Restriction map for
pF1KSDA0663
(Circular) (Six-base) MAPSORT of: pF1KSDA0663.seq Check: 354 from: 1 to: 5569
pF1KSDA0663 5569 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 2605 2605
Size: 5569
Acc65I G'GTAC_C
Cuts at: 2583 2583
Size: 5569
AclI AA'CG_TT
Cuts at: 5409 5409
Size: 5569
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 818 1286 1678 1736 2297 2312 3392 3824
Size: 114 468 392 58 561 15 1080 432
Cuts at: 3824 4573 4841 704
Size: 749 268 1432
Fragments arranged by size:
1432 1080 749 561 468 432 392 268
114 58 15
AflII C'TTAA_G
Cuts at: 1117 1117
Size: 5569
AflIII A'CryG_T
Cuts at: 169 2919 4025 5002 169
Size: 2750 1106 977 736
Fragments arranged by size:
2750 1106 977 736
AgeI A'CCGG_T
Cuts at: 3957 3957
Size: 5569
AleI CACnn'nnGTG
Cuts at: 1924 1924
Size: 5569
AlwNI CAG_nnn'CTG
Cuts at: 880 2269 2872 4441 880
Size: 1389 603 1569 2008
Fragments arranged by size:
2008 1569 1389 603
ApaLI G'TGCA_C
Cuts at: 1531 4339 1531
Size: 2808 2761
ApoI r'AATT_y
Cuts at: 314 756 988 1214 1784 2571 4922 5178 314
Size: 442 232 226 570 787 2351 256 705
Fragments arranged by size:
2351 787 705 570 442 256 232 226
AseI AT'TA_AT
Cuts at: 20 596 20
Size: 576 4993
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5569
AvaI C'yCGr_G
Cuts at: 2587 2587
Size: 5569
BamHI G'GATC_C
Cuts at: 2499 2592 2499
Size: 93 5476
BanI G'GyrC_C
Cuts at: 1943 2583 2997 3245 3280 1943
Size: 640 414 248 35 4232
Fragments arranged by size:
4232 640 414 248 35
BanII G_rGCy'C
Cuts at: 869 1591 2581 3611 869
Size: 722 990 1030 2827
Fragments arranged by size:
2827 1030 990 722
BbeI G_GCGC'C
Cuts at: 3001 3249 3001
Size: 248 5321
BbsI GAAGACnn'nnnn_
Cuts at: 149 1207 149
Size: 1058 4511
BbvCI CC'TCA_GC
Cuts at: 524 524
Size: 5569
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 254 288 254
Size: 34 5535
BciVI GTATCCnnnnn_n'
Cuts at: 1401 3465 4228 5569 1401
Size: 2064 763 1341 1401
Fragments arranged by size:
2064 1401 1341 763
BclI T'GATC_A
Cuts at: 627 3087 627
Size: 2460 3109
BglI GCCn_nnn'nGGC
Cuts at: 2221 2221
Size: 5569
BglII A'GATC_T
Cuts at: 3082 5121 3082
Size: 2039 3530
BlpI GC'TnA_GC
Cuts at: 2679 2679
Size: 5569
Bme1580I G_kGCm'C
Cuts at: 1535 3192 3285 4343 1535
Size: 1657 93 1058 2761
Fragments arranged by size:
2761 1657 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 985 2313 2779 2860 3195 5341 985
Size: 1328 466 81 335 2146 1213
Fragments arranged by size:
2146 1328 1213 466 335 81
BmtI G_CTAG'C
Cuts at: 473 473
Size: 5569
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3934 3934
Size: 5569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1049 1582 1616 2172 1049
Size: 533 34 556 4446
Fragments arranged by size:
4446 556 533 34
Bpu10I CC'TnA_GC
Cuts at: 524 863 524
Size: 339 5230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 1085 1249 2551 2743 3622 4116 4414 796
Size: 289 164 1302 192 879 494 298 1951
Fragments arranged by size:
1951 1302 879 494 298 289 192 164
BsaI GGTCTCn'nnnn_
Cuts at: 1753 1798 5273 1753
Size: 45 3475 2049
Fragments arranged by size:
3475 2049 45
BsaAI yAC'GTr
Cuts at: 2559 2920 2559
Size: 361 5208
BsaBI GATnn'nnATC
Cuts at: 6 626 3081 5120 6
Size: 620 2455 2039 455
Fragments arranged by size:
2455 2039 620 455
BsaHI Gr'CG_yC
Cuts at: 2998 3246 3950 5446 2998
Size: 248 704 1496 3121
Fragments arranged by size:
3121 1496 704 248
BsaWI w'CCGG_w
Cuts at: 716 2756 3277 3957 4231 4378 4789 716
Size: 2040 521 680 274 147 411 1496
Fragments arranged by size:
2040 1496 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1308 1510 1308
Size: 202 5367
BseYI C'CCAG_C
Cuts at: 2211 2992 3750 4329 4825 2211
Size: 781 758 579 496 2955
Fragments arranged by size:
2955 781 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 1600 2054 294
Size: 1306 454 3809
Fragments arranged by size:
3809 1306 454
BsiEI CG_ry'CG
