Restriction map for
pF1KSDA0677
(Circular) (Six-base) MAPSORT of: pF1KSDA0677.seq Check: 7135 from: 1 to: 6331
pF1KSDA0677 6331 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 988 3134 3367 988
Size: 2146 233 3952
Fragments arranged by size:
3952 2146 233
Acc65I G'GTAC_C
Cuts at: 538 3345 538
Size: 2807 3524
AclI AA'CG_TT
Cuts at: 6171 6171
Size: 6331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 713 895 1300 1526 2186 2420 2919 3020
Size: 524 182 405 226 660 234 499 101
Cuts at: 3020 4154 4586 5335 5603 189
Size: 1134 432 749 268 917
Fragments arranged by size:
1134 917 749 660 524 499 432 405
268 234 226 182 101
AflII C'TTAA_G
Cuts at: 1186 1186
Size: 6331
AflIII A'CryG_T
Cuts at: 1874 3681 4787 5764 1874
Size: 1807 1106 977 2441
Fragments arranged by size:
2441 1807 1106 977
AgeI A'CCGG_T
Cuts at: 4719 4719
Size: 6331
AhdI GACnn_n'nnGTC
Cuts at: 1621 1621
Size: 6331
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1781 1781
Size: 6331
AlwNI CAG_nnn'CTG
Cuts at: 3634 5203 3634
Size: 1569 4762
ApaI G_GGCC'C
Cuts at: 2478 2478
Size: 6331
ApaLI G'TGCA_C
Cuts at: 1756 5101 1756
Size: 3345 2986
ApoI r'AATT_y
Cuts at: 887 1513 2092 2167 3333 5684 5940 887
Size: 626 579 75 1166 2351 256 1278
Fragments arranged by size:
2351 1278 1166 626 579 256 75
AseI AT'TA_AT
Cuts at: 20 20
Size: 6331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6331
AvaI C'yCGr_G
Cuts at: 475 3349 475
Size: 2874 3457
BamHI G'GATC_C
Cuts at: 3354 3354
Size: 6331
BanI G'GyrC_C
Cuts at: 538 1319 2317 3345 3759 4007 4042 538
Size: 781 998 1028 414 248 35 2827
Fragments arranged by size:
2827 1028 998 781 414 248 35
BanII G_rGCy'C
Cuts at: 243 476 1367 1666 1731 2478 3343 4373 243
Size: 233 891 299 65 747 865 1030 2201
Fragments arranged by size:
2201 1030 891 865 747 299 233 65
BbeI G_GCGC'C
Cuts at: 3763 4011 3763
Size: 248 6083
BbsI GAAGACnn'nnnn_
Cuts at: 676 701 1288 1617 2408 2748 676
Size: 25 587 329 791 340 4259
Fragments arranged by size:
4259 791 587 340 329 25
BbvCI CC'TCA_GC
Cuts at: 2015 2015
Size: 6331
BciVI GTATCCnnnnn_n'
Cuts at: 4227 4990 6331 4227
Size: 763 1341 4227
Fragments arranged by size:
4227 1341 763
BclI T'GATC_A
Cuts at: 929 3849 929
Size: 2920 3411
BglI GCCn_nnn'nGGC
Cuts at: 2116 2806 2116
Size: 690 5641
BglII A'GATC_T
Cuts at: 1067 3844 5883 1067
Size: 2777 2039 1515
Fragments arranged by size:
2777 2039 1515
BlpI GC'TnA_GC
Cuts at: 3441 3441
Size: 6331
Bme1580I G_kGCm'C
Cuts at: 1234 1417 1760 2478 3954 4047 5105 1234
Size: 183 343 718 1476 93 1058 2460
Fragments arranged by size:
2460 1476 1058 718 343 183 93
BmrI ACTGGGnnnn_n'
Cuts at: 149 3541 3622 3957 6103 149
Size: 3392 81 335 2146 377
Fragments arranged by size:
3392 2146 377 335 81
BmtI G_CTAG'C
Cuts at: 259 259
Size: 6331
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1734 3120 4696 1734
Size: 1386 1576 3369
Fragments arranged by size:
3369 1576 1386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 938 2965 938
Size: 2027 4304
Bpu10I CC'TnA_GC
Cuts at: 764 2015 764
Size: 1251 5080
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 893 2326 3249 3505 4384 4878 5176 893
Size: 1433 923 256 879 494 298 2048
Fragments arranged by size:
2048 1433 923 879 494 298 256
BsaI GGTCTCn'nnnn_
Cuts at: 1268 2095 2893 6035 1268
Size: 827 798 3142 1564
Fragments arranged by size:
3142 1564 827 798
BsaAI yAC'GTr
Cuts at: 3321 3682 3321
Size: 361 5970
BsaBI GATnn'nnATC
Cuts at: 6 3843 5882 6
Size: 3837 2039 455
Fragments arranged by size:
3837 2039 455
BsaHI Gr'CG_yC
