Restriction map for
pF1KSDA0724
(Circular) (Six-base) MAPSORT of: pF1KSDA0724.seq Check: 6370 from: 1 to: 6028
pF1KSDA0724 6028 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1266 1266
Size: 6028
AatII G_ACGT'C
Cuts at: 1571 1571
Size: 6028
AccI GT'mk_AC
Cuts at: 1268 1292 3064 1268
Size: 24 1772 4232
Fragments arranged by size:
4232 1772 24
Acc65I G'GTAC_C
Cuts at: 336 3042 336
Size: 2706 3322
AclI AA'CG_TT
Cuts at: 5868 5868
Size: 6028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 2548 2799 2886 3851 4283 5032 5300 291
Size: 2257 251 87 965 432 749 268 1019
Fragments arranged by size:
2257 1019 965 749 432 268 251 87
AflII C'TTAA_G
Cuts at: 1945 1945
Size: 6028
AflIII A'CryG_T
Cuts at: 3378 4484 5461 3378
Size: 1106 977 3945
Fragments arranged by size:
3945 1106 977
AgeI A'CCGG_T
Cuts at: 4416 4416
Size: 6028
AhdI GACnn_n'nnGTC
Cuts at: 2374 2374
Size: 6028
AleI CACnn'nnGTG
Cuts at: 2304 2973 2304
Size: 669 5359
AlwNI CAG_nnn'CTG
Cuts at: 163 321 1648 1905 1986 2526 3331 4900 163
Size: 158 1327 257 81 540 805 1569 1291
Fragments arranged by size:
1569 1327 1291 805 540 257 158 81
ApaI G_GGCC'C
Cuts at: 2474 2474
Size: 6028
ApaLI G'TGCA_C
Cuts at: 4798 4798
Size: 6028
ApoI r'AATT_y
Cuts at: 1388 3030 5381 5637 1388
Size: 1642 2351 256 1779
Fragments arranged by size:
2351 1779 1642 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6028
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6028
AvaI C'yCGr_G
Cuts at: 1085 3046 1085
Size: 1961 4067
AvrII C'CTAG_G
Cuts at: 1737 1737
Size: 6028
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 661 694 661
Size: 33 5995
BamHI G'GATC_C
Cuts at: 3051 3051
Size: 6028
BanI G'GyrC_C
Cuts at: 336 825 1587 2295 2616 3042 3456 3704 3739
Size: 489 762 708 321 426 414 248 35
Cuts at: 3739 336
Size: 2625
Fragments arranged by size:
2625 762 708 489 426 414 321 248
35
BanII G_rGCy'C
Cuts at: 890 1505 1598 1671 2474 3040 4070 890
Size: 615 93 73 803 566 1030 2848
Fragments arranged by size:
2848 1030 803 615 566 93 73
BbeI G_GCGC'C
Cuts at: 3460 3708 3460
Size: 248 5780
BbsI GAAGACnn'nnnn_
Cuts at: 720 2169 2711 720
Size: 1449 542 4037
Fragments arranged by size:
4037 1449 542
BbvCI CC'TCA_GC
Cuts at: 581 2878 581
Size: 2297 3731
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2899 2933 2899
Size: 34 5994
BciVI GTATCCnnnnn_n'
Cuts at: 2889 3924 4687 6028 2889
Size: 1035 763 1341 2889
Fragments arranged by size:
2889 1341 1035 763
BclI T'GATC_A
Cuts at: 1703 2100 3546 1703
Size: 397 1446 4185
Fragments arranged by size:
4185 1446 397
BglI GCCn_nnn'nGGC
Cuts at: 514 514
Size: 6028
BglII A'GATC_T
Cuts at: 380 1784 3541 5580 380
Size: 1404 1757 2039 828
Fragments arranged by size:
2039 1757 1404 828
BlpI GC'TnA_GC
Cuts at: 1453 2200 3138 1453
Size: 747 938 4343
Fragments arranged by size:
4343 938 747
Bme1580I G_kGCm'C
Cuts at: 2474 3651 3744 4802 2474
Size: 1177 93 1058 3700
Fragments arranged by size:
3700 1177 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 669 1323 3238 3319 3654 5800 669
