Restriction map for
pF1KSDA0666
(Circular) (Six-base) MAPSORT of: pF1KSDA0666.seq Check: 3327 from: 1 to: 6373
pF1KSDA0666 6373 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1635 1635
Size: 6373
AccI GT'mk_AC
Cuts at: 3409 3409
Size: 6373
Acc65I G'GTAC_C
Cuts at: 3387 3387
Size: 6373
AclI AA'CG_TT
Cuts at: 6213 6213
Size: 6373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 1939 2919 4196 4628 5377 5645 223
Size: 1716 980 1277 432 749 268 951
Fragments arranged by size:
1716 1277 980 951 749 432 268
AflIII A'CryG_T
Cuts at: 2303 3723 4829 5806 2303
Size: 1420 1106 977 2870
Fragments arranged by size:
2870 1420 1106 977
AgeI A'CCGG_T
Cuts at: 4761 4761
Size: 6373
AleI CACnn'nnGTG
Cuts at: 2713 2713
Size: 6373
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 277 309 2098 2130 277
Size: 32 1789 32 4520
Fragments arranged by size:
4520 1789 32 32
AlwNI CAG_nnn'CTG
Cuts at: 759 1521 2565 3676 5245 759
Size: 762 1044 1111 1569 1887
Fragments arranged by size:
1887 1569 1111 1044 762
ApaI G_GGCC'C
Cuts at: 1742 1742
Size: 6373
ApaLI G'TGCA_C
Cuts at: 5143 5143
Size: 6373
ApoI r'AATT_y
Cuts at: 436 1045 1061 1098 1159 2039 2323 3375 5726
Size: 609 16 37 61 880 284 1052 2351
Cuts at: 5726 5982 436
Size: 256 827
Fragments arranged by size:
2351 1052 880 827 609 284 256 61
37 16
AseI AT'TA_AT
Cuts at: 20 740 904 980 2426 20
Size: 720 164 76 1446 3967
Fragments arranged by size:
3967 1446 720 164 76
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6373
AvaI C'yCGr_G
Cuts at: 826 1981 3391 826
Size: 1155 1410 3808
Fragments arranged by size:
3808 1410 1155
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2015 2048 2015
Size: 33 6340
BamHI G'GATC_C
Cuts at: 3396 3396
Size: 6373
BanI G'GyrC_C
Cuts at: 808 2548 3387 3801 4049 4084 808
Size: 1740 839 414 248 35 3097
Fragments arranged by size:
3097 1740 839 414 248 35
BanII G_rGCy'C
Cuts at: 714 1679 1742 2205 3138 3385 4415 714
Size: 965 63 463 933 247 1030 2672
Fragments arranged by size:
2672 1030 965 933 463 247 63
BbeI G_GCGC'C
Cuts at: 812 3805 4053 812
Size: 2993 248 3132
Fragments arranged by size:
3132 2993 248
BbsI GAAGACnn'nnnn_
Cuts at: 2025 2060 2960 2025
Size: 35 900 5438
Fragments arranged by size:
5438 900 35
BbvCI CC'TCA_GC
Cuts at: 1937 1937
Size: 6373
BciVI GTATCCnnnnn_n'
Cuts at: 616 4269 5032 6373 616
Size: 3653 763 1341 616
Fragments arranged by size:
3653 1341 763 616
BclI T'GATC_A
Cuts at: 189 447 3054 3891 189
Size: 258 2607 837 2671
Fragments arranged by size:
2671 2607 837 258
BfrBI ATG'CAT
Cuts at: 2620 2620
Size: 6373
BglII A'GATC_T
Cuts at: 2046 2289 3886 5925 2046
Size: 243 1597 2039 2494
Fragments arranged by size:
2494 2039 1597 243
BlpI GC'TnA_GC
Cuts at: 991 1678 3149 3483 991
Size: 687 1471 334 3881
Fragments arranged by size:
3881 1471 687 334
Bme1580I G_kGCm'C
Cuts at: 1742 2553 3996 4089 5147 1742
Size: 811 1443 93 1058 2968
Fragments arranged by size:
2968 1443 1058 811 93
BmgBI CAC'GTC
Cuts at: 717 717
Size: 6373
BmrI ACTGGGnnnn_n'
Cuts at: 938 2726 3583 3664 3999 6145 938
Size: 1788 857 81 335 2146 1166
Fragments arranged by size:
2146 1788 1166 857 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4738 4738
Size: 6373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1745 1820 2587 1745
Size: 75 767 5531
Fragments arranged by size:
5531 767 75
Bpu10I CC'TnA_GC
Cuts at: 1937 3249 1937