Cuts at: 104 3109 3155 4365 104
Size: 3005 46 1210 1308
Fragments arranged by size:
3005 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1535 1591 1919 2581 3359 3549 4343 1535
Size: 56 328 662 778 190 794 2761
Fragments arranged by size:
2761 794 778 662 328 190 56
BsmBI CGTCTCn'nnnn_
Cuts at: 1619 1619
Size: 5569
Bsp1286I G_dGCh'C
Cuts at: 869 1535 1591 1919 2581 3192 3285 3359 3549
Size: 666 56 328 662 611 93 74 190
Cuts at: 3549 3611 4343 869
Size: 62 732 2095
Fragments arranged by size:
2095 732 666 662 611 328 190 93
74 62 56
BspEI T'CCGG_A
Cuts at: 716 4789 716
Size: 4073 1496
BspHI T'CATG_A
Cuts at: 4745 4745
Size: 5569
BspMI ACCTGCnnnn'nnnn_
Cuts at: 266 732 1495 2617 2831 3133 3514 266
Size: 466 763 1122 214 302 381 2321
Fragments arranged by size:
2321 1122 763 466 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3859 3913 5399 5564 50
Size: 3809 54 1486 165 55
Fragments arranged by size:
3809 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2162 3479 2162
Size: 1317 4252
BsrFI r'CCGG_y
Cuts at: 3565 3746 3957 3565
Size: 181 211 5177
Fragments arranged by size:
5177 211 181
BssSI C'ACGA_G
Cuts at: 1621 3838 4198 1621
Size: 2217 360 2992
Fragments arranged by size:
2992 2217 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1530 2944 1530
Size: 1414 4155
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5569
BstYI r'GATC_y
Cuts at: 1 1190 2499 2592 3059 3082 3417 3663 4677
Size: 1189 1309 93 467 23 335 246 1014
Cuts at: 4677 4763 4775 5121 1
Size: 86 12 346 449
Fragments arranged by size:
1309 1189 1014 467 449 346 335 246
93 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1998 1998
Size: 5569
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3517 3698 3861 3517
Size: 181 163 5225
Fragments arranged by size:
5225 181 163
BtsI GCAGTG_nn'
Cuts at: 315 1919 2147 2199 2810 315
Size: 1604 228 52 611 3074
Fragments arranged by size:
3074 1604 611 228 52
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1752 1787 1752
Size: 35 5534
DraI TTT'AAA
Cuts at: 2566 2566
Size: 5569
DrdI GACnn_nn'nnGTC
Cuts at: 2263 3273 4133 2263
Size: 1010 860 3699
Fragments arranged by size:
3699 1010 860
EaeI y'GGCC_r
Cuts at: 3152 3326 3717 3744 5091 3152
Size: 174 391 27 1347 3630
Fragments arranged by size:
3630 1347 391 174 27
EagI C'GGCC_G
Cuts at: 3152 3152
Size: 5569
EarI CTCTTCn'nnn_
Cuts at: 449 1676 1871 2331 3590 3800 449
Size: 1227 195 460 1259 210 2218
Fragments arranged by size:
2218 1259 1227 460 210 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3775 4087 4233 5379 3775
Size: 312 146 1146 3965
Fragments arranged by size:
3965 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1589 2579 1589
Size: 990 4579
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 818 1049 1286 1582 1616 1678 1736 2172
Size: 114 231 237 296 34 62 58 436
Cuts at: 2172 2297 2312 3392 3824 4573 4841 704
Size: 125 15 1080 432 749 268 1432
Fragments arranged by size:
1432 1080 749 436 432 296 268 237
231 125 114 62 58 34 15
EcoNI CCTnn'n_nnAGG
Cuts at: 134 134
Size: 5569
EcoO109I rG'GnC_Cy
Cuts at: 877 1276 1350 2706 877
Size: 399 74 1356 3740
Fragments arranged by size:
3740 1356 399 74
EcoRI G'AATT_C
Cuts at: 2571 2571
Size: 5569
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1234 2539 3586 1234
Size: 1305 1047 3217
Fragments arranged by size:
3217 1305 1047
FspI TGC'GCA
Cuts at: 3348 3348
Size: 5569
HaeII r_GCGC'y
Cuts at: 258 3001 3249 4273 5252 258
Size: 2743 248 1024 979 575
Fragments arranged by size:
2743 1024 979 575 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 903 935 1760 1792 1867 1899 2245 2277 3409
Size: 32 825 32 75 32 346 32 1132
Cuts at: 3409 3439 3441 3471 903
Size: 30 2 30 3001
Fragments arranged by size:
3001 1132 825 346 75 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1773 2606 1773
Size: 833 4736
KasI G'GCGC_C