Cuts at: 1617 1911 3760 4008 4712 6208 1617
Size: 294 1849 248 704 1496 1740
Fragments arranged by size:
1849 1740 1496 704 294 248
BsaWI w'CCGG_w
Cuts at: 2568 3518 4039 4719 4993 5140 5551 2568
Size: 950 521 680 274 147 411 3348
Fragments arranged by size:
3348 950 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1237 1240 1252 1561 1570 2032 2323 2455 2483
Size: 3 12 309 9 462 291 132 28
Cuts at: 2483 1237
Size: 5085
Fragments arranged by size:
5085 462 309 291 132 28 12 9
3
BseYI C'CCAG_C
Cuts at: 1126 3754 4512 5091 5587 1126
Size: 2628 758 579 496 1870
Fragments arranged by size:
2628 1870 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 709 2367 2680 3278 545
Size: 164 1658 313 598 3598
Fragments arranged by size:
3598 1658 598 313 164
BsiEI CG_ry'CG
Cuts at: 104 2676 3871 3917 5127 104
Size: 2572 1195 46 1210 1308
Fragments arranged by size:
2572 1308 1210 1195 46
BsiHKAI G_wGCw'C
Cuts at: 243 476 819 1045 1367 1760 3343 4121 4311
Size: 233 343 226 322 393 1583 778 190
Cuts at: 4311 5105 243
Size: 794 1469
Fragments arranged by size:
1583 1469 794 778 393 343 322 233
226 190
Bsp1286I G_dGCh'C
Cuts at: 243 476 819 1045 1234 1367 1417 1666 1731
Size: 233 343 226 189 133 50 249 65
Cuts at: 1731 1760 2478 3343 3954 4047 4121 4311 4373
Size: 29 718 865 611 93 74 190 62
Cuts at: 4373 5105 243
Size: 732 1469
Fragments arranged by size:
1469 865 732 718 611 343 249 233
226 190 189 133 93 74 65 62
50 29
BspEI T'CCGG_A
Cuts at: 5551 5551
Size: 6331
BspHI T'CATG_A
Cuts at: 5507 5507
Size: 6331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 731 2773 3379 3593 3895 4276 731
Size: 2042 606 214 302 381 2786
Fragments arranged by size:
2786 2042 606 381 302 214
BsrBI CCG'CTC
Cuts at: 50 480 1845 4621 4675 6161 6326 50
Size: 430 1365 2776 54 1486 165 55
Fragments arranged by size:
2776 1486 1365 430 165 55 54
BsrDI GCAATG_nn'
Cuts at: 215 2552 4241 215
Size: 2337 1689 2305
Fragments arranged by size:
2337 2305 1689
BsrFI r'CCGG_y
Cuts at: 578 955 2716 4327 4508 4719 578
Size: 377 1761 1611 181 211 2190
Fragments arranged by size:
2190 1761 1611 377 211 181
BssSI C'ACGA_G
Cuts at: 290 1806 4600 4960 290
Size: 1516 2794 360 1661
Fragments arranged by size:
2794 1661 1516 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1035 1861 2345 3706 1035
Size: 826 484 1361 3660
Fragments arranged by size:
3660 1361 826 484
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6331
BstEII G'GTnAC_C
Cuts at: 946 946
Size: 6331
BstXI CCAn_nnnn'nTGG
Cuts at: 281 746 3043 3057 281
Size: 465 2297 14 3555
Fragments arranged by size:
3555 2297 465 14
BstYI r'GATC_y
Cuts at: 1 1067 1380 1584 3354 3821 3844 4179 4425
Size: 1066 313 204 1770 467 23 335 246
Cuts at: 4425 5439 5525 5537 5883 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1770 1066 1014 467 449 346 335 313
246 204 86 23 12
BstZ17I GTA'TAC
Cuts at: 3135 3135
Size: 6331
Bsu36I CC'TnA_GG
Cuts at: 2003 2954 2003
Size: 951 5380
BtgI C'CryG_G
Cuts at: 125 966 1073 2105 125
Size: 841 107 1032 4351
Fragments arranged by size:
4351 1032 841 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4279 4460 4623 4279
Size: 181 163 5987
Fragments arranged by size:
5987 181 163
BtsI GCAGTG_nn'
Cuts at: 2124 2668 2822 3572 2124
Size: 544 154 750 4883
Fragments arranged by size:
4883 750 544 154
DraI TTT'AAA
Cuts at: 3328 3328
Size: 6331
DrdI GACnn_nn'nnGTC
Cuts at: 357 2503 3037 4035 4895 357
Size: 2146 534 998 860 1793
Fragments arranged by size:
2146 1793 998 860 534
EaeI y'GGCC_r
Cuts at: 576 1292 2673 3033 3914 4088 4479 4506 5853
