Restriction map for
pF1KSDA0693
(Circular) (Six-base) MAPSORT of: pF1KSDA0693.seq Check: 2100 from: 1 to: 4327
pF1KSDA0693 4327 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 302 1363 302
Size: 1061 3266
Acc65I G'GTAC_C
Cuts at: 1073 1341 1073
Size: 268 4059
AclI AA'CG_TT
Cuts at: 4167 4167
Size: 4327
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2150 2582 3331 3599 2150
Size: 432 749 268 2878
Fragments arranged by size:
2878 749 432 268
AflIII A'CryG_T
Cuts at: 512 644 1677 2783 3760 512
Size: 132 1033 1106 977 1079
Fragments arranged by size:
1106 1079 1033 977 132
AgeI A'CCGG_T
Cuts at: 1001 2715 1001
Size: 1714 2613
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 478 478
Size: 4327
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 325 357 325
Size: 32 4295
AlwNI CAG_nnn'CTG
Cuts at: 285 345 420 864 1630 3199 285
Size: 60 75 444 766 1569 1413
Fragments arranged by size:
1569 1413 766 444 75 60
ApaI G_GGCC'C
Cuts at: 385 575 385
Size: 190 4137
ApaLI G'TGCA_C
Cuts at: 264 3097 264
Size: 2833 1494
ApoI r'AATT_y
Cuts at: 1329 3680 3936 1329
Size: 2351 256 1720
Fragments arranged by size:
2351 1720 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4327
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4327
AvaI C'yCGr_G
Cuts at: 1141 1345 1141
Size: 204 4123
BamHI G'GATC_C
Cuts at: 1350 1350
Size: 4327
BanI G'GyrC_C
Cuts at: 531 628 822 910 1073 1084 1341 1755 2003
Size: 97 194 88 163 11 257 414 248
Cuts at: 2003 2038 531
Size: 35 2820
Fragments arranged by size:
2820 414 257 248 194 163 97 88
35 11
BanII G_rGCy'C
Cuts at: 385 393 575 923 1179 1339 2369 385
Size: 8 182 348 256 160 1030 2343
Fragments arranged by size:
2343 1030 348 256 182 160 8
BbeI G_GCGC'C
Cuts at: 535 826 914 1759 2007 535
Size: 291 88 845 248 2855
Fragments arranged by size:
2855 845 291 248 88
BciVI GTATCCnnnnn_n'
Cuts at: 2223 2986 4327 2223
Size: 763 1341 2223
Fragments arranged by size:
2223 1341 763
BclI T'GATC_A
Cuts at: 1845 1845
Size: 4327
BglI GCCn_nnn'nGGC
Cuts at: 494 831 494
Size: 337 3990
BglII A'GATC_T
Cuts at: 1840 3879 1840
Size: 2039 2288
BlpI GC'TnA_GC
Cuts at: 1437 1437
Size: 4327
Bme1580I G_kGCm'C
Cuts at: 268 385 575 1950 2043 3101 268
Size: 117 190 1375 93 1058 1494
Fragments arranged by size:
1494 1375 1058 190 117 93
BmgBI CAC'GTC
Cuts at: 710 710
Size: 4327
BmrI ACTGGGnnnn_n'
Cuts at: 673 1044 1537 1618 1953 4099 673
Size: 371 493 81 335 2146 901
Fragments arranged by size:
2146 901 493 371 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 393 407 253
Size: 140 14 4173
Fragments arranged by size:
4173 140 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1501 2380 2874 3172 1501
Size: 879 494 298 2656
Fragments arranged by size:
2656 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4031 4031
Size: 4327
BsaAI yAC'GTr
Cuts at: 513 645 1317 1678 513
Size: 132 672 361 3162
Fragments arranged by size:
3162 672 361 132
BsaBI GATnn'nnATC
Cuts at: 6 1839 3878 6
Size: 1833 2039 455
Fragments arranged by size:
2039 1833 455
BsaHI Gr'CG_yC
Cuts at: 446 532 608 823 911 1756 2004 2708 4204
Size: 86 76 215 88 845 248 704 1496
Cuts at: 4204 446
Size: 569
Fragments arranged by size:
1496 845 704 569 248 215 88 86
76
BsaWI w'CCGG_w
Cuts at: 290 1001 1514 2035 2715 2989 3136 3547 290
Size: 711 513 521 680 274 147 411 1070
Fragments arranged by size:
1070 711 680 521 513 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 667 697 820 850 667
Size: 30 123 30 4144
Fragments arranged by size:
4144 123 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 462 889 1027 462
Size: 427 138 3762
Fragments arranged by size:
3762 427 138
BseYI C'CCAG_C
Cuts at: 839 854 1109 1123 1259 1750 2508 3087 3583
Size: 15 255 14 136 491 758 579 496
Cuts at: 3583 839
Size: 1583
Fragments arranged by size:
1583 758 579 496 491 255 136 15
