Restriction map for
pF1KSDA0696
(Circular) (Six-base) MAPSORT of: pF1KSDA0696.seq Check: 5224 from: 1 to: 4765
pF1KSDA0696 4765 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 854 1232 1801 854
Size: 378 569 3818
Fragments arranged by size:
3818 569 378
Acc65I G'GTAC_C
Cuts at: 1779 1779
Size: 4765
AclI AA'CG_TT
Cuts at: 4605 4605
Size: 4765
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 1001 1092 2588 3020 3769 4037 478
Size: 523 91 1496 432 749 268 1206
Fragments arranged by size:
1496 1206 749 523 432 268 91
AflIII A'CryG_T
Cuts at: 1605 2115 3221 4198 1605
Size: 510 1106 977 2172
Fragments arranged by size:
2172 1106 977 510
AgeI A'CCGG_T
Cuts at: 3153 3153
Size: 4765
AhdI GACnn_n'nnGTC
Cuts at: 372 372
Size: 4765
AleI CACnn'nnGTG
Cuts at: 420 1588 420
Size: 1168 3597
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 252 252
Size: 4765
AlwNI CAG_nnn'CTG
Cuts at: 696 970 2068 3637 696
Size: 274 1098 1569 1824
Fragments arranged by size:
1824 1569 1098 274
ApaLI G'TGCA_C
Cuts at: 3535 3535
Size: 4765
ApoI r'AATT_y
Cuts at: 394 729 1306 1767 4118 4374 394
Size: 335 577 461 2351 256 785
Fragments arranged by size:
2351 785 577 461 335 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4765
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4765
AvaI C'yCGr_G
Cuts at: 1553 1783 1553
Size: 230 4535
BamHI G'GATC_C
Cuts at: 1788 1788
Size: 4765
BanI G'GyrC_C
Cuts at: 1423 1779 2193 2441 2476 1423
Size: 356 414 248 35 3712
Fragments arranged by size:
3712 414 356 248 35
BanII G_rGCy'C
Cuts at: 134 167 1560 1777 2807 134
Size: 33 1393 217 1030 2092
Fragments arranged by size:
2092 1393 1030 217 33
BbeI G_GCGC'C
Cuts at: 2197 2445 2197
Size: 248 4517
BciVI GTATCCnnnnn_n'
Cuts at: 2661 3424 4765 2661
Size: 763 1341 2661
Fragments arranged by size:
2661 1341 763
BclI T'GATC_A
Cuts at: 2283 2283
Size: 4765
BfrBI ATG'CAT
Cuts at: 1469 1469
Size: 4765
BglII A'GATC_T
Cuts at: 2278 4317 2278
Size: 2039 2726
BlpI GC'TnA_GC
Cuts at: 1875 1875
Size: 4765
Bme1580I G_kGCm'C
Cuts at: 1331 1704 2388 2481 3539 1331
Size: 373 684 93 1058 2557
Fragments arranged by size:
2557 1058 684 373 93
BmgBI CAC'GTC
Cuts at: 1606 1606
Size: 4765
BmrI ACTGGGnnnn_n'
Cuts at: 248 1691 1975 2056 2391 4537 248
Size: 1443 284 81 335 2146 476
Fragments arranged by size:
2146 1443 476 335 284 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 153 3130 153
Size: 2977 1788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 288 1308 1336 1603 1710 253
Size: 35 1020 28 267 107 3308
Fragments arranged by size:
3308 1020 267 107 35 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1939 2818 3312 3610 1939
Size: 879 494 298 3094
Fragments arranged by size:
3094 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 557 1705 4469 557
Size: 1148 2764 853
Fragments arranged by size:
2764 1148 853
BsaAI yAC'GTr
Cuts at: 1755 2116 1755
Size: 361 4404
BsaBI GATnn'nnATC
Cuts at: 6 2277 4316 6
Size: 2271 2039 455
Fragments arranged by size:
2271 2039 455
BsaHI Gr'CG_yC
Cuts at: 2194 2442 3146 4642 2194
Size: 248 704 1496 2317
Fragments arranged by size:
2317 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1472 1952 2473 3153 3427 3574 3985 1472
Size: 480 521 680 274 147 411 2252
Fragments arranged by size:
2252 680 521 480 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1704 1734 1704
Size: 30 4735
BseYI C'CCAG_C
Cuts at: 1560 2188 2946 3525 4021 1560
Size: 628 758 579 496 2304
Fragments arranged by size:
2304 758 628 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 581
Size: 4765
BsiEI CG_ry'CG
Cuts at: 104 1178 2305 2351 3561 104
Size: 1074 1127 46 1210 1308
Fragments arranged by size:
1308 1210 1127 1074 46
BsiHKAI G_wGCw'C
