Restriction map for
pF1KSDA0753
(Circular) (Six-base) MAPSORT of: pF1KSDA0753.seq Check: 2467 from: 1 to: 6040
pF1KSDA0753 6040 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 644 1410 1695 3076 644
Size: 766 285 1381 3608
Fragments arranged by size:
3608 1381 766 285
Acc65I G'GTAC_C
Cuts at: 3054 3054
Size: 6040
AclI AA'CG_TT
Cuts at: 5880 5880
Size: 6040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 331 703 2126 2947 3863 4295 5044 5312
Size: 138 372 1423 821 916 432 749 268
Cuts at: 5312 193
Size: 921
Fragments arranged by size:
1423 921 916 821 749 432 372 268
138
AflIII A'CryG_T
Cuts at: 3390 4496 5473 3390
Size: 1106 977 3957
Fragments arranged by size:
3957 1106 977
AgeI A'CCGG_T
Cuts at: 4428 4428
Size: 6040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1451 1451
Size: 6040
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 869 901 869
Size: 32 6008
AlwNI CAG_nnn'CTG
Cuts at: 1933 2992 3343 4912 1933
Size: 1059 351 1569 3061
Fragments arranged by size:
3061 1569 1059 351
ApaLI G'TGCA_C
Cuts at: 4810 4810
Size: 6040
ApoI r'AATT_y
Cuts at: 690 1292 3007 3042 5393 5649 690
Size: 602 1715 35 2351 256 1081
Fragments arranged by size:
2351 1715 1081 602 256 35
AseI AT'TA_AT
Cuts at: 20 20
Size: 6040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6040
AvaI C'yCGr_G
Cuts at: 3058 3058
Size: 6040
AvrII C'CTAG_G
Cuts at: 169 169
Size: 6040
BamHI G'GATC_C
Cuts at: 864 2643 3063 864
Size: 1779 420 3841
Fragments arranged by size:
3841 1779 420
BanI G'GyrC_C
Cuts at: 634 1653 1788 3054 3468 3716 3751 634
Size: 1019 135 1266 414 248 35 2923
Fragments arranged by size:
2923 1266 1019 414 248 135 35
BanII G_rGCy'C
Cuts at: 2099 2372 2787 3052 4082 2099
Size: 273 415 265 1030 4057
Fragments arranged by size:
4057 1030 415 273 265
BbeI G_GCGC'C
Cuts at: 1657 3472 3720 1657
Size: 1815 248 3977
Fragments arranged by size:
3977 1815 248
BbsI GAAGACnn'nnnn_
Cuts at: 462 2632 462
Size: 2170 3870
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 263 297 895 929 2836 2870 2965 2999 263
Size: 34 598 34 1907 34 95 34 3304
Fragments arranged by size:
3304 1907 598 95 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 823 1790 3936 4699 6040 823
Size: 967 2146 763 1341 823
Fragments arranged by size:
2146 1341 967 823 763
BclI T'GATC_A
Cuts at: 3558 3558
Size: 6040
BglI GCCn_nnn'nGGC
Cuts at: 1060 1060
Size: 6040
BglII A'GATC_T
Cuts at: 675 2393 2445 3553 5592 675
Size: 1718 52 1108 2039 1123
Fragments arranged by size:
2039 1718 1123 1108 52
BlpI GC'TnA_GC
Cuts at: 3150 3150
Size: 6040
Bme1580I G_kGCm'C
Cuts at: 3663 3756 4814 3663
Size: 93 1058 4889
Fragments arranged by size:
4889 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1141 3250 3331 3666 5812 1141
Size: 2109 81 335 2146 1369
Fragments arranged by size:
2146 2109 1369 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2806 4405 2806
Size: 1599 4441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 609 775 925 1876 2470 609
Size: 166 150 951 594 4179
Fragments arranged by size:
4179 951 594 166 150
Bpu10I CC'TnA_GC
Cuts at: 2412 2412
Size: 6040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1892 3214 4093 4587 4885 1892