14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 406 271
Size: 135 4192
BsiEI CG_ry'CG
Cuts at: 104 260 317 1002 1867 1913 3123 104
Size: 156 57 685 865 46 1210 1308
Fragments arranged by size:
1308 1210 865 685 156 57 46
BsiHKAI G_wGCw'C
Cuts at: 268 1239 1339 2117 2307 3101 268
Size: 971 100 778 190 794 1494
Fragments arranged by size:
1494 971 794 778 190 100
Bsp1286I G_dGCh'C
Cuts at: 268 385 393 575 923 1179 1239 1339 1950
Size: 117 8 182 348 256 60 100 611
Cuts at: 1950 2043 2117 2307 2369 3101 268
Size: 93 74 190 62 732 1494
Fragments arranged by size:
1494 732 611 348 256 190 182 117
100 93 74 62 60 8
BspEI T'CCGG_A
Cuts at: 3547 3547
Size: 4327
BspHI T'CATG_A
Cuts at: 3503 3503
Size: 4327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1375 1589 1891 2272 1375
Size: 214 302 381 3430
Fragments arranged by size:
3430 381 302 214
BsrBI CCG'CTC
Cuts at: 50 404 2617 2671 4157 4322 50
Size: 354 2213 54 1486 165 55
Fragments arranged by size:
2213 1486 354 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2237 2237
Size: 4327
BsrFI r'CCGG_y
Cuts at: 830 1001 2323 2504 2715 830
Size: 171 1322 181 211 2442
Fragments arranged by size:
2442 1322 211 181 171
BssSI C'ACGA_G
Cuts at: 2596 2956 2596
Size: 360 3967
BstAPI GCAn_nnn'nTGC
Cuts at: 285 345 1702 285
Size: 60 1357 2910
Fragments arranged by size:
2910 1357 60
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4327
BstXI CCAn_nnnn'nTGG
Cuts at: 132 132
Size: 4327
BstYI r'GATC_y
Cuts at: 1 281 1350 1817 1840 2175 2421 3435 3521
Size: 280 1069 467 23 335 246 1014 86
Cuts at: 3521 3533 3879 1
Size: 12 346 449
Fragments arranged by size:
1069 1014 467 449 346 335 280 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 303 303
Size: 4327
BtgI C'CryG_G
Cuts at: 131 818 914 923 951 131
Size: 687 96 9 28 3507
Fragments arranged by size:
3507 687 96 28 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 750 2275 2456 2619 750
Size: 1525 181 163 2458
Fragments arranged by size:
2458 1525 181 163
BtsI GCAGTG_nn'
Cuts at: 1568 1568
Size: 4327
DraI TTT'AAA
Cuts at: 1324 1324
Size: 4327
DrdI GACnn_nn'nnGTC
Cuts at: 1251 2031 2891 1251
Size: 780 860 2687
Fragments arranged by size:
2687 860 780
EaeI y'GGCC_r
Cuts at: 151 257 308 704 948 1910 2084 2475 2502
Size: 106 51 396 244 962 174 391 27
Cuts at: 2502 3849 151
Size: 1347 629
Fragments arranged by size:
1347 962 629 396 391 244 174 106
51 27
EagI C'GGCC_G
Cuts at: 257 1910 257
Size: 1653 2674
EarI CTCTTCn'nnn_
Cuts at: 855 2348 2558 855
Size: 1493 210 2624
Fragments arranged by size:
2624 1493 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2533 2845 2991 4137 2533
Size: 312 146 1146 2723
Fragments arranged by size:
2723 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1337 1337
Size: 4327
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 393 407 2150 2582 3331 3599 253
Size: 140 14 1743 432 749 268 981
Fragments arranged by size:
1743 981 749 432 268 140 14
EcoNI CCTnn'n_nnAGG
Cuts at: 481 868 481
Size: 387 3940
EcoO109I rG'GnC_Cy
Cuts at: 382 417 592 1464 382
Size: 35 175 872 3245
Fragments arranged by size:
3245 872 175 35
EcoRI G'AATT_C
Cuts at: 1329 1329
Size: 4327
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 4327
FspI TGC'GCA
Cuts at: 2106 2106
Size: 4327
HaeII r_GCGC'y
Cuts at: 535 826 914 1252 1759 2007 3031 4010 535
Size: 291 88 338 507 248 1024 979 852
Fragments arranged by size:
1024 979 852 507 338 291 248 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2167 2197 2199 2229 2167
Size: 30 2 30 4265
Fragments arranged by size:
4265 30 30 2
HincII GTy'rAC
Cuts at: 1364 1364
Size: 4327
KasI G'GCGC_C
Cuts at: 531 822 910 1755 2003 531
Size: 291 88 845 248 2855
Fragments arranged by size:
2855 845 291 248 88
KpnI G_GTAC'C
Cuts at: 1077 1345 1077
Size: 268 4059
MluI A'CGCG_T
Cuts at: 3760 3760
