Restriction map for
pF1KSDA0738
(Circular) (Six-base) MAPSORT of: pF1KSDA0738.seq Check: 1123 from: 1 to: 5900
pF1KSDA0738 5900 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2467 2467
Size: 5900
AccI GT'mk_AC
Cuts at: 1124 2938 1124
Size: 1814 4086
Acc65I G'GTAC_C
Cuts at: 2309 2916 2309
Size: 607 5293
AclI AA'CG_TT
Cuts at: 5740 5740
Size: 5900
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1832 1855 3725 4157 4904 5172 1832
Size: 23 1870 432 747 268 2560
Fragments arranged by size:
2560 1870 747 432 268 23
AflIII A'CryG_T
Cuts at: 1499 3252 4356 5333 1499
Size: 1753 1104 977 2066
Fragments arranged by size:
2066 1753 1104 977
AgeI A'CCGG_T
Cuts at: 1910 4288 1910
Size: 2378 3522
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2416 2448 2416
Size: 32 5868
AlwNI CAG_nnn'CTG
Cuts at: 3205 4772 3205
Size: 1567 4333
ApaI G_GGCC'C
Cuts at: 976 1328 2105 2389 976
Size: 352 777 284 4487
Fragments arranged by size:
4487 777 352 284
ApaLI G'TGCA_C
Cuts at: 4670 4670
Size: 5900
ApoI r'AATT_y
Cuts at: 2904 5253 5509 2904
Size: 2349 256 3295
Fragments arranged by size:
3295 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5900
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5900
AvaI C'yCGr_G
Cuts at: 439 1559 2920 439
Size: 1120 1361 3419
Fragments arranged by size:
3419 1361 1120
BamHI G'GATC_C
Cuts at: 2925 2925
Size: 5900
BanI G'GyrC_C
Cuts at: 420 1741 2309 2916 3330 3578 3613 420
Size: 1321 568 607 414 248 35 2707
Fragments arranged by size:
2707 1321 607 568 414 248 35
BanII G_rGCy'C
Cuts at: 396 448 976 1100 1209 1328 1772 2105 2240
Size: 52 528 124 109 119 444 333 135
Cuts at: 2240 2348 2389 2914 3944 396
Size: 108 41 525 1030 2352
Fragments arranged by size:
2352 1030 528 525 444 333 135 124
119 109 108 52 41
BbeI G_GCGC'C
Cuts at: 3334 3582 3334
Size: 248 5652
BciVI GTATCCnnnnn_n'
Cuts at: 2095 3798 4559 5900 2095
Size: 1703 761 1341 2095
Fragments arranged by size:
2095 1703 1341 761
BclI T'GATC_A
Cuts at: 3420 3420
Size: 5900
BfrBI ATG'CAT
Cuts at: 2301 2602 2301
Size: 301 5599
BglI GCCn_nnn'nGGC
Cuts at: 151 1019 151
Size: 868 5032
BglII A'GATC_T
Cuts at: 2282 3415 5452 2282
Size: 1133 2037 2730
Fragments arranged by size:
2730 2037 1133
BlpI GC'TnA_GC
Cuts at: 2234 3012 2234
Size: 778 5122
Bme1580I G_kGCm'C
Cuts at: 976 1328 2105 2389 3525 3618 4674 976
Size: 352 777 284 1136 93 1056 2202
Fragments arranged by size:
2202 1136 1056 777 352 284 93
BmgBI CAC'GTC
Cuts at: 2277 2277
Size: 5900
BmrI ACTGGGnnnn_n'
Cuts at: 727 979 1775 2005 2236 2533 3112 3193 3528
Size: 252 796 230 231 297 579 81 335
Cuts at: 3528 5672 727
Size: 2144 955
Fragments arranged by size:
2144 955 796 579 335 297 252 231
230 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1915 4267 1915
Size: 2352 3548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 2086 2188 2350 2617 470
Size: 1616 102 162 267 3753
Fragments arranged by size:
3753 1616 267 162 102
Bpu10I CC'TnA_GC
Cuts at: 2177 2569 2177
Size: 392 5508
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3076 3955 4447 4745 3076
Size: 879 492 298 4231
Fragments arranged by size:
4231 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2050 5604 2050
Size: 3554 2346
BsaAI yAC'GTr
Cuts at: 1946 2892 3253 1946
Size: 946 361 4593
Fragments arranged by size:
4593 946 361
BsaBI GATnn'nnATC
Cuts at: 6 3414 5451 6
Size: 3408 2037 455
Fragments arranged by size:
3408 2037 455
BsaHI Gr'CG_yC
Cuts at: 3331 3579 4281 5777 3331
Size: 248 702 1496 3454
Fragments arranged by size:
3454 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1910 3089 3610 4288 4562 4709 5120 1910
Size: 1179 521 678 274 147 411 2690
Fragments arranged by size:
2690 1179 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1354 1384 2416 2446 1354
Size: 30 1032 30 4808
Fragments arranged by size:
4808 1032 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 270 1054 1264 1267 1954 2083 2860 270
Size: 784 210 3 687 129 777 3310
Fragments arranged by size:
3310 784 777 687 210 129 3
BseYI C'CCAG_C
Cuts at: 176 338 833 1443 2049 2400 3325 4083 4660
Size: 162 495 610 606 351 925 758 577
Cuts at: 4660 5156 176
Size: 496 920
Fragments arranged by size:
925 920 758 610 606 577 496 495
351 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1054 1478 1713 2299 2359 2776 1054
Size: 424 235 586 60 417 4178
Fragments arranged by size:
4178 586 424 417 235 60
BsiEI CG_ry'CG
Cuts at: 104 2150 3442 3488 4696 104
Size: 2046 1292 46 1208 1308
Fragments arranged by size:
2046 1308 1292 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2348 2914 3692 3882 4674 2348
Size: 566 778 190 792 3574
Fragments arranged by size:
3574 792 778 566 190
BsmI GAATG_Cn'
Cuts at: 2501 2604 2501
Size: 103 5797
BsmBI CGTCTCn'nnnn_
Cuts at: 2482 2671 2482
Size: 189 5711
Bsp1286I G_dGCh'C
Cuts at: 396 448 976 1100 1209 1328 1772 2105 2240
Size: 52 528 124 109 119 444 333 135
Cuts at: 2240 2348 2389 2914 3525 3618 3692 3882 3944
Size: 108 41 525 611 93 74 190 62
Cuts at: 3944 4674 396
Size: 730 1622
Fragments arranged by size:
1622 730 611 528 525 444 333 190
135 124 119 109 108 93 74 62
52 41
BspEI T'CCGG_A
Cuts at: 5120 5120
Size: 5900
BspHI T'CATG_A
Cuts at: 548 5076 548
Size: 4528 1372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2467 2950 3164 3466 3847 2467
Size: 483 214 302 381 4520
Fragments arranged by size:
4520 483 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1370 4192 4246 5730 5895 50
Size: 1320 2822 54 1484 165 55
Fragments arranged by size:
2822 1484 1320 165 55 54
BsrDI GCAATG_nn'
Cuts at: 269 3812 269
Size: 3543 2357
BsrFI r'CCGG_y
Cuts at: 1910 3898 4079 4288 1910
Size: 1988 181 209 3522
Fragments arranged by size:
3522 1988 209 181
BsrGI T'GTAC_A
Cuts at: 405 1673 405
Size: 1268 4632
BssSI C'ACGA_G
Cuts at: 646 693 4171 4529 646
Size: 47 3478 358 2017
Fragments arranged by size:
3478 2017 358 47
BstAPI GCAn_nnn'nTGC
Cuts at: 3277 3277
Size: 5900
BstBI TT'CG_AA
Cuts at: 110 4261 110
Size: 4151 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1620 2105 1620
Size: 485 5415
BstYI r'GATC_y
Cuts at: 1 728 1487 2282 2925 3392 3415 3750 3996
Size: 727 759 795 643 467 23 335 246
Cuts at: 3996 5008 5094 5106 5452 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 795 759 727 643 467 449 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1519 1519
Size: 5900
BtgI C'CryG_G
Cuts at: 125 291 885 2120 125
Size: 166 594 1235 3905
Fragments arranged by size:
3905 1235 594 166
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3850 4031 4194 3850
Size: 181 163 5556
Fragments arranged by size:
5556 181 163
BtsI GCAGTG_nn'
Cuts at: 371 1076 1804 3143 371
Size: 705 728 1339 3128
Fragments arranged by size:
3128 1339 728 705
ClaI AT'CG_AT
Cuts at: 1939 1939
Size: 5900
