Restriction map for
pF1KSDA0770
(Circular) (Six-base) MAPSORT of: pF1KSDA0770.seq Check: 8807 from: 1 to: 5764
pF1KSDA0770 5764 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1641 2604 1641
Size: 963 4801
AccI GT'mk_AC
Cuts at: 2800 2800
Size: 5764
Acc65I G'GTAC_C
Cuts at: 2778 2778
Size: 5764
AclI AA'CG_TT
Cuts at: 5604 5604
Size: 5764
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1822 1835 3587 4019 4768 5036 1822
Size: 13 1752 432 749 268 2550
Fragments arranged by size:
2550 1752 749 432 268 13
AflIII A'CryG_T
Cuts at: 3114 4220 5197 3114
Size: 1106 977 3681
Fragments arranged by size:
3681 1106 977
AgeI A'CCGG_T
Cuts at: 478 4152 478
Size: 3674 2090
AleI CACnn'nnGTG
Cuts at: 1225 1225
Size: 5764
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 949 1214 949
Size: 265 5499
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1898 1930 2156 2188 1898
Size: 32 226 32 5474
Fragments arranged by size:
5474 226 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1719 1728 3067 4636 1719
Size: 9 1339 1569 2847
Fragments arranged by size:
2847 1569 1339 9
ApaLI G'TGCA_C
Cuts at: 4534 4534
Size: 5764
ApoI r'AATT_y
Cuts at: 550 615 1124 1875 2575 2766 5117 5373 550
Size: 65 509 751 700 191 2351 256 941
Fragments arranged by size:
2351 941 751 700 509 256 191 65
AseI AT'TA_AT
Cuts at: 20 20
Size: 5764
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5764
AvaI C'yCGr_G
Cuts at: 2416 2782 2416
Size: 366 5398
BamHI G'GATC_C
Cuts at: 2787 2787
Size: 5764
BanI G'GyrC_C
Cuts at: 2778 3192 3440 3475 2778
Size: 414 248 35 5067
Fragments arranged by size:
5067 414 248 35
BanII G_rGCy'C
Cuts at: 689 716 1634 2172 2776 3806 689
Size: 27 918 538 604 1030 2647
Fragments arranged by size:
2647 1030 918 604 538 27
BbeI G_GCGC'C
Cuts at: 3196 3444 3196
Size: 248 5516
BbvCI CC'TCA_GC
Cuts at: 2249 2249
Size: 5764
BciVI GTATCCnnnnn_n'
Cuts at: 1292 2229 3660 4423 5764 1292
Size: 937 1431 763 1341 1292
Fragments arranged by size:
1431 1341 1292 937 763
BclI T'GATC_A
Cuts at: 1395 3282 1395
Size: 1887 3877
BglI GCCn_nnn'nGGC
Cuts at: 1519 1519
Size: 5764
BglII A'GATC_T
Cuts at: 1818 3277 5316 1818
Size: 1459 2039 2266
Fragments arranged by size:
2266 2039 1459
BlpI GC'TnA_GC
Cuts at: 2874 2874
Size: 5764
Bme1580I G_kGCm'C
Cuts at: 593 813 1735 3387 3480 4538 593
Size: 220 922 1652 93 1058 1819
Fragments arranged by size:
1819 1652 1058 922 220 93
BmrI ACTGGGnnnn_n'
Cuts at: 339 2026 2974 3055 3390 5536 339
Size: 1687 948 81 335 2146 567
Fragments arranged by size:
2146 1687 948 567 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1953 4129 1953
Size: 2176 3588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 730 1033 1558 2063 2380 2398 448
Size: 282 303 525 505 317 18 3814
Fragments arranged by size:
3814 525 505 317 303 282 18
Bpu10I CC'TnA_GC
Cuts at: 2249 2249
Size: 5764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1471 2539 2938 3817 4311 4609 1471
Size: 1068 399 879 494 298 2626
Fragments arranged by size:
2626 1068 879 494 399 298
BsaI GGTCTCn'nnnn_
Cuts at: 469 5468 469
Size: 4999 765
BsaAI yAC'GTr
Cuts at: 1575 1846 2754 3115 1575
Size: 271 908 361 4224
Fragments arranged by size:
4224 908 361 271
BsaBI GATnn'nnATC
Cuts at: 6 2303 3276 5315 6
