Restriction map for
pF1KSDA0665
(Circular) (Six-base) MAPSORT of: pF1KSDA0665.seq Check: 2213 from: 1 to: 5407
pF1KSDA0665 5407 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 828 2027 828
Size: 1199 4208
AatII G_ACGT'C
Cuts at: 605 605
Size: 5407
AccI GT'mk_AC
Cuts at: 599 824 2443 599
Size: 225 1619 3563
Fragments arranged by size:
3563 1619 225
Acc65I G'GTAC_C
Cuts at: 1427 2421 1427
Size: 994 4413
AclI AA'CG_TT
Cuts at: 5247 5247
Size: 5407
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1160 1669 1840 2155 2176 3230 3662 4411 4679
Size: 509 171 315 21 1054 432 749 268
Cuts at: 4679 1160
Size: 1888
Fragments arranged by size:
1888 1054 749 509 432 315 268 171
21
AflIII A'CryG_T
Cuts at: 2757 3863 4840 2757
Size: 1106 977 3324
Fragments arranged by size:
3324 1106 977
AgeI A'CCGG_T
Cuts at: 1573 3795 1573
Size: 2222 3185
AhdI GACnn_n'nnGTC
Cuts at: 276 1697 276
Size: 1421 3986
AleI CACnn'nnGTG
Cuts at: 1117 1117
Size: 5407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1135 2092 1135
Size: 957 4450
AlwNI CAG_nnn'CTG
Cuts at: 1662 1776 1860 1986 2710 4279 1662
Size: 114 84 126 724 1569 2790
Fragments arranged by size:
2790 1569 724 126 114 84
ApaI G_GGCC'C
Cuts at: 176 232 448 1449 1498 176
Size: 56 216 1001 49 4085
Fragments arranged by size:
4085 1001 216 56 49
ApaLI G'TGCA_C
Cuts at: 1120 1618 4177 1120
Size: 498 2559 2350
Fragments arranged by size:
2559 2350 498
ApoI r'AATT_y
Cuts at: 2409 4760 5016 2409
Size: 2351 256 2800
Fragments arranged by size:
2800 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5407
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5407
AvaI C'yCGr_G
Cuts at: 153 282 304 351 379 441 511 658 1480
Size: 129 22 47 28 62 70 147 822
Cuts at: 1480 2099 2273 2425 153
Size: 619 174 152 3135
Fragments arranged by size:
3135 822 619 174 152 147 129 70
62 47 28 22
BamHI G'GATC_C
Cuts at: 864 2430 864
Size: 1566 3841
BanI G'GyrC_C
Cuts at: 1216 1427 1949 2209 2421 2835 3083 3118 1216
Size: 211 522 260 212 414 248 35 3505
Fragments arranged by size:
3505 522 414 260 248 212 211 35
BanII G_rGCy'C
Cuts at: 160 176 232 433 448 512 716 878 986
Size: 16 56 201 15 64 204 162 108
Cuts at: 986 1063 1208 1449 1487 1498 1712 2285 2419
Size: 77 145 241 38 11 214 573 134
Cuts at: 2419 3449 160
Size: 1030 2118
Fragments arranged by size:
2118 1030 573 241 214 204 201 162
145 134 108 77 64 56 38 16
15 11
BbeI G_GCGC'C
Cuts at: 2213 2839 3087 2213
Size: 626 248 4533
Fragments arranged by size:
4533 626 248
BbsI GAAGACnn'nnnn_
Cuts at: 597 902 1518 597
Size: 305 616 4486
Fragments arranged by size:
4486 616 305
BbvCI CC'TCA_GC
Cuts at: 685 2197 685
Size: 1512 3895
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1198 1232 1728 1762 1198
Size: 34 496 34 4843
Fragments arranged by size:
4843 496 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3303 4066 5407 3303
Size: 763 1341 3303
Fragments arranged by size:
3303 1341 763
BclI T'GATC_A
Cuts at: 884 2925 884
Size: 2041 3366
BglI GCCn_nnn'nGGC
Cuts at: 142 171 192 201 346 142
Size: 29 21 9 145 5203
Fragments arranged by size:
5203 145 29 21 9
BglII A'GATC_T
Cuts at: 2920 4959 2920
Size: 2039 3368
BlpI GC'TnA_GC
Cuts at: 2517 2517