Cuts at: 167 1294 1300 1777 2555 2745 3539 167
Size: 1127 6 477 778 190 794 1393
Fragments arranged by size:
1393 1127 794 778 477 190 6
BsmI GAATG_Cn'
Cuts at: 614 614
Size: 4765
BsmBI CGTCTCn'nnnn_
Cuts at: 306 306
Size: 4765
Bsp1286I G_dGCh'C
Cuts at: 134 167 1294 1300 1331 1560 1704 1777 2388
Size: 33 1127 6 31 229 144 73 611
Cuts at: 2388 2481 2555 2745 2807 3539 134
Size: 93 74 190 62 732 1360
Fragments arranged by size:
1360 1127 732 611 229 190 144 93
74 73 62 33 31 6
BspEI T'CCGG_A
Cuts at: 3985 3985
Size: 4765
BspHI T'CATG_A
Cuts at: 3941 3941
Size: 4765
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1813 2027 2329 2710 1813
Size: 214 302 381 3868
Fragments arranged by size:
3868 381 302 214
BsrBI CCG'CTC
Cuts at: 50 833 1145 3055 3109 4595 4760 50
Size: 783 312 1910 54 1486 165 55
Fragments arranged by size:
1910 1486 783 312 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1119 1148 1340 2675 1119
Size: 29 192 1335 3209
Fragments arranged by size:
3209 1335 192 29
BsrFI r'CCGG_y
Cuts at: 2761 2942 3153 2761
Size: 181 211 4373
Fragments arranged by size:
4373 211 181
BssSI C'ACGA_G
Cuts at: 1293 3034 3394 1293
Size: 1741 360 2664
Fragments arranged by size:
2664 1741 360
BstAPI GCAn_nnn'nTGC
Cuts at: 249 2140 249
Size: 1891 2874
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4765
BstEII G'GTnAC_C
Cuts at: 1125 1125
Size: 4765
BstYI r'GATC_y
Cuts at: 1 1788 2255 2278 2613 2859 3873 3959 3971
Size: 1787 467 23 335 246 1014 86 12
Cuts at: 3971 4317 1
Size: 346 449
Fragments arranged by size:
1787 1014 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 4765
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2713 2894 3057 2713
Size: 181 163 4421
Fragments arranged by size:
4421 181 163
BtsI GCAGTG_nn'
Cuts at: 2006 2006
Size: 4765
DraI TTT'AAA
Cuts at: 702 1762 702
Size: 1060 3705
DraIII CAC_nnn'GTG
Cuts at: 1574 1574
Size: 4765
DrdI GACnn_nn'nnGTC
Cuts at: 2469 3329 2469
Size: 860 3905
EaeI y'GGCC_r
Cuts at: 1206 2348 2522 2913 2940 4287 1206
Size: 1142 174 391 27 1347 1684
Fragments arranged by size:
1684 1347 1142 391 174 27
EagI C'GGCC_G
Cuts at: 2348 2348
Size: 4765
EarI CTCTTCn'nnn_
Cuts at: 672 1020 2786 2996 672
Size: 348 1766 210 2441
Fragments arranged by size:
2441 1766 348 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2971 3283 3429 4575 2971
Size: 312 146 1146 3161
Fragments arranged by size:
3161 1146 312 146
EcoICRI GAG'CTC
Cuts at: 165 1775 165
Size: 1610 3155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 288 478 1001 1092 1308 1336 1603 1710
Size: 35 190 523 91 216 28 267 107
Cuts at: 1710 2588 3020 3769 4037 253
Size: 878 432 749 268 981
Fragments arranged by size:
981 878 749 523 432 268 267 216
190 107 91 35 28
EcoO109I rG'GnC_Cy
Cuts at: 1502 1902 1502
Size: 400 4365
EcoRI G'AATT_C
Cuts at: 1767 1767
Size: 4765
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 628 1561 2782 628
Size: 933 1221 2611
Fragments arranged by size:
2611 1221 933
FspI TGC'GCA
Cuts at: 228 1582 2544 228
Size: 1354 962 2449
Fragments arranged by size:
2449 1354 962
FspAI rTGC'GCAy
Cuts at: 228 228
Size: 4765
HaeII r_GCGC'y
Cuts at: 2197 2445 3469 4448 2197
Size: 248 1024 979 2514
Fragments arranged by size:
2514 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 358 390 535 567 1704 1736 2605 2635 2637
Size: 32 145 32 1137 32 869 30 2
Cuts at: 2637 2667 358
Size: 30 2456
Fragments arranged by size:
2456 1137 869 145 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 953 1802 953
Size: 849 3916
HpaI GTT'AAC
Cuts at: 953 953
Size: 4765
KasI G'GCGC_C
Cuts at: 2193 2441 2193
Size: 248 4517
KpnI G_GTAC'C
Cuts at: 1783 1783
Size: 4765
MluI A'CGCG_T
Cuts at: 4198 4198
Size: 4765
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3436 3620 3436
