Restriction map for
pF1KSDA0712
(Circular) (Six-base) MAPSORT of: pF1KSDA0712.seq Check: 4931 from: 1 to: 8353
pF1KSDA0712 8353 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3275 3275
Size: 8353
AccI GT'mk_AC
Cuts at: 2284 2513 5389 2284
Size: 229 2876 5248
Fragments arranged by size:
5248 2876 229
Acc65I G'GTAC_C
Cuts at: 5367 5367
Size: 8353
AclI AA'CG_TT
Cuts at: 8193 8193
Size: 8353
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 681 955 1115 1636 1718 2015 2045 2339
Size: 506 274 160 521 82 297 30 294
Cuts at: 2339 2564 2648 2950 3973 6176 6608 7357 7625
Size: 225 84 302 1023 2203 432 749 268
Cuts at: 7625 175
Size: 903
Fragments arranged by size:
2203 1023 903 749 521 506 432 302
297 294 274 268 225 160 84 82
30
AflIII A'CryG_T
Cuts at: 5703 6809 7786 5703
Size: 1106 977 6270
Fragments arranged by size:
6270 1106 977
AgeI A'CCGG_T
Cuts at: 6741 6741
Size: 8353
AhdI GACnn_n'nnGTC
Cuts at: 3150 3150
Size: 8353
AleI CACnn'nnGTG
Cuts at: 3427 3688 3427
Size: 261 8092
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 817 4964 817
Size: 4147 4206
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2973 3005 2973
Size: 32 8321
AlwNI CAG_nnn'CTG
Cuts at: 153 2007 2458 4471 4973 5656 7225 153
Size: 1854 451 2013 502 683 1569 1281
Fragments arranged by size:
2013 1854 1569 1281 683 502 451
ApaI G_GGCC'C
Cuts at: 4077 4763 4077
Size: 686 7667
ApaLI G'TGCA_C
Cuts at: 7123 7123
Size: 8353
ApoI r'AATT_y
Cuts at: 216 340 472 979 2764 5355 7706 7962 216
Size: 124 132 507 1785 2591 2351 256 607
Fragments arranged by size:
2591 2351 1785 607 507 256 132 124
AseI AT'TA_AT
Cuts at: 20 20
Size: 8353
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8353
AvaI C'yCGr_G
Cuts at: 3131 3248 4211 4540 4573 5371 3131
Size: 117 963 329 33 798 6113
Fragments arranged by size:
6113 963 798 329 117 33
AvrII C'CTAG_G
Cuts at: 4159 4159
Size: 8353
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3913 3946 3913
Size: 33 8320
BamHI G'GATC_C
Cuts at: 2463 5376 2463
Size: 2913 5440
BanI G'GyrC_C
Cuts at: 3244 3640 5367 5781 6029 6064 3244
Size: 396 1727 414 248 35 5533
Fragments arranged by size:
5533 1727 414 396 248 35
BanII G_rGCy'C
Cuts at: 657 1432 1693 3766 4077 4596 4763 5365 6395
Size: 775 261 2073 311 519 167 602 1030
Cuts at: 6395 657
Size: 2615
Fragments arranged by size:
2615 2073 1030 775 602 519 311 261
167
BbeI G_GCGC'C
Cuts at: 5785 6033 5785
Size: 248 8105
BbsI GAAGACnn'nnnn_
Cuts at: 1263 2636 3664 3746 3800 3842 4690 1263
Size: 1373 1028 82 54 42 848 4926
Fragments arranged by size:
4926 1373 1028 848 82 54 42
BbvCI CC'TCA_GC
Cuts at: 1412 5177 1412
Size: 3765 4588
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3723 3757 3723
Size: 34 8319
BciVI GTATCCnnnnn_n'
Cuts at: 858 1540 6249 7012 8353 858
Size: 682 4709 763 1341 858
Fragments arranged by size:
4709 1341 858 763 682
BclI T'GATC_A
Cuts at: 2610 5871 2610
Size: 3261 5092
BfrBI ATG'CAT
Cuts at: 627 1879 3439 627
Size: 1252 1560 5541
Fragments arranged by size:
5541 1560 1252
BglII A'GATC_T
Cuts at: 1264 3016 5866 7905 1264
Size: 1752 2850 2039 1712
Fragments arranged by size:
2850 2039 1752 1712
BlpI GC'TnA_GC
Cuts at: 5463 5463
Size: 8353
Bme1580I G_kGCm'C
Cuts at: 3249 4077 4716 4763 5228 5976 6069 7127 3249
Size: 828 639 47 465 748 93 1058 4475
Fragments arranged by size:
4475 1058 828 748 639 465 93 47
