Restriction map for
pF1KSDA0769
(Circular) (Six-base) MAPSORT of: pF1KSDA0769.seq Check: 4224 from: 1 to: 5191
pF1KSDA0769 5191 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1384 1384
Size: 5191
AccI GT'mk_AC
Cuts at: 2227 2227
Size: 5191
Acc65I G'GTAC_C
Cuts at: 2205 2205
Size: 5191
AclI AA'CG_TT
Cuts at: 5031 5031
Size: 5191
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 696 790 1142 1988 3014 3446 4195 4463
Size: 524 94 352 846 1026 432 749 268
Cuts at: 4463 172
Size: 900
Fragments arranged by size:
1026 900 846 749 524 432 352 268
94
AflII C'TTAA_G
Cuts at: 1356 1356
Size: 5191
AflIII A'CryG_T
Cuts at: 955 957 1601 2541 3647 4624 955
Size: 2 644 940 1106 977 1522
Fragments arranged by size:
1522 1106 977 940 644 2
AgeI A'CCGG_T
Cuts at: 3579 3579
Size: 5191
AhdI GACnn_n'nnGTC
Cuts at: 1593 1593
Size: 5191
AleI CACnn'nnGTG
Cuts at: 958 2173 958
Size: 1215 3976
AlwNI CAG_nnn'CTG
Cuts at: 904 1575 2494 4063 904
Size: 671 919 1569 2032
Fragments arranged by size:
2032 1569 919 671
ApaI G_GGCC'C
Cuts at: 1227 1227
Size: 5191
ApaLI G'TGCA_C
Cuts at: 3961 3961
Size: 5191
ApoI r'AATT_y
Cuts at: 1789 2193 4544 4800 1789
Size: 404 2351 256 2180
Fragments arranged by size:
2351 2180 404 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5191
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5191
AvaI C'yCGr_G
Cuts at: 229 2209 229
Size: 1980 3211
BamHI G'GATC_C
Cuts at: 2214 2214
Size: 5191
BanI G'GyrC_C
Cuts at: 1351 2205 2619 2867 2902 1351
Size: 854 414 248 35 3640
Fragments arranged by size:
3640 854 414 248 35
BanII G_rGCy'C
Cuts at: 1227 1981 2203 3233 1227
Size: 754 222 1030 3185
Fragments arranged by size:
3185 1030 754 222
BbeI G_GCGC'C
Cuts at: 2623 2871 2623
Size: 248 4943
BbsI GAAGACnn'nnnn_
Cuts at: 1478 1478
Size: 5191
BciVI GTATCCnnnnn_n'
Cuts at: 218 3087 3850 5191 218
Size: 2869 763 1341 218
Fragments arranged by size:
2869 1341 763 218
BclI T'GATC_A
Cuts at: 684 2709 684
Size: 2025 3166
BglI GCCn_nnn'nGGC
Cuts at: 1349 1349
Size: 5191
BglII A'GATC_T
Cuts at: 1883 2704 4743 1883
Size: 821 2039 2331
Fragments arranged by size:
2331 2039 821
BlpI GC'TnA_GC
Cuts at: 2301 2301
Size: 5191
Bme1580I G_kGCm'C
Cuts at: 1227 2814 2907 3965 1227
Size: 1587 93 1058 2453
Fragments arranged by size:
2453 1587 1058 93
BmgBI CAC'GTC
Cuts at: 1604 1604
Size: 5191
BmrI ACTGGGnnnn_n'
Cuts at: 246 914 1485 2401 2482 2817 4963 246
Size: 668 571 916 81 335 2146 474
Fragments arranged by size:
2146 916 668 571 474 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3556 3556
Size: 5191
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 762 1033 188
Size: 574 271 4346
Fragments arranged by size:
4346 574 271
Bpu10I CC'TnA_GC
Cuts at: 1558 1558
Size: 5191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 2365 3244 3738 4036 676
Size: 1689 879 494 298 1831
Fragments arranged by size:
1831 1689 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 2003 2065 4895 2003
Size: 62 2830 2299
Fragments arranged by size:
2830 2299 62
BsaAI yAC'GTr
Cuts at: 958 967 2181 2542 958
Size: 9 1214 361 3607
Fragments arranged by size:
3607 1214 361 9
BsaBI GATnn'nnATC
Cuts at: 6 1882 2703 4742 6
Size: 1876 821 2039 455
Fragments arranged by size:
2039 1876 821 455
BsaHI Gr'CG_yC
Cuts at: 2620 2868 3572 5068 2620
Size: 248 704 1496 2743
Fragments arranged by size:
2743 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2378 2899 3579 3853 4000 4411 2378
Size: 521 680 274 147 411 3158
Fragments arranged by size:
3158 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1016 1046 1016
Size: 30 5161
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 931 1989 931
Size: 1058 4133
BseYI C'CCAG_C
Cuts at: 845 1945 1960 2614 3372 3951 4447 845
Size: 1100 15 654 758 579 496 1589
