Restriction map for
pF1KSDA0763
(Circular) (Six-base) MAPSORT of: pF1KSDA0763.seq Check: 7436 from: 1 to: 5662
pF1KSDA0763 5662 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 2698 2698
Size: 5662
Acc65I G'GTAC_C
Cuts at: 2676 2676
Size: 5662
AclI AA'CG_TT
Cuts at: 5502 5502
Size: 5662
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3485 3917 4666 4934 3485
Size: 432 749 268 4213
Fragments arranged by size:
4213 749 432 268
AfeI AGC'GCT
Cuts at: 226 1660 226
Size: 1434 4228
AflIII A'CryG_T
Cuts at: 1754 3012 4118 5095 1754
Size: 1258 1106 977 2321
Fragments arranged by size:
2321 1258 1106 977
AgeI A'CCGG_T
Cuts at: 4050 4050
Size: 5662
AhdI GACnn_n'nnGTC
Cuts at: 732 1914 732
Size: 1182 4480
AleI CACnn'nnGTG
Cuts at: 1604 1604
Size: 5662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2551 2551
Size: 5662
AlwNI CAG_nnn'CTG
Cuts at: 873 2199 2965 4534 873
Size: 1326 766 1569 2001
Fragments arranged by size:
2001 1569 1326 766
ApaI G_GGCC'C
Cuts at: 588 795 588
Size: 207 5455
ApaLI G'TGCA_C
Cuts at: 319 4432 319
Size: 4113 1549
ApoI r'AATT_y
Cuts at: 1588 2314 2605 2664 5015 5271 1588
Size: 726 291 59 2351 256 1979
Fragments arranged by size:
2351 1979 726 291 256 59
AseI AT'TA_AT
Cuts at: 20 20
Size: 5662
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5662
AvaI C'yCGr_G
Cuts at: 587 1052 1777 1978 2386 2680 587
Size: 465 725 201 408 294 3569
Fragments arranged by size:
3569 725 465 408 294 201
BamHI G'GATC_C
Cuts at: 1877 1966 2685 1877
Size: 89 719 4854
Fragments arranged by size:
4854 719 89
BanI G'GyrC_C
Cuts at: 149 561 1033 2676 3090 3338 3373 149
Size: 412 472 1643 414 248 35 2438
Fragments arranged by size:
2438 1643 472 414 412 248 35
BanII G_rGCy'C
Cuts at: 384 419 588 795 1985 2387 2674 3704 384
Size: 35 169 207 1190 402 287 1030 2342
Fragments arranged by size:
2342 1190 1030 402 287 207 169 35
BbeI G_GCGC'C
Cuts at: 153 1037 3094 3342 153
Size: 884 2057 248 2473
Fragments arranged by size:
2473 2057 884 248
BbvCI CC'TCA_GC
Cuts at: 409 1465 2524 409
Size: 1056 1059 3547
Fragments arranged by size:
3547 1059 1056
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2473 2507 2473
Size: 34 5628
BciVI GTATCCnnnnn_n'
Cuts at: 3558 4321 5662 3558
Size: 763 1341 3558
Fragments arranged by size:
3558 1341 763
BclI T'GATC_A
Cuts at: 3180 3180
Size: 5662
BglI GCCn_nnn'nGGC
Cuts at: 1086 1086
Size: 5662
BglII A'GATC_T
Cuts at: 2126 3175 5214 2126
Size: 1049 2039 2574
Fragments arranged by size:
2574 2039 1049
BlpI GC'TnA_GC
Cuts at: 1119 1258 2772 1119
Size: 139 1514 4009
Fragments arranged by size:
4009 1514 139
Bme1580I G_kGCm'C
Cuts at: 323 588 795 1418 3285 3378 4436 323
Size: 265 207 623 1867 93 1058 1549
Fragments arranged by size:
1867 1549 1058 623 265 207 93
BmgBI CAC'GTC
Cuts at: 1187 1524 1187
Size: 337 5325
BmrI ACTGGGnnnn_n'
Cuts at: 798 2418 2872 2953 3288 5434 798