Size: 184 4581
MscI TGG'CCA
Cuts at: 1208 2524 1208
Size: 1316 3449
MslI CAynn'nnrTG
Cuts at: 420 1082 1153 1588 2752 2879 420
Size: 662 71 435 1164 127 2306
Fragments arranged by size:
2306 1164 662 435 127 71
MspA1I CmG'CkG
Cuts at: 474 1874 2188 2548 3563 3808 474
Size: 1400 314 360 1015 245 1431
Fragments arranged by size:
1431 1400 1015 360 314 245
NaeI GCC'GGC
Cuts at: 2944 2944
Size: 4765
NarI GG'CG_CC
Cuts at: 2194 2442 2194
Size: 248 4517
NcoI C'CATG_G
Cuts at: 125 125
Size: 4765
NgoMIV G'CCGG_C
Cuts at: 2942 2942
Size: 4765
NruI TCG'CGA
Cuts at: 3126 3126
Size: 4765
NsiI A_TGCA'T
Cuts at: 1471 1471
Size: 4765
NspI r_CATG'y
Cuts at: 225 252 1816 2316 3225 225
Size: 27 1564 500 909 1765
Fragments arranged by size:
1765 1564 909 500 27
PciI A'CATG_T
Cuts at: 3221 3221
Size: 4765
PfoI T'CCnGG_A
Cuts at: 764 764
Size: 4765
PmeI GTTT'AAAC
Cuts at: 1762 1762
Size: 4765
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3904 3936 3904
Size: 32 4733
PspXI vC'TCGA_Gb
Cuts at: 1553 1553
Size: 4765
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 623 655 623
Size: 32 4733
PstI C_TGCA'G
Cuts at: 245 986 1810 2495 245
Size: 741 824 685 2515
Fragments arranged by size:
2515 824 741 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4765
PvuII CAG'CTG
Cuts at: 2188 2548 2188
Size: 360 4405
RsrII CG'GwC_CG
Cuts at: 2958 2958
Size: 4765
SacI G_AGCT'C
Cuts at: 167 1777 167
Size: 1610 3155
SalI G'TCGA_C
Cuts at: 1800 1800
Size: 4765
SapI GCTCTTCn'nnn_
Cuts at: 1020 2786 2996 1020
Size: 1766 210 2789
Fragments arranged by size:
2789 1766 210
SbfI CC_TGCA'GG
Cuts at: 1810 1810
Size: 4765
ScaI AGT'ACT
Cuts at: 4282 4282
Size: 4765
SexAI A'CCwGG_T
Cuts at: 209 209
Size: 4765
SfcI C'TryA_G
Cuts at: 33 241 779 982 1806 2491 3486 3677 33
Size: 208 538 203 824 685 995 191 1121
Fragments arranged by size:
1121 995 824 685 538 208 203 191
SfoI GGC'GCC
Cuts at: 2195 2443 2195
Size: 248 4517
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4765
SmaI CCC'GGG
Cuts at: 1785 1785
Size: 4765
SmlI C'TyrA_G
Cuts at: 1553 1918 2833 3327 3589 1553
Size: 365 915 494 262 2729
Fragments arranged by size:
2729 915 494 365 262
SnaBI TAC'GTA
Cuts at: 1755 1755
Size: 4765
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4765
SphI G_CATG'C
Cuts at: 252 1816 2316 252
Size: 1564 500 2701
Fragments arranged by size:
2701 1564 500
SspI AAT'ATT
Cuts at: 361 361
Size: 4765
StyI C'CwwG_G
Cuts at: 125 181 1136 1897 2251 125
Size: 56 955 761 354 2639
Fragments arranged by size:
2639 955 761 354 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1192 2346 2479 2938 3149 1192
Size: 1154 133 459 211 2808
Fragments arranged by size:
2808 1154 459 211 133
TatI w'GTAC_w
Cuts at: 1247 1355 4280 1247
Size: 108 2925 1732
Fragments arranged by size:
2925 1732 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1109 1141 1482 1514 4282 4314 1109
Size: 32 341 32 2768 32 1560
Fragments arranged by size:
2768 1560 341 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2560 2560
Size: 4765
XbaI T'CTAG_A
Cuts at: 1747 1794 1747
Size: 47 4718
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 188 277 2258 188
Size: 89 1981 2695
Fragments arranged by size:
2695 1981 89
XhoI C'TCGA_G
Cuts at: 1553 1553
Size: 4765
XmaI C'CCGG_G
Cuts at: 1783 1783
Size: 4765
XmnI GAAnn'nnTTC
Cuts at: 527 1310 527
Size: 783 3982
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI
NspI PciI PfoI PmeI PpiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BglI BmtI Bpu10I BseRI
BsiWI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI HindIII MfeI NdeI NheI NotI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI
SacII SanDI SfiI SgrAI SrfI StuI SwaI ZraI