
Cuts at: 3252 3252
Size: 6140
Bme1580I G_kGCm'C
Cuts at: 176 423 1559 1686 1752 1914 1921 3076 3102
Size: 247 1136 127 66 162 7 1155 26
Cuts at: 3102 3765 3858 4914 176
Size: 663 93 1056 1402
Fragments arranged by size:
1402 1155 1136 1056 663 247 162 127
93 66 26 7
BmrI ACTGGGnnnn_n'
Cuts at: 426 640 3352 3433 3768 5912 426
Size: 214 2712 81 335 2144 654
Fragments arranged by size:
2712 2144 654 335 214 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2116 2877 4507 2116
Size: 761 1630 3749
Fragments arranged by size:
3749 1630 761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 892 964 1204 1489 1805 2638 2713 2776
Size: 210 72 240 285 316 833 75 63
Cuts at: 2776 2914 682
Size: 138 3908
Fragments arranged by size:
3908 833 316 285 240 210 138 75
72 63
Bpu10I CC'TnA_GC
Cuts at: 168 803 2213 2849 168
Size: 635 1410 636 3459
Fragments arranged by size:
3459 1410 636 635
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1219 2004 2065 2086 2560 2824 3316 4195 4687
Size: 785 61 21 474 264 492 879 492
Cuts at: 4687 4985 1219
Size: 298 2374
Fragments arranged by size:
2374 879 785 492 492 474 298 264
61 21
BsaI GGTCTCn'nnnn_
Cuts at: 560 925 2092 5844 560
Size: 365 1167 3752 856
Fragments arranged by size:
3752 1167 856 365
BsaAI yAC'GTr
Cuts at: 2763 3132 3493 2763
Size: 369 361 5410
Fragments arranged by size:
5410 369 361
BsaBI GATnn'nnATC
Cuts at: 6 3654 5691 6
Size: 3648 2037 455
Fragments arranged by size:
3648 2037 455
BsaHI Gr'CG_yC
Cuts at: 1625 1742 3571 3819 4521 6017 1625
Size: 117 1829 248 702 1496 1748
Fragments arranged by size:
1829 1748 1496 702 248 117
BsaWI w'CCGG_w
Cuts at: 3329 3850 4528 4802 4949 5360 3329
Size: 521 678 274 147 411 4109
Fragments arranged by size:
4109 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 274 388 524 664 1065 1276 1783 1879
Size: 60 114 136 140 401 211 507 96
Cuts at: 1879 2068 2272 2341 2389 2392 2554 2635 2950
Size: 189 204 69 48 3 162 81 315
Cuts at: 2950 214
Size: 3404
Fragments arranged by size:
3404 507 401 315 211 204 189 162
140 136 114 96 81 69 60 48
3
BseYI C'CCAG_C
Cuts at: 422 822 1346 1428 1628 1752 1984 2355 2594
Size: 400 524 82 200 124 232 371 239
Cuts at: 2594 2726 3565 4323 4900 5396 422
Size: 132 839 758 577 496 1166
Fragments arranged by size:
1166 839 758 577 524 496 400 371
239 232 200 132 124 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2206 2629 2770 2785 2206
Size: 423 141 15 5561
Fragments arranged by size:
5561 423 141 15
BsiEI CG_ry'CG
Cuts at: 104 3682 3728 4936 104
Size: 3578 46 1208 1308
Fragments arranged by size:
3578 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 908 1075 1412 2102 2699 2762 3154 3932 4122
Size: 167 337 690 597 63 392 778 190
Cuts at: 4122 4914 908
Size: 792 2134
Fragments arranged by size:
2134 792 778 690 597 392 337 190
167 63
BsmI GAATG_Cn'
Cuts at: 1137 2312 1137
Size: 1175 4965
BsmBI CGTCTCn'nnnn_
Cuts at: 1270 1270
Size: 6140
Bsp1286I G_dGCh'C
Cuts at: 169 176 230 423 788 908 1075 1412 1559
Size: 7 54 193 365 120 167 337 147
Cuts at: 1559 1686 1752 1914 1921 2102 2699 2762 2850
