Restriction map for
pF1KSDA0752
(Circular) (Six-base) MAPSORT of: pF1KSDA0752.seq Check: 5612 from: 1 to: 4069
pF1KSDA0752 4069 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 720 822 930 720
Size: 102 108 3859
Fragments arranged by size:
3859 108 102
AccI GT'mk_AC
Cuts at: 1105 1105
Size: 4069
Acc65I G'GTAC_C
Cuts at: 1083 1083
Size: 4069
AclI AA'CG_TT
Cuts at: 3909 3909
Size: 4069
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 562 1892 2324 3073 3341 416
Size: 146 1330 432 749 268 1144
Fragments arranged by size:
1330 1144 749 432 268 146
AflIII A'CryG_T
Cuts at: 1419 2525 3502 1419
Size: 1106 977 1986
Fragments arranged by size:
1986 1106 977
AgeI A'CCGG_T
Cuts at: 2457 2457
Size: 4069
AlwNI CAG_nnn'CTG
Cuts at: 328 502 585 700 802 1372 2941 328
Size: 174 83 115 102 570 1569 1456
Fragments arranged by size:
1569 1456 570 174 115 102 83
ApaLI G'TGCA_C
Cuts at: 2839 2839
Size: 4069
ApoI r'AATT_y
Cuts at: 1071 3422 3678 1071
Size: 2351 256 1462
Fragments arranged by size:
2351 1462 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4069
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4069
AvaI C'yCGr_G
Cuts at: 298 1087 298
Size: 789 3280
BamHI G'GATC_C
Cuts at: 1092 1092
Size: 4069
BanI G'GyrC_C
Cuts at: 442 1083 1497 1745 1780 442
Size: 641 414 248 35 2731
Fragments arranged by size:
2731 641 414 248 35
BanII G_rGCy'C
Cuts at: 191 1081 2111 191
Size: 890 1030 2149
Fragments arranged by size:
2149 1030 890
BbeI G_GCGC'C
Cuts at: 1501 1749 1501
Size: 248 3821
BbsI GAAGACnn'nnnn_
Cuts at: 238 473 634 671 773 872 238
Size: 235 161 37 102 99 3435
Fragments arranged by size:
3435 235 161 102 99 37
BciVI GTATCCnnnnn_n'
Cuts at: 1965 2728 4069 1965
Size: 763 1341 1965
Fragments arranged by size:
1965 1341 763
BclI T'GATC_A
Cuts at: 563 1587 563
Size: 1024 3045
BglI GCCn_nnn'nGGC
Cuts at: 748 850 748
Size: 102 3967
BglII A'GATC_T
Cuts at: 1582 3621 1582
Size: 2039 2030
BlpI GC'TnA_GC
Cuts at: 1179 1179
Size: 4069
Bme1580I G_kGCm'C
Cuts at: 1692 1785 2843 1692
Size: 93 1058 2918
Fragments arranged by size:
2918 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1279 1360 1695 3841 1279
Size: 81 335 2146 1507
Fragments arranged by size:
2146 1507 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 324 2434 324
Size: 2110 1959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 691 793 493
Size: 198 102 3769
Fragments arranged by size:
3769 198 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1243 2122 2616 2914 1243
Size: 879 494 298 2398
Fragments arranged by size:
2398 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 3773 3773
Size: 4069
BsaAI yAC'GTr
Cuts at: 1059 1420 1059
Size: 361 3708
BsaBI GATnn'nnATC
Cuts at: 6 1581 3620 6
Size: 1575 2039 455
Fragments arranged by size:
2039 1575 455
BsaHI Gr'CG_yC
Cuts at: 1498 1746 2450 3946 1498
Size: 248 704 1496 1621
Fragments arranged by size:
1621 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1256 1777 2457 2731 2878 3289 1256
Size: 521 680 274 147 411 2036
Fragments arranged by size:
2036 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 173 203 256 286 173
Size: 30 53 30 3956
Fragments arranged by size:
3956 53 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 385 406 490 619 622 634 688 790 385