Cuts at: 2997 3245 2997
Size: 248 5321
KpnI G_GTAC'C
Cuts at: 2587 2587
Size: 5569
MluI A'CGCG_T
Cuts at: 5002 5002
Size: 5569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2338 4240 4424 2338
Size: 1902 184 3483
Fragments arranged by size:
3483 1902 184
MscI TGG'CCA
Cuts at: 3328 3328
Size: 5569
MslI CAynn'nnrTG
Cuts at: 1924 3556 3683 1924
Size: 1632 127 3810
Fragments arranged by size:
3810 1632 127
MspA1I CmG'CkG
Cuts at: 188 528 1141 2170 2227 2678 2992 3352 4367
Size: 340 613 1029 57 451 314 360 1015
Cuts at: 4367 4612 188
Size: 245 1145
Fragments arranged by size:
1145 1029 1015 613 451 360 340 314
245 57
NaeI GCC'GGC
Cuts at: 3748 3748
Size: 5569
NarI GG'CG_CC
Cuts at: 2998 3246 2998
Size: 248 5321
NgoMIV G'CCGG_C
Cuts at: 3746 3746
Size: 5569
NheI G'CTAG_C
Cuts at: 469 469
Size: 5569
NruI TCG'CGA
Cuts at: 3930 3930
Size: 5569
NspI r_CATG'y
Cuts at: 173 1137 1827 2620 3120 4029 173
Size: 964 690 793 500 909 1713
Fragments arranged by size:
1713 964 909 793 690 500
PciI A'CATG_T
Cuts at: 169 4025 169
Size: 3856 1713
PflMI CCAn_nnn'nTGG
Cuts at: 1983 1983
Size: 5569
PmeI GTTT'AAAC
Cuts at: 2566 2566
Size: 5569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4708 4740 4708
Size: 32 5537
PpuMI rG'GwC_Cy
Cuts at: 877 1276 1350 877
Size: 399 74 5096
Fragments arranged by size:
5096 399 74
PshAI GACnn'nnGTC
Cuts at: 712 712
Size: 5569
PstI C_TGCA'G
Cuts at: 606 729 1506 2614 3299 606
Size: 123 777 1108 685 2876
Fragments arranged by size:
2876 1108 777 685 123
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5569
PvuII CAG'CTG
Cuts at: 188 528 1141 2227 2992 3352 188
Size: 340 613 1086 765 360 2405
Fragments arranged by size:
2405 1086 765 613 360 340
RsrII CG'GwC_CG
Cuts at: 3762 3762
Size: 5569
SacI G_AGCT'C
Cuts at: 1591 2581 1591
Size: 990 4579
SalI G'TCGA_C
Cuts at: 2604 2604
Size: 5569
SapI GCTCTTCn'nnn_
Cuts at: 3590 3800 3590
Size: 210 5359
SbfI CC_TGCA'GG
Cuts at: 1506 2614 1506
Size: 1108 4461
ScaI AGT'ACT
Cuts at: 5086 5086
Size: 5569
SfcI C'TryA_G
Cuts at: 33 602 725 1502 2200 2610 3295 4290 4481
Size: 569 123 777 698 410 685 995 191
Cuts at: 4481 33
Size: 1121
Fragments arranged by size:
1121 995 777 698 685 569 410 191
123
SfoI GGC'GCC
Cuts at: 2999 3247 2999
Size: 248 5321
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5569
SmaI CCC'GGG
Cuts at: 2589 2589
Size: 5569
SmlI C'TyrA_G
Cuts at: 775 1100 1117 1264 2530 2722 3637 4131 4393
Size: 325 17 147 1266 192 915 494 262
Cuts at: 4393 775
Size: 1951
Fragments arranged by size:
1951 1266 915 494 325 262 192 147
17
SnaBI TAC'GTA
Cuts at: 2559 2559
Size: 5569
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5569
SphI G_CATG'C
Cuts at: 2620 3120 2620
Size: 500 5069
StyI C'CwwG_G
Cuts at: 947 2045 2701 3055 947
Size: 1098 656 354 3461
Fragments arranged by size:
3461 1098 656 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1482 3150 3283 3742 3953 1482
Size: 1668 133 459 211 3098
Fragments arranged by size:
3098 1668 459 211 133
TatI w'GTAC_w
Cuts at: 5084 5084
Size: 5569
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 689 721 5086 5118 689
Size: 32 4365 32 1140
Fragments arranged by size:
4365 1140 32 32
Tth111I GACn'n_nGTC
Cuts at: 3364 3364
Size: 5569
XbaI T'CTAG_A
Cuts at: 2598 2598
Size: 5569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3062 3062
Size: 5569
XmaI C'CCGG_G
Cuts at: 2587 2587
Size: 5569
XmnI GAAnn'nnTTC
Cuts at: 563 990 1214 1217 563
Size: 427 224 3 4915
Fragments arranged by size:
4915 427 224 3
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NruI NspI
PciI PflMI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BfrBI BmgBI BsaXI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I BtgI ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NotI
NsiI PacI PasI PfoI PmlI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI
StuI SwaI XhoI ZraI