Restriction map for
pF1KSDA0775
(Circular) (Six-base) MAPSORT of: pF1KSDA0775.seq Check: 3772 from: 1 to: 4984
pF1KSDA0775 4984 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 608 608
Size: 4984
AccI GT'mk_AC
Cuts at: 338 2020 338
Size: 1682 3302
Acc65I G'GTAC_C
Cuts at: 1913 1998 1913
Size: 85 4899
AclI AA'CG_TT
Cuts at: 4824 4824
Size: 4984
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 2807 3239 3988 4256 928
Size: 1879 432 749 268 1656
Fragments arranged by size:
1879 1656 749 432 268
AflIII A'CryG_T
Cuts at: 230 2334 3440 4417 230
Size: 2104 1106 977 797
Fragments arranged by size:
2104 1106 977 797
AgeI A'CCGG_T
Cuts at: 3372 3372
Size: 4984
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1681 1681
Size: 4984
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 121 153 121
Size: 32 4952
AlwNI CAG_nnn'CTG
Cuts at: 630 939 2287 3856 630
Size: 309 1348 1569 1758
Fragments arranged by size:
1758 1569 1348 309
ApaI G_GGCC'C
Cuts at: 585 933 585
Size: 348 4636
ApaLI G'TGCA_C
Cuts at: 3754 3754
Size: 4984
ApoI r'AATT_y
Cuts at: 1002 1986 4337 4593 1002
Size: 984 2351 256 1393
Fragments arranged by size:
2351 1393 984 256
AscI GG'CGCG_CC
Cuts at: 1644 1644
Size: 4984
AseI AT'TA_AT
Cuts at: 20 20
Size: 4984
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4984
AvaI C'yCGr_G
Cuts at: 845 926 1187 1592 1780 2002 845
Size: 81 261 405 188 222 3827
Fragments arranged by size:
3827 405 261 222 188 81
BamHI G'GATC_C
Cuts at: 2007 2007
Size: 4984
BanI G'GyrC_C
Cuts at: 1329 1374 1543 1738 1913 1998 2412 2660 2695
Size: 45 169 195 175 85 414 248 35
Cuts at: 2695 1329
Size: 3618
Fragments arranged by size:
3618 414 248 195 175 169 85 45
35
BanII G_rGCy'C
Cuts at: 585 679 775 855 933 1613 1867 1996 3026
Size: 94 96 80 78 680 254 129 1030
Cuts at: 3026 585
Size: 2543
Fragments arranged by size:
2543 1030 680 254 129 96 94 80
78
BbeI G_GCGC'C
Cuts at: 1547 2416 2664 1547
Size: 869 248 3867
Fragments arranged by size:
3867 869 248
BbvCI CC'TCA_GC
Cuts at: 568 1633 568
Size: 1065 3919
BciVI GTATCCnnnnn_n'
Cuts at: 2880 3643 4984 2880
Size: 763 1341 2880
Fragments arranged by size:
2880 1341 763
BclI T'GATC_A
Cuts at: 359 993 2502 359
Size: 634 1509 2841
Fragments arranged by size:
2841 1509 634
BglI GCCn_nnn'nGGC
Cuts at: 850 1192 1573 850
Size: 342 381 4261
Fragments arranged by size:
4261 381 342
BglII A'GATC_T
Cuts at: 608 1967 2497 4536 608
Size: 1359 530 2039 1056
Fragments arranged by size:
2039 1359 1056 530
BlpI GC'TnA_GC
Cuts at: 854 2094 854
Size: 1240 3744
Bme1580I G_kGCm'C
Cuts at: 585 933 1168 1207 1250 1258 1379 1712 1741
Size: 348 235 39 43 8 121 333 29
Cuts at: 1741 2607 2700 3758 585
Size: 866 93 1058 1811
Fragments arranged by size:
1811 1058 866 348 333 235 121 93
43 39 29 8
BmrI ACTGGGnnnn_n'
Cuts at: 776 1155 2194 2275 2610 4756 776
Size: 379 1039 81 335 2146 1004
Fragments arranged by size:
2146 1039 1004 379 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3349 3349
Size: 4984
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 442 634 817 859 1975 319
Size: 123 192 183 42 1116 3328
Fragments arranged by size:
3328 1116 192 183 123 42
Bpu10I CC'TnA_GC
Cuts at: 568 673 1633 568
Size: 105 960 3919
Fragments arranged by size:
3919 960 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 771 1101 2158 3037 3531 3829 771
Size: 330 1057 879 494 298 1926
Fragments arranged by size:
1926 1057 879 494 330 298
BsaI GGTCTCn'nnnn_
Cuts at: 203 916 4688 203
Size: 713 3772 499
Fragments arranged by size:
3772 713 499
BsaAI yAC'GTr
Cuts at: 1479 1974 2335 1479
Size: 495 361 4128
Fragments arranged by size:
4128 495 361
BsaBI GATnn'nnATC
Cuts at: 6 2496 4535 6
Size: 2490 2039 455
Fragments arranged by size:
2490 2039 455
BsaHI Gr'CG_yC
Cuts at: 324 1544 2413 2661 3365 4861 324
Size: 1220 869 248 704 1496 447
Fragments arranged by size:
1496 1220 869 704 447 248
BsaWI w'CCGG_w
Cuts at: 2171 2692 3372 3646 3793 4204 2171
Size: 521 680 274 147 411 2951
Fragments arranged by size:
2951 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 121 151 220 250 1684 1714 1930 1960 121
Size: 30 69 30 1434 30 216 30 3145
Fragments arranged by size:
3145 1434 216 69 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 880 1048 1705 391
Size: 489 168 657 3670
Fragments arranged by size:
3670 657 489 168
BseYI C'CCAG_C
Cuts at: 288 1038 1229 1351 1381 1468 1723 1896 2407
Size: 750 191 122 30 87 255 173 511
Cuts at: 2407 3165 3744 4240 288
Size: 758 579 496 1032
Fragments arranged by size:
1032 758 750 579 511 496 255 191
173 122 87 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 687 703 687
Size: 16 4968
BsiEI CG_ry'CG
Cuts at: 104 2524 2570 3780 104
Size: 2420 46 1210 1308
Fragments arranged by size:
2420 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 238 1613 1820 1996 2774 2964 3758 238
Size: 1375 207 176 778 190 794 1464
Fragments arranged by size:
1464 1375 794 778 207 190 176
BsmBI CGTCTCn'nnnn_
Cuts at: 586 1849 586
Size: 1263 3721
Bsp1286I G_dGCh'C
Cuts at: 238 585 679 775 855 933 1168 1207 1250
Size: 347 94 96 80 78 235 39 43
Cuts at: 1250 1258 1379 1613 1712 1741 1820 1867 1996
Size: 8 121 234 99 29 79 47 129
Cuts at: 1996 2607 2700 2774 2964 3026 3758 238
Size: 611 93 74 190 62 732 1464
Fragments arranged by size:
1464 732 611 347 235 234 190 129
121 99 96 94 93 80 79 78
74 62 47 43 39 29 8
BspEI T'CCGG_A
Cuts at: 4204 4204
Size: 4984
BspHI T'CATG_A
Cuts at: 4160 4160
Size: 4984
BspMI ACCTGCnnnn'nnnn_
Cuts at: 608 725 2032 2246 2548 2929 608
Size: 117 1307 214 302 381 2663
Fragments arranged by size:
2663 1307 381 302 214 117
BsrBI CCG'CTC
Cuts at: 50 801 891 1192 1238 1277 1322 3274 3328
Size: 751 90 301 46 39 45 1952 54
Cuts at: 3328 4814 4979 50
Size: 1486 165 55
Fragments arranged by size:
1952 1486 751 301 165 90 55 54
46 45 39
BsrDI GCAATG_nn'
Cuts at: 2894 2894
Size: 4984
BsrFI r'CCGG_y
Cuts at: 1043 1326 1486 2980 3161 3372 1043
Size: 283 160 1494 181 211 2655
Fragments arranged by size:
2655 1494 283 211 181 160
BssHII G'CGCG_C
Cuts at: 1449 1644 1449
Size: 195 4789
BssSI C'ACGA_G
Cuts at: 3253 3613 3253
Size: 360 4624
BstAPI GCAn_nnn'nTGC
Cuts at: 630 2359 630
Size: 1729 3255
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4984
BstYI r'GATC_y
Cuts at: 1 608 742 936 1967 2007 2474 2497 2832
Size: 607 134 194 1031 40 467 23 335
Cuts at: 2832 3078 4092 4178 4190 4536 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1031 1014 607 467 449 346 335 246
194 134 86 40 23 12
BtgI C'CryG_G
Cuts at: 125 1639 125
Size: 1514 3470