Size: 1312 5061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 940 1541 2539 2756 2841 3547 4426 4920
Size: 254 601 998 217 85 706 879 494
Cuts at: 4920 5218 686
Size: 298 1841
Fragments arranged by size:
1841 998 879 706 601 494 298 254
217 85
BsaI GGTCTCn'nnnn_
Cuts at: 631 3330 6077 631
Size: 2699 2747 927
Fragments arranged by size:
2747 2699 927
BsaAI yAC'GTr
Cuts at: 206 3363 3724 206
Size: 3157 361 2855
Fragments arranged by size:
3157 2855 361
BsaBI GATnn'nnATC
Cuts at: 6 1363 3885 5924 6
Size: 1357 2522 2039 455
Fragments arranged by size:
2522 2039 1357 455
BsaHI Gr'CG_yC
Cuts at: 809 3802 4050 4754 6250 809
Size: 2993 248 704 1496 932
Fragments arranged by size:
2993 1496 932 704 248
BsaWI w'CCGG_w
Cuts at: 3560 4081 4761 5035 5182 5593 3560
Size: 521 680 274 147 411 4340
Fragments arranged by size:
4340 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1378 1408 2098 2128 1378
Size: 30 690 30 5623
Fragments arranged by size:
5623 690 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 292 1320 1838 1841 1856 292
Size: 1028 518 3 15 4809
Fragments arranged by size:
4809 1028 518 15 3
BseYI C'CCAG_C
Cuts at: 3796 4554 5133 5629 3796
Size: 758 579 496 4540
Fragments arranged by size:
4540 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1022 1261 844
Size: 178 239 5956
Fragments arranged by size:
5956 239 178
BsiEI CG_ry'CG
Cuts at: 104 3913 3959 5169 104
Size: 3809 46 1210 1308
Fragments arranged by size:
3809 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 766 992 1502 1679 1731 1902 2205 3138 3385
Size: 226 510 177 52 171 303 933 247
Cuts at: 3385 4163 4353 5147 766
Size: 778 190 794 1992
Fragments arranged by size:
1992 933 794 778 510 303 247 226
190 177 171 52
BsiWI C'GTAC_G
Cuts at: 1431 1431
Size: 6373
BsmI GAATG_Cn'
Cuts at: 1931 2622 1931
Size: 691 5682
Bsp1286I G_dGCh'C
Cuts at: 714 766 992 1502 1679 1731 1742 1902 2205
Size: 52 226 510 177 52 11 160 303
Cuts at: 2205 2553 3138 3385 3996 4089 4163 4353 4415
Size: 348 585 247 611 93 74 190 62
Cuts at: 4415 5147 714
Size: 732 1940
Fragments arranged by size:
1940 732 611 585 510 348 303 247
226 190 177 160 93 74 62 52
52 11
BspEI T'CCGG_A
Cuts at: 5593 5593
Size: 6373
BspHI T'CATG_A
Cuts at: 5549 5549
Size: 6373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1635 3421 3635 3937 4318 1635
Size: 1786 214 302 381 3690
Fragments arranged by size:
3690 1786 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4663 4717 6203 6368 50
Size: 4613 54 1486 165 55
Fragments arranged by size:
4613 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 256 4283 256
Size: 4027 2346
BsrFI r'CCGG_y
Cuts at: 4369 4550 4761 4369
Size: 181 211 5981
Fragments arranged by size:
5981 211 181
BssSI C'ACGA_G
Cuts at: 4642 5002 4642
Size: 360 6013
BstAPI GCAn_nnn'nTGC
Cuts at: 855 2445 3748 855
Size: 1590 1303 3480
Fragments arranged by size:
3480 1590 1303
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6373
BstXI CCAn_nnnn'nTGG
Cuts at: 1404 1404
Size: 6373
BstYI r'GATC_y
Cuts at: 1 1762 2046 2289 3396 3863 3886 4221 4467
Size: 1761 284 243 1107 467 23 335 246
Cuts at: 4467 5481 5567 5579 5925 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1761 1107 1014 467 449 346 335 284
246 243 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1872 1872
Size: 6373
BtgI C'CryG_G
Cuts at: 125 245 461 626 125
Size: 120 216 165 5872
Fragments arranged by size:
5872 216 165 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4321 4502 4665 4321
