Restriction map for
pF1KSDA0764
(Circular) (Six-base) MAPSORT of: pF1KSDA0764.seq Check: 2861 from: 1 to: 4381
pF1KSDA0764 4381 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AatII G_ACGT'C
Cuts at: 351 351
Size: 4381
AccI GT'mk_AC
Cuts at: 1417 1417
Size: 4381
Acc65I G'GTAC_C
Cuts at: 1395 1395
Size: 4381
AclI AA'CG_TT
Cuts at: 4221 4221
Size: 4381
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 908 2204 2636 3385 3653 434
Size: 474 1296 432 749 268 1162
Fragments arranged by size:
1296 1162 749 474 432 268
AfeI AGC'GCT
Cuts at: 1342 1342
Size: 4381
AflII C'TTAA_G
Cuts at: 697 697
Size: 4381
AflIII A'CryG_T
Cuts at: 1731 2837 3814 1731
Size: 1106 977 2298
Fragments arranged by size:
2298 1106 977
AgeI A'CCGG_T
Cuts at: 2769 2769
Size: 4381
AlwNI CAG_nnn'CTG
Cuts at: 1029 1684 3253 1029
Size: 655 1569 2157
Fragments arranged by size:
2157 1569 655
ApaLI G'TGCA_C
Cuts at: 3151 3151
Size: 4381
ApoI r'AATT_y
Cuts at: 1383 3734 3990 1383
Size: 2351 256 1774
Fragments arranged by size:
2351 1774 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4381
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4381
AvaI C'yCGr_G
Cuts at: 1399 1399
Size: 4381
BamHI G'GATC_C
Cuts at: 1404 1404
Size: 4381
BanI G'GyrC_C
Cuts at: 1395 1809 2057 2092 1395
Size: 414 248 35 3684
Fragments arranged by size:
3684 414 248 35
BanII G_rGCy'C
Cuts at: 1053 1267 1393 2423 1053
Size: 214 126 1030 3011
Fragments arranged by size:
3011 1030 214 126
BbeI G_GCGC'C
Cuts at: 1813 2061 1813
Size: 248 4133
BbsI GAAGACnn'nnnn_
Cuts at: 343 1209 343
Size: 866 3515
BbvCI CC'TCA_GC
Cuts at: 252 252
Size: 4381
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 568 602 568
Size: 34 4347
BciVI GTATCCnnnnn_n'
Cuts at: 2277 3040 4381 2277
Size: 763 1341 2277
Fragments arranged by size:
2277 1341 763
BclI T'GATC_A
Cuts at: 1899 1899
Size: 4381
BglII A'GATC_T
Cuts at: 1894 3933 1894
Size: 2039 2342
BlpI GC'TnA_GC
Cuts at: 1491 1491
Size: 4381
Bme1580I G_kGCm'C
Cuts at: 442 2004 2097 3155 442
Size: 1562 93 1058 1668
Fragments arranged by size:
1668 1562 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 153 1591 1672 2007 4153 153
Size: 1438 81 335 2146 381
Fragments arranged by size:
2146 1438 381 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 443 2746 443
Size: 2303 2078
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 802 1018 1037 1099 682
Size: 120 216 19 62 3964
Fragments arranged by size:
3964 216 120 62 19
Bpu10I CC'TnA_GC
Cuts at: 252 374 252
Size: 122 4259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1148 1555 2434 2928 3226 1148
Size: 407 879 494 298 2303
Fragments arranged by size:
2303 879 494 407 298
BsaI GGTCTCn'nnnn_
Cuts at: 658 1013 4085 658
Size: 355 3072 954
Fragments arranged by size:
3072 954 355
BsaAI yAC'GTr
Cuts at: 1371 1732 1371
Size: 361 4020
BsaBI GATnn'nnATC
Cuts at: 6 1893 3932 6
Size: 1887 2039 455
Fragments arranged by size:
2039 1887 455
BsaHI Gr'CG_yC
Cuts at: 348 1810 2058 2762 4258 348
Size: 1462 248 704 1496 471
Fragments arranged by size:
1496 1462 704 471 248
BsaWI w'CCGG_w
Cuts at: 1568 2089 2769 3043 3190 3601 1568
Size: 521 680 274 147 411 2348
Fragments arranged by size:
2348 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 440 470 1216 1246 440
Size: 30 746 30 3575
Fragments arranged by size:
3575 746 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 443 586 1006 1118 1237 443
Size: 143 420 112 119 3587
Fragments arranged by size:
3587 420 143 119 112