Fragments arranged by size:
1589 1100 758 654 579 496 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 766
Size: 5191
BsiEI CG_ry'CG
Cuts at: 104 2731 2777 3987 104
Size: 2627 46 1210 1308
Fragments arranged by size:
2627 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 923 1981 2203 2981 3171 3965 923
Size: 1058 222 778 190 794 2149
Fragments arranged by size:
2149 1058 794 778 222 190
Bsp1286I G_dGCh'C
Cuts at: 923 1227 1981 2203 2814 2907 2981 3171 3233
Size: 304 754 222 611 93 74 190 62
Cuts at: 3233 3965 923
Size: 732 2149
Fragments arranged by size:
2149 754 732 611 304 222 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4411 4411
Size: 5191
BspHI T'CATG_A
Cuts at: 4367 4367
Size: 5191
BspMI ACCTGCnnnn'nnnn_
Cuts at: 234 1145 1384 2239 2453 2755 3136 234
Size: 911 239 855 214 302 381 2289
Fragments arranged by size:
2289 911 855 381 302 239 214
BsrBI CCG'CTC
Cuts at: 50 3481 3535 5021 5186 50
Size: 3431 54 1486 165 55
Fragments arranged by size:
3431 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3101 3101
Size: 5191
BsrFI r'CCGG_y
Cuts at: 3187 3368 3579 3187
Size: 181 211 4799
Fragments arranged by size:
4799 211 181
BssSI C'ACGA_G
Cuts at: 3460 3820 3460
Size: 360 4831
BstAPI GCAn_nnn'nTGC
Cuts at: 613 2566 613
Size: 1953 3238
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5191
BstXI CCAn_nnnn'nTGG
Cuts at: 786 960 786
Size: 174 5017
BstYI r'GATC_y
Cuts at: 1 365 1883 2214 2681 2704 3039 3285 4299
Size: 364 1518 331 467 23 335 246 1014
Cuts at: 4299 4385 4397 4743 1
Size: 86 12 346 449
Fragments arranged by size:
1518 1014 467 449 364 346 335 331
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1724 1724
Size: 5191
BtgI C'CryG_G
Cuts at: 1616 1864 1616
Size: 248 4943
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1233 3139 3320 3483 1233
Size: 1906 181 163 2941
Fragments arranged by size:
2941 1906 181 163
BtsI GCAGTG_nn'
Cuts at: 1244 1921 2432 1244
Size: 677 511 4003
Fragments arranged by size:
4003 677 511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1910 1945 1910
Size: 35 5156
DraI TTT'AAA
Cuts at: 2188 2188
Size: 5191
DrdI GACnn_nn'nnGTC
Cuts at: 2895 3755 2895
Size: 860 4331
EaeI y'GGCC_r
Cuts at: 1500 1577 2774 2948 3339 3366 4713 1500
Size: 77 1197 174 391 27 1347 1978
Fragments arranged by size:
1978 1347 1197 391 174 77 27
EagI C'GGCC_G
Cuts at: 2774 2774
Size: 5191
EarI CTCTTCn'nnn_
Cuts at: 148 1292 3212 3422 148
Size: 1144 1920 210 1917
Fragments arranged by size:
1920 1917 1144 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3397 3709 3855 5001 3397
Size: 312 146 1146 3587
Fragments arranged by size:
3587 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1979 2201 1979
Size: 222 4969
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 188 696 762 790 1033 1142 1988 3014
Size: 16 508 66 28 243 109 846 1026
Cuts at: 3014 3446 4195 4463 172
Size: 432 749 268 900
Fragments arranged by size:
1026 900 846 749 508 432 268 243
109 66 28 16
EcoO109I rG'GnC_Cy
Cuts at: 2328 2328
Size: 5191
EcoRI G'AATT_C
Cuts at: 1789 2193 1789
Size: 404 4787
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 438 3208 438
Size: 2770 2421
FspI TGC'GCA
Cuts at: 2970 2970
Size: 5191
HaeII r_GCGC'y
Cuts at: 2623 2871 3895 4874 2623
Size: 248 1024 979 2940
Fragments arranged by size:
2940 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 192 224 463 495 1579 1611 1699 1721 1731
Size: 32 239 32 1084 32 88 22 10
Cuts at: 1731 1753 3031 3061 3063 3093 192
Size: 22 1278 30 2 30 2290
Fragments arranged by size:
2290 1278 1084 239 88 32 32 32
30 30 22 22 10 2
HincII GTy'rAC
Cuts at: 359 651 1421 2228 359
Size: 292 770 807 3322
Fragments arranged by size:
3322 807 770 292
HindIII A'AGCT_T
Cuts at: 318 318
Size: 5191
HpaI GTT'AAC
Cuts at: 651 651