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1198 1233 1198
Size: 35 5865
DraI TTT'AAA
Cuts at: 705 2899 705
Size: 2194 3706
DraIII CAC_nnn'GTG
Cuts at: 2754 2754
Size: 5900
DrdI GACnn_nn'nnGTC
Cuts at: 3606 4464 3606
Size: 858 5042
EaeI y'GGCC_r
Cuts at: 288 294 428 915 939 1379 1610 2117 3485
Size: 6 134 487 24 440 231 507 1368
Cuts at: 3485 3659 4050 4077 5422 288
Size: 174 391 27 1345 766
Fragments arranged by size:
1368 1345 766 507 487 440 391 231
174 134 27 24 6
EagI C'GGCC_G
Cuts at: 3485 3485
Size: 5900
EarI CTCTTCn'nnn_
Cuts at: 387 770 1402 1478 2071 2545 2674 3923 4133
Size: 383 632 76 593 474 129 1249 210
Cuts at: 4133 387
Size: 2154
Fragments arranged by size:
2154 1249 632 593 474 383 210 129
76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2179 4108 4418 4564 5710 2179
Size: 1929 310 146 1146 2369
Fragments arranged by size:
2369 1929 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2346 2912 2346
Size: 566 5334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 1832 1855 2086 2188 2350 2617 3725 4157
Size: 1362 23 231 102 162 267 1108 432
Cuts at: 4157 4904 5172 470
Size: 747 268 1198
Fragments arranged by size:
1362 1198 1108 747 432 268 267 231
162 102 23
EcoNI CCTnn'n_nnAGG
Cuts at: 1343 2572 1343
Size: 1229 4671
EcoO109I rG'GnC_Cy
Cuts at: 153 264 973 1324 1325 1355 2101 2102 2385
Size: 111 709 351 1 30 746 1 283
Cuts at: 2385 2386 2578 3039 153
Size: 1 192 461 3014
Fragments arranged by size:
3014 746 709 461 351 283 192 111
30 1 1 1
EcoRI G'AATT_C
Cuts at: 2904 2904
Size: 5900
EcoRV GAT'ATC
Cuts at: 1540 1540
Size: 5900
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3919 3919
Size: 5900
FspI TGC'GCA
Cuts at: 3681 3681
Size: 5900
HaeII r_GCGC'y
Cuts at: 3334 3582 4604 5583 3334
Size: 248 1022 979 3651
Fragments arranged by size:
3651 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 745 777 2139 2171 2546 2562 2578 2594 3742
Size: 32 1362 32 375 16 16 16 1148
Cuts at: 3742 3772 3774 3804 745
Size: 30 2 30 2841
Fragments arranged by size:
2841 1362 1148 375 32 32 30 30
16 16 16 2
HincII GTy'rAC
Cuts at: 1725 2715 2939 1725
Size: 990 224 4686
Fragments arranged by size:
4686 990 224
HindIII A'AGCT_T
Cuts at: 1882 1882
Size: 5900
KasI G'GCGC_C
Cuts at: 3330 3578 3330
Size: 248 5652
KpnI G_GTAC'C
Cuts at: 2313 2920 2313
Size: 607 5293
MluI A'CGCG_T
Cuts at: 5333 5333
Size: 5900
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 788 1182 4571 4755 788
Size: 394 3389 184 1933
Fragments arranged by size:
3389 1933 394 184
MscI TGG'CCA
Cuts at: 430 941 1612 2119 3661 430
Size: 511 671 507 1542 2669
Fragments arranged by size:
2669 1542 671 511 507
MslI CAynn'nnrTG
Cuts at: 890 1504 1859 2878 3889 4016 890
Size: 614 355 1019 1011 127 2774
Fragments arranged by size:
2774 1019 1011 614 355 127
MspA1I CmG'CkG
Cuts at: 837 998 2184 2418 3011 3325 3685 4698 4943
Size: 161 1186 234 593 314 360 1013 245
Cuts at: 4943 837
Size: 1794
Fragments arranged by size:
1794 1186 1013 593 360 314 245 234
161
NaeI GCC'GGC
Cuts at: 4081 4081
Size: 5900
NarI GG'CG_CC
Cuts at: 3331 3579 3331
Size: 248 5652
NcoI C'CATG_G
Cuts at: 125 885 125
Size: 760 5140
NgoMIV G'CCGG_C
Cuts at: 4079 4079
Size: 5900
NsiI A_TGCA'T
Cuts at: 2303 2604 2303
Size: 301 5599
NspI r_CATG'y
Cuts at: 1503 2035 2305 2953 3453 4360 1503
Size: 532 270 648 500 907 3043
Fragments arranged by size:
3043 907 648 532 500 270
PasI CC'CwG_GG