Size: 127 66 162 7 181 597 63 88
Cuts at: 2850 3076 3102 3154 3765 3858 3932 4122 4184
Size: 226 26 52 611 93 74 190 62
Cuts at: 4184 4914 169
Size: 730 1395
Fragments arranged by size:
1395 730 611 597 365 337 226 193
190 181 167 162 147 127 120 93
88 74 66 63 62 54 52 26
7 7
BspEI T'CCGG_A
Cuts at: 5360 5360
Size: 6140
BspHI T'CATG_A
Cuts at: 5316 5316
Size: 6140
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1403 2351 2553 2974 3190 3404 3706 4087 1403
Size: 948 202 421 216 214 302 381 3456
Fragments arranged by size:
3456 948 421 381 302 216 214 202
BsrBI CCG'CTC
Cuts at: 50 309 2014 2502 4432 4486 5970 6135 50
Size: 259 1705 488 1930 54 1484 165 55
Fragments arranged by size:
1930 1705 1484 488 259 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4052 4052
Size: 6140
BsrFI r'CCGG_y
Cuts at: 154 606 836 4138 4319 4528 154
Size: 452 230 3302 181 209 1766
Fragments arranged by size:
3302 1766 452 230 209 181
BsrGI T'GTAC_A
Cuts at: 764 2150 764
Size: 1386 4754
BssSI C'ACGA_G
Cuts at: 704 2816 4411 4769 704
Size: 2112 1595 358 2075
Fragments arranged by size:
2112 2075 1595 358
BstAPI GCAn_nnn'nTGC
Cuts at: 634 3517 634
Size: 2883 3257
BstBI TT'CG_AA
Cuts at: 110 4501 110
Size: 4391 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 942 1693 942
Size: 751 5389
BstYI r'GATC_y
Cuts at: 1 2783 3165 3632 3655 3990 4236 5248 5334
Size: 2782 382 467 23 335 246 1012 86
Cuts at: 5334 5346 5692 1
Size: 12 346 449
Fragments arranged by size:
2782 1012 467 449 382 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 491 1077 1259 1943 491
Size: 586 182 684 4688
Fragments arranged by size:
4688 684 586 182
BtgI C'CryG_G
Cuts at: 254 254
Size: 6140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4090 4271 4434 4090
Size: 181 163 5796
Fragments arranged by size:
5796 181 163
BtsI GCAGTG_nn'
Cuts at: 651 979 1312 3383 651
Size: 328 333 2071 3408
Fragments arranged by size:
3408 2071 333 328
DraI TTT'AAA
Cuts at: 3139 3139
Size: 6140
DraIII CAC_nnn'GTG
Cuts at: 634 634
Size: 6140
DrdI GACnn_nn'nnGTC
Cuts at: 3846 4704 3846
Size: 858 5282
EaeI y'GGCC_r
Cuts at: 854 1160 1376 1538 2732 3725 3899 4290 4317
Size: 306 216 162 1194 993 174 391 27
Cuts at: 4317 5662 854
Size: 1345 1332
Fragments arranged by size:
1345 1332 1194 993 391 306 216 174
162 27
EagI C'GGCC_G
Cuts at: 3725 3725
Size: 6140
EarI CTCTTCn'nnn_
Cuts at: 255 1877 4163 4373 255
Size: 1622 2286 210 2022
Fragments arranged by size:
2286 2022 1622 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4348 4658 4804 5950 4348
Size: 310 146 1146 4538
Fragments arranged by size:
4538 1146 310 146
EcoICRI GAG'CTC
Cuts at: 906 1073 2100 2697 3152 906
Size: 167 1027 597 455 3894
Fragments arranged by size:
3894 1027 597 455 167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 467 682 892 964 1189 1204 1489 1805
Size: 175 215 210 72 225 15 285 316
Cuts at: 1805 1835 1901 2638 2713 2764 2776 2914 3965
Size: 30 66 737 75 51 12 138 1051
Cuts at: 3965 4397 5144 5412 292
Size: 432 747 268 1020
Fragments arranged by size:
1051 1020 747 737 432 316 285 268