Size: 5407
Bme1580I G_kGCm'C
Cuts at: 176 232 448 1124 1178 1221 1449 1498 1622
Size: 56 216 676 54 43 228 49 124
Cuts at: 1622 3030 3123 4181 176
Size: 1408 93 1058 1402
Fragments arranged by size:
1408 1402 1058 676 228 216 124 93
56 54 49 43
BmrI ACTGGGnnnn_n'
Cuts at: 863 1382 2617 2698 3033 5179 863
Size: 519 1235 81 335 2146 1091
Fragments arranged by size:
2146 1235 1091 519 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2280 2299 3772 2280
Size: 19 1473 3915
Fragments arranged by size:
3915 1473 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 667 1392 1486 1567 1657 1861 2032 2056
Size: 288 725 94 81 90 204 171 24
Cuts at: 2056 2134 2404 379
Size: 78 270 3382
Fragments arranged by size:
3382 725 288 270 204 171 94 90
81 78 24
Bpu10I CC'TnA_GC
Cuts at: 685 1127 2197 685
Size: 442 1070 3895
Fragments arranged by size:
3895 1070 442
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2014 2581 3460 3954 4252 2014
Size: 567 879 494 298 3169
Fragments arranged by size:
3169 879 567 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 368 1081 1330 1684 2359 5111 368
Size: 713 249 354 675 2752 664
Fragments arranged by size:
2752 713 675 664 354 249
BsaAI yAC'GTr
Cuts at: 2397 2758 2397
Size: 361 5046
BsaBI GATnn'nnATC
Cuts at: 6 2919 4958 6
Size: 2913 2039 455
Fragments arranged by size:
2913 2039 455
BsaHI Gr'CG_yC
Cuts at: 130 437 602 1193 1698 2210 2836 3084 3788
Size: 307 165 591 505 512 626 248 704
Cuts at: 3788 5284 130
Size: 1496 253
Fragments arranged by size:
1496 704 626 591 512 505 307 253
248 165
BsaWI w'CCGG_w
Cuts at: 1573 1799 2594 3115 3795 4069 4216 4627 1573
Size: 226 795 521 680 274 147 411 2353
Fragments arranged by size:
2353 795 680 521 411 274 226 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 242 272 1045 1075 1699 1729 2377 2407 242
Size: 30 773 30 624 30 648 30 3242
Fragments arranged by size:
3242 773 648 624 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 520 529 994 1216 1300 1516 1654 1798 1858
Size: 9 465 222 84 216 138 144 60
Cuts at: 1858 1861 1932 2017 2185 2323 520
Size: 3 71 85 168 138 3604
Fragments arranged by size:
3604 465 222 216 168 144 138 138
85 84 71 60 9 3
BseYI C'CCAG_C
Cuts at: 1356 2830 3588 4167 4663 1356
Size: 1474 758 579 496 2100
Fragments arranged by size:
2100 1474 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1417 1791 1417
Size: 374 5033
BsiEI CG_ry'CG
Cuts at: 104 1172 2947 2993 4203 104
Size: 1068 1775 46 1210 1308
Fragments arranged by size:
1775 1308 1210 1068 46
BsiHKAI G_wGCw'C
Cuts at: 1072 1124 1292 1487 1622 1712 2018 2285 2419
Size: 52 168 195 135 90 306 267 134
Cuts at: 2419 3197 3387 4181 1072
Size: 778 190 794 2298
Fragments arranged by size:
2298 794 778 306 267 195 190 168
135 134 90 52
BsmBI CGTCTCn'nnnn_
Cuts at: 2274 2274
Size: 5407
Bsp1286I G_dGCh'C
Cuts at: 160 176 232 433 448 512 716 878 986
Size: 16 56 201 15 64 204 162 108
Cuts at: 986 1063 1072 1124 1178 1208 1221 1292 1449
Size: 77 9 52 54 30 13 71 157
Cuts at: 1449 1487 1498 1622 1712 2018 2285 2419 3030
Size: 38 11 124 90 306 267 134 611
Cuts at: 3030 3123 3197 3387 3449 4181 160
Size: 93 74 190 62 732 1386
Fragments arranged by size:
1386 732 611 306 267 204 201 190
162 157 134 124 108 93 90 77
74 71 64 62 56 54 52 38
30 16 15 13 11 9
BspEI T'CCGG_A
Cuts at: 4627 4627
Size: 5407
BspHI T'CATG_A
Cuts at: 4583 4583
Size: 5407
BspMI ACCTGCnnnn'nnnn_
Cuts at: 828 1439 1616 1763 2027 2455 2669 2971 3352
Size: 611 177 147 264 428 214 302 381
Cuts at: 3352 828
Size: 2883
Fragments arranged by size:
2883 611 428 381 302 264 214 177
147
BsrBI CCG'CTC
Cuts at: 50 3697 3751 5237 5402 50
Size: 3647 54 1486 165 55
Fragments arranged by size:
3647 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1508 3317 1508
Size: 1809 3598
BsrFI r'CCGG_y
Cuts at: 141 345 1159 1573 3403 3584 3795 141
Size: 204 814 414 1830 181 211 1753
Fragments arranged by size:
1830 1753 814 414 211 204 181
BssHII G'CGCG_C
Cuts at: 545 545
Size: 5407
BssSI C'ACGA_G
Cuts at: 1942 3676 4036 1942
Size: 1734 360 3313
Fragments arranged by size:
3313 1734 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1156 1617 2782 1156
Size: 461 1165 3781
Fragments arranged by size:
3781 1165 461
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5407
BstXI CCAn_nnnn'nTGG
Cuts at: 1116 1116
Size: 5407
BstYI r'GATC_y
Cuts at: 1 864 933 2430 2897 2920 3255 3501 4515
Size: 863 69 1497 467 23 335 246 1014
Cuts at: 4515 4601 4613 4959 1
Size: 86 12 346 449
Fragments arranged by size:
1497 1014 863 467 449 346 335 246
86 69 23 12
Bsu36I CC'TnA_GG
Cuts at: 1594 1594
Size: 5407
BtgI C'CryG_G
Cuts at: 125 412 626 716 1196 1212 1251 1454 125
Size: 287 214 90 480 16 39 203 4078
Fragments arranged by size:
4078 480 287 214 203 90 39 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3355 3536 3699 3355
Size: 181 163 5063
Fragments arranged by size:
5063 181 163
BtsI GCAGTG_nn'
Cuts at: 1320 1371 2648 1320
Size: 51 1277 4079
Fragments arranged by size:
4079 1277 51
DraI TTT'AAA
Cuts at: 2404 2404
Size: 5407
DrdI GACnn_nn'nnGTC
Cuts at: 787 1522 2373 3111 3971 787
Size: 735 851 738 860 2223
Fragments arranged by size:
2223 860 851 738 735
EaeI y'GGCC_r
Cuts at: 942 1169 1254 2354 2990 3164 3555 3582 4929
Size: 227 85 1100 636 174 391 27 1347
Cuts at: 4929 942
Size: 1420
Fragments arranged by size:
1420 1347 1100 636 391 227 174 85
27
EagI C'GGCC_G
Cuts at: 1169 2990 1169
Size: 1821 3586
EarI CTCTTCn'nnn_
Cuts at: 1682 1729 1895 2227 3428 3638 1682
Size: 47 166 332 1201 210 3451
Fragments arranged by size:
3451 1201 332 210 166 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 378 1336 2311 3613 3925 4071 5217 378
Size: 958 975 1302 312 146 1146 568
Fragments arranged by size:
1302 1146 975 958 568 312 146
EcoICRI GAG'CTC
Cuts at: 1485 1710 2283 2417 1485
Size: 225 573 134 4475
Fragments arranged by size:
4475 573 225 134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 667 1160 1392 1486 1567 1657 1669 1840
Size: 288 493 232 94 81 90 12 171
Cuts at: 1840 1861 2032 2056 2134 2155 2176 2404 3230
Size: 21 171 24 78 21 21 228 826
Cuts at: 3230 3662 4411 4679 379
Size: 432 749 268 1107
Fragments arranged by size:
1107 826 749 493 432 288 268 232
228 171 171 94 90 81 78 24
21 21 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1597 1597
Size: 5407
EcoO109I rG'GnC_Cy
Cuts at: 172 229 655 662 1445 1446 1461 1470 1494