Size: 2297 973 2039 455
Fragments arranged by size:
2297 2039 973 455
BsaHI Gr'CG_yC
Cuts at: 3193 3441 4145 5641 3193
Size: 248 704 1496 3316
Fragments arranged by size:
3316 1496 704 248
BsaWI w'CCGG_w
Cuts at: 478 2951 3472 4152 4426 4573 4984 478
Size: 2473 521 680 274 147 411 1258
Fragments arranged by size:
2473 1258 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1031 1061 2156 2186 2629 2659 2693 2723 1031
Size: 30 1095 30 443 30 34 30 4072
Fragments arranged by size:
4072 1095 443 34 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 499 1576 1942 2074 2266 2650 499
Size: 1077 366 132 192 384 3613
Fragments arranged by size:
3613 1077 384 366 192 132
BseYI C'CCAG_C
Cuts at: 2037 2061 2338 2739 3187 3945 4524 5020 2037
Size: 24 277 401 448 758 579 496 2781
Fragments arranged by size:
2781 758 579 496 448 401 277 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 780 1732 780
Size: 952 4812
BsiEI CG_ry'CG
Cuts at: 104 3304 3350 4560 104
Size: 3200 46 1210 1308
Fragments arranged by size:
3200 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 689 848 1304 1574 1658 2172 2555 2776 3554
Size: 159 456 270 84 514 383 221 778
Cuts at: 3554 3744 4538 689
Size: 190 794 1915
Fragments arranged by size:
1915 794 778 514 456 383 270 221
190 159 84
BsmI GAATG_Cn'
Cuts at: 2342 2342
Size: 5764
Bsp1286I G_dGCh'C
Cuts at: 593 689 716 813 848 1304 1574 1634 1658
Size: 96 27 97 35 456 270 60 24
Cuts at: 1658 1735 2172 2555 2776 3387 3480 3554 3744
Size: 77 437 383 221 611 93 74 190
Cuts at: 3744 3806 4538 593
Size: 62 732 1819
Fragments arranged by size:
1819 732 611 456 437 383 270 221
190 97 96 93 77 74 62 60
35 27 24
BspEI T'CCGG_A
Cuts at: 4984 4984
Size: 5764
BspHI T'CATG_A
Cuts at: 555 4940 555
Size: 4385 1379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1182 1256 1641 1751 2400 2604 2812 3026 3328
Size: 74 385 110 649 204 208 214 302
Cuts at: 3328 3709 1182
Size: 381 3237
Fragments arranged by size:
3237 649 385 381 302 214 208 204
110 74
BsrBI CCG'CTC
Cuts at: 50 1419 2404 2598 4054 4108 5594 5759 50
Size: 1369 985 194 1456 54 1486 165 55
Fragments arranged by size:
1486 1456 1369 985 194 165 55 54
BsrDI GCAATG_nn'
Cuts at: 863 1291 3674 863
Size: 428 2383 2953
Fragments arranged by size:
2953 2383 428
BsrFI r'CCGG_y
Cuts at: 478 2072 2123 3760 3941 4152 478
Size: 1594 51 1637 181 211 2090
Fragments arranged by size:
2090 1637 1594 211 181 51
BssSI C'ACGA_G
Cuts at: 1636 1657 1699 4033 4393 1636
Size: 21 42 2334 360 3007
Fragments arranged by size:
3007 2334 360 42 21
BstAPI GCAn_nnn'nTGC
Cuts at: 1719 2348 3139 1719
Size: 629 791 4344
Fragments arranged by size:
4344 791 629
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5764
BstXI CCAn_nnnn'nTGG
Cuts at: 600 2703 600
Size: 2103 3661
BstYI r'GATC_y
Cuts at: 1 329 791 890 1215 1818 2787 3254 3277
Size: 328 462 99 325 603 969 467 23
Cuts at: 3277 3612 3858 4872 4958 4970 5316 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1014 969 603 467 462 449 346 335
328 325 246 99 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 957 2581 957
Size: 1624 4140
BtgI C'CryG_G
Cuts at: 599 770 839 1022 599
Size: 171 69 183 5341
Fragments arranged by size:
5341 183 171 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3712 3893 4056 3712
Size: 181 163 5420
Fragments arranged by size:
5420 181 163
BtsI GCAGTG_nn'
Cuts at: 875 1548 2346 3005 875
Size: 673 798 659 3634
Fragments arranged by size:
3634 798 673 659
DraI TTT'AAA
Cuts at: 2761 2761
Size: 5764
DrdI GACnn_nn'nnGTC
Cuts at: 3468 4328 3468
Size: 860 4904
EaeI y'GGCC_r
Cuts at: 836 983 3347 3521 3912 3939 5286 836
Size: 147 2364 174 391 27 1347 1314
Fragments arranged by size:
2364 1347 1314 391 174 147 27
EagI C'GGCC_G
Cuts at: 3347 3347
Size: 5764
EarI CTCTTCn'nnn_
Cuts at: 1165 2045 2588 3785 3995 1165
Size: 880 543 1197 210 2934
Fragments arranged by size:
2934 1197 880 543 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3970 4282 4428 5574 3970
Size: 312 146 1146 4160
Fragments arranged by size:
4160 1146 312 146
EcoICRI GAG'CTC
Cuts at: 687 2170 2774 687
Size: 1483 604 3677
Fragments arranged by size:
3677 1483 604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 730 1033 1558 1822 1835 2063 2380 2398
Size: 282 303 525 264 13 228 317 18
Cuts at: 2398 3587 4019 4768 5036 448
Size: 1189 432 749 268 1176
Fragments arranged by size:
1189 1176 749 525 432 317 303 282
268 264 228 18 13
EcoNI CCTnn'n_nnAGG
Cuts at: 1691 2506 1691
Size: 815 4949
EcoO109I rG'GnC_Cy
Cuts at: 941 2412 2451 2901 941
Size: 1471 39 450 3804
Fragments arranged by size:
3804 1471 450 39
EcoRI G'AATT_C
Cuts at: 2575 2766 2575
Size: 191 5573
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 250 1347 3781 250
Size: 1097 2434 2233
Fragments arranged by size:
2434 2233 1097
FspI TGC'GCA
Cuts at: 3543 3543
Size: 5764
HaeII r_GCGC'y
Cuts at: 3196 3444 4468 5447 3196
Size: 248 1024 979 3513
Fragments arranged by size:
3513 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1387 1419 1923 1955 2693 2725 3604 3634 3636
Size: 32 504 32 738 32 879 30 2
Cuts at: 3636 3666 1387
Size: 30 3485
Fragments arranged by size:
3485 879 738 504 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2801 2801
Size: 5764
KasI G'GCGC_C
Cuts at: 3192 3440 3192
Size: 248 5516
KpnI G_GTAC'C
Cuts at: 2782 2782
Size: 5764
MfeI C'AATT_G
Cuts at: 1369 1369
Size: 5764
MluI A'CGCG_T
Cuts at: 5197 5197
Size: 5764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 718 1062 4435 4619 718
Size: 344 3373 184 1863
Fragments arranged by size:
3373 1863 344 184
MscI TGG'CCA
Cuts at: 838 985 3523 838
Size: 147 2538 3079
Fragments arranged by size:
3079 2538 147
MslI CAynn'nnrTG
Cuts at: 804 832 868 1225 1505 3751 3878 804
Size: 28 36 357 280 2246 127 2690
Fragments arranged by size:
2690 2246 357 280 127 36 28
MspA1I CmG'CkG
Cuts at: 708 2041 2743 2873 3187 3547 4562 4807 708
Size: 1333 702 130 314 360 1015 245 1665
Fragments arranged by size:
1665 1333 1015 702 360 314 245 130
NaeI GCC'GGC
Cuts at: 2125 3943 2125
Size: 1818 3946
NarI GG'CG_CC
Cuts at: 3193 3441 3193
Size: 248 5516
NcoI C'CATG_G
Cuts at: 599 839 1022 599
Size: 240 183 5341
Fragments arranged by size:
5341 240 183
NgoMIV G'CCGG_C
Cuts at: 2123 3941 2123
Size: 1818 3946
NruI TCG'CGA
Cuts at: 4125 4125
Size: 5764
NspI r_CATG'y
Cuts at: 2815 3315 4224 2815
Size: 500 909 4355
Fragments arranged by size:
4355 909 500
PciI A'CATG_T
Cuts at: 4220 4220
Size: 5764