Size: 716 1381 360 881 174 391 27 1347
Cuts at: 5853 576
Size: 1054
Fragments arranged by size:
1381 1347 1054 881 716 391 360 174
27
EagI C'GGCC_G
Cuts at: 2673 3914 2673
Size: 1241 5090
EarI CTCTTCn'nnn_
Cuts at: 185 464 1372 1514 1538 1677 1695 1883 2003
Size: 279 908 142 24 139 18 188 120
Cuts at: 2003 3143 4352 4562 185
Size: 1140 1209 210 1954
Fragments arranged by size:
1954 1209 1140 908 279 210 188 142
139 120 24 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 829 4537 4849 4995 6141 829
Size: 3708 312 146 1146 1019
Fragments arranged by size:
3708 1146 1019 312 146
EcoICRI GAG'CTC
Cuts at: 241 474 1365 3341 241
Size: 233 891 1976 3231
Fragments arranged by size:
3231 1976 891 233
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 713 895 938 1300 1526 2186 2420 2919
Size: 524 182 43 362 226 660 234 499
Cuts at: 2919 2965 3020 4154 4586 5335 5603 189
Size: 46 55 1134 432 749 268 917
Fragments arranged by size:
1134 917 749 660 524 499 432 362
268 234 226 182 55 46 43
EcoNI CCTnn'n_nnAGG
Cuts at: 2440 2999 2440
Size: 559 5772
EcoO109I rG'GnC_Cy
Cuts at: 2118 2379 2474 2475 2807 3468 2118
Size: 261 95 1 332 661 4981
Fragments arranged by size:
4981 661 332 261 95 1
EcoRI G'AATT_C
Cuts at: 887 2092 3333 887
Size: 1205 1241 3885
Fragments arranged by size:
3885 1241 1205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 693 2826 4348 693
Size: 2133 1522 2676
Fragments arranged by size:
2676 2133 1522
FspI TGC'GCA
Cuts at: 4110 4110
Size: 6331
HaeII r_GCGC'y
Cuts at: 1826 2865 3763 4011 5035 6014 1826
Size: 1039 898 248 1024 979 2143
Fragments arranged by size:
2143 1039 1024 979 898 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 162 194 1607 1639 1672 1704 1969 2001 2142
Size: 32 1413 32 33 32 265 32 141
Cuts at: 2142 2174 2400 2432 2914 2946 4171 4201 4203
Size: 32 226 32 482 32 1225 30 2
Cuts at: 4203 4233 162
Size: 30 2260
Fragments arranged by size:
2260 1413 1225 482 265 226 141 33
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2550 2916 3368 2550
Size: 366 452 5513
Fragments arranged by size:
5513 452 366
KasI G'GCGC_C
Cuts at: 3759 4007 3759
Size: 248 6083
KpnI G_GTAC'C
Cuts at: 542 3349 542
Size: 2807 3524
MluI A'CGCG_T
Cuts at: 5764 5764
Size: 6331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 205 761 5002 5186 205
Size: 556 4241 184 1350
Fragments arranged by size:
4241 1350 556 184
MscI TGG'CCA
Cuts at: 1294 4090 1294
Size: 2796 3535
MslI CAynn'nnrTG
Cuts at: 560 1078 1763 4318 4445 560
Size: 518 685 2555 127 2446
Fragments arranged by size:
2555 2446 685 518 127
MspA1I CmG'CkG
Cuts at: 345 1177 1576 1995 2678 3440 3754 4114 5129
Size: 832 399 419 683 762 314 360 1015
Cuts at: 5129 5374 345
Size: 245 1302
Fragments arranged by size:
1302 1015 832 762 683 419 399 360
314 245
NaeI GCC'GGC
Cuts at: 580 957 4510 580
Size: 377 3553 2401
Fragments arranged by size:
3553 2401 377
NarI GG'CG_CC
Cuts at: 3760 4008 3760
Size: 248 6083
NcoI C'CATG_G
Cuts at: 125 966 1073 2105 125
Size: 841 107 1032 4351
Fragments arranged by size:
4351 1032 841 107
NgoMIV G'CCGG_C
Cuts at: 578 955 4508 578
Size: 377 3553 2401
Fragments arranged by size:
3553 2401 377
NheI G'CTAG_C
Cuts at: 255 255
Size: 6331
NruI TCG'CGA
Cuts at: 4692 4692
Size: 6331
NspI r_CATG'y
Cuts at: 559 666 1878 3198 3382 3882 4791 559
Size: 107 1212 1320 184 500 909 2099
Fragments arranged by size:
2099 1320 1212 909 500 184 107
PciI A'CATG_T
Cuts at: 1874 4787 1874
Size: 2913 3418
PflMI CCAn_nnn'nTGG
Cuts at: 520 1213 520