Size: 654 1915 81 335 2146 897
Fragments arranged by size:
2146 1915 897 654 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1098 1524 4393 1098
Size: 426 2869 2733
Fragments arranged by size:
2869 2733 426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 414 760 789 841 2008 2755 185
Size: 229 346 29 52 1167 747 3458
Fragments arranged by size:
3458 1167 747 346 229 52 29
Bpu10I CC'TnA_GC
Cuts at: 581 1748 2114 2824 2878 581
Size: 1167 366 710 54 3731
Fragments arranged by size:
3731 1167 710 366 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 1762 2587 3202 4081 4575 4873 778
Size: 984 825 615 879 494 298 1933
Fragments arranged by size:
1933 984 879 825 615 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 291 1027 1180 1552 2989 5732 291
Size: 736 153 372 1437 2743 587
Fragments arranged by size:
2743 1437 736 587 372 153
BsaAI yAC'GTr
Cuts at: 951 3018 3379 951
Size: 2067 361 3600
Fragments arranged by size:
3600 2067 361
BsaBI GATnn'nnATC
Cuts at: 6 1621 3540 5579 6
Size: 1615 1919 2039 455
Fragments arranged by size:
2039 1919 1615 455
BsaHI Gr'CG_yC
Cuts at: 1568 3457 3705 4409 5905 1568
Size: 1889 248 704 1496 1691
Fragments arranged by size:
1889 1691 1496 704 248
BsaWI w'CCGG_w
Cuts at: 3215 3736 4416 4690 4837 5248 3215
Size: 521 680 274 147 411 3995
Fragments arranged by size:
3995 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2224 2254 2224
Size: 30 5998
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 664 1513 1522 1951 2017 154
Size: 510 849 9 429 66 4165
Fragments arranged by size:
4165 849 510 429 66 9
BseYI C'CCAG_C
Cuts at: 760 769 809 984 1473 1905 2316 3451 4209
Size: 9 40 175 489 432 411 1135 758
Cuts at: 4209 4788 5284 760
Size: 579 496 1504
Fragments arranged by size:
1504 1135 758 579 496 489 432 411
175 40 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 835 1036 1137 1303 1654 1939 199
Size: 636 201 101 166 351 285 4288
Fragments arranged by size:
4288 636 351 285 201 166 101
BsiEI CG_ry'CG
Cuts at: 104 2712 3568 3614 4824 104
Size: 2608 856 46 1210 1308
Fragments arranged by size:
2608 1308 1210 856 46
BsiHKAI G_wGCw'C
Cuts at: 369 794 890 1112 1671 3040 3818 4008 4802
Size: 425 96 222 559 1369 778 190 794
Cuts at: 4802 369
Size: 1595
Fragments arranged by size:
1595 1369 794 778 559 425 222 190
96
BsmI GAATG_Cn'
Cuts at: 924 924
Size: 6028
Bsp1286I G_dGCh'C
Cuts at: 369 794 890 1112 1505 1598 1671 2474 3040
Size: 425 96 222 393 93 73 803 566
Cuts at: 3040 3651 3744 3818 4008 4070 4802 369
Size: 611 93 74 190 62 732 1595
Fragments arranged by size:
1595 803 732 611 566 425 393 222
190 96 93 93 74 73 62
BspEI T'CCGG_A
Cuts at: 5248 5248
Size: 6028
BspHI T'CATG_A
Cuts at: 1700 2091 2103 2391 5204 1700
Size: 391 12 288 2813 2524
Fragments arranged by size:
2813 2524 391 288 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1266 1817 1961 2163 3076 3290 3592 3973 1266
Size: 551 144 202 913 214 302 381 3321
Fragments arranged by size:
3321 913 551 381 302 214 202 144
