Restriction map for
pF1KSDA0735
(Circular) (Six-base) MAPSORT of: pF1KSDA0735.seq Check: 3923 from: 1 to: 5186
pF1KSDA0735 5186 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 587 2224 587
Size: 1637 3549
Acc65I G'GTAC_C
Cuts at: 1202 2202 1202
Size: 1000 4186
AclI AA'CG_TT
Cuts at: 5026 5026
Size: 5186
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 903 1027 3011 3443 4190 4458 903
Size: 124 1984 432 747 268 1631
Fragments arranged by size:
1984 1631 747 432 268 124
AfeI AGC'GCT
Cuts at: 1210 1210
Size: 5186
AflIII A'CryG_T
Cuts at: 542 1521 2538 3642 4619 542
Size: 979 1017 1104 977 1109
Fragments arranged by size:
1109 1104 1017 979 977
AgeI A'CCGG_T
Cuts at: 3574 3574
Size: 5186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1878 1878
Size: 5186
AlwNI CAG_nnn'CTG
Cuts at: 1917 2169 2491 4058 1917
Size: 252 322 1567 3045
Fragments arranged by size:
3045 1567 322 252
ApaI G_GGCC'C
Cuts at: 1280 1280
Size: 5186
ApaLI G'TGCA_C
Cuts at: 3956 3956
Size: 5186
ApoI r'AATT_y
Cuts at: 799 1168 2029 2190 4539 4795 799
Size: 369 861 161 2349 256 1190
Fragments arranged by size:
2349 1190 861 369 256 161
AseI AT'TA_AT
Cuts at: 20 20
Size: 5186
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5186
AvaI C'yCGr_G
Cuts at: 809 1777 2156 2206 809
Size: 968 379 50 3789
Fragments arranged by size:
3789 968 379 50
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 264 297 264
Size: 33 5153
BamHI G'GATC_C
Cuts at: 452 2211 452
Size: 1759 3427
BanI G'GyrC_C
Cuts at: 324 607 1202 1435 2035 2202 2616 2864 2899
Size: 283 595 233 600 167 414 248 35
Cuts at: 2899 324
Size: 2611
Fragments arranged by size:
2611 600 595 414 283 248 233 167
35
BanII G_rGCy'C
Cuts at: 1027 1280 2200 3230 1027
Size: 253 920 1030 2983
Fragments arranged by size:
2983 1030 920 253
BbeI G_GCGC'C
Cuts at: 328 611 1439 2039 2620 2868 328
Size: 283 828 600 581 248 2646
Fragments arranged by size:
2646 828 600 581 283 248
BbsI GAAGACnn'nnnn_
Cuts at: 257 1571 257
Size: 1314 3872
BbvCI CC'TCA_GC
Cuts at: 658 658
Size: 5186
BciVI GTATCCnnnnn_n'
Cuts at: 292 1988 3084 3845 5186 292
Size: 1696 1096 761 1341 292
Fragments arranged by size:
1696 1341 1096 761 292
BclI T'GATC_A
Cuts at: 1961 2706 1961
Size: 745 4441
BglI GCCn_nnn'nGGC
Cuts at: 313 322 350 484 313
Size: 9 28 134 5015
Fragments arranged by size:
5015 134 28 9
BglII A'GATC_T
Cuts at: 257 2701 4738 257
Size: 2444 2037 705
Fragments arranged by size:
2444 2037 705
BlpI GC'TnA_GC
Cuts at: 2298 2298
Size: 5186
Bme1580I G_kGCm'C
Cuts at: 1280 2811 2904 3960 1280
Size: 1531 93 1056 2506
Fragments arranged by size:
2506 1531 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 164 521 869 2398 2479 2814 4958 164
Size: 357 348 1529 81 335 2144 392
Fragments arranged by size:
2144 1529 392 357 348 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 745 847 3553 745
Size: 102 2706 2378
Fragments arranged by size:
2706 2378 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 909 972 1705 315
Size: 594 63 733 3796
Fragments arranged by size:
3796 733 594 63
Bpu10I CC'TnA_GC
Cuts at: 658 1106 658