BseYI C'CCAG_C
Cuts at: 744 1203 1249 1804 2562 3141 3637 744
Size: 459 46 555 758 579 496 1488
Fragments arranged by size:
1488 758 579 555 496 459 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 1130 226
Size: 904 3477
BsiEI CG_ry'CG
Cuts at: 104 1921 1967 3177 104
Size: 1817 46 1210 1308
Fragments arranged by size:
1817 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 812 1393 2171 2361 3155 812
Size: 581 778 190 794 2038
Fragments arranged by size:
2038 794 778 581 190
Bsp1286I G_dGCh'C
Cuts at: 442 812 1053 1267 1393 2004 2097 2171 2361
Size: 370 241 214 126 611 93 74 190
Cuts at: 2361 2423 3155 442
Size: 62 732 1668
Fragments arranged by size:
1668 732 611 370 241 214 190 126
93 74 62
BspEI T'CCGG_A
Cuts at: 3601 3601
Size: 4381
BspHI T'CATG_A
Cuts at: 3557 3557
Size: 4381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 768 1429 1643 1945 2326 768
Size: 661 214 302 381 2823
Fragments arranged by size:
2823 661 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2671 2725 4211 4376 50
Size: 2621 54 1486 165 55
Fragments arranged by size:
2621 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1188 2291 1188
Size: 1103 3278
BsrFI r'CCGG_y
Cuts at: 2377 2558 2769 2377
Size: 181 211 3989
Fragments arranged by size:
3989 211 181
BssSI C'ACGA_G
Cuts at: 435 2650 3010 435
Size: 2215 360 1806
Fragments arranged by size:
2215 1806 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1200 1756 1200
Size: 556 3825
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4381
BstEII G'GTnAC_C
Cuts at: 446 446
Size: 4381
BstXI CCAn_nnnn'nTGG
Cuts at: 282 621 282
Size: 339 4042
BstYI r'GATC_y
Cuts at: 1 509 1404 1871 1894 2229 2475 3489 3575
Size: 508 895 467 23 335 246 1014 86
Cuts at: 3575 3587 3933 1
Size: 12 346 449
Fragments arranged by size:
1014 895 508 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 202 202
Size: 4381
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2329 2510 2673 2329
Size: 181 163 4037
Fragments arranged by size:
4037 181 163
BtsI GCAGTG_nn'
Cuts at: 614 810 1027 1215 1622 614
Size: 196 217 188 407 3373
Fragments arranged by size:
3373 407 217 196 188
DraI TTT'AAA
Cuts at: 1378 1378
Size: 4381
DrdI GACnn_nn'nnGTC
Cuts at: 2085 2945 2085
Size: 860 3521
EaeI y'GGCC_r
Cuts at: 611 1964 2138 2529 2556 3903 611
Size: 1353 174 391 27 1347 1089
Fragments arranged by size:
1353 1347 1089 391 174 27
EagI C'GGCC_G
Cuts at: 1964 1964
Size: 4381
EarI CTCTTCn'nnn_
Cuts at: 410 434 1345 2402 2612 410
Size: 24 911 1057 210 2179
Fragments arranged by size:
2179 1057 911 210 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2587 2899 3045 4191 2587
Size: 312 146 1146 2777
Fragments arranged by size:
2777 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1391 1391
Size: 4381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 682 802 908 1018 1037 1099 2204 2636
Size: 248 120 106 110 19 62 1105 432
Cuts at: 2636 3385 3653 434
Size: 749 268 1162
Fragments arranged by size:
1162 1105 749 432 268 248 120 110
106 62 19
EcoNI CCTnn'n_nnAGG
Cuts at: 601 1232 601
Size: 631 3750
EcoO109I rG'GnC_Cy
Cuts at: 1319 1518 1319
Size: 199 4182
EcoRI G'AATT_C
Cuts at: 1383 1383
Size: 4381
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4381
FspI TGC'GCA
Cuts at: 2160 2160
Size: 4381
HaeII r_GCGC'y
Cuts at: 1344 1813 2061 3085 4064 1344
Size: 469 248 1024 979 1661
Fragments arranged by size:
1661 1024 979 469 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 277 309 894 926 1216 1248 2221 2251 2253