Size: 181 163 6029
Fragments arranged by size:
6029 181 163
BtsI GCAGTG_nn'
Cuts at: 992 1281 1471 2999 3614 992
Size: 289 190 1528 615 3751
Fragments arranged by size:
3751 1528 615 289 190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1290 1325 1290
Size: 35 6338
DraI TTT'AAA
Cuts at: 536 3370 536
Size: 2834 3539
DraIII CAC_nnn'GTG
Cuts at: 1246 1246
Size: 6373
DrdI GACnn_nn'nnGTC
Cuts at: 645 4077 4937 645
Size: 3432 860 2081
Fragments arranged by size:
3432 2081 860
EaeI y'GGCC_r
Cuts at: 788 2387 3956 4130 4521 4548 5895 788
Size: 1599 1569 174 391 27 1347 1266
Fragments arranged by size:
1599 1569 1347 1266 391 174 27
EagI C'GGCC_G
Cuts at: 3956 3956
Size: 6373
EarI CTCTTCn'nnn_
Cuts at: 1225 1562 2525 3005 4394 4604 1225
Size: 337 963 480 1389 210 2994
Fragments arranged by size:
2994 1389 963 480 337 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1666 4579 4891 5037 6183 1666
Size: 2913 312 146 1146 1856
Fragments arranged by size:
2913 1856 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1677 2203 3136 3383 1677
Size: 526 933 247 4667
Fragments arranged by size:
4667 933 526 247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 1745 1820 1939 2587 2919 4196 4628 5377
Size: 1522 75 119 648 332 1277 432 749
Cuts at: 5377 5645 223
Size: 268 951
Fragments arranged by size:
1522 1277 951 749 648 432 332 268
119 75
EcoNI CCTnn'n_nnAGG
Cuts at: 1796 1856 1871 2356 1796
Size: 60 15 485 5813
Fragments arranged by size:
5813 485 60 15
EcoO109I rG'GnC_Cy
Cuts at: 887 1386 1738 1739 1861 3510 887
Size: 499 352 1 122 1649 3750
Fragments arranged by size:
3750 1649 499 352 122 1
EcoRI G'AATT_C
Cuts at: 436 1061 3375 436
Size: 625 2314 3434
Fragments arranged by size:
3434 2314 625
EcoRV GAT'ATC
Cuts at: 2636 2636
Size: 6373
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1621 4390 1621
Size: 2769 3604
FspI TGC'GCA
Cuts at: 4152 4152
Size: 6373
HaeII r_GCGC'y
Cuts at: 812 3805 4053 5077 6056 812
Size: 2993 248 1024 979 1129
Fragments arranged by size:
2993 1129 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 583 615 1378 1410 2178 2210 2650 2682 4213
Size: 32 763 32 768 32 440 32 1531
Cuts at: 4213 4243 4245 4275 583
Size: 30 2 30 2681
Fragments arranged by size:
2681 1531 768 763 440 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3410 3410
Size: 6373
HindIII A'AGCT_T
Cuts at: 1250 1250
Size: 6373
KasI G'GCGC_C
Cuts at: 808 3801 4049 808
Size: 2993 248 3132
Fragments arranged by size:
3132 2993 248
KpnI G_GTAC'C
Cuts at: 3391 3391
Size: 6373
MluI A'CGCG_T
Cuts at: 5806 5806
Size: 6373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2176 2288 5044 5228 2176
Size: 112 2756 184 3321
Fragments arranged by size:
3321 2756 184 112
MscI TGG'CCA
Cuts at: 2389 4132 2389
Size: 1743 4630
MslI CAynn'nnrTG
Cuts at: 2713 4360 4487 2713
Size: 1647 127 4599
Fragments arranged by size:
4599 1647 127
MspA1I CmG'CkG
Cuts at: 361 3482 3796 4156 5171 5416 361
Size: 3121 314 360 1015 245 1318
Fragments arranged by size:
3121 1318 1015 360 314 245
NaeI GCC'GGC
Cuts at: 4552 4552
Size: 6373
NarI GG'CG_CC
Cuts at: 809 3802 4050 809
Size: 2993 248 3132
Fragments arranged by size:
3132 2993 248
NcoI C'CATG_G
Cuts at: 125 245 461 626 125
Size: 120 216 165 5872
Fragments arranged by size:
5872 216 165 120
NdeI CA'TA_TG
Cuts at: 2622 2622
Size: 6373
NgoMIV G'CCGG_C
Cuts at: 4550 4550