Size: 1620 454 81 335 2146 1026
Fragments arranged by size:
2146 1620 1026 454 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4027 4027
Size: 5662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 496 874 429
Size: 67 378 5217
Fragments arranged by size:
5217 378 67
Bpu10I CC'TnA_GC
Cuts at: 409 1465 2524 409
Size: 1056 1059 3547
Fragments arranged by size:
3547 1059 1056
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 946 2285 2494 2836 3715 4209 4507 412
Size: 534 1339 209 342 879 494 298 1567
Fragments arranged by size:
1567 1339 879 534 494 342 298 209
BsaI GGTCTCn'nnnn_
Cuts at: 354 5366 354
Size: 5012 650
BsaAI yAC'GTr
Cuts at: 2652 3013 2652
Size: 361 5301
BsaBI GATnn'nnATC
Cuts at: 6 1864 3174 5213 6
Size: 1858 1310 2039 455
Fragments arranged by size:
2039 1858 1310 455
BsaHI Gr'CG_yC
Cuts at: 150 461 482 1034 2402 2568 3091 3339 4043
Size: 311 21 552 1368 166 523 248 704
Cuts at: 4043 5539 150
Size: 1496 273
Fragments arranged by size:
1496 1368 704 552 523 311 273 248
166 21
BsaWI w'CCGG_w
Cuts at: 1697 2306 2849 3370 4050 4324 4471 4882 1697
Size: 609 543 521 680 274 147 411 2477
Fragments arranged by size:
2477 680 609 543 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 352 371 382 401 679 709 940 970 1051
Size: 19 11 19 278 30 231 30 81
Cuts at: 1051 1081 1201 1231 352
Size: 30 120 30 4783
Fragments arranged by size:
4783 278 231 120 81 30 30 30
30 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 571 700 703 1072 571
Size: 129 3 369 5161
Fragments arranged by size:
5161 369 129 3
BseYI C'CCAG_C
Cuts at: 371 375 766 913 1295 2413 3085 3843 4422
Size: 4 391 147 382 1118 672 758 579
Cuts at: 4422 4918 371
Size: 496 1115
Fragments arranged by size:
1118 1115 758 672 579 496 391 382
147 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 598 1945 532
Size: 66 1347 4249
Fragments arranged by size:
4249 1347 66
BsiEI CG_ry'CG
Cuts at: 104 733 1964 3202 3248 4458 104
Size: 629 1231 1238 46 1210 1308
Fragments arranged by size:
1308 1238 1231 1210 629 46
BsiHKAI G_wGCw'C
Cuts at: 323 844 1529 1997 2387 2431 2647 2674 3452
Size: 521 685 468 390 44 216 27 778
Cuts at: 3452 3642 4436 323
Size: 190 794 1549
Fragments arranged by size:
1549 794 778 685 521 468 390 216
190 44 27
Bsp1286I G_dGCh'C
Cuts at: 323 384 419 588 795 844 1418 1529 1985
Size: 61 35 169 207 49 574 111 456
Cuts at: 1985 1997 2387 2431 2647 2674 3285 3378 3452
Size: 12 390 44 216 27 611 93 74
Cuts at: 3452 3642 3704 4436 323
Size: 190 62 732 1549
Fragments arranged by size:
1549 732 611 574 456 390 216 207
190 169 111 93 74 62 61 49
44 35 27 12
BspEI T'CCGG_A
Cuts at: 1697 4882 1697
Size: 3185 2477
BspHI T'CATG_A
Cuts at: 4838 4838
Size: 5662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 336 761 2184 2336 2710 2924 3226 3607 336
Size: 425 1423 152 374 214 302 381 2391