Size: 448 4738
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1807 2362 3241 3733 4031 1060
Size: 747 555 879 492 298 2215
Fragments arranged by size:
2215 879 747 555 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 406 4890 406
Size: 4484 702
BsaAI yAC'GTr
Cuts at: 2178 2539 2178
Size: 361 4825
BsaBI GATnn'nnATC
Cuts at: 6 2700 4737 6
Size: 2694 2037 455
Fragments arranged by size:
2694 2037 455
BsaHI Gr'CG_yC
Cuts at: 325 608 1436 2036 2617 2865 3567 5063 325
Size: 283 828 600 581 248 702 1496 448
Fragments arranged by size:
1496 828 702 600 581 448 283 248
BsaWI w'CCGG_w
Cuts at: 2375 2896 3574 3848 3995 4406 2375
Size: 521 678 274 147 411 3155
Fragments arranged by size:
3155 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 403 836 403
Size: 433 4753
BseYI C'CCAG_C
Cuts at: 639 840 911 2611 3369 3946 4442 639
Size: 201 71 1700 758 577 496 1383
Fragments arranged by size:
1700 1383 758 577 496 201 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 551 730 969 551
Size: 179 239 4768
Fragments arranged by size:
4768 239 179
BsiEI CG_ry'CG
Cuts at: 104 2728 2774 3982 104
Size: 2624 46 1208 1308
Fragments arranged by size:
2624 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 771 978 1646 2200 2978 3168 3960 771
Size: 207 668 554 778 190 792 1997
Fragments arranged by size:
1997 792 778 668 554 207 190
BsmI GAATG_Cn'
Cuts at: 575 1327 1953 2009 575
Size: 752 626 56 3752
Fragments arranged by size:
3752 752 626 56
Bsp1286I G_dGCh'C
Cuts at: 771 978 1027 1280 1646 2200 2811 2904 2978
Size: 207 49 253 366 554 611 93 74
Cuts at: 2978 3168 3230 3960 771
Size: 190 62 730 1997
Fragments arranged by size:
1997 730 611 554 366 253 207 190
93 74 62 49
BspEI T'CCGG_A
Cuts at: 4406 4406
Size: 5186
BspHI T'CATG_A
Cuts at: 4362 4362
Size: 5186
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1233 2236 2450 2752 3133 1233
Size: 1003 214 302 381 3286
Fragments arranged by size:
3286 1003 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3478 3532 5016 5181 50
Size: 3428 54 1484 165 55
Fragments arranged by size:
3428 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 198 1904 1934 3098 198
Size: 1706 30 1164 2286
Fragments arranged by size:
2286 1706 1164 30
BsrFI r'CCGG_y
Cuts at: 3184 3365 3574 3184
Size: 181 209 4796
Fragments arranged by size:
4796 209 181
BssSI C'ACGA_G
Cuts at: 3457 3815 3457
Size: 358 4828
BstAPI GCAn_nnn'nTGC
Cuts at: 2563 2563
Size: 5186
BstBI TT'CG_AA
Cuts at: 110 3547 110
Size: 3437 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1161 1477 1683 2100 1161
Size: 316 206 417 4247
Fragments arranged by size:
4247 417 316 206
BstYI r'GATC_y
Cuts at: 1 257 452 1178 1466 2211 2678 2701 3036
Size: 256 195 726 288 745 467 23 335
Cuts at: 3036 3282 4294 4380 4392 4738 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 745 726 467 449 346 335 288
256 246 195 86 23 12
BtgI C'CryG_G
Cuts at: 125 186 435 881 995 1307 1322 125
Size: 61 249 446 114 312 15 3989
Fragments arranged by size:
3989 446 312 249 114 61 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3136 3317 3480 3136