Size: 57 426 7 783 1 15 9 24
Cuts at: 1494 1995 2544 172
Size: 501 549 3035
Fragments arranged by size:
3035 783 549 501 426 57 24 15
9 7 1
EcoRI G'AATT_C
Cuts at: 2409 2409
Size: 5407
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 487 1542 2238 3424 487
Size: 1055 696 1186 2470
Fragments arranged by size:
2470 1186 1055 696
FseI GG_CCGG'CC
Cuts at: 349 349
Size: 5407
FspI TGC'GCA
Cuts at: 3186 3186
Size: 5407
HaeII r_GCGC'y
Cuts at: 256 341 469 646 2213 2839 3087 4111 5090
Size: 85 128 177 1567 626 248 1024 979
Cuts at: 5090 256
Size: 573
Fragments arranged by size:
1567 1024 979 626 573 248 177 128
85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 242 262 274 294 592 624 1683 1715 1861
Size: 20 12 20 298 32 1059 32 146
Cuts at: 1861 1893 3247 3277 3279 3309 242
Size: 32 1354 30 2 30 2340
Fragments arranged by size:
2340 1354 1059 298 146 32 32 32
30 30 20 20 12 2
HincII GTy'rAC
Cuts at: 600 2444 600
Size: 1844 3563
KasI G'GCGC_C
Cuts at: 2209 2835 3083 2209
Size: 626 248 4533
Fragments arranged by size:
4533 626 248
KpnI G_GTAC'C
Cuts at: 1431 2425 1431
Size: 994 4413
MluI A'CGCG_T
Cuts at: 4840 4840
Size: 5407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 313 1417 1844 4078 4262 313
Size: 1104 427 2234 184 1458
Fragments arranged by size:
2234 1458 1104 427 184
MscI TGG'CCA
Cuts at: 944 1256 2356 3166 944
Size: 312 1100 810 3185
Fragments arranged by size:
3185 1100 810 312
MslI CAynn'nnrTG
Cuts at: 1117 1250 1270 3394 3521 1117
Size: 133 20 2124 127 3003
Fragments arranged by size:
3003 2124 133 127 20
MspA1I CmG'CkG
Cuts at: 313 414 1356 1614 1647 2010 2049 2516 2830
Size: 101 942 258 33 363 39 467 314
Cuts at: 2830 3190 4205 4450 313
Size: 360 1015 245 1270
Fragments arranged by size:
1270 1015 942 467 363 360 314 258
245 101 39 33
NaeI GCC'GGC
Cuts at: 143 347 1161 3586 143
Size: 204 814 2425 1964
Fragments arranged by size:
2425 1964 814 204
NarI GG'CG_CC
Cuts at: 2210 2836 3084 2210
Size: 626 248 4533
Fragments arranged by size:
4533 626 248
NcoI C'CATG_G
Cuts at: 125 1196 1212 1251 1454 125
Size: 1071 16 39 203 4078
Fragments arranged by size:
4078 1071 203 39 16
NgoMIV G'CCGG_C
Cuts at: 141 345 1159 3584 141
Size: 204 814 2425 1964
Fragments arranged by size:
2425 1964 814 204
NruI TCG'CGA
Cuts at: 3768 3768
Size: 5407
NspI r_CATG'y
Cuts at: 2458 2958 3867 2458
Size: 500 909 3998
Fragments arranged by size:
3998 909 500
PasI CC'CwG_GG
Cuts at: 448 448
Size: 5407
PciI A'CATG_T
Cuts at: 3863 3863
Size: 5407
PfoI T'CCnGG_A
Cuts at: 496 1529 1682 2381 496
Size: 1033 153 699 3522
Fragments arranged by size:
3522 1033 699 153
PmeI GTTT'AAAC
Cuts at: 2404 2404
Size: 5407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4546 4578 4546
Size: 32 5375
PpuMI rG'GwC_Cy
Cuts at: 655 662 1470 1995 655
Size: 7 808 525 4067
Fragments arranged by size:
4067 808 525 7
PspOMI G'GGCC_C
Cuts at: 172 228 444 1445 1494 172
Size: 56 216 1001 49 4085
Fragments arranged by size:
4085 1001 216 56 49
PstI C_TGCA'G
Cuts at: 318 1366 1613 1760 2024 2081 2256 2330 2452
Size: 1048 247 147 264 57 175 74 122
Cuts at: 2452 3137 318
Size: 685 2588
Fragments arranged by size:
2588 1048 685 264 247 175 147 122