Fragments arranged by size:
2391 1423 425 381 374 302 214 152
BsrBI CCG'CTC
Cuts at: 50 858 944 951 1635 1782 3952 4006 5492
Size: 808 86 7 684 147 2170 54 1486
Cuts at: 5492 5657 50
Size: 165 55
Fragments arranged by size:
2170 1486 808 684 165 147 86 55
54 7
BsrDI GCAATG_nn'
Cuts at: 2339 2604 3572 2339
Size: 265 968 4429
Fragments arranged by size:
4429 968 265
BsrFI r'CCGG_y
Cuts at: 564 728 1127 1469 1499 2030 3658 3839 4050
Size: 164 399 342 30 531 1628 181 211
Cuts at: 4050 564
Size: 2176
Fragments arranged by size:
2176 1628 531 399 342 211 181 164
30
BsrGI T'GTAC_A
Cuts at: 674 1757 674
Size: 1083 4579
BssSI C'ACGA_G
Cuts at: 828 3931 4291 828
Size: 3103 360 2199
Fragments arranged by size:
3103 2199 360
BstAPI GCAn_nnn'nTGC
Cuts at: 873 2199 3037 873
Size: 1326 838 3498
Fragments arranged by size:
3498 1326 838
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5662
BstEII G'GTnAC_C
Cuts at: 156 403 2118 156
Size: 247 1715 3700
Fragments arranged by size:
3700 1715 247
BstXI CCAn_nnnn'nTGG
Cuts at: 788 1092 1569 788
Size: 304 477 4881
Fragments arranged by size:
4881 477 304
BstYI r'GATC_y
Cuts at: 1 1865 1877 1966 2126 2685 3152 3175 3510
Size: 1864 12 89 160 559 467 23 335
Cuts at: 3510 3756 4770 4856 4868 5214 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1864 1014 559 467 449 346 335 246
160 89 86 23 12 12
Bsu36I CC'TnA_GG
Cuts at: 1582 1582
Size: 5662
BtgI C'CryG_G
Cuts at: 1562 1562
Size: 5662
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3610 3791 3954 3610
Size: 181 163 5318
Fragments arranged by size:
5318 181 163
BtsI GCAGTG_nn'
Cuts at: 2583 2623 2903 2583
Size: 40 280 5342
Fragments arranged by size:
5342 280 40
ClaI AT'CG_AT
Cuts at: 680 680
Size: 5662
DraI TTT'AAA
Cuts at: 2659 2659
Size: 5662
DrdI GACnn_nn'nnGTC
Cuts at: 1426 3366 4226 1426
Size: 1940 860 2862
Fragments arranged by size:
2862 1940 860
EaeI y'GGCC_r
Cuts at: 509 730 1106 3245 3419 3810 3837 5184 509
Size: 221 376 2139 174 391 27 1347 987
Fragments arranged by size:
2139 1347 987 391 376 221 174 27
EagI C'GGCC_G
Cuts at: 730 3245 730
Size: 2515 3147
EarI CTCTTCn'nnn_
Cuts at: 1366 2209 3683 3893 1366
Size: 843 1474 210 3135
Fragments arranged by size:
3135 1474 843 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3868 4180 4326 5472 3868
Size: 312 146 1146 4058
Fragments arranged by size:
4058 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2385 2672 2385
Size: 287 5375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 496 874 3485 3917 4666 4934 429
Size: 67 378 2611 432 749 268 1157
Fragments arranged by size:
2611 1157 749 432 378 268 67
EcoNI CCTnn'n_nnAGG
Cuts at: 489 715 2440 489
Size: 226 1725 3711
Fragments arranged by size:
3711 1725 226
EcoO109I rG'GnC_Cy
Cuts at: 307 792 1088 1461 1578 2196 2505 2541 2799
Size: 485 296 373 117 618 309 36 258
Cuts at: 2799 307