Size: 181 163 4842
Fragments arranged by size:
4842 181 163
BtsI GCAGTG_nn'
Cuts at: 1918 2429 1918
Size: 511 4675
DraI TTT'AAA
Cuts at: 1518 2185 1518
Size: 667 4519
DraIII CAC_nnn'GTG
Cuts at: 223 223
Size: 5186
DrdI GACnn_nn'nnGTC
Cuts at: 2892 3750 2892
Size: 858 4328
EaeI y'GGCC_r
Cuts at: 432 438 485 671 1304 2771 2945 3336 3363
Size: 6 47 186 633 1467 174 391 27
Cuts at: 3363 4708 432
Size: 1345 910
Fragments arranged by size:
1467 1345 910 633 391 186 174 47
27 6
EagI C'GGCC_G
Cuts at: 2771 2771
Size: 5186
EarI CTCTTCn'nnn_
Cuts at: 705 736 3209 3419 705
Size: 31 2473 210 2472
Fragments arranged by size:
2473 2472 210 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3394 3704 3850 4996 3394
Size: 310 146 1146 3584
Fragments arranged by size:
3584 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2198 2198
Size: 5186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 903 909 972 1027 1705 3011 3443 4190
Size: 588 6 63 55 678 1306 432 747
Cuts at: 4190 4458 315
Size: 268 1043
Fragments arranged by size:
1306 1043 747 678 588 432 268 63
55 6
EcoO109I rG'GnC_Cy
Cuts at: 864 1112 1276 1277 2166 2325 864
Size: 248 164 1 889 159 3725
Fragments arranged by size:
3725 889 248 164 159 1
EcoRI G'AATT_C
Cuts at: 799 1168 2190 799
Size: 369 1022 3795
Fragments arranged by size:
3795 1022 369
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3205 3205
Size: 5186
FspI TGC'GCA
Cuts at: 2967 2967
Size: 5186
HaeII r_GCGC'y
Cuts at: 328 611 1212 1439 2039 2620 2868 3890 4869
Size: 283 601 227 600 581 248 1022 979
Cuts at: 4869 328
Size: 645
Fragments arranged by size:
1022 979 645 601 600 581 283 248
227
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 947 979 1446 1478 1558 1590 2150 2182 3028
Size: 32 467 32 80 32 560 32 846
Cuts at: 3028 3058 3060 3090 947
Size: 30 2 30 3043
Fragments arranged by size:
3043 846 560 467 80 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2225 2225
Size: 5186
KasI G'GCGC_C
Cuts at: 324 607 1435 2035 2616 2864 324
Size: 283 828 600 581 248 2646
Fragments arranged by size:
2646 828 600 581 283 248
KpnI G_GTAC'C
Cuts at: 1206 2206 1206
Size: 1000 4186
MluI A'CGCG_T
Cuts at: 4619 4619
Size: 5186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 224 1163 3857 4041 224
Size: 939 2694 184 1369
Fragments arranged by size:
2694 1369 939 184
MscI TGG'CCA
Cuts at: 434 2947 434
Size: 2513 2673
MslI CAynn'nnrTG
Cuts at: 293 1096 1475 1507 3175 3302 293
Size: 803 379 32 1668 127 2177
Fragments arranged by size:
2177 1668 803 379 127 32
MspA1I CmG'CkG
Cuts at: 188 2297 2611 2971 3984 4229 188
Size: 2109 314 360 1013 245 1145
Fragments arranged by size:
2109 1145 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3367 3367
Size: 5186
NarI GG'CG_CC
Cuts at: 325 608 1436 2036 2617 2865 325
Size: 283 828 600 581 248 2646
Fragments arranged by size:
2646 828 600 581 283 248
NcoI C'CATG_G
Cuts at: 125 435 881 995 1322 125
Size: 310 446 114 327 3989
Fragments arranged by size:
3989 446 327 310 114
NgoMIV G'CCGG_C
Cuts at: 3365 3365
Size: 5186
NspI r_CATG'y
Cuts at: 546 666 1429 1525 2239 2739 3646 546
