Restriction map for
pF1KSDA0733
(Circular) (Six-base) MAPSORT of: pF1KSDA0733.seq Check: 963 from: 1 to: 5218
pF1KSDA0733 5218 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 336 974 2254 336
Size: 638 1280 3300
Fragments arranged by size:
3300 1280 638
Acc65I G'GTAC_C
Cuts at: 2232 2232
Size: 5218
AclI AA'CG_TT
Cuts at: 5058 5058
Size: 5218
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 218 1169 3041 3473 4222 4490 209
Size: 9 951 1872 432 749 268 937
Fragments arranged by size:
1872 951 937 749 432 268 9
AfeI AGC'GCT
Cuts at: 2004 2004
Size: 5218
AflIII A'CryG_T
Cuts at: 1388 1662 2568 3674 4651 1388
Size: 274 906 1106 977 1955
Fragments arranged by size:
1955 1106 977 906 274
AgeI A'CCGG_T
Cuts at: 3606 3606
Size: 5218
AhdI GACnn_n'nnGTC
Cuts at: 899 899
Size: 5218
AlwNI CAG_nnn'CTG
Cuts at: 1908 2521 4090 1908
Size: 613 1569 3036
Fragments arranged by size:
3036 1569 613
ApaLI G'TGCA_C
Cuts at: 3988 3988
Size: 5218
ApoI r'AATT_y
Cuts at: 181 306 326 573 756 1866 2220 4571 4827
Size: 125 20 247 183 1110 354 2351 256
Cuts at: 4827 181
Size: 572
Fragments arranged by size:
2351 1110 572 354 256 247 183 125
20
AseI AT'TA_AT
Cuts at: 20 20
Size: 5218
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5218
AvaI C'yCGr_G
Cuts at: 1478 1979 2236 1478
Size: 501 257 4460
Fragments arranged by size:
4460 501 257
BamHI G'GATC_C
Cuts at: 2241 2241
Size: 5218
BanI G'GyrC_C
Cuts at: 1850 2232 2646 2894 2929 1850
Size: 382 414 248 35 4139
Fragments arranged by size:
4139 414 382 248 35
BanII G_rGCy'C
Cuts at: 2124 2230 3260 2124
Size: 106 1030 4082
Fragments arranged by size:
4082 1030 106
BbeI G_GCGC'C
Cuts at: 1854 2650 2898 1854
Size: 796 248 4174
Fragments arranged by size:
4174 796 248
BbsI GAAGACnn'nnnn_
Cuts at: 1034 1034
Size: 5218
BbvCI CC'TCA_GC
Cuts at: 857 857
Size: 5218
BciVI GTATCCnnnnn_n'
Cuts at: 219 3114 3877 5218 219
Size: 2895 763 1341 219
Fragments arranged by size:
2895 1341 763 219
BclI T'GATC_A
Cuts at: 1608 2736 1608
Size: 1128 4090
BglII A'GATC_T
Cuts at: 2731 4770 2731
Size: 2039 3179
BlpI GC'TnA_GC
Cuts at: 2328 2328
Size: 5218
Bme1580I G_kGCm'C
Cuts at: 2841 2934 3992 2841
Size: 93 1058 4067
Fragments arranged by size:
4067 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 2428 2509 2844 4990 2428
Size: 81 335 2146 2656
Fragments arranged by size:
2656 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3583 3583
Size: 5218
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1155 1365 1385 1915 1155
Size: 210 20 530 4458
Fragments arranged by size:
4458 530 210 20
Bpu10I CC'TnA_GC
Cuts at: 857 857
Size: 5218
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 512 1798 2081 2392 3271 3765 4063 503
Size: 9 1286 283 311 879 494 298 1658
Fragments arranged by size:
1658 1286 879 494 311 298 283 9
BsaI GGTCTCn'nnnn_
Cuts at: 4922 4922
Size: 5218
BsaAI yAC'GTr
Cuts at: 2208 2569 2208
Size: 361 4857
BsaBI GATnn'nnATC
Cuts at: 6 1105 2730 4769 6
Size: 1099 1625 2039 455
Fragments arranged by size:
2039 1625 1099 455
BsaHI Gr'CG_yC
Cuts at: 1851 2647 2895 3599 5095 1851
Size: 796 248 704 1496 1974
Fragments arranged by size:
1974 1496 796 704 248
BsaWI w'CCGG_w
Cuts at: 2405 2926 3606 3880 4027 4438 2405
Size: 521 680 274 147 411 3185
Fragments arranged by size:
3185 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1515 1545 1515
Size: 30 5188
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1466 1466
Size: 5218
BseYI C'CCAG_C
Cuts at: 827 1999 2641 3399 3978 4474 827
Size: 1172 642 758 579 496 1571
Fragments arranged by size:
1571 1172 758 642 579 496
BsiEI CG_ry'CG
Cuts at: 104 2758 2804 4014 104