Size: 693 5638
PfoI T'CCnGG_A
Cuts at: 593 593
Size: 6331
PmeI GTTT'AAAC
Cuts at: 3328 3328
Size: 6331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5470 5502 5470
Size: 32 6299
PpuMI rG'GwC_Cy
Cuts at: 2379 2379
Size: 6331
PspOMI G'GGCC_C
Cuts at: 2474 2474
Size: 6331
PspXI vC'TCGA_Gb
Cuts at: 475 475
Size: 6331
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 631 663 631
Size: 32 6299
PstI C_TGCA'G
Cuts at: 1961 2275 2483 2829 3376 4061 1961
Size: 314 208 346 547 685 4231
Fragments arranged by size:
4231 685 547 346 314 208
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6331
PvuII CAG'CTG
Cuts at: 345 1177 1576 1995 3754 4114 345
Size: 832 399 419 1759 360 2562
Fragments arranged by size:
2562 1759 832 419 399 360
RsrII CG'GwC_CG
Cuts at: 4524 4524
Size: 6331
SacI G_AGCT'C
Cuts at: 243 476 1367 3343 243
Size: 233 891 1976 3231
Fragments arranged by size:
3231 1976 891 233
SalI G'TCGA_C
Cuts at: 3366 3366
Size: 6331
SanDI GG'GwC_CC
Cuts at: 2379 2379
Size: 6331
SapI GCTCTTCn'nnn_
Cuts at: 464 1372 1514 1677 3143 4352 4562 464
Size: 908 142 163 1466 1209 210 2233
Fragments arranged by size:
2233 1466 1209 908 210 163 142
SbfI CC_TGCA'GG
Cuts at: 3376 3376
Size: 6331
ScaI AGT'ACT
Cuts at: 442 5848 442
Size: 5406 925
SexAI A'CCwGG_T
Cuts at: 3083 3083
Size: 6331
SfcI C'TryA_G
Cuts at: 33 456 708 1047 1607 1957 2271 2479 2825
Size: 423 252 339 560 350 314 208 346
Cuts at: 2825 3372 4057 5052 5243 33
Size: 547 685 995 191 1121
Fragments arranged by size:
1121 995 685 560 547 423 350 346
339 314 252 208 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2116 2116
Size: 6331
SfoI GGC'GCC
Cuts at: 3761 4009 3761
Size: 248 6083
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6331
SmaI CCC'GGG
Cuts at: 3351 3351
Size: 6331
SmlI C'TyrA_G
Cuts at: 475 908 1186 2305 3264 3484 4399 4893 5155
Size: 433 278 1119 959 220 915 494 262
Cuts at: 5155 475
Size: 1651
Fragments arranged by size:
1651 1119 959 915 494 433 278 262
220
SnaBI TAC'GTA
Cuts at: 3321 3321
Size: 6331
SpeI A'CTAG_T
Cuts at: 67 1705 67
Size: 1638 4693
SphI G_CATG'C
Cuts at: 3382 3882 3382
Size: 500 5831
SspI AAT'ATT
Cuts at: 573 573
Size: 6331
StuI AGG'CCT
Cuts at: 2029 2029
Size: 6331
StyI C'CwwG_G
Cuts at: 125 234 260 966 1073 1373 1580 1731 1904
Size: 109 26 706 107 300 207 151 173
Cuts at: 1904 2105 2801 3463 3817 125
Size: 201 696 662 354 2639
Fragments arranged by size:
2639 706 696 662 354 300 207 201
173 151 109 107 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1459 1961 3912 4045 4504 4715 1459
Size: 502 1951 133 459 211 3075
Fragments arranged by size:
3075 1951 502 459 211 133
TatI w'GTAC_w
Cuts at: 377 440 653 5846 377
Size: 63 213 5193 862
Fragments arranged by size:
5193 862 213 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5848 5880 5848
Size: 32 6299
Tth111I GACn'n_nGTC
Cuts at: 1913 4126 1913
Size: 2213 4118
XbaI T'CTAG_A
Cuts at: 3172 3360 3172
Size: 188 6143
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 342 3824 342
Size: 3482 2849
XhoI C'TCGA_G
Cuts at: 475 475
Size: 6331
XmaI C'CCGG_G
Cuts at: 3349 3349
Size: 6331
XmnI GAAnn'nnTTC
Cuts at: 2092 2199 3152 2092
Size: 107 953 5271
Fragments arranged by size:
5271 953 107
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NruI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AleI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BsaXI BsiWI BsmI BsmBI BsrGI
BssHII ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NotI NsiI PacI PasI PmlI
PshAI PsiI SacII SgrAI SrfI SwaI ZraI