BsrBI CCG'CTC
Cuts at: 50 132 4318 4372 5858 6023 50
Size: 82 4186 54 1486 165 55
Fragments arranged by size:
4186 1486 165 82 55 54
BsrDI GCAATG_nn'
Cuts at: 3938 3938
Size: 6028
BsrFI r'CCGG_y
Cuts at: 1283 4024 4205 4416 1283
Size: 2741 181 211 2895
Fragments arranged by size:
2895 2741 211 181
BsrGI T'GTAC_A
Cuts at: 2327 2327
Size: 6028
BssSI C'ACGA_G
Cuts at: 4297 4657 4297
Size: 360 5668
BstAPI GCAn_nnn'nTGC
Cuts at: 321 1014 3403 321
Size: 693 2389 2946
Fragments arranged by size:
2946 2389 693
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6028
BstXI CCAn_nnnn'nTGG
Cuts at: 299 355 1833 2028 2154 299
Size: 56 1478 195 126 4173
Fragments arranged by size:
4173 1478 195 126 56
BstYI r'GATC_y
Cuts at: 1 344 380 1784 1865 1940 2912 3051 3518
Size: 343 36 1404 81 75 972 139 467
Cuts at: 3518 3541 3876 4122 5136 5222 5234 5580 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
1404 1014 972 467 449 346 343 335
246 139 86 81 75 36 23 12
BstZ17I GTA'TAC
Cuts at: 1269 1269
Size: 6028
Bsu36I CC'TnA_GG
Cuts at: 647 2612 647
Size: 1965 4063
BtgI C'CryG_G
Cuts at: 125 1041 1612 2153 2291 2988 125
Size: 916 571 541 138 697 3165
Fragments arranged by size:
3165 916 697 571 541 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1041 1536 3976 4157 4320 1041
Size: 495 2440 181 163 2749
Fragments arranged by size:
2749 2440 495 181 163
BtsI GCAGTG_nn'
Cuts at: 906 1019 1222 2735 2808 2968 3269 906
Size: 113 203 1513 73 160 301 3665
Fragments arranged by size:
3665 1513 301 203 160 113 73
DraI TTT'AAA
Cuts at: 3025 3025
Size: 6028
DrdI GACnn_nn'nnGTC
Cuts at: 1696 3732 4592 1696
Size: 2036 860 3132
Fragments arranged by size:
3132 2036 860
EaeI y'GGCC_r
Cuts at: 541 1167 1285 2030 3611 3785 4176 4203 5550
Size: 626 118 745 1581 174 391 27 1347
Cuts at: 5550 541
Size: 1019
Fragments arranged by size:
1581 1347 1019 745 626 391 174 118
27
EagI C'GGCC_G
Cuts at: 3611 3611
Size: 6028
EarI CTCTTCn'nnn_
Cuts at: 374 445 577 895 1767 1931 2071 2431 2464
Size: 71 132 318 872 164 140 360 33
Cuts at: 2464 4049 4259 374
Size: 1585 210 2143
Fragments arranged by size:
2143 1585 872 360 318 210 164 140
132 71 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4234 4546 4692 5838 4234
Size: 312 146 1146 4424
Fragments arranged by size:
4424 1146 312 146
EcoICRI GAG'CTC
Cuts at: 888 1669 3038 888
Size: 781 1369 3878
Fragments arranged by size:
3878 1369 781
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 291 414 760 789 841 2008 2548 2755
Size: 106 123 346 29 52 1167 540 207
Cuts at: 2755 2799 2886 3851 4283 5032 5300 185
Size: 44 87 965 432 749 268 913
Fragments arranged by size:
1167 965 913 749 540 432 346 268
207 123 106 87 52 44 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2518 2518
Size: 6028
EcoO109I rG'GnC_Cy
Cuts at: 1527 2110 2143 2421 2470 2844 3165 1527
Size: 583 33 278 49 374 321 4390
Fragments arranged by size:
4390 583 374 321 278 49 33
EcoRI G'AATT_C
Cuts at: 3030 3030
Size: 6028
EcoRV GAT'ATC
Cuts at: 2787 2787