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2932 3113 3276 2932
Size: 181 163 4640
Fragments arranged by size:
4640 181 163
BtsI GCAGTG_nn'
Cuts at: 260 2225 260
Size: 1965 3019
DraI TTT'AAA
Cuts at: 1981 1981
Size: 4984
DraIII CAC_nnn'GTG
Cuts at: 784 1479 784
Size: 695 4289
DrdI GACnn_nn'nnGTC
Cuts at: 2688 3548 2688
Size: 860 4124
EaeI y'GGCC_r
Cuts at: 766 1116 1405 1442 1574 1831 2567 2741 3132
Size: 350 289 37 132 257 736 174 391
Cuts at: 3132 3159 4506 766
Size: 27 1347 1244
Fragments arranged by size:
1347 1244 736 391 350 289 257 174
132 37 27
EagI C'GGCC_G
Cuts at: 2567 2567
Size: 4984
EarI CTCTTCn'nnn_
Cuts at: 541 809 3005 3215 541
Size: 268 2196 210 2310
Fragments arranged by size:
2310 2196 268 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 228 1537 3190 3502 3648 4794 228
Size: 1309 1653 312 146 1146 418
Fragments arranged by size:
1653 1309 1146 418 312 146
EcoICRI GAG'CTC
Cuts at: 1611 1994 1611
Size: 383 4601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 442 634 817 859 928 1975 2807 3239
Size: 123 192 183 42 69 1047 832 432
Cuts at: 3239 3988 4256 319
Size: 749 268 1047
Fragments arranged by size:
1047 1047 832 749 432 268 192 183
123 69 42
EcoNI CCTnn'n_nnAGG
Cuts at: 163 421 163
Size: 258 4726
EcoO109I rG'GnC_Cy
Cuts at: 171 178 294 581 582 1628 2121 171
Size: 7 116 287 1 1046 493 3034
Fragments arranged by size:
3034 1046 493 287 116 7 1
EcoRI G'AATT_C
Cuts at: 1986 1986
Size: 4984
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 520 570 1506 3001 520
Size: 50 936 1495 2503
Fragments arranged by size:
2503 1495 936 50
FspI TGC'GCA
Cuts at: 2763 2763
Size: 4984
HaeII r_GCGC'y
Cuts at: 1012 1236 1275 1320 1365 1547 2416 2664 3688
Size: 224 39 45 45 182 869 248 1024
Cuts at: 3688 4667 1012
Size: 979 1329
Fragments arranged by size:
1329 1024 979 869 248 224 182 45
45 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 220 252 314 346 500 532 928 960 2824
Size: 32 62 32 154 32 396 32 1864
Cuts at: 2824 2854 2856 2886 220
Size: 30 2 30 2318
Fragments arranged by size:
2318 1864 396 154 62 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2021 2021
Size: 4984
KasI G'GCGC_C
Cuts at: 1543 2412 2660 1543
Size: 869 248 3867
Fragments arranged by size:
3867 869 248
KpnI G_GTAC'C
Cuts at: 1917 2002 1917
Size: 85 4899
MluI A'CGCG_T
Cuts at: 4417 4417
Size: 4984
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 345 692 935 971 1667 1892 3655 3839 345
Size: 347 243 36 696 225 1763 184 1490
Fragments arranged by size:
1763 1490 696 347 243 225 184 36
MscI TGG'CCA
Cuts at: 2743 2743
Size: 4984
MslI CAynn'nnrTG
Cuts at: 2971 3098 2971
Size: 127 4857
MspA1I CmG'CkG
Cuts at: 630 915 1134 1385 1641 1748 1880 1896 2093
Size: 285 219 251 256 107 132 16 197
Cuts at: 2093 2407 2767 3782 4027 630
Size: 314 360 1015 245 1587
Fragments arranged by size:
1587 1015 360 314 285 256 251 245
219 197 132 107 16
NaeI GCC'GGC
Cuts at: 1045 1488 3163 1045
Size: 443 1675 2866
Fragments arranged by size:
2866 1675 443
NarI GG'CG_CC
Cuts at: 1544 2413 2661 1544
Size: 869 248 3867
Fragments arranged by size:
3867 869 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4984
NgoMIV G'CCGG_C
Cuts at: 1043 1486 3161 1043
Size: 443 1675 2866
Fragments arranged by size:
2866 1675 443
NruI TCG'CGA
Cuts at: 3345 3345