Size: 1322 879 494 298 3047
Fragments arranged by size:
3047 1322 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1465 5744 1465
Size: 4279 1761
BsaAI yAC'GTr
Cuts at: 3030 3391 3030
Size: 361 5679
BsaBI GATnn'nnATC
Cuts at: 6 1445 3552 5591 6
Size: 1439 2107 2039 455
Fragments arranged by size:
2107 2039 1439 455
BsaHI Gr'CG_yC
Cuts at: 1654 3469 3717 4421 5917 1654
Size: 1815 248 704 1496 1777
Fragments arranged by size:
1815 1777 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1467 3227 3748 4428 4702 4849 5260 1467
Size: 1760 521 680 274 147 411 2247
Fragments arranged by size:
2247 1760 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 204 1064 1380 2182 2594 204
Size: 860 316 802 412 3650
Fragments arranged by size:
3650 860 802 412 316
BseYI C'CCAG_C
Cuts at: 2282 3463 4221 4800 5296 2282
Size: 1181 758 579 496 3026
Fragments arranged by size:
3026 1181 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1747 2273 1747
Size: 526 5514
BsiEI CG_ry'CG
Cuts at: 104 3580 3626 4836 104
Size: 3476 46 1210 1308
Fragments arranged by size:
3476 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 2099 2372 2787 3052 3830 4020 4814 2099
Size: 273 415 265 778 190 794 3325
Fragments arranged by size:
3325 794 778 415 273 265 190
BsmI GAATG_Cn'
Cuts at: 932 932
Size: 6040
BsmBI CGTCTCn'nnnn_
Cuts at: 1832 1832
Size: 6040
Bsp1286I G_dGCh'C
Cuts at: 2099 2372 2787 3052 3663 3756 3830 4020 4082
Size: 273 415 265 611 93 74 190 62
Cuts at: 4082 4814 2099
Size: 732 3325
Fragments arranged by size:
3325 732 611 415 273 265 190 93
74 62
BspEI T'CCGG_A
Cuts at: 1467 5260 1467
Size: 3793 2247
BspHI T'CATG_A
Cuts at: 2456 2868 5216 2456
Size: 412 2348 3280
Fragments arranged by size:
3280 2348 412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 939 3088 3302 3604 3985 939
Size: 2149 214 302 381 2994
Fragments arranged by size:
2994 2149 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4330 4384 5870 6035 50
Size: 4280 54 1486 165 55
Fragments arranged by size:
4280 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1028 3950 1028
Size: 2922 3118
BsrFI r'CCGG_y
Cuts at: 1763 4036 4217 4428 1763
Size: 2273 181 211 3375
Fragments arranged by size:
3375 2273 211 181
BssSI C'ACGA_G
Cuts at: 4309 4669 4309
Size: 360 5680
BstAPI GCAn_nnn'nTGC
Cuts at: 3415 3415
Size: 6040
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6040
BstEII G'GTnAC_C
Cuts at: 739 739
Size: 6040
BstXI CCAn_nnnn'nTGG
Cuts at: 184 184
Size: 6040
BstYI r'GATC_y
Cuts at: 1 675 864 1902 2393 2445 2643 3063 3530
Size: 674 189 1038 491 52 198 420 467
Cuts at: 3530 3553 3888 4134 5148 5234 5246 5592 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
1038 1014 674 491 467 449 420 346
335 246 198 189 86 52 23 12
BstZ17I GTA'TAC
Cuts at: 645 645
Size: 6040
BtgI C'CryG_G
Cuts at: 125 1580 1792 2893 125
Size: 1455 212 1101 3272
Fragments arranged by size:
3272 1455 1101 212
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3988 4169 4332 3988
Size: 181 163 5696
Fragments arranged by size:
5696 181 163
BtsI GCAGTG_nn'
Cuts at: 3281 3281
Size: 6040
DraI TTT'AAA
Cuts at: 3037 3037
Size: 6040