Size: 3170
Fragments arranged by size:
3170 618 485 373 309 296 258 117
36
EcoRI G'AATT_C
Cuts at: 2664 2664
Size: 5662
EcoRV GAT'ATC
Cuts at: 2265 2265
Size: 5662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3679 3679
Size: 5662
FspI TGC'GCA
Cuts at: 232 3441 232
Size: 3209 2453
HaeII r_GCGC'y
Cuts at: 153 228 1037 1662 2522 3094 3342 4366 5345
Size: 75 809 625 860 572 248 1024 979
Cuts at: 5345 153
Size: 470
Fragments arranged by size:
1024 979 860 809 625 572 470 248
75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 472 504 679 711 718 750 940 972 1213
Size: 32 175 32 7 32 190 32 241
Cuts at: 1213 1245 1900 1932 2249 2281 3502 3532 3534
Size: 32 655 32 317 32 1221 30 2
Cuts at: 3534 3564 472
Size: 30 2570
Fragments arranged by size:
2570 1221 655 317 241 190 175 32
32 32 32 32 32 32 30 30
7 2
HincII GTy'rAC
Cuts at: 2699 2699
Size: 5662
KasI G'GCGC_C
Cuts at: 149 1033 3090 3338 149
Size: 884 2057 248 2473
Fragments arranged by size:
2473 2057 884 248
KpnI G_GTAC'C
Cuts at: 2680 2680
Size: 5662
MluI A'CGCG_T
Cuts at: 5095 5095
Size: 5662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 302 728 1127 1214 1220 2190 2360 4333 4517
Size: 426 399 87 6 970 170 1973 184
Cuts at: 4517 302
Size: 1447
Fragments arranged by size:
1973 1447 970 426 399 184 170 87
6
MscI TGG'CCA
Cuts at: 1108 3421 1108
Size: 2313 3349
MslI CAynn'nnrTG
Cuts at: 286 1604 1753 3649 3776 286
Size: 1318 149 1896 127 2172
Fragments arranged by size:
2172 1896 1318 149 127
MspA1I CmG'CkG
Cuts at: 375 870 894 1031 1295 2498 2771 3085 3445
Size: 495 24 137 264 1203 273 314 360
Cuts at: 3445 4460 4705 375
Size: 1015 245 1332
Fragments arranged by size:
1332 1203 1015 495 360 314 273 264
245 137 24
NaeI GCC'GGC
Cuts at: 1471 2032 3841 1471
Size: 561 1809 3292
Fragments arranged by size:
3292 1809 561
NarI GG'CG_CC
Cuts at: 150 1034 3091 3339 150
Size: 884 2057 248 2473
Fragments arranged by size:
2473 2057 884 248
NcoI C'CATG_G
Cuts at: 1562 1562
Size: 5662
NgoMIV G'CCGG_C
Cuts at: 1469 2030 3839 1469
Size: 561 1809 3292
Fragments arranged by size:
3292 1809 561
NruI TCG'CGA
Cuts at: 4023 4023
Size: 5662
NspI r_CATG'y
Cuts at: 258 1758 2193 2421 2713 3213 4122 258
Size: 1500 435 228 292 500 909 1798
Fragments arranged by size:
1798 1500 909 500 435 292 228
PasI CC'CwG_GG
Cuts at: 1678 1678
Size: 5662
PciI A'CATG_T
Cuts at: 1754 4118 1754
Size: 2364 3298
PflMI CCAn_nnn'nTGG
Cuts at: 2358 2358
Size: 5662
PmeI GTTT'AAAC
Cuts at: 2659 2659
Size: 5662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4801 4833 4801
Size: 32 5630
PpuMI rG'GwC_Cy
Cuts at: 2196 2541 2196
Size: 345 5317
PspOMI G'GGCC_C
Cuts at: 584 791 584
Size: 207 5455
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2203 2235 2203
Size: 32 5630
PstI C_TGCA'G
Cuts at: 1216 1454 2630 2707 3392 1216
Size: 238 1176 77 685 3486
Fragments arranged by size:
3486 1176 685 238 77
PvuI CG_AT'CG
Cuts at: 104 1964 104
Size: 1860 3802
PvuII CAG'CTG
Cuts at: 375 1295 3085 3445 375
Size: 920 1790 360 2592
Fragments arranged by size:
2592 1790 920 360
RsrII CG'GwC_CG
Cuts at: 3855 3855
Size: 5662
SacI G_AGCT'C
Cuts at: 2387 2674 2387
Size: 287 5375
SalI G'TCGA_C
Cuts at: 2697 2697
Size: 5662
SapI GCTCTTCn'nnn_
Cuts at: 2209 3683 3893 2209
Size: 1474 210 3978
Fragments arranged by size:
3978 1474 210
SbfI CC_TGCA'GG
Cuts at: 2707 2707
Size: 5662
ScaI AGT'ACT
Cuts at: 2218 5179 2218
Size: 2961 2701
SfcI C'TryA_G
Cuts at: 33 1212 1450 2626 2703 3388 4383 4574 33
Size: 1179 238 1176 77 685 995 191 1121
Fragments arranged by size:
1179 1176 1121 995 685 238 191 77
SfiI GGCCn_nnn'nGGCC
Cuts at: 1086 1086
Size: 5662
SfoI GGC'GCC
Cuts at: 151 1035 3092 3340 151
Size: 884 2057 248 2473
Fragments arranged by size:
2473 2057 884 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5662
SmaI CCC'GGG
Cuts at: 589 1054 1980 2682 589
Size: 465 926 702 3569
Fragments arranged by size:
3569 926 702 465
SmlI C'TyrA_G
Cuts at: 427 961 2300 2386 2509 2815 3730 4224 4486
Size: 534 1339 86 123 306 915 494 262
Cuts at: 4486 427
Size: 1603
Fragments arranged by size:
1603 1339 915 534 494 306 262 123
86
SnaBI TAC'GTA
Cuts at: 2652 2652
Size: 5662
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5662
SphI G_CATG'C
Cuts at: 2193 2713 3213 2193
Size: 520 500 4642
Fragments arranged by size:
4642 520 500
StyI C'CwwG_G
Cuts at: 990 1562 1611 2794 3148 990
Size: 572 49 1183 354 3504
Fragments arranged by size:
3504 1183 572 354 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 927 3243 3376 3835 4046 927
Size: 2316 133 459 211 2543
Fragments arranged by size:
2543 2316 459 211 133
TatI w'GTAC_w
Cuts at: 674 1757 2216 5177 674
Size: 1083 459 2961 1159
Fragments arranged by size:
2961 1159 1083 459
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 371 403 5179 5211 371
Size: 32 4776 32 822
Fragments arranged by size:
4776 822 32 32
Tth111I GACn'n_nGTC
Cuts at: 1534 3457 1534
Size: 1923 3739
XbaI T'CTAG_A
Cuts at: 2691 2691
Size: 5662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 471 1719 3155 471
Size: 1248 1436 2978
Fragments arranged by size:
2978 1436 1248
XhoI C'TCGA_G
Cuts at: 2386 2386
Size: 5662
XmaI C'CCGG_G
Cuts at: 587 1052 1978 2680 587
Size: 465 926 702 3569
Fragments arranged by size:
3569 926 702 465
XmnI GAAnn'nnTTC
Cuts at: 1360 1510 2314 1360
Size: 150 804 4708
Fragments arranged by size:
4708 804 150
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI
PciI PflMI PmeI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AloI AscI AvrII BaeI BbsI
BfrBI BmtI BsiWI BsmI BsmBI BssHII BstZ17I CspCI
DraIII FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NsiI PacI PfoI PmlI PshAI PsiI PspXI
SacII SanDI SexAI SgrAI SrfI SspI StuI SwaI
ZraI