Size: 6028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2829 2916 4045 2829
Size: 87 1129 4812
Fragments arranged by size:
4812 1129 87
FspI TGC'GCA
Cuts at: 3807 3807
Size: 6028
HaeII r_GCGC'y
Cuts at: 214 850 1906 3460 3708 4732 5711 214
Size: 636 1056 1554 248 1024 979 531
Fragments arranged by size:
1554 1056 1024 979 636 531 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1225 1257 1998 2030 2304 2336 2360 2392 2552
Size: 32 741 32 274 32 24 32 160
Cuts at: 2552 2584 3868 3898 3900 3930 1225
Size: 32 1284 30 2 30 3323
Fragments arranged by size:
3323 1284 741 274 160 32 32 32
32 32 30 30 24 2
HincII GTy'rAC
Cuts at: 1137 1572 3065 1137
Size: 435 1493 4100
Fragments arranged by size:
4100 1493 435
KasI G'GCGC_C
Cuts at: 3456 3704 3456
Size: 248 5780
KpnI G_GTAC'C
Cuts at: 340 3046 340
Size: 2706 3322
MfeI C'AATT_G
Cuts at: 2414 2414
Size: 6028
MluI A'CGCG_T
Cuts at: 5461 5461
Size: 6028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2045 2084 2302 4699 4883 2045
Size: 39 218 2397 184 3190
Fragments arranged by size:
3190 2397 218 184 39
MscI TGG'CCA
Cuts at: 543 1169 2032 3787 543
Size: 626 863 1755 2784
Fragments arranged by size:
2784 1755 863 626
MslI CAynn'nnrTG
Cuts at: 1060 1144 1417 1651 2096 2304 2728 2973 4015
Size: 84 273 234 445 208 424 245 1042
Cuts at: 4015 4142 1060
Size: 127 2946
Fragments arranged by size:
2946 1042 445 424 273 245 234 208
127 84
MspA1I CmG'CkG
Cuts at: 311 611 735 747 773 809 819 831 978
Size: 300 124 12 26 36 10 12 147
Cuts at: 978 1302 1638 2304 2373 3007 3137 3451 3811
Size: 324 336 666 69 634 130 314 360
Cuts at: 3811 4826 5071 311
Size: 1015 245 1268
Fragments arranged by size:
1268 1015 666 634 360 336 324 314
300 245 147 130 124 69 36 26
12 12 10
NaeI GCC'GGC
Cuts at: 4207 4207
Size: 6028
NarI GG'CG_CC
Cuts at: 3457 3705 3457
Size: 248 5780
NcoI C'CATG_G
Cuts at: 125 1041 2291 2988 125
Size: 916 1250 697 3165
Fragments arranged by size:
3165 1250 916 697
NgoMIV G'CCGG_C
Cuts at: 4205 4205
Size: 6028
NruI TCG'CGA
Cuts at: 2921 4389 2921
Size: 1468 4560
NspI r_CATG'y
Cuts at: 1735 1890 2832 3079 3579 4488 1735
Size: 155 942 247 500 909 3275
Fragments arranged by size:
3275 942 909 500 247 155
PasI CC'CwG_GG
Cuts at: 1068 2474 1068
Size: 1406 4622
PciI A'CATG_T
Cuts at: 4484 4484
Size: 6028
PflMI CCAn_nnn'nTGG
Cuts at: 598 1305 1986 2229 598
Size: 707 681 243 4397
Fragments arranged by size:
4397 707 681 243
PmeI GTTT'AAAC
Cuts at: 3025 3025
Size: 6028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1942 1974 5167 5199 1942
Size: 32 3193 32 2771
Fragments arranged by size:
3193 2771 32 32
PpuMI rG'GwC_Cy
Cuts at: 2143 2143
Size: 6028
PshAI GACnn'nnGTC
Cuts at: 2084 2084
Size: 6028
PspOMI G'GGCC_C
Cuts at: 2470 2470
Size: 6028
PstI C_TGCA'G
Cuts at: 159 326 836 878 995 1229 2171 2852 3073
Size: 167 510 42 117 234 942 681 221
Cuts at: 3073 3758 159
Size: 685 2429
Fragments arranged by size:
2429 942 685 681 510 234 221 167
117 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6028