Size: 2654 46 1210 1308
Fragments arranged by size:
2654 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 2124 2230 3008 3198 3992 2124
Size: 106 778 190 794 3350
Fragments arranged by size:
3350 794 778 190 106
BsmI GAATG_Cn'
Cuts at: 819 1305 819
Size: 486 4732
Bsp1286I G_dGCh'C
Cuts at: 2124 2230 2841 2934 3008 3198 3260 3992 2124
Size: 106 611 93 74 190 62 732 3350
Fragments arranged by size:
3350 732 611 190 106 93 74 62
BspEI T'CCGG_A
Cuts at: 4438 4438
Size: 5218
BspHI T'CATG_A
Cuts at: 4394 4394
Size: 5218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 179 2266 2480 2782 3163 179
Size: 2087 214 302 381 2234
Fragments arranged by size:
2234 2087 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3508 3562 5048 5213 50
Size: 3458 54 1486 165 55
Fragments arranged by size:
3458 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3128 3128
Size: 5218
BsrFI r'CCGG_y
Cuts at: 3214 3395 3606 3214
Size: 181 211 4826
Fragments arranged by size:
4826 211 181
BssSI C'ACGA_G
Cuts at: 3487 3847 3487
Size: 360 4858
BstAPI GCAn_nnn'nTGC
Cuts at: 1375 2593 1375
Size: 1218 4000
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5218
BstYI r'GATC_y
Cuts at: 1 1702 2241 2708 2731 3066 3312 4326 4412
Size: 1701 539 467 23 335 246 1014 86
Cuts at: 4412 4424 4770 1
Size: 12 346 449
Fragments arranged by size:
1701 1014 539 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1280 1280
Size: 5218
BtgI C'CryG_G
Cuts at: 125 393 125
Size: 268 4950
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3166 3347 3510 3166
Size: 181 163 4874
Fragments arranged by size:
4874 181 163
BtsI GCAGTG_nn'
Cuts at: 2459 2459
Size: 5218
DraI TTT'AAA
Cuts at: 2215 2215
Size: 5218
DrdI GACnn_nn'nnGTC
Cuts at: 2922 3782 2922
Size: 860 4358
EaeI y'GGCC_r
Cuts at: 468 2801 2975 3366 3393 4740 468
Size: 2333 174 391 27 1347 946
Fragments arranged by size:
2333 1347 946 391 174 27
EagI C'GGCC_G
Cuts at: 2801 2801
Size: 5218
EarI CTCTTCn'nnn_
Cuts at: 393 1188 1351 3239 3449 393
Size: 795 163 1888 210 2162
Fragments arranged by size:
2162 1888 795 210 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3424 3736 3882 5028 3424
Size: 312 146 1146 3614
Fragments arranged by size:
3614 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2122 2228 2122
Size: 106 5112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 218 1155 1169 1365 1385 1915 3041 3473
Size: 9 937 14 196 20 530 1126 432
Cuts at: 3473 4222 4490 209
Size: 749 268 937
Fragments arranged by size:
1126 937 937 749 530 432 268 196
20 14 9
EcoO109I rG'GnC_Cy
Cuts at: 1084 1411 2041 2355 1084
Size: 327 630 314 3947
Fragments arranged by size:
3947 630 327 314
EcoRI G'AATT_C
Cuts at: 2220 2220
Size: 5218
EcoRV GAT'ATC
Cuts at: 283 283
Size: 5218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3235 3235
Size: 5218
FspI TGC'GCA
Cuts at: 2997 2997
Size: 5218
HaeII r_GCGC'y
Cuts at: 1854 2006 2650 2898 3922 4901 1854
Size: 152 644 248 1024 979 2171
Fragments arranged by size:
2171 1024 979 644 248 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 358 390 885 917 1249 1281 1395 1427 1472
Size: 32 495 32 332 32 114 32 45
Cuts at: 1472 1504 2056 2088 3058 3088 3090 3120 358
Size: 32 552 32 970 30 2 30 2456
Fragments arranged by size:
2456 970 552 495 332 114 45 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1276 2255 1276
Size: 979 4239
KasI G'GCGC_C
Cuts at: 1850 2646 2894 1850
Size: 796 248 4174
Fragments arranged by size:
4174 796 248
KpnI G_GTAC'C
Cuts at: 2236 2236
Size: 5218
MluI A'CGCG_T
Cuts at: 4651 4651
Size: 5218
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3889 4073 3889