DraIII CAC_nnn'GTG
Cuts at: 555 555
Size: 6040
DrdI GACnn_nn'nnGTC
Cuts at: 3744 4604 3744
Size: 860 5180
EaeI y'GGCC_r
Cuts at: 1339 3623 3797 4188 4215 5562 1339
Size: 2284 174 391 27 1347 1817
Fragments arranged by size:
2284 1817 1347 391 174 27
EagI C'GGCC_G
Cuts at: 3623 3623
Size: 6040
EarI CTCTTCn'nnn_
Cuts at: 435 821 1997 2468 2747 2915 4061 4271 435
Size: 386 1176 471 279 168 1146 210 2204
Fragments arranged by size:
2204 1176 1146 471 386 279 210 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4246 4558 4704 5850 4246
Size: 312 146 1146 4436
Fragments arranged by size:
4436 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2097 2370 2785 3050 2097
Size: 273 415 265 5087
Fragments arranged by size:
5087 415 273 265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 331 609 703 775 925 1876 2126 2470
Size: 138 278 94 72 150 951 250 344
Cuts at: 2470 2947 3863 4295 5044 5312 193
Size: 477 916 432 749 268 921
Fragments arranged by size:
951 921 916 749 477 432 344 278
268 250 150 138 94 72
EcoO109I rG'GnC_Cy
Cuts at: 173 1370 2672 2736 3177 173
Size: 1197 1302 64 441 3036
Fragments arranged by size:
3036 1302 1197 441 64
EcoRI G'AATT_C
Cuts at: 3007 3042 3007
Size: 35 6005
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 430 664 1430 2214 4057 430
Size: 234 766 784 1843 2413
Fragments arranged by size:
2413 1843 784 766 234
FspI TGC'GCA
Cuts at: 3819 3819
Size: 6040
HaeII r_GCGC'y
Cuts at: 1657 3472 3720 4744 5723 1657
Size: 1815 248 1024 979 1974
Fragments arranged by size:
1974 1815 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1295 1327 2245 2277 2302 2334 2619 2651 2704
Size: 32 918 32 25 32 285 32 53
Cuts at: 2704 2736 2850 2882 3880 3910 3912 3942 1295
Size: 32 114 32 998 30 2 30 3393
Fragments arranged by size:
3393 998 918 285 114 53 32 32
32 32 32 32 30 30 25 2
HincII GTy'rAC
Cuts at: 1411 3077 1411
Size: 1666 4374
HindIII A'AGCT_T
Cuts at: 857 1557 2910 857
Size: 700 1353 3987
Fragments arranged by size:
3987 1353 700
KasI G'GCGC_C
Cuts at: 1653 3468 3716 1653
Size: 1815 248 3977
Fragments arranged by size:
3977 1815 248
KpnI G_GTAC'C
Cuts at: 3058 3058
Size: 6040
MfeI C'AATT_G
Cuts at: 2128 2128
Size: 6040
MluI A'CGCG_T
Cuts at: 5473 5473
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2148 2345 4711 4895 2148
Size: 197 2366 184 3293
Fragments arranged by size:
3293 2366 197 184
MscI TGG'CCA
Cuts at: 1341 3799 1341
Size: 2458 3582
MslI CAynn'nnrTG
Cuts at: 4027 4154 4027
Size: 127 5913
MspA1I CmG'CkG
Cuts at: 225 2305 3149 3463 3823 4838 5083 225
Size: 2080 844 314 360 1015 245 1182
Fragments arranged by size:
2080 1182 1015 844 360 314 245
NaeI GCC'GGC
Cuts at: 4219 4219
Size: 6040
NarI GG'CG_CC
Cuts at: 1654 3469 3717 1654
Size: 1815 248 3977
Fragments arranged by size:
3977 1815 248
NcoI C'CATG_G
Cuts at: 125 1792 125
Size: 1667 4373
NdeI CA'TA_TG
Cuts at: 2526 2526
Size: 6040
NgoMIV G'CCGG_C
Cuts at: 4217 4217
Size: 6040
NruI TCG'CGA
Cuts at: 4401 4401
Size: 6040
NspI r_CATG'y
Cuts at: 292 2034 3091 3591 4500 292
Size: 1742 1057 500 909 1832
Fragments arranged by size:
1832 1742 1057 909 500
PciI A'CATG_T
Cuts at: 4496 4496
Size: 6040