BmgBI CAC'GTC
Cuts at: 357 4207 357
Size: 3850 4503
BmrI ACTGGGnnnn_n'
Cuts at: 2468 5075 5136 5563 5644 5979 8125 2468
Size: 2607 61 427 81 335 2146 2696
Fragments arranged by size:
2696 2607 2146 427 335 81 61
BmtI G_CTAG'C
Cuts at: 2215 2215
Size: 8353
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 4376 4980 6718 3617
Size: 759 604 1738 5252
Fragments arranged by size:
5252 1738 759 604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 1534 1598 2389 2864 3348 4868 4950 589
Size: 945 64 791 475 484 1520 82 3992
Fragments arranged by size:
3992 1520 945 791 484 475 82 64
Bpu10I CC'TnA_GC
Cuts at: 905 1412 3470 4703 5177 905
Size: 507 2058 1233 474 4081
Fragments arranged by size:
4081 2058 1233 507 474
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1446 1707 2049 4330 5527 6406 6900 7198 1446
Size: 261 342 2281 1197 879 494 298 2601
Fragments arranged by size:
2601 2281 1197 879 494 342 298 261
BsaI GGTCTCn'nnnn_
Cuts at: 2253 4107 8057 2253
Size: 1854 3950 2549
Fragments arranged by size:
3950 2549 1854
BsaAI yAC'GTr
Cuts at: 5343 5704 5343
Size: 361 7992
BsaBI GATnn'nnATC
Cuts at: 6 520 1463 5865 7904 6
Size: 514 943 4402 2039 455
Fragments arranged by size:
4402 2039 943 514 455
BsaHI Gr'CG_yC
Cuts at: 5782 6030 6734 8230 5782
Size: 248 704 1496 5905
Fragments arranged by size:
5905 1496 704 248
BsaWI w'CCGG_w
Cuts at: 3818 4343 5540 6061 6741 7015 7162 7573 3818
Size: 525 1197 521 680 274 147 411 4598
Fragments arranged by size:
4598 1197 680 525 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2862 2892 2862
Size: 30 8323
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1213 1375 1391 1637 3489 3756 4680 5046 5204
Size: 162 16 246 1852 267 924 366 158
Cuts at: 5204 1213
Size: 4362
Fragments arranged by size:
4362 1852 924 366 267 246 162 158
16
BseYI C'CCAG_C
Cuts at: 1308 1386 2278 2915 5776 6534 7113 7609 1308
Size: 78 892 637 2861 758 579 496 2052
Fragments arranged by size:
2861 2052 892 758 637 579 496 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 950 3247 3293 3392 3847 4438 237
Size: 713 2297 46 99 455 591 4152
Fragments arranged by size:
4152 2297 713 591 455 99 46
BsiEI CG_ry'CG
Cuts at: 104 2382 5893 5939 7149 104
Size: 2278 3511 46 1210 1308
Fragments arranged by size:
3511 2278 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 209 356 2902 3766 4362 4709 5365 6143 6333
Size: 147 2546 864 596 347 656 778 190
Cuts at: 6333 7127 209
Size: 794 1435
Fragments arranged by size:
2546 1435 864 794 778 656 596 347
190 147
BsmI GAATG_Cn'
Cuts at: 259 1881 259
Size: 1622 6731
Bsp1286I G_dGCh'C
Cuts at: 209 356 657 1432 1693 2902 3249 3766 4077
Size: 147 301 775 261 1209 347 517 311
Cuts at: 4077 4362 4596 4709 4716 4763 5228 5365 5976
Size: 285 234 113 7 47 465 137 611
Cuts at: 5976 6069 6143 6333 6395 7127 209
Size: 93 74 190 62 732 1435
Fragments arranged by size:
1435 1209 775 732 611 517 465 347
311 301 285 261 234 190 147 137
113 93 74 62 47 7
BspEI T'CCGG_A
Cuts at: 3818 4343 7573 3818
Size: 525 3230 4598
Fragments arranged by size:
4598 3230 525
BspHI T'CATG_A
Cuts at: 1344 7529 1344
Size: 6185 2168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 228 969 3036 3275 5401 5615 5917 6298 228
Size: 741 2067 239 2126 214 302 381 2283
Fragments arranged by size:
2283 2126 2067 741 381 302 239 214
BsrBI CCG'CTC
Cuts at: 50 3965 6643 6697 8183 8348 50