Cuts at: 388 489 637 2105 388
Size: 101 148 1468 4183
Fragments arranged by size:
4183 1468 148 101
PciI A'CATG_T
Cuts at: 1499 4356 1499
Size: 2857 3043
PflMI CCAn_nnn'nTGG
Cuts at: 2400 2400
Size: 5900
PfoI T'CCnGG_A
Cuts at: 2087 2633 2087
Size: 546 5354
PmeI GTTT'AAAC
Cuts at: 2899 2899
Size: 5900
PmlI CAC'GTG
Cuts at: 1946 1946
Size: 5900
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2448 5039 5071 2416
Size: 32 2591 32 3245
Fragments arranged by size:
3245 2591 32 32
PpuMI rG'GwC_Cy
Cuts at: 264 1355 2578 264
Size: 1091 1223 3586
Fragments arranged by size:
3586 1223 1091
PsiI TTA'TAA
Cuts at: 1396 1396
Size: 5900
PspOMI G'GGCC_C
Cuts at: 972 1324 2101 2385 972
Size: 352 777 284 4487
Fragments arranged by size:
4487 777 352 284
PspXI vC'TCGA_Gb
Cuts at: 439 439
Size: 5900
PstI C_TGCA'G
Cuts at: 1169 1472 1591 2947 3632 1169
Size: 303 119 1356 685 3437
Fragments arranged by size:
3437 1356 685 303 119
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5900
PvuII CAG'CTG
Cuts at: 837 3325 3685 837
Size: 2488 360 3052
Fragments arranged by size:
3052 2488 360
RsrII CG'GwC_CG
Cuts at: 4095 4095
Size: 5900
SacI G_AGCT'C
Cuts at: 2348 2914 2348
Size: 566 5334
SalI G'TCGA_C
Cuts at: 2937 2937
Size: 5900
SanDI GG'GwC_CC
Cuts at: 2578 2578
Size: 5900
SapI GCTCTTCn'nnn_
Cuts at: 387 770 3923 4133 387
Size: 383 3153 210 2154
Fragments arranged by size:
3153 2154 383 210
SbfI CC_TGCA'GG
Cuts at: 2947 2947
Size: 5900
ScaI AGT'ACT
Cuts at: 1764 5417 1764
Size: 3653 2247
SfcI C'TryA_G
Cuts at: 33 1165 1468 1587 2943 3628 4621 4812 33
Size: 1132 303 119 1356 685 993 191 1121
Fragments arranged by size:
1356 1132 1121 993 685 303 191 119
SfoI GGC'GCC
Cuts at: 3332 3580 3332
Size: 248 5652
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5900
SmaI CCC'GGG
Cuts at: 2922 2922
Size: 5900
SmlI C'TyrA_G
Cuts at: 439 3055 3970 4462 4724 439
Size: 2616 915 492 262 1615
Fragments arranged by size:
2616 1615 915 492 262
SnaBI TAC'GTA
Cuts at: 2892 2892
Size: 5900
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5900
SphI G_CATG'C
Cuts at: 2035 2305 2953 3453 2035
Size: 270 648 500 4482
Fragments arranged by size:
4482 648 500 270
SspI AAT'ATT
Cuts at: 2523 2853 2523
Size: 330 5570
StuI AGG'CCT
Cuts at: 2785 2785
Size: 5900
StyI C'CwwG_G
Cuts at: 125 885 1376 2372 3034 3388 125
Size: 760 491 996 662 354 2637
Fragments arranged by size:
2637 996 760 662 491 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 425 3483 3616 4075 4284 425
Size: 3058 133 459 209 2041
Fragments arranged by size:
3058 2041 459 209 133
TatI w'GTAC_w
Cuts at: 405 1673 1762 2678 5415 405
Size: 1268 89 916 2737 890
Fragments arranged by size:
2737 1268 916 890 89
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1354 1386 5417 5449 1354
Size: 32 4031 32 1805
Fragments arranged by size:
4031 1805 32 32
Tth111I GACn'n_nGTC
Cuts at: 3697 3697
Size: 5900
XbaI T'CTAG_A
Cuts at: 2931 2931
Size: 5900
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2641 3395 2641
Size: 754 5146
XhoI C'TCGA_G
Cuts at: 439 439
Size: 5900
XmaI C'CCGG_G
Cuts at: 2920 2920
Size: 5900
XmnI GAAnn'nnTTC
Cuts at: 212 620 779 212
Size: 408 159 5333
Fragments arranged by size:
5333 408 159
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BbsI BbvCI BcgI BmtI BsiWI BssHII BstEII
BstZ17I FseI FspAI HpaI MfeI NdeI NheI NotI
NruI PacI PshAI PsrI SacII SexAI SfiI SgrAI
SrfI SwaI ZraI