Size: 4327
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 350 698 2998 3182 350
Size: 348 2300 184 1495
Fragments arranged by size:
2300 1495 348 184
MscI TGG'CCA
Cuts at: 310 2086 310
Size: 1776 2551
MslI CAynn'nnrTG
Cuts at: 130 556 664 769 1031 2314 2441 130
Size: 426 108 105 262 1283 127 2016
Fragments arranged by size:
2016 1283 426 262 127 108 105
MspA1I CmG'CkG
Cuts at: 813 916 1269 1436 1750 2110 3125 3370 813
Size: 103 353 167 314 360 1015 245 1770
Fragments arranged by size:
1770 1015 360 353 314 245 167 103
NaeI GCC'GGC
Cuts at: 2506 2506
Size: 4327
NarI GG'CG_CC
Cuts at: 532 823 911 1756 2004 532
Size: 291 88 845 248 2855
Fragments arranged by size:
2855 845 291 248 88
NcoI C'CATG_G
Cuts at: 818 923 951 818
Size: 105 28 4194
Fragments arranged by size:
4194 105 28
NgoMIV G'CCGG_C
Cuts at: 2504 2504
Size: 4327
NruI TCG'CGA
Cuts at: 2688 2688
Size: 4327
NspI r_CATG'y
Cuts at: 339 669 1378 1878 2787 339
Size: 330 709 500 909 1879
Fragments arranged by size:
1879 909 709 500 330
PasI CC'CwG_GG
Cuts at: 1170 1170
Size: 4327
PciI A'CATG_T
Cuts at: 2783 2783
Size: 4327
PfoI T'CCnGG_A
Cuts at: 230 230
Size: 4327
PmeI GTTT'AAAC
Cuts at: 1324 1324
Size: 4327
PmlI CAC'GTG
Cuts at: 513 513
Size: 4327
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3466 3498 3466
Size: 32 4295
PpuMI rG'GwC_Cy
Cuts at: 592 592
Size: 4327
PspOMI G'GGCC_C
Cuts at: 381 571 381
Size: 190 4137
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 286 318 286
Size: 32 4295
PstI C_TGCA'G
Cuts at: 485 1372 2057 485
Size: 887 685 2755
Fragments arranged by size:
2755 887 685
PvuI CG_AT'CG
Cuts at: 104 317 104
Size: 213 4114
PvuII CAG'CTG
Cuts at: 1750 2110 1750
Size: 360 3967
RsrII CG'GwC_CG
Cuts at: 2520 2520
Size: 4327
SacI G_AGCT'C
Cuts at: 1339 1339
Size: 4327
SacII CC_GC'GG
Cuts at: 917 917
Size: 4327
SalI G'TCGA_C
Cuts at: 1362 1362
Size: 4327
SanDI GG'GwC_CC
Cuts at: 592 592
Size: 4327
SapI GCTCTTCn'nnn_
Cuts at: 855 2348 2558 855
Size: 1493 210 2624
Fragments arranged by size:
2624 1493 210
SbfI CC_TGCA'GG
Cuts at: 485 1372 485
Size: 887 3440
ScaI AGT'ACT
Cuts at: 880 937 3844 880
Size: 57 2907 1363
Fragments arranged by size:
2907 1363 57
SexAI A'CCwGG_T
Cuts at: 296 296
Size: 4327
SfcI C'TryA_G
Cuts at: 33 481 579 717 1368 2053 3048 3239 33
Size: 448 98 138 651 685 995 191 1121
Fragments arranged by size:
1121 995 685 651 448 191 138 98
SfoI GGC'GCC
Cuts at: 533 824 912 1757 2005 533
Size: 291 88 845 248 2855
Fragments arranged by size:
2855 845 291 248 88
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4327
SmaI CCC'GGG
Cuts at: 1143 1347 1143
Size: 204 4123
SmlI C'TyrA_G
Cuts at: 1480 2395 2889 3151 1480
Size: 915 494 262 2656
Fragments arranged by size:
2656 915 494 262
SnaBI TAC'GTA
Cuts at: 1317 1317
Size: 4327
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4327
SphI G_CATG'C
Cuts at: 1378 1878 1378
Size: 500 3827
StyI C'CwwG_G
Cuts at: 818 923 951 1209 1459 1813 818
Size: 105 28 258 250 354 3332
Fragments arranged by size:
3332 354 258 250 105 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1908 2041 2500 2711 1908
Size: 133 459 211 3524
Fragments arranged by size:
3524 459 211 133
TatI w'GTAC_w
Cuts at: 878 893 935 1052 3842 878
Size: 15 42 117 2790 1363
Fragments arranged by size:
2790 1363 117 42 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3844 3876 3844
Size: 32 4295
Tth111I GACn'n_nGTC
Cuts at: 2122 2122
Size: 4327
XbaI T'CTAG_A
Cuts at: 1356 1356
Size: 4327
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 771 1820 771
Size: 1049 3278
XmaI C'CCGG_G
Cuts at: 1141 1345 1141
Size: 204 4123
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BssHII BstEII Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NsiI PacI PflMI PshAI PsiI PspXI
SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI
ZraI