Restriction map for
pF1KSDA0723
(Circular) (Six-base) MAPSORT of: pF1KSDA0723.seq Check: 9322 from: 1 to: 5680
pF1KSDA0723 5680 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 1168 2716 1168
Size: 1548 4132
Acc65I G'GTAC_C
Cuts at: 2694 2694
Size: 5680
AclI AA'CG_TT
Cuts at: 5520 5520
Size: 5680
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 596 1586 2108 2228 3503 3935 4684 4952 596
Size: 990 522 120 1275 432 749 268 1324
Fragments arranged by size:
1324 1275 990 749 522 432 268 120
AflIII A'CryG_T
Cuts at: 1611 3030 4136 5113 1611
Size: 1419 1106 977 2178
Fragments arranged by size:
2178 1419 1106 977
AgeI A'CCGG_T
Cuts at: 4068 4068
Size: 5680
AlwNI CAG_nnn'CTG
Cuts at: 2359 2983 4552 2359
Size: 624 1569 3487
Fragments arranged by size:
3487 1569 624
ApaLI G'TGCA_C
Cuts at: 338 4450 338
Size: 4112 1568
ApoI r'AATT_y
Cuts at: 534 859 1187 2620 2682 5033 5289 534
Size: 325 328 1433 62 2351 256 925
Fragments arranged by size:
2351 1433 925 328 325 256 62
AseI AT'TA_AT
Cuts at: 20 1427 20
Size: 1407 4273
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5680
AvaI C'yCGr_G
Cuts at: 2698 2698
Size: 5680
AvrII C'CTAG_G
Cuts at: 646 646
Size: 5680
BamHI G'GATC_C
Cuts at: 2703 2703
Size: 5680
BanI G'GyrC_C
Cuts at: 2694 3108 3356 3391 2694
Size: 414 248 35 4983
Fragments arranged by size:
4983 414 248 35
BanII G_rGCy'C
Cuts at: 333 2692 3722 333
Size: 2359 1030 2291
Fragments arranged by size:
2359 2291 1030
BbeI G_GCGC'C
Cuts at: 3112 3360 3112
Size: 248 5432
BbsI GAAGACnn'nnnn_
Cuts at: 338 968 1981 338
Size: 630 1013 4037
Fragments arranged by size:
4037 1013 630
BciVI GTATCCnnnnn_n'
Cuts at: 3576 4339 5680 3576
Size: 763 1341 3576
Fragments arranged by size:
3576 1341 763
BclI T'GATC_A
Cuts at: 1590 2295 3198 1590
Size: 705 903 4072
Fragments arranged by size:
4072 903 705
BglII A'GATC_T
Cuts at: 613 913 1909 3193 5232 613
Size: 300 996 1284 2039 1061
Fragments arranged by size:
2039 1284 1061 996 300
BlpI GC'TnA_GC
Cuts at: 1676 2790 1676
Size: 1114 4566
Bme1580I G_kGCm'C
Cuts at: 342 3303 3396 4454 342
Size: 2961 93 1058 1568
Fragments arranged by size:
2961 1568 1058 93
BmgBI CAC'GTC
Cuts at: 889 1612 889
Size: 723 4957
BmrI ACTGGGnnnn_n'
Cuts at: 219 712 2890 2971 3306 5452 219
Size: 493 2178 81 335 2146 447
Fragments arranged by size:
2178 2146 493 447 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 424 956 4045 424
Size: 532 3089 2059
Fragments arranged by size:
3089 2059 532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1904 1904
Size: 5680
Bpu10I CC'TnA_GC
Cuts at: 1466 1466
Size: 5680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 612 734 1377 1651 2854 3733 4227 4525 612
Size: 122 643 274 1203 879 494 298 1767
Fragments arranged by size:
1767 1203 879 643 494 298 274 122
BsaI GGTCTCn'nnnn_
Cuts at: 832 2152 5384 832
Size: 1320 3232 1128
Fragments arranged by size:
3232 1320 1128
BsaAI yAC'GTr
Cuts at: 2670 3031 2670
Size: 361 5319
BsaBI GATnn'nnATC
Cuts at: 6 3192 5231 6
Size: 3186 2039 455
Fragments arranged by size:
3186 2039 455
BsaHI Gr'CG_yC
Cuts at: 3109 3357 4061 5557 3109
Size: 248 704 1496 3232
Fragments arranged by size:
3232 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2017 2867 3388 4068 4342 4489 4900 2017
Size: 850 521 680 274 147 411 2797
Fragments arranged by size:
2797 850 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 582 932 582
Size: 350 5330
BseYI C'CCAG_C
Cuts at: 3103 3861 4440 4936 3103
Size: 758 579 496 3847
Fragments arranged by size:
3847 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 257
Size: 5680
BsiEI CG_ry'CG
Cuts at: 104 3220 3266 4476 104
Size: 3116 46 1210 1308