PflMI CCAn_nnn'nTGG
Cuts at: 605 1230 1795 605
Size: 625 565 4574
Fragments arranged by size:
4574 625 565
PfoI T'CCnGG_A
Cuts at: 1049 1823 2501 2588 1049
Size: 774 678 87 4225
Fragments arranged by size:
4225 774 678 87
PmeI GTTT'AAAC
Cuts at: 2761 2761
Size: 5764
PmlI CAC'GTG
Cuts at: 1575 1846 1575
Size: 271 5493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2156 2188 4903 4935 2156
Size: 32 2715 32 2985
Fragments arranged by size:
2985 2715 32 32
PpuMI rG'GwC_Cy
Cuts at: 2412 2412
Size: 5764
PspXI vC'TCGA_Gb
Cuts at: 2416 2416
Size: 5764
PstI C_TGCA'G
Cuts at: 527 1079 1652 1724 2022 2411 2809 3494 527
Size: 552 573 72 298 389 398 685 2797
Fragments arranged by size:
2797 685 573 552 398 389 298 72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5764
PvuII CAG'CTG
Cuts at: 708 2041 2743 3187 3547 708
Size: 1333 702 444 360 2925
Fragments arranged by size:
2925 1333 702 444 360
RsrII CG'GwC_CG
Cuts at: 3957 3957
Size: 5764
SacI G_AGCT'C
Cuts at: 689 2172 2776 689
Size: 1483 604 3677
Fragments arranged by size:
3677 1483 604
SalI G'TCGA_C
Cuts at: 2799 2799
Size: 5764
SapI GCTCTTCn'nnn_
Cuts at: 2588 3785 3995 2588
Size: 1197 210 4357
Fragments arranged by size:
4357 1197 210
SbfI CC_TGCA'GG
Cuts at: 2022 2809 2022
Size: 787 4977
ScaI AGT'ACT
Cuts at: 2257 5281 2257
Size: 3024 2740
SfcI C'TryA_G
Cuts at: 33 243 523 1075 1263 1648 1720 2018 2407
Size: 210 280 552 188 385 72 298 389
Cuts at: 2407 2805 3490 4485 4676 33
Size: 398 685 995 191 1121
Fragments arranged by size:
1121 995 685 552 398 389 385 298
280 210 191 188 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 1519 1519
Size: 5764
SfoI GGC'GCC
Cuts at: 3194 3442 3194
Size: 248 5516
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5764
SmaI CCC'GGG
Cuts at: 2784 2784
Size: 5764
SmlI C'TyrA_G
Cuts at: 1486 2416 2554 2917 3832 4326 4588 1486
Size: 930 138 363 915 494 262 2662
Fragments arranged by size:
2662 930 915 494 363 262 138
SnaBI TAC'GTA
Cuts at: 2754 2754
Size: 5764
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5764
SphI G_CATG'C
Cuts at: 2815 3315 2815
Size: 500 5264
StyI C'CwwG_G
Cuts at: 599 839 1022 2447 2896 3250 599
Size: 240 183 1425 449 354 3113
Fragments arranged by size:
3113 1425 449 354 240 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1237 1761 2291 3345 3478 3937 4148 1237
Size: 524 530 1054 133 459 211 2853
Fragments arranged by size:
2853 1054 530 524 459 211 133
TatI w'GTAC_w
Cuts at: 1595 2255 5279 1595
Size: 660 3024 2080
Fragments arranged by size:
3024 2080 660
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2629 2661 5281 5313 2629
Size: 32 2620 32 3080
Fragments arranged by size:
3080 2620 32 32
Tth111I GACn'n_nGTC
Cuts at: 3559 3559
Size: 5764
XbaI T'CTAG_A
Cuts at: 2793 2793
Size: 5764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1227 3257 1227
Size: 2030 3734
XhoI C'TCGA_G
Cuts at: 2416 2416
Size: 5764
XmaI C'CCGG_G
Cuts at: 2782 2782
Size: 5764
XmnI GAAnn'nnTTC
Cuts at: 206 2539 206
Size: 2333 3431
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI ApaI AscI AvrII BaeI
BbsI BcgI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NsiI PacI
PasI PshAI PsiI PspOMI PsrI SacII SanDI SexAI
SgrAI SrfI SspI StuI SwaI ZraI