Size: 5191
KasI G'GCGC_C
Cuts at: 2619 2867 2619
Size: 248 4943
KpnI G_GTAC'C
Cuts at: 2209 2209
Size: 5191
MluI A'CGCG_T
Cuts at: 4624 4624
Size: 5191
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1112 3862 4046 1112
Size: 2750 184 2257
Fragments arranged by size:
2750 2257 184
MscI TGG'CCA
Cuts at: 1502 2950 1502
Size: 1448 3743
MslI CAynn'nnrTG
Cuts at: 958 2173 3178 3305 958
Size: 1215 1005 127 2844
Fragments arranged by size:
2844 1215 1005 127
MspA1I CmG'CkG
Cuts at: 810 2300 2614 2974 3989 4234 810
Size: 1490 314 360 1015 245 1767
Fragments arranged by size:
1767 1490 1015 360 314 245
NaeI GCC'GGC
Cuts at: 3370 3370
Size: 5191
NarI GG'CG_CC
Cuts at: 2620 2868 2620
Size: 248 4943
NcoI C'CATG_G
Cuts at: 1864 1864
Size: 5191
NdeI CA'TA_TG
Cuts at: 2079 2079
Size: 5191
NgoMIV G'CCGG_C
Cuts at: 3368 3368
Size: 5191
NruI TCG'CGA
Cuts at: 3552 3552
Size: 5191
NspI r_CATG'y
Cuts at: 207 737 1566 2242 2742 3651 207
Size: 530 829 676 500 909 1747
Fragments arranged by size:
1747 909 829 676 530 500
PciI A'CATG_T
Cuts at: 3647 3647
Size: 5191
PflMI CCAn_nnn'nTGG
Cuts at: 1197 1197
Size: 5191
PfoI T'CCnGG_A
Cuts at: 791 791
Size: 5191
PmeI GTTT'AAAC
Cuts at: 2188 2188
Size: 5191
PmlI CAC'GTG
Cuts at: 958 967 958
Size: 9 5182
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4330 4362 4330
Size: 32 5159
PspOMI G'GGCC_C
Cuts at: 1223 1223
Size: 5191
PstI C_TGCA'G
Cuts at: 1239 1571 2236 2921 1239
Size: 332 665 685 3509
Fragments arranged by size:
3509 685 665 332
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5191
PvuII CAG'CTG
Cuts at: 810 2614 2974 810
Size: 1804 360 3027
Fragments arranged by size:
3027 1804 360
RsrII CG'GwC_CG
Cuts at: 3384 3384
Size: 5191
SacI G_AGCT'C
Cuts at: 1981 2203 1981
Size: 222 4969
SalI G'TCGA_C
Cuts at: 2226 2226
Size: 5191
SapI GCTCTTCn'nnn_
Cuts at: 3212 3422 3212
Size: 210 4981
SbfI CC_TGCA'GG
Cuts at: 2236 2236
Size: 5191
ScaI AGT'ACT
Cuts at: 4708 4708
Size: 5191
SfcI C'TryA_G
Cuts at: 33 1235 1531 1567 2232 2917 3912 4103 33
Size: 1202 296 36 665 685 995 191 1121
Fragments arranged by size:
1202 1121 995 685 665 296 191 36
SfoI GGC'GCC
Cuts at: 2621 2869 2621
Size: 248 4943
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5191
SmaI CCC'GGG
Cuts at: 2211 2211
Size: 5191
SmlI C'TyrA_G
Cuts at: 229 691 1356 2344 3259 3753 4015 229
Size: 462 665 988 915 494 262 1405
Fragments arranged by size:
1405 988 915 665 494 462 262
SnaBI TAC'GTA
Cuts at: 2181 2181
Size: 5191
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5191
SphI G_CATG'C
Cuts at: 1566 2242 2742 1566
Size: 676 500 4015
Fragments arranged by size:
4015 676 500
SspI AAT'ATT
Cuts at: 1089 1089
Size: 5191
StyI C'CwwG_G
Cuts at: 1864 2323 2677 1864
Size: 459 354 4378
Fragments arranged by size:
4378 459 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1583 2772 2905 3364 3575 1583
Size: 1189 133 459 211 3199
Fragments arranged by size:
3199 1189 459 211 133
TatI w'GTAC_w
Cuts at: 1985 4706 1985
Size: 2721 2470
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4708 4740 4708
Size: 32 5159
Tth111I GACn'n_nGTC
Cuts at: 2986 2986
Size: 5191
XbaI T'CTAG_A
Cuts at: 2220 2220
Size: 5191
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 1768 2684 132
Size: 1636 916 2639
Fragments arranged by size:
2639 1636 916
XhoI C'TCGA_G
Cuts at: 229 229
Size: 5191
XmaI C'CCGG_G
Cuts at: 2209 2209
Size: 5191
XmnI GAAnn'nnTTC
Cuts at: 754 1088 754
Size: 334 4857
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NruI NspI PciI PflMI PfoI
PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AlfI AloI AscI AvrII BaeI BbvCI
BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI
MfeI NheI NotI NsiI PacI PasI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI StuI SwaI ZraI