Fragments arranged by size:
3116 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 333 342 1006 2692 3470 3660 4454 333
Size: 9 664 1686 778 190 794 1559
Fragments arranged by size:
1686 1559 794 778 664 190 9
BsmI GAATG_Cn'
Cuts at: 1638 1638
Size: 5680
BsmBI CGTCTCn'nnnn_
Cuts at: 2147 2147
Size: 5680
Bsp1286I G_dGCh'C
Cuts at: 333 342 1006 2692 3303 3396 3470 3660 3722
Size: 9 664 1686 611 93 74 190 62
Cuts at: 3722 4454 333
Size: 732 1559
Fragments arranged by size:
1686 1559 732 664 611 190 93 74
62 9
BspEI T'CCGG_A
Cuts at: 4900 4900
Size: 5680
BspHI T'CATG_A
Cuts at: 4856 4856
Size: 5680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1280 2728 2942 3244 3625 1280
Size: 1448 214 302 381 3335
Fragments arranged by size:
3335 1448 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3970 4024 5510 5675 50
Size: 3920 54 1486 165 55
Fragments arranged by size:
3920 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1766 1772 2583 3590 1766
Size: 6 811 1007 3856
Fragments arranged by size:
3856 1007 811 6
BsrFI r'CCGG_y
Cuts at: 3676 3857 4068 3676
Size: 181 211 5288
Fragments arranged by size:
5288 211 181
BssSI C'ACGA_G
Cuts at: 3949 4309 3949
Size: 360 5320
BstAPI GCAn_nnn'nTGC
Cuts at: 280 3055 280
Size: 2775 2905
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5680
BstYI r'GATC_y
Cuts at: 1 613 913 1909 2703 3170 3193 3528 3774
Size: 612 300 996 794 467 23 335 246
Cuts at: 3774 4788 4874 4886 5232 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 996 794 612 467 449 346 335
300 246 86 23 12
BtgI C'CryG_G
Cuts at: 429 735 969 429
Size: 306 234 5140
Fragments arranged by size:
5140 306 234
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3628 3809 3972 3628
Size: 181 163 5336
Fragments arranged by size:
5336 181 163
BtsI GCAGTG_nn'
Cuts at: 1481 2125 2921 1481
Size: 644 796 4240
Fragments arranged by size:
4240 796 644
DraI TTT'AAA
Cuts at: 2677 2677
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 3384 4244 3384
Size: 860 4820
EaeI y'GGCC_r
Cuts at: 461 3263 3437 3828 3855 5202 461
Size: 2802 174 391 27 1347 939
Fragments arranged by size:
2802 1347 939 391 174 27
EagI C'GGCC_G
Cuts at: 3263 3263
Size: 5680
EarI CTCTTCn'nnn_
Cuts at: 675 2003 3701 3911 675
Size: 1328 1698 210 2444
Fragments arranged by size:
2444 1698 1328 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3886 4198 4344 5490 3886
Size: 312 146 1146 4076
Fragments arranged by size:
4076 1146 312 146
EcoICRI GAG'CTC
Cuts at: 331 2690 331
Size: 2359 3321
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 596 1586 1904 2108 2228 3503 3935 4684 4952
Size: 990 318 204 120 1275 432 749 268
Cuts at: 4952 596
Size: 1324
Fragments arranged by size:
1324 1275 990 749 432 318 268 204
120
EcoNI CCTnn'n_nnAGG
Cuts at: 2519 2519
Size: 5680
EcoO109I rG'GnC_Cy
Cuts at: 583 1195 1279 2817 583
Size: 612 84 1538 3446
Fragments arranged by size:
3446 1538 612 84
EcoRI G'AATT_C
Cuts at: 1187 2682 1187
Size: 1495 4185
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1745 3697 1745
Size: 1952 3728
FspI TGC'GCA
Cuts at: 3459 3459
Size: 5680
HaeII r_GCGC'y
Cuts at: 941 3112 3360 4384 5363 941
Size: 2171 248 1024 979 1258
Fragments arranged by size:
2171 1258 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 492 524 598 630 1707 1739 1936 1968 3520
Size: 32 74 32 1077 32 197 32 1552
Cuts at: 3520 3550 3552 3582 492
Size: 30 2 30 2590
Fragments arranged by size:
2590 1552 1077 197 74 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1048 1773 1824 2717 1048
Size: 725 51 893 4011
Fragments arranged by size:
4011 893 725 51
HindIII A'AGCT_T
Cuts at: 1669 2263 1669