Size: 4984
NspI r_CATG'y
Cuts at: 234 2035 2535 3444 234
Size: 1801 500 909 1774
Fragments arranged by size:
1801 1774 909 500
PasI CC'CwG_GG
Cuts at: 174 174
Size: 4984
PciI A'CATG_T
Cuts at: 230 3440 230
Size: 3210 1774
PflMI CCAn_nnn'nTGG
Cuts at: 939 939
Size: 4984
PfoI T'CCnGG_A
Cuts at: 204 650 860 204
Size: 446 210 4328
Fragments arranged by size:
4328 446 210
PmeI GTTT'AAAC
Cuts at: 1981 1981
Size: 4984
PmlI CAC'GTG
Cuts at: 1479 1479
Size: 4984
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 121 153 4123 4155 121
Size: 32 3970 32 950
Fragments arranged by size:
3970 950 32 32
PspOMI G'GGCC_C
Cuts at: 581 929 581
Size: 348 4636
PstI C_TGCA'G
Cuts at: 446 899 920 1565 2029 2714 446
Size: 453 21 645 464 685 2716
Fragments arranged by size:
2716 685 645 464 453 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4984
PvuII CAG'CTG
Cuts at: 630 915 1385 1880 1896 2407 2767 630
Size: 285 470 495 16 511 360 2847
Fragments arranged by size:
2847 511 495 470 360 285 16
RsrII CG'GwC_CG
Cuts at: 3177 3177
Size: 4984
SacI G_AGCT'C
Cuts at: 1613 1996 1613
Size: 383 4601
SacII CC_GC'GG
Cuts at: 1642 1642
Size: 4984
SalI G'TCGA_C
Cuts at: 2019 2019
Size: 4984
SapI GCTCTTCn'nnn_
Cuts at: 3005 3215 3005
Size: 210 4774
SbfI CC_TGCA'GG
Cuts at: 2029 2029
Size: 4984
ScaI AGT'ACT
Cuts at: 4501 4501
Size: 4984
SfcI C'TryA_G
Cuts at: 33 442 895 916 1066 1561 2025 2710 3705
Size: 409 453 21 150 495 464 685 995
Cuts at: 3705 3896 33
Size: 191 1121
Fragments arranged by size:
1121 995 685 495 464 453 409 191
150 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 1573 1573
Size: 4984
SfoI GGC'GCC
Cuts at: 1545 2414 2662 1545
Size: 869 248 3867
Fragments arranged by size:
3867 869 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4984
SgrAI Cr'CCGG_yG
Cuts at: 1326 1326
Size: 4984
SmaI CCC'GGG
Cuts at: 847 928 1594 2004 847
Size: 81 666 410 3827
Fragments arranged by size:
3827 666 410 81
SmlI C'TyrA_G
Cuts at: 750 1080 2137 3052 3546 3808 750
Size: 330 1057 915 494 262 1926
Fragments arranged by size:
1926 1057 915 494 330 262
SnaBI TAC'GTA
Cuts at: 1974 1974
Size: 4984
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4984
SphI G_CATG'C
Cuts at: 2035 2535 2035
Size: 500 4484
StuI AGG'CCT
Cuts at: 523 647 523
Size: 124 4860
StyI C'CwwG_G
Cuts at: 125 2116 2470 125
Size: 1991 354 2639
Fragments arranged by size:
2639 1991 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 795 963 2565 2698 3157 3368 795
Size: 168 1602 133 459 211 2411
Fragments arranged by size:
2411 1602 459 211 168 133
TatI w'GTAC_w
Cuts at: 4499 4499
Size: 4984
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1684 1716 1810 1842 1930 1962 4501 4533 1684
Size: 32 94 32 88 32 2539 32 2135
Fragments arranged by size:
2539 2135 94 88 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2779 2779
Size: 4984
XbaI T'CTAG_A
Cuts at: 2013 2013
Size: 4984
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1731 2477 1731
Size: 746 4238
XmaI C'CCGG_G
Cuts at: 845 926 1592 2002 845
Size: 81 666 410 3827
Fragments arranged by size:
3827 666 410 81
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AvrII BaeI BbsI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NsiI PacI
PpuMI PshAI PsiI PspXI PsrI SanDI SexAI SrfI
SspI SwaI XhoI XmnI ZraI