Size: 21 84 129 3 12 54 102 3664
Fragments arranged by size:
3664 129 102 84 54 21 12 3
BseYI C'CCAG_C
Cuts at: 738 840 1000 1492 2250 2829 3325 738
Size: 102 160 492 758 579 496 1482
Fragments arranged by size:
1482 758 579 496 492 160 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 902
Size: 4069
BsiEI CG_ry'CG
Cuts at: 104 1609 1655 2865 104
Size: 1505 46 1210 1308
Fragments arranged by size:
1505 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 191 1081 1859 2049 2843 191
Size: 890 778 190 794 1417
Fragments arranged by size:
1417 890 794 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 307 307
Size: 4069
Bsp1286I G_dGCh'C
Cuts at: 191 1081 1692 1785 1859 2049 2111 2843 191
Size: 890 611 93 74 190 62 732 1417
Fragments arranged by size:
1417 890 732 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3289 3289
Size: 4069
BspHI T'CATG_A
Cuts at: 3245 3245
Size: 4069
BspMI ACCTGCnnnn'nnnn_
Cuts at: 720 822 930 1117 1331 1633 2014 720
Size: 102 108 187 214 302 381 2775
Fragments arranged by size:
2775 381 302 214 187 108 102
BsrBI CCG'CTC
Cuts at: 50 2359 2413 3899 4064 50
Size: 2309 54 1486 165 55
Fragments arranged by size:
2309 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1979 1979
Size: 4069
BsrFI r'CCGG_y
Cuts at: 1013 2065 2246 2457 1013
Size: 1052 181 211 2625
Fragments arranged by size:
2625 1052 211 181
BssSI C'ACGA_G
Cuts at: 2338 2698 2338
Size: 360 3709
BstAPI GCAn_nnn'nTGC
Cuts at: 1444 1444
Size: 4069
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4069
BstEII G'GTnAC_C
Cuts at: 457 655 457
Size: 198 3871
BstYI r'GATC_y
Cuts at: 1 1092 1559 1582 1917 2163 3177 3263 3275
Size: 1091 467 23 335 246 1014 86 12
Cuts at: 3275 3621 1
Size: 346 449
Fragments arranged by size:
1091 1014 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 4069
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 241 2017 2198 2361 241
Size: 1776 181 163 1949
Fragments arranged by size:
1949 1776 181 163
BtsI GCAGTG_nn'
Cuts at: 1310 1310
Size: 4069
DraI TTT'AAA
Cuts at: 1066 1066
Size: 4069
DraIII CAC_nnn'GTG
Cuts at: 256 652 256
Size: 396 3673
DrdI GACnn_nn'nnGTC
Cuts at: 1773 2633 1773
Size: 860 3209
EaeI y'GGCC_r
Cuts at: 551 1652 1826 2217 2244 3591 551
Size: 1101 174 391 27 1347 1029
Fragments arranged by size:
1347 1101 1029 391 174 27
EagI C'GGCC_G
Cuts at: 1652 1652
Size: 4069
EarI CTCTTCn'nnn_
Cuts at: 950 2090 2300 950
Size: 1140 210 2719
Fragments arranged by size:
2719 1140 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2275 2587 2733 3879 2275
Size: 312 146 1146 2465
Fragments arranged by size:
2465 1146 312 146
EcoICRI GAG'CTC
Cuts at: 189 1079 189
Size: 890 3179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 493 562 691 793 1892 2324 3073 3341
Size: 77 69 129 102 1099 432 749 268
Cuts at: 3341 416
Size: 1144
Fragments arranged by size:
1144 1099 749 432 268 129 102 77
69
EcoO109I rG'GnC_Cy
Cuts at: 537 612 1206 537
Size: 75 594 3400
Fragments arranged by size:
3400 594 75
EcoRI G'AATT_C
Cuts at: 1071 1071
Size: 4069
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 255 651 2086 255
Size: 396 1435 2238
Fragments arranged by size:
2238 1435 396
FspI TGC'GCA
Cuts at: 1848 1848
Size: 4069
HaeII r_GCGC'y
Cuts at: 1501 1749 2773 3752 1501
Size: 248 1024 979 1818