225 215 210 175 138 75 72 66
51 30 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 793 1027 1258 2932 793
Size: 234 231 1674 4001
Fragments arranged by size:
4001 1674 234 231
EcoO109I rG'GnC_Cy
Cuts at: 172 173 503 1365 1555 1783 1910 1917 1918
Size: 1 330 862 190 228 127 7 1
Cuts at: 1918 1981 1993 2565 2739 3279 172
Size: 63 12 572 174 540 3033
Fragments arranged by size:
3033 862 572 540 330 228 190 174
127 63 12 7 1 1
EcoRI G'AATT_C
Cuts at: 3144 3144
Size: 6140
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4159 4159
Size: 6140
FspI TGC'GCA
Cuts at: 1513 2680 3921 1513
Size: 1167 1241 3732
Fragments arranged by size:
3732 1241 1167
HaeII r_GCGC'y
Cuts at: 490 738 1233 2164 3574 3822 4844 5823 490
Size: 248 495 931 1410 248 1022 979 807
Fragments arranged by size:
1410 1022 979 931 807 495 248 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 183 1575 1607 3982 4012 4014 4044 151
Size: 32 1392 32 2375 30 2 30 2247
Fragments arranged by size:
2375 2247 1392 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2963 3179 2963
Size: 216 5924
HindIII A'AGCT_T
Cuts at: 1645 1645
Size: 6140
KasI G'GCGC_C
Cuts at: 3570 3818 3570
Size: 248 5892
KpnI G_GTAC'C
Cuts at: 3160 3160
Size: 6140
MluI A'CGCG_T
Cuts at: 5573 5573
Size: 6140
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 344 4811 4995 344
Size: 4467 184 1489
Fragments arranged by size:
4467 1489 184
MscI TGG'CCA
Cuts at: 856 1162 1378 3901 856
Size: 306 216 2523 3095
Fragments arranged by size:
3095 2523 306 216
MslI CAynn'nnrTG
Cuts at: 1852 4129 4256 1852
Size: 2277 127 3736
Fragments arranged by size:
3736 2277 127
MspA1I CmG'CkG
Cuts at: 426 585 697 864 1467 2175 2200 2598 2730
Size: 159 112 167 603 708 25 398 132
Cuts at: 2730 2754 3251 3565 3925 4938 5183 426
Size: 24 497 314 360 1013 245 1383
Fragments arranged by size:
1383 1013 708 603 497 398 360 314
245 167 159 132 112 25 24
NaeI GCC'GGC
Cuts at: 156 608 4321 156
Size: 452 3713 1975
Fragments arranged by size:
3713 1975 452
NarI GG'CG_CC
Cuts at: 3571 3819 3571
Size: 248 5892
NgoMIV G'CCGG_C
Cuts at: 154 606 4319 154
Size: 452 3713 1975
Fragments arranged by size:
3713 1975 452
NspI r_CATG'y
Cuts at: 524 1113 1296 2430 3193 3693 4600 524
Size: 589 183 1134 763 500 907 2064
Fragments arranged by size:
2064 1134 907 763 589 500 183
PasI CC'CwG_GG
Cuts at: 1759 1906 1914 1759
Size: 147 8 5985
Fragments arranged by size:
5985 147 8
PciI A'CATG_T
Cuts at: 520 4596 520
Size: 4076 2064
PflMI CCAn_nnn'nTGG
Cuts at: 1158 1828 2691 1158
Size: 670 863 4607
Fragments arranged by size:
4607 863 670
PfoI T'CCnGG_A
Cuts at: 908 2246 2930 908
Size: 1338 684 4118
Fragments arranged by size:
4118 1338 684
PmeI GTTT'AAAC
Cuts at: 3139 3139
Size: 6140
PmlI CAC'GTG
Cuts at: 2763 2763
Size: 6140
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5279 5311 5279
Size: 32 6108
PpuMI rG'GwC_Cy
Cuts at: 503 1981 1993 2565 2739 503
Size: 1478 12 572 174 3904
Fragments arranged by size:
3904 1478 572 174 12
PspOMI G'GGCC_C
Cuts at: 172 419 1555 1682 1910 1917 172
Size: 247 1136 127 228 7 4395