Size: 120 763 96 714 500 907 2086
Fragments arranged by size:
2086 907 763 714 500 120 96
PciI A'CATG_T
Cuts at: 542 1521 3642 542
Size: 979 2121 2086
Fragments arranged by size:
2121 2086 979
PflMI CCAn_nnn'nTGG
Cuts at: 887 907 887
Size: 20 5166
PfoI T'CCnGG_A
Cuts at: 1682 1682
Size: 5186
PmeI GTTT'AAAC
Cuts at: 2185 2185
Size: 5186
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1111 1143 1633 1665 4325 4357 1111
Size: 32 490 32 2660 32 1940
Fragments arranged by size:
2660 1940 490 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1112 2166 1112
Size: 1054 4132
PshAI GACnn'nnGTC
Cuts at: 545 545
Size: 5186
PspOMI G'GGCC_C
Cuts at: 1276 1276
Size: 5186
PstI C_TGCA'G
Cuts at: 1857 2233 2918 1857
Size: 376 685 4125
Fragments arranged by size:
4125 685 376
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5186
PvuII CAG'CTG
Cuts at: 2611 2971 2611
Size: 360 4826
RsrII CG'GwC_CG
Cuts at: 3381 3381
Size: 5186
SacI G_AGCT'C
Cuts at: 2200 2200
Size: 5186
SacII CC_GC'GG
Cuts at: 189 189
Size: 5186
SalI G'TCGA_C
Cuts at: 2223 2223
Size: 5186
SanDI GG'GwC_CC
Cuts at: 1112 1112
Size: 5186
SapI GCTCTTCn'nnn_
Cuts at: 3209 3419 3209
Size: 210 4976
SbfI CC_TGCA'GG
Cuts at: 2233 2233
Size: 5186
ScaI AGT'ACT
Cuts at: 4703 4703
Size: 5186
SexAI A'CCwGG_T
Cuts at: 1198 1198
Size: 5186
SfcI C'TryA_G
Cuts at: 33 1281 1853 2229 2914 3907 4098 33
Size: 1248 572 376 685 993 191 1121
Fragments arranged by size:
1248 1121 993 685 572 376 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 313 484 313
Size: 171 5015
SfoI GGC'GCC
Cuts at: 326 609 1437 2037 2618 2866 326
Size: 283 828 600 581 248 2646
Fragments arranged by size:
2646 828 600 581 283 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5186
SmaI CCC'GGG
Cuts at: 1779 2208 1779
Size: 429 4757
SmlI C'TyrA_G
Cuts at: 1075 1822 2156 2341 3256 3748 4010 1075
Size: 747 334 185 915 492 262 2251
Fragments arranged by size:
2251 915 747 492 334 262 185
SnaBI TAC'GTA
Cuts at: 2178 2178
Size: 5186
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5186
SphI G_CATG'C
Cuts at: 2239 2739 2239
Size: 500 4686
StuI AGG'CCT
Cuts at: 626 626
Size: 5186
StyI C'CwwG_G
Cuts at: 125 435 881 995 1027 1322 2320 2674 125
Size: 310 446 114 32 295 998 354 2637
Fragments arranged by size:
2637 998 446 354 310 295 114 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 305 2769 2902 3361 3570 305
Size: 2464 133 459 209 1921
Fragments arranged by size:
2464 1921 459 209 133
TatI w'GTAC_w
Cuts at: 1262 1511 1524 4701 1262
Size: 249 13 3177 1747
Fragments arranged by size:
3177 1747 249 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4703 4735 4703
Size: 32 5154
Tth111I GACn'n_nGTC
Cuts at: 2983 2983
Size: 5186
XbaI T'CTAG_A
Cuts at: 2217 2217
Size: 5186
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 908 2681 908
Size: 1773 3413
XhoI C'TCGA_G
Cuts at: 2156 2156
Size: 5186
XmaI C'CCGG_G
Cuts at: 1777 2206 1777
Size: 429 4757
XmnI GAAnn'nnTTC
Cuts at: 1126 1126
Size: 5186
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PsiI PspXI PsrI
SgrAI SrfI SspI SwaI ZraI