Size: 32 585 32 290 32 973 30 2
Cuts at: 2253 2283 277
Size: 30 2375
Fragments arranged by size:
2375 973 585 290 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1418 1418
Size: 4381
HindIII A'AGCT_T
Cuts at: 1088 1088
Size: 4381
KasI G'GCGC_C
Cuts at: 1809 2057 1809
Size: 248 4133
KpnI G_GTAC'C
Cuts at: 1399 1399
Size: 4381
MluI A'CGCG_T
Cuts at: 3814 3814
Size: 4381
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3052 3236 3052
Size: 184 4197
MscI TGG'CCA
Cuts at: 613 2140 613
Size: 1527 2854
MslI CAynn'nnrTG
Cuts at: 2368 2495 2368
Size: 127 4254
MspA1I CmG'CkG
Cuts at: 575 1490 1804 2164 3179 3424 575
Size: 915 314 360 1015 245 1532
Fragments arranged by size:
1532 1015 915 360 314 245
NaeI GCC'GGC
Cuts at: 2560 2560
Size: 4381
NarI GG'CG_CC
Cuts at: 1810 2058 1810
Size: 248 4133
NgoMIV G'CCGG_C
Cuts at: 2558 2558
Size: 4381
NruI TCG'CGA
Cuts at: 2742 2742
Size: 4381
NspI r_CATG'y
Cuts at: 864 1432 1932 2841 864
Size: 568 500 909 2404
Fragments arranged by size:
2404 909 568 500
PasI CC'CwG_GG
Cuts at: 442 442
Size: 4381
PciI A'CATG_T
Cuts at: 2837 2837
Size: 4381
PflMI CCAn_nnn'nTGG
Cuts at: 609 792 609
Size: 183 4198
PfoI T'CCnGG_A
Cuts at: 659 659
Size: 4381
PmeI GTTT'AAAC
Cuts at: 1378 1378
Size: 4381
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3520 3552 3520
Size: 32 4349
PpuMI rG'GwC_Cy
Cuts at: 1319 1319
Size: 4381
PstI C_TGCA'G
Cuts at: 1426 2111 1426
Size: 685 3696
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4381
PvuII CAG'CTG
Cuts at: 575 1804 2164 575
Size: 1229 360 2792
Fragments arranged by size:
2792 1229 360
RsrII CG'GwC_CG
Cuts at: 2574 2574
Size: 4381
SacI G_AGCT'C
Cuts at: 1393 1393
Size: 4381
SalI G'TCGA_C
Cuts at: 1416 1416
Size: 4381
SapI GCTCTTCn'nnn_
Cuts at: 2402 2612 2402
Size: 210 4171
SbfI CC_TGCA'GG
Cuts at: 1426 1426
Size: 4381
ScaI AGT'ACT
Cuts at: 3898 3898
Size: 4381
SfcI C'TryA_G
Cuts at: 33 865 1422 2107 3102 3293 33
Size: 832 557 685 995 191 1121
Fragments arranged by size:
1121 995 832 685 557 191
SfoI GGC'GCC
Cuts at: 1811 2059 1811
Size: 248 4133
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4381
SmaI CCC'GGG
Cuts at: 1401 1401
Size: 4381
SmlI C'TyrA_G
Cuts at: 697 1163 1534 2449 2943 3205 697
Size: 466 371 915 494 262 1873
Fragments arranged by size:
1873 915 494 466 371 262
SnaBI TAC'GTA
Cuts at: 1371 1371
Size: 4381
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4381
SphI G_CATG'C
Cuts at: 1432 1932 1432
Size: 500 3881
StyI C'CwwG_G
Cuts at: 1513 1867 1513
Size: 354 4027
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1962 2095 2554 2765 1962
Size: 133 459 211 3578
Fragments arranged by size:
3578 459 211 133
TatI w'GTAC_w
Cuts at: 3896 3896
Size: 4381
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 194 226 440 472 3898 3930 194
Size: 32 214 32 3426 32 645
Fragments arranged by size:
3426 645 214 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2176 2176
Size: 4381
XbaI T'CTAG_A
Cuts at: 1410 1410
Size: 4381
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1874 1874
Size: 4381
XmaI C'CCGG_G
Cuts at: 1399 1399
Size: 4381
XmnI GAAnn'nnTTC
Cuts at: 428 428
Size: 4381
ZraI GAC'GTC
Cuts at: 349 349
Size: 4381
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AhdI AleI AlfI AloI ApaI AscI AvrII
BaeI BfrBI BglI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NsiI PacI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SspI StuI
SwaI XhoI