Size: 3915 2678 54 1486 165 55
Fragments arranged by size:
3915 2678 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3439 6263 3439
Size: 2824 5529
BsrFI r'CCGG_y
Cuts at: 6349 6530 6741 6349
Size: 181 211 7961
Fragments arranged by size:
7961 211 181
BsrGI T'GTAC_A
Cuts at: 4181 4181
Size: 8353
BssHII G'CGCG_C
Cuts at: 3270 3270
Size: 8353
BssSI C'ACGA_G
Cuts at: 6622 6982 6622
Size: 360 7993
BstAPI GCAn_nnn'nTGC
Cuts at: 1285 5728 1285
Size: 4443 3910
BstBI TT'CG_AA
Cuts at: 110 110
Size: 8353
BstXI CCAn_nnnn'nTGG
Cuts at: 3333 3429 3637 4780 4846 4920 3333
Size: 96 208 1143 66 74 6766
Fragments arranged by size:
6766 1143 208 96 74 66
BstYI r'GATC_y
Cuts at: 1 1264 1930 2463 3016 4305 4436 4467 5327
Size: 1263 666 533 553 1289 131 31 860
Cuts at: 5327 5376 5843 5866 6201 6447 7461 7547 7559
Size: 49 467 23 335 246 1014 86 12
Cuts at: 7559 7905 1
Size: 346 449
Fragments arranged by size:
1289 1263 1014 860 666 553 533 467
449 346 335 246 131 86 49 31
23 12
BstZ17I GTA'TAC
Cuts at: 2285 2285
Size: 8353
Bsu36I CC'TnA_GG
Cuts at: 1493 2132 4663 1493
Size: 639 2531 5183
Fragments arranged by size:
5183 2531 639
BtgI C'CryG_G
Cuts at: 1145 1828 3137 3197 3665 4611 1145
Size: 683 1309 60 468 946 4887
Fragments arranged by size:
4887 1309 946 683 468 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6301 6482 6645 6301
Size: 181 163 8009
Fragments arranged by size:
8009 181 163
BtsI GCAGTG_nn'
Cuts at: 2939 3313 3375 3410 5130 5594 2939
Size: 374 62 35 1720 464 5698
Fragments arranged by size:
5698 1720 464 374 62 35
DraI TTT'AAA
Cuts at: 5350 5350
Size: 8353
DraIII CAC_nnn'GTG
Cuts at: 3633 3633
Size: 8353
DrdI GACnn_nn'nnGTC
Cuts at: 5136 6057 6917 5136
Size: 921 860 6572
Fragments arranged by size:
6572 921 860
EaeI y'GGCC_r
Cuts at: 797 4008 4782 5936 6110 6501 6528 7875 797
Size: 3211 774 1154 174 391 27 1347 1275
Fragments arranged by size:
3211 1347 1275 1154 774 391 174 27
EagI C'GGCC_G
Cuts at: 5936 5936
Size: 8353
EarI CTCTTCn'nnn_
Cuts at: 599 875 1249 1655 2251 2831 6374 6584 599
Size: 276 374 406 596 580 3543 210 2368
Fragments arranged by size:
3543 2368 596 580 406 374 276 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2993 3833 6559 6871 7017 8163 2993
Size: 840 2726 312 146 1146 3183
Fragments arranged by size:
3183 2726 1146 840 312 146
EcoICRI GAG'CTC
Cuts at: 3764 5363 3764
Size: 1599 6754
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 589 681 955 1115 1534 1598 1636 1718
Size: 414 92 274 160 419 64 38 82
Cuts at: 1718 2015 2045 2339 2389 2564 2648 2864 2950
Size: 297 30 294 50 175 84 216 86
Cuts at: 2950 3348 3973 4868 4950 6176 6608 7357 7625
Size: 398 625 895 82 1226 432 749 268
Cuts at: 7625 175
Size: 903
Fragments arranged by size:
1226 903 895 749 625 432 419 414
398 297 294 274 268 216 175 160
92 86 84 82 82 64 50 38
30
EcoNI CCTnn'n_nnAGG
Cuts at: 2011 3507 3771 4935 2011
Size: 1496 264 1164 5429
Fragments arranged by size:
5429 1496 1164 264
EcoO109I rG'GnC_Cy
Cuts at: 958 2267 2556 2828 3252 4073 4074 4378 4760
Size: 1309 289 272 424 821 1 304 382
Cuts at: 4760 5076 5255 5282 5490 958
Size: 316 179 27 208 3821
Fragments arranged by size:
3821 1309 821 424 382 316 304 289
272 208 179 27 1
EcoRI G'AATT_C
Cuts at: 5355 5355
Size: 8353
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 441 6370 441
Size: 5929 2424
FspI TGC'GCA