PflMI CCAn_nnn'nTGG
Cuts at: 555 555
Size: 6040
PfoI T'CCnGG_A
Cuts at: 711 962 1244 711
Size: 251 282 5507
Fragments arranged by size:
5507 282 251
PmeI GTTT'AAAC
Cuts at: 3037 3037
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 473 505 5179 5211 473
Size: 32 4674 32 1302
Fragments arranged by size:
4674 1302 32 32
PpuMI rG'GwC_Cy
Cuts at: 173 173
Size: 6040
PstI C_TGCA'G
Cuts at: 230 3085 3770 230
Size: 2855 685 2500
Fragments arranged by size:
2855 2500 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6040
PvuII CAG'CTG
Cuts at: 225 2305 3463 3823 225
Size: 2080 1158 360 2442
Fragments arranged by size:
2442 2080 1158 360
RsrII CG'GwC_CG
Cuts at: 4233 4233
Size: 6040
SacI G_AGCT'C
Cuts at: 2099 2372 2787 3052 2099
Size: 273 415 265 5087
Fragments arranged by size:
5087 415 273 265
SalI G'TCGA_C
Cuts at: 1409 3075 1409
Size: 1666 4374
SapI GCTCTTCn'nnn_
Cuts at: 2915 4061 4271 2915
Size: 1146 210 4684
Fragments arranged by size:
4684 1146 210
SbfI CC_TGCA'GG
Cuts at: 3085 3085
Size: 6040
ScaI AGT'ACT
Cuts at: 206 5557 206
Size: 5351 689
SfcI C'TryA_G
Cuts at: 33 226 1418 2228 2832 2876 3081 3766 4761
Size: 193 1192 810 604 44 205 685 995
Cuts at: 4761 4952 33
Size: 191 1121
Fragments arranged by size:
1192 1121 995 810 685 604 205 193
191 44
SfoI GGC'GCC
Cuts at: 1655 3470 3718 1655
Size: 1815 248 3977
Fragments arranged by size:
3977 1815 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6040
SmaI CCC'GGG
Cuts at: 3060 3060
Size: 6040
SmlI C'TyrA_G
Cuts at: 1907 3193 4108 4602 4864 1907
Size: 1286 915 494 262 3083
Fragments arranged by size:
3083 1286 915 494 262
SnaBI TAC'GTA
Cuts at: 3030 3030
Size: 6040
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6040
SphI G_CATG'C
Cuts at: 2034 3091 3591 2034
Size: 1057 500 4483
Fragments arranged by size:
4483 1057 500
SspI AAT'ATT
Cuts at: 2241 2241
Size: 6040
StuI AGG'CCT
Cuts at: 1327 1327
Size: 6040
StyI C'CwwG_G
Cuts at: 125 169 1328 1792 2015 3172 3526 125
Size: 44 1159 464 223 1157 354 2639
Fragments arranged by size:
2639 1159 1157 464 354 223 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 714 718 1109 2161 2932 3621 3754 4213 4424
Size: 4 391 1052 771 689 133 459 211
Cuts at: 4424 714
Size: 2330
Fragments arranged by size:
2330 1052 771 689 459 391 211 133
4
TatI w'GTAC_w
Cuts at: 204 1345 5555 204
Size: 1141 4210 689
Fragments arranged by size:
4210 1141 689
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 592 624 693 725 5557 5589 592
Size: 32 69 32 4832 32 1043
Fragments arranged by size:
4832 1043 69 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3835 3835
Size: 6040
XbaI T'CTAG_A
Cuts at: 1542 2071 3069 1542
Size: 529 998 4513
Fragments arranged by size:
4513 998 529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3533 3533
Size: 6040
XmaI C'CCGG_G
Cuts at: 3058 3058
Size: 6040
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NruI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
BaeI BbvCI BfrBI BmgBI BmtI BsaXI BsiWI BsrGI
BssHII Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI NheI NotI NsiI PacI PasI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI SwaI XhoI XmnI ZraI