Fragments arranged by size:
1818 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 256 288 1909 1939 1941 1971 256
Size: 32 1621 30 2 30 2354
Fragments arranged by size:
2354 1621 32 30 30 2
HincII GTy'rAC
Cuts at: 1106 1106
Size: 4069
KasI G'GCGC_C
Cuts at: 1497 1745 1497
Size: 248 3821
KpnI G_GTAC'C
Cuts at: 1087 1087
Size: 4069
MfeI C'AATT_G
Cuts at: 859 859
Size: 4069
MluI A'CGCG_T
Cuts at: 3502 3502
Size: 4069
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2740 2924 2740
Size: 184 3885
MscI TGG'CCA
Cuts at: 1828 1828
Size: 4069
MslI CAynn'nnrTG
Cuts at: 157 2056 2183 157
Size: 1899 127 2043
Fragments arranged by size:
2043 1899 127
MspA1I CmG'CkG
Cuts at: 1178 1492 1852 2867 3112 1178
Size: 314 360 1015 245 2135
Fragments arranged by size:
2135 1015 360 314 245
NaeI GCC'GGC
Cuts at: 2248 2248
Size: 4069
NarI GG'CG_CC
Cuts at: 1498 1746 1498
Size: 248 3821
NcoI C'CATG_G
Cuts at: 125 125
Size: 4069
NgoMIV G'CCGG_C
Cuts at: 2246 2246
Size: 4069
NruI TCG'CGA
Cuts at: 2430 2430
Size: 4069
NspI r_CATG'y
Cuts at: 682 784 1120 1620 2529 682
Size: 102 336 500 909 2222
Fragments arranged by size:
2222 909 500 336 102
PciI A'CATG_T
Cuts at: 2525 2525
Size: 4069
PflMI CCAn_nnn'nTGG
Cuts at: 652 652
Size: 4069
PmeI GTTT'AAAC
Cuts at: 1066 1066
Size: 4069
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3208 3240 3208
Size: 32 4037
PpuMI rG'GwC_Cy
Cuts at: 537 612 537
Size: 75 3994
PstI C_TGCA'G
Cuts at: 927 1114 1799 927
Size: 187 685 3197
Fragments arranged by size:
3197 685 187
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4069
PvuII CAG'CTG
Cuts at: 1492 1852 1492
Size: 360 3709
RsrII CG'GwC_CG
Cuts at: 2262 2262
Size: 4069
SacI G_AGCT'C
Cuts at: 191 1081 191
Size: 890 3179
SalI G'TCGA_C
Cuts at: 1104 1104
Size: 4069
SanDI GG'GwC_CC
Cuts at: 537 537
Size: 4069
SapI GCTCTTCn'nnn_
Cuts at: 2090 2300 2090
Size: 210 3859
SbfI CC_TGCA'GG
Cuts at: 1114 1114
Size: 4069
ScaI AGT'ACT
Cuts at: 3586 3586
Size: 4069
SfcI C'TryA_G
Cuts at: 33 923 1110 1795 2790 2981 33
Size: 890 187 685 995 191 1121
Fragments arranged by size:
1121 995 890 685 191 187
SfoI GGC'GCC
Cuts at: 1499 1747 1499
Size: 248 3821
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4069
SmaI CCC'GGG
Cuts at: 1089 1089
Size: 4069
SmlI C'TyrA_G
Cuts at: 1222 2137 2631 2893 1222
Size: 915 494 262 2398
Fragments arranged by size:
2398 915 494 262
SnaBI TAC'GTA
Cuts at: 1059 1059
Size: 4069
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4069
SphI G_CATG'C
Cuts at: 1120 1620 1120
Size: 500 3569
StyI C'CwwG_G
Cuts at: 125 239 275 635 1201 1555 125
Size: 114 36 360 566 354 2639
Fragments arranged by size:
2639 566 360 354 114 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 460 1650 1783 2242 2453 460
Size: 1190 133 459 211 2076
Fragments arranged by size:
2076 1190 459 211 133
TatI w'GTAC_w
Cuts at: 573 3584 573
Size: 3011 1058
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3586 3618 3586
Size: 32 4037
Tth111I GACn'n_nGTC
Cuts at: 1864 1864
Size: 4069
XbaI T'CTAG_A
Cuts at: 366 1098 366
Size: 732 3337
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 459 1562 459
Size: 1103 2966
XmaI C'CCGG_G
Cuts at: 1087 1087
Size: 4069
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SrfI SspI StuI SwaI XhoI XmnI ZraI