Size: 594 5086
KasI G'GCGC_C
Cuts at: 3108 3356 3108
Size: 248 5432
KpnI G_GTAC'C
Cuts at: 2698 2698
Size: 5680
MluI A'CGCG_T
Cuts at: 5113 5113
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1215 2297 4351 4535 1215
Size: 1082 2054 184 2360
Fragments arranged by size:
2360 2054 1082 184
MscI TGG'CCA
Cuts at: 463 3439 463
Size: 2976 2704
MslI CAynn'nnrTG
Cuts at: 179 1055 1708 3667 3794 179
Size: 876 653 1959 127 2065
Fragments arranged by size:
2065 1959 876 653 127
MspA1I CmG'CkG
Cuts at: 1377 2789 3103 3463 4478 4723 1377
Size: 1412 314 360 1015 245 2334
Fragments arranged by size:
2334 1412 1015 360 314 245
NaeI GCC'GGC
Cuts at: 3859 3859
Size: 5680
NarI GG'CG_CC
Cuts at: 3109 3357 3109
Size: 248 5432
NcoI C'CATG_G
Cuts at: 735 969 735
Size: 234 5446
NgoMIV G'CCGG_C
Cuts at: 3857 3857
Size: 5680
NruI TCG'CGA
Cuts at: 4041 4041
Size: 5680
NspI r_CATG'y
Cuts at: 1293 2731 3231 4140 1293
Size: 1438 500 909 2833
Fragments arranged by size:
2833 1438 909 500
PciI A'CATG_T
Cuts at: 4136 4136
Size: 5680
PflMI CCAn_nnn'nTGG
Cuts at: 1234 1234
Size: 5680
PmeI GTTT'AAAC
Cuts at: 2677 2677
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4819 4851 4819
Size: 32 5648
PpuMI rG'GwC_Cy
Cuts at: 1195 1279 1195
Size: 84 5596
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2526 2558 2526
Size: 32 5648
PstI C_TGCA'G
Cuts at: 2725 3410 2725
Size: 685 4995
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5680
PvuII CAG'CTG
Cuts at: 1377 3103 3463 1377
Size: 1726 360 3594
Fragments arranged by size:
3594 1726 360
RsrII CG'GwC_CG
Cuts at: 3873 3873
Size: 5680
SacI G_AGCT'C
Cuts at: 333 2692 333
Size: 2359 3321
SalI G'TCGA_C
Cuts at: 2715 2715
Size: 5680
SapI GCTCTTCn'nnn_
Cuts at: 3701 3911 3701
Size: 210 5470
SbfI CC_TGCA'GG
Cuts at: 2725 2725
Size: 5680
ScaI AGT'ACT
Cuts at: 357 5197 357
Size: 4840 840
SexAI A'CCwGG_T
Cuts at: 2352 2352
Size: 5680
SfcI C'TryA_G
Cuts at: 33 553 2721 3406 4401 4592 33
Size: 520 2168 685 995 191 1121
Fragments arranged by size:
2168 1121 995 685 520 191
SfoI GGC'GCC
Cuts at: 3110 3358 3110
Size: 248 5432
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5680
SmaI CCC'GGG
Cuts at: 2700 2700
Size: 5680
SmlI C'TyrA_G
Cuts at: 591 749 1356 1666 2833 3748 4242 4504 591
Size: 158 607 310 1167 915 494 262 1767
Fragments arranged by size:
1767 1167 915 607 494 310 262 158
SnaBI TAC'GTA
Cuts at: 2670 2670
Size: 5680
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5680
SphI G_CATG'C
Cuts at: 2731 3231 2731
Size: 500 5180
SspI AAT'ATT
Cuts at: 957 957
Size: 5680
StuI AGG'CCT
Cuts at: 206 206
Size: 5680
StyI C'CwwG_G
Cuts at: 646 735 969 2812 3166 646
Size: 89 234 1843 354 3160
Fragments arranged by size:
3160 1843 354 234 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1132 1243 2176 3261 3394 3853 4064 1132
Size: 111 933 1085 133 459 211 2748
Fragments arranged by size:
2748 1085 933 459 211 133 111
TatI w'GTAC_w
Cuts at: 355 5195 355
Size: 4840 840
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5197 5229 5197
Size: 32 5648
Tth111I GACn'n_nGTC
Cuts at: 890 3475 890
Size: 2585 3095
XbaI T'CTAG_A
Cuts at: 2709 2709
Size: 5680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 456 3173 456
Size: 2717 2963
XmaI C'CCGG_G
Cuts at: 2698 2698
Size: 5680
XmnI GAAnn'nnTTC
Cuts at: 306 744 2241 306
Size: 438 1497 3745
Fragments arranged by size:
3745 1497 438
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PciI PflMI PmeI
PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BcgI BfrBI BglI BmtI
BsaXI BsiWI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PspXI SacII SanDI SfiI SgrAI
SrfI SwaI XhoI ZraI