Size: 184 5034
MscI TGG'CCA
Cuts at: 470 2977 470
Size: 2507 2711
MslI CAynn'nnrTG
Cuts at: 716 3205 3332 716
Size: 2489 127 2602
Fragments arranged by size:
2602 2489 127
MspA1I CmG'CkG
Cuts at: 1908 2327 2641 3001 4016 4261 1908
Size: 419 314 360 1015 245 2865
Fragments arranged by size:
2865 1015 419 360 314 245
NaeI GCC'GGC
Cuts at: 3397 3397
Size: 5218
NarI GG'CG_CC
Cuts at: 1851 2647 2895 1851
Size: 796 248 4174
Fragments arranged by size:
4174 796 248
NcoI C'CATG_G
Cuts at: 125 393 125
Size: 268 4950
NgoMIV G'CCGG_C
Cuts at: 3395 3395
Size: 5218
NruI TCG'CGA
Cuts at: 3579 3579
Size: 5218
NspI r_CATG'y
Cuts at: 222 981 1392 1666 2269 2769 3678 222
Size: 759 411 274 603 500 909 1762
Fragments arranged by size:
1762 909 759 603 500 411 274
PciI A'CATG_T
Cuts at: 1388 1662 3674 1388
Size: 274 2012 2932
Fragments arranged by size:
2932 2012 274
PflMI CCAn_nnn'nTGG
Cuts at: 589 589
Size: 5218
PfoI T'CCnGG_A
Cuts at: 1381 1381
Size: 5218
PmeI GTTT'AAAC
Cuts at: 2215 2215
Size: 5218
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4357 4389 4357
Size: 32 5186
PpuMI rG'GwC_Cy
Cuts at: 1084 1411 2041 1084
Size: 327 630 4261
Fragments arranged by size:
4261 630 327
PspXI vC'TCGA_Gb
Cuts at: 1478 1478
Size: 5218
PstI C_TGCA'G
Cuts at: 1551 2263 2948 1551
Size: 712 685 3821
Fragments arranged by size:
3821 712 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5218
PvuII CAG'CTG
Cuts at: 1908 2641 3001 1908
Size: 733 360 4125
Fragments arranged by size:
4125 733 360
RsrII CG'GwC_CG
Cuts at: 3411 3411
Size: 5218
SacI G_AGCT'C
Cuts at: 2124 2230 2124
Size: 106 5112
SalI G'TCGA_C
Cuts at: 2253 2253
Size: 5218
SapI GCTCTTCn'nnn_
Cuts at: 3239 3449 3239
Size: 210 5008
SbfI CC_TGCA'GG
Cuts at: 2263 2263
Size: 5218
ScaI AGT'ACT
Cuts at: 4735 4735
Size: 5218
SfcI C'TryA_G
Cuts at: 33 1547 2259 2944 3939 4130 33
Size: 1514 712 685 995 191 1121
Fragments arranged by size:
1514 1121 995 712 685 191
SfoI GGC'GCC
Cuts at: 1852 2648 2896 1852
Size: 796 248 4174
Fragments arranged by size:
4174 796 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5218
SmaI CCC'GGG
Cuts at: 2238 2238
Size: 5218
SmlI C'TyrA_G
Cuts at: 518 527 1478 1777 2096 2371 3286 3780 4042
Size: 9 951 299 319 275 915 494 262
Cuts at: 4042 518
Size: 1694
Fragments arranged by size:
1694 951 915 494 319 299 275 262
9
SnaBI TAC'GTA
Cuts at: 2208 2208
Size: 5218
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5218
SphI G_CATG'C
Cuts at: 2269 2769 2269
Size: 500 4718
SspI AAT'ATT
Cuts at: 1074 1632 2028 1074
Size: 558 396 4264
Fragments arranged by size:
4264 558 396
StuI AGG'CCT
Cuts at: 774 774
Size: 5218
StyI C'CwwG_G
Cuts at: 125 132 393 2194 2350 2704 125
Size: 7 261 1801 156 354 2639
Fragments arranged by size:
2639 1801 354 261 156 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2799 2932 3391 3602 2799
Size: 133 459 211 4415
Fragments arranged by size:
4415 459 211 133
TatI w'GTAC_w
Cuts at: 274 735 2143 4733 274
Size: 461 1408 2590 759
Fragments arranged by size:
2590 1408 759 461
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4735 4767 4735
Size: 32 5186
Tth111I GACn'n_nGTC
Cuts at: 3013 3013
Size: 5218
XbaI T'CTAG_A
Cuts at: 2247 2247
Size: 5218
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2711 2711
Size: 5218
XhoI C'TCGA_G
Cuts at: 1478 1478
Size: 5218
XmaI C'CCGG_G
Cuts at: 2236 2236
Size: 5218
XmnI GAAnn'nnTTC
Cuts at: 488 1134 1763 488
Size: 646 629 3943
Fragments arranged by size:
3943 646 629
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NsiI PacI PasI PmlI PshAI
PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SwaI ZraI