PvuII CAG'CTG
Cuts at: 311 747 773 809 819 1302 1638 2304 2373
Size: 436 26 36 10 483 336 666 69
Cuts at: 2373 3007 3451 3811 311
Size: 634 444 360 2528
Fragments arranged by size:
2528 666 634 483 444 436 360 336
69 36 26 10
RsrII CG'GwC_CG
Cuts at: 4221 4221
Size: 6028
SacI G_AGCT'C
Cuts at: 890 1671 3040 890
Size: 781 1369 3878
Fragments arranged by size:
3878 1369 781
SalI G'TCGA_C
Cuts at: 3063 3063
Size: 6028
SanDI GG'GwC_CC
Cuts at: 2143 2143
Size: 6028
SapI GCTCTTCn'nnn_
Cuts at: 374 445 895 1931 4049 4259 374
Size: 71 450 1036 2118 210 2143
Fragments arranged by size:
2143 2118 1036 450 210 71
SbfI CC_TGCA'GG
Cuts at: 3073 3073
Size: 6028
ScaI AGT'ACT
Cuts at: 352 5545 352
Size: 5193 835
SexAI A'CCwGG_T
Cuts at: 2856 2856
Size: 6028
SfcI C'TryA_G
Cuts at: 33 155 322 751 832 874 991 1225 2167
Size: 122 167 429 81 42 117 234 942
Cuts at: 2167 2848 3069 3754 4749 4940 33
Size: 681 221 685 995 191 1121
Fragments arranged by size:
1121 995 942 685 681 429 234 221
191 167 122 117 81 42
SfoI GGC'GCC
Cuts at: 3458 3706 3458
Size: 248 5780
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6028
SmaI CCC'GGG
Cuts at: 1087 3048 1087
Size: 1961 4067
SmlI C'TyrA_G
Cuts at: 793 1777 1945 2602 3181 4096 4590 4852 793
Size: 984 168 657 579 915 494 262 1969
Fragments arranged by size:
1969 984 915 657 579 494 262 168
SnaBI TAC'GTA
Cuts at: 3018 3018
Size: 6028
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6028
SphI G_CATG'C
Cuts at: 1735 3079 3579 1735
Size: 1344 500 4184
Fragments arranged by size:
4184 1344 500
StuI AGG'CCT
Cuts at: 298 689 2347 2632 2755 298
Size: 391 1658 285 123 3571
Fragments arranged by size:
3571 1658 391 285 123
StyI C'CwwG_G
Cuts at: 125 179 1041 1721 1737 2291 2988 3160 3514
Size: 54 862 680 16 554 697 172 354
Cuts at: 3514 125
Size: 2639
Fragments arranged by size:
2639 862 697 680 554 354 172 54
16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 460 783 801 3609 3742 4201 4412 460
Size: 323 18 2808 133 459 211 2076
Fragments arranged by size:
2808 2076 459 323 211 133 18
TatI w'GTAC_w
Cuts at: 350 480 2327 5543 350
Size: 130 1847 3216 835
Fragments arranged by size:
3216 1847 835 130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1961 1993 2224 2256 2846 2878 5545 5577 1961
Size: 32 231 32 590 32 2667 32 2412
Fragments arranged by size:
2667 2412 590 231 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1648 3823 1648
Size: 2175 3853
XbaI T'CTAG_A
Cuts at: 3057 3057
Size: 6028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 708 2154 3521 708
Size: 1446 1367 3215
Fragments arranged by size:
3215 1446 1367
XmaI C'CCGG_G
Cuts at: 1085 3046 1085
Size: 1961 4067
XmnI GAAnn'nnTTC
Cuts at: 2962 2962
Size: 6028
ZraI GAC'GTC
Cuts at: 1569 1569
Size: 6028
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AlfI AloI AscI BfrBI BmgBI BmtI BsiWI
BsmBI BssHII BstEII ClaI CspCI DraIII FseI FspAI
HindIII HpaI NdeI NheI NotI NsiI PacI PfoI
PmlI PsiI PspXI PsrI SacII SfiI SgrAI SrfI
SspI SwaI XhoI