74 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5407
PvuII CAG'CTG
Cuts at: 1356 1614 1647 2010 2830 3190 1356
Size: 258 33 363 820 360 3573
Fragments arranged by size:
3573 820 363 360 258 33
RsrII CG'GwC_CG
Cuts at: 3600 3600
Size: 5407
SacI G_AGCT'C
Cuts at: 1487 1712 2285 2419 1487
Size: 225 573 134 4475
Fragments arranged by size:
4475 573 225 134
SacII CC_GC'GG
Cuts at: 415 415
Size: 5407
SalI G'TCGA_C
Cuts at: 598 2442 598
Size: 1844 3563
SanDI GG'GwC_CC
Cuts at: 662 662
Size: 5407
SapI GCTCTTCn'nnn_
Cuts at: 1729 1895 3428 3638 1729
Size: 166 1533 210 3498
Fragments arranged by size:
3498 1533 210 166
SbfI CC_TGCA'GG
Cuts at: 2081 2330 2452 2081
Size: 249 122 5036
Fragments arranged by size:
5036 249 122
ScaI AGT'ACT
Cuts at: 859 4924 859
Size: 4065 1342
SfcI C'TryA_G
Cuts at: 33 314 1074 1362 1609 1756 1768 2020 2077
Size: 281 760 288 247 147 12 252 57
Cuts at: 2077 2252 2326 2448 3133 4128 4319 33
Size: 175 74 122 685 995 191 1121
Fragments arranged by size:
1121 995 760 685 288 281 252 247
191 175 147 122 74 57 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 142 201 142
Size: 59 5348
SfoI GGC'GCC
Cuts at: 2211 2837 3085 2211
Size: 626 248 4533
Fragments arranged by size:
4533 626 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5407
SgrAI Cr'CCGG_yG
Cuts at: 1573 1573
Size: 5407
SmaI CCC'GGG
Cuts at: 155 284 306 353 381 443 660 2101 2427
Size: 129 22 47 28 62 217 1441 326
Cuts at: 2427 155
Size: 3135
Fragments arranged by size:
3135 1441 326 217 129 62 47 28
22
SmlI C'TyrA_G
Cuts at: 2029 2560 3475 3969 4231 2029
Size: 531 915 494 262 3205
Fragments arranged by size:
3205 915 531 494 262
SnaBI TAC'GTA
Cuts at: 2397 2397
Size: 5407
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5407
SphI G_CATG'C
Cuts at: 2458 2958 2458
Size: 500 4907
StuI AGG'CCT
Cuts at: 1242 1242
Size: 5407
StyI C'CwwG_G
Cuts at: 125 906 1196 1212 1251 1454 2539 2893 125
Size: 781 290 16 39 203 1085 354 2639
Fragments arranged by size:
2639 1085 781 354 290 203 39 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1993 2988 3121 3580 3791 1993
Size: 995 133 459 211 3609
Fragments arranged by size:
3609 995 459 211 133
TatI w'GTAC_w
Cuts at: 857 4922 857
Size: 4065 1342
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1374 1406 1699 1731 4924 4956 1374
Size: 32 293 32 3193 32 1825
Fragments arranged by size:
3193 1825 293 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1015 3202 1015
Size: 2187 3220
XbaI T'CTAG_A
Cuts at: 2436 2436
Size: 5407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 952 1416 1461 2900 952
Size: 464 45 1439 3459
Fragments arranged by size:
3459 1439 464 45
XmaI C'CCGG_G
Cuts at: 153 282 304 351 379 441 658 2099 2425
Size: 129 22 47 28 62 217 1441 326
Cuts at: 2425 153
Size: 3135
Fragments arranged by size:
3135 1441 326 217 129 62 47 28
22
ZraI GAC'GTC
Cuts at: 603 603
Size: 5407
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI
PciI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AloI AscI AvrII BaeI BfrBI BmgBI
BmtI BsiWI BsmI BsrGI BstEII BstZ17I ClaI CspCI
DraIII EcoRV FspAI HindIII HpaI MfeI NdeI NheI
NotI NsiI PacI PflMI PmlI PshAI PsiI PspXI
PsrI SexAI SrfI SspI SwaI XhoI XmnI