Size: 6373
NruI TCG'CGA
Cuts at: 4734 4734
Size: 6373
NsiI A_TGCA'T
Cuts at: 2622 2622
Size: 6373
NspI r_CATG'y
Cuts at: 2307 3424 3924 4833 2307
Size: 1117 500 909 3847
Fragments arranged by size:
3847 1117 909 500
PacI TTA_AT'TAA
Cuts at: 908 908
Size: 6373
PasI CC'CwG_GG
Cuts at: 1858 1858
Size: 6373
PciI A'CATG_T
Cuts at: 2303 4829 2303
Size: 2526 3847
PflMI CCAn_nnn'nTGG
Cuts at: 1894 1894
Size: 6373
PmeI GTTT'AAAC
Cuts at: 3370 3370
Size: 6373
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 305 337 2098 2130 5512 5544 305
Size: 32 1761 32 3382 32 1134
Fragments arranged by size:
3382 1761 1134 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 887 1386 887
Size: 499 5874
PspOMI G'GGCC_C
Cuts at: 1738 1738
Size: 6373
PstI C_TGCA'G
Cuts at: 851 3418 4103 851
Size: 2567 685 3121
Fragments arranged by size:
3121 2567 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6373
PvuII CAG'CTG
Cuts at: 361 3796 4156 361
Size: 3435 360 2578
Fragments arranged by size:
3435 2578 360
RsrII CG'GwC_CG
Cuts at: 4566 4566
Size: 6373
SacI G_AGCT'C
Cuts at: 1679 2205 3138 3385 1679
Size: 526 933 247 4667
Fragments arranged by size:
4667 933 526 247
SalI G'TCGA_C
Cuts at: 3408 3408
Size: 6373
SapI GCTCTTCn'nnn_
Cuts at: 4394 4604 4394
Size: 210 6163
SbfI CC_TGCA'GG
Cuts at: 3418 3418
Size: 6373
ScaI AGT'ACT
Cuts at: 1327 5890 1327
Size: 4563 1810
SexAI A'CCwGG_T
Cuts at: 1797 1797
Size: 6373
SfcI C'TryA_G
Cuts at: 33 384 524 847 3414 4099 5094 5285 33
Size: 351 140 323 2567 685 995 191 1121
Fragments arranged by size:
2567 1121 995 685 351 323 191 140
SfoI GGC'GCC
Cuts at: 810 3803 4051 810
Size: 2993 248 3132
Fragments arranged by size:
3132 2993 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6373
SmaI CCC'GGG
Cuts at: 828 3393 828
Size: 2565 3808
SmlI C'TyrA_G
Cuts at: 701 955 1556 2554 2771 2820 3526 4441 4935
Size: 254 601 998 217 49 706 915 494
Cuts at: 4935 5197 701
Size: 262 1877
Fragments arranged by size:
1877 998 915 706 601 494 262 254
217 49
SnaBI TAC'GTA
Cuts at: 3363 3363
Size: 6373
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6373
SphI G_CATG'C
Cuts at: 3424 3924 3424
Size: 500 5873
SspI AAT'ATT
Cuts at: 1419 1419
Size: 6373
StyI C'CwwG_G
Cuts at: 125 245 461 626 996 2297 2390 3505 3859
Size: 120 216 165 370 1301 93 1115 354
Cuts at: 3859 125
Size: 2639
Fragments arranged by size:
2639 1301 1115 370 354 216 165 120
93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 209 2026 3954 4087 4546 4757 209
Size: 1817 1928 133 459 211 1825
Fragments arranged by size:
1928 1825 1817 459 211 133
TatI w'GTAC_w
Cuts at: 1325 2456 5888 1325
Size: 1131 3432 1810
Fragments arranged by size:
3432 1810 1131
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5890 5922 5890
Size: 32 6341
Tth111I GACn'n_nGTC
Cuts at: 4168 4168
Size: 6373
XbaI T'CTAG_A
Cuts at: 2043 3402 2043
Size: 1359 5014
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 270 2304 3866 270
Size: 2034 1562 2777
Fragments arranged by size:
2777 2034 1562
XmaI C'CCGG_G
Cuts at: 826 3391 826
Size: 2565 3808
XmnI GAAnn'nnTTC
Cuts at: 1181 1960 2513 2957 1181
Size: 779 553 444 4597
Fragments arranged by size:
4597 779 553 444
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BcgI
BglI BmtI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
FseI FspAI HpaI MfeI NheI NotI PfoI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SrfI StuI SwaI XhoI ZraI