Cuts at: 3290 6132 3290
Size: 2842 5511
HaeII r_GCGC'y
Cuts at: 2212 5118 5785 6033 7057 8036 2212
Size: 2906 667 248 1024 979 2529
Fragments arranged by size:
2906 2529 1024 979 667 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 263 295 1267 1299 1456 1488 1693 1725 2041
Size: 32 972 32 157 32 205 32 316
Cuts at: 2041 2073 2314 2346 2862 2894 3136 3168 3736
Size: 32 241 32 516 32 242 32 568
Cuts at: 3736 3768 4621 4653 4783 4815 5152 5184 6193
Size: 32 853 32 130 32 337 32 1009
Cuts at: 6193 6223 6225 6255 263
Size: 30 2 30 2361
Fragments arranged by size:
2361 1009 972 853 568 516 337 316
242 241 205 157 130 32 32 32
32 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1422 5390 1422
Size: 3968 4385
HindIII A'AGCT_T
Cuts at: 4675 4675
Size: 8353
KasI G'GCGC_C
Cuts at: 5781 6029 5781
Size: 248 8105
KpnI G_GTAC'C
Cuts at: 5371 5371
Size: 8353
MfeI C'AATT_G
Cuts at: 644 920 644
Size: 276 8077
MluI A'CGCG_T
Cuts at: 7786 7786
Size: 8353
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 163 1272 2501 7024 7208 163
Size: 1109 1229 4523 184 1308
Fragments arranged by size:
4523 1308 1229 1109 184
MscI TGG'CCA
Cuts at: 799 4784 6112 799
Size: 3985 1328 3040
Fragments arranged by size:
3985 3040 1328
MslI CAynn'nnrTG
Cuts at: 622 721 767 799 1349 1479 2305 3296 3328
Size: 99 46 32 550 130 826 991 32
Cuts at: 3328 3427 3688 6340 6467 622
Size: 99 261 2652 127 2508
Fragments arranged by size:
2652 2508 991 826 550 261 130 127
99 99 46 32 32
MspA1I CmG'CkG
Cuts at: 162 462 782 1113 1319 1411 2915 3208 3258
Size: 300 320 331 206 92 1504 293 50
Cuts at: 3258 5462 5776 6136 7151 7396 162
Size: 2204 314 360 1015 245 1119
Fragments arranged by size:
2204 1504 1119 1015 360 331 320 314
300 293 245 206 92 50
NaeI GCC'GGC
Cuts at: 6532 6532
Size: 8353
NarI GG'CG_CC
Cuts at: 5782 6030 5782
Size: 248 8105
NcoI C'CATG_G
Cuts at: 1145 3137 3197 3665 4611 1145
Size: 1992 60 468 946 4887
Fragments arranged by size:
4887 1992 946 468 60
NdeI CA'TA_TG
Cuts at: 1986 3687 1986
Size: 1701 6652
NgoMIV G'CCGG_C
Cuts at: 6530 6530
Size: 8353
NheI G'CTAG_C
Cuts at: 2211 2211
Size: 8353
NruI TCG'CGA
Cuts at: 6714 6714
Size: 8353
NsiI A_TGCA'T
Cuts at: 629 1881 3441 629
Size: 1252 1560 5541
Fragments arranged by size:
5541 1560 1252
NspI r_CATG'y
Cuts at: 631 3478 4266 4335 5404 5904 6813 631
Size: 2847 788 69 1069 500 909 2171
Fragments arranged by size:
2847 2171 1069 909 788 500 69
PasI CC'CwG_GG
Cuts at: 4773 4931 4773
Size: 158 8195
PciI A'CATG_T
Cuts at: 6809 6809
Size: 8353
PflMI CCAn_nnn'nTGG
Cuts at: 402 1441 2007 5067 402
Size: 1039 566 3060 3688
Fragments arranged by size:
3688 3060 1039 566
PfoI T'CCnGG_A
Cuts at: 428 1614 428
Size: 1186 7167
PmeI GTTT'AAAC
Cuts at: 5350 5350
Size: 8353
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 888 920 7492 7524 888
Size: 32 6572 32 1717
Fragments arranged by size:
6572 1717 32 32
PpuMI rG'GwC_Cy
Cuts at: 958 2267 2556 2828 3252 5076 5255 958
Size: 1309 289 272 424 1824 179 4056
Fragments arranged by size:
4056 1824 1309 424 289 272 179
PshAI GACnn'nnGTC
Cuts at: 588 588
Size: 8353
PsiI TTA'TAA
Cuts at: 3680 3680
Size: 8353
PspOMI G'GGCC_C
Cuts at: 4073 4759 4073
Size: 686 7667
PspXI vC'TCGA_Gb
Cuts at: 3131 3131
Size: 8353
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1044 1076 3092 3124 1044
Size: 32 2016 32 6273
Fragments arranged by size:
6273 2016 32 32
PstI C_TGCA'G
Cuts at: 1112 2349 3024 4696 5398 6083 1112
Size: 1237 675 1672 702 685 3382
Fragments arranged by size:
3382 1672 1237 702 685 675
PvuI CG_AT'CG
Cuts at: 104 2382 104
Size: 2278 6075
PvuII CAG'CTG
Cuts at: 462 1411 2915 3208 5776 6136 462
Size: 949 1504 293 2568 360 2679
Fragments arranged by size:
2679 2568 1504 949 360 293
RsrII CG'GwC_CG
Cuts at: 6546 6546
Size: 8353
SacI G_AGCT'C
Cuts at: 3766 5365 3766
Size: 1599 6754
SalI G'TCGA_C
Cuts at: 5388 5388
Size: 8353
SanDI GG'GwC_CC
Cuts at: 2556 2828 3252 5076 2556
Size: 272 424 1824 5833
Fragments arranged by size:
5833 1824 424 272
SapI GCTCTTCn'nnn_
Cuts at: 1249 6374 6584 1249
Size: 5125 210 3018
Fragments arranged by size:
5125 3018 210
SbfI CC_TGCA'GG
Cuts at: 5398 5398
Size: 8353
ScaI AGT'ACT
Cuts at: 7870 7870
Size: 8353
SexAI A'CCwGG_T
Cuts at: 2012 2012
Size: 8353
SfcI C'TryA_G
Cuts at: 33 558 1108 2084 2345 2700 3020 3203 4020
Size: 525 550 976 261 355 320 183 817
Cuts at: 4020 4560 4692 5264 5394 6079 7074 7265 33
Size: 540 132 572 130 685 995 191 1121
Fragments arranged by size:
1121 995 976 817 685 572 550 540
525 355 320 261 191 183 132 130
SfoI GGC'GCC
Cuts at: 5783 6031 5783
Size: 248 8105
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8353
SmaI CCC'GGG
Cuts at: 3250 4213 5373 3250
Size: 963 1160 6230
Fragments arranged by size:
6230 1160 963
SmlI C'TyrA_G
Cuts at: 1425 1686 2064 3131 4309 5506 6421 6915 7177
Size: 261 378 1067 1178 1197 915 494 262
Cuts at: 7177 1425
Size: 2601
Fragments arranged by size:
2601 1197 1178 1067 915 494 378 262
261
SnaBI TAC'GTA
Cuts at: 5343 5343
Size: 8353
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8353
SphI G_CATG'C
Cuts at: 631 3478 4266 4335 5404 5904 631
Size: 2847 788 69 1069 500 3080
Fragments arranged by size:
3080 2847 1069 788 500 69
StuI AGG'CCT
Cuts at: 1020 2365 3221 1020
Size: 1345 856 6152
Fragments arranged by size:
6152 1345 856
StyI C'CwwG_G
Cuts at: 1145 1553 2903 3137 3197 3665 4159 4526 4611
Size: 408 1350 234 60 468 494 367 85
Cuts at: 4611 5485 5839 1145
Size: 874 354 3659
Fragments arranged by size:
3659 1350 874 494 468 408 367 354
234 85 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1954 4420 5934 6067 6526 6737 1954
Size: 2466 1514 133 459 211 3570
Fragments arranged by size:
3570 2466 1514 459 211 133
TatI w'GTAC_w
Cuts at: 422 4181 7868 422
Size: 3759 3687 907
Fragments arranged by size:
3759 3687 907
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 557 589 1107 1139 3443 3475 7870 7902 557
Size: 32 518 32 2304 32 4395 32 1008
Fragments arranged by size:
4395 2304 1008 518 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6148 6148
Size: 8353
XbaI T'CTAG_A
Cuts at: 2355 5382 2355
Size: 3027 5326
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 840 1225 2919 3330 4792 5846 840
Size: 385 1694 411 1462 1054 3347
Fragments arranged by size:
3347 1694 1462 1054 411 385
XhoI C'TCGA_G
Cuts at: 3131 3131
Size: 8353
XmaI C'CCGG_G
Cuts at: 3248 4211 5371 3248
Size: 963 1160 6230
Fragments arranged by size:
6230 1160 963
XmnI GAAnn'nnTTC
Cuts at: 2577 3797 2577
Size: 1220 7133
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NruI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AscI BglI BsiWI BsmBI BstEII
ClaI CspCI EcoRV FseI FspAI HpaI NotI PacI
PmlI SacII SfiI SgrAI SrfI SspI SwaI ZraI