Fragments arranged by size:
4395 1136 247 228 127 7
PstI C_TGCA'G
Cuts at: 848 869 1196 1301 1439 2348 3187 3872 848
Size: 21 327 105 138 909 839 685 3116
Fragments arranged by size:
3116 909 839 685 327 138 105 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6140
PvuII CAG'CTG
Cuts at: 426 585 697 864 1467 2598 2730 2754 3565
Size: 159 112 167 603 1131 132 24 811
Cuts at: 3565 3925 426
Size: 360 2641
Fragments arranged by size:
2641 1131 811 603 360 167 159 132
112 24
RsrII CG'GwC_CG
Cuts at: 4335 4335
Size: 6140
SacI G_AGCT'C
Cuts at: 908 1075 2102 2699 3154 908
Size: 167 1027 597 455 3894
Fragments arranged by size:
3894 1027 597 455 167
SalI G'TCGA_C
Cuts at: 3177 3177
Size: 6140
SanDI GG'GwC_CC
Cuts at: 503 503
Size: 6140
SapI GCTCTTCn'nnn_
Cuts at: 4163 4373 4163
Size: 210 5930
SbfI CC_TGCA'GG
Cuts at: 3187 3187
Size: 6140
ScaI AGT'ACT
Cuts at: 898 5657 898
Size: 4759 1381
SfcI C'TryA_G
Cuts at: 33 280 730 844 865 1192 1297 1435 2003
Size: 247 450 114 21 327 105 138 568
Cuts at: 2003 2344 2452 2714 3183 3868 4861 5052 33
Size: 341 108 262 469 685 993 191 1121
Fragments arranged by size:
1121 993 685 568 469 450 341 327
262 247 191 138 114 108 105 21
SfoI GGC'GCC
Cuts at: 3572 3820 3572
Size: 248 5892
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6140
SgrAI Cr'CCGG_yG
Cuts at: 154 154
Size: 6140
SmaI CCC'GGG
Cuts at: 1930 3162 1930
Size: 1232 4908
SmlI C'TyrA_G
Cuts at: 1234 2019 2080 2101 2575 2839 3295 4210 4702
Size: 785 61 21 474 264 456 915 492
Cuts at: 4702 4964 1234
Size: 262 2410
Fragments arranged by size:
2410 915 785 492 474 456 264 262
61 21
SnaBI TAC'GTA
Cuts at: 3132 3132
Size: 6140
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6140
SphI G_CATG'C
Cuts at: 1113 1296 3193 3693 1113
Size: 183 1897 500 3560
Fragments arranged by size:
3560 1897 500 183
StuI AGG'CCT
Cuts at: 718 1033 718
Size: 315 5825
StyI C'CwwG_G
Cuts at: 611 689 935 1989 2042 2165 2568 3274 3628
Size: 78 246 1054 53 123 403 706 354
Cuts at: 3628 611
Size: 3123
Fragments arranged by size:
3123 1054 706 403 354 246 123 78
53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 622 1742 2347 3723 3856 4315 4524 622
Size: 1120 605 1376 133 459 209 2238
Fragments arranged by size:
2238 1376 1120 605 459 209 133
TatI w'GTAC_w
Cuts at: 764 896 1656 2150 2948 5655 764
Size: 132 760 494 798 2707 1249
Fragments arranged by size:
2707 1249 798 760 494 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 958 990 5657 5689 958
Size: 32 4667 32 1409
Fragments arranged by size:
4667 1409 32 32
Tth111I GACn'n_nGTC
Cuts at: 3937 3937
Size: 6140
XbaI T'CTAG_A
Cuts at: 3171 3171
Size: 6140
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 261 1170 1716 3635 261
Size: 909 546 1919 2766
Fragments arranged by size:
2766 1919 909 546
XmaI C'CCGG_G
Cuts at: 1928 3160 1928
Size: 1232 4908
XmnI GAAnn'nnTTC
Cuts at: 446 446
Size: 6140
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI BbvCI
BfrBI BmgBI BmtI BsaXI BsiWI BssHII BstEII BstZ17I
ClaI CspCI EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SrfI SspI SwaI
XhoI ZraI